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[PMID]:28963084
[Au] Autor:Bryja J; Kostin D; Meheretu Y; Sumbera R; Bryjová A; Kasso M; Mikula O; Lavrenchenko LA
[Ad] Endereço:Institute of Vertebrate Biology of the Czech Academy of Sciences, Brno, Czech Republic; Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic. Electronic address: bryja@brno.cas.cz.
[Ti] Título:Reticulate Pleistocene evolution of Ethiopian rodent genus along remarkable altitudinal gradient.
[So] Source:Mol Phylogenet Evol;118:75-87, 2018 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Ethiopian highlands are the most extensive complex of mountainous habitats in Africa. The presence of the Great Rift Valley (GRV) and the striking elevational ecological gradients inhabited by recently radiated Ethiopian endemics, provide a wide spectrum of model situations for evolutionary studies. The extant species of endemic rodents, often markedly phenotypically differentiated, are expected to possess complex genetic features which evolved asa consequence of the interplay between geomorphology and past climatic changes. In this study, we used the largest available multi-locus genetic dataset of the murid genus Stenocephalemys (347 specimens from ca 40 localities across the known distributional area of all taxa) to investigate the relative importance of disruptive selection, temporary geographic isolation and introgression in their adaptive radiations in the Pleistocene. We confirmed the four main highly supported mitochondrial (mtDNA) clades that were proposed as four species in a previous pilot study: S. albipes is a sister species of S. griseicauda (both lineages are present on both sides of the GRV), while the second clade is formed by two Afro-alpine species, S. albocaudata (east of GRV) and the undescribed Stenocephalemys sp. A (west of GRV). There is a clear elevational gradient in the distribution of the Stenocephalemys taxa with two to three species present at different elevations of the same mountain range. Surprisingly, the nuclear species tree corresponded only a little to the mtDNA tree. Multispecies coalescent models based on six nuclear markers revealed the presence of six separate gene pools (i.e. candidate species), with different topology. Phylogenetic analysis, together with the geographic distribution of the genetic groups, suggests a complex reticulate evolution. We propose a scenario that involves (besides classical allopatric speciation) two cases of disruptive selection along the elevational ecological gradient, multiple crosses of GRV in dry and cold periods of the Pleistocene, followed by hybridization and mtDNA introgression on imperfect reproductive barriers. Spatial expansion of the currently most widespread "albipes" mtDNA clade was followed by population fragmentation, lineage sorting and again by hybridization and mtDNA introgression. Comparison of this genetic structure to other Ethiopian endemic taxa highlight the geographical areas of special conservation concern, where more detailed biodiversity studies should be carried out to prevent many endemic taxa from going extinct even before they are recognized.
[Mh] Termos MeSH primário: Evolução Molecular
Murinae/classificação
[Mh] Termos MeSH secundário: Animais
Citocromos b/química
Citocromos b/classificação
Citocromos b/genética
DNA Mitocondrial/química
DNA Mitocondrial/isolamento & purificação
DNA Mitocondrial/metabolismo
Ecossistema
Etiópia
Haplótipos
Hibridização Genética
Cariótipo
Murinae/anatomia & histologia
Murinae/genética
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 9035-37-4 (Cytochromes b)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171001
[St] Status:MEDLINE


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[PMID]:28919506
[Au] Autor:Gonçalves DV; Martínez-Freiría F; Crochet PA; Geniez P; Carranza S; Brito JC
[Ad] Endereço:CIBIO/InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal; Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua Campo Alegre, 4169-007 Porto, Portugal; Institute of Evolutionary Biolog
[Ti] Título:The role of climatic cycles and trans-Saharan migration corridors in species diversification: Biogeography of Psammophis schokari group in North Africa.
[So] Source:Mol Phylogenet Evol;118:64-74, 2018 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Highlands, hydrographic systems and coastal areas have been hypothesised to form corridors across the hyperarid Sahara desert in North Africa, allowing dispersal and gene flow for non-xeric species. Here we aim to provide a genetic test for the trans-Saharan corridor model, and predict the location and stability of ecological-corridors, by combining phylogeography and palaeoclimatic modelling. The model was the Psammophis schokari (Schokari sand racer) group, fast-moving and widely distributed generalist colubrids occurring mostly in arid and semiarid scrublands. We combined dated phylogenies of mitochondrial and nuclear markers with palaeoclimatic modelling. For the phylogeographic analysis, we used 75 samples of P. schokari and P. aegyptius, and Bayesian and Maximum-Likelihood methods. For the ecological models, we used Maxent over the distribution of P. schokari and West African lineages. Models were projected to past conditions (mid Holocene, Last Glacial Maximum and Last Inter-Glacial) to infer climatic stable areas. Climatic stability was predicted to be mostly restricted to coastal areas and not spatially continuous. A putative temporary trans-Saharan corridor was identified in Eastern Sahara, with a more stable one along the Atlantic coast. Six parapatric lineages were identified within P. schokari, four occurring in North Africa. These likely diverged during the Pliocene. The Tamanraset River might have been a vicariant agent. African lineages may have experienced further subsequent diversification during the late Pleistocene. The main P. schokari refugia were probably located along the northern margins of the Sahara, allowing its North-to-South colonization. Trans-Saharan corridors seem to have played a role in P. schokari biogeography, allowing colonization of central Saharan mountains and Sahel. Some might have worked as refugia, and even the most stable corridors may have sections working as filters, depending on each climatic phase. We expect the use of trans-Saharan corridors to decrease for more mesic species or with less dispersal capabilities.
[Mh] Termos MeSH primário: Serpentes/classificação
[Mh] Termos MeSH secundário: África do Norte
Migração Animal
Animais
Teorema de Bayes
Clima
Citocromos b/química
Citocromos b/genética
DNA Mitocondrial/química
DNA Mitocondrial/isolamento & purificação
DNA Mitocondrial/metabolismo
Variação Genética
Funções Verossimilhança
NADH Desidrogenase/química
NADH Desidrogenase/genética
Filogenia
Filogeografia
Análise de Sequência de DNA
Serpentes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 9035-37-4 (Cytochromes b); EC 1.6.99.3 (NADH Dehydrogenase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE


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[PMID]:29372806
[Au] Autor:Slynko YV; Slynko EE; Karpova EP; Boltachev AR
[Ti] Título:[Genetic variation of the mtDNA cyt b locus in topmouth gudgeon introduced into water bodies in the northern part of the Black Sea region].
[So] Source:Genetika;53(1):79-87, 2017 Jan.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The up-to-date phylogeographical distribution of the topmouth gudgeon Pseudorasbora parva Temminck et Schlegel 1846 in water bodies of the Northern Black Sea region is considered. Genetic variation of mtDNA cyt b gene is analyzed. It is established that topmouth gudgeon penetrated and spread in the basins of the Dnieper and Don rivers and in water bodies of Crimea from the secondary center of its dispersion­ water bodies of Central Europe. It is demonstrated that haplotypes of topmouth gudgeon in the Danube delta are the most homologous to the haplotypes in the native range of the species in China. A considerable decrease in the level of genetic variation in the populations in the Black Sea region is reported.
[Mh] Termos MeSH primário: Cyprinidae/genética
Citocromos b/genética
DNA Mitocondrial/genética
Proteínas de Peixes/genética
Variação Genética
Proteínas Mitocondriais/genética
[Mh] Termos MeSH secundário: Animais
Mar Negro
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (Fish Proteins); 0 (Mitochondrial Proteins); 9035-37-4 (Cytochromes b)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE


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[PMID]:29372798
[Au] Autor:Soshina VA; Pavlov SD; Zelenina DA
[Ti] Título:[Genetic diversity of charrs of the Commander Islands based on the analysis of mitochondrial DNA].
[So] Source:Genetika;52(11):1336-41, 2016 Nov.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Nucleotide sequences of the mitochondrial DNA cytochrome b (CytB) gene fragment and the control region (D-loop) of Dolly Varden (Salvelinus malma) from the Commander Islands and the Kol River of the Kamchatka Peninsula were examined. A high level of genetic variability of island populations comparable to that of the mainland population of western Kamchatka was demonstrated. The belonging of the Commander Islands chars to the genetic lineage of northern Dolly Varden Salvelinus malma malma was confirmed.
[Mh] Termos MeSH primário: Citocromos b/genética
Proteínas de Peixes/genética
Variação Genética
Salmonidae/genética
[Mh] Termos MeSH secundário: Animais
Sibéria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 9035-37-4 (Cytochromes b)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE


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[PMID]:29369561
[Au] Autor:Spiridonova LN; Valchuk OP; Red'kin YA; Kryukov AP
[Ti] Título:[Nuclear mtDNA pseudogenes as a source of new variants of the mtDNA cytochrome b haplotypes: A case study of Siberian rubythroat Luscinia calliope (Muscicapidae, Aves)].
[So] Source:Genetika;52(9):1069-80, 2016 Sep.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Sequence polymorphism of the mitochondrial DNA cytochrome b gene fragment was analyzed in 21 specimens of subspecies Luscinia calliope calliope (Pallas, 1776) and two specimens of L. c. anadyrensis (Portenko, 1939). On sequence chromatograms, in 19 specimens of L. c. calliope, double peaks of heteroplasmy type in the taxon-specific positions were revealed. Moreover, two clone variants were identified. The first variant was the calliope mitochondrial cyt b gene and the second was the nuclear cyt b pseudogene, similar to the mitochondrial haplotype anadyrensis-camtschatkensis. In L. c. anadyrensis, four clone variants, represented by the mitochondrial calliope and anadyrensis-camtschatkensis cyt b genes and nuclear calliope and sachalinensis cyt b pseudogenes, were identified. Some nuclear cyt b pseudogenes were highly similar (98­99%) to the mitochondrial genes of the subspecies L. c. anadyrensis, L. c. camtschatkensis, and L. c. sachalinensis. In the same time, the majority of nuclear pseudogene sequences were characterized by a high level of polymorphism, caused by nonsynonymous substitutions (up to five substitutions per sequence), the presence of indels in some of the clones, and TAA and TGA stop codons. In our opinion, the mitochondrial haplotypes anadyrensis-camtschatkensis and sachalinensis occurred as a result of intergenomic homologous recombination. This finding provides a new insight into the colonization history of the northeastern part of the range by L. calliope, according to which populating the territory of Chukotka, Kamchatka, and Sakhalin took place at different times and along the independent pathways.
[Mh] Termos MeSH primário: Proteínas Aviárias/genética
Citocromos b/genética
Genes Mitocondriais
Passeriformes/genética
Pseudogenes
[Mh] Termos MeSH secundário: Animais
Sibéria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Avian Proteins); 9035-37-4 (Cytochromes b)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


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[PMID]:29368910
[Au] Autor:Sinyov VV; Chicheva MM; Barinova VA; Ryzhkova AI; Zilinyi RI; Karagodin VP; Postnov AY; Sobenin IA; Orekhov AN; Sazonova MA
[Ti] Título:[The heteroplasmy level of some mutations in gene MT-CYB among women with asymptomatic atherosclerosis].
[So] Source:Genetika;52(8):951-7, 2016 Aug.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Atherosclerosis is a polygenic socially significant disease whose risk factors include coronary heart disease, diabetes, hypertension, and myocardial infarction. According to the literature, mutations m.14846G>A (G34S), m.15762G>A (G339Q), m.15084G>A (W113Ter), and m.15059G>A (G190Ter) of cytochrome B gene (MT-CYB) are associated with mitochondrial myopathies, myoglobinuria, and exercise intolerance. Preliminary studies carried out by the authors made it possible to discover an association of certain mitochondrial genome mutations with atherosclerotic lesions of aortic intima in people who died as a result of an accident or sudden death. The most interesting seemed to be the data on the association of mutations m.14846G>A and m.15059G>A of the cytochrome B gene with lipofibrous aortic plaques, because these mutations affect the mitochondrial respiratory chain enzyme. Defects in the given chain may be the reason for the launch of pathogenic mechanisms in the human body. Owing to the fact that mutations in the mitochondrial genome are inherited by the maternal type, it was decided to analyze cytochrome B gene mutations in a sample of female volunteers from Moscow oblast. According to the findings, mutations m.14846G>A and m.15059G>A are highly significantly associated with atherosclerotic lesions of the carotid arteries: m.14846G>A is antiatherogenic and m.15059G>A is proatherogenic.
[Mh] Termos MeSH primário: Aterosclerose/genética
Doenças das Artérias Carótidas/genética
Citocromos b/genética
Mutação
Placa Aterosclerótica/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Aterosclerose/enzimologia
Doenças das Artérias Carótidas/enzimologia
Feminino
Seres Humanos
Meia-Idade
Placa Aterosclerótica/enzimologia
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
9035-37-4 (Cytochromes b)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


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[PMID]:29227999
[Au] Autor:Buj I; Marcic Z; Caleta M; Sanda R; Geiger MF; Freyhof J; Machordom A; Vukic J
[Ad] Endereço:Division of Zoology, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
[Ti] Título:Ancient connections among the European rivers and watersheds revealed from the evolutionary history of the genus Telestes (Actinopterygii; Cypriniformes).
[So] Source:PLoS One;12(12):e0187366, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In order to better understand the complex geologic history of the Mediterranean area, we have analysed evolutionary history, phylogeographic structure and molecular diversity of freshwater fishes belonging to the genus Telestes. As primary freshwater fishes distributed largely in the Mediterranean basin, this genus represents a suitable model system for investigating the historical biogeography of freshwater drainage systems in southern Europe. In this investigation we have included samples representing all Telestes species and based our analyses on one mitochondrial and one nuclear gene. We have investigated phylogenetic structure inside the genus Telestes, estimated divergence times, reconstructed ancestral distribution ranges and described intraspecific molecular diversity. Diversification of Telestes started in the Early Miocene, when the ancestors of T. souffia, lineage comprising T. croaticus and T. fontinalis, and the one comprising T. pleurobipunctatus and T. beoticus got isolated. The remaining species are genetically more closely related and form a common cluster in the recovered phylogenetic trees. Complex geological history of southern Europe, including formation of continental bridges, fragmentation of landmass, closing of the sea corridor, local tectonic activities, led to complicated biogeographical pattern of this genus, caused by multiple colonization events and passovers between ancient rivers and water basins. Especially pronounced diversity of Telestes found in the Adriatic watershed in Croatia and Bosnia and Herzegovina is a consequence of a triple colonization of this area by different lineages, which led to an existence of genetically distinct species in neighboring areas. Significant intraspecific structuring is present in T. souffia, T. muticellus, T. croaticus and T. pleurobipunctatus. Besides in well-structured species, elevated levels of genetic polymorphism were found inside T. turskyi and T. ukliva, as a consequence of their old origin and unconstrained evolutionary history.
[Mh] Termos MeSH primário: Evolução Biológica
Cipriniformes/genética
Rios
[Mh] Termos MeSH secundário: Animais
Cipriniformes/classificação
Citocromos b/genética
Europa (Continente)
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9035-37-4 (Cytochromes b)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187366


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[PMID]:28873616
[Au] Autor:Pérez M; Santafé-Muñoz AM; Balado M; Presa P
[Ad] Endereço:Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, 36200 Vigo, Spain. Electronic address: montse.perez@ieo.es.
[Ti] Título:Methodological evaluation of DNA-based molecular keys to identify categories of mislabelling in commercial products from genus Merluccius spp.
[So] Source:Food Chem;239:640-648, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The large variety of fish formats which are globally commercialized supports use of meta-evaluation studies to test discrimination power among molecular keys available for traceability of highly-degraded and/or chemically-modified DNA material. This paper shows that a combination of DNA identification methods validated for genus Merluccius allows 100% species assignment in hake products and offers higher diagnostic power (97% on products) than individual methods, i.e. Hake-ITS1-RFLP (89%) or Hake-Cytochrome b-RFLP (83%). A global 31% product mislabelling involved 15% of products affected by internal species substitution, as the main cause of mislabelling in ultrafrozen products, and another 16% affected by external species substitution with non-hake species, as the cause of mislabelling in processed and cooked hake-based products. The combination of both DNA keys minimizes the tool-associated rate of diagnostic failure, allowing decoupling of mislabelling categories and maximizing the quantitative adscription of products to species on any kind of hake-based products.
[Mh] Termos MeSH primário: Gadiformes
[Mh] Termos MeSH secundário: Animais
Citocromos b
DNA
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA); 9035-37-4 (Cytochromes b)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:28748739
[Au] Autor:Ahamad MNU; Ali ME; Hossain MAM; Asing A; Sultana S; Jahurul MHA
[Ad] Endereço:a Nanotechnology and Catalysis Research Center, Institute of Graduate Studies , University of Malaya , Kuala Lumpur , Malaysia.
[Ti] Título:Multiplex PCR assay discriminates rabbit, rat and squirrel meat in food chain.
[So] Source:Food Addit Contam Part A Chem Anal Control Expo Risk Assess;34(12):2043-2057, 2017 Dec.
[Is] ISSN:1944-0057
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Rabbit meat is receiving increasing attention because it contains a high level of proteins with relatively little fat. On the other hand, squirrel meat is served in upper-class meals in certain countries, so is sold at higher prices. The other side of the coin is rat meat, which has family ties with rabbit and squirrel but poses substantial threats to public health because it is a potential carrier of several zoonotic organisms. Recently, rat meat was mislabelled and sold as lamb after chemical modification. Thus, the chances of rabbit and squirrel meat substitution by rat meat cannot be ruled out. For the first time, a multiplex PCR assay was developed in Malaysia for the discriminatory identification of rat, rabbit and squirrel in the food chain. Rabbit (123 bp), rat (108 bp) and squirrel (243 bp) targets were amplified from ATP6 and cytb genes, along with a eukaryotic internal control (141bp). The products were sequenced and cross-tested against 22 species. A total of 81 reference samples and 72 meatball specimens were screened to validate the assay. Analyte stability was evaluated through boiling, autoclaving and micro-oven cooking. The tested lower limits of detection were 0.01 ng DNA for pure meat and 0.1% for meatballs.
[Mh] Termos MeSH primário: Contaminação de Alimentos/análise
Abastecimento de Alimentos
Carne/análise
Reação em Cadeia da Polimerase Multiplex
Coelhos/genética
Ratos/genética
Sciuridae/genética
[Mh] Termos MeSH secundário: Animais
Citocromos b/genética
Malásia
ATPases Mitocondriais Próton-Translocadoras/genética
ATPases Mitocondriais Próton-Translocadoras/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9035-37-4 (Cytochromes b); EC 3.6.3.- (Mitochondrial Proton-Translocating ATPases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1080/19440049.2017.1359752


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[PMID]:28934263
[Au] Autor:Benabid M; Ghrab J; Rhim A; Ben-Romdhane R; Aoun K; Bouratbine A
[Ad] Endereço:Department of Parasitology, Research Lab: LR 11-IPT-06, Pasteur Institute of Tunis, University Tunis El-Manar, Tunis, Tunisia.
[Ti] Título:Temporal dynamics and Leishmania infantum infection prevalence of Phlebotomus perniciosus (Diptera, Phlebotominae) in highly endemic areas of visceral leishmaniasis in Tunisia.
[So] Source:PLoS One;12(9):e0184700, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phlebotomus perniciosus is one of the major vectors of Leishmania infantum in the Mediterranean basin. The aim of this work was (i) to provide information about abundance and temporal dynamics of this Larroussius species in a hot spot area of visceral leishmaniasis in Tunisia, (ii) to detect L. infantum DNA in wild caught female sandflies and (iii) to measure Phlebotomus perniciosus infection rate throughout the active season. Sandflies were collected monthly during one year using CDC miniature light-traps in house and in animal shelters. Male specimens were identified at species level according to morphological characters. Female specimens were conserved individually for molecular study. Leishmania infection was tested by kinetoplast DNA real-time PCR and ITS-1 PCR-sequencing. Subsequent sandfly species identification of infected specimens was done by mitochondrial cytochrome b sequencing. In one year period, overall 4,441 specimens (2230 males and 2211 females) were collected. Sandfly activity started in end-April and ended in early-November. Mean sandfly density in house was significantly lower than in animal shelters (51 ± 50 versus 504 ± 460 sandflies /CDC night, p<0.05). However, a higher proportion of females was found in house (58.4% versus 49.2%, p<0.001). Based on species identification of male specimens, Phlebotomus perniciosus was the dominant species (56% of the whole male sandfly fauna, p<0.0001). It showed two peaks of density in the active season, a sharp one in early May and a higher long lasting one from end-July to end-September. DNA was extracted from 190 female specimens randomly sampled and corresponding to 96 specimens from house and 94 from animal shelters. Twenty four female sandfly were infected by Leishmania infantum. All infected specimens were recognized as Phlebotomus perniciosus. Leishmania infantum infection rate in female sandflies was 2.3 fold higher in house than in animal shelters (17.7% versus 7.4%, p<0.05). In house, estimated number of infected specimens was the highest at the end of the active season. Abundance, dynamics of density and Leishmania infantum infection prevalence of Phlebotomus perniciosus in Tunisian hot spot of visceral leishmaniasis highlight the major role of this Phlebotominae species in L. infantum transmission.
[Mh] Termos MeSH primário: Leishmania infantum
Leishmaniose Visceral/epidemiologia
Phlebotomus/parasitologia
[Mh] Termos MeSH secundário: Animais
Citocromos b/genética
DNA de Protozoário
Doenças Endêmicas
Feminino
Habitação
Abrigo para Animais
Leishmania infantum/genética
Leishmaniose Visceral/transmissão
Masculino
Estações do Ano
Fatores de Tempo
Tunísia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan); 9035-37-4 (Cytochromes b)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184700



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