Base de dados : MEDLINE
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[PMID]:28394473
[Au] Autor:Chishiki M; Takagi K; Sato A; Miki Y; Yamamoto Y; Ebata A; Shibahara Y; Watanabe M; Ishida T; Sasano H; Suzuki T
[Ad] Endereço:Department of Pathology and Histotechnology, Tohoku University Graduate School of Medicine, Sendai, Japan.
[Ti] Título:Cytochrome c1 in ductal carcinoma in situ of breast associated with proliferation and comedo necrosis.
[So] Source:Cancer Sci;108(7):1510-1519, 2017 Jul.
[Is] ISSN:1349-7006
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:It is well known that comedo necrosis is closely associated with an aggressive phenotype of ductal carcinoma in situ (DCIS) of human breast, but its molecular mechanisms remain largely unclear. Therefore, in this study, we first examined the gene expression profile of comedo DCIS based on microarray data and identified CYC1 as a gene associated with comedo necrosis. Cytochrome c1 (CYC1) is a subunit of complex III in the mitochondrial oxidative phosphorylation that is involved in energy production. However, the significance of CYC1 has not yet been examined in DCIS. We therefore immunolocalized CYC1 in 47 DCIS cases. CYC1 immunoreactivity was detected in 40% of DCIS cases, and the immunohistochemical CYC1 status was significantly associated with tumor size, nuclear grade, comedo necrosis, van Nuys classification, and Ki-67 labeling index. Subsequent in vitro studies indicated that CYC1 was significantly associated with mitochondrial membrane potential in MCF10DCIS.com DCIS cells. Moreover, CYC1 significantly promoted proliferation activity of MCF10DCIS.com cells and the cells transfected with CYC1 siRNA decreased pro-apoptotic caspase 3 activity under hypoxic or anoxic conditions. Considering that the center of DCIS is poorly oxygenated, these results indicate that CYC1 plays important roles in cell proliferation and comedo necrosis through the elevated oxidative phosphorylation activity in human DCIS.
[Mh] Termos MeSH primário: Neoplasias da Mama/patologia
Carcinoma Intraductal não Infiltrante/patologia
Citocromos c1/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Neoplasias da Mama/metabolismo
Carcinoma Intraductal não Infiltrante/metabolismo
Feminino
Seres Humanos
Immunoblotting
Imuno-Histoquímica
Microdissecção e Captura a Laser
Meia-Idade
Necrose
Análise de Sequência com Séries de Oligonucleotídeos
Reação em Cadeia da Polimerase em Tempo Real
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9035-42-1 (Cytochromes c1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170411
[St] Status:MEDLINE
[do] DOI:10.1111/cas.13251


  2 / 470 MEDLINE  
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[PMID]:28391262
[Au] Autor:Fan L; Zhu C; Qiu R; Zan P; Zheng Z; Xu T; Li G
[Ad] Endereço:Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
[Ti] Título:MicroRNA-661 Enhances TRAIL or STS Induced Osteosarcoma Cell Apoptosis by Modulating the Expression of Cytochrome c1.
[So] Source:Cell Physiol Biochem;41(5):1935-1946, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:AIM: Osteosarcoma (OS) is an aggressive bone malignancy that affects rapidly growing bones and is associated with a poor prognosis. Our previous study showed that cytochrome c1 (CYC1), a subunit of the cytochrome bc1 complex (complex III) of the mitochondrial electron chain, is overexpressed in human OS tissues and cell lines and its silencing induces apoptosis in vitro and inhibits tumor growth in vivo. Here, we investigated the mechanism underlying the modulation of CYC1 expression in OS and its role in the resistance of OS to apoptosis. METHODS: qRT-PCR, luciferase reporter assay, western blotting, fow cytometry, and animal experiments were performed in this study. RESULTS: MicroRNA (miR)-661 was identified as a downregulated miRNA in OS tissues and cells and shown to directly target CYC1. Ectopically expressed miR-661 inhibited OS cell growth, promoted apoptosis, and reduced the activity of mitochondrial complex III. miR-661 overexpression enhanced TRAIL or STS induced apoptosis and promoted the release of cytochrome c into the cytosol, which induced caspase-9 activation, and these effects were abolished by a caspase-3 inhibitor. Overexpression of CYC1 rescued the effects of miR-661 on sensitizing OS cells to TRAIL or STS induced apoptosis, indicating that the antitumor effect of miR-661 is mediated by the downregulation of CYC1. In vivo, miR-661 overexpression sensitized tumors to TRAIL or STS induced apoptosis in a xenograft mouse model, and these effects were attenuated by co-expression of CYC1. CONCLUSION: Taken together, our results indicate that miR-661 plays a tumor suppressor role in OS mediated by the downregulation of CYC1, suggesting a potential mechanism underlying cell death resistance in OS.
[Mh] Termos MeSH primário: Apoptose
Neoplasias Ósseas/metabolismo
Citocromos c1/biossíntese
Regulação Enzimológica da Expressão Gênica
Regulação Neoplásica da Expressão Gênica
Genes Supressores de Tumor
MicroRNAs/biossíntese
Proteínas de Neoplasias/metabolismo
Osteossarcoma/metabolismo
RNA Neoplásico/biossíntese
Esteril-Sulfatase/metabolismo
Ligante Indutor de Apoptose Relacionado a TNF/metabolismo
[Mh] Termos MeSH secundário: Animais
Neoplasias Ósseas/genética
Neoplasias Ósseas/patologia
Citocromos c1/genética
Feminino
Seres Humanos
Masculino
Camundongos
Camundongos Nus
MicroRNAs/genética
Proteínas de Neoplasias/genética
Osteossarcoma/genética
Osteossarcoma/patologia
RNA Neoplásico/genética
Esteril-Sulfatase/genética
Ligante Indutor de Apoptose Relacionado a TNF/genética
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MIRN661 microRNA, human); 0 (MicroRNAs); 0 (Neoplasm Proteins); 0 (RNA, Neoplasm); 0 (TNF-Related Apoptosis-Inducing Ligand); 0 (TNFSF10 protein, human); 9035-42-1 (Cytochromes c1); EC 3.1.6.2 (STS protein, human); EC 3.1.6.2 (Steryl-Sulfatase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170410
[St] Status:MEDLINE
[do] DOI:10.1159/000472380


  3 / 470 MEDLINE  
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[PMID]:28388834
[Au] Autor:Haase D; Hermann B; Einsle O; Simon J
[Ad] Endereço:Microbial Energy Conversion and Biotechnology, Department of Biology, Technische Universität Darmstadt, Schnittspahnstraße 10, 64287, Darmstadt, Germany.
[Ti] Título:Epsilonproteobacterial hydroxylamine oxidoreductase (εHao): characterization of a 'missing link' in the multihaem cytochrome c family.
[So] Source:Mol Microbiol;105(1):127-138, 2017 07.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Members of the multihaem cytochrome c family such as pentahaem cytochrome c nitrite reductase (NrfA) or octahaem hydroxylamine oxidoreductase (Hao) are involved in various microbial respiratory electron transport chains. Some members of the Hao subfamily, here called εHao proteins, have been predicted from the genomes of nitrate/nitrite-ammonifying bacteria that usually lack NrfA. Here, εHao proteins from the host-associated Epsilonproteobacteria Campylobacter fetus and Campylobacter curvus and the deep-sea hydrothermal vent bacteria Caminibacter mediatlanticus and Nautilia profundicola were purified as εHao-maltose binding protein fusions produced in Wolinella succinogenes. All four proteins were able to catalyze reduction of nitrite (yielding ammonium) and hydroxylamine whereas hydroxylamine oxidation was negligible. The introduction of a tyrosine residue at a position known to cause covalent trimerization of Hao proteins did neither stimulate hydroxylamine oxidation nor generate the Hao-typical absorbance maximum at 460 nm. In most cases, the εHao-encoding gene haoA was situated downstream of haoC, which predicts a tetrahaem cytochrome c of the NapC/NrfH family. This suggested the formation of a membrane-bound HaoCA assembly reminiscent of the menaquinol-oxidizing NrfHA complex. The results indicate that εHao proteins form a subfamily of ammonifying cytochrome c nitrite reductases that represents a 'missing link' in the evolution of NrfA and Hao enzymes.
[Mh] Termos MeSH primário: Citocromos c/metabolismo
Oxirredutases/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Grupo dos Citocromos c
Citocromos a1/metabolismo
Citocromos c1/metabolismo
Epsilonproteobacteria/genética
Epsilonproteobacteria/metabolismo
Nitrato Redutases/metabolismo
Nitritos/metabolismo
Oxirredução
Oxirredutases/genética
Wolinella/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Cytochrome c Group); 0 (Nitrites); 9007-43-6 (Cytochromes c); 9035-35-2 (Cytochromes a1); 9035-42-1 (Cytochromes c1); 9048-78-6 (cytochrome C-552); EC 1.- (Oxidoreductases); EC 1.7.- (Nitrate Reductases); EC 1.7.2.6 (hydroxylamine dehydrogenase); EC 1.9.6.1 (nitrate reductase (cytochrome))
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.13690


  4 / 470 MEDLINE  
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[PMID]:28104086
[Au] Autor:Li J; Wang Y; Jin H; Li W; Yan C; Yan P; Zhang X; He S; Song Z
[Ad] Endereço:Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610065, PR China.
[Ti] Título:Identification of Triplophysa species from the Qinghai-Tibetan Plateau (QTP) and its adjacent regions through DNA barcodes.
[So] Source:Gene;605:12-19, 2017 Mar 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The genus Triplophysa is the largest and most difficult to identity morphologically fish group of superfamily Cobitoidea with 140 currently valid species, and is mainly distributed in the Qinghai-Tibetan Plateau (QTP) and adjacent regions. Most species within this genus possess highly similar morphological characteristics for adaption to the highland environment and are very difficult to be identified only based on morphology. The traditional species identification, mainly based on external morphological diagnostic characters, leads to inconsistent results in many cases. Herein, we provided a molecular method based on mitochondrial cytochrome c subunit I (COI) for the identification of Triplophysa fishes. Thirty-three Triplophysa species, 244 individuals, were used to determine whether barcoding was effective in discriminating species for this genus. The mean intraspecific and interspecific K2P distances ranged from 0 to 14.9% (mean, 2.9%) and 0 to 23.4% (mean, 9.7%), respectively. The tree-based analysis displayed most of species formed discrete clusters with strong bootstrap support values (>90%). The results showed that most of Triplophysa species could be identified by DNA barcode and indicated DNA barcode could be used as a molecular marker for these species.
[Mh] Termos MeSH primário: Cipriniformes/genética
Citocromos c1/genética
DNA/genética
Proteínas de Peixes/genética
Filogenia
[Mh] Termos MeSH secundário: Animais
Artefatos
Cipriniformes/classificação
Código de Barras de DNA Taxonômico/métodos
Expressão Gênica
Variação Genética
Filogeografia
Tibet
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 9007-49-2 (DNA); 9035-42-1 (Cytochromes c1)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE


  5 / 470 MEDLINE  
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[PMID]:27586496
[Au] Autor:Parey K; Fielding AJ; Sörgel M; Rachel R; Huber H; Ziegler C; Rajendran C
[Ad] Endereço:Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany. kristian.parey@biophys.mpg.de.
[Ti] Título:In meso crystal structure of a novel membrane-associated octaheme cytochrome c from the Crenarchaeon Ignicoccus hospitalis.
[So] Source:FEBS J;283(20):3807-3820, 2016 Oct.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Crenarchaeon Ignicoccus hospitalis lives in symbiosis with Nanoarchaeum equitans providing essential cell components and nutrients to its symbiont. Ignicoccus hospitalis shows an intriguing morphology that points toward an evolutionary role in driving compartmentalization. Therefore, the bioenergetics of this archaeal host-symbiont system remains a pressing question. To date, the only electron acceptor described for I. hospitalis is elemental sulfur, but the organism comprises genes that encode for enzymes involved in nitrogen metabolism, e.g., one nitrate reductase and two octaheme cytochrome c, Igni_0955 (IhOCC) and Igni_1359. Herein, we detail functional and structural studies of the highly abundant IhOCC, including an X-ray crystal structure at 1.7 Å resolution, the first three-dimensional structure of an archaeal OCC. The trimeric IhOCC is membrane associated and exhibits significant structural and functional differences to previously characterized homologs within the hydroxylamine oxidoreductases (HAOs) and octaheme cytochrome c nitrite reductases (ONRs). The positions and spatial arrangement of the eight hemes are highly conserved, but the axial ligands of the individual hemes 3, 6 and 7 and the protein environment of the active site show significant differences. Most notably, the active site heme 4 lacks porphyrin-tyrosine cross-links present in the HAO family. We show that IhOCC efficiently reduces nitrite and hydroxylamine, with possible relevance to detoxification or energy conservation. DATABASE: Structural data are available in the Protein Data Bank under the accession number 4QO5.
[Mh] Termos MeSH primário: Proteínas Arqueais/química
Citocromos c/química
Desulfurococcaceae/química
[Mh] Termos MeSH secundário: Proteínas Arqueais/genética
Proteínas Arqueais/metabolismo
Sítios de Ligação
Sequência Conservada
Cristalografia por Raios X
Citocromos a1/química
Citocromos a1/genética
Citocromos a1/metabolismo
Citocromos c/genética
Citocromos c/metabolismo
Citocromos c1/química
Citocromos c1/genética
Citocromos c1/metabolismo
Desulfurococcaceae/genética
Desulfurococcaceae/metabolismo
Evolução Molecular
Genes Arqueais
Heme/química
Modelos Moleculares
Nitrato Redutases/química
Nitrato Redutases/genética
Nitrato Redutases/metabolismo
Estrutura Quaternária de Proteína
Subunidades Proteicas
Eletricidade Estática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (Protein Subunits); 42VZT0U6YR (Heme); 9007-43-6 (Cytochromes c); 9035-35-2 (Cytochromes a1); 9035-42-1 (Cytochromes c1); EC 1.7.- (Nitrate Reductases); EC 1.9.6.1 (nitrate reductase (cytochrome))
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160903
[St] Status:MEDLINE
[do] DOI:10.1111/febs.13870


  6 / 470 MEDLINE  
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[PMID]:27520480
[Au] Autor:Posayapisit N; Songsungthong W; Koonyosying P; Falade MO; Uthaipibull C; Yuthavong Y; Shaw PJ; Kamchonwongpaisan S
[Ad] Endereço:National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Thanon Phahonyothin, Tambon Khlong Neung, Amphoe Khlong Luang, Pathum Thani 12120, Thailand.
[Ti] Título:Cytochrome c and c1 heme lyases are essential in Plasmodium berghei.
[So] Source:Mol Biochem Parasitol;210(1-2):32-36, 2016 Nov - Dec.
[Is] ISSN:1872-9428
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Malaria parasites possess a de novo heme synthetic pathway. Interestingly, this pathway is dispensable during the blood stages of development in mammalian hosts. The assembly of the two most important hemeproteins, cytochromes c and c1, is mediated by cytochrome heme lyase enzymes. Plasmodium spp. possess two cytochrome heme lyases encoded by separate genes. Given the redundancy of heme synthesis, we sought to determine if heme lyase function also exhibits redundancy. To answer this question, we performed gene knockout experiments. We found that the PBANKA_143950 and PBANKA_0602600 Plasmodium berghei genes encoding cytochrome c (Pbcchl) and cytochrome c1 (Pbcc hl) heme lyases, respectively, can only be disrupted when a complementary gene is present. In contrast, four genes in the de novo heme synthesis pathway can be disrupted without complementation. This work provides evidence that Pbcchl and Pbcc hl are both essential and thus may be antimalarial targets.
[Mh] Termos MeSH primário: Citocromos c1/metabolismo
Citocromos c/metabolismo
Heme/metabolismo
Plasmodium berghei/fisiologia
[Mh] Termos MeSH secundário: Citocromos c/genética
Citocromos c1/genética
Expressão Gênica
Regulação da Expressão Gênica
Marcação de Genes
Genes Essenciais
Vetores Genéticos/genética
Mitocôndrias/genética
Mitocôndrias/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
42VZT0U6YR (Heme); 9007-43-6 (Cytochromes c); 9035-42-1 (Cytochromes c1)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160814
[St] Status:MEDLINE


  7 / 470 MEDLINE  
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[PMID]:27239088
[Au] Autor:Han Y; Sun S; Zhao M; Zhang Z; Gong S; Gao P; Liu J; Zhou J; Ma D; Gao Q; Wu P
[Ad] Endereço:Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
[Ti] Título:CYC1 Predicts Poor Prognosis in Patients with Breast Cancer.
[So] Source:Dis Markers;2016:3528064, 2016.
[Is] ISSN:1875-8630
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cytochrome c-1 (CYC1) is an important subunit of mitochondrial complex III. However, its role in tumor progression is unclear. We found that CYC1 was upregulated in breast tumor tissues, especially in tissues with lymph node metastasis. And higher expression of CYC1 correlates with poor prognosis in breast cancer patients using online databases and tools. Then we confirmed that CYC1 contributed to metastasis and proliferation in two highly metastatic human breast cancer cell lines. Digging into the biological function of CYC1, we found the activity of mitochondrial complex III decreased due to silencing CYC1. Then the ratio of AMP to ATP increased and AMPK was activated. Analyzing units of other mitochondrial complexes, we did not find knockdown of CYC1 expression reduced expression of any other unit of OXPHOS. We concluded that CYC1 promoted tumor metastasis via suppressing activation of AMPK and contributed to tumor growth via facilitating production of ATP. Our results indicated that CYC1 plays crucial roles in breast cancer progression and might be a predictive factor assisting future patient diagnosis.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo
Neoplasias da Mama/metabolismo
Carcinoma Ductal/metabolismo
Citocromos c1/metabolismo
[Mh] Termos MeSH secundário: Proteínas Quinases Ativadas por AMP/metabolismo
Trifosfato de Adenosina/metabolismo
Adulto
Biomarcadores Tumorais/genética
Neoplasias da Mama/patologia
Carcinoma Ductal/patologia
Linhagem Celular Tumoral
Proliferação Celular
Citocromos c1/genética
Feminino
Seres Humanos
Meia-Idade
Metástase Neoplásica
Fosforilação Oxidativa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 8L70Q75FXE (Adenosine Triphosphate); 9035-42-1 (Cytochromes c1); EC 2.7.11.31 (AMP-Activated Protein Kinases)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160531
[St] Status:MEDLINE
[do] DOI:10.1155/2016/3528064


  8 / 470 MEDLINE  
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[PMID]:27062510
[Au] Autor:Sonntag KC; Tejada G; Subburaju S; Berretta S; Benes FM; Woo TU
[Ad] Endereço:Division of Basic Neuroscience, Department of Psychiatry, Harvard Brain Tissue Resource Center, McLean Hospital/Harvard Medical School, Belmont, Massachusetts, USA.
[Ti] Título:Limited predictability of postmortem human brain tissue quality by RNA integrity numbers.
[So] Source:J Neurochem;138(1):53-9, 2016 Jul.
[Is] ISSN:1471-4159
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The RNA integrity number (RIN) is often considered to be a critical measure of the quality of postmortem human brains. However, it has been suggested that RINs do not necessarily reflect the availability of intact mRNA. Using the Agilent bioanalyzer and qRT-PCR, we explored whether RINs provide a meaningful way of assessing mRNA degradation and integrity in human brain samples by evaluating the expression of 3'-5' mRNA sequences of the cytochrome C-1 (CYC1) gene. Analysis of electropherograms showed that RINs were not consistently correlated with RNA or cDNA profiles and appeared to be poor predictors of overall cDNA quality. Cycle thresholds from qRT-PCR analysis to quantify the amount of CYC1 mRNA revealed positive correlations of RINs with amplification of full-length transcripts, despite the variable degree of linear degradation along the 3'-5' sequence. These data demonstrate that in postmortem human brain tissue the RIN is an indicator of mRNA quantity independent of degradation, but does not predict mRNA integrity, suggesting that RINs provide an incomplete measure of brain tissue quality. Quality assessment of postmortem human brains by RNA integrity numbers (RINs) may be misleading, as they do not measure intact mRNAs. We show that the RIN is an indicator of mRNA quantity independent of degradation, but does not predict mRNA integrity, suggesting that RINs provide an incomplete measure of brain tissue quality. Our results resolve controversial assumption on interpreting quality assessments of human postmortem brains by RINs.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Encéfalo/patologia
Citocromos c1/genética
RNA/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Células Cultivadas
Fibroblastos
Perfilação da Expressão Gênica
Seres Humanos
Transtornos Mentais/patologia
Meia-Idade
Doenças Neurodegenerativas/patologia
Mudanças Depois da Morte
Valor Preditivo dos Testes
Estabilidade de RNA/fisiologia
RNA Mensageiro/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 63231-63-0 (RNA); 9035-42-1 (Cytochromes c1)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160411
[St] Status:MEDLINE
[do] DOI:10.1111/jnc.13637


  9 / 470 MEDLINE  
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[PMID]:26395430
[Au] Autor:Kern M; Simon J
[Ad] Endereço:Microbial Energy Conversion and Biotechnology, Department of Biology, Technische Universität Darmstadt, Schnittspahnstraße 10, 64287, Darmstadt, Germany.
[Ti] Título:Three transcription regulators of the Nss family mediate the adaptive response induced by nitrate, nitric oxide or nitrous oxide in Wolinella succinogenes.
[So] Source:Environ Microbiol;18(9):2899-912, 2016 Sep.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sensing potential nitrogen-containing respiratory substrates such as nitrate, nitrite, hydroxylamine, nitric oxide (NO) or nitrous oxide (N2 O) in the environment and subsequent upregulation of corresponding catabolic enzymes is essential for many microbial cells. The molecular mechanisms of such adaptive responses are, however, highly diverse in different species. Here, induction of periplasmic nitrate reductase (Nap), cytochrome c nitrite reductase (Nrf) and cytochrome c N2 O reductase (cNos) was investigated in cells of the Epsilonproteobacterium Wolinella succinogenes grown either by fumarate, nitrate or N2 O respiration. Furthermore, fumarate respiration in the presence of various nitrogen compounds or NO-releasing chemicals was examined. Upregulation of each of the Nap, Nrf and cNos enzyme systems was found in response to the presence of nitrate, NO-releasers or N2 O, and the cells were shown to employ three transcription regulators of the Crp-Fnr superfamily (homologues of Campylobacter jejuni NssR), designated NssA, NssB and NssC, to mediate the upregulation of Nap, Nrf and cNos. Analysis of single nss mutants revealed that NssA controls production of the Nap and Nrf systems in fumarate-grown cells, while NssB was required to induce the Nap, Nrf and cNos systems specifically in response to NO-generators. NssC was indispensable for cNos production under any tested condition. The data indicate dedicated signal transduction routes responsive to nitrate, NO and N2 O and imply the presence of an N2 O-sensing mechanism.
[Mh] Termos MeSH primário: Nitrato Redutase/genética
Nitratos/metabolismo
Óxido Nítrico/metabolismo
Óxido Nitroso/metabolismo
Fatores de Transcrição/metabolismo
Wolinella/genética
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Citocromos a1/biossíntese
Citocromos a1/genética
Citocromos c1/biossíntese
Citocromos c1/genética
Regulação Bacteriana da Expressão Gênica
Nitrato Redutase/biossíntese
Nitrato Redutase/metabolismo
Nitrato Redutases/biossíntese
Nitrato Redutases/genética
Fatores de Transcrição/genética
Regulação para Cima
Wolinella/enzimologia
Wolinella/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrates); 0 (Transcription Factors); 31C4KY9ESH (Nitric Oxide); 9035-35-2 (Cytochromes a1); 9035-42-1 (Cytochromes c1); EC 1.7.- (Nitrate Reductases); EC 1.7.99.4 (Nitrate Reductase); EC 1.9.6.1 (nitrate reductase (cytochrome)); K50XQU1029 (Nitrous Oxide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150924
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13060


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[PMID]:26809915
[Au] Autor:Esmaelizad M; Zeinedin H; Razmaraii N; Mirjalili A
[Ad] Endereço:Department of Central Laboratory, Razi Vaccine and Serum Research Institute, Karaj, Iran. m.esmaelizad@rvsri.ac.ir.
[Ti] Título:Molecular Study of the G1 Haplotypes of Echinococcus granulosus from Iran Based on Cytochrome C Oxidase (Subunit 1) Sequence.
[So] Source:Turkiye Parazitol Derg;39(4):286-90, 2015 Dec.
[Is] ISSN:2146-3077
[Cp] País de publicação:Turkey
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: In this study, we attempted to identify new Echinococcus granulosus isolates in the North West provinces of Iran based on the mitochondrial cytochrome c oxidase subunit 1 (CO1) sequence. METHODS: Twenty-nine hydatid cysts from sheep and goats were collected. Genomic DNAs were extracted, and a partial sequence of the CO1 gene was amplified. Polymerase chain reaction products were cloned and sequenced with M13 primers in both directions. RESULTS: All Iranian isolates were located in G1 and G3 genotypes. For the first time, a new G1 haplotype in two Iranian isolates were identified. CONCLUSION: It seems that this new haplotype was transmitted from Jordan to Iran or vice versa.
[Mh] Termos MeSH primário: Citocromos c1/genética
Equinococose Hepática/veterinária
Echinococcus granulosus/classificação
Complexo IV da Cadeia de Transporte de Elétrons/genética
Doenças das Cabras/parasitologia
Doenças dos Ovinos/parasitologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Citocromos c1/química
DNA de Helmintos/química
DNA de Helmintos/isolamento & purificação
Equinococose Hepática/parasitologia
Echinococcus granulosus/genética
Echinococcus granulosus/isolamento & purificação
Genótipo
Técnicas de Genotipagem
Cabras
Haplótipos
Irã (Geográfico)
Mitocôndrias/enzimologia
Filogenia
Reação em Cadeia da Polimerase
Polimorfismo Genético
Alinhamento de Sequência
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Helminth); 9035-42-1 (Cytochromes c1); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161021
[Lr] Data última revisão:
161021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160127
[St] Status:MEDLINE
[do] DOI:10.5152/tpd.2015.4292



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