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Pesquisa : D08.811.277.040.025.095 [Categoria DeCS]
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[PMID]:29227609
[Au] Autor:Nozdrenko DM; Miroshnychenko MS; Soroca VM; Korchins ka LV; Zavodovskiy DO
[Ti] Título:The effect of chlorpyrifos upon ATPase activity of sarcoplasmic reticulum and biomechanics of skeleta l muscle contraction.
[So] Source:Ukr Biochem J;88(2):82-8, 2016 Mar-Apr.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:We investigated the effect of chlorpyrifos, an organophosphate insecticide, on Ca2+,Mg2+-ATPase activity of sarcoplasmic reticulum and on contraction dynamics (force and length changes) of Rana temporaria m. tibialis anterior muscle fiber bundles. All of the used concentrations of chlorpyrifos (10-6 to 10-5 M) caused decrease of Ca2+,Mg2+-ATPase activity. The inhibition of Ca2+,Mg2+-ATPase activity by chlorpyriphos in concentrations of 10-6 M to 7.5·10-6 M is due to permeation of sarcoplasmic reticulum rather than due to direct enzyme inhibition by organophosphate insecticides. The inhibitory properties of the compound were higher at increased concentration and exposure timeframes. Chlorpyrifos in concentration range of 10-6 to 7.5·10-6 M causes changes in muscle fiber response force that were more pronounced than changes in contractile length. We demonstrated inhibition of Ca2+,Mg2+-ATPase activity caused by noncholinergic effects of chlorpyriphos. It is possible to conclude that influence of organophosphate insecticides happens not only in the neuromuscular transmission but also on the level of subcellular structures.
[Mh] Termos MeSH primário: ATPase de Ca(2+) e Mg(2+)/antagonistas & inibidores
Clorpirifos/farmacologia
Inseticidas/farmacologia
Contração Muscular/efeitos dos fármacos
Fibras Musculares Esqueléticas/efeitos dos fármacos
Retículo Sarcoplasmático/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
ATPase de Ca(2+) e Mg(2+)/metabolismo
Cálcio/metabolismo
Relação Dose-Resposta a Droga
Transporte de Íons/efeitos dos fármacos
Contração Muscular/fisiologia
Fibras Musculares Esqueléticas/fisiologia
Rana temporaria
Retículo Sarcoplasmático/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); EC 3.6.1.- (Ca(2+) Mg(2+)-ATPase); JCS58I644W (Chlorpyrifos); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.02.082


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[PMID]:29227596
[Au] Autor:Veklich TO
[Ti] Título:The inhibitory influence of calix[4]Arene of C-90 on the activity of Ca2+,Mg2+-ATPases in plasma membrane and sarcoplasmic reticulum in myometrium cells.
[So] Source:Ukr Biochem J;88(2):5-15, 2016 Mar-Apr.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Our study on the plasma membrane vesicles and myometrium cells treated with 0.1% digitonin showed that inhibitory effect of calix[4]arene C-90 (5,11,17,23-tetra(trifluoro)methyl(phenylsulphonylimino)-methylamino- 25,26,27,28-tetrapropoxy-calix[4]arene) on the plasma membrane Ca2+,Mg2+-ATPase was more significant than on the Ca2+,Mg2+-ATPase in sarcoplasmic reticulum (the inhibition coefficient I0.5 values were 20.2 ± 0.5 µM and 57.0 ± 1.4 µM for the plasma membrane Ca2+,Mg2+-ATPase and Ca2+,Mg2+-ATPase in sarcoplasmic reticulum, respectively (n = 5)). Inhibition kinetics of calix[4]arene C-90 effect on the Ca2+,Mg2+- ATPase activities in plasma membrane and sarcoplasmic reticulum were studied. This substance inhibited both pumps as complete noncompetitive inhibitor. Calix[4]arene C-90 caused the increase of intracellular Ca2+ concentration and decrease of hydrodynamic diameter in smooth muscle cells similar to the action of uterotonic drug oxytocin.
[Mh] Termos MeSH primário: ATPase de Ca(2+) e Mg(2+)/antagonistas & inibidores
Calixarenos/farmacologia
Membrana Celular/efeitos dos fármacos
Inibidores Enzimáticos/farmacologia
Miócitos de Músculo Liso/efeitos dos fármacos
Fenóis/farmacologia
Retículo Sarcoplasmático/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
ATPase de Ca(2+) e Mg(2+)/metabolismo
Cálcio/metabolismo
Membrana Celular/enzimologia
Tamanho Celular
Feminino
Transporte de Íons/efeitos dos fármacos
Cinética
Miócitos de Músculo Liso/enzimologia
Miométrio/efeitos dos fármacos
Miométrio/enzimologia
Ocitocina/farmacologia
Ligação Proteica
Retículo Sarcoplasmático/enzimologia
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Phenols); 0 (calix(4)arene); 130036-26-9 (Calixarenes); 50-56-6 (Oxytocin); EC 3.6.1.- (Ca(2+) Mg(2+)-ATPase); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.02.005


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[PMID]:28945775
[Au] Autor:Chen J; Huang M; Cao F; Pardha-Saradhi P; Zou Y
[Ad] Endereço:Southern Regional Collaborative Innovation Center for Grain and Oil Crops (CICGO), Hunan Agricultural University, Changsha, China.
[Ti] Título:Urea application promotes amino acid metabolism and membrane lipid peroxidation in Azolla.
[So] Source:PLoS One;12(9):e0185230, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A pot experiment was conducted to evaluate the effect of urea on nitrogen metabolism and membrane lipid peroxidation in Azolla pinnata. Compared to controls, the application of urea to A. pinnata resulted in a 44% decrease in nitrogenase activity, no significant change in glutamine synthetase activity, 660% higher glutamic-pyruvic transaminase, 39% increase in free amino acid levels, 22% increase in malondialdehyde levels, 21% increase in Na+/K+- levels, 16% increase in Ca2+/Mg2+-ATPase levels, and 11% decrease in superoxide dismutase activity. In terms of H2O2 detoxifying enzymes, peroxidase activity did not change and catalase activity increased by 64% in urea-treated A. pinnata. These findings suggest that urea application promotes amino acid metabolism and membrane lipid peroxidation in A. pinnata.
[Mh] Termos MeSH primário: Gleiquênias/efeitos dos fármacos
Gleiquênias/metabolismo
Peroxidação de Lipídeos/efeitos dos fármacos
Ureia/farmacologia
[Mh] Termos MeSH secundário: Aminoácidos/metabolismo
ATPase de Ca(2+) e Mg(2+)/metabolismo
Catalase/metabolismo
Gleiquênias/crescimento & desenvolvimento
Peróxido de Hidrogênio/metabolismo
Malondialdeído/metabolismo
Lipídeos de Membrana/metabolismo
Nitrogênio/metabolismo
ATPase Trocadora de Sódio-Potássio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Membrane Lipids); 4Y8F71G49Q (Malondialdehyde); 8W8T17847W (Urea); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.6 (Catalase); EC 3.6.1.- (Ca(2+) Mg(2+)-ATPase); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase); N762921K75 (Nitrogen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185230


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[PMID]:28648599
[Au] Autor:Komatsu H; Koseki Y; Kanno T; Aoki S; Kodama T
[Ad] Endereço:Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Kawazu 680-4, Iizuka 820-8502, Japan. Electronic address: komatsu@bio.kyutech.ac.jp.
[Ti] Título:2,3-Butandione 2-monoxime inhibits skeletal myosin II by accelerating ATP cleavage.
[So] Source:Biochem Biophys Res Commun;490(3):849-854, 2017 Aug 26.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:2,3-Butandione 2-monoxime (BDM) is a widely used myosin inhibitor with an unclear mode of action. In this report, we investigated the mechanism of BDM oxime group nucleophilic reactivity on the phosphoester bond of ATP. BDM increased the ATPase activity of skeletal myosin subfragment 1 (S1) under conditions in which ATP cleavage is the rate-limiting step (K , EDTA-ATPase activity of native S1 and Mg -ATPase activity of trinitrophenylated S1 and partially unfolded S1). Furthermore, the effect of BDM on the S1-bound adenosine 5'-(ß,γ-imido) triphosphate (AMPPNP) P NMR spectrum suggests that BDM changes the microenvironment around the phosphorus atoms of myosin-bound nucleotide. A computational search for the BDM-binding site in the adenosine 5'-[γ-thio] triphosphate (myosin-ATPγS) complex predicted that BDM is located adjacent to the nucleotide on myosin. Therefore, we propose that the BDM oxime group catalytically assists in ATP cleavage, thereby enhancing the ATPase activity of myosin in a manner analogous to pralidoxime-mediated reactivation of organophosphate-inactivated acetylcholinesterase. This is the first study suggesting that oxime provides catalytic assistance for ATP cleavage by an ATP-hydrolyzing enzyme.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/metabolismo
Miosina Tipo II/antagonistas & inibidores
Miosina Tipo II/metabolismo
Oximas/química
Oximas/farmacologia
[Mh] Termos MeSH secundário: Adenosina Trifosfatases/metabolismo
Animais
ATPase de Ca(2+) e Mg(2+)/metabolismo
Simulação de Acoplamento Molecular
Subfragmentos de Miosina/metabolismo
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Myosin Subfragments); 0 (Oximes); 8L70Q75FXE (Adenosine Triphosphate); EC 3.6.1.- (Adenosine Triphosphatases); EC 3.6.1.- (Ca(2+) Mg(2+)-ATPase); EC 3.6.1.- (EDTA-ATPase); EC 3.6.1.- (Myosin Type II)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE


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[PMID]:28276710
[Au] Autor:Ben Saad H; Kharrat N; Driss D; Gargouri M; Marrakchi R; Jammoussi K; Magné C; Boudawara T; Ellouz Chaabouni S; Zeghal KM; Hakim A; Ben Amara I
[Ad] Endereço:a Faculty of Medicine , Laboratory of Pharmacology, University of Sfax , Tunisia.
[Ti] Título:Effects of vanillin on potassium bromate-induced neurotoxicity in adult mice: impact on behavior, oxidative stress, genes expression, inflammation and fatty acid composition.
[So] Source:Arch Physiol Biochem;123(3):165-174, 2017 Jul.
[Is] ISSN:1744-4160
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Vanillin is known to possess important antioxidant activity. OBJECTIVE: The current study was conducted to establish the therapeutic efficiency of vanillin against potassium bromate (KBrO )-induced depression-like behavior and oxidative stress in mice. MATERIAL AND METHODS: Mice were exposed during 15 days either to potassium bromate (KBrO ), KBrO + vanillin or to only vanillin. RESULTS: Our results revealed a significant modification in the fatty acid composition of the KBrO -treated mice. In addition, KBrO induced a significant reduction in enzymatic activities and gene expressions, Na -K and Mg -ATPases, acetylcholinesterase and butylcholinesterase activities. The gene expression of tumor necrosis factor-α, interleukin-1ß, interleukin-6 and COX , significantly increased in the cerebrum of KBrO -treated group. Histopathological observations were consistent with these effects. Co-treatment with vanillin significantly attenuated KBrO -induced oxidative stress and inflammation. CONCLUSION: This work suggests that vanillin mitigates KBrO -induced depression, and that this neuroprotective effect proceeds through anti-oxidant and anti-inflammatory activities.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Benzaldeídos/farmacologia
Depressão/prevenção & controle
Regulação da Expressão Gênica/efeitos dos fármacos
Fármacos Neuroprotetores/farmacologia
Síndromes Neurotóxicas/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Comportamento Animal/efeitos dos fármacos
Bromatos/toxicidade
Butirilcolinesterase/genética
Butirilcolinesterase/metabolismo
ATPase de Ca(2+) e Mg(2+)/genética
ATPase de Ca(2+) e Mg(2+)/metabolismo
Cérebro/efeitos dos fármacos
Cérebro/metabolismo
Cérebro/fisiopatologia
Ciclo-Oxigenase 2/genética
Ciclo-Oxigenase 2/metabolismo
Depressão/induzido quimicamente
Depressão/genética
Depressão/metabolismo
Ácidos Graxos/metabolismo
Glutationa Peroxidase/genética
Glutationa Peroxidase/metabolismo
Interleucina-1beta/genética
Interleucina-1beta/metabolismo
Interleucina-6/genética
Interleucina-6/metabolismo
Peroxidação de Lipídeos/efeitos dos fármacos
Camundongos
Síndromes Neurotóxicas/genética
Síndromes Neurotóxicas/metabolismo
Síndromes Neurotóxicas/fisiopatologia
Estresse Oxidativo/efeitos dos fármacos
Carbonilação Proteica/efeitos dos fármacos
ATPase Trocadora de Sódio-Potássio/genética
ATPase Trocadora de Sódio-Potássio/metabolismo
Superóxido Dismutase/genética
Superóxido Dismutase/metabolismo
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Benzaldehydes); 0 (Bromates); 0 (Fatty Acids); 0 (IL1B protein, mouse); 0 (Interleukin-1beta); 0 (Interleukin-6); 0 (Neuroprotective Agents); 0 (Tumor Necrosis Factor-alpha); 0 (interleukin-6, mouse); 04MB35W6ZA (potassium bromate); CHI530446X (vanillin); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.14.99.- (Ptgs2 protein, mouse); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.15.1.1 (Superoxide Dismutase); EC 3.1.1.8 (Butyrylcholinesterase); EC 3.6.1.- (Ca(2+) Mg(2+)-ATPase); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1080/13813455.2017.1283527


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[PMID]:27878299
[Au] Autor:Yu L; Yang J; Wang X; Jiang B; Sun Y; Ji Y
[Ad] Endereço:Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, P.R. China.
[Ti] Título:Antioxidant and antitumor activities of Capparis spinosa L. and the related mechanisms.
[So] Source:Oncol Rep;37(1):357-367, 2017 Jan.
[Is] ISSN:1791-2431
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:The 'ethnodrug' Capparis spinosa L. has several pharmacological activities. First, it was found in previous experiments that an ethyl acetate extract of Capparis spinosa L. (CSE) exhibited antioxidant activity. In order to further research this finding, the present study investigate the blood biochemical indices, injury, energy metabolism, oxidative damage and mitochondrial membrane potential (Δψm) level of cardiac cells to study the effect of CSE on doxorubicin-induced cardiac toxicity. CSE had protective effects on the cardiac toxic effect of doxorubicin, and decreased the activity of lactic dehydrogenase (LDH) and creatine kinase (CK). CSE increased the ability of myocardial tissue to scavenge free radicals, inhibited lipid peroxidation, increased recovery activity of antioxidant enzymes, adjusted the energy metabolism of myocardial tissue, inhibited the generation of a large number of ROS in the cells, raised the level of Δψm, and improved the metabolism of free radicals. CSE demonstrated protective effects on doxorubicin-induced myocardial damage. Second, the quaternary ammonium hydroxide of Capparis spinosa L. (CSQAH) was found to possess antitumor activity, such as antiproliferative and apoptosis-induced effects on HepG2 cells. We investigated the regulatory mechanism of HepG2 apoptosis induced by CSQAH. Laser scanning confocal microscope and Fluo-3/AM staining were utilized to detect the Ca2+ concentration in the HepG2 cells. A microplate reader was used to measure the changes in Ca2+-Mg2+-ATP enzyme. Then, flow cytometry was applied to analyze the activity of ROS and the expression levels of Bcl-2 and Bax. As a result, different concentrations of CSQAH increased the concentration of Ca2+ in the cytoplasm in a dosage-dependent manner. CSQAH decreased the Ca2+­Mg2+­ATPase activity in the HepG2 cells. The levels of ROS in the CSQAH groups were significantly higher than the level in the control group. Flow cytometric analysis showed that the Bcl-2 expression levels in the CSQAH-treated groups were downregulated, while Bax expression levels were upregulated, and the effects were dosage-dependent. The regulatory mechanism of HepG2 cell apoptosis induced by CSQAH involved the increase in Ca2+ concentration and ROS levels, a decrease in Ca2+-Mg2+-ATPase activity in the HepG2 cells, and downregulation of anti-apoptotic Bcl-2 expression, and upregulation of apoptotic Bax expression. In summary, the present study demonstrated the antioxidant and antitumor activities of CSE which may suppress tumor growth and alleviate the side-effects of DOX, which may facilitate tumor treatment in a dual manner.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Antioxidantes/farmacologia
Capparis/química
Doxorrubicina/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
ATPase de Ca(2+) e Mg(2+)/metabolismo
Avaliação Pré-Clínica de Medicamentos/métodos
Feminino
Células Hep G2/efeitos dos fármacos
Seres Humanos
Masculino
Camundongos
Miócitos Cardíacos/efeitos dos fármacos
Miócitos Cardíacos/metabolismo
Miócitos Cardíacos/patologia
Substâncias Protetoras/farmacologia
Espécies Reativas de Oxigênio/metabolismo
Proteína X Associada a bcl-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (BAX protein, human); 0 (Protective Agents); 0 (Reactive Oxygen Species); 0 (bcl-2-Associated X Protein); 80168379AG (Doxorubicin); EC 3.6.1.- (Ca(2+) Mg(2+)-ATPase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170322
[Lr] Data última revisão:
170322
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE
[do] DOI:10.3892/or.2016.5249


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[PMID]:27706697
[Au] Autor:Zheng Y; Qiu L; Fan L; Song C; Meng S; Chen J
[Ad] Endereço:Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, China zhengy@ffrc.cn.
[Ti] Título:Effect of polychlorinated biphenyls on osmoregulatory response and apoptosis in GIFT tilapia, Oreochromis niloticus.
[So] Source:Genet Mol Res;15(3), 2016 Sep 02.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:In the present study, GIFT tilapia Oreochromis niloticus were exposed to polychlorinated biphenyls (PCBs) for 7, 14, and 21 days. Over the duration of the exposure, genotoxicity and the activity of Na /K -ATPase (NKA) and Ca /Mg -ATPase (CMA) were measured in the gill, kidney, and intestine, to evaluate changes in osmoregulatory response in O. niloticus. Our results showed significant decreases in organic NKA (except in gill tissues after 0.5 mg/L PCB-exposure) and CMA activity. The results of the genotoxicity assay showed significant increases in atp1a1a, nkcc2 (only in gill tissue), and fxyd7 (except after 21 days of 5 mg/L PCB exposure). We found significant increases in caspase proteins in the liver in the 5-mg/L PCB exposure group, and the transcripts showed dose-dependent increases between treatment groups over the exposure duration. This study presents evidence that chronic exposure to PCB could result in organic osmoregulatory response and hepatic apoptosis in GIFT tilapia.
[Mh] Termos MeSH primário: Proteínas de Peixes/genética
Fígado/efeitos dos fármacos
Osmorregulação/efeitos dos fármacos
Bifenilos Policlorados/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
ATPase de Ca(2+) e Mg(2+)/genética
ATPase de Ca(2+) e Mg(2+)/metabolismo
Ciclídeos
Proteínas de Peixes/metabolismo
Regulação da Expressão Gênica
Brânquias/efeitos dos fármacos
Brânquias/metabolismo
Glutationa Peroxidase/genética
Glutationa Peroxidase/metabolismo
Intestinos/efeitos dos fármacos
Intestinos/metabolismo
Rim/efeitos dos fármacos
Rim/metabolismo
Fígado/crescimento & desenvolvimento
Fígado/metabolismo
Fígado/patologia
Glicoproteínas de Membrana/genética
Glicoproteínas de Membrana/metabolismo
ATPase Trocadora de Sódio-Potássio/genética
ATPase Trocadora de Sódio-Potássio/metabolismo
Membro 1 da Família 12 de Carreador de Soluto/genética
Membro 1 da Família 12 de Carreador de Soluto/metabolismo
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Membrane Glycoproteins); 0 (Solute Carrier Family 12, Member 1); 0 (Water Pollutants, Chemical); DFC2HB4I0K (Polychlorinated Biphenyls); EC 1.11.1.9 (Glutathione Peroxidase); EC 3.6.1.- (Ca(2+) Mg(2+)-ATPase); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161006
[St] Status:MEDLINE
[do] DOI:10.4238/gmr.15038620


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[PMID]:27558241
[Au] Autor:Kirakosyan G; Mohamadvarzi M; Ghulikyan L; Zaqaryan N; Kishmiryan A; Ayvazyan N
[Ad] Endereço:Orbeli Institute of Physiology, National Academy of Sciences of the Republic of Armenia, Orbely str. 22, 0019 Yerevan, Armenia.
[Ti] Título:Morphological and functional alteration of erythrocyte ghosts and giant unilamellar vesicles caused by Vipera latifi venom.
[So] Source:Comp Biochem Physiol C Toxicol Pharmacol;190:48-53, 2016 Dec.
[Is] ISSN:1532-0456
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Snake bites are an endemic public health problem in Iran, both in rural and urban area. Viper venom as a hemolytic biochemical "cocktail" of toxins, primarily cause to the systemic alteration of blood cells. In the sixties and seventies, human erythrocytes were extensively studied, but the mechanical and chemical stresses commonly exerted on red blood cells continue to attract interest of scientists for the study of membrane structure and function. Here, we monitor the effect of Vipera latifi venom on human erythrocytes ghost membranes using phase contrast and fluorescent microscopy and changes in ATPase activity under snake venom influence in vitro. The ion pumps [Na ,K ]-ATPase and (Ca +Mg )-ATPase plays a pivotal role in the active transport of certain cations and maintenance of intracellular electrolyte homeostasis. We also describe the interaction of Vipera latifi (VL) venom with giant unilamellar vesicles (GUVs) composed of the native phospholipid mixtures visualized by the membrane fluorescence probe, ANS, used to assess the state of membrane and specifically mark the phospholipid domains.
[Mh] Termos MeSH primário: ATPase de Ca(2+) e Mg(2+)/metabolismo
Membrana Eritrocítica/efeitos dos fármacos
Fosfolipídeos/metabolismo
ATPase Trocadora de Sódio-Potássio/metabolismo
Lipossomas Unilamelares/metabolismo
Venenos de Víboras/toxicidade
[Mh] Termos MeSH secundário: Membrana Eritrocítica/enzimologia
Membrana Eritrocítica/patologia
Seres Humanos
Cinética
Microscopia de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phospholipids); 0 (Unilamellar Liposomes); 0 (Viper Venoms); EC 3.6.1.- (Ca(2+) Mg(2+)-ATPase); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160826
[St] Status:MEDLINE


  9 / 3370 MEDLINE  
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[PMID]:27170289
[Au] Autor:Meng T; Bu W; Ren X; Chen X; Yu J; Eckenhoff RG; Gao WD
[Ad] Endereço:Department of Anesthesiology, Qilu Hospital of Shandong University, Jinan, Shandong, China;
[Ti] Título:Molecular mechanism of anesthetic-induced depression of myocardial contraction.
[So] Source:FASEB J;30(8):2915-25, 2016 08.
[Is] ISSN:1530-6860
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Isoflurane and propofol are known to depress cardiac contraction, but the molecular mechanisms involved are not known. In this study, we determined whether decreasing myofilament Ca(2+) responsiveness underlies anesthesia-induced depression of contraction and uncovered the molecular targets of isoflurane and propofol. Force and intracellular Ca(2+) ([Ca(2+)]i) were measured in rat trabeculae superfused with Krebs-Henseleit solution, with or without propofol or isoflurane. Photoaffinity labeling of myofilament proteins with meta-Azi-propofol (AziPm) and Azi-isoflurane (Azi-iso) and molecular docking were also used. Both propofol and isoflurane dose dependently depressed force from low doses (propofol, 27 ± 6 µM; isoflurane, 1.0 ± 0.1%) to moderate doses (propofol, 87 ± 4 µM; isoflurane, 3.0 ± 0.25%), without significant alteration [Ca(2+)]i During steady-state activations in both intact and skinned preparations, propofol and isoflurane depressed maximum Ca(2+)-activated force and increased the [Ca(2+)]i required for 50% of activation. Myofibrils photolabeled with AziPm and Azi-iso identified myosin, actin, and myosin light chain as targets of the anesthetics. Several adducted residues in those proteins were located in conformationally sensitive regions that underlie contractile function. Thus, propofol and isoflurane decrease force development by directly depressing myofilament Ca(2+) responsiveness and have binding sites in key regions for contraction in both actin and myosin.-Meng, T., Bu, W., Ren, X., Chen, X., Yu, J., Eckenhoff, R. G., Gao, W. D. Molecular mechanism of anesthetic-induced depression of myocardial contraction.
[Mh] Termos MeSH primário: Anestésicos Inalatórios/farmacologia
Hipnóticos e Sedativos/farmacologia
Isoflurano/farmacologia
Contração Miocárdica/efeitos dos fármacos
Miofibrilas/efeitos dos fármacos
Propofol/farmacologia
[Mh] Termos MeSH secundário: Anestésicos Inalatórios/química
ATPase de Ca(2+) e Mg(2+)/metabolismo
Cálcio/metabolismo
Cálcio/farmacologia
Corantes
Seres Humanos
Hipnóticos e Sedativos/química
Isoflurano/química
Modelos Moleculares
Miosinas/química
Miosinas/fisiologia
Estresse Oxidativo/efeitos dos fármacos
Estresse Oxidativo/fisiologia
Propofol/química
Ligação Proteica
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anesthetics, Inhalation); 0 (Coloring Agents); 0 (Hypnotics and Sedatives); CYS9AKD70P (Isoflurane); EC 3.6.1.- (Ca(2+) Mg(2+)-ATPase); EC 3.6.4.1 (Myosins); SY7Q814VUP (Calcium); YI7VU623SF (Propofol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160513
[St] Status:MEDLINE
[do] DOI:10.1096/fj.201600290RR


  10 / 3370 MEDLINE  
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[PMID]:27063002
[Au] Autor:Peng C; Zhao X; Liu S; Shi W; Han Y; Guo C; Jiang J; Wan H; Shen T; Liu G
[Ad] Endereço:College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China.
[Ti] Título:Effects of anthropogenic sound on digging behavior, metabolism, Ca(2+)/Mg(2+) ATPase activity, and metabolism-related gene expression of the bivalve Sinonovacula constricta.
[So] Source:Sci Rep;6:24266, 2016 Apr 11.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Anthropogenic sound has increased significantly in the past decade. However, only a few studies to date have investigated its effects on marine bivalves, with little known about the underlying physiological and molecular mechanisms. In the present study, the effects of different types, frequencies, and intensities of anthropogenic sounds on the digging behavior of razor clams (Sinonovacula constricta) were investigated. The results showed that variations in sound intensity induced deeper digging. Furthermore, anthropogenic sound exposure led to an alteration in the O:N ratios and the expression of ten metabolism-related genes from the glycolysis, fatty acid biosynthesis, tryptophan metabolism, and Tricarboxylic Acid Cycle (TCA cycle) pathways. Expression of all genes under investigation was induced upon exposure to anthropogenic sound at ~80 dB re 1 µPa and repressed at ~100 dB re 1 µPa sound. In addition, the activity of Ca(2+)/Mg(2+)-ATPase in the feet tissues, which is directly related to muscular contraction and subsequently to digging behavior, was also found to be affected by anthropogenic sound intensity. The findings suggest that sound may be perceived by bivalves as changes in the water particle motion and lead to the subsequent reactions detected in razor clams.
[Mh] Termos MeSH primário: Comportamento Animal/efeitos da radiação
Bivalves/fisiologia
ATPase de Ca(2+) e Mg(2+)/metabolismo
Som
[Mh] Termos MeSH secundário: Animais
ATPase de Ca(2+) e Mg(2+)/genética
Ciclo do Ácido Cítrico/genética
Ciclo do Ácido Cítrico/efeitos da radiação
Ácidos Graxos/biossíntese
Expressão Gênica/efeitos da radiação
Glicólise/efeitos dos fármacos
Glicólise/genética
Modelos Biológicos
Nitrogênio/química
Nitrogênio/metabolismo
Oxigênio/química
Oxigênio/metabolismo
Água do Mar/análise
Triptofano/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Acids); 8DUH1N11BX (Tryptophan); EC 3.6.1.- (Ca(2+) Mg(2+)-ATPase); N762921K75 (Nitrogen); S88TT14065 (Oxygen)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160412
[St] Status:MEDLINE
[do] DOI:10.1038/srep24266



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