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[PMID]:28972919
[Au] Autor:Liang YQ; Huang GY; Lin Z; Li J; Yang JW; Zhong LY; Ying GG
[Ad] Endereço:Faculty of Chemistry and Environmental Science, Guangdong Ocean University, Zhanjiang, 524088, PR China. Electronic address: liangyanqiu11@126.com.
[Ti] Título:Reproductive effects of synthetic progestin norgestrel in zebrafish (Danio rerio).
[So] Source:Chemosphere;190:17-24, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to assess the adverse effects of synthetic progestin norgestrel (NGT) on the reproduction of zebrafish by measuring the egg production, histology and transcriptional expression profiles along the hypothalamic-pituitary-gonadal (HPG) axis in adult zebrafish. After a pre-exposure period of 7 days, adult zebrafish were exposed to 6, 29 and 69 ng L NGT for 21 days. The results showed that exposure to 69 ng L NGT led to a significant up-regulation of follicle stimulating hormone, beta polypeptide (fshb), luteinizing hormone, beta polypeptide (lhb), progesterone receptor (pgr), estrogen receptor 1 (esr1) and androgen receptor (ar) genes in the brains, as well as significant up-regulation of hydroxysteroid 20-beta dehydrogenase (hsd20b) and hydroxysteroid 11-beta dehydrogenase 2 (hsd11b2) genes and down-regulation of 11-beta-hydroxylase (cyp11b) gene in the ovaries of females. In the testes of males, an overall down-regulation of steroidogenic acute regulatory protein (star), cytochrome P450-mediated side-chain cleavage enzyme (cyp11a1), cyp11b, hsd20b, hydroxysteroid 17-beta dehydrogenase type 3 (hsd17b3), hsd11b2 and ar genes were observed following exposure to different treatments of NGT. These transcriptional alterations imply that NGT could exhibit the potent progestogenic and androgenic activities in zebrafish. Egg production as well as histology in the ovaries and testes was not affected by NGT. Taken together, the overall results demonstrated that NGT could significantly affect transcriptional expression levels of genes related to HPG axis in zebrafish, and whether that change translates to additional physiological effects is needed further research.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica/efeitos dos fármacos
Norgestrel/farmacologia
Reprodução/efeitos dos fármacos
Peixe-Zebra/fisiologia
[Mh] Termos MeSH secundário: Animais
Anticoncepcionais Orais Sintéticos/farmacologia
Feminino
Gonadotropinas Hipofisárias/genética
Hormônios Hipotalâmicos/genética
Masculino
Norgestrel/metabolismo
Progestinas/fisiologia
Receptores de Progesterona/genética
Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Contraceptives, Oral, Synthetic); 0 (Gonadotropins, Pituitary); 0 (Hypothalamic Hormones); 0 (Progestins); 0 (Receptors, Progesterone); 3J8Q1747Z2 (Norgestrel)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE


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[PMID]:29223873
[Au] Autor:Wang B; Liu Q; Liu X; Xu Y; Shi B
[Ad] Endereço:Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries and Food Production Processes, Qingdao National Laboratory for Marine Science a
[Ti] Título:Molecular characterization and expression profiles of LPXRFa at the brain-pituitary-gonad axis of half-smooth tongue sole (Cynoglossus semilaevis) during ovarian maturation.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;216:59-68, 2018 Feb.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Gonadotropin-inhibitory hormone (GnIH) has been characterized by its ability to inhibit either basal or gonadotropin-releasing hormone (GnRH)-induced gonadotropin synthesis and release in birds and mammals. However, the physiological role of GnIH on the reproductive axis in fish remains inconclusive, with most studies focusing on the orders Cypriniformes and Perciformes. To gain insight into the role of GnIH in the regulation of reproduction in the order Pleuronectiformes, we first cloned the LPXRFa gene, the piscine ortholog of GnIH, in the half-smooth tongue sole. The full-length cDNA of LPXRFa was 918bp in size with an open reading frame (ORF) of 585bp that encoded a 194 amino acids preprohormone with a calculated molecular mass and isoelectric point of 21.73kDa and 6.52, respectively. The LPXRFa precursor encoded two putative peptide sequences that included -MPMRF or -MPQRF motifs at the C-terminal. Tissue distribution analysis showed that LPXRFa transcripts could be detected at high levels in the brains of both sexes and to a lesser extent in the ovary, heart and stomach of females, while a noteworthy expression was observed in the kidney and muscle of males. Furthermore, the expression patterns of LPXRFa mRNA during ovarian maturation were also investigated. In the brain, the mRNA expression of LPXRFa increased significantly at stage III, declined at stage V and reached a maximum at stage VI. In the pituitary, the levels of LPXRFa mRNA remained stable during ovarian maturation and increased significantly to the top level at stage V and then declined back to basal levels. In contrast, the ovarian LPXRFa mRNA levels declined sharply at stage III and remained depressed over the course of ovarian maturation. Taken together, our results provide further evidence for the existence of LPXRFa in the order Pleuronectiformes and suggest its possible involvement in the regulation of reproduction in the female tongue sole.
[Mh] Termos MeSH primário: Proteínas de Peixes
Peixes
Regulação da Expressão Gênica/fisiologia
Hormônios Hipotalâmicos
Ovário/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Clonagem Molecular
Feminino
Proteínas de Peixes/biossíntese
Proteínas de Peixes/genética
Peixes/genética
Peixes/crescimento & desenvolvimento
Hormônios Hipotalâmicos/biossíntese
Hormônios Hipotalâmicos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Hypothalamic Hormones)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE


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[PMID]:28911868
[Au] Autor:Prinz P; Scharner S; Friedrich T; Schalla M; Goebel-Stengel M; Rose M; Stengel A
[Ad] Endereço:Department for Psychosomatic Medicine, Charité-Universitätsmedizin Berlin, Germany.
[Ti] Título:Central and peripheral expression sites of phoenixin-14 immunoreactivity in rats.
[So] Source:Biochem Biophys Res Commun;493(1):195-201, 2017 Nov 04.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phoenixin is a pleiotropic peptide involved in reproduction, anxiety and recently also implicated in the control of food intake. Besides the 20-amino acid phoenixin, the 14-amino acid phoenixin-14 also shows bioactive properties. However, the expression sites of phoenixin-14 in the brain and peripheral tissues are not yet described in detail. Therefore, a mapping of the brain and peripheral tissues from male and female Sprague-Dawley rats with a specific phoenixin-14 antibody was performed using western blot and immunohistochemistry. High density of phoenixin-14 immunoreactivity was detected in the medial division of the brain central amygdaloid nucleus, in the spinal trigeminal tract and in the spinocerebellar tract as well as in cells between the crypts of duodenum, jejunum and ileum. Medium density immunoreactivity was observed in the bed nucleus of the stria terminalis, in the area postrema, the nucleus of the solitary tract and the dorsal motor nucleus of the vagus nerve as well as in the peripheral parts of the islets of Langerhans in the pancreas. A low density of phoenixin-14 immunoreactivity was detected in the arcuate nucleus, the supraoptic nucleus and the raphe pallidus. After pre-absorption of the antibody with phoenixin-14 peptide, no immunosignals were observed indicating specificity of the antibody. Taken together, the widespread distribution of phoenixin-14 immunoreactivity gives additional rise to the pleiotropic functions of the peptide such as possible effects in gastrointestinal motility, immune functions and glucose homeostasis.
[Mh] Termos MeSH primário: Encéfalo/imunologia
Hormônios Hipotalâmicos/imunologia
Intestinos/imunologia
Hormônios Peptídicos/imunologia
Medula Espinal/imunologia
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Especificidade de Órgãos/imunologia
Ratos
Ratos Sprague-Dawley
Caracteres Sexuais
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypothalamic Hormones); 0 (Peptide Hormones); 0 (phoenixin-14, rat)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE


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[PMID]:28570646
[Au] Autor:McGregor R; Shan L; Wu MF; Siegel JM
[Ad] Endereço:Department of Psychiatry and Biobehavioral Sciences, University of California at Los Angeles, Los Angeles, California, United States of America.
[Ti] Título:Diurnal fluctuation in the number of hypocretin/orexin and histamine producing: Implication for understanding and treating neuronal loss.
[So] Source:PLoS One;12(6):e0178573, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The loss of specific neuronal phenotypes, as determined by immunohistochemistry, has become a powerful tool for identifying the nature and cause of neurological diseases. Here we show that the number of neurons identified and quantified using this method misses a substantial percentage of extant neurons in a phenotype specific manner. In mice, 24% more hypocretin/orexin (Hcrt) neurons are seen in the night compared to the day, and an additional 17% are seen after inhibiting microtubule polymerization with colchicine. We see no such difference between the number of MCH (melanin concentrating hormone) neurons in dark, light or colchicine conditions, despite MCH and Hcrt both being hypothalamic peptide transmitters. Although the size of Hcrt neurons did not differ between light and dark, the size of MCH neurons was increased by 15% in the light phase. The number of neurons containing histidine decarboxylase (HDC), the histamine synthesizing enzyme, was 34% greater in the dark than in the light, but, like Hcrt, cell size did not differ. We did not find a significant difference in the number or the size of neurons expressing choline acetyltransferase (ChAT), the acetylcholine synthesizing enzyme, in the horizontal diagonal band (HBD) during the dark and light conditions. As expected, colchicine treatment did not increase the number of these neurons. Understanding the function and dynamics of transmitter production within "non-visible" phenotypically defined cells has fundamental implications for our understanding of brain plasticity.
[Mh] Termos MeSH primário: Ritmo Circadiano
Histamina/biossíntese
Neurônios/metabolismo
Orexinas/biossíntese
[Mh] Termos MeSH secundário: Animais
Colina O-Acetiltransferase/metabolismo
Colchicina/administração & dosagem
Histidina Descarboxilase/metabolismo
Hormônios Hipotalâmicos/metabolismo
Masculino
Melaninas/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Neurônios/citologia
Neurônios/enzimologia
Hormônios Hipofisários/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypothalamic Hormones); 0 (Melanins); 0 (Orexins); 0 (Pituitary Hormones); 67382-96-1 (melanin-concentrating hormone); 820484N8I3 (Histamine); EC 2.3.1.6 (Choline O-Acetyltransferase); EC 4.1.1.22 (Histidine Decarboxylase); SML2Y3J35T (Colchicine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178573


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[PMID]:28511597
[Au] Autor:Equihua-Benítez AC; Guzmán-Vásquez K; Drucker-Colín R
[Ad] Endereço:a Departamento de Neuropatología Molecular , Instituto de Fisiología Celular, Universidad Nacional Autónoma de México , Ciudad de México , México.
[Ti] Título:Understanding sleep-wake mechanisms and drug discovery.
[So] Source:Expert Opin Drug Discov;12(7):643-657, 2017 Jul.
[Is] ISSN:1746-045X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Although not discernible at first glance, sleep is a highly active and regulated brain state. Although we spend practically one third of our lifetimes in this stage, its importance is often taken for granted. Sleep loss can lead to disease, error and economic loss. Our understanding of how sleep is achieved has greatly advanced in recent years, and with that, the management of sleep disorders has improved. There is still room for improvement and recently many new compounds have reached clinical trials with a few being approved for commercial use. Areas covered: In this review, the authors make the case of sleep disorders as a matter of public health. The mechanisms of sleep transition are discussed emphasizing the wake and sleep promoting interaction of different brain regions. Finally, advances in pharmacotherapy are examined in the context of chronic insomnia and narcolepsy. Expert opinion: The orexinergic system is an example of a breakthrough in sleep medicine that has catalyzed drug development. Nevertheless, sleep is a topic still with many unanswered questions. That being said, the melanin-concentrating hormone system is becoming increasingly relevant and we speculate it will be the next target of sleep medication.
[Mh] Termos MeSH primário: Descoberta de Drogas/métodos
Transtornos do Sono-Vigília/tratamento farmacológico
Sono/fisiologia
[Mh] Termos MeSH secundário: Animais
Desenho de Drogas
Seres Humanos
Hormônios Hipotalâmicos/metabolismo
Melaninas/metabolismo
Narcolepsia/tratamento farmacológico
Narcolepsia/fisiopatologia
Hormônios Hipofisários/metabolismo
Distúrbios do Início e da Manutenção do Sono/tratamento farmacológico
Distúrbios do Início e da Manutenção do Sono/fisiopatologia
Transtornos do Sono-Vigília/fisiopatologia
Vigília/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Hypothalamic Hormones); 0 (Melanins); 0 (Pituitary Hormones); 67382-96-1 (melanin-concentrating hormone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170518
[St] Status:MEDLINE
[do] DOI:10.1080/17460441.2017.1329818


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[PMID]:28423326
[Au] Autor:Nectow AR; Moya MV; Ekstrand MI; Mousa A; McGuire KL; Sferrazza CE; Field BC; Rabinowitz GS; Sawicka K; Liang Y; Friedman JM; Heintz N; Schmidt EF
[Ad] Endereço:Laboratory of Molecular Genetics, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA; Princeton Neuroscience Institute, Princeton University, Lot 20 Washington Road, Princeton, NJ 08544, USA. Electronic address: anectow@princeton.edu.
[Ti] Título:Rapid Molecular Profiling of Defined Cell Types Using Viral TRAP.
[So] Source:Cell Rep;19(3):655-667, 2017 Apr 18.
[Is] ISSN:2211-1247
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Translational profiling methodologies enable the systematic characterization of cell types in complex tissues, such as the mammalian brain, where neuronal isolation is exceptionally difficult. Here, we report a versatile strategy for profiling CNS cell types in a spatiotemporally restricted fashion by engineering a Cre-dependent adeno-associated virus expressing an EGFP-tagged ribosomal protein (AAV-FLEX-EGFPL10a) to access translating mRNAs by translating ribosome affinity purification (TRAP). We demonstrate the utility of this AAV to target a variety of genetically and anatomically defined neural populations expressing Cre recombinase and illustrate the ability of this viral TRAP (vTRAP) approach to recapitulate the molecular profiles obtained by bacTRAP in corticothalamic neurons across multiple serotypes. Furthermore, spatially restricting adeno-associated virus (AAV) injections enabled the elucidation of regional differences in gene expression within this cell type. Altogether, these results establish the broad applicability of the vTRAP strategy for the molecular dissection of any CNS or peripheral cell type that can be engineered to express Cre.
[Mh] Termos MeSH primário: Cromatografia de Afinidade/métodos
Biossíntese de Proteínas
Ribossomos/metabolismo
Vírus/metabolismo
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Dependovirus/metabolismo
Feminino
Regulação da Expressão Gênica
Proteínas de Fluorescência Verde/metabolismo
Hormônios Hipotalâmicos/metabolismo
Hipotálamo/metabolismo
Masculino
Melaninas/metabolismo
Camundongos
Neurônios/metabolismo
Hormônios Hipofisários/metabolismo
Reprodutibilidade dos Testes
Sorotipagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Hypothalamic Hormones); 0 (Melanins); 0 (Pituitary Hormones); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins); 67382-96-1 (melanin-concentrating hormone)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE


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[PMID]:28324026
[Au] Autor:Qi X; Zhou W; Wang Q; Guo L; Lu D; Lin H
[Ad] Endereço:State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
[Ti] Título:Gonadotropin-Inhibitory Hormone, the Piscine Ortholog of LPXRFa, Participates in 17ß-Estradiol Feedback in Female Goldfish Reproduction.
[So] Source:Endocrinology;158(4):860-873, 2017 04 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gonadotropin-inhibitory hormone (GnIH) plays a critical role in regulating gonadotropin-releasing hormone, gonadotropin hormone, and steroidogenesis in teleosts. In the present study, we sought to determine whether 17ß-estradiol (E2) acts directly on GnIH neurons to regulate reproduction in goldfish, a seasonal breeder, and we investigated the role of estrogen receptors (ERs) in mediating this process. We found that GnIH neurons coexpress three types of ERs. Ovariectomy and letrozole implantation into female goldfish at the vitellogenic stage elicited a substantial decrease in the expression of GnIH messenger RNA (mRNA), and E2 supplementation abolished this effect. In primary cultured hypothalamus cells, E2 increased GnIH mRNA levels; surprisingly, selective ERα and ERß agonists showed opposite effects in regulating GnIH mRNA levels. Using genome walking, we isolated a 2329-bp section of the GnIH promoter sequence, and 7 half-estrogen response elements (EREs) were found in the promoter region. Luciferase assays and electrophoretic mobility shift assay results show that the half-ERE element at -2203 is the key site for competitive binding between ERα and ERß. Ovariectomy and letrozole implantation into female goldfish in the maturating stage did not change the GnIH mRNA expression levels. Taken together, these findings suggest that E2 binds to multiple types of ERs, which competitively bind to the same half-ERE binding site of the GnIH promoter to achieve both positive and negative feedback in response to estrogen to regulate goldfish reproduction at different stages of ovarian development.
[Mh] Termos MeSH primário: Estradiol/metabolismo
Retroalimentação Fisiológica/fisiologia
Hormônios Hipotalâmicos/metabolismo
Hipotálamo/metabolismo
Neurônios/metabolismo
[Mh] Termos MeSH secundário: Animais
Inibidores da Aromatase/farmacologia
Estradiol/farmacologia
Receptor alfa de Estrogênio/metabolismo
Receptor beta de Estrogênio/metabolismo
Retroalimentação Fisiológica/efeitos dos fármacos
Feminino
Carpa Dourada
Hipotálamo/citologia
Hipotálamo/efeitos dos fármacos
Neurônios/efeitos dos fármacos
Nitrilos/farmacologia
Ovariectomia
Reprodução/efeitos dos fármacos
Reprodução/fisiologia
Triazóis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aromatase Inhibitors); 0 (Estrogen Receptor alpha); 0 (Estrogen Receptor beta); 0 (Hypothalamic Hormones); 0 (Nitriles); 0 (Triazoles); 4TI98Z838E (Estradiol); 7LKK855W8I (letrozole)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1210/en.2016-1550


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[PMID]:28322083
[Au] Autor:Gerics B; Szalay F; Sótonyi P; Jancsik V
[Ad] Endereço:Department of Anatomy and Histology, University of Veterinary Medicine , H-1078 Budapest, István utca 2 , Hungary.
[Ti] Título:Diurnal variation of the melanin-concentrating hormone level in the hypothalamus.
[So] Source:Acta Biol Hung;68(1):14-21, 2017 Mar.
[Is] ISSN:0236-5383
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:Melanin-concentrating hormone (MCH), the neuropeptide produced mainly in the hypothalamus, plays an operative role in regulating food intake and the sleep/wake cycle. Considering that these physiological functions pursue diurnal variations, we checked whether the total hypothalamic MCH level depends on the time of the day. The aggregated MCH peptide content of the whole MCH neuron population was significantly higher at the end of the sleeping period (lights on), than at the end of the active period (lights off). This result, together with earlier observations, indicates that in contrast to the MCH gene expression, the level of MCH peptide is object of circadian variation in the hypothalamus.
[Mh] Termos MeSH primário: Ritmo Circadiano
Hormônios Hipotalâmicos/metabolismo
Hipotálamo/metabolismo
Melaninas/metabolismo
Hormônios Hipofisários/metabolismo
[Mh] Termos MeSH secundário: Animais
Hipotálamo/citologia
Imuno-Histoquímica
Masculino
Camundongos Endogâmicos
Neurônios/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypothalamic Hormones); 0 (Melanins); 0 (Pituitary Hormones); 67382-96-1 (melanin-concentrating hormone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1556/018.68.2017.1.2


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[PMID]:28300612
[Au] Autor:Cui L; Lv C; Zhang J; Mo C; Lin D; Li J; Wang Y
[Ad] Endereço:Key laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, PR China.
[Ti] Título:Characterization of melanin-concentrating hormone (MCH) and its receptor in chickens: Tissue expression, functional analysis, and fasting-induced up-regulation of hypothalamic MCH expression.
[So] Source:Gene;615:57-67, 2017 Jun 05.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Melanin-concentrating hormone (MCH) is a neuropeptide expressed in the brain and exerts its actions through interaction with the two known G protein-coupled receptors, namely melanin-concentrating hormone receptor 1 and 2 (MCHR1 and MCHR2) in mammals. However, the information regarding the expression and functionality of MCH and MCHR(s) remains largely unknown in birds. In this study, using RT-PCR and RACE PCR, we amplified and cloned a MCHR1-like receptor, which is named cMCHR4 according to its evolutionary origin, and a MCHR2 from chicken brain. The cloned cMCHR4 was predicted to encode a receptor of 367 amino acids, which shares high amino acid identities with MCHR4 of ducks (90%), western painted turtles (85%), and coelacanths (77%), and a comparatively low identity to human MCHR1 (58%) and MCHR2 (38%), whereas chicken MCHR2 encodes a putative C-terminally truncated receptor and is likely a pseudogene. Using cell-based luciferase reporter assays or Western blot, we further demonstrated that chicken (and duck) MCHR4 could be potently activated by chicken MCH , and its activation can elevate calcium concentration and activate MAPK/ERK and cAMP/PKA signaling pathways, indicating an important role of MCHR4 in mediating MCH actions in birds. Quantitative real-time PCR revealed that both cMCH and cMCHR4 mRNA are expressed in various brain regions including the hypothalamus, and cMCH expression in the hypothalamus of 3-week-old chicks could be induced by 36-h fasting, indicating that cMCH expression is correlated with energy balance. Taken together, characterization of chicken MCH and MCHR4 will aid to uncover the conserved roles of MCH across vertebrates.
[Mh] Termos MeSH primário: Galinhas/genética
Hormônios Hipotalâmicos/genética
Hipotálamo/metabolismo
Melaninas/genética
Hormônios Hipofisários/genética
Receptores do Hormônio Hipofisário/genética
[Mh] Termos MeSH secundário: Animais
Clonagem Molecular
Patos/genética
Jejum
Regulação da Expressão Gênica
Células HEK293
Seres Humanos
Hormônios Hipotalâmicos/metabolismo
Melaninas/metabolismo
Hormônios Hipofisários/metabolismo
Receptores do Hormônio Hipofisário/metabolismo
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypothalamic Hormones); 0 (Melanins); 0 (Pituitary Hormones); 0 (Receptors, Pituitary Hormone); 0 (melanin-concentrating hormone receptor); 67382-96-1 (melanin-concentrating hormone)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170413
[Lr] Data última revisão:
170413
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE


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[PMID]:28236845
[Au] Autor:McCloskey RJ
[Ad] Endereço:University of Pennsylvania, United States. Electronic address: rmcclosk@gmail.com.
[Ti] Título:Sleep and cargo reorganization: A hypothesis.
[So] Source:Med Hypotheses;100:37-42, 2017 Mar.
[Is] ISSN:1532-2777
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Several molecules that act in the nervous system to regulate sleep and wake were first identified based on their transport effects in pigmented cells. I compiled a list of such molecules like melatonin, melanin-concentrating hormone, and pigment dispersing factor, etc. Molecules that induce pigment aggregation promote sleep whereas molecules that induce pigment dispersal promote wake. I call these Sleep and PIgment Regulating Factors SPIRFs. SPIRFs regulate organelle trafficking in both pigmentary models and neurons. I propose that cargo transport fulfills necessary sleep functions such as remodeling synapses and restoring homeostasis in the distribution of cell components. I put forth the hypothesis that sleep-promoting SPIRFs induce states of increased cargo movement towards the cell body, and propose that this function is a critical neuron maintenance task for which animals must sleep.
[Mh] Termos MeSH primário: Organelas/metabolismo
Sono/fisiologia
[Mh] Termos MeSH secundário: Animais
Homeostase/fisiologia
Seres Humanos
Hormônios Hipotalâmicos/metabolismo
Melaninas/metabolismo
Melatonina/metabolismo
Modelos Neurológicos
Modelos Teóricos
Neurônios/metabolismo
Neurônios/fisiologia
Pigmentação
Hormônios Hipofisários/metabolismo
Ratos
Vigília
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Hypothalamic Hormones); 0 (Melanins); 0 (Pituitary Hormones); 67382-96-1 (melanin-concentrating hormone); JL5DK93RCL (Melatonin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE



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