Base de dados : MEDLINE
Pesquisa : D08.811.277.087 [Categoria DeCS]
Referências encontradas : 8488 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 849 ir para página                         

  1 / 8488 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29233653
[Au] Autor:Ding S; Dai RY; Wang WK; Cao Q; Lan LF; Zhou XL; Yang YS
[Ad] Endereço:Key Laboratory of New Drug Research and Development of Liaoning Province, College of Pharmacy of Liaoning University, Shenyang 10036, Liaoning Province, China; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
[Ti] Título:Design, synthesis and structure-activity relationship evaluation of novel LpxC inhibitors as Gram-negative antibacterial agents.
[So] Source:Bioorg Med Chem Lett;28(2):94-102, 2018 01 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:LpxC inhibitors are new-type antibacterial agents developed in the last twenty years, mainly against Gram-negative bacteria infections. To develop novel LpxC inhibitors with good antibacterial activities and biological metabolism, we summarized the basic skeleton of reported LpxC inhibitors, designed and synthesized several series of compounds and tested their antibacterial activities against Escherichial coli and Pseudomonas aeruginosa in vitro. Structure-activity relationships have been discussed in this article. The metabolism stability of YDL-2, YDL-5, YDL-8, YDL-14, YDL-20-YDL-23 have been evaluated in liver microsomes, which indicated that the 2-amino isopropyl group may be a preferred structure than the 2-hydroxy ethyl group in the design of LpxC inhibitors.
[Mh] Termos MeSH primário: Amidoidrolases/antagonistas & inibidores
Antibacterianos/farmacologia
Desenho de Drogas
Inibidores Enzimáticos/farmacologia
Escherichia coli/efeitos dos fármacos
Pseudomonas aeruginosa/efeitos dos fármacos
[Mh] Termos MeSH secundário: Amidoidrolases/metabolismo
Antibacterianos/síntese química
Antibacterianos/química
Relação Dose-Resposta a Droga
Inibidores Enzimáticos/síntese química
Inibidores Enzimáticos/química
Testes de Sensibilidade Microbiana
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Enzyme Inhibitors); EC 3.5.- (Amidohydrolases); EC 3.5.1.- (LpxC deacetylase, Pseudomonas); EC 3.5.1.- (UDP-3-O-acyl-N-acetylglucosamine deacetylase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE


  2 / 8488 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28453015
[Au] Autor:Cerqueira NMFSA; Moorthy H; Fernandes PA; Ramos MJ
[Ad] Endereço:UCIBO-REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, 4169-007 Porto, Portugal. nscerque@fc.up.pt.
[Ti] Título:The mechanism of the Ser-(cis)Ser-Lys catalytic triad of peptide amidases.
[So] Source:Phys Chem Chem Phys;19(19):12343-12354, 2017 May 21.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this paper, we report a theoretical investigation of the catalytic mechanism of peptide amidases that involve a Ser-(cis)Ser-Lys catalytic triad. Previous suggestions propose that these enzymes should follow a distinct catalytic mechanism from the one that is present in the classic Ser-His-Asp catalytic triad. The theoretical and computational results obtained in this work indicate the opposite idea, showing that both mechanisms are very similar and only few differences are observed between both reactions. The results reveal that the different alignment of the Ser-(cis)Ser-Lys catalytic triad in relation to the classical Ser-His-Asp triad may provide a better stabilisation of the reaction intermediates, and therefore make these enzymes catalytically more efficient. The catalytic mechanism has been determined at the M06-2X/6-311++G**//B3LYP/6-31G* level of theory and requires five sequential steps instead of the two that are generally proposed: (i) nucleophilic attack of serine on the carbonyl group of the substrate, forming the first tetrahedral intermediate, (ii) formation of an acyl-enzyme complex, (ii) release of an ammonia product, (iv) nucleophilic attack of a water molecule forming the second tetrahedral intermediate, and (iv) the release of the product of the reaction, the carboxylic acid. The computational results suggest that the rate-limiting step is the first one that requires an activation free energy of 15.93 kcal mol . This result agrees very well with the available experimental data that predict a reaction rate of 2200 s , which corresponds to a free energy barrier of 14 kcal mol .
[Mh] Termos MeSH primário: Amidoidrolases/química
Amidoidrolases/metabolismo
Modelos Químicos
Peptídeos/química
[Mh] Termos MeSH secundário: Catálise
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptides); 059QF0KO0R (Water); EC 3.5.- (Amidohydrolases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp00277g


  3 / 8488 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28470883
[Au] Autor:Schardon CL; Tuley A; Er JAV; Swartzel JC; Fast W
[Ad] Endereço:Biochemistry Graduate Program, The University of Texas, Austin, TX, 78712, USA.
[Ti] Título:Selective Covalent Protein Modification by 4-Halopyridines through Catalysis.
[So] Source:Chembiochem;18(15):1551-1556, 2017 08 04.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We have investigated 4-halopyridines as selective, tunable, and switchable covalent protein modifiers for use in the development of chemical probes. Nonenzymatic reactivity of 4-chloropyridine with amino acids and thiols was ranked with respect to common covalent protein-modifying reagents and found to have reactivity similar to that of acrylamide, but could be switched to a reactivity similar to that of iodoacetamide upon stabilization of the positively charged pyridinium. Diverse, fragment-sized 4-halopyridines inactivated human dimethylarginine dimethylaminohydrolase-1 (DDAH1) through covalent modification of the active site cysteine, acting as quiescent affinity labels that required off-pathway catalysis through stabilization of the protonated pyridinium by a neighboring aspartate residue. A series of 2-fluoromethyl-substituted 4-chloropyridines demonstrated that the pK and k /K values could be predictably varied over several orders of magnitude. Covalent labeling of proteins in an Escherichia coli lysate was shown to require folded proteins, indicating that alternative proteins can be targeted, and modification is likely to be catalysisdependent. 4-Halopyridines, and quiescent affinity labels in general, represent an attractive strategy to develop reagents with switchable electrophilicity as selective covalent protein modifiers.
[Mh] Termos MeSH primário: Amidoidrolases/química
Piridinas/química
[Mh] Termos MeSH secundário: Acrilamida/química
Marcadores de Afinidade/química
Cisteína/química
Escherichia coli/metabolismo
Glutationa/química
Seres Humanos
Iodoacetamida/química
Fenóis/química
Proteoma/química
Proteoma/metabolismo
Compostos de Piridínio/química
Compostos de Sulfidrila/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Affinity Labels); 0 (Phenols); 0 (Proteome); 0 (Pyridines); 0 (Pyridinium Compounds); 0 (Sulfhydryl Compounds); 20R035KLCI (Acrylamide); 7K011JR4T0 (thiophenol); EC 3.5.- (Amidohydrolases); EC 3.5.3.18 (dimethylargininase); GAN16C9B8O (Glutathione); K848JZ4886 (Cysteine); ZRH8M27S79 (Iodoacetamide)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201700104


  4 / 8488 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28448444
[Au] Autor:Mariani F; Roncucci L
[Ad] Endereço:Department of Diagnostic and Clinical Medicine, and Public Health, University of Modena and Reggio Emilia, Via Del Pozzo 71, I-41125 Modena, Italy. francesco.mariani@unimore.it.
[Ti] Título:Role of the Vanins-Myeloperoxidase Axis in Colorectal Carcinogenesis.
[So] Source:Int J Mol Sci;18(5), 2017 Apr 27.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The presence of chronic inflammation in the colonic mucosa leads to an increased risk of cancer. Among proteins involved in the regulation of mucosal inflammation and that may contribute both to structural damage of the intestinal mucosa and to intestinal carcinogenesis, there are myeloperoxidase (MPO) and vanins. The infiltration of colonic mucosa by neutrophils may promote carcinogenesis through MPO, a key enzyme contained in the lysosomes of neutrophils that regulates local inflammation and the generation of reactive oxygen species (ROS) and mutagenic species. The human vanin gene family consists of three genes: , and . All vanin molecules are pantetheinases, that hydrolyze pantetheine into pantothenic acid (vitamin B5), and cysteamine, a sulfhydryl compound. Vanin-1 loss confers an increased resistance to stress and acute intestinal inflammation, while vanin-2 regulates adhesion and transmigration of activated neutrophils. The metabolic product of these enzymes has a prominent role in the inflammation processes by affecting glutathione levels, inducing ulcers through a reduction in mucosal blood flow and oxygenation, decreasing local defense mechanisms, and in carcinogenesis by damaging DNA and regulating pathways involved in cell apoptosis, metabolism and growth, as Nrf2 and HIF-1α.
[Mh] Termos MeSH primário: Amidoidrolases/metabolismo
Neoplasias Colorretais/patologia
Peroxidase/metabolismo
[Mh] Termos MeSH secundário: Amidoidrolases/antagonistas & inibidores
Amidoidrolases/genética
Carcinogênese
Neoplasias Colorretais/tratamento farmacológico
Neoplasias Colorretais/metabolismo
Cisteamina/metabolismo
Inibidores Enzimáticos/uso terapêutico
Seres Humanos
Inflamação
Peroxidase/antagonistas & inibidores
Peroxidase/genética
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Reactive Oxygen Species); 5UX2SD1KE2 (Cysteamine); EC 1.11.1.7 (Peroxidase); EC 3.5.- (Amidohydrolases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE


  5 / 8488 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28455333
[Au] Autor:Kusada H; Tamaki H; Kamagata Y; Hanada S; Kimura N
[Ad] Endereço:Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
[Ti] Título:A Novel Quorum-Quenching -Acylhomoserine Lactone Acylase from Acidovorax sp. Strain MR-S7 Mediates Antibiotic Resistance.
[So] Source:Appl Environ Microbiol;83(13), 2017 Jul 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:-Acylhomoserine lactone acylase (AHL acylase) is a well-known enzyme responsible for disrupting cell-cell communication (quorum sensing) in bacteria. Here, we isolated and characterized a novel and unique AHL acylase (designated MacQ) from a multidrug-resistant bacterium, sp. strain MR-S7. The purified MacQ protein heterologously expressed in degraded a wide variety of AHLs, ranging from C to C side chains with or without 3-oxo substitutions. We also observed that AHL-mediated virulence factor production in a plant pathogen, , was dramatically attenuated by coculture with MacQ-overexpressing , whereas with an empty vector was unable to quench the pathogenicity, which strongly indicates that MacQ can act as a quorum-quenching enzyme and interfere with the quorum-sensing system in the pathogen. In addition, this enzyme was found to be capable of degrading a wide spectrum of ß-lactams (penicillin G, ampicillin, amoxicillin, carbenicillin, cephalexin, and cefadroxil) by deacylation, clearly indicating that MacQ is a bifunctional enzyme that confers both quorum quenching and antibiotic resistance on strain MR-S7. MacQ has relatively low amino acid sequence identity to any of the known acylases (<39%) and has among the broadest substrate range. Our findings provide the possibility that AHL acylase genes can be an alternative source of antibiotic resistance genes posing a threat to human health if they migrate and transfer to pathogenic bacteria. -Acylhomoserine lactones (AHLs) are well-known signal molecules for bacterial cell-cell communication (quorum sensing), and AHL acylase, which is able to degrade AHLs, has been recognized as a major target for quorum-sensing interference (quorum quenching) in pathogens. In this work, we succeeded in isolating a novel AHL acylase (MacQ) from a multidrug-resistant bacterium and demonstrated that the MacQ enzyme could confer multidrug resistance as well as quorum quenching on the host organism. Indeed, the purified MacQ protein was found to be bifunctional and capable of degrading not only various AHL derivatives but also multiple ß-lactam antibiotics by deacylation activities. Although quorum quenching and antibiotic resistance have been recognized to be distinct biological functions, our findings clearly link the two functions by discovering the novel bifunctional enzyme and further providing the possibility that a hitherto-overlooked antibiotic resistance mechanism mediated by the quorum-quenching enzyme may exist in natural environments and perhaps in clinical settings.
[Mh] Termos MeSH primário: Amidoidrolases/metabolismo
Comamonadaceae/enzimologia
Farmacorresistência Bacteriana
[Mh] Termos MeSH secundário: Acil-Butirolactonas/metabolismo
Amidoidrolases/genética
Antibacterianos/metabolismo
Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Comamonadaceae/efeitos dos fármacos
Comamonadaceae/genética
Comamonadaceae/fisiologia
Escherichia coli/genética
Escherichia coli/metabolismo
Regulação Bacteriana da Expressão Gênica
Percepção de Quorum
beta-Lactamas/metabolismo
beta-Lactamas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acyl-Butyrolactones); 0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (beta-Lactams); EC 3.5.- (Amidohydrolases); EC 3.5.1.4 (amidase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  6 / 8488 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27771531
[Au] Autor:Sartim AG; Moreira FA; Joca SRL
[Ad] Endereço:Department of Physical and Chemical, School of Pharmaceutical Science of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
[Ti] Título:Involvement of CB and TRPV1 receptors located in the ventral medial prefrontal cortex in the modulation of stress coping behavior.
[So] Source:Neuroscience;340:126-134, 2017 Jan 06.
[Is] ISSN:1873-7544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cannabinoid type-1 (CB ) and transient receptor potential vanilloid type-1 (TRPV1) receptors may have opposite roles in modulating neural activity and, consequently, in regulating the stress response. These receptors are widely expressed in several brain structures, including the ventral medial prefrontal cortex (vmPFC). The functional consequences of the interaction between CB and TRPV1, however, have scarcely been explored. Therefore, we investigated if CB and TRPV1 receptors located in the vmPFC would be involved in the behavioral changes induced by the stress of the forced swim test (FST). Rats with cannulae implanted into the vmPFC were given the dual blocker of TRPV1 receptors and fatty acid amide hydrolase (FAAH), Arachidonyl serotonin (AA-5HT, 0.125/0.25/0.5nmol), TRPV1 antagonist, SB366791 (0.5/1/10nmol), FAAH inhibitor, URB597 (0.001/0.01/0.1/1nmol), or vehicle and were submitted to the FST, or to the open-field test. Another group received intra-vmPFC injection of SB366791 or vehicle, followed by a second injection of URB597 or vehicle, and was submitted to the FST. Lastly, a group received intra-vmPFC injection of a CB antagonist, in sub-effective dose or vehicle, followed by AA-5HT, SB366791 or vehicle. The results showed that AA-5HT, SB366791 and URB597 significantly reduced the immobility time without changing the locomotor activity. Furthermore, the co-administration of URB597 and SB366791 in sub-effective doses induced an antidepressant-like effect in the FST. Additionally, the antidepressant-like effect of AA-5HT was prevented by the CB antagonist. Together, these results suggest that both, CB and TRPV1 receptors located in the vmPFC are involved in the behavioral responses to stress, although in opposite ways.
[Mh] Termos MeSH primário: Adaptação Psicológica/fisiologia
Córtex Pré-Frontal/metabolismo
Receptor CB1 de Canabinoide/metabolismo
Estresse Psicológico/metabolismo
Canais de Cátion TRPV/metabolismo
[Mh] Termos MeSH secundário: Adaptação Psicológica/efeitos dos fármacos
Amidoidrolases/antagonistas & inibidores
Amidoidrolases/metabolismo
Anilidas/farmacologia
Animais
Antidepressivos/farmacologia
Ácidos Araquidônicos/farmacologia
Benzamidas/farmacologia
Carbamatos/farmacologia
Cinamatos/farmacologia
Masculino
Atividade Motora/efeitos dos fármacos
Atividade Motora/fisiologia
Neurotransmissores/farmacologia
Córtex Pré-Frontal/efeitos dos fármacos
Ratos Wistar
Serotonina/análogos & derivados
Serotonina/farmacologia
Canais de Cátion TRPV/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anilides); 0 (Antidepressive Agents); 0 (Arachidonic Acids); 0 (Benzamides); 0 (Carbamates); 0 (Cinnamates); 0 (Cnr1 protein, rat); 0 (N-(3-methoxyphenyl)-4-chlorocinnamanilide); 0 (Neurotransmitter Agents); 0 (Receptor, Cannabinoid, CB1); 0 (TRPV Cation Channels); 0 (Trpv1 protein, rat); 0 (arachidonyl serotonin); 0 (cyclohexyl carbamic acid 3'-carbamoylbiphenyl-3-yl ester); 333DO1RDJY (Serotonin); EC 3.5.- (Amidohydrolases); EC 3.5.1.- (fatty-acid amide hydrolase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171224
[Lr] Data última revisão:
171224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  7 / 8488 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28743542
[Au] Autor:Maia J; Almada M; Silva A; Correia-da-Silva G; Teixeira N; Sá SI; Fonseca BM
[Ad] Endereço:UCIBIO, REQUIMTE, Departamento de Ciências Biológicas, Laboratório de Bioquímica, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.
[Ti] Título:The endocannabinoid system expression in the female reproductive tract is modulated by estrogen.
[So] Source:J Steroid Biochem Mol Biol;174:40-47, 2017 11.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The endocannabinoid system (ECS) is involved in several physiological events that resulted in a growing interest in its modulation. Moreover, the uterine levels of anandamide (AEA), the major endocannabinoid, must be tightly regulated to create proper embryo implantation conditions. However, there are no evidences about the regulation of AEA in uterus by estrogen. Thus, the aim of this study is to elucidate whether estradiol benzoate (EB) and tamoxifen (TAM) administration to ovariectomized (OVX) rats can induce changes in the expression of cannabinoid receptors and AEA-metabolic enzymes in uterus by evaluating gene transcription and protein levels by qPCR, Western blot and immunohistochemistry. Moreover, the plasmatic and uterine levels of AEA and of prostaglandin E (PGE ) and prostaglandin F α (PGF ), the major cyclooxygenase-2 (COX-2) products, were determined by UPLC-MS/MS. The immunohistochemistry showed that cannabinoid receptors, as well as AEA-metabolic enzymes are mainly located in the epithelial cells of both lumen and glands and, to a lesser extent, in the muscle cells. Moreover, EB administration to OVX rats significantly increased CB1, CB2, NAPE-PLD, FAAH and COX-2 expression and transcription. These effects were absent in TAM and TAM+EB treatments showing that this response is estrogen receptor dependent. Additionally, although uterine levels of AEA remained unchanged in EB or TAM treated animals, they showed a rise with EB treatment in plasma. The latter also produced a decrease in uterine PGE levels. In summary, these data collectively indicate that the expression of ECS components, as well as, the AEA and PGE levels in rat uterus is modulated by EB. Thus, estradiol may have a direct regulatory role in the modulation of ECS in female reproductive tissues.
[Mh] Termos MeSH primário: Estradiol/análogos & derivados
Estrogênios/farmacologia
Tamoxifeno/farmacologia
Útero/efeitos dos fármacos
[Mh] Termos MeSH secundário: Amidoidrolases/genética
Amidoidrolases/metabolismo
Animais
Ácidos Araquidônicos/sangue
Ácidos Araquidônicos/metabolismo
Ciclo-Oxigenase 2/genética
Ciclo-Oxigenase 2/metabolismo
Dinoprosta/sangue
Dinoprostona/sangue
Dinoprostona/metabolismo
Endocanabinoides/sangue
Endocanabinoides/metabolismo
Estradiol/farmacologia
Feminino
Tamanho do Órgão/efeitos dos fármacos
Ovariectomia
Fosfolipase D/genética
Fosfolipase D/metabolismo
Alcamidas Poli-Insaturadas/sangue
Alcamidas Poli-Insaturadas/metabolismo
Ratos Wistar
Receptor CB1 de Canabinoide/genética
Receptor CB1 de Canabinoide/metabolismo
Receptor CB2 de Canabinoide/genética
Receptor CB2 de Canabinoide/metabolismo
Útero/metabolismo
Útero/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arachidonic Acids); 0 (Endocannabinoids); 0 (Estrogens); 0 (Polyunsaturated Alkamides); 0 (Receptor, Cannabinoid, CB1); 0 (Receptor, Cannabinoid, CB2); 094ZI81Y45 (Tamoxifen); 1S4CJB5ZGN (estradiol 3-benzoate); 4TI98Z838E (Estradiol); B7IN85G1HY (Dinoprost); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.14.99.1 (Ptgs2 protein, rat); EC 3.1.4.4 (NAPE-PLD protein, rat); EC 3.1.4.4 (Phospholipase D); EC 3.5.- (Amidohydrolases); EC 3.5.1.- (fatty-acid amide hydrolase); K7Q1JQR04M (Dinoprostone); UR5G69TJKH (anandamide)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171208
[Lr] Data última revisão:
171208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


  8 / 8488 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28470834
[Au] Autor:Du S; Lutkenhaus J
[Ad] Endereço:Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, 66160, USA.
[Ti] Título:The N-succinyl-l,l-diaminopimelic acid desuccinylase DapE acts through ZapB to promote septum formation in Escherichia coli.
[So] Source:Mol Microbiol;105(2):326-345, 2017 Jul.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spatial regulation of cell division in Escherichia coli occurs at the stage of Z ring formation. It consists of negative (the Min and NO systems) and positive (Ter signal mediated by MatP/ZapA/ZapB) regulators. Here, we find that N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) facilitates functional Z ring formation by strengthening the Ter signal via ZapB. DapE depends on ZapB to localize to the Z ring and its overproduction suppresses the division defect caused by loss of both the Min and NO systems. DapE shows a strong interaction with ZapB and requires the presence of ZapB to exert its function in division. Consistent with the idea that DapE strengthens the Ter signal, overproduction of DapE supports cell division with reduced FtsZ levels and provides some resistance to the FtsZ inhibitors MinCD and SulA, while deletion of dapE, like deletion of zapB, exacerbates the phenotypes of cells impaired in Z ring formation such as ftsZ84 or a min mutant. Taken together, our results report DapE as a new component of the divisome that promotes the integrity of the Z ring by acting through ZapB and raises the possibility of the existence of additional divisome proteins that also function in other cellular processes.
[Mh] Termos MeSH primário: Amidoidrolases/genética
Amidoidrolases/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Proteínas de Transporte/metabolismo
Proteínas de Ciclo Celular/metabolismo
Divisão Celular
Proteínas do Citoesqueleto/metabolismo
Ácido Diaminopimélico
Escherichia coli/genética
Proteínas de Escherichia coli/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Carrier Proteins); 0 (Cell Cycle Proteins); 0 (Cytoskeletal Proteins); 0 (Escherichia coli Proteins); 583-93-7 (Diaminopimelic Acid); EC 3.5.- (Amidohydrolases); EC 3.5.1.18 (succinyldiaminopimelate desuccinylase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.13703


  9 / 8488 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29088294
[Au] Autor:Ngabonziza JCS; Diallo AB; Tagliani E; Diarra B; Kadanga AE; Togo ACG; Thiam A; de Rijk WB; Alagna R; Houeto S; Ba F; Dagnra AY; Ivan E; Affolabi D; Schwoebel V; Trebucq A; de Jong BC; Rigouts L; Daneau G; "Union short MDR-TB regimen study group"
[Ad] Endereço:National Reference Laboratory Division, Biomedical Services Department, Rwanda Biomedical Centre, Kigali, Rwanda.
[Ti] Título:Half of rifampicin-resistant Mycobacterium tuberculosis complex isolated from tuberculosis patients in Sub-Saharan Africa have concomitant resistance to pyrazinamide.
[So] Source:PLoS One;12(10):e0187211, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Besides inclusion in 1st line regimens against tuberculosis (TB), pyrazinamide (PZA) is used in 2nd line anti-TB regimens, including in the short regimen for multidrug-resistant TB (MDR-TB) patients. Guidelines and expert opinions are contradictory about inclusion of PZA in case of resistance. Moreover, drug susceptibility testing (DST) for PZA is not often applied in routine testing, and the prevalence of resistance is unknown in several regions, including in most African countries. METHODS: Six hundred and twenty-three culture isolates from rifampicin-resistant (RR) patients were collected in twelve Sub-Saharan African countries. Among those isolates, 71% were from patients included in the study on the Union short-course regimen for MDR-TB in Benin, Burkina Faso, Burundi, Cameroon, Central Africa Republic, the Democratic Republic of the Congo, Ivory Coast, Niger, and Rwanda PZA resistance, and the rest (29%) were consecutive isolates systematically stored from 2014-2015 in Mali, Rwanda, Senegal, and Togo. Besides national guidelines, the isolates were tested for PZA resistance through pncA gene sequencing. RESULTS: Over half of these RR-TB isolates (54%) showed a mutation in the pncA gene, with a significant heterogeneity between countries. Isolates with fluoroquinolone resistance (but not with injectable resistance or XDR) were more likely to have concurrent PZA resistance. The pattern of mutations in the pncA gene was quite diverse, although some isolates with an identical pattern of mutations in pncA and other drug-related genes were isolated from the same reference center, suggesting possible transmission of these strains. CONCLUSION: Similar to findings in other regions, more than half of the patients having RR-TB in West and Central Africa present concomitant resistance to PZA. Further investigations are needed to understand the relation between resistance to PZA and resistance to fluoroquinolones, and whether continued use of PZA in the face of PZA resistance provides clinical benefit to the patients.
[Mh] Termos MeSH primário: Antituberculosos/uso terapêutico
Mycobacterium tuberculosis/efeitos dos fármacos
Pirazinamida/uso terapêutico
Rifampina/uso terapêutico
Tuberculose Pulmonar/tratamento farmacológico
[Mh] Termos MeSH secundário: Adolescente
Adulto
África ao Sul do Saara/epidemiologia
Idoso
Idoso de 80 Anos ou mais
Amidoidrolases/genética
Criança
Farmacorresistência Bacteriana Múltipla/genética
Feminino
Seres Humanos
Masculino
Meia-Idade
Mycobacterium tuberculosis/genética
Tuberculose Pulmonar/microbiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antitubercular Agents); 2KNI5N06TI (Pyrazinamide); EC 3.5.- (Amidohydrolases); EC 3.5.- (PncA protein, Mycobacterium tuberculosis); VJT6J7R4TR (Rifampin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171101
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187211


  10 / 8488 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28910408
[Au] Autor:Karlsson J; Gouveia-Figueira S; Alhouayek M; Fowler CJ
[Ad] Endereço:Department of Pharmacology and Clinical Neuroscience, Pharmacology Unit, Umeå University, Umeå, Sweden.
[Ti] Título:Effects of tumour necrosis factor α upon the metabolism of the endocannabinoid anandamide in prostate cancer cells.
[So] Source:PLoS One;12(9):e0185011, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tumour necrosis factor α (TNFα) is involved in the pathogenesis of prostate cancer, a disease where disturbances in the endocannabinoid system are seen. In the present study we have investigated whether treatment of DU145 human prostate cancer cells affects anandamide (AEA) catabolic pathways. Additionally, we have investigated whether cyclooxygenase-2 (COX-2) can regulate the uptake of AEA into cells. Levels of AEA synthetic and catabolic enzymes were determined by qPCR. AEA uptake and hydrolysis in DU145 and RAW264.7 macrophage cells were assayed using AEA labeled in the arachidonic and ethanolamine portions of the molecule, respectively. Levels of AEA, related N-acylethanolamines (NAEs), prostaglandins (PG) and PG-ethanolamines (PG-EA) in DU145 cells and medium were quantitated by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. TNFα treatment of DU145 cells increased mRNA levels of PTSG2 (gene of COX-2) and decreased the mRNA of the AEA synthetic enzyme N-acyl-phosphatidylethanolamine selective phospholipase D. mRNA levels of the AEA hydrolytic enzymes fatty acid amide hydrolase (FAAH) and N-acylethanolamine-hydrolyzing acid amidase were not changed. AEA uptake in both DU145 and RAW264.7 cells was inhibited by FAAH inhibition, but not by COX-2 inhibition, even in RAW264.7 cells where the expression of this enzyme had greatly been induced by lipopolysaccharide + interferon γ treatment. AEA and related NAEs were detected in DU145 cells, but PGs and PGE2-EA were only detected when the cells had been preincubated with 100 nM AEA. The data demonstrate that in DU145 cells, TNFα treatment changes the relative expression of the enzymes involved in the hydrolytic and oxygenation catabolic pathways for AEA. In RAW264.7 cells, COX-2, in contrast to FAAH, does not regulate the cellular accumulation of AEA. Further studies are necessary to determine the extent to which inflammatory mediators are involved in the abnormal endocannabinoid signalling system in prostate cancer.
[Mh] Termos MeSH primário: Amidoidrolases/genética
Ácidos Araquidônicos/análise
Ciclo-Oxigenase 2/genética
Endocanabinoides/análise
Alcamidas Poli-Insaturadas/análise
Neoplasias da Próstata/metabolismo
Fator de Necrose Tumoral alfa/farmacologia
[Mh] Termos MeSH secundário: Amidoidrolases/metabolismo
Animais
Linhagem Celular Tumoral
Cromatografia Líquida de Alta Pressão
Ciclo-Oxigenase 2/metabolismo
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Lipopolissacarídeos/efeitos adversos
Masculino
Camundongos
Prostaglandinas/análise
Neoplasias da Próstata/genética
Células RAW 264.7
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arachidonic Acids); 0 (Endocannabinoids); 0 (Lipopolysaccharides); 0 (Polyunsaturated Alkamides); 0 (Prostaglandins); 0 (Tumor Necrosis Factor-alpha); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.14.99.1 (PTGS2 protein, human); EC 3.5.- (Amidohydrolases); EC 3.5.1.- (fatty-acid amide hydrolase); UR5G69TJKH (anandamide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185011



página 1 de 849 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde