Base de dados : MEDLINE
Pesquisa : D08.244.453.489 [Categoria DeCS]
Referências encontradas : 5 [refinar]
Mostrando: 1 .. 5   no formato [Detalhado]

página 1 de 1

  1 / 5 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28337462
[Au] Autor:Ciesiólka S; Budna J; Jopek K; Bryja A; Kranc W; Borys S; Jeseta M; Chachula A; Ziólkowska A; Antosik P; Bukowska D; Brüssow KP; Bruska M; Nowicki M; Zabel M; Kempisty B
[Ad] Endereço:Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781 Poznan, Poland.
[Ti] Título:Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells.
[So] Source:Biomed Res Int;2017:9738640, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation and transformation of granulosa cells (GCs) during folliculogenesis, oogenesis, and oocyte maturation. Although the role of 17 beta-estradiol (E2) in ovarian activity is widely known, its effect on proliferative capacity, gap junction connection (GJC) formation, and GCs-luteal cells transformation requires further research. Therefore, the goal of this study was to assess the real-time proliferative activity of porcine GCs in vitro in relation to connexin (Cx), luteinizing hormone receptor (LHR), follicle stimulating hormone receptor (FSHR), and aromatase (CYP19A1) expression during short-term (168 h) primary culture. The cultured GCs were exposed to acute (at 96 h of culture) and/or prolonged (between 0 and 168 h of culture) administration of 1.8 and 3.6 M E2. The relative abundance of Cx36, Cx37, Cx40, Cx43, LHR, FSHR, and CYP19A1 mRNA was measured. We conclude that the proliferation capability of GCs in vitro is substantially associated with expression of Cxs, LHR, FSHR, and CYP19A1. Furthermore, the GC-luteal cell transformation in vitro may be significantly accompanied by the proliferative activity of GCs in pigs.
[Mh] Termos MeSH primário: Diferenciação Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células da Granulosa/metabolismo
Oogênese/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular/genética
Família 19 do Citocromo P450/biossíntese
Estradiol/administração & dosagem
Feminino
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Células da Granulosa/efeitos dos fármacos
Seres Humanos
Técnicas de Maturação in Vitro de Oócitos
Oócitos/efeitos dos fármacos
Oócitos/crescimento & desenvolvimento
Oogênese/genética
Receptores do FSH/biossíntese
Receptores do FSH/genética
Receptores do LH/biossíntese
Receptores do LH/genética
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, FSH); 0 (Receptors, LH); 4TI98Z838E (Estradiol); EC 1.14.14.1 (Cytochrome P450 Family 19)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1155/2017/9738640


  2 / 5 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27973579
[Au] Autor:Zhao F; Wang N; Yi Y; Lin P; Tang K; Wang A; Jin Y
[Ad] Endereço:Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.
[Ti] Título:Knockdown of CREB3/Luman by shRNA in Mouse Granulosa Cells Results in Decreased Estradiol and Progesterone Synthesis and Promotes Cell Proliferation.
[So] Source:PLoS One;11(12):e0168246, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Luman (also known as LZIP or CREB3) is a transcription factor and a member of the cAMP responsive element-binding (CREB) family proteins. Although Luman has been detected in apoptotic granulosa cells and disorganized atretic bodies, the physiological function of Luman in follicular development has not been reported. Our objective is to determine the role of Luman in folliculogenesis by knocking down Luman expression in mouse GCs (granulosa cells) using shRNA. Luman expression was successfully knocked down in mouse GCs at the mRNA and protein level, as confirmed by real-time quantitative PCR, western blot and immunofluorescence staining, respectively. Knockdown of Luman significantly decreased the concentrations of estradiol (E2) and progesterone (P4) in cell culture medium. Furthermore, Luman knockdown promoted cell proliferation but had no effect on cell apoptosis. To elucidate the regulatory mechanism underlying the effects of Luman knockdown on steroid synthesis and cell cycle, we measured the mRNA and protein expression levels of several related genes. The expression of Star, Cyp19a1, and Cyp1b1, which encode steroidogenic enzymes, was down-regulated, while that of Cyp11a1 and Runx2, which also encode steroidogenic enzymes, was up-regulated. The expression of the cell cycle factors Cyclin A1, Cyclin B1, Cyclin D2, and Cyclin E was significantly up-regulated. Among apoptosis-related genes, only Bcl-2 was down-regulated, while Caspase 3, Bax and p53 were not significantly affected, suggesting that Luman knockdown may regulate cell cycle activity and hormone secretion at the transcriptional and translational level in mouse GCs. The expression of two important genes associated with folliculogenesis in mouse GCs, Has2 and Ptgs2, were also significantly altered by Luman knockdown. In conclusion, the findings of this study indicate that Luman regulates mouse GCs modulation of steroid synthesis, cell cycle activity and other regulators of folliculogenesis.
[Mh] Termos MeSH primário: Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/genética
Estradiol/biossíntese
Células da Granulosa/citologia
Progesterona/biossíntese
RNA Interferente Pequeno/genética
[Mh] Termos MeSH secundário: Animais
Apoptose
Ciclo Celular
Proliferação Celular
Separação Celular
Citocromo P-450 CYP1B1/metabolismo
Família 19 do Citocromo P450/metabolismo
Feminino
Citometria de Fluxo
Técnicas de Silenciamento de Genes
Células da Granulosa/metabolismo
Lentivirus/metabolismo
Camundongos
Microscopia Confocal
Fosfoproteínas/metabolismo
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Creb3 protein, mouse); 0 (Cyclic AMP Response Element-Binding Protein); 0 (Phosphoproteins); 0 (RNA, Messenger); 0 (RNA, Small Interfering); 0 (steroidogenic acute regulatory protein); 4G7DS2Q64Y (Progesterone); 4TI98Z838E (Estradiol); EC 1.14.14.1 (Cyp1b1 protein, mouse); EC 1.14.14.1 (Cytochrome P-450 CYP1B1); EC 1.14.14.1 (Cytochrome P450 Family 19)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0168246


  3 / 5 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27846500
[Au] Autor:Dydio P; Key HM; Nazarenko A; Rha JY; Seyedkazemi V; Clark DS; Hartwig JF
[Ad] Endereço:Department of Chemistry, University of California, Berkeley, CA 94720, USA.
[Ti] Título:An artificial metalloenzyme with the kinetics of native enzymes.
[So] Source:Science;354(6308):102-106, 2016 10 07.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Natural enzymes contain highly evolved active sites that lead to fast rates and high selectivities. Although artificial metalloenzymes have been developed that catalyze abiological transformations with high stereoselectivity, the activities of these artificial enzymes are much lower than those of natural enzymes. Here, we report a reconstituted artificial metalloenzyme containing an iridium porphyrin that exhibits kinetic parameters similar to those of natural enzymes. In particular, variants of the P450 enzyme CYP119 containing iridium in place of iron catalyze insertions of carbenes into C-H bonds with up to 98% enantiomeric excess, 35,000 turnovers, and 2550 hours turnover frequency. This activity leads to intramolecular carbene insertions into unactivated C-H bonds and intermolecular carbene insertions into C-H bonds. These results lift the restrictions on merging chemical catalysis and biocatalysis to create highly active, productive, and selective metalloenzymes for abiological reactions.
[Mh] Termos MeSH primário: Biocatálise
Família 19 do Citocromo P450/química
Metaloproteínas/química
[Mh] Termos MeSH secundário: Família 19 do Citocromo P450/genética
Irídio/química
Cinética
Metaloproteínas/genética
Metano/análogos & derivados
Metano/química
Mutação
Porfirinas/química
Conformação Proteica
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Metalloproteins); 0 (Porphyrins); 2465-56-7 (carbene); 44448S9773 (Iridium); EC 1.14.14.1 (Cytochrome P450 Family 19); OP0UW79H66 (Methane)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170323
[Lr] Data última revisão:
170323
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161116
[St] Status:MEDLINE


  4 / 5 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27049656
[Au] Autor:Kassem ME; Ibrahim LF; Hussein SR; El-Sharawy R; El-Ansari MA; Hassanane MM; Booles HF
[Ad] Endereço:a Department of Phytochemistry and Plant Systematics , National Research Centre , Dokki , Cairo , Egypt.
[Ti] Título:Myricitrin and bioactive extract of Albizia amara leaves: DNA protection and modulation of fertility and antioxidant-related genes expression.
[So] Source:Pharm Biol;54(11):2404-2409, 2016 Nov.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Albizia species are reported to exhibit many biological activities including antiovulatory properties in female rats and antispermatogenic and antiandrogenic activities in male rats. OBJECTIVE: The present study investigates the flavonoids of Albizia amara (Roxb.) B. Boivin (Fabaceae) leaves and evaluates their activity on gene expression of fertility and antioxidant glutathione-S-transferase-related genes of treated female mice in addition to their effect on DNA damage. MATERIALS AND METHODS: Plant materials were extracted by using 70% methanol for 48 h, the extract was chromatographed on a polyamide 6S column, each isolated compound was purified by using Sephadex LH-20 column; its structure was elucidated by chemical and spectral methods. Both the leaves extract and myricitrin (200, 30 mg/kg bw/d, respectively) were assayed for their effect on DNA damage in female mice after four weeks treatment using Comet assay. Their modulatory activity on gene expression of fertility (aromatase CYP19 and luteinizing hormone LH) and antioxidant glutathione-S-transferase (GST)-related genes of treated female mice were investigated by real-time PCR (qPCR). RESULTS: Quercetin-3-O-gentiobioside, myricitrin, quercetin-3-O-α-rhamnopyranoside, myricetin, quercetin and kaempferol were isolated and identified from the studied taxa. Myricitrin and the extract induced low rate of DNA damage (4.8% and 5%, respectively), compared with the untreated control (4.2%) and significantly down-regulated the expression of CYP19 and LH genes and up-regulated GST gene. DISCUSSION AND CONCLUSION: Our results highlight the potential effect of the leaves extract of Albizia amara and myricitrin as fertility-regulating phytoconstituents with ability to protect DNA from damage and cells from oxidative stress.
[Mh] Termos MeSH primário: Albizzia/química
Dano ao DNA/efeitos dos fármacos
Fertilidade/efeitos dos fármacos
Flavonoides/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Família 19 do Citocromo P450/genética
Feminino
Flavonoides/isolamento & purificação
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Glutationa Transferase/genética
Camundongos
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 0 (Plant Extracts); 5Z0ZO61WPJ (myricitrin); EC 1.14.14.1 (Cytochrome P450 Family 19); EC 2.5.1.18 (Glutathione Transferase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170303
[Lr] Data última revisão:
170303
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160407
[St] Status:MEDLINE


  5 / 5 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26893163
[Au] Autor:Chatuphonprasert W; Jarukamjorn K; Putalun W
[Ad] Endereço:Faculty of Medicine, Mahasarakham University, Maha Sarakham, Thailand.
[Ti] Título:Regulation of cancer-related genes - Cyp1a1, Cyp1b1, Cyp19, Nqo1 and Comt - expression in ß-naphthoflavone-treated mice by miroestrol.
[So] Source:J Pharm Pharmacol;68(4):475-84, 2016 Apr.
[Is] ISSN:2042-7158
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The effects of miroestrol (MR), an active phytoestrogen from Pueraria candollei var. mirifica, on expression of cancer-related genes were determined. METHODS: Seven-week-old female ICR mice (n = 5 each) were subcutaneously administered estradiol (E2, 0.5 mg/kg/day) or MR (0.5 or 5 mg/kg/day) daily for 7 days. Some were given ER or MR in combination with ß-naphthoflavone (BNF, 30 mg/kg/day) for the last 3 days. The expression of cancer-related genes including cytochrome P450 1A (Cyp1a), cytochrome P450 1B1 (Cyp1b1), aromatase P450 (Cyp19), NAD(P)H: quinone oxidoreductase 1 (Nqo1) and catechol-O-methyltransferase (Comt) were evaluated. KEY FINDINGS: In the presence of BNF, MR suppressed hepatic CYP1A1 activity and CYP1A2 activity, expression of CYP1B1 mRNA and expression of CYP1A1/2 and CYP1B1 protein. E2, by contrast, did not. MR restored expression levels of hepatic NQO1 and uterine COMT in BNF-treated mice. Furthermore, MR increased expression of uterine CYP19 to the same extent as E2. CONCLUSION: MR may be superior to E2 as it downregulates expression of CYP1. Moreover, MR normalized expression of both NQO1 and COMT, the protective enzymes, in murine liver and uteri. These results support the use of MR as an alternative supplement for menopausal women, MR having the extra benefit of reducing cancer risk.
[Mh] Termos MeSH primário: Catecol O-Metiltransferase/metabolismo
Citocromo P-450 CYP1A1/metabolismo
Citocromo P-450 CYP1B1/metabolismo
Família 19 do Citocromo P450/metabolismo
Terapia de Reposição de Estrogênios/métodos
Fígado/efeitos dos fármacos
NAD(P)H Desidrogenase (Quinona)/metabolismo
Fitoestrógenos/farmacologia
Esteroides/farmacologia
Útero/efeitos dos fármacos
beta-Naftoflavona/farmacologia
[Mh] Termos MeSH secundário: Animais
Catecol O-Metiltransferase/genética
Citocromo P-450 CYP1A1/genética
Citocromo P-450 CYP1B1/genética
Família 19 do Citocromo P450/genética
Estradiol/farmacologia
Terapia de Reposição de Estrogênios/efeitos adversos
Feminino
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Fígado/enzimologia
Camundongos Endogâmicos ICR
NAD(P)H Desidrogenase (Quinona)/genética
Neoplasias/induzido quimicamente
Neoplasias/enzimologia
Neoplasias/genética
Neoplasias/prevenção & controle
Fitoestrógenos/toxicidade
Esteroides/toxicidade
Útero/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phytoestrogens); 0 (Steroids); 0 (miroestrol); 4TI98Z838E (Estradiol); 6051-87-2 (beta-Naphthoflavone); EC 1.14.14.1 (Cyp1b1 protein, mouse); EC 1.14.14.1 (Cytochrome P-450 CYP1A1); EC 1.14.14.1 (Cytochrome P-450 CYP1B1); EC 1.14.14.1 (Cytochrome P450 Family 19); EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone)); EC 1.6.5.2 (Nqo1 protein, mouse); EC 2.1.1.6 (COMT protein, mouse); EC 2.1.1.6 (Catechol O-Methyltransferase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160220
[St] Status:MEDLINE
[do] DOI:10.1111/jphp.12531



página 1 de 1
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde