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[PMID]:26903405
[Au] Autor:Hou S; Trochimczyk P; Sun L; Wisniewska A; Kalwarczyk T; Zhang X; Wielgus-Kutrowska B; Bzowska A; Holyst R
[Ad] Endereço:Institute of Physical Chemistry PAS, Kasprzaka 44/52, 01-224 Warsaw, Poland.
[Ti] Título:How can macromolecular crowding inhibit biological reactions? The enhanced formation of DNA nanoparticles.
[So] Source:Sci Rep;6:22033, 2016 Feb 23.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In contrast to the already known effect that macromolecular crowding usually promotes biological reactions, solutions of PEG 6k at high concentrations stop the cleavage of DNA by HindIII enzyme, due to the formation of DNA nanoparticles. We characterized the DNA nanoparticles and probed the prerequisites for their formation using multiple techniques such as fluorescence correlation spectroscopy, dynamic light scattering, fluorescence analytical ultracentrifugation etc. In >25% PEG 6k solution, macromolecular crowding promotes the formation of DNA nanoparticles with dimensions of several hundreds of nanometers. The formation of DNA nanoparticles is a fast and reversible process. Both plasmid DNA (2686 bp) and double-stranded/single-stranded DNA fragment (66 bp/nt) can form nanoparticles. We attribute the enhanced nanoparticle formation to the depletion effect of macromolecular crowding. This study presents our idea to enhance the formation of DNA nanoparticles by macromolecular crowding, providing the first step towards a final solution to efficient gene therapy.
[Mh] Termos MeSH primário: DNA/química
Nanopartículas/química
[Mh] Termos MeSH secundário: DNA de Cadeia Simples/química
Desoxirribonuclease HindIII
Substâncias Macromoleculares/química
Plasmídeos/química
Polietilenoglicóis
Espectrometria de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Single-Stranded); 0 (Macromolecular Substances); 30IQX730WE (Polyethylene Glycol 6000); 30IQX730WE (Polyethylene Glycols); 9007-49-2 (DNA); EC 3.1.21.- (Deoxyribonuclease HindIII)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160224
[St] Status:MEDLINE
[do] DOI:10.1038/srep22033


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[PMID]:25664735
[Au] Autor:Kawamura T; Kobayashi T; Watanabe N
[Ad] Endereço:Synchrotron Radiation Research Center, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan.
[Ti] Título:Analysis of the HindIII-catalyzed reaction by time-resolved crystallography.
[So] Source:Acta Crystallogr D Biol Crystallogr;71(Pt 2):256-65, 2015 Feb.
[Is] ISSN:1399-0047
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In order to investigate the mechanism of the reaction catalyzed by HindIII, structures of HindIII-DNA complexes with varying durations of soaking time in cryoprotectant buffer containing manganese ions were determined by the freeze-trap method. In the crystal structures of the complexes obtained after soaking for a longer duration, two manganese ions, indicated by relatively higher electron density, are clearly observed at the two metal ion-binding sites in the active site of HindIII. The increase in the electron density of the two metal-ion peaks followed distinct pathways with increasing soaking times, suggesting variation in the binding rate constant for the two metal sites. DNA cleavage is observed when the second manganese ion appears, suggesting that HindIII uses the two-metal-ion mechanism, or alternatively that its reactivity is enhanced by the binding of the second metal ion. In addition, conformational change in a loop near the active site accompanies the catalytic reaction.
[Mh] Termos MeSH primário: DNA/metabolismo
Desoxirribonuclease HindIII/metabolismo
Haemophilus influenzae/enzimologia
[Mh] Termos MeSH secundário: Cristalização
Cristalografia por Raios X
DNA/química
Desoxirribonuclease HindIII/química
Infecções por Haemophilus/microbiologia
Haemophilus influenzae/química
Haemophilus influenzae/metabolismo
Seres Humanos
Manganês/metabolismo
Modelos Moleculares
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
42Z2K6ZL8P (Manganese); 9007-49-2 (DNA); EC 3.1.21.- (Deoxyribonuclease HindIII)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150210
[St] Status:MEDLINE
[do] DOI:10.1107/S1399004714025188


  3 / 1195 MEDLINE  
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[PMID]:22101375
[Au] Autor:Huang X; Gong R; Lin J; Li R; Xiao L; Duan W; Fang D
[Ad] Endereço:Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
[Ti] Título:Effects of lipoprotein lipase gene variations, a high-carbohydrate low-fat diet, and gender on serum lipid profiles in healthy Chinese Han youth.
[So] Source:Biosci Trends;5(5):198-204, 2011.
[Is] ISSN:1881-7823
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:A high-carbohydrate low-fat (HC/LF) diet and lipoprotein lipase gene (LPL) Ser447Stop and Hind III polymorphisms have separately been found to be associated with triacylglycerol (TG) and high density lipoprotein cholesterol (HDL-C). This study sought to test the effects of LPL polymorphisms and an HC/LF diet on the serum lipid profile of Chinese with a lower incidence of coronary artery disease (CAD) consuming a diet with less fat and more carbohydrates. Fifty-six healthy subjects (22.89 ± 1.80 years) were given a control diet of 30.1% fat and 54.1% carbohydrates for 7 days, followed by an HC/LF diet of 13.8% fat and 70.1% carbohydrate for 6 days; there were no changes in the fatty acid composition or restrictions on total energy. Serum lipid profiles at baseline, before and after the HC/LF diet, and LPL polymorphisms were analyzed. After 6 days of the HC/LF diet, TG and the homeostasis model assessment of insulin resistance (HOMAIR) index were found to increase only in females with S447S. No decrease in HDL-C was noted. In subjects with Hind III polymorphism, increased TG was found in all females but not in males. Increased HDL-C, together with apolipoprotein (apo) AI, was found in male H- carriers but not in males with H+/H+ and females. In conclusion, LPL Ser447Stop and Hind III polymorphisms modified the effects of an HC/LF diet on the serum lipid profiles of a young Chinese population in different ways. Effective strategies for dietary interventions targeted at younger populations should take into account the interplay between genetic polymorphisms, diet, and gender.
[Mh] Termos MeSH primário: Grupo com Ancestrais do Continente Asiático/genética
Dieta com Restrição de Gorduras
Carboidratos da Dieta/farmacologia
Lipídeos/sangue
Lipase Lipoproteica/genética
Polimorfismo de Nucleotídeo Único/genética
Caracteres Sexuais
[Mh] Termos MeSH secundário: Índice de Massa Corporal
Desoxirribonuclease HindIII/metabolismo
Grupos Étnicos/genética
Feminino
Frequência do Gene/genética
Genótipo
Glucose/metabolismo
Saúde
Seres Humanos
Metabolismo dos Lipídeos/genética
Masculino
Adulto Jovem
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Carbohydrates); 0 (Lipids); EC 3.1.1.34 (LPL protein, human); EC 3.1.1.34 (Lipoprotein Lipase); EC 3.1.21.- (Deoxyribonuclease HindIII); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1205
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111122
[St] Status:MEDLINE


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[PMID]:21742757
[Au] Autor:Homs M; Buti M; Quer J; Jardí R; Schaper M; Tabernero D; Ortega I; Sanchez A; Esteban R; Rodriguez-Frias F
[Ad] Endereço:Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto Carlos III Corsega 180, 08036, Barcelona, Spain.
[Ti] Título:Ultra-deep pyrosequencing analysis of the hepatitis B virus preCore region and main catalytic motif of the viral polymerase in the same viral genome.
[So] Source:Nucleic Acids Res;39(19):8457-71, 2011 Oct.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hepatitis B virus (HBV) pregenomic RNA contains a hairpin structure (ε) located in the preCore region, essential for viral replication. ε stability is enhanced by the presence of preCore variants and ε is recognized by the HBV polymerase (Pol). Mutations in the retrotranscriptase domain (YMDD) of Pol are associated with treatment resistance. The aim of this study was to analyze the preCore region and YMDD motif by ultra-deep pyrosequencing (UDPS). To evaluate the UDPS error rate, an internal control sequence was inserted in the amplicon. A newly developed technique enabled simultaneous analysis of the preCore region and Pol in the same viral genome, as well as the conserved sequence of the internal control. Nucleotide errors in HindIII yielded a UDPS error rate <0.05%. UDPS study confirmed the possibility of simultaneous detection of preCore and YMDD mutations, and demonstrated the complexity of the HBV quasispecies and cooperation between viruses. Thermodynamic stability of the ε signal was found to be the main constraint for selecting main preCore mutations. Analysis of ε-signal variability suggested the essential nature of the ε structural motif and that certain nucleotides may be involved in ε signal functions.
[Mh] Termos MeSH primário: Produtos do Gene pol/genética
Genoma Viral
Vírus da Hepatite B/genética
RNA Viral/química
[Mh] Termos MeSH secundário: Adolescente
Adulto
Pareamento de Bases
Sequência de Bases
Domínio Catalítico
Códon
Análise Mutacional de DNA
Desoxirribonuclease HindIII
Produtos do Gene pol/química
Seres Humanos
Masculino
Meia-Idade
Dados de Sequência Molecular
Mutação
Conformação de Ácido Nucleico
Transativadores/genética
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Codon); 0 (Gene Products, pol); 0 (P protein, Hepatitis B virus); 0 (RNA, Viral); 0 (Trans-Activators); 0 (hepatitis B virus X protein); EC 3.1.21.- (Deoxyribonuclease HindIII)
[Em] Mês de entrada:1201
[Cu] Atualização por classe:150204
[Lr] Data última revisão:
150204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110712
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkr451


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[PMID]:21720041
[Au] Autor:Motoshima K; Ishikawa M; Hashimoto Y; Sugita K
[Ad] Endereço:Institute of Molecular & Cellular Biosciences, The University of Tokyo, Japan.
[Ti] Título:Inhibition of restriction enzymes EcoRI, BamHI and HindIII by phenethylphenylphthalimides derived from thalidomide.
[So] Source:Chem Pharm Bull (Tokyo);59(7):880-4, 2011.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:We discovered inhibitors of the restriction enzymes EcoRI, BamHI and HindIII by screening our library of compounds with a phenethylphenylphthalimide skeleton, based on α-glucosidase inhibitors and liver X receptor antagonists derived from thalidomide. Structural development afforded the potent restriction enzyme inhibitors 25 and 26.
[Mh] Termos MeSH primário: Desoxirribonuclease BamHI/antagonistas & inibidores
Desoxirribonuclease EcoRI/antagonistas & inibidores
Desoxirribonuclease HindIII/antagonistas & inibidores
Inibidores Enzimáticos/química
Isoindóis/química
Ftalimidas/química
Talidomida/química
[Mh] Termos MeSH secundário: Desoxirribonuclease BamHI/metabolismo
Desoxirribonuclease EcoRI/metabolismo
Desoxirribonuclease HindIII/metabolismo
Inibidores Enzimáticos/síntese química
Inibidores Enzimáticos/farmacologia
Inibidores de Glicosídeo Hidrolases
Isoindóis/síntese química
Isoindóis/farmacologia
Receptores X do Fígado
Receptores Nucleares Órfãos/antagonistas & inibidores
Receptores Nucleares Órfãos/metabolismo
Ftalimidas/síntese química
Ftalimidas/farmacologia
alfa-Glucosidases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (4,5,6,7-tetrachloro-2-(3-(2-(3,4,5-trihydroxyphenyl)ethyl)phenyl)isoindole-1,3-dione); 0 (4,5,6,7-tetrachloro-2-(4-(2-(3,4,5-trihydroxyphenyl)ethyl)phenyl)isoindole-1,3-dione); 0 (Enzyme Inhibitors); 0 (Glycoside Hydrolase Inhibitors); 0 (Isoindoles); 0 (Liver X Receptors); 0 (Orphan Nuclear Receptors); 0 (Phthalimides); 4Z8R6ORS6L (Thalidomide); EC 3.1.21.- (Deoxyribonuclease BamHI); EC 3.1.21.- (Deoxyribonuclease EcoRI); EC 3.1.21.- (Deoxyribonuclease HindIII); EC 3.2.1.20 (alpha-Glucosidases)
[Em] Mês de entrada:1110
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110702
[St] Status:MEDLINE


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[PMID]:21718073
[Au] Autor:An H; Jin B
[Ad] Endereço:School of Earth and Environmental Sciences, The University of Adelaide, Adelaide, SA, Australia.
[Ti] Título:DNA exposure to buckminsterfullerene (C60): toward DNA stability, reactivity, and replication.
[So] Source:Environ Sci Technol;45(15):6608-16, 2011 Aug 01.
[Is] ISSN:1520-5851
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Buckminsterfullerene (C(60)) has received great research interest due to its extraordinary properties and increasing applications in manufacturing industry and biomedical technology. We recently reported C(60) could enter bacterial cells and bind to DNA molecules. This study was to further determine how the DNA-C(60) binding affected the thermal stability and enzymatic digestion of DNA molecules, and DNA mutations. Nano-C(60) aggregates and water-soluble fullerenols were synthesized and their impact on DNA biochemical and microbial activity was investigated. Our results revealed that water-soluble fullerenols could bind to lambda DNA and improve DNA stability remarkably against thermal degradation at 70-85 °C in a dose-dependent manner. DNase I and HindIII restriction endonuclease activities were inhibited after interacting with fullerenols at a high dose. Experimental results also showed the different influence of fullerenol and nano-C(60) on their antibacterial mechanisms, where fullerenols contributed considerable impact on cell damage and mutation rate. This preliminary study indicated that the application of fullerenols results in significant changes in the physical structures and biochemical functions of DNA molecules.
[Mh] Termos MeSH primário: Replicação do DNA
DNA/metabolismo
Fulerenos/química
[Mh] Termos MeSH secundário: Fenômenos Químicos/efeitos dos fármacos
DNA/química
Replicação do DNA/efeitos dos fármacos
Desoxirribonuclease HindIII/metabolismo
Desoxirribonuclease I/metabolismo
Escherichia coli/citologia
Escherichia coli/efeitos dos fármacos
Escherichia coli/metabolismo
Fulerenos/toxicidade
Viabilidade Microbiana/efeitos dos fármacos
Mutação/genética
Nanopartículas/ultraestrutura
Conformação de Ácido Nucleico
Tamanho da Partícula
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fullerenes); 182024-42-6 (fullerenol); 9007-49-2 (DNA); EC 3.1.21.- (Deoxyribonuclease HindIII); EC 3.1.21.1 (Deoxyribonuclease I)
[Em] Mês de entrada:1111
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110702
[St] Status:MEDLINE
[do] DOI:10.1021/es2012319


  7 / 1195 MEDLINE  
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[PMID]:20364832
[Au] Autor:Liu KJ; Brock MV; Shih IeM; Wang TH
[Ad] Endereço:Biomedical Engineering Department, 3400 North Charles Street, Johns Hopkins University, Baltimore, Maryland 21218, USA.
[Ti] Título:Decoding circulating nucleic acids in human serum using microfluidic single molecule spectroscopy.
[So] Source:J Am Chem Soc;132(16):5793-8, 2010 Apr 28.
[Is] ISSN:1520-5126
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Circulating nucleic acid (CNA) has been the focus of recent research as a noninvasive source of biomarker candidates. Among these markers, DNA fragment size has shown promise for discerning the source of CNA molecules in cancer and prenatal diagnostics. We have developed a one-step assay for analyzing circulating DNA size and quantity directly in human serum. Microfluidic cylindrical illumination confocal spectroscopy and fluorescence burst size analysis are used to individually count and size fluorescently-labeled CNA molecules as they are driven through a microfluidic constriction. First, single molecule sizing was performed on lambda Hind III digest DNA to obtain a size calibration curve. A linear relation between DNA length and burst size was seen from 564 bp to 27.5 kbp. Subsequently, the single molecule assay parameters were optimized. Finally, DNA sizing analysis was performed on serum samples from both early and late stage lung cancer patients. This assay was performed directly in patient serum using only a single reagent, a simple DNA intercalating dye, and without the need for DNA isolation or enzymatic amplification steps. This demonstrates that microfluidic single molecule spectroscopy can be a rapid, facile, and inexpensive alternative to the established PCR-based methods that have been used near exclusively for CNA analysis.
[Mh] Termos MeSH primário: DNA/sangue
Técnicas Analíticas Microfluídicas
Análise Espectral/instrumentação
[Mh] Termos MeSH secundário: Calibragem
DNA/química
DNA/isolamento & purificação
DNA/metabolismo
Desoxirribonuclease HindIII/metabolismo
Seres Humanos
Indicadores e Reagentes
Lasers
Neoplasias Pulmonares/sangue
Neoplasias Pulmonares/patologia
Estadiamento de Neoplasias
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Indicators and Reagents); 9007-49-2 (DNA); EC 3.1.21.- (Deoxyribonuclease HindIII)
[Em] Mês de entrada:1008
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100407
[St] Status:MEDLINE
[do] DOI:10.1021/ja100342q


  8 / 1195 MEDLINE  
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[PMID]:20092038
[Au] Autor:Araújo LM; Cendoroglo MS; Gigek CO; Chen ES; Smith Mde A
[Ad] Endereço:Disciplina de Geriatria e Gerontologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil. lara.mqaraujo@gmail.com
[Ti] Título:Association of lipase lipoprotein polymorphisms with high-density lipoprotein and triglycerides in elderly men.
[So] Source:Genet Mol Res;9(1):86-96, 2010.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Lipoprotein lipase is essential for triglyceride hydrolysis. The polymorphisms S447X in exon 9 and HindIII in intron 8 have been associated with lower triglyceride levels and lower cardiovascular risk in adult men. We examined the association of these lipoprotein lipase polymorphisms with high-density lipoprotein (HDL) and triglyceride levels in elderly men. Blood samples were obtained from 87 elderly men, 48 of whom had cardiovascular disease and 39 (controls) had no history of cardiovascular events. The lipoprotein lipase polymorphisms were analyzed by PCR-RFLP. Allele frequencies were H- = 27.9% and X = 21.5%. There were no significant differences in allele frequencies or blood lipid levels between cardiovascular disease and control groups. However, the X allele was associated with a lower triglyceride/HDL ratio, 2.30 vs 3.02 for X allele absent (P = 0.03); the H-X haplotype was associated with lower triglyceride levels compared to the H+S haplotype (1.22 vs 1.58 mM, respectively) and a lower triglyceride/HDL ratio (2.29 vs 3.26, respectively). The X allele and H-X haplotype were associated with lower triglyceride/HDL ratios in these elderly men, independent of the history of cardiovascular events.
[Mh] Termos MeSH primário: Lipase Lipoproteica/genética
Lipoproteínas HDL/sangue
Triglicerídeos/sangue
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Brasil
Desoxirribonuclease HindIII/química
Éxons
Frequência do Gene
Genes Ligados ao Cromossomo X
Haplótipos
Seres Humanos
Íntrons
Masculino
Polimorfismo Genético
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lipoproteins, HDL); 0 (Triglycerides); EC 3.1.1.34 (Lipoprotein Lipase); EC 3.1.21.- (Deoxyribonuclease HindIII)
[Em] Mês de entrada:1003
[Cu] Atualização por classe:100121
[Lr] Data última revisão:
100121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100123
[St] Status:MEDLINE


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[PMID]:20228579
[Au] Autor:Li S; Ma Y; Jang S; Wu Y; Liu H; Cao Z; Li W
[Ad] Endereço:State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, People's Republic of China.
[Ti] Título:A HindIII BAC library construction of Mesobuthus martensii Karsch (Scorpiones:Buthidae): an important genetic resource for comparative genomics and phylogenetic analysis.
[So] Source:Genes Genet Syst;84(6):417-24, 2009 Dec.
[Is] ISSN:1341-7568
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Scorpions are "living but sophisticated fossils" that have changed little in their morphology since their first appearance over the past 450 million years ago. To provide a genetic resource for understanding the evolution of scorpion genome and the relationships between scorpions and other organisms, we first determined the genome size of the scorpion Mesobuthus martensii Karsch (about 600 Mbp) in the order Scorpiones and constructed a HindIII BAC library of the male scorpion M. martensii Karsch from China. The BAC library consists of a total of 46,080 clones with an average insert size of 100 kb, providing a 7.7-fold coverage of the scorpion haploid genome size of 600 Mbp as revealed in this study. High-density colony hybridization-based library screening was performed using 18S-5.8S-28S rRNA gene that is one of the most commonly used phylogenetic markers. Both library screening and PCR identification results revealed six positive BAC clones which were overlapped, and formed a contig of approximately 120 kb covering the rDNA. BAC DNA sequencing analysis determined the complete sequence of M. martensii Karsch rDNA unit that has a total length of 8779 bp, including 1813 bp 18s rDNA, 157 bp 5.8s rDNA, 3823 bp 28s rDNA, 530 bp ETS, 2168 bp ITS1 and 288 bp ITS2. Interestingly, some tandem repeats are present in the rRNA intergenic sequence (IGS) and ITS1/2 regions. These results demonstrated that the BAC library of the scorpion M. martensii Karsch and the complete sequence of rDNA unit will provide important genetic resources and tools for comparative genomics and phylogenetic analysis.
[Mh] Termos MeSH primário: Cromossomos Artificiais Bacterianos/genética
Desoxirribonuclease HindIII/metabolismo
Biblioteca Genômica
Escorpiões/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
DNA Ribossômico/química
DNA Ribossômico/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Evolução Molecular
Masculino
Dados de Sequência Molecular
Filogenia
Proteômica/métodos
RNA Ribossômico 18S/genética
RNA Ribossômico 23S/genética
RNA Ribossômico 5,8S/genética
Escorpiões/classificação
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 18S); 0 (RNA, Ribosomal, 23S); 0 (RNA, Ribosomal, 5.8S); EC 3.1.21.- (Deoxyribonuclease HindIII)
[Em] Mês de entrada:1005
[Cu] Atualização por classe:100315
[Lr] Data última revisão:
100315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100316
[St] Status:MEDLINE


  10 / 1195 MEDLINE  
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[PMID]:20147067
[Au] Autor:Cope NF; Fraser P
[Ad] Endereço:Laboratory of Chromatin and Gene Expression, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, United Kingdom. nathan.cope@bbsrc.ac.uk
[Ti] Título:Chromosome conformation capture.
[So] Source:Cold Spring Harb Protoc;2009(2):pdb.prot5137, 2009 Feb.
[Is] ISSN:1559-6095
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Cromossomos de Mamíferos/genética
DNA/genética
Conformação de Ácido Nucleico
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Animais
DNA/química
DNA/metabolismo
Desoxirribonuclease HindIII/metabolismo
Formaldeído
Camundongos
Modelos Moleculares
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
1HG84L3525 (Formaldehyde); 9007-49-2 (DNA); EC 3.1.21.- (Deoxyribonuclease HindIII)
[Em] Mês de entrada:1006
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100212
[St] Status:MEDLINE
[do] DOI:10.1101/pdb.prot5137



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