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[PMID]: | 28974369 |
[Au] Autor: | Ávila-Mendoza J; Pérez-Rueda E; Urban-Sosa V; Carranza M; Martínez-Moreno CG; Luna M; Arámburo C |
[Ad] Endereço: | Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro, Qro. 76230, Mexico. |
[Ti] Título: | Characterization and distribution of GHRH, PACAP, TRH, SST and IGF1 mRNAs in the green iguana. |
[So] Source: | Gen Comp Endocrinol;255:90-101, 2018 Jan 01. | [Is] ISSN: | 1095-6840 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | The somatotropic axis (SA) regulates numerous aspects of vertebrate physiology such as development, growth, and metabolism and has influence on several tissues including neural, immune, reproductive and gastric tract. Growth hormone (GH) is a key component of SA, it is synthesized and released mainly by pituitary somatotrophs, although now it is known that virtually all tissues can express GH, which, in addition to its well-described endocrine roles, also has autocrine/paracrine/intracrine actions. In the pituitary, GH expression is regulated by several hypothalamic neuropeptides including GHRH, PACAP, TRH and SST. GH, in turn, regulates IGF1 synthesis in several target tissues, adding complexity to the system since GH effects can be exerted either directly or mediated by IGF1. In reptiles, little is known about the SA components and their functional interactions. The aim of this work was to characterize the mRNAs of the principal SA components in the green iguana and to develop the tools that allow the study of the structural and functional evolution of this system in reptiles. By employing RT-PCR and RACE, the cDNAs encoding for GHRH, PACAP, TRH, SST and IGF1 were amplified and sequenced. Results showed that these cDNAs coded for the corresponding protein precursors of 154, 170, 243, 113, and 131 amino acids, respectively. Of these, GHRH, PACAP, SST and IGF1 precursors exhibited a high structural conservation with respect to its counterparts in other vertebrates. On the other hand, iguana's TRH precursor showed 7 functional copies of mature TRH (pyr-QHP-NH ), as compared to 4 and 6 copies of TRH in avian and mammalian proTRH sequences, respectively. It was found that in addition to its primary production site (brain for GHRH, PACAP, TRH and SST, and liver for IGF1), they were also expressed in other peripheral tissues, i.e. testes and ovaries expressed all the studied mRNAs, whereas TRH and IGF1 mRNAs were observed ubiquitously in all tissues considered. These results show that the main SA components in reptiles of the Squamata Order maintain a good structural conservation among vertebrate phylogeny, and suggest important physiological interactions (endocrine, autocrine and/or paracrine) between them due to their wide peripheral tissue expression. |
[Mh] Termos MeSH primário: |
Hormônio Liberador de Hormônio do Crescimento/genética Iguanas/genética Fator de Crescimento Insulin-Like I/genética Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/genética Somatostatina/genética Hormônio Liberador de Tireotropina/genética
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[Mh] Termos MeSH secundário: |
Sequência de Aminoácidos Animais Sequência de Bases Hormônio Liberador de Hormônio do Crescimento/química Hormônio Liberador de Hormônio do Crescimento/metabolismo Fator de Crescimento Insulin-Like I/química Fator de Crescimento Insulin-Like I/metabolismo Filogenia Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/química Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/metabolismo RNA Mensageiro/genética RNA Mensageiro/metabolismo Somatostatina/química Somatostatina/metabolismo Hormônio Liberador de Tireotropina/química Hormônio Liberador de Tireotropina/metabolismo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Pituitary Adenylate Cyclase-Activating Polypeptide); 0 (RNA, Messenger); 51110-01-1 (Somatostatin); 5Y5F15120W (Thyrotropin-Releasing Hormone); 67763-96-6 (Insulin-Like Growth Factor I); 9034-39-3 (Growth Hormone-Releasing Hormone) |
[Em] Mês de entrada: | 1802 |
[Cu] Atualização por classe: | 180216 |
[Lr] Data última revisão:
| 180216 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171005 |
[St] Status: | MEDLINE |
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