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Pesquisa : D06.472.334.851.437 [Categoria DeCS]
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[PMID]:28743541
[Au] Autor:Perkins MS; Louw-du Toit R; Africander D
[Ad] Endereço:Department of Biochemistry, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa. Electronic address: meghan@sun.ac.za.
[Ti] Título:A comparative characterization of estrogens used in hormone therapy via estrogen receptor (ER)-α and -ß.
[So] Source:J Steroid Biochem Mol Biol;174:27-39, 2017 11.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Conventional hormone therapy (HT) containing estrogens such as ethinylestradiol (EE) have been associated with an increased risk of breast cancer and cardiovascular disease resulting in women seeking safer alternatives that are claimed to have fewer health risks. One such alternative gaining popularity, is custom-compounded bioidentical (b)HT formulations containing bioidentical estradiol (bE ) and estriol (bE ). However, the preparation of these custom-compounded estrogens is not regulated, and depending on the route of synthesis, steroid mixtures with differing activities may be produced. Thus, an investigation into the activities of estrogens prepared by custom-compounded pharmacies is warranted. The aim of this study was therefore to directly compare the pharmacological properties of bE and bE of unknown purity relative to commercially available, pure E , E and estrone (E ) standards as well as synthetic EE used in conventional HT via the human estrogen receptor (ER)-α and -ß. We determined precise equilibrium dissociation constants (K or K values) and showed that bE and bE display similar binding affinities to the E and E standards, while EE had a higher affinity for ERα, and E a lower affinity for ERß. Furthermore, all the estrogens display similar agonist efficacies, but not potencies, for transactivation on a minimal ERE-containing promoter via the individual ER subtypes. Although E and E were equally efficacious and potent on the endogenous ERE-containing pS2 promoter in the MCF-7 BUS breast cancer cell line co-expressing ERα and ERß, E was less efficacious and potent than E . This study is the first to demonstrate that the bioidentical estrogens, commercially available estrogen standards and synthetic EE are full agonists for transrepression on both minimal and endogenous NFκB-containing promoters. Moreover, we showed that these estrogens all increase proliferation and anchorage-independent growth of MCF-7 BUS cells to a similar extent, suggesting that custom-compounded bHT may in fact not be a safer alternative to conventional HT. Furthermore, our results showing that E and E are not weak estrogens, and that E does not antagonize the activity of E , suggest that the rationale behind the use of E and E in custom-compounded bHT formulations should be readdressed. Taken together, the results indicating that there is mostly no difference between the custom-compounded bioidentical estrogens, commercially available estrogen standards and synthetic EE, at concentrations reflecting serum levels in women using estrogen-containing HT, suggest that there is no clear advantage in choosing bHT above conventional HT.
[Mh] Termos MeSH primário: Congêneres do Estradiol/farmacologia
Receptor alfa de Estrogênio/metabolismo
Receptor beta de Estrogênio/metabolismo
Estrogênios/farmacologia
[Mh] Termos MeSH secundário: Animais
Ligação Competitiva
Células COS
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Cercopithecus aethiops
Receptor alfa de Estrogênio/genética
Receptor beta de Estrogênio/genética
Expressão Gênica/efeitos dos fármacos
Células HEK293
Terapia de Reposição Hormonal
Seres Humanos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Estradiol Congeners); 0 (Estrogen Receptor alpha); 0 (Estrogen Receptor beta); 0 (Estrogens); 0 (estrogen receptor alpha, human)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171208
[Lr] Data última revisão:
171208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


  2 / 2174 MEDLINE  
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[PMID]:28441746
[Au] Autor:Asako Y; Uesawa Y
[Ad] Endereço:Department of Clinical Pharmaceutics Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan. y121007@std.my-pharm.ac.jp.
[Ti] Título:High-Performance Prediction of Human Estrogen Receptor Agonists Based on Chemical Structures.
[So] Source:Molecules;22(4), 2017 Apr 23.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Many agonists for the estrogen receptor are known to disrupt endocrine functioning. We have developed a computational model that predicts agonists for the estrogen receptor ligand-binding domain in an assay system. Our model was entered into the Tox21 Data Challenge 2014, a computational toxicology competition organized by the National Center for Advancing Translational Sciences. This competition aims to find high-performance predictive models for various adverse-outcome pathways, including the estrogen receptor. Our predictive model, which is based on the random forest method, delivered the best performance in its competition category. In the current study, the predictive performance of the random forest models was improved by strictly adjusting the hyperparameters to avoid overfitting. The random forest models were optimized from 4000 descriptors simultaneously applied to 10,000 activity assay results for the estrogen receptor ligand-binding domain, which have been measured and compiled by Tox21. Owing to the correlation between our model's and the challenge's results, we consider that our model currently possesses the highest predictive power on agonist activity of the estrogen receptor ligand-binding domain. Furthermore, analysis of the optimized model revealed some important features of the agonists, such as the number of hydroxyl groups in the molecules.
[Mh] Termos MeSH primário: Congêneres do Estradiol/química
Receptores Estrogênicos/química
[Mh] Termos MeSH secundário: Área Sob a Curva
Árvores de Decisões
Seres Humanos
Aprendizado de Máquina
Modelos Químicos
Relação Quantitativa Estrutura-Atividade
Curva ROC
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estradiol Congeners); 0 (Receptors, Estrogen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE


  3 / 2174 MEDLINE  
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[PMID]:28384514
[Au] Autor:Wang J; Zhu Y
[Ad] Endereço:College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China. Electronic address: wangjun2016@scau.edu.cn.
[Ti] Título:Occurrence and risk assessment of estrogenic compounds in the East Lake, China.
[So] Source:Environ Toxicol Pharmacol;52:69-76, 2017 Jun.
[Is] ISSN:1872-7077
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Forty two surface water samples were collected in May and June of 2013 in five lakelets of the East Lake, China. Four steroid hormones (17ß-estradiol (ßE2), estrone (E1), 17α-estradiol (αE2) and 17α-ethinylestradiol (αEE2)) were analyzed in these samples. Determination of four estrogenic compounds was performed on high performance liquid chromatography/mass spectrometry (HPLC/MS). ßE2 was detected with the highest detection frequency of 62% and its concentration range was from nd to 17.58ng/L. The mean total concentration of four compounds increased with the order: Houhu lake (L5) (8.91ng/L)
[Mh] Termos MeSH primário: Congêneres do Estradiol/análise
Estrogênios/análise
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Animais
China
Monitoramento Ambiental
Fazendas
Peixes
Lagos
Medição de Risco
Águas Residuais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estradiol Congeners); 0 (Estrogens); 0 (Waste Water); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE


  4 / 2174 MEDLINE  
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[PMID]:28363417
[Au] Autor:Socas-Rodríguez B; Herrera-Herrera AV; Hernández-Borges J; Rodríguez-Delgado MÁ
[Ad] Endereço:Departamento de Química, Unidad Departamental de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez, s/n°, 38206 San Cristóbal de La Laguna, Spain.
[Ti] Título:Multiresidue determination of estrogens in different dairy products by ultra-high-performance liquid chromatography triple quadrupole mass spectrometry.
[So] Source:J Chromatogr A;1496:58-67, 2017 May 05.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this work, a simple and fast methodology has been validated and applied for the analysis of a group of 22 estrogenic compounds including eight phytoestrogens (i.e. daidzein, enterodiol, glycitein, enterolactone, genistein, formononetin, prunetin, biochanin A), six mycotoxins (ß-zearalanol, ß-zearalenol, α-zearalanol, α-zearalenol, zearalanone, zearalenone) as well as four synthetic (i.e. ethynylestradiol, diethylstilbestrol, dienestrol, hexestrol) and four natural estrogens (i.e. estriol, 17ß-estradiol, 17α-estradiol, estrone) in different dairy products. Extraction was carried out using the QuEChERS method while separation, determination and quantification of the target analytes were achieved by ultra-high-performance liquid chromatography coupled to triple quadrupole mass spectrometry with an electrospray ionization interface. The methodology was validated for four dairy product samples with relevant interest for the population including skimmed and whole cheese and goat and cow kefir, using 17ß-estradiol-2,4,16,16,17-d as internal standard for natural and synthetic estrogens and ß-zeralanol-10,10,11,12,12-d as internal standard for mycotoxins and phytoestrogens. Recovery ranged from 70 to 119% for the four types of matrices with RSD values lower than 14% and the limits of quantification of the method achieved were in the range 0.025-2.50µg/kg for all samples. Finally, the analysis of commercially available products was carried out finding the presence of daidzein, glycitein enterolactone and genistein in some of the studied samples.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão
Laticínios/análise
Estrogênios/análise
Espectrometria de Massas/métodos
[Mh] Termos MeSH secundário: Animais
Bovinos
Congêneres do Estradiol/análise
Micotoxinas/análise
Fitoestrógenos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Estradiol Congeners); 0 (Estrogens); 0 (Mycotoxins); 0 (Phytoestrogens)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170402
[St] Status:MEDLINE


  5 / 2174 MEDLINE  
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[PMID]:27677392
[Au] Autor:Wang Z; Li R; Wu F; Feng C; Ye C; Yan C
[Ad] Endereço:Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Boulevard, Xiamen 361021, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Elec
[Ti] Título:Estrogenic compound profiles in an urbanized industry-impacted coastal bay and potential risk assessment by pollution indices and multivariative statistical methods.
[So] Source:Mar Pollut Bull;114(1):397-407, 2017 Jan 15.
[Is] ISSN:1879-3363
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The occurrence and distribution of target estrogenic compounds in a highly urbanized industry-impacted coastal bay were investigated, and contamination profiles were evaluated by estimating total estradiol equivalents (∑EEQs) and risk quotients (RQs). Phenolic compounds were the most abundant xenoestrogens, but seldom showed contribution to the ∑EEQs. The diethylstilbestrol (DES) and 17α-ethinylestradiol (EE2) were the major contributors followed by 17ß-estradiol (E2) in comparison with a slight contribution from estrone (E1) and estriol (E3). Both ∑EEQs and RQs indicated likely adverse effects posed on resident organisms. Further, multivariate statistical method comprehensively revealed pollution status by visualized factor scores and identified multiple "hotspots" of estrogenic sources, demonstrating the presence of complex pollution risk gradients inside and particularly outside of bay area. Overall, this study favors the integrative utilization of pollution indices and factor analysis as powerful tool to scientifically diagnose the pollution characterization of human-derived chemicals for better management decisions in aquatic environments.
[Mh] Termos MeSH primário: Baías/química
Monitoramento Ambiental/métodos
Estrogênios/análise
Urbanização
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: China
Monitoramento Ambiental/estatística & dados numéricos
Congêneres do Estradiol/análise
Estrogênios não Esteroides/análise
Análise Multivariada
Fenóis/análise
Medição de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estradiol Congeners); 0 (Estrogens); 0 (Estrogens, Non-Steroidal); 0 (Phenols); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160929
[St] Status:MEDLINE


  6 / 2174 MEDLINE  
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[PMID]:27510162
[Au] Autor:Silva LLS; Sales JCS; Campos JC; Bila DM; Fonseca FV
[Ad] Endereço:Escola de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos, 149, Rio de Janeiro, Brazil. larissaloureiross@hotmail.com.
[Ti] Título:Advanced oxidative processes and membrane separation for micropollutant removal from biotreated domestic wastewater.
[So] Source:Environ Sci Pollut Res Int;24(7):6329-6338, 2017 Mar.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The presence of micropollutants in sewage is already widely known, as well as the effects caused by natural and synthetic hormones. Thus, it is necessary to apply treatments to remove them from water systems, such as advanced oxidation processes (AOPs) and membrane separation processes, which can oxidize and remove high concentrations of organic compounds. This work investigated the removal of 17ß-estradiol (E2), 17α-ethinylestradiol (EE2), and estriol (E3) from biotreated sewage. Reverse osmosis processes were conducted at three recoveries (50, 60, and 70 %). For E2 and EE2, the removals were affected by the recovery. The best results for RO were as follows: the E2 compound removal was 89 % for 60 % recovery and the EE2 compound removal was 57 % for 50 % recovery. The RO recovery did not impact the E3 removal. It was concluded that the interaction between the evaluated estrogens, and the membrane was the major factor for the hormone separation. The AOP treatment using H O /UV was carried out in two sampling campaigns. First, we evaluated the variation of UV doses (24.48, 73.44, 122.4, and 244.8 kJ m ) with 18.8 mg L of H O in the reaction. EE2 showed considerable removals (around 70 %). In order to optimize the results, an experimental design was applied. The best result was obtained with higher UV dose (122.4 kJ m ) and lower H O concentration (4 mg L ), achieving removal of 91 % for E3 and 100 % for E2 and EE2.
[Mh] Termos MeSH primário: Congêneres do Estradiol/química
Congêneres do Estradiol/isolamento & purificação
Membranas Artificiais
Esgotos/química
Poluentes Químicos da Água/química
Poluentes Químicos da Água/isolamento & purificação
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Peróxido de Hidrogênio/química
Oxirredução
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estradiol Congeners); 0 (Membranes, Artificial); 0 (Sewage); 0 (Water Pollutants, Chemical); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160812
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-7312-y


  7 / 2174 MEDLINE  
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[PMID]:27863841
[Au] Autor:Bronowicka-Klys DE; Lianeri M; Jagodzinski PP
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poland. Electronic address: d.e.bronowicka@gmail.com.
[Ti] Título:The role and impact of estrogens and xenoestrogen on the development of cervical cancer.
[So] Source:Biomed Pharmacother;84:1945-1953, 2016 Dec.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Throughout an individual's lifetime, the human body is exposed to many different chemical compounds, including xenoestrogens (XEs) that can be found in the environment, food, air, cosmetics and other substances, which have a positive or negative impact on their health and lifestyle. Whereas high-risk human papillomavirus (HR-HPV) is necessary but not sufficient for full malignant cervical cell transformation, other compounds such as estrogens and XEs may be risk factors for cervical cancer (CC) development. The causes and effects of some diseases such as cancer, cardiovascular, metabolic or immune system disorders are partly due to signaling pathways in response to estrogens. XEs are a vast group of natural and synthetic compounds, behaving like estrogens, that have been studied over the recent years and which may interact with estrogen receptors. The major problem with XEs is the difficulty in studying the mechanism of such complex substances as well as investigating the influences of some of the compounds (dose-dependent) over time. The impact of XEs on CC is variable, with no direct comparison between in vitro studies and in vivo XEs action.
[Mh] Termos MeSH primário: Exposição Ambiental/efeitos adversos
Congêneres do Estradiol/efeitos adversos
Congêneres do Estradiol/metabolismo
Estrogênios/efeitos adversos
Estrogênios/metabolismo
Neoplasias do Colo do Útero/metabolismo
[Mh] Termos MeSH secundário: Animais
Congêneres do Estradiol/química
Estrogênios/química
Feminino
Seres Humanos
Receptores Estrogênicos/metabolismo
Transdução de Sinais/fisiologia
Neoplasias do Colo do Útero/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Estradiol Congeners); 0 (Estrogens); 0 (Receptors, Estrogen)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE


  8 / 2174 MEDLINE  
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[PMID]:27688174
[Au] Autor:He XP; Lian ZR; Tan LJ; Wang JT
[Ad] Endereço:Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, PR China.
[Ti] Título:Preparation and characterization of magnetic molecularly imprinted polymers for selective trace extraction of dienestrol in seawater.
[So] Source:J Chromatogr A;1469:8-16, 2016 Oct 21.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Highly selective and efficient magnetic molecularly imprinted polymers (MMIPs) were prepared using Fe O @SiO as a magnetic supporter, 3-methacryloxypropyltrimethoxy-silane (MPS) as a silane coupling agent, DIS as a template, methacrylic acid (MAA) as a functional monomer and ethyleneglycol dimethacrylate (EGDMA) as a cross-linker for the extraction of trace residuals of the synthetic estrogen dienestrol (DIS) in seawater, which is a concern worldwide for its endocrine disruption and carcinogenic danger to human health. The obtained MMIPs were demonstrated to have spherical morphologies, core-shell structures, large binding capacities, high efficiency and selectivity. These were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and adsorption experiments. Owing to the specific binding sites, the MMIPs exhibited an almost three times higher adsorption capacity towards DIS (Q =4.68mgg ) than magnetic molecularly non-imprinted polymers (MNIPs) (Q =1.72mgg ). DIS in spiked seawater samples from the Weihai Bay of China was extracted and enriched by MMIPs, and satisfactory recoveries (87.3%-96.4%) with low relative standard deviation (RSD) values (2.03%-5.18%, n=5) were obtained. The limit of detection (LOD) of the method obtained was 0.16µgL , and the limit of quantitation was 0.52µgL after MMIPs. No significant deterioration of the adsorption capacity of the MMIPs was observed after six rounds of regeneration. The results further demonstrated the applicability of the MMIPs method, a simple and straightforward method for the extraction and enrichment of DIS in seawater without any time-consuming procedures.
[Mh] Termos MeSH primário: Dienestrol/isolamento & purificação
Congêneres do Estradiol/isolamento & purificação
Nanopartículas de Magnetita/química
Poluentes Químicos da Água/isolamento & purificação
[Mh] Termos MeSH secundário: Adsorção
China
Seres Humanos
Limite de Detecção
Microscopia Eletrônica de Transmissão
Impressão Molecular
Água do Mar/química
Silanos/química
Dióxido de Silício/química
Microextração em Fase Sólida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estradiol Congeners); 0 (Magnetite Nanoparticles); 0 (Silanes); 0 (Water Pollutants, Chemical); 7631-86-9 (Silicon Dioxide); RRW32X4U1F (Dienestrol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161001
[St] Status:MEDLINE


  9 / 2174 MEDLINE  
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[PMID]:27562415
[Au] Autor:Goh SX; Duarah A; Zhang L; Snyder SA; Lee HK
[Ad] Endereço:Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; National University of Singapore, Environmental Research Institute, T-Lab Building #02-01, 5A Engineering Drive 1, Singapore 117411, Singapore.
[Ti] Título:Online solid phase extraction with liquid chromatography-tandem mass spectrometry for determination of estrogens and glucocorticoids in water.
[So] Source:J Chromatogr A;1465:9-19, 2016 Sep 23.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present work describes the development of a novel fully automated online solid phase extraction (SPE) coupled with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) using negative electrospray ionisation (ESI) for the simultaneous determination of six estrogens and six glucocorticoids in water. Filtered water samples (5mL) were preconcentrated on a HyperSep™ Retain PEP SPE cartridge, eluted in back-flush mode, and separated on an LC column before analysis by tandem mass spectrometry. The total analysis time for each sample was 17min. Different experimental parameters such as the type of online SPE cartridge, loading flow rate, and composition of methanol in the loading phase were optimised. The intra-day repeatability of method ranged from 1.48 to 9.68% for all analytes, and the inter-day reproducibility ranged from 2.03 to 8.63% for all analytes, except for dexamethasone at 11.95%. These were calculated based on the peak area responses of the targeted analytes spiked at 50ng/L in ultrapure water. The method also showed good linearity from 1 to 100ng/L, with the limits of detection (LODs) ranging from 0.16 to 2.14ng/L. The proposed method was applied to the analysis of municipal wastewater. This fully automated online SPE extraction coupled with LC-MS/MS method is effective and reliable to measure estrogens and glucocorticoids simultaneously due to its high throughput, relatively low solvent consumption, reusability of the online SPE cartridge, and reduction of manual labor.
[Mh] Termos MeSH primário: Cromatografia Líquida
Monitoramento Ambiental/métodos
Estrogênios/análise
Glucocorticoides/análise
Extração em Fase Sólida
Espectrometria de Massas em Tandem
Água/química
[Mh] Termos MeSH secundário: Automação Laboratorial
Congêneres do Estradiol
Limite de Detecção
Reprodutibilidade dos Testes
Águas Residuais/análise
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estradiol Congeners); 0 (Estrogens); 0 (Glucocorticoids); 0 (Waste Water); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160827
[St] Status:MEDLINE


  10 / 2174 MEDLINE  
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[PMID]:27465804
[Au] Autor:Griffith DR; Kido Soule MC; Eglinton TI; Kujawinski EB; Gschwend PM
[Ad] Endereço:Department of Chemistry, Willamette University, USA. dgriffit@willamette.edu.
[Ti] Título:Steroidal estrogen sources in a sewage-impacted coastal ocean.
[So] Source:Environ Sci Process Impacts;18(8):981-91, 2016 Aug 10.
[Is] ISSN:2050-7895
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Estrogens are known to be potent endocrine disrupting chemicals that are commonly found in wastewater effluents at ng L(-1) levels. Yet, we know very little about the distribution and fate of estrogens in coastal oceans that receive wastewater inputs. This study measured a wide range of steroidal estrogens in sewage-impacted seawater using ultra high performance liquid chromatography (UHPLC) tandem mass spectrometry (MS/MS) together with the method of standard addition. In Massachusetts Bay, we find conjugated, free, and halogenated estrogens at concentrations that are consistent with dilution at sites close to the sewage source. At a site 6 miles down current of the sewage source, we observe estrone (E1) concentrations (520 ± 180 pg L(-1)) that are nearly double the nearfield concentrations (320 ± 60 pg L(-1)) despite 9-fold dilution of carbamazepine, which was used as a conservative sewage tracer. Our results suggest that background E1 concentrations in Massachusetts Bay (∼270 ± 50 pg L(-1)) are derived largely from sources unrelated to wastewater effluent such as marine vertebrates.
[Mh] Termos MeSH primário: Disruptores Endócrinos/análise
Estrogênios/análise
Estrona/análise
Água do Mar/química
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Animais
Congêneres do Estradiol
Halogenação
Massachusetts
Oceanos e Mares
Esgotos/química
Espectrometria de Massas em Tandem
Águas Residuais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Endocrine Disruptors); 0 (Estradiol Congeners); 0 (Estrogens); 0 (Sewage); 0 (Waste Water); 0 (Water Pollutants, Chemical); 2DI9HA706A (Estrone)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160729
[St] Status:MEDLINE
[do] DOI:10.1039/c6em00127k



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