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[PMID]:28461279
[Au] Autor:Yao A; Balchandani P; Shrivastava RK
[Ad] Endereço:Department of Neurosurgery, Icahn School of Medicine at Mount Sinai, Mount Sinai Health System, New York, New York, USA.
[Ti] Título:Metabolic In Vivo Visualization of Pituitary Adenomas: a Systematic Review of Imaging Modalities.
[So] Source:World Neurosurg;104:489-498, 2017 Aug.
[Is] ISSN:1878-8769
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Pituitary adenomas (PAs) are the most common intrasellar mass. Functional PAs constitute most of pituitary tumors and can produce symptoms related to hormonal overproduction. Timely and accurate detection is therefore of vital importance to prevent potentially irreversible sequelae. Magnetic resonance imaging is the gold standard for detecting PAs, but is limited by poor sensitivity for microadenomas and an inability to differentiate scar tissue from tumor residual or predict treatment response. Several new modalities that detect PAs have been proposed. METHODS: A systematic review of the PubMed database was performed for imaging studies of PAs since its inception. Data concerning study characteristics, clinical symptoms, imaging modalities, and diagnostic accuracy were collected. RESULTS: After applying exclusion criteria, 25 studies of imaging PAs using positron emission tomography (PET), magnetic resonance spectroscopy (MRS), and single photon emission computed tomography were reviewed. PET reliably detects PAs, particularly where magnetic resonance imaging is equivocal, although its efficacy is limited by high cost and low availability. Single photon emission computed tomography possesses good sensitivity for neuroendocrine tumors but its use with PAs is poorly documented. MRS consistently detects cellular proliferation and hormonal activity, but warrants further study at higher magnetic field strength. CONCLUSIONS: PET and MRS appear to have the strongest predictive value in detecting PAs. MRS has the advantage of low cost, but the literature is lacking in specific studies of the pituitary. Due to high recurrence rates of functional PAs and low sensitivity of existing diagnostic workups, further investigation of metabolic imaging is necessary.
[Mh] Termos MeSH primário: Adenoma/diagnóstico por imagem
Adenoma/metabolismo
Hormônios Ectópicos/metabolismo
Neuroimagem/métodos
Hormônios Hipofisários/metabolismo
Neoplasias Hipofisárias/diagnóstico por imagem
Neoplasias Hipofisárias/metabolismo
[Mh] Termos MeSH secundário: Adenoma/cirurgia
Seres Humanos
Imagem Tridimensional
Imagem por Ressonância Magnética
Espectroscopia de Ressonância Magnética
Neoplasias Hipofisárias/cirurgia
Tomografia por Emissão de Pósitrons
Sensibilidade e Especificidade
Tomografia Computadorizada de Emissão de Fóton Único
Tomografia Computadorizada por Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Hormones, Ectopic); 0 (Pituitary Hormones)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  2 / 4046 MEDLINE  
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[PMID]:28882981
[Au] Autor:Bonomi M; Vezzoli V; Krausz C; Guizzardi F; Vezzani S; Simoni M; Bassi I; Duminuco P; Di Iorgi N; Giavoli C; Pizzocaro A; Russo G; Moro M; Fatti L; Ferlin A; Mazzanti L; Zatelli MC; Cannavò S; Isidori AM; Pincelli AI; Prodam F; Mancini A; Limone P; Tanda ML; Gaudino R; Salerno M; Francesca P; Maghnie M; Maggi M; Persani L; Italian Network on Central Hypogonadism
[Ad] Endereço:Department of Clinical Sciences and Community HealthUniversity of Milan, Milan, Italy m.bonomi@auxologico.it marco.bonomi@unimi.it.
[Ti] Título:Characteristics of a nationwide cohort of patients presenting with isolated hypogonadotropic hypogonadism (IHH).
[So] Source:Eur J Endocrinol;178(1):23-32, 2018 Jan.
[Is] ISSN:1479-683X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Isolated hypogonadotropic hypogonadism (IHH) is a rare disorder with pubertal delay, normal (normoosmic-IHH, nIHH) or defective sense of smell (Kallmann syndrome, KS). Other reproductive and non-reproductive anomalies might be present although information on their frequency are scanty, particularly according to the age of presentation. DESIGN: Observational cohort study carried out between January 2008 and June 2016 within a national network of academic or general hospitals. METHODS: We performed a detailed phenotyping of 503 IHH patients with: (1) manifestations of hypogonadism with low sex steroid hormone and low/normal gonadotropins; (2) absence of expansive hypothalamic/pituitary lesions or multiple pituitary hormone defects. Cohort was divided on IHH onset (PPO, pre-pubertal onset or AO, adult onset) and olfactory function: PPO-nIHH ( = 275), KS ( = 184), AO-nIHH ( = 36) and AO-doIHH (AO-IHH with defective olfaction, = 8). RESULTS: 90% of patients were classified as PPO and 10% as AO. Typical midline and olfactory defects, bimanual synkinesis and familiarity for pubertal delay were also found among the AO-IHH. Mean age at diagnosis was significantly earlier and more frequently associated with congenital hypogonadism stigmata in patients with Kallmann's syndrome (KS). Synkinesis, renal and male genital tract anomalies were enriched in KS. Overweight/obesity are significantly associated with AO-IHH rather than PPO-IHH. CONCLUSIONS: Patients with KS are more prone to develop a severe and complex phenotype than nIHH. The presence of typical extra-gonadal defects and familiarity for PPO-IHH among the AO-IHH patients indicates a common predisposition with variable clinical expression. Overall, these findings improve the understanding of IHH and may have a positive impact on the management of patients and their families.
[Mh] Termos MeSH primário: Hipogonadismo/fisiopatologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idade de Início
Estudos de Coortes
Feminino
Hormônios Esteroides Gonadais/sangue
Gonadotropinas/sangue
Gonadotropinas/deficiência
Seres Humanos
Hipogonadismo/diagnóstico por imagem
Hipogonadismo/epidemiologia
Itália/epidemiologia
Masculino
Obesidade/complicações
Obesidade/epidemiologia
Transtornos do Olfato/complicações
Transtornos do Olfato/epidemiologia
Sobrepeso/complicações
Sobrepeso/epidemiologia
Fenótipo
Hormônios Hipofisários/sangue
Hormônios Hipofisários/deficiência
Sincinesia/complicações
Sincinesia/epidemiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Gonadal Steroid Hormones); 0 (Gonadotropins); 0 (Pituitary Hormones)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171129
[Lr] Data última revisão:
171129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1530/EJE-17-0065


  3 / 4046 MEDLINE  
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[PMID]:28579534
[Au] Autor:Rojo-Bartolomé I; Martínez-Miguel L; Lafont AG; Vílchez MC; Asturiano JF; Pérez L; Cancio I
[Ad] Endereço:CBET Research Group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology of Plentzia (PIE-UPV/EHU), University of the Basque Country (UPV/EHU), Areatza, z/g, E-48620 Plentzia, Basque Country, Spain.
[Ti] Título:Molecular markers of oocyte differentiation in European eel during hormonally induced oogenesis.
[So] Source:Comp Biochem Physiol A Mol Integr Physiol;211:17-25, 2017 Sep.
[Is] ISSN:1531-4332
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reproduction in captivity is a key study issue in Anguilla anguilla as a possible solution for its dwindling population. Understanding the mechanisms controlling the production of ribosomal building blocks during artificially induced oocyte maturation could be particularly interesting. Transcription levels of ribosomal biogenesis associated genes could be used as markers to monitor oogenesis. Eels from the Albufera Lagoon were injected with carp pituitary extract for 15weeks and ovaries in previtellogenic (PV) stage (non-injected), in early-, mid-, late-vitellogenesis (EV, MV, LV), as well as in migratory nucleus stage (MN) were analysed. 5S rRNA and related genes were highly transcribed in ovaries with PV oocytes. As oocytes developed, transcriptional levels of genes related to 5S rRNA production (gtf3a), accumulation (gtf3a, 42sp43) and nucleocytoplasmic transport (rpl5, rpl11) and the 5S/18S rRNA index decreased (PV>EV>MV>LV>MN). On the contrary, 18S rRNA was at its highest at MN stage while ubtf1 in charge of activating RNA-polymerase I and synthesising 18S rRNA behaved as 5S related genes. Individuals that did not respond (NR) to the treatment showed 5S/18S index values similar to PV females, while studied genes showed EV/LV-like transcription levels. Therefore, NR females fail to express the largest rRNAs, which could thus be taken as markers of successful vitellogenesis progression. In conclusion, we have proved that the transcriptional dynamics of ribosomal genes provides useful tools to characterize induced ovarian development in European eels. In the future, such markers should be studied as putative indicators of response to hormonal treatments and of the quality of obtained eel oocytes.
[Mh] Termos MeSH primário: Diferenciação Celular/genética
Enguias/genética
Oócitos/citologia
Oogênese/fisiologia
Hormônios Hipofisários/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Feminino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pituitary Hormones)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE


  4 / 4046 MEDLINE  
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[PMID]:28570646
[Au] Autor:McGregor R; Shan L; Wu MF; Siegel JM
[Ad] Endereço:Department of Psychiatry and Biobehavioral Sciences, University of California at Los Angeles, Los Angeles, California, United States of America.
[Ti] Título:Diurnal fluctuation in the number of hypocretin/orexin and histamine producing: Implication for understanding and treating neuronal loss.
[So] Source:PLoS One;12(6):e0178573, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The loss of specific neuronal phenotypes, as determined by immunohistochemistry, has become a powerful tool for identifying the nature and cause of neurological diseases. Here we show that the number of neurons identified and quantified using this method misses a substantial percentage of extant neurons in a phenotype specific manner. In mice, 24% more hypocretin/orexin (Hcrt) neurons are seen in the night compared to the day, and an additional 17% are seen after inhibiting microtubule polymerization with colchicine. We see no such difference between the number of MCH (melanin concentrating hormone) neurons in dark, light or colchicine conditions, despite MCH and Hcrt both being hypothalamic peptide transmitters. Although the size of Hcrt neurons did not differ between light and dark, the size of MCH neurons was increased by 15% in the light phase. The number of neurons containing histidine decarboxylase (HDC), the histamine synthesizing enzyme, was 34% greater in the dark than in the light, but, like Hcrt, cell size did not differ. We did not find a significant difference in the number or the size of neurons expressing choline acetyltransferase (ChAT), the acetylcholine synthesizing enzyme, in the horizontal diagonal band (HBD) during the dark and light conditions. As expected, colchicine treatment did not increase the number of these neurons. Understanding the function and dynamics of transmitter production within "non-visible" phenotypically defined cells has fundamental implications for our understanding of brain plasticity.
[Mh] Termos MeSH primário: Ritmo Circadiano
Histamina/biossíntese
Neurônios/metabolismo
Orexinas/biossíntese
[Mh] Termos MeSH secundário: Animais
Colina O-Acetiltransferase/metabolismo
Colchicina/administração & dosagem
Histidina Descarboxilase/metabolismo
Hormônios Hipotalâmicos/metabolismo
Masculino
Melaninas/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Neurônios/citologia
Neurônios/enzimologia
Hormônios Hipofisários/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypothalamic Hormones); 0 (Melanins); 0 (Orexins); 0 (Pituitary Hormones); 67382-96-1 (melanin-concentrating hormone); 820484N8I3 (Histamine); EC 2.3.1.6 (Choline O-Acetyltransferase); EC 4.1.1.22 (Histidine Decarboxylase); SML2Y3J35T (Colchicine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178573


  5 / 4046 MEDLINE  
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[PMID]:28511597
[Au] Autor:Equihua-Benítez AC; Guzmán-Vásquez K; Drucker-Colín R
[Ad] Endereço:a Departamento de Neuropatología Molecular , Instituto de Fisiología Celular, Universidad Nacional Autónoma de México , Ciudad de México , México.
[Ti] Título:Understanding sleep-wake mechanisms and drug discovery.
[So] Source:Expert Opin Drug Discov;12(7):643-657, 2017 Jul.
[Is] ISSN:1746-045X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Although not discernible at first glance, sleep is a highly active and regulated brain state. Although we spend practically one third of our lifetimes in this stage, its importance is often taken for granted. Sleep loss can lead to disease, error and economic loss. Our understanding of how sleep is achieved has greatly advanced in recent years, and with that, the management of sleep disorders has improved. There is still room for improvement and recently many new compounds have reached clinical trials with a few being approved for commercial use. Areas covered: In this review, the authors make the case of sleep disorders as a matter of public health. The mechanisms of sleep transition are discussed emphasizing the wake and sleep promoting interaction of different brain regions. Finally, advances in pharmacotherapy are examined in the context of chronic insomnia and narcolepsy. Expert opinion: The orexinergic system is an example of a breakthrough in sleep medicine that has catalyzed drug development. Nevertheless, sleep is a topic still with many unanswered questions. That being said, the melanin-concentrating hormone system is becoming increasingly relevant and we speculate it will be the next target of sleep medication.
[Mh] Termos MeSH primário: Descoberta de Drogas/métodos
Transtornos do Sono-Vigília/tratamento farmacológico
Sono/fisiologia
[Mh] Termos MeSH secundário: Animais
Desenho de Drogas
Seres Humanos
Hormônios Hipotalâmicos/metabolismo
Melaninas/metabolismo
Narcolepsia/tratamento farmacológico
Narcolepsia/fisiopatologia
Hormônios Hipofisários/metabolismo
Distúrbios do Início e da Manutenção do Sono/tratamento farmacológico
Distúrbios do Início e da Manutenção do Sono/fisiopatologia
Transtornos do Sono-Vigília/fisiopatologia
Vigília/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Hypothalamic Hormones); 0 (Melanins); 0 (Pituitary Hormones); 67382-96-1 (melanin-concentrating hormone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170518
[St] Status:MEDLINE
[do] DOI:10.1080/17460441.2017.1329818


  6 / 4046 MEDLINE  
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[PMID]:28423326
[Au] Autor:Nectow AR; Moya MV; Ekstrand MI; Mousa A; McGuire KL; Sferrazza CE; Field BC; Rabinowitz GS; Sawicka K; Liang Y; Friedman JM; Heintz N; Schmidt EF
[Ad] Endereço:Laboratory of Molecular Genetics, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA; Princeton Neuroscience Institute, Princeton University, Lot 20 Washington Road, Princeton, NJ 08544, USA. Electronic address: anectow@princeton.edu.
[Ti] Título:Rapid Molecular Profiling of Defined Cell Types Using Viral TRAP.
[So] Source:Cell Rep;19(3):655-667, 2017 Apr 18.
[Is] ISSN:2211-1247
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Translational profiling methodologies enable the systematic characterization of cell types in complex tissues, such as the mammalian brain, where neuronal isolation is exceptionally difficult. Here, we report a versatile strategy for profiling CNS cell types in a spatiotemporally restricted fashion by engineering a Cre-dependent adeno-associated virus expressing an EGFP-tagged ribosomal protein (AAV-FLEX-EGFPL10a) to access translating mRNAs by translating ribosome affinity purification (TRAP). We demonstrate the utility of this AAV to target a variety of genetically and anatomically defined neural populations expressing Cre recombinase and illustrate the ability of this viral TRAP (vTRAP) approach to recapitulate the molecular profiles obtained by bacTRAP in corticothalamic neurons across multiple serotypes. Furthermore, spatially restricting adeno-associated virus (AAV) injections enabled the elucidation of regional differences in gene expression within this cell type. Altogether, these results establish the broad applicability of the vTRAP strategy for the molecular dissection of any CNS or peripheral cell type that can be engineered to express Cre.
[Mh] Termos MeSH primário: Cromatografia de Afinidade/métodos
Biossíntese de Proteínas
Ribossomos/metabolismo
Vírus/metabolismo
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Dependovirus/metabolismo
Feminino
Regulação da Expressão Gênica
Proteínas de Fluorescência Verde/metabolismo
Hormônios Hipotalâmicos/metabolismo
Hipotálamo/metabolismo
Masculino
Melaninas/metabolismo
Camundongos
Neurônios/metabolismo
Hormônios Hipofisários/metabolismo
Reprodutibilidade dos Testes
Sorotipagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Hypothalamic Hormones); 0 (Melanins); 0 (Pituitary Hormones); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins); 67382-96-1 (melanin-concentrating hormone)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE


  7 / 4046 MEDLINE  
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[PMID]:28389909
[Au] Autor:Wiechno PJ; Kowalska M; Kucharz J; Sadowska M; Michalski W; Poniatowska G; Jonska-Gmyrek J; Rzymkowska J; Nietupski K; Demkow T
[Ad] Endereço:Department of Uro-Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Roentgena 5 st., 02-781, Warsaw, Poland.
[Ti] Título:Dynamics of hormonal disorders following unilateral orchiectomy for a testicular tumor.
[So] Source:Med Oncol;34(5):84, 2017 May.
[Is] ISSN:1559-131X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Testicular tumors and their treatment interfere with homeostasis, hormonal status included. The aim of the study was to evaluate hormonal disorders of the pituitary-gonadal axis in men treated for testicular tumors. One hundred twenty-eight men treated for a unilateral testicular tumor at our institution were included. The hormonal status was prospectively evaluated in 62 patients before orchiectomy, 120 patients 1 month after orchiectomy and 110 patients at least 1 year after the treatment. The concentrations of human chorionic gonadotropin (hCG), testosterone (T), estradiol, luteinizing hormone (LH), follicle-stimulating hormone (FSH) and prolactin were measured. The clinically significant testosterone deficiency was defined either as testosterone <2.31 ng/mL or testosterone within the range of 2.31-3.46 ng/mL but simultaneous with T/LH ratio ≤1. Changes in hormone levels were significant: LH and FSH rose in the course of observation, and the concentration of hCG, testosterone, estradiol decreased. PRL concentration was the lowest at 1 month after orchiectomy. In multivariate analysis, the risk of the clinically significant testosterone deficiency was 0.2107 (95% CI 0.1206-0.3419) prior to orchiectomy, 0.3894 (95% CI 0.2983-0.4889) 1 month after surgery and 0.4972 (95% CI 0.3951-0.5995) 1 year after the treatment. The estradiol concentration was elevated in 40% of patients with recently diagnosed testicular cancer and that was correlated with a higher risk of testosterone deficiency after the treatment completion. Hormonal disorders of the pituitary-gonadal axis in men treated for testicular tumors are frequent. The malignant tissue triggers paraneoplastic disorders that additionally disturb the hormonal equilibrium.
[Mh] Termos MeSH primário: Hormônios Gonadais/metabolismo
Orquiectomia/efeitos adversos
Hormônios Hipofisários/metabolismo
Neoplasias Testiculares/cirurgia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Gonadotropina Coriônica/metabolismo
Estradiol/metabolismo
Hormônio Foliculoestimulante/metabolismo
Seres Humanos
Hormônio Luteinizante/metabolismo
Masculino
Meia-Idade
Orquiectomia/métodos
Hipófise/metabolismo
Prolactina/metabolismo
Testículo/metabolismo
Testosterona/deficiência
Testosterona/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chorionic Gonadotropin); 0 (Gonadal Hormones); 0 (Pituitary Hormones); 3XMK78S47O (Testosterone); 4TI98Z838E (Estradiol); 9002-62-4 (Prolactin); 9002-67-9 (Luteinizing Hormone); 9002-68-0 (Follicle Stimulating Hormone)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170409
[St] Status:MEDLINE
[do] DOI:10.1007/s12032-017-0943-0


  8 / 4046 MEDLINE  
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[PMID]:28353251
[Au] Autor:Krishnamurthy D; Rahmoune H; Guest PC
[Ad] Endereço:Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
[Ti] Título:Proteomic Profiling of the Pituitary Gland in Studies of Psychiatric Disorders.
[So] Source:Adv Exp Med Biol;974:313-319, 2017.
[Is] ISSN:0065-2598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Psychiatric disorders have been associated with perturbations of the hypothalamic-pituitary-adrenal axis. Therefore, proteomic studies of the pituitary gland have the potential to provide new insights into the underlying pathways affected in these conditions as well as identify new biomarkers or targets for use in developing improved medications. This chapter describes a protocol for preparation of pituitary protein extracts followed by characterization of the pituitary proteome by label-free liquid chromatography-tandem mass spectrometry in expression mode (LC-MS ). The main focus was on establishing a method for identifying the major pituitary hormones and accessory proteins as many of these have already been implicated in psychiatric diseases.
[Mh] Termos MeSH primário: Transtornos Mentais/metabolismo
Proteínas do Tecido Nervoso/análise
Hipófise/química
Pró-Proteína Convertases/análise
Proteômica/métodos
Espectrometria de Massas por Ionização por Electrospray/métodos
[Mh] Termos MeSH secundário: Cromatografia Líquida/métodos
Seres Humanos
Proteínas do Tecido Nervoso/metabolismo
Hipófise/metabolismo
Hormônios Hipofisários/metabolismo
Pró-Proteína Convertases/metabolismo
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nerve Tissue Proteins); 0 (Pituitary Hormones); EC 3.4.21.- (Proprotein Convertases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.1007/978-3-319-52479-5_30


  9 / 4046 MEDLINE  
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[PMID]:28323939
[Au] Autor:Hu G; He M; Ko WKW; Wong AOL
[Ad] Endereço:College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
[Ti] Título:TAC1 Gene Products Regulate Pituitary Hormone Secretion and Gene Expression in Prepubertal Grass Carp Pituitary Cells.
[So] Source:Endocrinology;158(6):1776-1797, 2017 Jun 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tachykinin-1 (TAC1) is known to have diverse functions in mammals, but similar information is scarce in fish species. Using grass carp as a model, the pituitary actions, receptor specificity and postreceptor signaling of TAC1 gene products, namely substance P (SP) and neurokinin A (NKA), were examined. TAC1 encoding SP and NKA as well as tachykinin receptors NK1R and NK2R were cloned in the carp pituitary. The newly cloned receptors were shown to be functional with properties similar to mammalian counterparts. In carp pituitary cells, SP and NKA could trigger luteinizing hormone (LH), prolactin (PRL), and somatolactin α (SLα) secretion, with parallel rises in PRL and SLα transcripts. Short-term SP treatment (3 hours) induced LH release, whereas prolonged induction (24 hours) could attenuate LHß messenger RNA (mRNA) expression. At pituitary cell level, LH, PRL, and SLα regulation by TAC1 gene products were mediated by NK1R, NK2R, and NK3R, respectively. Apparently, SP- and NKA-induced LH and SLα secretion and transcript expression were mediated by adenylyl cyclase/cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA), phospholiphase C (PLC)/inositol 1,4,5-triphosphate/protein kinase C (PKC), and Ca2+/calmodulin (CaM)/CaM-dependent protein kinase-II pathways. The signal transduction for PRL responses was similar, except for the absence of a PKC component. Regarding SP inhibition of LHß mRNA expression, the cAMP/PKA- and PLC/PKC-dependent (but not Ca2+/CaM-dependent) cascades were involved. These results, as a whole, suggest that TAC1 gene products play a role in LH, PRL, and SLα regulation via overlapping postreceptor signaling coupled to different subtypes of tachykinin receptor expressed in the carp pituitary.
[Mh] Termos MeSH primário: Carpas
Hormônios Hipofisários/genética
Hormônios Hipofisários/secreção
Taquicininas/fisiologia
[Mh] Termos MeSH secundário: Animais
Carpas/genética
Carpas/metabolismo
Clonagem Molecular
Feminino
Proteínas de Peixes/genética
Proteínas de Peixes/fisiologia
Expressão Gênica
Células HEK293
Seres Humanos
Masculino
Hipófise/citologia
Hipófise/metabolismo
Maturidade Sexual/fisiologia
Transdução de Sinais/genética
Taquicininas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Pituitary Hormones); 0 (Tachykinins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1210/en.2016-1740


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[PMID]:28322083
[Au] Autor:Gerics B; Szalay F; Sótonyi P; Jancsik V
[Ad] Endereço:Department of Anatomy and Histology, University of Veterinary Medicine , H-1078 Budapest, István utca 2 , Hungary.
[Ti] Título:Diurnal variation of the melanin-concentrating hormone level in the hypothalamus.
[So] Source:Acta Biol Hung;68(1):14-21, 2017 Mar.
[Is] ISSN:0236-5383
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:Melanin-concentrating hormone (MCH), the neuropeptide produced mainly in the hypothalamus, plays an operative role in regulating food intake and the sleep/wake cycle. Considering that these physiological functions pursue diurnal variations, we checked whether the total hypothalamic MCH level depends on the time of the day. The aggregated MCH peptide content of the whole MCH neuron population was significantly higher at the end of the sleeping period (lights on), than at the end of the active period (lights off). This result, together with earlier observations, indicates that in contrast to the MCH gene expression, the level of MCH peptide is object of circadian variation in the hypothalamus.
[Mh] Termos MeSH primário: Ritmo Circadiano
Hormônios Hipotalâmicos/metabolismo
Hipotálamo/metabolismo
Melaninas/metabolismo
Hormônios Hipofisários/metabolismo
[Mh] Termos MeSH secundário: Animais
Hipotálamo/citologia
Imuno-Histoquímica
Masculino
Camundongos Endogâmicos
Neurônios/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypothalamic Hormones); 0 (Melanins); 0 (Pituitary Hormones); 67382-96-1 (melanin-concentrating hormone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1556/018.68.2017.1.2



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