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[PMID]: 28820912 [Au] Autor: Prazienková V; Holubová M; Pelantová H; Bugánová M; Pirník Z; Mikulásková B; Popelová A; Blechová M; Haluzík M; Zelezná B; Kuzma M; Kunes J; Maletínská L [Ad] Endereço: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic. [Ti] Título: Impact of novel palmitoylated prolactin-releasing peptide analogs on metabolic changes in mice with diet-induced obesity. [So] Source: PLoS One;12(8):e0183449, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Analogs of anorexigenic neuropeptides, such as prolactin-releasing peptide (PrRP), have a potential as new anti-obesity drugs. In our previous study, palmitic acid attached to the N-terminus of PrRP enabled its central anorexigenic effects after peripheral administration. In this study, two linkers, γ-glutamic acid at Lys11 and a short, modified polyethylene glycol at the N-terminal Ser and/or Lys11, were applied for the palmitoylation of PrRP31 to improve its bioavailability. These analogs had a high affinity and activation ability to the PrRP receptor GPR10 and the neuropeptide FF2 receptor, as well as short-term anorexigenic effect similar to PrRP palmitoylated at the N-terminus. Two-week treatment with analogs that were palmitoylated through linkers to Lys11 (analogs 1 and 2), but not with analog modified both at the N-terminus and Lys11 (analog 3) decreased body and liver weights, insulin, leptin, triglyceride, cholesterol and free fatty acid plasma levels in a mouse model of diet-induced obesity. Moreover, the expression of uncoupling protein-1 was increased in brown fat suggesting an increase in energy expenditure. In addition, treatment with analogs 1 and 2 but not analog 3 significantly decreased urinary concentrations of 1-methylnicotinamide and its oxidation products N-methyl-2-pyridone-5-carboxamide and N-methyl-4-pyridone-3-carboxamide, as shown by NMR-based metabolomics. This observation confirmed the previously reported increase in nicotinamide derivatives in obesity and type 2 diabetes mellitus and the effectiveness of analogs 1 and 2 in the treatment of these disorders. [Mh] Termos MeSH primário: Dieta Obesidade/metabolismo Peptídeos/farmacologia Hormônio Liberador de Prolactina/metabolismo [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Ligação Competitiva Células CHO Cricetinae Cricetulus Masculino Metabolômica Camundongos Camundongos Endogâmicos C57BL Ressonância Magnética Nuclear Biomolecular Obesidade/etiologia Peptídeos/química Hormônio Liberador de Prolactina/química beta-Lactamases/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Peptides); 0 (Prolactin-Releasing Hormone); EC 3.5.2.6 (beta-Lactamases) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171013 [Lr] Data última revisão: 171013 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170819 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0183449

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[PMID]: 27495953 [Au] Autor: Russart KL; Rhen T [Ad] Endereço: Department of Biology, University of North Dakota, 10 Cornell Street Stop 9019, Starcher Hall Room 100, Grand Forks, ND, 58202, United States of America. Electronic address: kathryn.gruchalla@und.edu. [Ti] Título: Atrazine alters expression of reproductive and stress genes in the developing hypothalamus of the snapping turtle, Chelydra serpentina. [So] Source: Toxicology;366-367:1-9, 2016 Jul 29. [Is] ISSN: 1879-3185 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: Atrazine is an herbicide used to control broadleaf grasses and a suspected endocrine disrupting chemical. Snapping turtles lay eggs between late May and early June, which could lead to atrazine exposure via field runoff. Our goal was to determine whether a single exposure to 2ppb or 40ppb atrazine during embryogenesis could induce short- and long-term changes in gene expression within the hypothalamus of snapping turtles. We treated eggs with atrazine following sex determination and measured gene expression within the hypothalamus. We selected genes a priori for their role in the hypothalamus-pituitary-gonad or the hypothalamus-pituitary-adrenal axes of the endocrine system. We did not identify any changes in gene expression 24-h after treatment. However, at hatching AR, Kiss1R, and POMC expression was upregulated in both sexes, while expression of CYP19A1 and PDYN was increased in females. Six months after hatching, CYP19A1 and PRLH expression was increased in animals treated with 2ppb atrazine. Our study shows persistent changes in hypothalamic gene expression due to low-dose embryonic exposure to the herbicide atrazine with significant effects in both the HPG and HPA axes. Effects reported here appear to be conserved among vertebrates. [Mh] Termos MeSH primário: Atrazina/toxicidade Herbicidas/toxicidade Hipotálamo/efeitos dos fármacos Organogênese/efeitos dos fármacos Reprodução/efeitos dos fármacos [Mh] Termos MeSH secundário: Animais Aromatase/genética Aromatase/metabolismo Relação Dose-Resposta a Droga Encefalinas/genética Encefalinas/metabolismo Feminino Regulação da Expressão Gênica Sistema Hipotálamo-Hipofisário/efeitos dos fármacos Sistema Hipotálamo-Hipofisário/metabolismo Hipotálamo/metabolismo Masculino Hipófise/efeitos dos fármacos Hipófise/metabolismo Hormônio Liberador de Prolactina/genética Hormônio Liberador de Prolactina/metabolismo Precursores de Proteínas/genética Precursores de Proteínas/metabolismo Fatores Sexuais Tartarugas/embriologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Enkephalins); 0 (Herbicides); 0 (Prolactin-Releasing Hormone); 0 (Protein Precursors); 93443-35-7 (preproenkephalin); EC 1.14.14.1 (Aromatase); QJA9M5H4IM (Atrazine) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170512 [Lr] Data última revisão: 170512 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160807 [St] Status: MEDLINE

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[PMID]: 27418033 [Au] Autor: Kunes J; Prazienková V; Popelová A; Mikulásková B; Zemenová J; Maletínská L [Ad] Endereço: Institute of Organic Chemistry and BiochemistryAcademy of Sciences of the Czech Republic, Prague, Czech Republic Institute of PhysiologyAcademy of Sciences of the Czech Republic, Prague, Czech Republic. [Ti] Título: Prolactin-releasing peptide: a new tool for obesity treatment. [So] Source: J Endocrinol;230(2):R51-8, 2016 Aug. [Is] ISSN: 1479-6805 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Obesity is an escalating epidemic, but an effective noninvasive therapy is still scarce. For obesity treatment, anorexigenic neuropeptides are promising tools, but their delivery from the periphery to the brain is complicated because peptides have a low stability and limited ability to cross the blood-brain barrier. In this review, we summarize results of several studies with our newly designed lipidized analogs of prolactin-releasing peptide (PrRP). PrRP is involved in feeding and energy balance regulation as demonstrated by obesity phenotypes of both PrRP- and PrRP-receptor-knockout mice. Lipidized PrRP analogs showed binding affinity and signaling in PrRP receptor-expressing cells similar to natural PrRP. Moreover, these analogs showed high binding affinity also to anorexigenic neuropeptide FF (NPFF)-2 receptor. Acute peripheral administration of myristoylated and palmitoylated PrRP analogs to mice and rats induced strong and long-lasting anorexigenic effects and neuronal activation in the brain areas involved in food intake regulation. Two-week-long subcutaneous administration of palmitoylated PrRP31 and myristoylated PrRP20 lowered food intake, body weight, improved metabolic parameters and attenuated lipogenesis in mice with diet-induced obesity. A strong anorexigenic, body weight-reducing and glucose tolerance-improving effect of palmitoylated-PrRP31 was shown also in diet-induced obese rats after its repeated 2-week-long peripheral administration. Thus, the strong anorexigenic and body weight-reducing effects of palmitoylated PrRP31 and myristoylated PrRP20 make these analogs attractive candidates for antiobesity treatment. Moreover, PrRP receptor might be a new target for obesity therapy. [Mh] Termos MeSH primário: Fármacos Antiobesidade/uso terapêutico Regulação do Apetite Sistemas de Liberação de Medicamentos Obesidade/tratamento farmacológico Hormônio Liberador de Prolactina/uso terapêutico [Mh] Termos MeSH secundário: Animais Fármacos Antiobesidade/farmacologia Seres Humanos Hormônio Liberador de Prolactina/análogos & derivados [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Anti-Obesity Agents); 0 (Prolactin-Releasing Hormone) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170517 [Lr] Data última revisão: 170517 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160716 [St] Status: MEDLINE [do] DOI: 10.1530/JOE-16-0046

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[PMID]: 27010901 [Au] Autor: Prazienkova V; Ticha A; Blechova M; Spolcova A; Zelezna B; Maletinska L [Ad] Endereço: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic. [Ti] Título: Pharmacological characterization of lipidized analogs of prolactin-releasing peptide with a modified C- terminal aromatic ring. [So] Source: J Physiol Pharmacol;67(1):121-8, 2016 Feb. [Is] ISSN: 1899-1505 [Cp] País de publicação: Poland [La] Idioma: eng [Ab] Resumo: Prolactin-releasing peptide (PrRP) is an anorexigenic neuropeptide expressed in the brain where it regulates food intake and energy expenditure. The C-terminal Arg-Phe-NH2 of PrRP is crucial for its biological activity. In our previous study, we showed that PrRP analogs myristoylated or palmitoylated at the N- terminus seem to cross the blood-brain barrier and lower food intake following peripheral administration. In this study, myristoylated and palmitoylated PrRP31 analogs with a modified C-terminal Phe were designed and tested. Lipidized analogs containing Phe(31) replaced by aromatic non-coded amino acids or tyrosine revealed high binding affinity to rat pituitary RC-4B/C cells with endogenous PrRP and neuropeptide FF 2 receptors and to CHO-K1 cells overexpressing either PrRP or neuropeptide FF 2 receptors. The analogs also showed strong agonistic properties at the GPR10 receptor using the beta-lactamase reporter gene assay. Moreover, lipidized PrRP analogs, especially those that were palmitoylated, demonstrated strong and long-lasting anorexigenic effects in fasted mice after subcutaneous administration. The most efficient PrRP31 analogs with PheCl2(31), either palmitoylated or myristoylated at the N-terminus, are promising candidates for the study of food disorders, possibly for anti-obesity treatment. Despite the therapeutic potential in targeting central GPR10, the endogenous ligand PrRP cannot cross the blood-brain barrier. Understanding biological activity and transport of novel structural analogs of PrRP with a potential central anorexigenic effect is of key therapeutic significance. [Mh] Termos MeSH primário: Neuropeptídeos/farmacologia Hormônio Liberador de Prolactina/análogos & derivados Hormônio Liberador de Prolactina/farmacologia [Mh] Termos MeSH secundário: Animais Barreira Hematoencefálica/metabolismo Encéfalo/metabolismo Células CHO Linhagem Celular Cricetulus Ingestão de Alimentos/efeitos dos fármacos Seres Humanos Lipídeos Masculino Camundongos Camundongos Endogâmicos C57BL Neuropeptídeos/metabolismo Obesidade/tratamento farmacológico Obesidade/metabolismo Hormônio Liberador de Prolactina/metabolismo Ratos Receptores de Neuropeptídeos/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Lipids); 0 (Neuropeptides); 0 (Prolactin-Releasing Hormone); 0 (Receptors, Neuropeptide); 0 (neuropeptide FF receptor) [Em] Mês de entrada: 1612 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160325 [St] Status: MEDLINE

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[PMID]: 26918308 [Au] Autor: Liutkus M; Fraser SA; Caron K; Stigers DJ; Easton CJ [Ad] Endereço: Research School of Chemistry, Australian National University, Canberra, ACT, 2601, Australia. [Ti] Título: Peptide Synthesis through Cell-Free Expression of Fusion Proteins Incorporating Modified Amino Acids as Latent Cleavage Sites for Peptide Release. [So] Source: Chembiochem;17(10):908-12, 2016 May 17. [Is] ISSN: 1439-7633 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Chlorinated analogues of Leu and Ile are incorporated during cell-free expression of peptides fused to protein, by exploiting the promiscuity of the natural biosynthetic machinery. They then act as sites for clean and efficient release of the peptides simply by brief heat treatment. Dehydro analogues of Leu and Ile are similarly incorporated as latent sites for peptide release through treatment with iodine under cold conditions. These protocols complement enzyme-catalyzed methods and have been used to prepare calcitonin, gastrin-releasing peptide, cholecystokinin-7, and prolactin-releasing peptide prohormones, as well as analogues substituted with unusual amino acids, thus illustrating their practical utility as alternatives to more traditional chemical peptide synthesis. [Mh] Termos MeSH primário: Aminoácidos/metabolismo Técnicas de Química Sintética/métodos Peptídeos/metabolismo [Mh] Termos MeSH secundário: Sequência de Aminoácidos Calcitonina/química Calcitonina/metabolismo Proteínas de Transporte/genética Proteínas de Transporte/metabolismo Sistema Livre de Células Peptídeo Liberador de Gastrina/química Peptídeo Liberador de Gastrina/metabolismo Dados de Sequência Molecular Peptídeos/química Hormônio Liberador de Prolactina/química Hormônio Liberador de Prolactina/metabolismo Proteínas Recombinantes de Fusão/biossíntese Proteínas Recombinantes de Fusão/química Proteínas Recombinantes de Fusão/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Amino Acids); 0 (Carrier Proteins); 0 (Peptides); 0 (Prolactin-Releasing Hormone); 0 (Recombinant Fusion Proteins); 80043-53-4 (Gastrin-Releasing Peptide); 9007-12-9 (Calcitonin) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170328 [Lr] Data última revisão: 170328 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160227 [St] Status: MEDLINE [do] DOI: 10.1002/cbic.201600091

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[PMID]: 26906745 [Au] Autor: Holubová M; Zemenová J; Mikulásková B; Panajotova V; Stöhr J; Haluzík M; Kunes J; Zelezná B; Maletínská L [Ad] Endereço: Institute of Organic Chemistry and BiochemistryAcademy of Sciences of the Czech Republic, Prague, Czech Republic. [Ti] Título: Palmitoylated PrRP analog decreases body weight in DIO rats but not in ZDF rats. [So] Source: J Endocrinol;229(2):85-96, 2016 May. [Is] ISSN: 1479-6805 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Anorexigenic neuropeptides produced and acting in the brain have the potential to decrease food intake and ameliorate obesity, but are ineffective after peripheral application, owing to a limited ability to cross the blood-brain barrier. We have designed lipidized analogs of prolactin-releasing peptide (PrRP), which is involved in energy balance regulation as demonstrated by obesity phenotypes of both Prrp-knockout and Prrp receptor-knockout mice. The aim of this study was to characterize the subchronic effect of a palmitoylated PrRP analog in two rat models of obesity and diabetes: diet-induced obese Sprague-Dawley rats and leptin receptor-deficient Zucker diabetic (ZDF) rats. In the rats with diet-induced obesity (DIO), a two-week intraperitoneal treatment with palmitoylated PrRP lowered food intake by 24% and body weight by 8%. This treatment also improved glucose tolerance and tended to decrease leptin levels and adipose tissue masses in a dose-dependent manner. In contrast, in ZDF rats, the same treatment with palmitoylated PrRP lowered food intake but did not significantly affect body weight or glucose tolerance, probably in consequence of severe leptin resistance due to a nonfunctional leptin receptor. Our data indicate a good efficacy of lipidized PrRP in DIO rats. Thus, the strong anorexigenic, body weight-reducing, and glucose tolerance-improving effects make palmitoylated PrRP an attractive candidate for anti-obesity treatment. [Mh] Termos MeSH primário: Peso Corporal/efeitos dos fármacos Obesidade/tratamento farmacológico Hormônio Liberador de Prolactina/análogos & derivados [Mh] Termos MeSH secundário: Tecido Adiposo/efeitos dos fármacos Tecido Adiposo/metabolismo Animais Depressores do Apetite/farmacologia Diabetes Mellitus Experimental/metabolismo Dieta Hiperlipídica/efeitos adversos Desenho de Drogas Ingestão de Alimentos/efeitos dos fármacos Teste de Tolerância a Glucose Metabolismo dos Lipídeos/efeitos dos fármacos Metabolismo dos Lipídeos/genética Lipoilação Fígado/efeitos dos fármacos Fígado/metabolismo Masculino Camundongos Obesidade/metabolismo Obesidade/patologia Hormônio Liberador de Prolactina/farmacologia RNA Mensageiro/genética RNA Mensageiro/metabolismo Ratos Ratos Sprague-Dawley Ratos Zucker Receptores para Leptina/deficiência [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Appetite Depressants); 0 (Prolactin-Releasing Hormone); 0 (RNA, Messenger); 0 (Receptors, Leptin) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170608 [Lr] Data última revisão: 170608 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160225 [St] Status: MEDLINE [do] DOI: 10.1530/JOE-15-0519

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[PMID]: 26643957 [Au] Autor: Mikulásková B; Zemenová J; Pirník Z; Prazienková V; Bednárová L; Zelezná B; Maletínská L; Kunes J [Ad] Endereço: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Czech Republic; Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. [Ti] Título: Effect of palmitoylated prolactin-releasing peptide on food intake and neural activation after different routes of peripheral administration in rats. [So] Source: Peptides;75:109-17, 2016 Jan. [Is] ISSN: 1873-5169 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Obesity is an escalating epidemic, but an effective non-invasive therapy is still scarce. For obesity treatment, anorexigenic neuropeptides are promising tools, but their delivery from the periphery to the brain is complicated by their peptide character. In order to overcome this unfavorable fact, we have applied the lipidization of neuropeptide prolactin-releasing peptide (PrRP), whose strong anorexigenic effect was demonstrated. A palmitoylated analog of human PrRP (h palm-PrRP31) was injected in free-fed Wistar rats by three routes: subcutaneous (s.c.), intraperitoneal (i.p) (both 5 mg/kg) and intravenous (i.v.) (from 0.01 to 0.5 mg/kg). We found a circulating compound in the blood after all three applications with the highest concentration after i.v. administration. This corresponds to the effect on food intake, which was also strongest after i.v. injection. Moreover, this is in agreement with the fact that the expression of c-Fos in specific brain regions involved in food intake regulation was also highest after intravenous application. Pharmacokinetic data are further supported by results obtained from dynamic light scattering and CD spectroscopy. Human palm-PrRP31 analog showed a strong tendency to micellize, and formation of aggregates suggested lower availability after i.p. or s.c. application. We have demonstrated that palm-PrRP influenced food intake even in free fed rats. Not surprisingly, the maximal effect was achieved after the intravenous application even though two orders of magnitude lower dose was used compared to both two other applications. We believe that palm-PrRP could have a potential as an antiobesity drug when its s.c. application would be improved. [Mh] Termos MeSH primário: Fármacos Antiobesidade/administração & dosagem Ingestão de Energia/efeitos dos fármacos Fragmentos de Peptídeos/administração & dosagem Hormônio Liberador de Prolactina/análogos & derivados [Mh] Termos MeSH secundário: Animais Fármacos Antiobesidade/metabolismo Fármacos Antiobesidade/farmacocinética Encéfalo/metabolismo Células CHO Cricetinae Cricetulus Avaliação Pré-Clínica de Medicamentos Seres Humanos Neurônios/efeitos dos fármacos Neurônios/metabolismo Obesidade/tratamento farmacológico Fragmentos de Peptídeos/metabolismo Fragmentos de Peptídeos/farmacocinética Hormônio Liberador de Prolactina/administração & dosagem Hormônio Liberador de Prolactina/metabolismo Hormônio Liberador de Prolactina/farmacocinética Ligação Proteica Proteínas Proto-Oncogênicas c-fos/metabolismo Ratos Receptores Acoplados a Proteínas-G/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Obesity Agents); 0 (PRLHR protein, human); 0 (Peptide Fragments); 0 (Prolactin-Releasing Hormone); 0 (Proto-Oncogene Proteins c-fos); 0 (Receptors, G-Protein-Coupled); 0 (palmitoyl-PrRP31 peptide, human) [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151209 [St] Status: MEDLINE

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[PMID]: 26130238 [Au] Autor: Osugi T; Son YL; Ubuka T; Satake H; Tsutsui K [Ad] Endereço: Bioorganic Research Institute, Suntory Foundation for Life Sciences, 1-1-1 Wakayamadai, Shimamoto, Mishima, Osaka 618-8503, Japan. Electronic address: osugi@sunbor.or.jp. [Ti] Título: RFamide peptides in agnathans and basal chordates. [So] Source: Gen Comp Endocrinol;227:94-100, 2016 Feb 01. [Is] ISSN: 1095-6840 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Since a peptide with a C-terminal Arg-Phe-NH2 (RFamide peptide) was first identified in the ganglia of the venus clam in 1977, RFamide peptides have been found in the nervous system of both invertebrates and vertebrates. In vertebrates, the RFamide peptide family includes gonadotropin-inhibitory hormone (GnIH), neuropeptide FF (NPFF), prolactin-releasing peptide (PrRP), pyroglutamylated RFamide peptide/26RFamide peptide (QRFP/26RFa), and kisspeptins (kiss1 and kiss2). They are involved in important functions such as the release of hormones, regulation of sexual or social behavior, pain transmission, reproduction, and feeding. In contrast to tetrapods and jawed fish, the information available on RFamide peptides in agnathans and basal chordates is limited, thus preventing further insights into the evolution of RFamide peptides in vertebrates. In this review, we focus on the previous research and recent advances in the studies on RFamide peptides in agnathans and basal chordates. In agnathans, the genes encoding GnIH, NPFF, and PrRP precursors and the mature peptides have been identified in lamprey (Petromyzon marinus) and hagfish (Paramyxine atami). Putative kiss1 and kiss2 genes have also been found in the genome database of lamprey. In basal chordates, namely, in amphioxus (Branchiostoma japonicum), a common ancestral form of GnIH and NPFF genes and their mature peptides, as well as the ortholog of the QRFP gene have been identified. The studies revealed that the number of orthologs of vertebrate RFamide peptides present in agnathans and basal chordates is greater than expected, suggesting that the vertebrate RFamide peptides might have emerged and expanded at an early stage of chordate evolution. [Mh] Termos MeSH primário: Feiticeiras (Peixe)/metabolismo Kisspeptinas/metabolismo Anfioxos/metabolismo Neuropeptídeos/metabolismo Petromyzon/metabolismo [Mh] Termos MeSH secundário: Animais Evolução Biológica Feiticeiras (Peixe)/genética Kisspeptinas/genética Anfioxos/genética Neuropeptídeos/genética Oligopeptídeos/genética Oligopeptídeos/metabolismo Petromyzon/genética Hormônio Liberador de Prolactina/genética Hormônio Liberador de Prolactina/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW [Nm] Nome de substância: 0 (Kisspeptins); 0 (Neuropeptides); 0 (Oligopeptides); 0 (Prolactin-Releasing Hormone); 0 (RFamide peptide); 99566-27-5 (phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide) [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150702 [St] Status: MEDLINE

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[PMID]: 26362395 [Au] Autor: Pirnik Z; Zelezná B; Kiss A; Maletínská L [Ad] Endereço: Institute of Organic Chemistry and Biochemistry, AS CR, Prague, Czech Republic; Laboratory of Functional Neuromorphology, Institute of Experimental Endocrinology, SAS, Bratislava, Slovak Republic; Department of Human and Clinical Pharmacology, University of Veterinary Medicine, Kosice, Slovak Republ [Ti] Título: Peripheral administration of palmitoylated prolactin-releasing peptide induces Fos expression in hypothalamic neurons involved in energy homeostasis in NMRI male mice. [So] Source: Brain Res;1625:151-8, 2015 Nov 02. [Is] ISSN: 1872-6240 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Energy homeostasis is the result of a balance between energy intake and expenditure, and the hypothalamus plays a key role in the regulation of these processes. The hypothalamic prolactin-releasing peptide (PrRP) is involved in food intake regulation and energy homeostasis, although only its lipidized analogs exert central anorexigenic effects after peripheral administration. The aim of the present study was to delineate the extent of the Fos expression as a marker of neuronal activation within the hypothalamic structures involved in food intake regulation after peripherally administered palmitoylated PrRP31 (palm-PrRP31) and to determine whether the anorexigenic effect of peripherally administered palm-PrRP31 influence the activity of hypocretin (HCRT) and oxytocin (OXY) neurons, i.e., the neuropeptides crucially involved in the regulation of energy homeostasis. The data confirmed an anorexigenic effect of palm-PrRP31 treatment (5mg/kg, s.c.) in mice. In the palm-PrRP31-treated animals, a significant increase in Fos expression was observed in the hypothalamic paraventricular (PVN), dorsomedial (DMN), and arcuate (Arc) nuclei and in the neurons of the nucleus of the solitary tract (NTS). Moreover, significant Fos expression was observed in the lateral hypothalamic area (LHA) HCRT neurons and PVN OXY neurons after palm-PrRP31 administration. The present findings may indicate that palm-PrRP31 may be involved in energy homeostasis via the activation of several hypothalamic structures. Fos activation of the hypothalamic OXY and HCRT neurons in the PVN and LHA emphasizes the importance of the areas mentioned in the central action of palm-PrRP31. [Mh] Termos MeSH primário: Metabolismo Energético/efeitos dos fármacos Hipotálamo/citologia Neurônios/efeitos dos fármacos Proteínas Oncogênicas v-fos/metabolismo Hormônio Liberador de Prolactina/farmacologia [Mh] Termos MeSH secundário: Análise de Variância Animais Ingestão de Alimentos/efeitos dos fármacos Hipotálamo/efeitos dos fármacos Masculino Camundongos Orexinas/metabolismo Ocitocina/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Hcrt protein, mouse); 0 (Oncogene Proteins v-fos); 0 (Orexins); 0 (Prolactin-Releasing Hormone); 50-56-6 (Oxytocin) [Em] Mês de entrada: 1608 [Cu] Atualização por classe: 151107 [Lr] Data última revisão: 151107 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150913 [St] Status: MEDLINE

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MEDLINE

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[PMID]: 26355003 [Au] Autor: Wang G; Tachibana T; Gilbert ER; Cline MA [Ad] Endereço: Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA; and. [Ti] Título: Dietary Macronutrient Composition Affects the Influence of Exogenous Prolactin-Releasing Peptide on Appetite Responses and Hypothalamic Gene Expression in Chickens. [So] Source: J Nutr;145(10):2406-11, 2015 Oct. [Is] ISSN: 1541-6100 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: The interaction between the effects of exogenous neurotransmitters and dietary composition on appetite regulation in nonmammalian species is unclear. OBJECTIVE: The objective of this study was to determine the effects of exogenous prolactin-releasing peptide (PrRP) and dietary macronutrient composition on food intake regulation in broiler chicks. METHODS: Three isocaloric diets were formulated: high-carbohydrate (HC), high-fat (HF; 60% of ME from lard) and high-protein (HP) diets. In Expt. 1, 4-d-old Hubbard × Cobb-500 chicks fed 1 of the 3 diets since hatch were intracerebroventricularly injected with 0 (vehicle), 3, or 188 pmol PrRP (n = 10). Food intake was measured for 180 min. In Expt. 2, hypothalamic mRNA abundance of appetite-associated factors was measured in hypothalamus samples obtained 1 h postinjection of 0 or 188 pmol PrRP. In Expt. 3, chicks were given free access to all diets before and after intracerebroventricular injection and food intake was measured. RESULTS: Three and 188 pmol PrRP increased (P = 0.0008 and 0.04) HP diet intake, but only 188 pmol PrRP was efficacious at increasing HC (P = 0.0011) and HF (P = 0.01) consumption compared with the vehicle. There was a diet effect on mRNA abundance of all genes (P < 0.05), with greater expression in chicks fed the HF or HP than the HC diet. Whereas neuropeptide Y (NPY) mRNA was similar between vehicle- and PrRP-injected chicks that consumed HP or HF diets, expression was greater (P < 0.05) in PrRP- than vehicle-injected chicks that consumed the HC diet. When chicks had access to all diets, 188 pmol PrRP caused preferential (P < 0.0001) intake of the HP over the HC and HF diets. CONCLUSION: The HP diet enhanced the sensitivity of chicks to the food intake-stimulating effects of PrRP, and PrRP in turn increased preference for the HP diet. Thus, dietary macronutrient composition influences PrRP-mediated food intake, and PrRP in turn affects nutrient intake and transcriptional regulation in chicks. [Mh] Termos MeSH primário: Regulação do Apetite/efeitos dos fármacos Estimulantes do Apetite/farmacologia Proteínas na Dieta/administração & dosagem Interações Alimento-Droga Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos Hipotálamo/efeitos dos fármacos Hormônio Liberador de Prolactina/farmacologia [Mh] Termos MeSH secundário: Animais Estimulantes do Apetite/administração & dosagem Galinhas Cruzamentos Genéticos Carboidratos da Dieta/administração & dosagem Relação Dose-Resposta a Droga Ingestão de Energia/efeitos dos fármacos Preferências Alimentares Hipotálamo/crescimento & desenvolvimento Hipotálamo/metabolismo Injeções Intraventriculares Neurônios/efeitos dos fármacos Neurônios/metabolismo Neuropeptídeo Y/agonistas Neuropeptídeo Y/genética Neuropeptídeo Y/metabolismo Hormônio Liberador de Prolactina/administração & dosagem Distribuição Aleatória Ratos Ganho de Peso/efeitos dos fármacos [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Appetite Stimulants); 0 (Dietary Carbohydrates); 0 (Dietary Proteins); 0 (Neuropeptide Y); 0 (Prlh protein, rat); 0 (Prolactin-Releasing Hormone) [Em] Mês de entrada: 1601 [Cu] Atualização por classe: 151002 [Lr] Data última revisão: 151002 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150911 [St] Status: MEDLINE [do] DOI: 10.3945/jn.115.214338

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