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[PMID]:28501652
[Au] Autor:Zappa-Villar MF; López-León M; Pardo J; Costa M; Crespo R; Dardenne M; Goya RG; Reggiani PC
[Ad] Endereço:Institute for Biochemical Research-Histology B-Pathology B, Faculty of Medicine, National University of La Plata, Argentina.
[Ti] Título:A new adenovector system for implementing thymulin gene therapy for inflammatory disorders.
[So] Source:Mol Immunol;87:180-187, 2017 Jul.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Thymulin is a thymic peptide possessing anti-inflammatory effects. In order to manipulate thymulin expression in gene therapy studies, we built a bidirectional regulatable two-vector Tet-Off system and the corresponding control system. The experimental two-vector system, ETV, consists of a recombinant adenovector (RAd) harboring an expression cassette centered on a Tet-Off bidirectional promoter flanked by a synthetic gene for thymulin and the gene for humanized Green Fluorescent Protein (hGFP). The second adenovector of this system, RAd-tTA, constitutively expresses the regulatory protein tTA. When cells are co-transduced by the two adenovector components, tTA activates the bidirectional promoter and both transgenes are expressed. In the presence of the antibiotic doxycycline (DOX) transgene expression is deactivated. The control two-vector system, termed CTV, is similar to ETV but only expresses hGFP. In CHO-K1, BHK, and C2C12 cells, ETV and CTV induced a dose-dependent hGFP expression. In CHO-K1 cells, transgene expression was almost completely inhibited by DOX (1mg/ml). After intracerebroventricular injection of ETV in rats, thymulin levels increased significantly in the cerebrospinal fluid and there was high hGFP expression in the ependymal cell layer. When injected intramuscularly the ETV system induced a progressive increase in serum thymulin levels, which were inhibited when DOX was added to the drinking water. We conclude that our regulatable two-adenovector system is an effective molecular tool for implementing short and long-term anti-inflammatory thymulin gene therapy in animal models of acute or chronic inflammation.
[Mh] Termos MeSH primário: Adenoviridae/genética
Vetores Genéticos/genética
Inflamação/genética
Inflamação/terapia
Fator Tímico Circulante/genética
[Mh] Termos MeSH secundário: Adenoviridae/efeitos dos fármacos
Animais
Células CHO
Linhagem Celular
Cricetulus
Doxiciclina/farmacologia
Feminino
Terapia Genética/métodos
Proteínas de Fluorescência Verde/genética
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Modelos Animais
Ratos
Ratos Sprague-Dawley
Transgenes/efeitos dos fármacos
Transgenes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
147336-22-9 (Green Fluorescent Proteins); 9H198D04WL (Thymic Factor, Circulating); N12000U13O (Doxycycline)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170515
[St] Status:MEDLINE


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[PMID]:28281875
[Au] Autor:Lunin SM; Khrenov MO; Glushkova OV; Vinogradova EV; Yashin VA; Fesenko EE; Novoselova EG
[Ad] Endereço:Institute of Cell Biophysics, Pushchino, Moscow region, Russia.
[Ti] Título:Extrathymic production of thymulin induced by oxidative stress, heat shock, apoptosis, or necrosis.
[So] Source:Int J Immunopathol Pharmacol;30(1):58-69, 2017 Mar.
[Is] ISSN:2058-7384
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Thymic peptides are immune regulators produced mainly in the thymus. However, thymic peptides such as thymosin-α and thymopoietin have precursors widely expressed outside the thymus, localized in cell nuclei, and involved in vital nuclear functions. In stress-related conditions, they can relocalize. We hypothesized that another thymic peptide, thymulin, could be similarly produced by non-thymic cells during stress and have a precursor therein. Non-thymic cells, including macrophages and fibroblasts, were exposed to oxidative stress, heat, apoptosis, or necrosis. Extracellular thymulin was identified in media of both cell types 2 h after exposure to stress or lethal signals. Therefore, thymulin is released by non-thymic cells. To examine possible thymulin precursors in non-thymic cells, macrophage lysates were analyzed by western blotting. Bands stained with anti-thymulin antibody were detected in two locations, approximately 60 kDa and 10 kDa, which may be a possible precursor and intermediate. All of the exposures except for heat were effective for induction of the 10 kDa protein. BLAST search using thymulin sequence identified SPATS2L, an intranucleolar stress-response protein with molecular weight of 62 kDa, containing thymulin-like sequence. Comparisons of blots stained with anti-thymulin and anti-SPATS2L antibodies indicate that SPATS2L may be a possible candidate for the precursor of thymulin.
[Mh] Termos MeSH primário: Fibroblastos/metabolismo
Macrófagos/metabolismo
Fator Tímico Circulante/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose
Caspase 3/metabolismo
Linhagem Celular
Proteínas de Choque Térmico HSP72/metabolismo
Temperatura Alta
Camundongos
Necrose
Estresse Oxidativo
Células RAW 264.7
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HSP72 Heat-Shock Proteins); 9H198D04WL (Thymic Factor, Circulating); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1177/0394632017694625


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[PMID]:27473547
[Au] Autor:Siqueira CM; Motta PD; Cardoso TN; de Paula Coelho C; Popi AF; Couceiro JN; Bonamin LV; Holandino C
[Ad] Endereço:Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Federal Institute of Rio de Janeiro, Brazil.
[Ti] Título:Homeopathic treatments modify inflammation but not behavioral response to influenza antigen challenge in BALB/c mice.
[So] Source:Homeopathy;105(3):257-264, 2016 Aug.
[Is] ISSN:1476-4245
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Influenza affects thousands of people worldwide every year, motivating the development of new therapies. In this work, the effects of two homeopathic preparations (influenza biotherapies and thymulin) were chosen following two different rationales: isotherapy and endo-isotherapy models. The homeopathic effects were evaluated individually considering the inflammatory and behavioral responses against influenza virus antigen were studied in BALB/c mice. METHODS: Male adult mice were treated orally and blindly for 21 days with highly diluted influenza virus or with thymulin, and were divided in two sets of experiments. The first series of experiments aimed to describe their behavior, using an open field (OF) device. In the second series, mice were challenged subcutaneously with influenza hemagglutinin antigen (7 µg/200 µl) at day 21. At day 42, behavior and inflammation response were evaluated. RESULTS: No behavioral changes were seen in OF tests at any time point after treatments. Flow cytometry and morphometry revealed significant changes in T and B cell balance after influenza antigen challenge, varying according to treatment. CONCLUSION: The results show that both homeopathic treatments induced subtle changes in acquired immune anti-viral response regulation. A deeper understanding of the mechanism could elucidate their possible use in influenza epidemiological situations.
[Mh] Termos MeSH primário: Comportamento Animal
Inflamação/terapia
Vírus da Influenza A Subtipo H3N2
Infecções por Orthomyxoviridae/terapia
Fator Tímico Circulante/química
[Mh] Termos MeSH secundário: Animais
Linfócitos B/imunologia
Glicoproteínas de Hemaglutininação de Vírus da Influenza/imunologia
Homeopatia
Masculino
Camundongos Endogâmicos BALB C
Infecções por Orthomyxoviridae/imunologia
Distribuição Aleatória
Subpopulações de Linfócitos T/imunologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hemagglutinin Glycoproteins, Influenza Virus); 9H198D04WL (Thymic Factor, Circulating)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160731
[St] Status:MEDLINE


  4 / 596 MEDLINE  
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[PMID]:26690976
[Au] Autor:Novoselova EG; Glushkova OV; Lunin SM; Khrenov MO; Novoselova TV; Parfenyuk SB; Fesenko EE
[Ad] Endereço:Institute of Cell Biophysics, Pushchino, Moscow region, Russia.
[Ti] Título:Signaling, stress response and apoptosis in pre-diabetes and diabetes: restoring immune balance in mice with alloxan-induced type 1 diabetes mellitus.
[So] Source:Int Immunopharmacol;31:24-31, 2016 Feb.
[Is] ISSN:1878-1705
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to compare immune imbalances in "pre-diabetic" and diabetic mice and to evaluate the efficacy of several agents in improving the immunity of mice with type 1 diabetes. Pre-diabetic and diabetic models generated by a single or double alloxan injection were monitored for plasma glucose and pancreas immunohistochemistry. To study the immunity in pre-diabetic and diabetic Balb/C male mice; the levels of cytokines; synthesis of inducible heat shock proteins HSP72 and HSP90α; activity of the NF-κB, IFR3, SAPK/JNK, and TLR4 pathways; and apoptosis levels in thymuses were measured. Pre-diabetes resulted in a decrease in IL-4, IL-5 and IL-10 in plasma; in diabetic mice, plasma IFN-gamma, IL-6, TNF-alpha, and IL-10 were decreased. The NF-κB alternative pathway activity and TLR4 expression were significantly increased only in pre-diabetic mice, whereas SAPK/JNK activation was observed at both stages of diabetes. Other measured parameters also showed distinct altered patterns in the immunity of pre-diabetic and diabetic mice. Treatment with an inhibitor of NF-κB, thymulin, or a diet with an antioxidant improved or normalized the immune balance in diabetic mice and also notably decreased pancreatic cell damage in pre-diabetic mice.
[Mh] Termos MeSH primário: Antioxidantes/administração & dosagem
Diabetes Mellitus Tipo 1/tratamento farmacológico
Células Secretoras de Insulina/efeitos dos fármacos
Fator Tímico Circulante/administração & dosagem
[Mh] Termos MeSH secundário: Aloxano/administração & dosagem
Animais
Antioxidantes/farmacologia
Apoptose/efeitos dos fármacos
Citocinas/metabolismo
Diabetes Mellitus Tipo 1/induzido quimicamente
Diabetes Mellitus Tipo 1/imunologia
Seres Humanos
Células Secretoras de Insulina/fisiologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Proteína Quinase 8 Ativada por Mitógeno/metabolismo
NF-kappa B/antagonistas & inibidores
Transdução de Sinais/efeitos dos fármacos
Estresse Fisiológico/efeitos dos fármacos
Fator Tímico Circulante/farmacologia
Receptor 4 Toll-Like/genética
Receptor 4 Toll-Like/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Cytokines); 0 (NF-kappa B); 0 (Toll-Like Receptor 4); 6SW5YHA5NG (Alloxan); 9H198D04WL (Thymic Factor, Circulating); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 8)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151223
[St] Status:MEDLINE


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[PMID]:26845842
[Au] Autor:Labunets IF; Talanov SA; Vasilyev RG; Rodnichenko AE; Utko NA; Kyzminova IA; Kopjak BS; Podjachenko EV; Sagach VF; Butenko GM
[Ti] Título:[THYMIC HORMONES, ANTIOXIDANT ENZYMES AND NEUROGENESIS OF BULBUS OLFACTORIUS IN RATS WITH PARKINSONISM: THE EFFECT OF MELATONIN].
[So] Source:Fiziol Zh;61(5):35-45, 2015.
[Is] ISSN:2522-9028
[Cp] País de publicação:Ukraine
[La] Idioma:ukr
[Ab] Resumo:The adult rats received both neurotoxin 6-hidroxidophamine and neurotoxin and melatonin. It was investigated a link between the disturbances of the brain antioxidant enzymes activity and thymic endocrine function, as possible pathogenic factors of parkinsonism, with changes in the number of neural stem cells (NSC) in the bulbus olfactorius. Rats with motor asymmetry in the apomorphine test and significant damage of the dopaminergic neurons in the-substantia nigra have decreased levels of superoxide dismutase, catalase and glutathione peroxidase activities in striatum (1.3-1.4 times) and blood thymulin content (8 times) compared to control group. On the contrary, examined indices were not changed in rats without motor asymmetry and correspondingly partly damaged neurons. The number of nestin(+)-cells in the bulbus olfactorius of rats without motor asymmetry increased from 91.2% to 99.3% and remained unchanged after melatonin administration course (10 mg/kg during 18 days). Melatonin administration resulted in the decrease in the number of nestin(+)-cells along with significant elevation of the decreased antioxidant enzymes activity and blood thymulin content in rats with circulatory movements. Possibilities of the enhancement of NSC differentiation in bulbus olfactorius into neuronal direction in such animals has been discussed. The conclusion about the potential use of melatonin as a neuroprotector in parkinsonism therapy has been made.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Ataxia/prevenção & controle
Melatonina/farmacologia
Fármacos Neuroprotetores/farmacologia
Bulbo Olfatório/efeitos dos fármacos
Doença de Parkinson Secundária/tratamento farmacológico
Timo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Apomorfina/antagonistas & inibidores
Apomorfina/farmacologia
Ataxia/induzido quimicamente
Ataxia/genética
Ataxia/patologia
Catalase/genética
Catalase/metabolismo
Contagem de Células
Diferenciação Celular/efeitos dos fármacos
Agonistas de Dopamina/farmacologia
Expressão Gênica
Glutationa Peroxidase/genética
Glutationa Peroxidase/metabolismo
Masculino
Nestina/genética
Nestina/metabolismo
Células-Tronco Neurais/citologia
Células-Tronco Neurais/efeitos dos fármacos
Células-Tronco Neurais/metabolismo
Bulbo Olfatório/metabolismo
Bulbo Olfatório/patologia
Oxidopamina
Doença de Parkinson Secundária/induzido quimicamente
Doença de Parkinson Secundária/genética
Doença de Parkinson Secundária/patologia
Ratos
Ratos Wistar
Superóxido Dismutase/genética
Superóxido Dismutase/metabolismo
Fator Tímico Circulante/genética
Fator Tímico Circulante/metabolismo
Timo/metabolismo
Timo/patologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Dopamine Agonists); 0 (Nes protein, rat); 0 (Nestin); 0 (Neuroprotective Agents); 8HW4YBZ748 (Oxidopamine); 9H198D04WL (Thymic Factor, Circulating); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); JL5DK93RCL (Melatonin); N21FAR7B4S (Apomorphine)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:160205
[Lr] Data última revisão:
160205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160206
[St] Status:MEDLINE


  6 / 596 MEDLINE  
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[PMID]:26027280
[Au] Autor:Novoseletskaya AV; Kiseleva NM; Belova OV; Zimina IV; Inozemtsev AN; Arion VY; Sergienko VI
[Ti] Título:[The influence of the thymus peptides on analgesia caused by acute and chronic immobilization].
[So] Source:Vestn Ross Akad Med Nauk;(1):113-7, 2015.
[Is] ISSN:0869-6047
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:OBJECTIVE: Our aim was to investigate the influence of thymic polypeptides on pain sensitivity and to analyze a possible role of the opioid system in the implementation of the analgesia caused by immobilization stress. METHODS: The study was performed on male Wistar rats at the Moscow state University named after M. V. Lomonosov. We studied effects of thymus peptides: thymuline (0.15 mg/kg), fraction 5 thymosin (0.25 microgram/kg) and cattle thymus extracted product (CTEP) (0.5 mg/kg) on pain sensitivity in rats using test "tail flick" without stress, with acute (3 h) and sub acute (12 h) immobilization stress. The comparison groups were animals treated with saline and spleen polypeptides. RESULTS: It is shown that preparations of thymus increase the threshold of pain sensitivity in the intact animals. Immobilization stress duration 3 and 12 h in thymus peptides treated rats caused a less pronounced increase in pain threshold than in the control groups (immobilization stress 3 h: CTEP--p = 0.025, thymuline--p = 0.022, fraction 5 thymosin--p = 0.033; immobilization stress 12 h: CTEP--p = 0.034, thymuline--p = 0.027, fraction 5 thymosin--p = 0.036). The opioid receptor blocker naloxone (1 mg/kg) did not completely block the stress-induced analgesia, indicating the presence of both opioid and non -opioid components in this state. In thymus peptides treated rats, opioid component was less pronounced than in the control groups (CTEP--p = 0.031, thymuline--p = 0.026, fraction 5 thymosin--p = 0.029). CONCLUSION: Pre-activation of the opioid system by the thymus polypeptides leads to an increase in the share of non-opioid component of the stress-induced analgesia and prevents the depletion of the opioid system in immobilization stress.
[Mh] Termos MeSH primário: Naloxona/farmacologia
Dor
Restrição Física/efeitos adversos
Fator Tímico Circulante
Timosina
Timo/metabolismo
[Mh] Termos MeSH secundário: Analgesia/métodos
Animais
Bovinos
Masculino
Modelos Animais
Antagonistas de Entorpecentes/farmacologia
Dor/diagnóstico
Dor/tratamento farmacológico
Dor/etiologia
Dor/metabolismo
Dor/fisiopatologia
Manejo da Dor
Ratos
Ratos Wistar
Receptores Opioides/metabolismo
Fator Tímico Circulante/metabolismo
Fator Tímico Circulante/farmacologia
Timosina/metabolismo
Timosina/farmacologia
Extratos do Timo/metabolismo
Extratos do Timo/farmacologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Narcotic Antagonists); 0 (Receptors, Opioid); 0 (Thymus Extracts); 36B82AMQ7N (Naloxone); 61512-21-8 (Thymosin); 9H198D04WL (Thymic Factor, Circulating)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:161020
[Lr] Data última revisão:
161020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150602
[St] Status:MEDLINE


  7 / 596 MEDLINE  
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[PMID]:26016747
[Au] Autor:Su S; Sun X; Zhou X; Fang F; Li Y
[Ad] Endereço:College of Animal Sciences and TechnologyAnhui Agricultural University, 130, Changjiang West Road, Hefei, Anhui 230036, People's Republic of ChinaCollege of Life ScienceFujian Agriculture and Forestry University, Fuzhou 350002, People's Republic of ChinaThe Biotechnology Center of Anhui Agriculture
[Ti] Título:Effects of GnRH immunization on the reproductive axis and thymulin.
[So] Source:J Endocrinol;226(2):93-102, 2015 Aug.
[Is] ISSN:1479-6805
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The bidirectional regulation of thymulin in the reproductive-endocrine function of the hypothalamic-pituitary-gonadal (HPG) axis of rats immunized against GnRH remains largely unclear. We explored the alterations in hormones in the HPG axis in immunized rats to dissect the repressive effect of immunization on thymulin, and to clarify the interrelation of reproductive hormones and thymulin in vivo. The results showed that, in the first 2 weeks of booster immunization, thymulin was repressed when reproductive hormones were severely reduced. The self-feedback regulation of thymulin was then stimulated in later immune stages: the rising circulating thymulin upregulated LH and FSH, including GnRH in the hypothalamus, although the levels of those hormones were still significantly lower than in the control groups. In astrocytes, thymulin produced a feedback effect in regulated GnRH neurons. However, in the arcuate nucleus (Arc) and the median eminence (ME), the mediator of astrocytes and other glial cells were also directly affected by reproductive hormones. Thus, in immunized rats, the expression of glial fibrillary acidic protein was distinctly stimulated in the Arc and ME. This study demonstrated that thymulin was downregulated by immunization against GnRH in early stage. Subsequently, the self-feedback regulation was provoked by low circulating thymulin. Thereafter, rising thymulin levels promoted pituitary gonadotropins levels, while acting directly on GnRH neurons, which was mediated by astrocytes in a region-dependent manner in the hypothalamus.
[Mh] Termos MeSH primário: Hormônio Liberador de Gonadotropina/imunologia
Hipotálamo/metabolismo
Reprodução/fisiologia
Fator Tímico Circulante/metabolismo
[Mh] Termos MeSH secundário: Animais
Astrócitos/metabolismo
Hormônio Foliculoestimulante/sangue
Proteína Glial Fibrilar Ácida/metabolismo
Imunização
Hormônio Luteinizante/sangue
Masculino
Ratos
Ratos Sprague-Dawley
Testosterona/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glial Fibrillary Acidic Protein); 33515-09-2 (Gonadotropin-Releasing Hormone); 3XMK78S47O (Testosterone); 9002-67-9 (Luteinizing Hormone); 9002-68-0 (Follicle Stimulating Hormone); 9H198D04WL (Thymic Factor, Circulating)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150704
[Lr] Data última revisão:
150704
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150529
[St] Status:MEDLINE
[do] DOI:10.1530/JOE-14-0720


  8 / 596 MEDLINE  
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[PMID]:25662754
[Au] Autor:Lunin SM; Khrenov MO; Novoselova TV; Parfenyuk SB; Glushkova OV; Fesenko EE; Novoselova EG
[Ad] Endereço:Institute of Cell Biophysics, Russian Academy of Sciences, Russia. Electronic address: lunin@pochta.ru.
[Ti] Título:Modulation of inflammatory response in mice with severe autoimmune disease by thymic peptide thymulin and an inhibitor of NF-kappaB signalling.
[So] Source:Int Immunopharmacol;25(2):260-6, 2015 Apr.
[Is] ISSN:1878-1705
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To investigate some cellular and molecular aspects of the autoimmune response and anti-inflammatory efficiency of potential therapeutic agents in a severe form of experimental autoimmune encephalomyelitis (sEAE), an inhibitor of NF-kappaB signalling, IKK Inhibitor XII, and/or thymic peptide thymulin, were injected intraperitoneally at 1.8 and 0.15mg/kg e.o.d, respectively, to C57BL/6 mice immunized with myelin oligodendrocyte glycoprotein and several adjuvants. The immunization induced high lethality in three weeks. The biphasic cytokine response observed in earlier and delayed phases was attributed to the activity of Th1 and Th17 cells, respectively. Phosphorylation of RelA protein from the NF-kappaB family increased during the earlier phase and decreased in the delayed phase. SAPK/JNK signalling protein and heat shock protein Hsp72 significantly increased in lymphocytes. Both the IKK Inhibitor XII and thymulin reduced disease severity, attenuated immune imbalance, and increased mouse life-span. Co-administration of the agents produced no additive effect. Both the inhibitor and thymulin reduced the Th1 response but not the Th17 response. Therefore, RelA-associated Th1 activation and RelA-independent Th17 activation occurred in sEAE. Thymulin and the inhibitor demonstrate similar patterns of activity, potentially through the RelA pathway inhibition, resulting in a partial therapeutic effect on the animals' health status.
[Mh] Termos MeSH primário: Encefalomielite Autoimune Experimental/tratamento farmacológico
NF-kappa B/antagonistas & inibidores
Fator Tímico Circulante/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Citocinas/sangue
Encefalomielite Autoimune Experimental/sangue
Encefalomielite Autoimune Experimental/imunologia
Proteínas de Choque Térmico HSP72/imunologia
Linfócitos/efeitos dos fármacos
Linfócitos/imunologia
Masculino
Camundongos Endogâmicos C57BL
Proteínas Quinases Ativadas por Mitógeno/imunologia
NF-kappa B/imunologia
Transdução de Sinais/efeitos dos fármacos
Baço/citologia
Células Th1/imunologia
Células Th17/imunologia
Fator Tímico Circulante/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (HSP72 Heat-Shock Proteins); 0 (NF-kappa B); 9H198D04WL (Thymic Factor, Circulating); EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150324
[Lr] Data última revisão:
150324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150210
[St] Status:MEDLINE


  9 / 596 MEDLINE  
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[PMID]:25563001
[Au] Autor:Novoseletskaia AV; Kiseleva NM; Belova OV; Zimina IV; Moskvina SN; Bystrova OV; Inozemtsev AN; Arion VIa; Sergienko VI
[Ti] Título:[The impact of immunoactive drugs on passive avoidance response].
[So] Source:Vestn Ross Akad Med Nauk;(7-8):25-9, 2014.
[Is] ISSN:0869-6047
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:AIM: The objective of this project was to explore the influence of immunoactive drugs (tactivin, thymulin, and thymosin fraction 5) on the development of the passive avoidance conditioned reflex. MATERIALS AND METHODS: Two types of passive avoidance boxes were used--a regular two-chamber box and a modified three-chamber box, comprising a dark chamber in which rats were exposed to electrical shock, a safe dark chamber, and a light chamber in the center. RESULTS: The project has established that the memory trace persists longer under the influence of the immunoactive drugs in both models, which is consistent with the reference nootropic piracetam test results. Notably, the immunoactive drugs' mnemotropic effect was more pronounced in the modified three-chamber box than in the standard two-chamber box. Using the modified box helped to establish the influence of tactivin, thymulin, and thymosin fraction 5 on the spatial memory component. Immunotropic preparations from thymus caused the animals to select the safe chamber 24 hours later and in subsequent tests. CONCLUSION: The project's results indicate that the drugs tested do possess mnemotropic properties, so their range of clinical use can be broadened.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/farmacologia
Aprendizagem da Esquiva
Condicionamento Clássico
Memória
[Mh] Termos MeSH secundário: Animais
Aprendizagem da Esquiva/efeitos dos fármacos
Aprendizagem da Esquiva/fisiologia
Condicionamento Clássico/efeitos dos fármacos
Condicionamento Clássico/fisiologia
Reação de Fuga/efeitos dos fármacos
Reação de Fuga/fisiologia
Masculino
Memória/efeitos dos fármacos
Memória/fisiologia
Modelos Biológicos
Peptídeos/farmacologia
Ratos
Ratos Wistar
Fator Tímico Circulante/farmacologia
Timosina/análogos & derivados
Timosina/farmacologia
Extratos do Timo/farmacologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Peptides); 0 (Thymus Extracts); 0 (thymosin fraction 5); 61512-21-8 (Thymosin); 89492-35-3 (T-activin); 9H198D04WL (Thymic Factor, Circulating)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:161020
[Lr] Data última revisão:
161020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150108
[St] Status:MEDLINE


  10 / 596 MEDLINE  
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Laurenti, Márcia Dalastra
Texto completo
[PMID]:25439044
[Au] Autor:Rodrigues de Santana F; Coelho Cde P; Cardoso TN; Laurenti MD; Perez Hurtado EC; Bonamin LV
[Ad] Endereço:Research Center of Universidade Paulista, São Paulo, Brazil; Laboratory of Veterinary Pathology, Universidade de Santo Amaro, Brazil.
[Ti] Título:Modulation of inflammation response to murine cutaneous Leishmaniosis by homeopathic medicines: thymulin 5cH.
[So] Source:Homeopathy;103(4):275-84, 2014 Oct.
[Is] ISSN:1476-4245
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In previous studies, we observed that thymulin 5cH could modulate BCG (Bacillus Calmette-Guerin) induced chronic inflammation by increasing peritoneal B1 stem cells differentiation into phagocytes and improving phagocytosis efficiency. METHODS: We used the same protocol to study the effects of thymulin 5cH in the experimental murine Leishmaniasis, in order to elucidate some aspects of the parasite-host relation under this homeopathic treatment. Male Balb/c mice were orally treated with thymulin 5cH or vehicle during 60 days, after the subcutaneous inoculation of 2 × 10(6) units of Leishmania (L.) amazonensis into the footpad. Washied inflammatory cell suspension from peritoneal cavity, spleen, local lymph node and infected subcutaneous tissue were harvested after 2 and 60 days from infection to quantify the inflammation cells by flow cytometry and histometry methods. RESULTS: After a transitory increase of peritoneal T reg cells, treated mice presented, chronically, increase in the peritoneal and spleen B1 cells percentage (p = 0.0001) in relation to other cell types; more organized and exuberant inflammation response in the infection site, and decrease in the number of parasites per field inside the primary lesion (p = 0.05). No difference was seen in local lymph node histology. CONCLUSIONS: Thymulin 5cH is able to improve B1 cell activation and Leishmania (L) amazonensis phagocytosis efficiency in mice, similarly to that observed previously in BCG experimental infection.
[Mh] Termos MeSH primário: Homeopatia/métodos
Inflamação/tratamento farmacológico
Leishmaniose Cutânea/tratamento farmacológico
Leishmaniose Cutânea/imunologia
Fator Tímico Circulante/administração & dosagem
Fator Tímico Circulante/imunologia
[Mh] Termos MeSH secundário: Administração Oral
Animais
Modelos Animais de Doenças
Interações Hospedeiro-Parasita/efeitos dos fármacos
Inflamação/parasitologia
Linfonodos/efeitos dos fármacos
Linfonodos/parasitologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Baço/efeitos dos fármacos
Baço/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
9H198D04WL (Thymic Factor, Circulating)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:141202
[Lr] Data última revisão:
141202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE



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