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Pesquisa : D08.244.453.880 [Categoria DeCS]
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[PMID]:28463435
[Au] Autor:Peng T; Chen X; Pan Y; Zheng Z; Wei X; Xi J; Zhang J; Gao X; Shang Q
[Ad] Endereço:College of Plant Science, Jilin University, Changchun, China.
[Ti] Título:Transcription factor aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator is involved in regulation of the xenobiotic tolerance-related cytochrome P450 CYP6DA2 in Aphis gossypii Glover.
[So] Source:Insect Mol Biol;26(5):485-495, 2017 10.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The cotton aphid, Aphis gossypii, is one of the most economically important agricultural pests worldwide as it is polyphagous and resistant to many classes of insecticides. Overexpression of the cytochrome P450 monooxygenase (P450) CYP6DA2 has previously been found to be associated with gossypol and spirotetramat tolerance in the cotton aphid. In the present study, the elements located in the promoter region (-357:-343; -250:-241; -113:-104) of CYP6DA2 were shown to control promoter activity, and gossypol induction was observed. We hypothesized that the expression of CYP6DA2 is subject to transcriptional regulation. To investigate the underlying mechanism, we assessed two transcription factors, aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT), and found that the abundance of AhR was highly correlated with CYP6DA2 abundance. RNA interference of AhR or ARNT significantly decreased the levels of the target gene as well as those of its counterpart, and both dramatically repressed CYP6DA2 expression. Cotransfection of the ARNT, AhR, or AhR plus ARNT and CYP6DA2 promoter constructs elevated CYP6DA2 promoter activity, with the AhR plus ARNT cotransfection being the most effective. Thus, these elements located in the promoter were responsible for CYP6DA2 transcription, and CYP6DA2 expression was regulated by the transcription factors AhR and ARNT.
[Mh] Termos MeSH primário: Afídeos/metabolismo
Translocador Nuclear Receptor Aril Hidrocarboneto/metabolismo
Família 6 do Citocromo P450/metabolismo
Receptores de Hidrocarboneto Arílico/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Afídeos/genética
Compostos Aza
Sequência de Bases
Sequência Conservada
Família 6 do Citocromo P450/genética
Técnicas de Silenciamento de Genes
Gossipol
Proteínas de Insetos/metabolismo
Resistência a Inseticidas
Dados de Sequência Molecular
Regiões Promotoras Genéticas
Compostos de Espiro
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aza Compounds); 0 (Insect Proteins); 0 (Receptors, Aryl Hydrocarbon); 0 (Spiro Compounds); 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator); 4G7KR034OX (spirotetramat); EC 1.14.14.1 (Cytochrome P450 Family 6); KAV15B369O (Gossypol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12311


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[PMID]:28456304
[Au] Autor:Tian K; Liu D; Yuan Y; Li M; Qiu X
[Ad] Endereço:State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
[Ti] Título:CYP6B6 is involved in esfenvalerate detoxification in the polyphagous lepidopteran pest, Helicoverpa armigera.
[So] Source:Pestic Biochem Physiol;138:51-56, 2017 May.
[Is] ISSN:1095-9939
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The cotton bollworm, Helicoverpa armigera, is a polyphagous pest that has a strong capacity to evolve resistance against various classes of insecticides. Cytochrome P450 enzymes have been suspected involved in pyrethroid metabolism and resistance in this pest. However, how many and which P450s are involved in pyrethroid metabolism is largely unknown. In this study, CYP6B6 and NADPH-cytochrome P450 reductase (HaCPR) from H. armigera were successfully co-expressed in Escherichia coli. Incubation of esfenvalerate with the recombinant CYP6B6-HaCPR monooxygenase complex revealed that CYP6B6 was able to transform esfenvalerate into 4'-hydroxy fenvalerate. Kcat and Km values for the formation of 4'-hydroxyfenvalerate by the E. coli-produced CYP6B6 were determined to be 1.65±0.11min and 4.10±0.84µM respectively. Our results demonstrate that CYP6B6 has the ability to hydroxylate esfenvalerate, thus plays a role in fenvalerate detoxification.
[Mh] Termos MeSH primário: Família 6 do Citocromo P450/metabolismo
Proteínas de Insetos/metabolismo
Inseticidas/metabolismo
Mariposas/efeitos dos fármacos
Mariposas/enzimologia
Nitrilos/metabolismo
Piretrinas/metabolismo
[Mh] Termos MeSH secundário: Animais
Família 6 do Citocromo P450/genética
Regulação Enzimológica da Expressão Gênica/fisiologia
Proteínas de Insetos/genética
Resistência a Inseticidas
Inseticidas/química
Inseticidas/farmacologia
Estrutura Molecular
Nitrilos/química
Nitrilos/farmacologia
Piretrinas/química
Piretrinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Insecticides); 0 (Nitriles); 0 (Pyrethrins); EC 1.14.14.1 (Cytochrome P450 Family 6); Z6MXZ39302 (fenvalerate)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


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[PMID]:28654199
[Au] Autor:Wang R; Zhu Y; Deng L; Zhang H; Wang Q; Yin M; Song P; Elzaki MEA; Han Z; Wu M
[Ad] Endereço:Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, Jiangsu, China.
[Ti] Título:Imidacloprid is hydroxylated by Laodelphax striatellus CYP6AY3v2.
[So] Source:Insect Mol Biol;26(5):543-551, 2017 Oct.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Laodelphax striatellus (Fallén) is one of the most destructive pests of rice, and has developed high resistance to imidacloprid. Our previous work indicated a strong association between imidacloprid resistance and the overexpression of a cytochrome P450 gene CYP6AY3v2 in a L. striatellus imidacloprid resistant strain (Imid-R). In this study, a transgenic Drosophila melanogaster line that overexpressed the L. striatellus CYP6AY3v2 gene was established and was found to confer increased levels of imidacloprid resistance. Furthermore, CYP6AY3v2 was co-expressed with D. melanogaster cytochrome P450 reductase (CPR) in Spodoptera frugiperda 9 (SF9) cells. A carbon monoxide difference spectra analysis indicated that CYP6AY3v2 was expressed predominately in its cytochrome P450 (P450) form, which is indicative of a good-quality functional enzyme. The recombinant CYP6AY3v2 protein efficiently catalysed the model substrate P-nitroanisole to p-nitrophenol with a maximum velocity (V ) of 60.78 ± 3.93 optical density (mOD)/min/mg protein. In addition, imidacloprid itself was metabolized by the recombinant CYP6AY3v2/nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt (NADPH) CPR microsomes in in vitro assays (catalytic constant (K ) = 0.34 pmol/min/pmol P450, michaelis constant (K ) = 41.98 µM), and imidacloprid depletion and metabolite peak formation were with a time dependence. The data provided direct evidence that CYP6AY3v2 is capable of hydroxylation of imidacloprid and conferring metabolic resistance in L. striatellus.
[Mh] Termos MeSH primário: Família 6 do Citocromo P450/metabolismo
Hemípteros/enzimologia
Imidazóis/metabolismo
Inseticidas/metabolismo
Nitrocompostos/metabolismo
[Mh] Termos MeSH secundário: Animais
Feminino
Hidroxilação
Proteínas de Insetos/metabolismo
Neonicotinoides
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Imidazoles); 0 (Insect Proteins); 0 (Insecticides); 0 (Neonicotinoids); 0 (Nitro Compounds); 3BN7M937V8 (imidacloprid); EC 1.14.14.1 (Cytochrome P450 Family 6)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12317


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[PMID]:28251870
[Au] Autor:Konorov EA; Nikitin MA; Mikhailov KV; Lysenkov SN; Belenky M; Chang PL; Nuzhdin SV; Scobeyeva VA
[Ad] Endereço:Faculty of Biology, Department of Evolution, Lomonosov Moscow State University, Moscow, Russian Federation.
[Ti] Título:Genomic exaptation enables Lasius niger adaptation to urban environments.
[So] Source:BMC Evol Biol;17(Suppl 1):39, 2017 Feb 07.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The world is rapidly urbanizing, and only a subset of species are able to succeed in stressful city environments. Efficient genome-enabled stress response appears to be a likely prerequisite for urban adaptation. Despite the important role ants play in the ecosytem, only the genomes of ~13 have been sequenced so far. Here, we present the draft genome assembly of the black garden ant Lasius niger - the most successful urban inhabitant of all ants - and we compare it with the genomes of other ant species, including the closely related Camponotus floridanus. RESULTS: Sequences from 272 M Illumina reads were assembled into 41,406 contigs with total length of 245 MB, and N50 of 16,382 bp, similar to other ant genome assemblies enabling comparative genomic analysis. Remarkably, the predicted proteome of L. niger is significantly enriched relative to other ant genomes in terms of abundance of domains involved in nucleic acid binding, DNA repair, and nucleotidyl transferase activity, reflecting transposable element proliferation and a likely genomic response. With respect to environmental stress, we note a proliferation of various detoxification genes, including glutatione-S-transferases and those in the cytochrome P450 families. Notably, the CYP9 family is highly expanded with 19 complete and 21 nearly complete members - over twice as many compared to other ants. This family exhibits the signatures of strong directional selection, with eleven positively selected positions in ligand-binding pockets of enzymes. Gene family contraction was detected for several components of the olfactory system, accompanied by instances of both directional selection and relaxation. CONCLUSIONS: Our results suggest that the success of L. niger in urbanized areas may be the result of fortuitous coincidence of several factors, including the expansion of the CYP9 cytochrome family due to coevolution with parasitic fungi, the diversification of DNA repair systems as an answer to proliferation of retroelements, and the reduction of olfactory system and behavioral preadaptations from non-territorial subdominant life strategies found in natural environments. Diversification of cytochromes and DNA repair systems along with reduced odorant communication are the basis of L. niger pollutant resistance and polyphagy, while non-territorial and mobilization strategies allows more efficient exploitation of large but patchy food sources.
[Mh] Termos MeSH primário: Formigas/genética
[Mh] Termos MeSH secundário: Aclimatação
Adaptação Fisiológica
Animais
Formigas/enzimologia
Formigas/microbiologia
Formigas/fisiologia
Sequência de Bases
Sistema Enzimático do Citocromo P-450/química
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Família 6 do Citocromo P450/química
Família 6 do Citocromo P450/genética
Família 6 do Citocromo P450/metabolismo
Elementos de DNA Transponíveis
Fungos/genética
Genoma de Inseto
Genômica
Modelos Moleculares
Anotação de Sequência Molecular
Receptores Odorantes/química
Receptores Odorantes/genética
Receptores Odorantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (Receptors, Odorant); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.14.1 (Cytochrome P450 Family 6)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1186/s12862-016-0867-x


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[PMID]:27687846
[Au] Autor:Krempl C; Heidel-Fischer HM; Jiménez-Alemán GH; Reichelt M; Menezes RC; Boland W; Vogel H; Heckel DG; Joußen N
[Ad] Endereço:Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Str. 8, 07745 Jena, Germany. Electronic address: ckrempl@ice.mpg.de.
[Ti] Título:Gossypol toxicity and detoxification in Helicoverpa armigera and Heliothis virescens.
[So] Source:Insect Biochem Mol Biol;78:69-77, 2016 Nov.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Gossypol is a polyphenolic secondary metabolite produced by cotton plants, which is toxic to many organisms. Gossypol's aldehyde groups are especially reactive, forming Schiff bases with amino acids of proteins and cross-linking them, inhibiting enzyme activities and contributing to toxicity. Very little is known about gossypol's mode of action and its detoxification in cotton-feeding insects that can tolerate certain concentrations of this compound. Here, we tested the toxicity of gossypol and a gossypol derivative lacking free aldehyde groups (SB-gossypol) toward Helicoverpa armigera and Heliothis virescens, two important pests on cotton plants. Larval feeding studies with these two species on artificial diet supplemented with gossypol or SB-gossypol revealed no detectable toxicity of gossypol, when the aldehyde groups were absent. A cytochrome P450 enzyme, CYP6AE14, is upregulated in H. armigera feeding on gossypol, and has been claimed to directly detoxify gossypol. However, using in vitro assays with heterologously expressed CYP6AE14, no metabolites of gossypol were detected, and further studies suggest that gossypol is not a direct substrate of CYP6AE14. Furthermore, larvae feeding on many other plant toxins also upregulate CYP6AE14. Our data demonstrate that the aldehyde groups are critical for the toxicity of gossypol when ingested by H. armigera and H. virescens larvae, and suggest that CYP6AE14 is not directly involved in gossypol metabolism, but may play a role in the general stress response of H. armigera larvae toward plant toxins.
[Mh] Termos MeSH primário: Família 6 do Citocromo P450/genética
Gossipol/metabolismo
Proteínas de Insetos/genética
Larva/metabolismo
Mariposas/metabolismo
[Mh] Termos MeSH secundário: Animais
Família 6 do Citocromo P450/metabolismo
Inativação Metabólica
Proteínas de Insetos/metabolismo
Larva/crescimento & desenvolvimento
Mariposas/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); EC 1.14.14.1 (Cytochrome P450 Family 6); KAV15B369O (Gossypol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161001
[St] Status:MEDLINE


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[PMID]:27651043
[Au] Autor:Tian M; Liu B; Hu H; Li X; Guo Q; Zou F; Liu X; Hu M; Guo J; Ma L; Zhou D; Sun Y; Shen B; Zhu C
[Ad] Endereço:Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
[Ti] Título:MiR-285 targets P450 (CYP6N23) to regulate pyrethroid resistance in Culex pipiens pallens.
[So] Source:Parasitol Res;115(12):4511-4517, 2016 Dec.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:MicroRNAs play critical roles in post-transcriptional regulation of gene expression, which participate in the modulation of almost all of the cellular processes. Although emerging evidence indicates that microRNAs are related with antineoplastic drugs resistance, whether microRNAs are responsible for insecticide resistance in mosquitos is poorly understood. In this paper, we found that miR-285 was significantly upregulated in the deltamethrin-resistant strain of Culex pipiens pallens, and overexpression miR-285 through microinjection increased mosquito survival rate against deltamethrin treatement. Using bioinformatic software, quantitative reverse transcription PCR, luciferase reporter assay and microinjection approaches, we conformed that CYP6N23 was the target of miR-285. Lower expression of CYP6N23 was observed in the deltamethrin-resistant strain. While, mosquito mortality rate was decreased after downregulating expression of CYP6N23 by dsRNA against CYP6N23 or miR-285 mimic microinjection. These findings revealed that miR-285 could target CYP6N23 to regulate pyrethroid resistance, providing new insights into mosquito insecticide resistance surveillance and control.
[Mh] Termos MeSH primário: Culex/efeitos dos fármacos
Culex/enzimologia
Família 6 do Citocromo P450/genética
Proteínas de Insetos/genética
Inseticidas/farmacologia
MicroRNAs/genética
[Mh] Termos MeSH secundário: Animais
Culex/genética
Culex/metabolismo
Família 6 do Citocromo P450/metabolismo
Regulação da Expressão Gênica/efeitos dos fármacos
Proteínas de Insetos/metabolismo
Resistência a Inseticidas
MicroRNAs/metabolismo
Nitrilos/farmacologia
Piretrinas/farmacologia
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Insecticides); 0 (MicroRNAs); 0 (Nitriles); 0 (Pyrethrins); 2JTS8R821G (decamethrin); EC 1.14.14.1 (Cytochrome P450 Family 6)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160922
[St] Status:MEDLINE


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[PMID]:27318250
[Au] Autor:Peng T; Pan Y; Gao X; Xi J; Zhang L; Ma K; Wu Y; Zhang J; Shang Q
[Ad] Endereço:College of Plant Science, Jilin University, Changchun, 130062, PR China.
[Ti] Título:Reduced abundance of the CYP6CY3-targeting let-7 and miR-100 miRNAs accounts for host adaptation of Myzus persicae nicotianae.
[So] Source:Insect Biochem Mol Biol;75:89-97, 2016 Aug.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nicotine is one of the most abundant and toxic secondary plant metabolites in nature and is defined by high toxicity to plant-feeding insects. Studies suggest that increased expression of cytochrome P450 (CYP6CY3) and the homologous CYP6CY4 genes in Myzus persicae nicotianae is correlated with tolerance to nicotine. Indeed, through expression analyses of the CYP6CY3 and CYP6CY4 genes of different M. persicae subspecies, we determined that the mRNA levels of these two genes were much higher in M. persicae nicotianae than in M. persicae sensu stricto. We hypothesized that the expression of these two genes is subject to post-transcriptional regulation. To investigate the underlying mechanism, the miRNA profile of M. persicae nicotianae was sequenced, and twenty-two miRNAs were predicted to target CYP6CY3. Validation of these miRNAs identified two miRNAs, let-7 and miR-100, whose abundance was highly inversely correlated with the abundance of the CYP6CY3 gene. This result implies that the let-7 and miR-100 miRNAs play a major role in the post-transcriptional regulation of the CYP6CY3 gene. Modulation of the abundance of let-7 and miR-100 through the addition of inhibitors/mimics of let-7 or miR-100 to artificial diet significantly altered the tolerance of M. persicae nicotianae to nicotine, further confirming the regulatory role of these two miRNAs. Interestingly, after decreasing the transcript levels of CYP6CY3 by modulating regulatory miRNAs, the transcript levels of the homologous isozyme CYP6CY4 were significantly elevated, suggesting a compensatory mechanism between the CYP6CY3 gene and its homologous CYP6CY4 gene. Our findings provide insight into the molecular drivers of insect host shifts and reveal an important source of genetic variation for adaptive evolution in insect species.
[Mh] Termos MeSH primário: Afídeos/fisiologia
Família 6 do Citocromo P450/genética
Herbivoria
Proteínas de Insetos/genética
MicroRNAs/genética
Tabaco
[Mh] Termos MeSH secundário: Animais
Afídeos/genética
Família 6 do Citocromo P450/metabolismo
Cadeia Alimentar
Proteínas de Insetos/metabolismo
MicroRNAs/metabolismo
Análise de Sequência de RNA
Tabaco/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (MicroRNAs); EC 1.14.14.1 (Cytochrome P450 Family 6)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160619
[St] Status:MEDLINE


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[PMID]:27094778
[Au] Autor:Ishak IH; Riveron JM; Ibrahim SS; Stott R; Longbottom J; Irving H; Wondji CS
[Ad] Endereço:Department of Vector Biology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, United Kingdom.
[Ti] Título:The Cytochrome P450 gene CYP6P12 confers pyrethroid resistance in kdr-free Malaysian populations of the dengue vector Aedes albopictus.
[So] Source:Sci Rep;6:24707, 2016 Apr 20.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Control of Aedes albopictus, major dengue and chikungunya vector, is threatened by growing cases of insecticide resistance. The mechanisms driving this resistance remain poorly characterised. This study investigated the molecular basis of insecticide resistance in Malaysian populations of Ae. albopictus. Microarray-based transcription profiling revealed that metabolic resistance (cytochrome P450 up-regulation) and possibly a reduced penetration mechanism (consistent over-expression of cuticular protein genes) were associated with pyrethroid resistance. CYP6P12 over-expression was strongly associated with pyrethroid resistance whereas CYP6N3 was rather consistently over-expressed across carbamate and DDT resistant populations. Other detoxification genes also up-regulated in permethrin resistant mosquitoes included a glucuronosyltransferase (AAEL014279-RA) and the glutathione-S transferases GSTS1 and GSTT3. Functional analyses further supported that CYP6P12 contributes to pyrethroid resistance in Ae. albopictus as transgenic expression of CYP6P12 in Drosophila was sufficient to confer pyrethroid resistance in these flies. Furthermore, molecular docking simulations predicted CYP6P12 possessing enzymatic activity towards pyrethroids. Patterns of polymorphism suggested early sign of selection acting on CYP6P12 but not on CYP6N3. The major role played by P450 in the absence of kdr mutations suggests that addition of the synergist PBO to pyrethroids could improve the efficacy of this insecticide class and overcome resistance in field populations of Ae. albopictus.
[Mh] Termos MeSH primário: Aedes/efeitos dos fármacos
Aedes/genética
Família 6 do Citocromo P450/genética
Resistência a Inseticidas/genética
Inseticidas/farmacologia
Piretrinas/farmacologia
[Mh] Termos MeSH secundário: Aedes/virologia
Animais
Animais Geneticamente Modificados
Família 6 do Citocromo P450/química
Dengue/transmissão
Perfilação da Expressão Gênica
Regulação da Expressão Gênica
Técnicas de Silenciamento de Genes
Genética Populacional
Inseticidas/química
Malásia
Modelos Moleculares
Conformação Molecular
Polimorfismo Genético
Ligação Proteica
Piretrinas/química
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insecticides); 0 (Pyrethrins); EC 1.14.14.1 (Cytochrome P450 Family 6)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160421
[St] Status:MEDLINE
[do] DOI:10.1038/srep24707


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[PMID]:26853074
[Au] Autor:Guo Y; Wu H; Zhang X; Ma E; Guo Y; Zhu KY; Zhang J
[Ad] Endereço:Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, China.
[Ti] Título:RNA interference of cytochrome P450 CYP6F subfamily genes affects susceptibility to different insecticides in Locusta migratoria.
[So] Source:Pest Manag Sci;72(11):2154-2165, 2016 Nov.
[Is] ISSN:1526-4998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Many insect cytochrome P450s (CYPs) play critical roles in detoxification of insecticides. The CYP6 family is unique to the class Insecta, and its biochemical function has essentially been associated with the metabolism of xenobiotics. In this study, we sequenced and characterised the full-length cDNAs of five CYP genes from Locusta migratoria, a highly destructive agricultural pest worldwide. RESULTS: The five genes were predominantly expressed in brain, guts, fat bodies or Malpighian tubules. CYP6FE1, CYP6FF1 and CYP6FG1 were expressed at higher levels in fourth-instar nymphs than in other developmental stages. CYPFD2 is specifically expressed in adults, whereas CYP6FD1, CYP6FD2 and CYP6FE1 showed significantly lower expression in eggs than in other developmental stages. Deltamethrin suppressed CYP6FD1 expression in third-instar nymphs and upregulated the expression level of CYP6FD2, CYP6FF1 and CYP6FG1 at the dose of LD . Efficient RNA interference-mediated gene silencing was established for four of the five CYP genes. Silencing of CYP6FF1 increased the nymphal mortality from 23 to 50% in response to deltamethrin. Silencing of CYP6FD2 and CYP6FE1 increased the nymphal mortality from 32 to 72 and 66%, respectively, to carbaryl. CONCLUSION: Three of the four CYP6F subfamily genes in L. migratoria were associated with the detoxification of deltamethrin or carbaryl. The role of CYPs in insecticide detoxification appears to be both gene and insecticide specific. © 2016 Society of Chemical Industry.
[Mh] Termos MeSH primário: Família 6 do Citocromo P450/genética
Proteínas de Insetos/genética
Resistência a Inseticidas
Inseticidas/farmacologia
Locusta migratoria/efeitos dos fármacos
Interferência de RNA
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Família 6 do Citocromo P450/metabolismo
Inativação Metabólica
Proteínas de Insetos/metabolismo
Locusta migratoria/genética
Locusta migratoria/crescimento & desenvolvimento
Locusta migratoria/metabolismo
Ninfa/efeitos dos fármacos
Ninfa/genética
Ninfa/crescimento & desenvolvimento
Ninfa/metabolismo
Especificidade de Órgãos
Filogenia
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Insecticides); EC 1.14.14.1 (Cytochrome P450 Family 6)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160209
[St] Status:MEDLINE
[do] DOI:10.1002/ps.4248


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[PMID]:26696496
[Au] Autor:Zhao J; Liu XN; Li F; Zhuang SZ; Huang LN; Ma J; Gao XW
[Ad] Endereço:Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi,Xinjiang,China.
[Ti] Título:Yeast one-hybrid screening the potential regulator of CYP6B6 overexpression of Helicoverpa armigera under 2-tridecanone stress.
[So] Source:Bull Entomol Res;106(2):182-90, 2016 Apr.
[Is] ISSN:1475-2670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In insect, the cytochrome P450 plays a pivotal role in detoxification to toxic allelochemicals. Helicoverpa armigera can tolerate and survive in 2-tridecanone treatment owing to the CYP6B6 responsive expression, which is controlled by some regulatory DNA sequences and transcription regulators. Therefore, the 2-tridecanone responsive region and transcription regulators of the CYP6B6 are responsible for detoxification of cotton bollworm. In this study, we used yeast one-hybrid to screen two potential transcription regulators of the CYP6B6 from H. armigera that respond to the plant secondary toxicant 2-tridecanone, which were named Prey1 and Prey2, respectively. According to the NCBI database blast, Prey1 is the homology with FK506 binding protein (FKBP) of Manduca sexta and Bombyx mori that belongs to the FKBP-C superfamily, while Prey2 may be a homology of an unknown protein of Papilio or the fcaL24 protein homology of B. mori. The electrophoretic mobility shift assays revealed that the FKBP of prokaryotic expression could specifically bind to the active region of the CYP6B6 promoter. After the 6th instar larvae of H. armigera reared on 2-tridecanone artificial diet, we found there were similar patterns of CYP6B6 and FKBP expression of the cotton bollworm treated with 10 mg g-1 2-tridecanone for 48 h, which correlation coefficient was the highest (0.923). Thus, the FKBP is identified as a strong candidate for regulation of the CYP6B6 expression, when the cotton bollworm is treated with 2-tridecanone. This may lead us to a better understanding of transcriptional mechanism of CYP6B6 and provide very useful information for the pest control.
[Mh] Termos MeSH primário: Família 6 do Citocromo P450/metabolismo
Regulação Enzimológica da Expressão Gênica/fisiologia
Cetonas/farmacologia
Mariposas/fisiologia
[Mh] Termos MeSH secundário: Animais
Família 6 do Citocromo P450/genética
DNA Complementar/genética
Ensaio de Desvio de Mobilidade Eletroforética
Biblioteca Gênica
Genes Reporter
Mariposas/efeitos dos fármacos
Plasmídeos/genética
Regiões Promotoras Genéticas
Reprodutibilidade dos Testes
Estresse Fisiológico
Proteína 1A de Ligação a Tacrolimo/metabolismo
Técnicas do Sistema de Duplo-Híbrido
Leveduras/genética
Leveduras/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Ketones); 5Q35VHX26K (2-tridecanone); EC 1.14.14.1 (Cytochrome P450 Family 6); EC 5.2.1.- (Tacrolimus Binding Protein 1A)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151224
[St] Status:MEDLINE
[do] DOI:10.1017/S0007485315000942



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