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[PMID]:22642120
[Au] Autor:Marchenko MM; Kopyl'chuk HP; Shmarakov IO; Buchkovs'ka IM
[Ti] Título:[Activity of enzymatic detoxification systems in the mice liver under conditions of different retinoid provision].
[So] Source:Ukr Biokhim Zh (1999);84(2):42-7, 2012 Mar-Apr.
[Cp] País de publicação:Ukraine
[La] Idioma:ukr
[Ab] Resumo:The activity of cellular components of liver detoxification system was studied under the conditions of the absence of vitamin A stores. It is shown that a decrease of p-hydroxylase and N-demethylase activity of cytochrome P-450 simultaneously with a decrease of glutathione-S-transferase activity takes place in the liver microsomal fraction of vitamin A-deficient animals. At the same time the absence of retinoid stores in knock-out animals influences the decrease of only p-hydroxylase activity of cytochrome P-450 system. The increase in glutathione-S-transferase activity is observed in the liver postmicrosomal fraction in mice, kept on vitamin A-deficient diet, while its parametres in knock-out group animals were not statistically different compared to the control.
[Mh] Termos MeSH primário: Compostos de Anilina/metabolismo
Anilina Hidroxilase/metabolismo
Glutationa Transferase/metabolismo
Fígado/enzimologia
Microssomos Hepáticos/enzimologia
Oxirredutases N-Desmetilantes/metabolismo
Deficiência de Vitamina A/metabolismo
[Mh] Termos MeSH secundário: Aciltransferases/deficiência
Aciltransferases/genética
Compostos de Anilina/toxicidade
Animais
Dieta
Glutationa/metabolismo
Inativação Metabólica
Fígado/efeitos dos fármacos
Camundongos
Camundongos Knockout
Microssomos Hepáticos/efeitos dos fármacos
Vitamina A/metabolismo
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aniline Compounds); 11103-57-4 (Vitamin A); EC 1.14.14.- (Aniline Hydroxylase); EC 1.5.- (Oxidoreductases, N-Demethylating); EC 2.3.- (Acyltransferases); EC 2.3.1.- (lecithin-retinol acyltransferase); EC 2.5.1.18 (Glutathione Transferase); GAN16C9B8O (Glutathione); SIR7XX2F1K (aniline)
[Em] Mês de entrada:1206
[Cu] Atualização por classe:150803
[Lr] Data última revisão:
150803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120531
[St] Status:MEDLINE


  2 / 729 MEDLINE  
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[PMID]:22392284
[Au] Autor:Sen A; Ozgun O; Arinç E; Arslan S
[Ad] Endereço:Department of Biology, Pamukkale University, 20070, Denizli, Turkey.
[Ti] Título:Diverse action of acrylamide on cytochrome P450 and glutathione S-transferase isozyme activities, mRNA levels and protein levels in human hepatocarcinoma cells.
[So] Source:Cell Biol Toxicol;28(3):175-86, 2012 Jun.
[Is] ISSN:1573-6822
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Humans are exposed to acrylamide in their diet and cigarette smoke. Acrylamide is metabolized into glycidamide by CYP2E1. However, very few studies regarding the effects of acrylamide on cytochrome P450 and Glutathione S-Transferase (GST) isozymes have been pursued. The aim of this study is to elucidate the effects of acrylamide on cytochrome P450 and GST isozymes in HepG2 cell line. Treatment with 1.25 and 2.5 mM acrylamide caused 9.5- and 3.7-fold increases and 4.0- and 3.3-fold increases in CYP1A-associated ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase (MROD) activities, respectively. These increases were consistent with increases in mRNA and protein levels of these isozymes. Similarly, CYP2E1-associated aniline 4-hydroxylase (ANH) activity, protein levels, and mRNA levels increased 2.1- and 2.6-fold, 2.4- and 3.2-fold, and 1.4- and 1.9-fold following 1.25 and 2.5 mM acrylamide treatments, respectively. In addition, GST-mu activity was increased 2.4- and 5.1-fold by acrylamide. Moreover, GST-mu mRNA and protein levels increased twofold as a result of acrylamide treatment. In contrast, GST-pi protein and mRNA levels decreased significantly. In conclusion, human cell exposure to acrylamide causes an increase in the levels of carcinogenicity and toxicity and a disturbance in drug metabolism, possibly due to complex effects on P450 and GST isozymes.
[Mh] Termos MeSH primário: Acrilamida/toxicidade
Citocromo P-450 CYP2E1/metabolismo
Regulação Enzimológica da Expressão Gênica
Glutationa Transferase/metabolismo
RNA Mensageiro/metabolismo
[Mh] Termos MeSH secundário: Anilina Hidroxilase/genética
Anilina Hidroxilase/metabolismo
Testes de Carcinogenicidade
Sobrevivência Celular
Citocromo P-450 CYP1A1/metabolismo
Citocromo P-450 CYP2E1/genética
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Ativação Enzimática/efeitos dos fármacos
Ensaios Enzimáticos
Regulação Neoplásica da Expressão Gênica
Glutationa Transferase/genética
Células Hep G2
Seres Humanos
Isoenzimas/efeitos dos fármacos
Isoenzimas/genética
Isoenzimas/metabolismo
Testes de Toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Isoenzymes); 0 (RNA, Messenger); 20R035KLCI (Acrylamide); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.- (methoxyresorufin-O-demethylase); EC 1.14.13.- (Cytochrome P-450 CYP2E1); EC 1.14.14.- (Aniline Hydroxylase); EC 1.14.14.1 (Cytochrome P-450 CYP1A1); EC 2.5.1.18 (Glutathione Transferase)
[Em] Mês de entrada:1208
[Cu] Atualização por classe:141120
[Lr] Data última revisão:
141120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120307
[St] Status:MEDLINE
[do] DOI:10.1007/s10565-012-9214-1


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[PMID]:22359349
[Au] Autor:Lukivskaya OY; Naruta E; Sadovnichy V; Kirko S; Buko VU
[Ad] Endereço:Division of Biochemical Pharmacology, Institute of Pharmacology and Biochemistry, National Academy of Sciences, Grodno, Belarus.
[Ti] Título:Reversal of experimental ethanol-induced liver steatosis by borage oil.
[So] Source:Phytother Res;26(11):1626-31, 2012 Nov.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of study was to evaluate the hepatoprotective effect of borage oil containing predominantly gamma-linolenic acid in rats with alcoholic steatohepatitis. Liver of ethanol-treated animals was characterized by fatty and hydropic dystrophies. Liver triglyceride contents and activitiies of serum marker enzymes were significantly increased. Ethanol increased nicotinamide adenine dinucleotide phosphate hydrogen (NADPH)-induced chemiluminescence and the contents of liver thiobarbituric acid reactive substances (TBARS). The reduced glutathione content in the liver was decreased. Ethanol enhanced liver microsomal cytochrome P-450 (CYP450) content, aniline p-hydroxylase and amydopyrine-N-demethylase activities. The treatment with borage oil improved the liver morphology, decreased triglyceride contents and normalized serum marker enzyme activities. Borage oil developed an antioxidant effect in ethanol-treated rats. The treatment with this compound decreased NADPH-induced chemiluminescence and the content of lipid peroxidation products. Borage oil normalized CYP450 content compared with the ethanol-treated group. CYPI450 2E1 isoform is a main source of free oxygen radicals in the liver of ethanol-treated rats and we propose that the antioxidant effect of borage oil is realized via the normalization of CYP450 content and activities of CYP450-related microsomal oxidases, as borage oil can improve the lipid surrounding of CYP450. In our opinion, the hepatoprotection by borage oil in alcoholic steatosis is connected with its antioxidant properties.
[Mh] Termos MeSH primário: Fígado Gorduroso Alcoólico/tratamento farmacológico
Fígado/efeitos dos fármacos
Óleos Vegetais/farmacologia
Ácido gama-Linolênico/farmacologia
[Mh] Termos MeSH secundário: Aminopirina N-Desmetilase/metabolismo
Anilina Hidroxilase/metabolismo
Animais
Antioxidantes/farmacologia
Sistema Enzimático do Citocromo P-450/metabolismo
Etanol
Peroxidação de Lipídeos/efeitos dos fármacos
Fígado/enzimologia
Masculino
Microssomos Hepáticos/enzimologia
NADP/análise
Ratos
Ratos Wistar
Substâncias Reativas com Ácido Tiobarbitúrico/análise
Triglicerídeos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Oils); 0 (Thiobarbituric Acid Reactive Substances); 0 (Triglycerides); 0 (borage oil); 3K9958V90M (Ethanol); 53-59-8 (NADP); 78YC2MAX4O (gamma-Linolenic Acid); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.14.- (Aniline Hydroxylase); EC 1.5.3.- (Aminopyrine N-Demethylase); EC 1.5.3.- (amidopyrine N-demethylase)
[Em] Mês de entrada:1301
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120224
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.4621


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[PMID]:21705300
[Au] Autor:Vitcheva V; Simeonova R; Karova D; Mitcheva M
[Ad] Endereço:Department of Pharmacology, Pharmacotherapy and Toxicology, Medical University Sofia, Bulgaria. vesselavitcheva@yahoo.com
[Ti] Título:Nifedipine lowers cocaine-induced brain and liver enzyme activity and cocaine urinary excretion in rats.
[So] Source:Arh Hig Rada Toksikol;62(2):131-7, 2011 Jun.
[Is] ISSN:1848-6312
[Cp] País de publicação:Croatia
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to see how nifedipine counters the effects of cocaine on hepatic and brain enzymatic activity in rats and whether it affects urinary excretion of cocaine. Male Wistar rats were divided in four groups of six: control, nifedipine group (5 mg kg-1i.p. a day for five days); cocaine group (15 mg kg-1i.p. a day for five days), and the nifedipine+cocaine group. Twenty-four hours after the last administration, we measured neuronal nitric oxide synthase (nNOS) activity in the brain and cytochrome P450 quantity, ethylmorphine-N-demethylase, and anilinehydroxylase activity in the liver. Urine samples were collected 24 h after the last cocaine and cocaine+nifedipine administration. Urinary cocaine concentration was determined using the GC/MS method.Cocaine administration increased brain nNOS activity by 55 % (p<0.05) in respect to control, which indicates the development of tolerance and dependence. In the combination group, nifedipine decreased the nNOS activity in respect to the cocaine-only group.In the liver, cocaine significantly decreased and nifedipine significantly increased cytochrome P450, ethylmorphine-N-demethylase, and anilinehydroxylase in respect to control. In combination, nifedipine successfully countered cocaine effects on these enzymes.Urine cocaine excretion in the cocaine+nifedipine group significantly dropped (by 35 %) compared to the cocaine-only group.Our results have confirmed the effects of nifedipine against cocaine tolerance and development of dependence, most likely due to metabolic interactions between them.
[Mh] Termos MeSH primário: Encéfalo/enzimologia
Cocaína/toxicidade
Fígado/enzimologia
Nifedipino/farmacologia
[Mh] Termos MeSH secundário: Anilina Hidroxilase/metabolismo
Animais
Cocaína/urina
Sistema Enzimático do Citocromo P-450/metabolismo
Etilmorfina-N-Demetilasa/metabolismo
Glutationa/metabolismo
Masculino
Óxido Nítrico Sintase Tipo I/metabolismo
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.13.39 (Nitric Oxide Synthase Type I); EC 1.14.14.- (Aniline Hydroxylase); EC 1.5.3.- (Ethylmorphine-N-Demethylase); GAN16C9B8O (Glutathione); I5Y540LHVR (Cocaine); I9ZF7L6G2L (Nifedipine)
[Em] Mês de entrada:1111
[Cu] Atualização por classe:161205
[Lr] Data última revisão:
161205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110628
[St] Status:MEDLINE
[do] DOI:10.2478/10004-1254-62-2011-2086


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[PMID]:21338242
[Au] Autor:Kumar SS; Karrunakaran CM; Rao MR; Balasubramanian MP
[Ad] Endereço:Department of Industrial Biotechnology, Bharath University, Chennai, India. selvakumarmss@gmail.com
[Ti] Título:Indigofera aspalathoides protection against 20-methylcholanthrene-induced experimental fibrosarcoma growth after transplantation in rats - role of xenobiotic drug metabolizing enzymes.
[So] Source:Asian Pac J Cancer Prev;11(6):1833-8, 2010.
[Is] ISSN:2476-762X
[Cp] País de publicação:Thailand
[La] Idioma:eng
[Ab] Resumo:A large number of active principles from traditional medicinal plants have been reported to have chemopreventive properties. In the present study, therapeutic efficacy of an aqueous extract of Indigofera aspalathoides against growth of transplanted experimental fibrosarcomas in Wistar strain male albino rats was tested. Tumors which appeared about six weeks after implantation were highly localized and were maintained by serial transplantation. Rats were divided into four groups. Group I served as normal control animals. Group II were fibrosarcoma bearing animals. Group III were animals with fibrosarcoma treated with Indigofera aspalathoides aqueous extracts at a dose of 250 mg/kg. b. w. per day for 30 days. Group IV animals were treated with aqueous extract of Indigofera aspalathoides alone. Reduction in tumor weight was noted in Group III as compared to II. The levels of cytochrome C in liver and kidney, the levels of cytochrome P450 and cytochrome b5 in liver microsomes, phase I biotransformation enzymes NADPH-cytochrome P450, NADPH-cytochrome b5, and aniline hydroxylase, and the phase II enzymes glutathione-S-transferase and UDP glucuronyl transferase indicated that their modulation played a role in the therapeutic efficacy of Indigofera aspalathoides against experimental fibrosarcoma.
[Mh] Termos MeSH primário: Inativação Metabólica
Indigofera/química
Fitoterapia
Extratos Vegetais/uso terapêutico
Sarcoma Experimental/prevenção & controle
Xenobióticos/metabolismo
[Mh] Termos MeSH secundário: Anilina Hidroxilase/metabolismo
Animais
Biotransformação
Sistema Enzimático do Citocromo P-450/metabolismo
Citocromos b5/metabolismo
Glucuronosiltransferase/metabolismo
Glutationa Transferase/metabolismo
Masculino
Metilcolantreno
Microssomos Hepáticos/efeitos dos fármacos
Microssomos Hepáticos/enzimologia
Ratos
Ratos Wistar
Sarcoma Experimental/induzido quimicamente
Sarcoma Experimental/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Xenobiotics); 56-49-5 (Methylcholanthrene); 9035-39-6 (Cytochromes b5); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.14.- (Aniline Hydroxylase); EC 2.4.1.17 (Glucuronosyltransferase); EC 2.5.1.18 (Glutathione Transferase); EC 4.4.1.20 (leukotriene-C4 synthase)
[Em] Mês de entrada:1107
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110223
[St] Status:MEDLINE


  6 / 729 MEDLINE  
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[PMID]:21240366
[Au] Autor:Tseilikman VE; Ryabinin VE; Popova AS; Krupitskaya LI; Sinitsky AI
[Ad] Endereço:Chelyabinsk State Medical Academy, Russian Ministry of Health, Chelyabinsk, Russia. vadimed@yandex.ru
[Ti] Título:Variability of microsomal oxidation and porphyrin metabolism in rats.
[So] Source:Bull Exp Biol Med;150(2):178-9, 2010 Dec.
[Is] ISSN:1573-8221
[Cp] País de publicação:United States
[La] Idioma:eng; rus
[Ab] Resumo:An inverse relationship between erythropoiesis intensity and microsomal oxidation level has been detected during the early postnatal period in rats with high resistance to hypoxia.
[Mh] Termos MeSH primário: Heme/biossíntese
Microssomos/metabolismo
Porfirinas/metabolismo
[Mh] Termos MeSH secundário: Anilina Hidroxilase/metabolismo
Animais
Animais Recém-Nascidos
Sistema Enzimático do Citocromo P-450/metabolismo
Hexobarbital/farmacologia
Oxirredução
Ratos
Sono/efeitos dos fármacos
Sono/fisiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Porphyrins); 42VZT0U6YR (Heme); 9035-51-2 (Cytochrome P-450 Enzyme System); AL8Z8K3P6S (Hexobarbital); EC 1.14.14.- (Aniline Hydroxylase)
[Em] Mês de entrada:1105
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110118
[St] Status:MEDLINE


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[PMID]:19645331
[Au] Autor:Ozen T; Korkmaz H
[Ad] Endereço:Department of Chemistry, Faculty of Arts and Sciences, Giresun University, TR-28049 Giresun, Turkey. ozentevfik@hotmail.com
[Ti] Título:The effects of Urtica dioica L. leaf extract on aniline 4-hydroxylase in mice.
[So] Source:Acta Pol Pharm;66(3):305-9, 2009 May-Jun.
[Is] ISSN:0001-6837
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:The effects of hydroalcoholic (80% ethanol-20% water) extract of Urtica dioica L. on microsomal aniline 4-hydroxylase (A4H) were investigated in the liver of Swiss albino mice (8- 10-weeks-old) treated with two doses (50 and 100 mg/kg body weight, given orally for 14 days ). The activities of A4H showed a significant increase in the liver at both dose levels of extract treatment. The hydroalcoholic extract of Urtica dioica induced the activities of A4H that had been increased by treatment of metal ions (Mg2+ and Ca2+) and the mixture of cofactors (NADH and NADPH). At saturated concentration of cofactor, microsomal A4H exhibited significantly even higher activities in the presence of the mixture of cofactors than NADPH and NADH. Mg2+ and Ca2+ ions acted as stimulants in vitro. The present results suggest that the hydroalcoholic extract of Urtica dioica may have modalatory effect on aniline hydroxylase at least in part and enhance the activity of A4H adding metals ions and cofactors.
[Mh] Termos MeSH primário: Anilina Hidroxilase/efeitos dos fármacos
Microssomos Hepáticos/efeitos dos fármacos
Extratos Vegetais/farmacologia
Urtica dioica/química
[Mh] Termos MeSH secundário: Administração Oral
Anilina Hidroxilase/metabolismo
Animais
Cálcio/farmacologia
Relação Dose-Resposta a Droga
Indução Enzimática/efeitos dos fármacos
Magnésio/farmacologia
Masculino
Camundongos
Microssomos Hepáticos/enzimologia
NAD/farmacologia
NADP/farmacologia
Extratos Vegetais/administração & dosagem
Folhas de Planta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0U46U6E8UK (NAD); 53-59-8 (NADP); EC 1.14.14.- (Aniline Hydroxylase); I38ZP9992A (Magnesium); SY7Q814VUP (Calcium)
[Em] Mês de entrada:0908
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090804
[St] Status:MEDLINE


  8 / 729 MEDLINE  
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[PMID]:18051907
[Au] Autor:Zhang H; Song J; Zhan XA; Tan Y
[Ad] Endereço:Department of Pharmacy, People's Hospital, Wuhan University, Wuhan 430060, China. zhzx8888@163.com
[Ti] Título:[Effects of ethyl acetate extract of Semen Hoveniae on liver microsomal cytochrome P450 isoenzyme in rat].
[So] Source:Zhongguo Zhong Yao Za Zhi;32(18):1917-21, 2007 Sep.
[Is] ISSN:1001-5302
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To investigate the effects of the ethyl acetate extract of Semen Hoveniae (ESH) on liver microsomal cytochrome P450 isoenzyme in rats. METHOD: The rats were given orally the ESH in the doses of 0.14, 0.17, 0.2 g x kg (equivalent to the crude herb) for 10 days respectively. Rat liver microsomal cytochrome P450, NADPH-Cyt C reductase, erythromycin N-demethylase (ERD), Aniline hydroxylase (ANH), aminopyrine N-demethylase (ADM) activities were quantitated by UV chromatography. The levels of mRNA expression of CYP1A1, CYP2C11, CYP2E1 and CYP3A1 were detected by semi-quantitative reverse transcripatase-polymerase chain reaction (RT-PCR). RESULT: The cytochrome P450 content, NADPH-Cyt C reductase activities and erythromycin N-demethylase (ERD) activities were not affected. Aniline hydroxylase (ANH) activities in liver were decreased by up to35.1%; aminopyrine N-demethylase (ADM) activitiesin liver were increased by up to 42.4%. The mRNA expression of CYP1A1, CYP2C11 and CYP3A1 were found to be increased markedly. CONCLUSION: A specific effect of ESH on liver microsomal cytochrome P450 isoenzyme in rats was observed in this investigation. ESH had various effects on liver microsomal cytochrome P450 isoenzyme.
[Mh] Termos MeSH primário: Sistema Enzimático do Citocromo P-450/metabolismo
Medicamentos de Ervas Chinesas/farmacologia
Microssomos Hepáticos/efeitos dos fármacos
Rhamnaceae/química
[Mh] Termos MeSH secundário: Acetatos/química
Aminopirina N-Desmetilase/metabolismo
Anilina Hidroxilase/genética
Anilina Hidroxilase/metabolismo
Animais
Hidrocarboneto de Aril Hidroxilases/genética
Hidrocarboneto de Aril Hidroxilases/metabolismo
Citocromo P-450 CYP1A1/genética
Citocromo P-450 CYP1A1/metabolismo
Citocromo P-450 CYP2E1/genética
Citocromo P-450 CYP2E1/metabolismo
Citocromo P-450 CYP3A/genética
Citocromo P-450 CYP3A/metabolismo
Sistema Enzimático do Citocromo P-450/genética
Família 2 do Citocromo P450
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/isolamento & purificação
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Masculino
Microssomos Hepáticos/enzimologia
NADPH-Ferri-Hemoproteína Redutase/genética
NADPH-Ferri-Hemoproteína Redutase/metabolismo
Plantas Medicinais/química
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Distribuição Aleatória
Ratos
Ratos Wistar
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Sementes/química
Esteroide 16-alfa-Hidroxilase/genética
Esteroide 16-alfa-Hidroxilase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acetates); 0 (Drugs, Chinese Herbal); 0 (RNA, Messenger); 76845O8NMZ (ethyl acetate); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.13.- (Cytochrome P-450 CYP2E1); EC 1.14.14.- (Aniline Hydroxylase); EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases); EC 1.14.14.1 (CYP2C11 protein, rat); EC 1.14.14.1 (Cyp3a1 protein, rat); EC 1.14.14.1 (Cytochrome P-450 CYP1A1); EC 1.14.14.1 (Cytochrome P-450 CYP3A); EC 1.14.14.1 (Cytochrome P450 Family 2); EC 1.14.14.1 (Steroid 16-alpha-Hydroxylase); EC 1.5.3.- (Aminopyrine N-Demethylase); EC 1.6.2.4 (NADPH-Ferrihemoprotein Reductase)
[Em] Mês de entrada:0811
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071207
[St] Status:MEDLINE


  9 / 729 MEDLINE  
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[PMID]:17410408
[Au] Autor:Bhadauria M; Nirala SK; Shukla S
[Ad] Endereço:School of Studies in Zoology, Jiwaji University, Gwalior, 474011 MP, India. monikabhadauria@rediffmail.com
[Ti] Título:Propolis protects CYP 2E1 enzymatic activity and oxidative stress induced by carbon tetrachloride.
[So] Source:Mol Cell Biochem;302(1-2):215-24, 2007 Aug.
[Is] ISSN:0300-8177
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Induction of CYP 2E1 by carbon tetrachloride (CCl(4)) is one of the central pathways by which CCl(4) generates oxidative stress in hepatocytes. Experimental liver injury was induced in rats by CCl(4) to determine toxicological actions on CYP 2E1 by microsomal drug metabolizing enzymes. In this report, ethanolic extract of propolis at a dose of 200 mg/kg (po) was used after 24 h of toxicant administration to validate its protective potential. Intraperitoneal injection of CCl(4) (1.5 ml/kg) induced hepatotoxicity after 24 h of its administration that was associated with elevated malonyldialdehyde (index of lipid peroxidation), lactate dehydrogenase and gamma-glutamyl transpeptidase release (index of a cytotoxic effect). Hepatic microsomal drug metabolizing enzymes of CYP 2E1 showed sharp depletion as assessed by estimating aniline hydroxylase and amidopyrine N-demethylase activity after CCl(4) exposure. Toxic effect of CCl(4) was evident on CYP 2E1 activity by increased hexobarbitone induced sleep time and bromosulphalein retention. Propolis extract showed significant improvement in the activity of both enzymes and suppressed toxicant induced increase in sleep time and bromosulphalein retention. Choleretic activity of liver did not show any sign of toxicity after propolis treatment at a dose of 200 mg/kg (id). Histopathological evaluation of the liver revealed that propolis reduced the incidence of liver lesions including hepatocyte swelling and lymphocytic infiltrations induced by CCl(4). Electron microscopic observations also showed improvement in ultrastructure of liver and substantiated recovery in biochemical parameters. Protective activity of propolis at 200 mg/kg dose was statistically compared with positive control silymarin (50 mg/kg, po), a known hepatoprotective drug seems to be better in preventing hepatic CYP 2E1 activity deviated by CCl(4). These results lead us to speculate that propolis may play hepatoprotective role via improved CYP 2E1 activity and reduced oxidative stress in living system.
[Mh] Termos MeSH primário: Tetracloreto de Carbono/toxicidade
Citocromo P-450 CYP2E1/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Própole/farmacologia
Substâncias Protetoras/farmacologia
[Mh] Termos MeSH secundário: Aminopirina N-Desmetilase/metabolismo
Anilina Hidroxilase/metabolismo
Animais
Colagogos e Coleréticos/farmacologia
Hexobarbital/farmacologia
L-Lactato Desidrogenase/sangue
Peroxidação de Lipídeos/efeitos dos fármacos
Fígado/enzimologia
Fígado/patologia
Fígado/ultraestrutura
Microssomos Hepáticos/efeitos dos fármacos
Microssomos Hepáticos/enzimologia
Ratos
Ratos Sprague-Dawley
Descanso
Sono/efeitos dos fármacos
gama-Glutamiltransferase/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cholagogues and Choleretics); 0 (Protective Agents); 9009-62-5 (Propolis); AL8Z8K3P6S (Hexobarbital); CL2T97X0V0 (Carbon Tetrachloride); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.14.13.- (Cytochrome P-450 CYP2E1); EC 1.14.14.- (Aniline Hydroxylase); EC 1.5.3.- (Aminopyrine N-Demethylase); EC 1.5.3.- (amidopyrine N-demethylase); EC 2.3.2.2 (gamma-Glutamyltransferase)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070406
[St] Status:MEDLINE


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[PMID]:17034923
[Au] Autor:Arinç E; Arslan S; Bozcaarmutlu A; Adali O
[Ad] Endereço:Biochemistry Graduate Programme and Department of Biological Sciences, Middle East Technical University, Inonu Bulvari, 06531 Ankara, Turkey. earinc@metu.edu.tr
[Ti] Título:Effects of diabetes on rabbit kidney and lung CYP2E1 and CYP2B4 expression and drug metabolism and potentiation of carcinogenic activity of N-nitrosodimethylamine in kidney and lung.
[So] Source:Food Chem Toxicol;45(1):107-18, 2007 Jan.
[Is] ISSN:0278-6915
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:There are limited number of studies regarding the influence of diabetes on the regulation of cytochrome P450s and associated drug metabolizing enzyme activities especially in extrahepatic tissues such as kidney. However, there is almost no such study in lung. Alloxan-induced diabetes did not change CYP2B4 expression as measured with immunoblot analysis and associated enzyme, benzphetamine N-demethylase, activity in rabbit kidney and lung. Induction of cytochrome P4502E1 by diabetes was identified by immunochemical detection on Western blots in the lung and kidney microsomes of rabbits. In parallel to CYP2E1 induction, aniline 4-hydroxylase and p-nitrophenol hydroxylase activities were markedly increased in diabetic rabbit lung and kidney. CYP2B4 and CYP2E1 dependent drug metabolism did not show any tissue variation in diabetic rabbit. These findings are in contrast to those of rats, mice and hamster. The results of the present work, in combination with those of the previous work [Arinç, E., Arslan, S., Adali, O., 2005. Differential effects of diabetes on CYP2E1 and CYP2B4 proteins and associated drug metabolizing enzyme activities in rabbit liver. Arch. Toxicol. 79, 427-433], indicate the existence of species-dependent response of CYP-dependent drug metabolizing enzymes to diabetes. A procarcinogen and food contaminant, N-nitrosodimethylamine (NDMA), is converted to its carcinogenic form after it is activated with NDMA N-demethylase. In the current study, a statistically significant increase of liver, kidney and lung NDMA N-demethylase activity associated with CYP2E1 was shown in diabetic rabbit. Thus, it is expected that, the risk of nitrosamine induced carcinogenesis will be greater in liver, kidney and lung of the diabetic subjects.
[Mh] Termos MeSH primário: Hidrocarboneto de Aril Hidroxilases/metabolismo
Carcinógenos/toxicidade
Citocromo P-450 CYP2E1/biossíntese
Diabetes Mellitus Experimental/enzimologia
Rim/enzimologia
Pulmão/enzimologia
Nitrosaminas/toxicidade
[Mh] Termos MeSH secundário: Aloxano
Anilina Hidroxilase/biossíntese
Animais
Western Blotting
Família 2 do Citocromo P450
Diabetes Mellitus Experimental/etiologia
Dimetilnitrosamina
Eletroforese em Gel de Poliacrilamida
Indução Enzimática
Rim/efeitos dos fármacos
Pulmão/efeitos dos fármacos
Masculino
Microssomos Hepáticos/efeitos dos fármacos
Microssomos Hepáticos/enzimologia
Oxigenases/biossíntese
Coelhos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carcinogens); 0 (Nitrosamines); 6SW5YHA5NG (Alloxan); EC 1.13.- (Oxygenases); EC 1.14.13.- (4-nitrophenol monooxygenase); EC 1.14.13.- (Cytochrome P-450 CYP2E1); EC 1.14.14.- (Aniline Hydroxylase); EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases); EC 1.14.14.1 (Cytochrome P450 Family 2); EC 1.14.14.1 (cytochrome P-450 CYP2B4 (rabbit)); M43H21IO8R (Dimethylnitrosamine)
[Em] Mês de entrada:0702
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:061013
[St] Status:MEDLINE



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