Base de dados : MEDLINE
Pesquisa : D08.811.277.040.330.300.100.101 [Categoria DeCS]
Referências encontradas : 1933 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 194 ir para página                         

  1 / 1933 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29291460
[Au] Autor:Choi JH; Lee S; Nah JY; Kim HK; Paek JS; Lee S; Ham H; Hong SK; Yun SH; Lee T
[Ad] Endereço:Microbial Safety Team, National Institute of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea.
[Ti] Título:Species composition of and fumonisin production by the Fusarium fujikuroi species complex isolated from Korean cereals.
[So] Source:Int J Food Microbiol;267:62-69, 2018 Feb 21.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To assess the risk of fumonisin contamination in Korean cereals, we isolated colonies of the Fusarium fujikuroi species complex (FFSC) from barley, maize, rice and soybean samples from 2011 to 2015. A total of 878 FFSC strains were isolated mostly from maize and rice, and species identity of the isolates were determined using the DNA sequence of the translation elongation factor 1-α (TEF-1α) and RNA polymerase II (RPB2) genes. Fusaria recovered from Korean cereals included F. fujikuroi (317 isolates and a frequency of 36%), F. proliferatum (212 isolates and 24.1%), F. verticillioides (170 isolates and 19.4%), F. concentricum (86 strains and 9.8%), F. andiyazi (56 isolates and 6.4%), F. subglutinans (28 isolates and 3.2%), F. thapsinum (5 isolates and 0.6%), and F. circinatum (2 isolates and 0.2%). The rice samples were dominated by F. fujikuroi (47.4%), F. proliferatum (27.3%), and F. concentricum (15.1%), whereas maize samples were dominated by F. verticillioides (33.9%), F. fujikuroi (25.3%), and F. proliferatum (21.1%). A phylogenetic analysis of 70 representative isolates demonstrated that each species was resolved as genealogically exclusive in the ML tree. Fumonisin production potential was evaluated using a PCR assay for the fumonisin biosynthesis gene, FUM1 in all of the isolates. Most of the isolates tested (94%) were positive for FUM1. All of the isolates assigned to F. fujikuroi, F. proliferatum, F. verticillioides and F. thapsinum were positive for FUM1 irrespective of their host origin. Seventy-seven representative isolates positive for FUM1 were examined for fumonisin production in rice medium. The majority of F. proliferatum (26/27, 96.3%), F. verticillioides (16/17, 94.1%) and F. fujikuroi (19/25, 76.0%) produced both FB and FB . Notably, 16 of 19 fumonisin-producing F. fujikuroi produced >1000µg/g of fumonisins (FB +FB ) in rice medium, which is higher than that in previous reports. These results suggest that F. fujikuroi can produce high levels of fumonisins similar to F. verticillioides and F. proliferatum.
[Mh] Termos MeSH primário: Grãos Comestíveis/química
Grãos Comestíveis/microbiologia
Fumonisinas/química
Fusarium/isolamento & purificação
[Mh] Termos MeSH secundário: Biodiversidade
Fumonisinas/análise
Fumonisinas/metabolismo
Fusarium/classificação
Fusarium/genética
Fator 1 de Elongação de Peptídeos/genética
Filogenia
RNA Polimerase II/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fumonisins); 0 (Peptide Elongation Factor 1); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180102
[St] Status:MEDLINE


  2 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29342219
[Au] Autor:Hassan MK; Kumar D; Naik M; Dixit M
[Ad] Endereço:School of Biological Sciences, National Institute of Science Education and Research, HBNI, Bhimpur- Padanpur, Jatni, Khurda, Odisha, India.
[Ti] Título:The expression profile and prognostic significance of eukaryotic translation elongation factors in different cancers.
[So] Source:PLoS One;13(1):e0191377, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Eukaryotic translation factors, especially initiation factors have garnered much attention with regards to their role in the onset and progression of different cancers. However, the expression levels and prognostic significance of translation elongation factors remain poorly explored in different cancers. In this study, we have investigated the mRNA transcript levels of seven translation elongation factors in different cancer types using Oncomine and TCGA databases. Furthermore, we have identified the prognostic significance of these factors using Kaplan-Meier Plotter and SurvExpress databases. We observed altered expression levels of all the elongation factors in different cancers. Higher expression of EEF1A2, EEF1B2, EEF1G, EEF1D, EEF1E1 and EEF2 was observed in most of the cancer types, whereas reverse trend was observed for EEF1A1. Overexpression of many factors predicted poor prognosis in breast (EEF1D, EEF1E1, EEF2) and lung cancer (EEF1A2, EEF1B2, EEF1G, EEF1E1). However, we didn't see any common correlation of expression levels of elongation factors with survival outcomes across cancer types. Cancer subtype stratification showed association of survival outcomes and expression levels of elongation factors in specific sub-types of breast, lung and gastric cancer. Most interestingly, we observed a reciprocal relationship between the expression levels of the two EEF1A isoforms viz. EEF1A1 and EEF1A2, in most of the cancer types. Our results suggest that translation elongation factors can have a role in tumorigenesis and affect survival in cancer specific manner. Elongation factors have potential to serve as biomarkers and therapeutic drug targets, yet further study is required. Reciprocal relationship of differential expression between EEF1A isoforms observed in multiple cancer types indicates opposing roles in cancer and needs further investigation.
[Mh] Termos MeSH primário: Neoplasias/genética
Elongação Traducional da Cadeia Peptídica/genética
Transcriptoma/genética
[Mh] Termos MeSH secundário: Transformação Celular Neoplásica
Bases de Dados de Ácidos Nucleicos
Seres Humanos
Estimativa de Kaplan-Meier
Elongação Traducional da Cadeia Peptídica/fisiologia
Fator 1 de Elongação de Peptídeos/genética
Fator 1 de Elongação de Peptídeos/metabolismo
Prognóstico
Biossíntese de Proteínas
Isoformas de Proteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (EEF1A1 protein, human); 0 (EEF1A2 protein, human); 0 (EEF1D protein, human); 0 (Peptide Elongation Factor 1); 0 (Protein Isoforms)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191377


  3 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27773721
[Au] Autor:Tajerian M; Hung V; Khan H; Lahey LJ; Sun Y; Birklein F; Krämer HH; Robinson WH; Kingery WS; Clark JD
[Ad] Endereço:Veterans Affairs Palo Alto Health Care System Palo Alto, CA, USA; Department of Anesthesiology, Stanford University School of Medicine, Stanford, CA, USA; Palo Alto Veterans Institute for Research, Palo Alto, CA, USA. Electronic address: maral@stanford.edu.
[Ti] Título:Identification of KRT16 as a target of an autoantibody response in complex regional pain syndrome.
[So] Source:Exp Neurol;287(Pt 1):14-20, 2017 Jan.
[Is] ISSN:1090-2430
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Using a mouse model of complex regional pain syndrome (CRPS), our goal was to identify autoantigens in the skin of the affected limb. METHODS: A CRPS-like state was induced using the tibia fracture/cast immobilization model. Three weeks after fracture, hindpaw skin was homogenized, run on 2-d gels, and probed by sera from fracture and control mice. Spots of interest were analyzed by liquid chromatography-mass spectroscopy (LC-MS) and the list of targets validated by examining their abundance and subcellular localization. In order to measure the autoantigenicity of selected protein targets, we quantified the binding of IgM in control and fracture mice sera, as well as in control and CRPS human sera, to the recombinant protein. RESULTS: We show unique binding between fracture skin extracts and fracture sera, suggesting the presence of auto-antigens. LC-MS analysis provided us a list of potential targets, some of which were upregulated after fracture (KRT16, eEF1a1, and PRPH), while others showed subcellular-redistribution and increased membrane localization (ANXA2 and ENO3). No changes in protein citrullination or carbamylation were observed. In addition to increased abundance, KRT16 demonstrated autoantigenicity, since sera from both fracture mice and CRPS patients showed increased autoantibody binding to recombinant kRT16 protein. CONCLUSIONS: Pursuing autoimmune contributions to CRPS provides a novel approach to understanding the condition and may allow the development of mechanism-based therapies. The identification of autoantibodies against KRT16 as a biomarker in mice and in humans is a critical step towards these goals, and towards redefining CRPS as having an autoimmune etiology.
[Mh] Termos MeSH primário: Autoantígenos/metabolismo
Síndromes da Dor Regional Complexa/sangue
Síndromes da Dor Regional Complexa/patologia
Queratina-6/imunologia
Queratina-6/metabolismo
Pele/metabolismo
Pele/ultraestrutura
Regulação para Cima/fisiologia
[Mh] Termos MeSH secundário: Adulto
Animais
Anexina A2/metabolismo
Autoantígenos/genética
Modelos Animais de Doenças
Membro Posterior/inervação
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Meia-Idade
Fator 1 de Elongação de Peptídeos/metabolismo
Periferinas/metabolismo
Fosfopiruvato Hidratase/metabolismo
Frações Subcelulares/metabolismo
Fraturas da Tíbia/sangue
Fraturas da Tíbia/patologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ANXA2 protein, human); 0 (Annexin A2); 0 (Autoantigens); 0 (EEF1A1 protein, human); 0 (KRT6A protein, human); 0 (Keratin-6); 0 (PRPH protein, human); 0 (Peptide Elongation Factor 1); 0 (Peripherins); EC 4.2.1.11 (Phosphopyruvate Hydratase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161028
[St] Status:MEDLINE


  4 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29023479
[Au] Autor:Ebadat S; Ahmadi S; Ahmadi M; Nematpour F; Barkhordari F; Mahdian R; Davami F; Mahboudi F
[Ad] Endereço:Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
[Ti] Título:Evaluating the efficiency of CHEF and CMV promoter with IRES and Furin/2A linker sequences for monoclonal antibody expression in CHO cells.
[So] Source:PLoS One;12(10):e0185967, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In recent years, monoclonal antibodies (mAbs) have been developed as powerful therapeutic and diagnostic agents and Chinese hamster ovary (CHO) cells have emerged as the dominant host for the recombinant expression of these proteins. A critical step in recombinant expression is the utilization of strong promoters, such as the Chinese Hamster Elongation Factor-1α (CHEF-1) promoter. To compare the strengths of CHEF with cytomegalovirus (CMV) promoter for mAb expression in CHO cells, four bicistronic vectors bearing either internal ribosome entry site (IRES) or Furin/2A (F2A) sequences were designed. The efficiency of these promoters was evaluated by measuring level of expressed antibody in stable cell pools. Our results indicated that CHEF promoter-based expression of mAbs was 2.5 fold higher than CMV-based expression in F2A-mediated vectors. However, this difference was less significant in IRES-mediated mAb expressing cells. Studying the stability of the F2A expression system in the course of 18 weeks, we observed that the cells having CHEF promoter maintained their antibody expression at higher level than those transfected with CMV promoter. Further analyses showed that both IRES-mediated vectors, expressed mAbs with correct size, whereas in antibodies expressed via F2A system heterogeneity of light chains were detected due to incomplete furin cleavage. Our findings indicated that the CHEF promoter is a viable alternative to CMV promoter-based expression in F2A-mediated vectors by providing both higher expression and level of stability.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/genética
Citomegalovirus/genética
Sítios Internos de Entrada Ribossomal
Fator 1 de Elongação de Peptídeos/genética
Regiões Promotoras Genéticas
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/isolamento & purificação
Anticorpos Monoclonais/metabolismo
Western Blotting
Células CHO
Cricetulus
Furina/genética
Vetores Genéticos
Engenharia de Proteínas/métodos
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Internal Ribosome Entry Sites); 0 (Peptide Elongation Factor 1); 0 (Recombinant Proteins); EC 3.4.21.75 (Furin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185967


  5 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28985216
[Au] Autor:Hussein HE; Bastos RG; Schneider DA; Johnson WC; Adham FK; Davis WC; Laughery JM; Herndon DR; Alzan HF; Ueti MW; Suarez CE
[Ad] Endereço:Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United States of America.
[Ti] Título:The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction.
[So] Source:PLoS Negl Trop Dis;11(10):e0005965, 2017 Oct.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Babesia bovis, is a tick borne apicomplexan parasite responsible for important cattle losses globally. Babesia parasites have a complex life cycle including asexual replication in the mammalian host and sexual reproduction in the tick vector. Novel control strategies aimed at limiting transmission of the parasite are needed, but transmission blocking vaccine candidates remain undefined. Expression of HAP2 has been recognized as critical for the fertilization of parasites in the Babesia-related Plasmodium, and is a leading candidate for a transmission blocking vaccine against malaria. Hereby we identified the B. bovis hap2 gene and demonstrated that it is widely conserved and differentially transcribed during development within the tick midgut, but not by blood stage parasites. The hap2 gene was disrupted by transfecting B. bovis with a plasmid containing the flanking regions of the hap2 gene and the GPF-BSD gene under the control of the ef-1α-B promoter. Comparison of in vitro growth between a hap2-KO B. bovis clonal line and its parental wild type strain showed that HAP2 is not required for the development of B. bovis in erythrocytes. However, xanthurenic acid-in vitro induction experiments of sexual stages of parasites recovered after tick transmission resulted in surface expression of HAP2 exclusively in sexual stage induced parasites. In addition, hap2-KO parasites were not able to develop such sexual stages as defined both by morphology and by expression of the B. bovis sexual marker genes 6-Cys A and B. Together, the data strongly suggests that tick midgut stage differential expression of hap2 is associated with the development of B. bovis sexual forms. Overall these studies are consistent with a role of HAP2 in tick stages of the parasite and suggest that HAP2 is a potential candidate for a transmission blocking vaccine against bovine babesiosis.
[Mh] Termos MeSH primário: Vetores Aracnídeos/parasitologia
Babesia bovis/genética
Babesia bovis/fisiologia
Genes de Protozoários
Proteínas de Protozoários/genética
Proteínas de Protozoários/metabolismo
Rhipicephalus/parasitologia
[Mh] Termos MeSH secundário: Animais
Babesia bovis/efeitos dos fármacos
Babesia bovis/crescimento & desenvolvimento
Bovinos/parasitologia
Eritrócitos/parasitologia
Feminino
Estágios do Ciclo de Vida
Fator 1 de Elongação de Peptídeos/genética
Regiões Promotoras Genéticas
Reprodução/efeitos dos fármacos
Reprodução/genética
Xanturenatos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptide Elongation Factor 1); 0 (Protozoan Proteins); 0 (Xanthurenates); 58LAB1BG8J (xanthurenic acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005965


  6 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28895520
[Au] Autor:Kobayashi R; Kanti A; Kawasaki H
[Ad] Endereço:1​Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
[Ti] Título:Three novel species of d-xylose-assimilating yeasts, Barnettozyma xylosiphila sp. nov., Barnettozyma xylosica sp. nov. and Wickerhamomyces xylosivorus f.a., sp. nov.
[So] Source:Int J Syst Evol Microbiol;67(10):3971-3976, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study describes three novel xylose-assimilating yeasts, which were isolated from decayed wood collected from Bung Hatta Botanical Garden in West Sumatra and Cibodas Botanic Garden in West Java, or from litter from Eka Karya Bali Botanic Garden in Bali, Indonesia. Phylogenetic analysis was performed based on the sequences of the D1/D2 domains of the large ribosomal subunit (LSU), the small ribosomal subunit (SSU), the internal transcribed spacer (ITS) and elongation factor-1α (EF-1α), and the three strains were found to represent three novel species belonging to genera Barnettozyma or Wickerhamomyces. The morphological, biochemical and physiological characteristics indicated that the strains were distinct from other closely related species. Strains 13Y206 and 14Y196 belonging to the Barnettozyma clade are described as the type strains of Barnettozyma xylosiphila sp. nov. (type strain 13Y206 =NBRC 110202 =InaCC Y726 ; MycoBank MB808598) and Barnettozyma xylosica sp. nov. (type strain 14Y196 =NBRC 111558 =InaCC Y1030 ; MycoBank MB819485). Strain 14Y125 belonging to the Wickerhamomyces clade is described as the type strain of Wickerhamomyces xylosivorus f.a., sp. nov. (type strain 14Y125 =NBRC 111553 =InaCC Y1026 ; MycoBank MB819484).
[Mh] Termos MeSH primário: Filogenia
Saccharomycetales/classificação
Madeira/microbiologia
Xilose/metabolismo
[Mh] Termos MeSH secundário: DNA Fúngico/química
DNA Espaçador Ribossômico/genética
Indonésia
Técnicas de Tipagem Micológica
Fator 1 de Elongação de Peptídeos/genética
Saccharomycetales/genética
Saccharomycetales/isolamento & purificação
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal Spacer); 0 (Peptide Elongation Factor 1); A1TA934AKO (Xylose)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002233


  7 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28857022
[Au] Autor:Li WW; Zhao WX; Huai WX
[Ad] Endereço:Research Institute of Forest Ecology, Environment and Protection, The Key Laboratory of State Forestry Administration on Forest Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
[Ti] Título:Phytophthora pseudopolonica sp. nov., a new species recovered from stream water in subtropical forests of China.
[So] Source:Int J Syst Evol Microbiol;67(9):3666-3675, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new species of the genus Phytophthora was isolated from stream water in the subtropical forests of China during a survey of forest Phytophthora from 2011 to 2013. This new species is formally described here and named Phytophthora pseudopolonica sp. nov. This new homothallic species is distinct from other known Phytophthora species in morphology and produces nonpapillate and noncaducous sporangia with internal proliferation. Spherical hyphal swellings and thin-walled chlamydospores are abundant when the species is kept in sterile water. The P. pseudopolonica sp. nov. forms smooth oogonia with paragynous and sometimes amphigynous antheridia. The optimum growth temperature of the species is 30 °C in V8-juice agar with ß-sitosterol, yet it barely grows at 5 °C and 35 °C. Based on sequences of the internal transcribed spacer and the combined ß-tubulin and elongation factor 1α gene sequence data, isolates of the new species cluster together into a single branch and are close to Phytophthora polonicabelonging to clade 9.
[Mh] Termos MeSH primário: Florestas
Filogenia
Phytophthora/classificação
Rios/microbiologia
[Mh] Termos MeSH secundário: China
DNA Espaçador Ribossômico/genética
Fator 1 de Elongação de Peptídeos/genética
Phytophthora/genética
Phytophthora/isolamento & purificação
Análise de Sequência de DNA
Tubulina (Proteína)/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal Spacer); 0 (Peptide Elongation Factor 1); 0 (Tubulin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002254


  8 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28820103
[Au] Autor:Yamazaki A; Yanagiba M; Naganuma T
[Ad] Endereço:1​NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
[Ti] Título:Two novel Lipomycetaceous yeast species, Lipomyces okinawensis sp. nov. and Lipomyces yamanashiensis f.a., sp. nov., isolated from soil in the Okinawa and Yamanashi prefectures, Japan.
[So] Source:Int J Syst Evol Microbiol;67(8):2941-2946, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Four novel Lipomyces strains were isolated from soil collected in the Okinawa and Yamanashi prefectures, Japan. Based on their morphological and biochemical characteristics, along with sequence typing using the D1/D2 domain of the LSU rRNA, internal transcribed spacer (ITS) region including 5.8S rRNA, and translation elongation factor 1 alpha gene (EF-1α), the four strains were shown to represent two novel species of the genus Lipomyces, described as Lipomyces okinawensis sp. nov. (type strain No.3-a(35)T=NBRC 110620T=CBS 14747T) and Lipomyces yamanashiensis f.a., sp. nov. (type strain No.313T=NBRC 110621T=CBS 14748T).
[Mh] Termos MeSH primário: Lipomyces/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: DNA Fúngico/genética
DNA Espaçador Ribossômico/genética
Genes Fúngicos
Japão
Lipomyces/genética
Lipomyces/isolamento & purificação
Técnicas de Tipagem Micológica
Fator 1 de Elongação de Peptídeos/genética
RNA Ribossômico/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal Spacer); 0 (Peptide Elongation Factor 1); 0 (RNA, Ribosomal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002050


  9 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28801462
[Au] Autor:Jank T; Belyi Y; Wirth C; Rospert S; Hu Z; Dengjel J; Tzivelekidis T; Andersen GR; Hunte C; Schlosser A; Aktories K
[Ad] Endereço:From the Institute for Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany, thomas.jank@pharmakol.uni-freiburg.de.
[Ti] Título:Protein glutaminylation is a yeast-specific posttranslational modification of elongation factor 1A.
[So] Source:J Biol Chem;292(39):16014-16023, 2017 Sep 29.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ribosomal translation factors are fundamental for protein synthesis and highly conserved in all kingdoms of life. The essential eukaryotic elongation factor 1A (eEF1A) delivers aminoacyl tRNAs to the A-site of the translating 80S ribosome. Several studies have revealed that eEF1A is posttranslationally modified. Using MS analysis, site-directed mutagenesis, and X-ray structural data analysis of eEF1A, we identified a posttranslational modification in which the α amino group of mono-l-glutamine is covalently linked to the side chain of glutamate 45 in eEF1A. The MS analysis suggested that all eEF1A molecules are modified by this glutaminylation and that this posttranslational modification occurs at all stages of yeast growth. The mutational studies revealed that this glutaminylation is not essential for the normal functions of eEF1A in However, eEF1A glutaminylation slightly reduced growth under antibiotic-induced translational stress conditions. Moreover, we identified the same posttranslational modification in eEF1A from but not in various other eukaryotic organisms tested despite strict conservation of the Glu residue among these organisms. We therefore conclude that eEF1A glutaminylation is a yeast-specific posttranslational modification that appears to influence protein translation.
[Mh] Termos MeSH primário: Glutamina/metabolismo
Modelos Moleculares
Fator 1 de Elongação de Peptídeos/metabolismo
Processamento de Proteína Pós-Traducional
Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Substituição de Aminoácidos
Aminoacilação/efeitos dos fármacos
Anti-Infecciosos/farmacologia
Sequência Conservada
Cristalografia por Raios X
Bases de Dados de Proteínas
Regulação Fúngica da Expressão Gênica/efeitos dos fármacos
Ácido Glutâmico/metabolismo
Sequências Hélice-Alça-Hélice
Mutagênese Sítio-Dirigida
Mutação
Fator 1 de Elongação de Peptídeos/química
Fator 1 de Elongação de Peptídeos/genética
Processamento de Proteína Pós-Traducional/efeitos dos fármacos
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/metabolismo
Saccharomyces cerevisiae/efeitos dos fármacos
Saccharomyces cerevisiae/crescimento & desenvolvimento
Proteínas de Saccharomyces cerevisiae/química
Proteínas de Saccharomyces cerevisiae/genética
Alinhamento de Sequência
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Peptide Elongation Factor 1); 0 (Recombinant Fusion Proteins); 0 (Saccharomyces cerevisiae Proteins); 0 (TEF2 protein, S cerevisiae); 0RH81L854J (Glutamine); 3KX376GY7L (Glutamic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170813
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.801035


  10 / 1933 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28721840
[Au] Autor:Naumov GI; Naumova ES; Lee CF
[Ad] Endereço:1​State Institute for Genetics and Selection of Industrial Microorganisms, Moscow 117545, Russia.
[Ti] Título:Ogataea haglerorum sp. nov., a novel member of the species complex, Ogataea (Hansenula) polymorpha.
[So] Source:Int J Syst Evol Microbiol;67(7):2465-2469, 2017 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Three strains representing a novel species of the Ogataea clade were isolated by W. T. Starmer and H. J. Phaff from rotting tissue of Opuntia phaeacantha in Arizona, USA. Analyses of the sequences of the D1/D2 LSU rRNA gene, ITS1-5.8S-ITS2, and translation elongation factor-1α (EF-1 α) showed that this novel species belongs to the Ogataea polymorpha complex formed by Ogataea angusta, Ogataea parapolymorpha and Ogataea polymorpha. The novel species differs from these species by 4-5 nucleotide substitutions in the D1/D2 domain, by 28-29 nucleotide substitutions in the EF-α gene and by 18-24 nucleotide substitutions and 2-5 indels in the ITS-5.8S region. The name Ogataea haglerorum sp. nov. is proposed for this novel species. The type strain is VKPM Y-2583T (=CBS 14645T=UCDFST 17-101T). The Mycobank number is MB 819772.
[Mh] Termos MeSH primário: Opuntia/microbiologia
Filogenia
Saccharomycetales/classificação
[Mh] Termos MeSH secundário: Arizona
DNA Fúngico/genética
DNA Espaçador Ribossômico/genética
Genes de RNAr
Técnicas de Tipagem Micológica
Fator 1 de Elongação de Peptídeos/genética
Saccharomycetales/genética
Saccharomycetales/isolamento & purificação
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal Spacer); 0 (Peptide Elongation Factor 1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002012



página 1 de 194 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde