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[PMID]:29186449
[Au] Autor:van der Spek AH; Jim KK; Karaczyn A; van Beeren HC; Ackermans MT; Darras VM; Vandenbroucke-Grauls CMJE; Hernandez A; Brouwer MC; Fliers E; van de Beek D; Boelen A
[Ad] Endereço:Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
[Ti] Título:The Thyroid Hormone Inactivating Type 3 Deiodinase Is Essential for Optimal Neutrophil Function: Observations From Three Species.
[So] Source:Endocrinology;159(2):826-835, 2018 02 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Neutrophils are essential effector cells of the innate immune system that have recently been recognized as thyroid hormone (TH) target cells. Cellular TH bioavailability is regulated by the deiodinase enzymes, which can activate or inactivate TH. We have previously shown that the TH inactivating enzyme type 3 deiodinase (D3) is present in neutrophils. Furthermore, D3 knockout (D3KO) mice show impaired bacterial killing upon infection. We hypothesized that D3 plays a role in neutrophil function during infection by actively regulating local TH availability. We measured TH concentrations in cerebrospinal fluid (CSF) from patients with bacterial meningitis and controls. Bacterial meningitis resulted in marked changes in CSF TH levels, characterized by a strong increase of thyroxine and reverse-triiodothyronine concentrations. This altered TH profile was consistent with elevated D3 activity in infiltrating neutrophils at the site of infection. D3 knockdown in zebrafish embryos with pneumococcal meningitis resulted in increased mortality and reduced neutrophil infiltration during infection. Finally, stimulated neutrophils from female D3KO mice exhibited impaired NADPH-oxidase activity, an important component of the neutrophil bacterial killing machinery. These consistent findings across experimental models strongly support a critical role for reduced intracellular TH concentrations in neutrophil function during infection, for which the TH inactivating enzyme D3 appears essential.
[Mh] Termos MeSH primário: Iodeto Peroxidase/fisiologia
Neutrófilos/fisiologia
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Estudos de Casos e Controles
Células Cultivadas
Embrião não Mamífero
Iodeto Peroxidase/genética
Meningites Bacterianas/líquido cefalorraquidiano
Meningites Bacterianas/imunologia
Camundongos Knockout
Especificidade da Espécie
Hormônios Tireóideos/líquido cefalorraquidiano
Hormônios Tireóideos/metabolismo
Tri-Iodotironina Reversa/líquido cefalorraquidiano
Peixe-Zebra/embriologia
Peixe-Zebra/genética
Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Thyroid Hormones); 5817-39-0 (Triiodothyronine, Reverse); EC 1.11.1.- (iodothyronine deiodinase type III); EC 1.11.1.8 (Iodide Peroxidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1210/en.2017-00666


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[PMID]:27857052
[Au] Autor:Jakowczuk M; Zalas D; Owecki M
[Ad] Endereço:Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland.
[Ti] Título:Permanent atrial fibrillation in heart failure patients as another condition with increased reverse triiodothyronine concentration.
[So] Source:Neuro Endocrinol Lett;37(4):337-342, 2016 Sep.
[Is] ISSN:0172-780X
[Cp] País de publicação:Sweden
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To fully investigate the thyroid hormonal function in patients with the most common arrhythmia - atrial fibrillation. MATERIALS AND METHODS: 120 patients (aged 55-85 yrs) with symptoms of congestive heart failure exacerbation and no other concomitant disorders (inclusion criteria: normal cardiac troponin T at admission and 12 hours after, normal renal, hepatic and respiratory function; exclusion criteria: inflammatory state, history of myocardial infarction). Depending on the presence of permanent atrial fibrillation (PAF), patients were divided into two groups: PAF (34 females, 26 males) and regular sinus heart rhythm (43 females, 17 males), the groups did not differ in terms of heart rate, blood pressure, presence of overt/subclinical thyroid dysfunction, and medical therapy used. In all subjects thyroid stimulating hormone, free thyroxine, free triiodothyronine, reverse triiodothyronine were measured; echocardiography was performed. RESULTS: PAF group showed higher FT4 and rT3 (1.41 vs. 1.27 ng/dl, p=0.0007; 0.61 vs. 0.32 ng/ml, p<0.0001, respectively). With ROC curve analysis the biochemical thyroid related factor of the highest prognostic value for PAF occurrence (with the highest sensitivity and specificity: 77% and 72%, respectively) was rT3 with the cut-off of above 0.3 ng/ml. Also, a positive correlation between rT3 levels and left ventricular posterior wall diameter was observed (Spearman's correlation coefficient 0.33, p=0.0093). CONCLUSIONS: PAF is another condition where an increase in rT3 is observed. rT3 concentration above 0.3 ng/ml may be a novel biochemical sign associated with the presence of PAF in patients with chronic heart failure.
[Mh] Termos MeSH primário: Fibrilação Atrial/sangue
Insuficiência Cardíaca/sangue
Hipertireoidismo/sangue
Hipotireoidismo/sangue
Tri-Iodotironina Reversa/sangue
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Fibrilação Atrial/complicações
Estudos de Casos e Controles
Estudos de Coortes
Feminino
Insuficiência Cardíaca/complicações
Seres Humanos
Hipertireoidismo/complicações
Hipotireoidismo/complicações
Masculino
Meia-Idade
Curva ROC
Testes de Função Tireóidea
Tireotropina/sangue
Tiroxina/sangue
Tri-Iodotironina/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
06LU7C9H1V (Triiodothyronine); 5817-39-0 (Triiodothyronine, Reverse); 9002-71-5 (Thyrotropin); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170221
[Lr] Data última revisão:
170221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


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[PMID]:27552542
[Au] Autor:Pearce SH; Razvi S; Yadegarfar ME; Martin-Ruiz C; Kingston A; Collerton J; Visser TJ; Kirkwood TB; Jagger C
[Ad] Endereço:Institute of Genetic Medicine (S.H.S.P., S.R.), Newcastle University, Newcastle upon Tyne NE1 3BZ, United Kingdom; Institute of Health and Society (M.E.Y., C.M.-R., A.K., J.C., T.B.K., C.J.), Newcastle University, Newcastle upon Tyne NE2 4AX, United Kingdom; and Department of Internal Medicine (T.J.
[Ti] Título:Serum Thyroid Function, Mortality and Disability in Advanced Old Age: The Newcastle 85+ Study.
[So] Source:J Clin Endocrinol Metab;101(11):4385-4394, 2016 Nov.
[Is] ISSN:1945-7197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Perturbations in thyroid function are common in older individuals but their significance in the very old is not fully understood. OBJECTIVE: This study sought to determine whether thyroid hormone status and variation of thyroid hormones within the reference range correlated with mortality and disability in a cohort of 85-year-olds. DESIGN: A cohort of 85-year-old individuals were assessed in their own homes (community or institutional care) for health status and thyroid function, and followed for mortality and disability for up to 9 years. SETTING AND PARTICIPANTS: Six hundred and forty-three 85-year-olds registered with participating general practices in Newcastle and North Tyneside, United Kingdom. MAIN OUTCOMES: All-cause mortality, cardiovascular mortality, and disability according to thyroid disease status and baseline thyroid hormone parameters (serum TSH, FT , FT , and rT ). Models were adjusted for age, sex, education, body mass index, smoking, and disease count. RESULTS: After adjustment for age and sex, all-cause mortality was associated with baseline serum rT and FT (both P < .001), but not FT or TSH. After additional adjustment for potential confounders, only rT remained significantly associated with mortality (P = .001). Baseline serum TSH and rT predicted future disability trajectories in men and women, respectively. CONCLUSIONS: Our study is reassuring that individuals age 85 y with both subclinical hypothyroidism and subclinical hyperthyroidism do not have a significantly worse survival over 9 years than their euthyroid peers. However, thyroid function tests did predict disability, with higher serum TSH levels predicting better outcomes. These data strengthen the argument for routine use of age-specific thyroid function reference ranges.
[Mh] Termos MeSH primário: Doenças Cardiovasculares/mortalidade
Pessoas com Deficiência/estatística & dados numéricos
Mortalidade
Doenças da Glândula Tireoide/sangue
Doenças da Glândula Tireoide/epidemiologia
Tireotropina/sangue
Tri-Iodotironina Reversa/sangue
[Mh] Termos MeSH secundário: Idoso de 80 Anos ou mais
Dextrotireoxina/sangue
Inglaterra/epidemiologia
Feminino
Seres Humanos
Estudos Longitudinais
Masculino
Valores de Referência
Testes de Função Tireóidea
Tri-Iodotironina/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
06LU7C9H1V (Triiodothyronine); 4W9K63FION (Dextrothyroxine); 5817-39-0 (Triiodothyronine, Reverse); 9002-71-5 (Thyrotropin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160824
[St] Status:MEDLINE


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[PMID]:26947333
[Au] Autor:Chen K; Yan B; Wang F; Wen F; Xing X; Tang X; Shi Y; Le G
[Ad] Endereço:The Laboratory of Food Nutrition and Functional Factors, Food Science and Technology, Jiangnan University, Wuxi 214122, China.
[Ti] Título:Type 1 5'-deiodinase activity is inhibited by oxidative stress and restored by alpha-lipoic acid in HepG2 cells.
[So] Source:Biochem Biophys Res Commun;472(3):496-501, 2016 Apr 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:3,3',5-triiodothyronine (T3) is largely generated from thyroxine (T4) by the catalysis of deiodinases in peripheral tissues. Emerging evidences have indicated its broad participation in regulating various metabolic process via protecting tissues from oxidative stress and improving cellular antioxidant capacity. However, the potential correlation between the oxidative stress and conversion of T4 to T3 is still unclear. In the present study, the effects of T3 and T4 on redox homeostasis in HepG2 cells pre-treated with H2O2 was investigated. It revealed that T3 significantly rescued the apoptotic cell death, consistent with an upregulation of cell antioxidant ability and reduction of ROS accumulation while T4 did not. Afterwards, we examined the enzyme activity and mRNA expression of type 1 5'-deiodianse (DIO1), T3 and rT3 level and found that H2O2 reduced both DIO1 activity and expression in a dose-dependent manner, which consequently declined T3 and rT3 generation. Alpha-lipoic acid (LA) treatment notably restored DIO1 activity, T3 and rT3 level, as well as transcriptional abnormalities of inflammation-associated genes. It suggests that oxidative stress may reduce DIO1 activity by an indirect way like activating cellular inflammatory responses. All these results indicate that the oxidative stress downregulates the conversion of T4 to T3 through DIO1 function in HepG2 cells.
[Mh] Termos MeSH primário: Iodeto Peroxidase/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Estresse Oxidativo/fisiologia
Ácido Tióctico/administração & dosagem
Tiroxina/metabolismo
Tri-Iodotironina Reversa/metabolismo
[Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga
Ativação Enzimática/efeitos dos fármacos
Células Hep G2
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
5817-39-0 (Triiodothyronine, Reverse); 73Y7P0K73Y (Thioctic Acid); EC 1.11.1.- (iodothyronine deiodinase type I); EC 1.11.1.8 (Iodide Peroxidase); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160325
[Lr] Data última revisão:
160325
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160308
[St] Status:MEDLINE


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[PMID]:26049370
[Au] Autor:Moura Neto A; Parisi MC; Alegre SM; Pavin EJ; Tambascia MA; Zantut-Wittmann DE
[Ad] Endereço:Division of Endocrinology, Clinical Medicine Department, Faculty of Medical Sciences, University of Campinas, UNICAMP, Rua Tessalia Vieira de Camargo, 126 - Barao Geraldo, CEP 13084-971, Campinas, Sao Paulo, Brazil.
[Ti] Título:Relation of thyroid hormone abnormalities with subclinical inflammatory activity in patients with type 1 and type 2 diabetes mellitus.
[So] Source:Endocrine;51(1):63-71, 2016 Jan.
[Is] ISSN:1559-0100
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Thyroid hormone (TH) abnormalities are common in patients with diabetes mellitus (DM). These thyroid hormone abnormalities have been associated with inflammatory activity in several conditions but this link remains unclear in DM. We assessed the influence of subclinical inflammation in TH metabolism in euthyroid diabetic patients. Cross-sectional study involving 258 subjects divided in 4 groups: 70 patients with T2DM and 55 patients with T1DM and two control groups of 70 and 63 non-diabetic individuals, respectively. Groups were paired by age, sex, and body mass index (BMI). We evaluated the association between clinical and hormonal variables [thyrotropin, reverse T3 (rT3), total and free thyroxine (T4), and triiodothyronine (T3)] with the inflammation markers C-reactive protein (hs-CRP), serum amyloid A (SAA), and interleukin-6 (IL-6). Serum T3 and free T3 were lower in patients with diabetes (all P < 0.001) compared to the control groups. Interleukin-6 showed positive correlations with rT3 in both groups (P < 0.05). IL-6 was independently associated to FT3/rT3 (B = -0.193; 95% CI -0.31; -0.076; P = 0.002) and FT4/rT3 (B = -0.107; 95% CI -0.207; -0.006; P = 0.039) in the T1DM group. In the T2DM group, SAA (B = 0.18; 95% CI 0.089; 0.271; P < 0.001) and hs-CRP (B = -0.069; 95% CI -0.132; -0.007; P = 0.03) predicted FT3 levels. SAA (B = -0.16; 95% CI -0.26; -0.061; P = 0.002) and IL6 (B = 0.123; 95% CI 0.005; 0.241; P = 0.041) were related to FT4/FT3. In DM, differences in TH levels compared to non-diabetic individuals were related to increased subclinical inflammatory activity and BMI. Altered deiodinase activity was probably involved. These findings were independent of sex, age, BMI, and HbA1c levels.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 1/complicações
Diabetes Mellitus Tipo 2/complicações
Inflamação/complicações
Doenças da Glândula Tireoide/complicações
Hormônios Tireóideos/sangue
[Mh] Termos MeSH secundário: Adulto
Doenças Assintomáticas
Proteína C-Reativa/metabolismo
Estudos de Casos e Controles
Estudos Transversais
Diabetes Mellitus Tipo 1/sangue
Diabetes Mellitus Tipo 1/epidemiologia
Diabetes Mellitus Tipo 2/sangue
Diabetes Mellitus Tipo 2/epidemiologia
Feminino
Seres Humanos
Inflamação/sangue
Inflamação/epidemiologia
Interleucina-6/sangue
Masculino
Meia-Idade
Proteína Amiloide A Sérica/metabolismo
Doenças da Glândula Tireoide/sangue
Doenças da Glândula Tireoide/epidemiologia
Testes de Função Tireóidea
Tri-Iodotironina Reversa/sangue
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (IL6 protein, human); 0 (Interleukin-6); 0 (Serum Amyloid A Protein); 0 (Thyroid Hormones); 5817-39-0 (Triiodothyronine, Reverse); 9007-41-4 (C-Reactive Protein)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150608
[St] Status:MEDLINE
[do] DOI:10.1007/s12020-015-0651-5


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[PMID]:26426328
[Au] Autor:Sakai H; Nagao H; Sakurai M; Okumura T; Nagai Y; Shikuma J; Ito R; Imazu T; Miwa T; Odawara M
[Ad] Endereço:Department of Diabetes, Endocrinology and Metabolism, Tokyo Medical University, Tokyo, Japan.
[Ti] Título:Correlation between Serum Levels of 3,3',5'-Triiodothyronine and Thyroid Hormones Measured by Liquid Chromatography-Tandem Mass Spectrometry and Immunoassay.
[So] Source:PLoS One;10(10):e0138864, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: For measuring serum 3,3',5'-triiodothyronine (rT3) levels, radioimmunoassay (RIA) has traditionally been used owing to the lack of other reliable methods; however, it has recently become difficult to perform. Meanwhile, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has recently been attracting attention as a novel alternative method in clinical chemistry. To the best of our knowledge, there are no studies to date comparing results of the quantification of human serum rT3 between LC-MS/MS and RIA. We therefore examined the feasibility of LC-MS/MS as a novel alternative method for measuring serum rT3, thyroxine (T4), and 3,5,3'-triiodothyronine (T3) levels. METHODS: Assay validation was performed by LC-MS/MS using quality control samples of rT3, T4, and T3 at 4 various concentrations which were prepared from reference compounds. Serum samples of 50 outpatients in our department were quantified both by LC-MS/MS and conventional immunoassay for rT3, T4, and T3. Correlation coefficients between the 2 measurement methods were statistically analyzed respectively. RESULTS: Matrix effects were not observed with our method. Intra-day and inter-day precisions were less than 10.8% and 9.6% for each analyte at each quality control level, respectively. Intra-day and inter-day accuracies were between 96.2% and 110%, and between 98.3% and 108.6%, respectively. The lower limit of quantification was 0.05 ng/mL. Strong correlations were observed between the 2 measurement methods (correlation coefficient, T4: 0.976, p < 0.001; T3: 0.912, p < 0.001; rT3: 0.928, p < 0.001). CONCLUSIONS: Our LC-MS/MS system requires no manual cleanup operation, and the process after application of a sample is fully automated; furthermore, it was found to be highly sensitive, and superior in both precision and accuracy. The correlation between the 2 methods over a wide range of concentrations was strong. LC-MS/MS is therefore expected to become a useful tool for clinical diagnosis and research.
[Mh] Termos MeSH primário: Análise Química do Sangue/métodos
Cromatografia Líquida/métodos
Radioimunoensaio/métodos
Espectrometria de Massas em Tandem/métodos
Tri-Iodotironina Reversa/sangue
[Mh] Termos MeSH secundário: Estudos de Viabilidade
Feminino
Seres Humanos
Modelos Lineares
Masculino
Meia-Idade
Reprodutibilidade dos Testes
Tiroxina/sangue
Tri-Iodotironina/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
06LU7C9H1V (Triiodothyronine); 5817-39-0 (Triiodothyronine, Reverse); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151002
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0138864


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[PMID]:25804834
[Au] Autor:Kahl S; Elsasser TH; Rhoads RP; Collier RJ; Baumgard LH
[Ad] Endereço:U.S. Department of Agriculture, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA. Electronic address: stanislaw.kahl@ars.usda.gov.
[Ti] Título:Environmental heat stress modulates thyroid status and its response to repeated endotoxin challenge in steers.
[So] Source:Domest Anim Endocrinol;52:43-50, 2015 Jul.
[Is] ISSN:1879-0054
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to evaluate in cattle, the effects of acute exposure to a heat stress (HS) environment on the status of the pituitary (thyrotropin, TSH)-thyroid (thyroxine, T4)-peripheral tissue T4 deiodination (type 1 5'-deiodinase [D1]; triiodothyronine [T3]; reverse-triiodothyronine [rT3]) axis, and the further response of this pituitary-thyroid-peripheral tissue axis (PTTA) to perturbation caused by the induction of the proinflammatory innate immune state provoked by the administration of gram-negative bacteria endotoxin (lipopolysaccharide [LPS]). Ten steers (318 ± 49 kg body weight) housed in controlled environment chambers were subjected to either a thermoneutral (TN: constant 19°C) or HS temperature conditions (cyclical daily temperatures: 32.2°C-40.0°C) for a total period of 9 d. To minimize the effects of altered plane of nutrition due to HS, steers in TN were pair-fed to animals in HS conditions. Steers received 2 LPS challenges 3 d apart (LPS1 and LPS2; 0.2 µg/kg body weight, intravenously, Escherichia coli 055:B5) with the first challenge administered on day 4 relative to the start of the environmental conditioning. Jugular blood samples were collected at 0, 1, 2, 4, 7, and 24 h relative to the start of each LPS challenge. Plasma TSH, T4, T3, and rT3 were measured by radioimmunoassay. Liver D1 activity was measured in biopsy samples collected before the LPS1 (0 h) and 24 h after LPS2. Before the start of LPS1, HS decreased (P < 0.01 vs TN) plasma TSH (40%), T4 (45.4%), and T3 (25.9%), but did not affect rT3 concentrations. In TN steers, the LPS1 challenge decreased (P < 0.01 vs 0 h) plasma concentrations of TSH between 1 and 7 h and T4 and T3 at 7 and 24 h. In HS steers, plasma TSH concentrations were decreased at 2 h only (P < 0.05), whereas plasma T3 was decreased at 7 and 24 h (P < 0.01). Whereas plasma T4 concentrations were already depressed in HS steers at 0 h, LPS1 did not further affect the levels. Plasma rT3 concentrations were increased in all steers at 4, 7, and 24 h after LPS1 (P < 0.01). The patterns of concentration change of T4, T3, and rT3 during LPS2 mirrored those observed in LPS1; the responses in plasma TSH were of smaller magnitude than those incurred after LPS1. The LPS challenges reduced (P < 0.01) hepatic activity of D1 in all animals but no differences were observed between steers subjected to TN or HS environment. The data are consistent with the concept that acute exposure of cattle to a HS environment results in the depression of the pituitary and thyroid components of the PTTA, whereas a normal capacity to generate T3 from T4 in the liver is preserved. The data also suggest that LPS challenge further suppresses all components of the PTTA including liver T3 generation, and these PTTA perturbations are more pronounced in steers that encounter a HS exposure.
[Mh] Termos MeSH primário: Bovinos/fisiologia
Temperatura Alta
Lipopolissacarídeos/farmacologia
Estresse Fisiológico
Glândula Tireoide/fisiologia
[Mh] Termos MeSH secundário: Animais
Iodeto Peroxidase/metabolismo
Fígado/enzimologia
Fígado/metabolismo
Masculino
Hipófise/fisiologia
Proteína Amiloide A Sérica/análise
Estresse Fisiológico/imunologia
Estresse Fisiológico/fisiologia
Tireotropina/sangue
Tiroxina/sangue
Tri-Iodotironina/sangue
Tri-Iodotironina Reversa/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipopolysaccharides); 0 (Serum Amyloid A Protein); 06LU7C9H1V (Triiodothyronine); 5817-39-0 (Triiodothyronine, Reverse); 9002-71-5 (Thyrotropin); EC 1.11.1.- (iodothyronine deiodinase type I); EC 1.11.1.8 (Iodide Peroxidase); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150606
[Lr] Data última revisão:
150606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150326
[St] Status:MEDLINE


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[PMID]:25549541
[Au] Autor:Venkatesh K; Srikanth L; Vengamma B; Chandrasekhar C; Prasad BC; Sarma PV
[Ad] Endereço:Stem cell Laboratory, Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, 517507, India.
[Ti] Título:In vitro transdifferentiation of human cultured CD34+ stem cells into oligodendrocyte precursors using thyroid hormones.
[So] Source:Neurosci Lett;588:36-41, 2015 Feb 19.
[Is] ISSN:1872-7972
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:The extent of myelination on the axon promotes transmission of impulses in the neural network, any disturbances in this process results in the neurodegenerative condition. Transplantation of oligodendrocyte precursors that supports in the regeneration of axons through myelination is an important step in the restoration of damaged neurons. Therefore, in the present study, the differentiation of human CD34+ stem cells into oligodendrocytes was carried out. The pure human CD34+ culture developed from the stem cells obtained from a peripheral blood of a donor were subjected to oligodendrocyte differentiation medium (ODM). The ODM at a concentration of 40ng/ml thyroxine, 40ng/ml 3,3',5-tri-iodo-thyronine showed distinct morphological changes from day 6 to 9 with cells exhibiting conspicuous stellate morphology and extensive foot processes. The real-time PCR analysis showed prominent expression of Olig2, CNPase, PDGFRα and PLP1/DM20 in the differentiated cells confirming the formed cells are oligodendrocyte precursors. The expression of these genes increased from days 6 to 9 corresponding to the morphological changes observed with almost no expression of GFAP+ cells. The distinct CNPase activity was observed in these differentiated cells compared to normal CD34+ stem cells correlating with results of real-time PCR conclusively explains the development of oligodendrocytes from human CD34+ stem cells.
[Mh] Termos MeSH primário: Antígenos CD34/metabolismo
Transdiferenciação Celular
Células-Tronco Neurais/efeitos dos fármacos
Oligodendroglia/efeitos dos fármacos
Tiroxina/farmacologia
Tri-Iodotironina Reversa/farmacologia
[Mh] Termos MeSH secundário: 2',3'-Nucleotídeo Cíclico Fosfodiesterases/metabolismo
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Células Cultivadas
Meios de Cultura
Seres Humanos
Masculino
Proteína Proteolipídica de Mielina/metabolismo
Proteínas do Tecido Nervoso/metabolismo
Células-Tronco Neurais/citologia
Células-Tronco Neurais/metabolismo
Fator de Transcrição 2 de Oligodendrócitos
Oligodendroglia/citologia
Oligodendroglia/metabolismo
Receptor alfa de Fator de Crescimento Derivado de Plaquetas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD34); 0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Culture Media); 0 (Myelin Proteolipid Protein); 0 (Nerve Tissue Proteins); 0 (OLIG2 protein, human); 0 (Oligodendrocyte Transcription Factor 2); 0 (PLP1 protein, human); 5817-39-0 (Triiodothyronine, Reverse); EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor alpha); EC 3.1.4.- (2',3'-Cyclic-Nucleotide Phosphodiesterases); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150101
[St] Status:MEDLINE


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[PMID]:25241124
[Au] Autor:Akiyama S; Ogiwara T; Aoki T; Tsunekawa K; Araki O; Murakami M
[Ad] Endereço:Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
[Ti] Título:Glucagon-like peptide-1 stimulates type 3 iodothyronine deiodinase expression in a mouse insulinoma cell line.
[So] Source:Life Sci;115(1-2):22-8, 2014 Oct 12.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: The pathophysiological roles of thyroid hormones in glucose metabolism remain uncertain. Type 3 iodothyronine deiodinase (D3) converts thyroxine (T4) and 3,5,3'-triiodothyronine (T3) to 3,3',5'-triiodothyronine (rT3) and 3,3'-diiodothyronine (T2), respectively, inactivating thyroid hormones in a cell-specific fashion. In the present study, we identified D3 expression in MIN6 cells derived from a mouse insulinoma cell line and examined the mechanisms regulating D3 expression in these cells. MAIN METHODS: We characterized D3 activity using HPLC analysis, and examined the effect of GLP-1 or exendin-4 on D3 expression and cAMP accumulation in MIN6 cells. We also measured insulin secretion from MIN6 cells exposed to GLP-1 and T3. KEY FINDINGS: We identified enzyme activity that catalyzes the conversion of T3 to T2 in MIN6 cells, which showed characteristics compatible with those for D3. D3 mRNA was identified in these cells using RT-PCR analysis. Forskolin rapidly stimulated D3 mRNA and D3 activity. Glucagon-like peptide-1 (GLP-1) increased D3 expression in a dose-dependent manner, and this effect was inhibited by the protein kinase A (PKA) inhibitor H-89. Exendin-4, a GLP-1 receptor agonist, also stimulated D3 expression in MIN6 cells. These results suggest that a cAMP-PKA-mediated pathway participates in GLP-1-stimulated D3 expression in MIN6 cells. Furthermore, GLP-1 stimulated insulin secretion was suppressed by the addition of T3 in MIN6 cells. SIGNIFICANCE: Our findings indicate that GLP-1 regulates intracellular T3 concentration in pancreatic ß cells via a cAMP-PKA-D3-mediated pathway that may also regulate ß-cell function.
[Mh] Termos MeSH primário: Regulação Neoplásica da Expressão Gênica
Peptídeo 1 Semelhante ao Glucagon/metabolismo
Células Secretoras de Insulina/metabolismo
Insulinoma/metabolismo
Iodeto Peroxidase/metabolismo
Neoplasias Pancreáticas/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Colforsina/farmacologia
AMP Cíclico/metabolismo
Proteínas Quinases Dependentes de AMP Cíclico/antagonistas & inibidores
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Células Secretoras de Insulina/patologia
Insulinoma/genética
Insulinoma/patologia
Iodeto Peroxidase/genética
Camundongos
Neoplasias Pancreáticas/genética
Neoplasias Pancreáticas/patologia
Peptídeos/farmacologia
RNA Mensageiro/genética
Transdução de Sinais
Tri-Iodotironina/metabolismo
Tri-Iodotironina Reversa/metabolismo
Peçonhas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Peptides); 0 (RNA, Messenger); 0 (Venoms); 06LU7C9H1V (Triiodothyronine); 1F7A44V6OU (Colforsin); 5817-39-0 (Triiodothyronine, Reverse); 89750-14-1 (Glucagon-Like Peptide 1); 9P1872D4OL (exenatide); E0399OZS9N (Cyclic AMP); EC 1.11.1.- (iodothyronine deiodinase type III); EC 1.11.1.8 (Iodide Peroxidase); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:141006
[Lr] Data última revisão:
141006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140922
[St] Status:MEDLINE


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[PMID]:24932603
[Au] Autor:Lobotková D; Staníková D; Staník J; Cervenová O; Bzdúch V; Tichá L
[Ad] Endereço:Comenius University Faculty of Medicine and Children's University Hospital, Department of Pediatrics, Bratislava, Slovakia. E-ma-il: ticha.lubica@gmail.com.
[Ti] Título:Lack of association between peripheral activity of thyroid hormones and elevated TSH levels in childhood obesity.
[So] Source:J Clin Res Pediatr Endocrinol;6(2):100-4, 2014.
[Is] ISSN:1308-5735
[Cp] País de publicação:Turkey
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: An elevated thyroid stimulating hormone (TSH) level is a frequent finding in obese children, but its association with peripheral hormone metabolism is not fully understood. We hypothesized that in obesity, the changes in thyroid hormone metabolism in peripheral tissues might lead to dysregulation in the thyroid axis. The purpose of this study was to investigate the association of TSH with thyroid hormones in a group of obese children as compared to normal-weight controls. METHODS: Serum TSH, free thyroxine (fT4) and free triiodothyronine (fT3) levels were measured in 101 obese children and in 40 controls. Serum reverse T3 (rT3) levels were also measured in a subgroup of 51 obese children and in 15 controls. RESULTS: Serum TSH level was significantly higher in obese children compared to controls (2.78 vs. 1.99 mIU/L, p<0.001), while no difference was found in fT4, fT3, rT3 levels and in fT3/rT3 ratio. In the obese group, fT3 level positively correlated with fT4 (r=0.217, p=0.033) and inversely with rT3 (r=-0.288, p=0.045). However, thyroid hormone levels and TSH levels were not correlated. CONCLUSION: In obese children, normal fT4, fT3 and rT3 levels suggest an undisturbed peripheral hormone metabolism. These levels show no correlation with elevated TSH levels.
[Mh] Termos MeSH primário: Obesidade Pediátrica/sangue
Hormônios Tireóideos/sangue
Tireotropina/sangue
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
Feminino
Seres Humanos
Masculino
Tiroxina/sangue
Tri-Iodotironina/sangue
Tri-Iodotironina Reversa/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Thyroid Hormones); 06LU7C9H1V (Triiodothyronine); 5817-39-0 (Triiodothyronine, Reverse); 9002-71-5 (Thyrotropin); Q51BO43MG4 (Thyroxine)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140617
[St] Status:MEDLINE
[do] DOI:10.4274/Jcrpe.1251



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