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[PMID]:28320396
[Au] Autor:Ingti B; Paul D; Maurya AP; Bora D; Chanda DD; Chakravarty A; Bhattacharjee A
[Ad] Endereço:Department of Microbiology, Assam University, Silchar, 788011, India.
[Ti] Título:Occurrence of bla mediated cephalosporin resistance in Escherichia coli and their transcriptional response against cephalosporin stress: a report from India.
[So] Source:Ann Clin Microbiol Antimicrob;16(1):13, 2017 Mar 21.
[Is] ISSN:1476-0711
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Treatment alternatives for DHA-1 harboring strains are challenging as it confers resistance to broad spectrum cephalosporins and may further limit treatment option when expressed at higher levels. Therefore, this study was designed to know the prevalence of DHA genes and analyse the transcription level of DHA-1 against different ß-lactam stress. METHODS: Screening of AmpC ß-lactamase phenotypically by modified three dimensional extract method followed by Antimicrobial Susceptibility and MIC determination. Genotyping screening of ß-lactamase genes was performed by PCR assay followed by their sequencing. The bla transcriptional response was evaluated under different cephalosporin stress by RT PCR. Transferability of bla gene was performed by transformation and conjugation and plasmid incompatibility typing, DNA fingerprinting by enterobacterial repetitive intergenic consensus sequences PCR. RESULTS: 16 DHA-1 genes were screened positive from 176 Escherichia coli isolates and primer extension analysis showed a significant increase in DHA-1 mRNA transcription in response to cefotaxime at 8 µg/ml (6.99 × 10 fold), ceftriaxone at 2 µg/ml (2.63 × 10 fold), ceftazidime at 8 µg/ml (7.06 × 10 fold) and cefoxitin at 4 µg/ml (3.60 × 10 fold) when compared with untreated strain. These transcription data were found significant when analyzed statistically using one way ANOVA. Four different ESBL genes were detected in 10 isolates which include CTX-M (n = 6), SHV (n = 4), TEM (n = 3) and OXA-10 (n = 1), whereas, carbapenemase gene (NDM) was detected only in one isolate. Other plasmid mediated AmpC ß-lactamases CIT (n = 9), EBC (n = 2) were detected in nine isolates. All DHA-1 genes detected were encoded in plasmid and incompatibility typing from the transformants indicated that the plasmid encoding bla was carried mostly by the FIA and L/M Inc group. CONCLUSION: This study demonstrates the prevalence of DHA-1 gene in this region and highlights high transcription of DHA-1 when induced with different ß-lactam antibiotics. Therefore, cephalosporin treatment must be restricted for the patients infected with pathogen expressing this resistance determinant.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Resistência às Cefalosporinas
Cefalosporinase/biossíntese
Cefalosporinas/farmacologia
Infecções por Escherichia coli/microbiologia
Proteínas de Escherichia coli/biossíntese
Escherichia coli/enzimologia
Transcrição Genética/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adulto
Idoso de 80 Anos ou mais
Cefalosporinase/genética
Cefalosporinase/secreção
Conjugação Genética
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Infecções por Escherichia coli/epidemiologia
Proteínas de Escherichia coli/genética
Proteínas de Escherichia coli/secreção
Feminino
Perfilação da Expressão Gênica
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Transferência Genética Horizontal
Técnicas de Genotipagem
Seres Humanos
Índia/epidemiologia
Masculino
Testes de Sensibilidade Microbiana
Prevalência
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephalosporins); 0 (Escherichia coli Proteins); EC 3.5.2.- (Cephalosporinase); EC 3.5.2.- (DHA-1 cephalosporinase, E coli)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1186/s12941-017-0189-x


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[PMID]:28219797
[Au] Autor:Shi P; Qiao P; Zhang Y; Li S; Feng X; Bian L
[Ad] Endereço:College of Life Science, Northwest University, Xi'an 710069, China.
[Ti] Título:Spectroscopy analysis and molecular dynamics studies on the binding of penicillin V and sulbactam to beta-lactamase II from Bacillus cereus.
[So] Source:J Pharm Biomed Anal;138:206-214, 2017 May 10.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The molecular recognition and interaction of beta-lactamase II from Bacillus cereus (Bc II) with penicillin V (PV) and sulbactam (Sul) especially conformational changes of Bc II in the binding process were studied through spectroscopy analysis in combination with molecular dynamics (MD) simulation. The results show that in the binding process, a new coordination bond is observed between the Zn of Bc II and the carboxyl-O of PV or Sul by replacing His204. Electrostatic interaction between Zn and the ligand provide main driving force for the binding affinity. Compared with apo Bc II, there are mainly four loops showing significant conformational changes in ligand-bound Bc II. A weak conformational transformation from ß-sheets to random coils is observed in the loop2 of ligand-bound Bc II. The conformational transformation may depend on the functional group and binding pose of the ligand, giving the binding pocket greater flexibility and accordingly allowing for an induced fit of the enzyme-ligand binding site around the newly introduced ligand. The change in the loop2 of ligand-bound Bc II may lead to the opening of the binding pocket of Bc II. Therefore, loop2 can be considered a gate for control of ligand access in Bc II, hence its dynamic response should be considered in new drug design and development.
[Mh] Termos MeSH primário: Bacillus cereus/metabolismo
Cefalosporinase/metabolismo
Penicilina V/metabolismo
Sulbactam/metabolismo
[Mh] Termos MeSH secundário: Sítios de Ligação/fisiologia
Simulação de Dinâmica Molecular
Ligação Proteica/fisiologia
Conformação Proteica em Folha beta
Análise Espectral/métodos
Eletricidade Estática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.5.2.- (Cephalosporinase); S4TF6I2330 (Sulbactam); Z61I075U2W (Penicillin V)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE


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[PMID]:27655293
[Au] Autor:Sadeghi MR; Ghotaslou R; Akhi MT; Asgharzadeh M; Hasani A
[Ad] Endereço:1​Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2​Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
[Ti] Título:Molecular characterization of extended-spectrum ß-lactamase, plasmid-mediated AmpC cephalosporinase and carbapenemase genes among Enterobacteriaceae isolates in five medical centres of East and West Azerbaijan, Iran.
[So] Source:J Med Microbiol;65(11):1322-1331, 2016 Nov.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Very little is known about the occurrence and various types of extended-spectrum ß-lactamase (ESBL), AmpC and carbapenemase in Iran. The aims of this study were to determine the prevalence of ESBLs, AmpCs and carbapenemase genes among Enterobacteriaceae in Azerbaijan and to characterize the genetic composition of the detected genes. A total of 307 Enterobacteriaceae isolates, recovered from five medical centres, were screened for ESBL, AmpC and carbapenemase activities by the disc diffusion method and phenotypic confirmatory tests. The 162 selected strains (third-generation cephalosporins, cefoxitin- or carbapenem-resistant strains with positive or negative phenotypic confirmatory tests) were selected for multiplex PCR screening for ß-lactamase genes, and detected genes were confirmed by sequencing. Of 162 isolates, 156 harboured 1 to 6 ß-lactamase genes of 41 types. The most prevalent genes were blaTEM-1 (29.9 %), followed by blaCTX-M-15 (25.7 %). Plasmid-mediated AmpC was detected in 66 strains (21.5 %) alone or in combination with other genes. Carbapenemase-encoding genes were detected in 18 strains (5.8 %) of 27 carbapenem-non-susceptible isolates including 11, 7, 3 and 1 cases of blaOXA-48, blaNDM-1, blaKPC-2 and blaKPC-3 genes, respectively. Interestingly, 148 (94.8 %) of 156 strains with any ß-lactamase gene were found to have a multidrug-resistant pattern. The rate of resistance to ß-lactams and multidrug-resistant Enterobacteriaceae is high in Azerbaijan. All positive strains for carbapenemase genes were resistant to all ß-lactams. The present study reveals the high occurrence of CTX-M-type ESBLs followed by TEM and SHV variants among Enterobacteriaceae isolates. East Azerbaijan seems to be an alarming focus for OXA-48, NDM-1 and KPC dissemination.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Cefalosporinase/genética
Infecções por Enterobacteriaceae/microbiologia
Enterobacteriaceae/enzimologia
Enterobacteriaceae/isolamento & purificação
Plasmídeos/genética
beta-Lactamases/genética
[Mh] Termos MeSH secundário: Centros Médicos Acadêmicos
Amoxicilina/farmacologia
Antibacterianos/farmacologia
Proteínas de Bactérias/metabolismo
Cefalosporinase/metabolismo
Enterobacteriaceae/efeitos dos fármacos
Enterobacteriaceae/genética
Seres Humanos
Irã (Geográfico)
Plasmídeos/metabolismo
beta-Lactamases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 804826J2HU (Amoxicillin); EC 3.5.2.- (Cephalosporinase); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (carbapenemase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160923
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000356


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[PMID]:27572403
[Au] Autor:Liakopoulos A; Olsen B; Geurts Y; Artursson K; Berg C; Mevius DJ; Bonnedahl J
[Ad] Endereço:Department of Bacteriology and Epidemiology, CVI of Wageningen University, Lelystad, the Netherlands apostolos.liakopoulos@wur.nl.
[Ti] Título:Molecular Characterization of Extended-Spectrum-Cephalosporin-Resistant Enterobacteriaceae from Wild Kelp Gulls in South America.
[So] Source:Antimicrob Agents Chemother;60(11):6924-6927, 2016 Nov.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Extended-spectrum-cephalosporin-resistant Enterobacteriaceae are a public health concern due to limited treatment options. Here, we report on the occurrence and the molecular characteristics of extended-spectrum-cephalosporin-resistant Enterobacteriaceae recovered from wild birds (kelp gulls). Our results revealed kelp gulls as a reservoir of various extended-spectrum cephalosporinase genes associated with different genetic platforms. In addition, we report for the first time the presence of a known epidemic clone of Salmonella enterica serotype Heidelberg (JF6X01.0326/XbaI.1966) among wild birds.
[Mh] Termos MeSH primário: Resistência às Cefalosporinas/genética
Charadriiformes/microbiologia
Enterobacteriaceae/efeitos dos fármacos
Enterobacteriaceae/genética
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Resistência às Cefalosporinas/efeitos dos fármacos
Cefalosporinase/genética
Cefalosporinas/farmacologia
Enterobacteriaceae/isolamento & purificação
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Escherichia coli/isolamento & purificação
Plasmídeos/genética
Salmonella enterica/efeitos dos fármacos
Salmonella enterica/genética
América do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephalosporins); EC 3.5.2.- (Cephalosporinase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160831
[St] Status:MEDLINE
[do] DOI:10.1128/AAC.01120-16


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[PMID]:27528799
[Au] Autor:Hidalgo JA; Vinluan CM; Antony N
[Ad] Endereço:UTEP/UT Austin Cooperative Pharmacy Program, College of Health Sciences, University of Texas at El Paso, El Paso; Department of Pharmacy, College of Pharmacy, The University of Texas at Austin, Austin.
[Ti] Título:Ceftazidime/avibactam: a novel cephalosporin/nonbeta-lactam beta-lactamase inhibitor for the treatment of complicated urinary tract infections and complicated intra-abdominal infections.
[So] Source:Drug Des Devel Ther;10:2379-86, 2016.
[Is] ISSN:1177-8881
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:There has been greater interest in developing additional antimicrobial agents due to the increasing health care costs and resistance resulting from bacterial pathogens to currently available treatment options. Gram-negative organisms including Enterobacteriaceae and Pseudomonas aeruginosa are some of the most concerning threats due to their resistance mechanisms: extended-spectrum beta-lactamase production and Klebsiella pneumoniae carbapenemase enzymes. Ceftazidime is a third-generation broad-spectrum cephalosporin with activity against P. aeruginosa and avibactam is a novel nonbeta-lactam beta-lactamase inhibitor. Avycaz(®), the trade name for this new combination antibiotic, restores the activity of ceftazidime against some of the previously resistant pathogens. Avycaz was approved in 2015 for the treatment of complicated urinary tract infections, including pyelonephritis, and complicated intra-abdominal infections with the addition of metronidazole in patients with little to no other treatment options. This review article assesses the clinical trials and data that led to the approval of this antibiotic, in addition to its spectrum of activity and limitations.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Compostos Azabicíclicos/uso terapêutico
Ceftazidima/uso terapêutico
Infecções Intra-Abdominais/tratamento farmacológico
Infecções Urinárias/tratamento farmacológico
Inibidores de beta-Lactamases/uso terapêutico
[Mh] Termos MeSH secundário: Antibacterianos/administração & dosagem
Antibacterianos/farmacologia
Compostos Azabicíclicos/administração & dosagem
Compostos Azabicíclicos/farmacologia
Ceftazidima/administração & dosagem
Ceftazidima/farmacologia
Cefalosporinase/metabolismo
Seres Humanos
Infecções Intra-Abdominais/complicações
Infecções Intra-Abdominais/microbiologia
Klebsiella pneumoniae/efeitos dos fármacos
Infecções Urinárias/complicações
Infecções Urinárias/microbiologia
Inibidores de beta-Lactamases/administração & dosagem
Inibidores de beta-Lactamases/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Azabicyclo Compounds); 0 (beta-Lactamase Inhibitors); 7352665165 (avibactam); 9M416Z9QNR (Ceftazidime); EC 3.5.2.- (Cephalosporinase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160817
[St] Status:MEDLINE
[do] DOI:10.2147/DDDT.S110946


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[PMID]:26869096
[Au] Autor:Lalak A; Wasyl D; Zajac M; Skarzynska M; Hoszowski A; Samcik I; Wozniakowski G; Szulowski K
[Ad] Endereço:Department of Microbiology, National Veterinary Research Institute, Pulawy, Poland.
[Ti] Título:Mechanisms of cephalosporin resistance in indicator Escherichia coli isolated from food animals.
[So] Source:Vet Microbiol;194:69-73, 2016 Oct 15.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Resistance to ß-lactams is considered one of the major global problems and recently it became the most frequently studied topic in the area of antimicrobial resistance. The study was focused on phenotypic and genetic characterisation of commensal Escherichia coli (E. coli), including those producing cephalosporinases, isolated from gut flora of healthy slaughter animals. E. coli were cultured simultaneously on MacConkey agar (MCA) and cefotaxime supplemented MCA. The isolates were confirmed with ONPG and indol tube tests as well as PCR targeting uspA gene. Microbroth dilution method was applied for determination of Minimal Inhibitory Concentrations and interpreted according to EUCAST epidemiological cut-off values. Cephalosporin resistance phenotypes were defined by E-tests (BioMerieux) and relevant gene amplicons from selected strains were sequenced. A total of 298 E. coli isolates with cephalosporin resistance (ESC) found in 99 ones, were obtained from 318 cloacal or rectal swabs deriving from broilers, layers, turkeys, pigs and cattle. Both extended spectrum ß-lactamase (ESBL) and ampC-cephalosporinase resistance phenotypes were noted in all tested animal species but cattle. At least one of the analysed genes was identified in 90 out of 99 cephalosporin-resistant isolates: bla (n=44), bla (n=38), bla (n=33) and bla (n=12). None of the phenotypes was identified in nine isolates. Sequencing of PCR products showed occurrence of ESBL-genes: bla , bla , bla and ampC-gene bla . They were located on numerous and diverse plasmids and resistance transferability was proved by electroporation of bla and bla located on X1 plasmids. Detection of cephalosporin resistant E. coli confirms the existence of resistance genes reservoir in farm animals and their possible spread (i.e. via IncX1 plasmids) to other bacteria including human and animal pathogens. The identified genetic background indicates on ecological aspects of selection and dissemination of cephalosporin resistance in E. coli isolated from food-producing animals rather than its potential role for public health threats.
[Mh] Termos MeSH primário: Resistência às Cefalosporinas/genética
Farmacorresistência Bacteriana/genética
Escherichia coli/efeitos dos fármacos
Escherichia coli/fisiologia
Microbiologia de Alimentos
[Mh] Termos MeSH secundário: Animais
Bovinos
Cefalosporinase/genética
Galinhas
Escherichia coli/enzimologia
Escherichia coli/genética
Intestinos/microbiologia
Suínos
Perus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.5.2.- (Cephalosporinase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170131
[Lr] Data última revisão:
170131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160213
[St] Status:MEDLINE


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[PMID]:26718943
[Au] Autor:Hennequin C; Robin F
[Ad] Endereço:Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France.
[Ti] Título:Correlation between antimicrobial resistance and virulence in Klebsiella pneumoniae.
[So] Source:Eur J Clin Microbiol Infect Dis;35(3):333-41, 2016 Mar.
[Is] ISSN:1435-4373
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Klebsiella pneumoniae is responsible for a wide range of infections, including urinary tract infections, pneumonia, bacteremia, and liver abscesses. In addition to susceptible clinical isolates involved in nosocomial infections, multidrug-resistant (MDR) and hypervirulent (hvKP) strains have evolved separately in distinct clonal groups. The rapid geographic spread of these isolates is of particular concern. However, we still know little about the virulence of K. pneumoniae except for hvKP, whose secrets are beginning to be revealed. The treatment of K. pneumoniae infections is threatened by the emergence of antimicrobial resistance. The dissemination of resistance is associated with genetic mobile elements, such as plasmids that may also carry virulence determinants. A proficient pathogen should be virulent, resistant to antibiotics, and epidemic. However, the interplay between resistance and virulence is poorly understood. Here, we review current knowledge on the topic.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Farmacorresistência Bacteriana
Infecções por Klebsiella/microbiologia
Klebsiella pneumoniae/efeitos dos fármacos
Klebsiella pneumoniae/patogenicidade
[Mh] Termos MeSH secundário: Anti-Infecciosos/uso terapêutico
Proteínas da Membrana Bacteriana Externa/genética
Proteínas da Membrana Bacteriana Externa/metabolismo
Cefalosporinase/genética
Cefalosporinase/metabolismo
Farmacorresistência Bacteriana Múltipla/genética
Expressão Gênica
Genoma Bacteriano
Estudo de Associação Genômica Ampla
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Infecções por Klebsiella/tratamento farmacológico
Klebsiella pneumoniae/genética
Klebsiella pneumoniae/metabolismo
Plasmídeos/genética
Virulência/genética
Resistência beta-Lactâmica
beta-Lactamases/genética
beta-Lactamas/farmacologia
beta-Lactamas/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Bacterial Outer Membrane Proteins); 0 (beta-Lactams); EC 3.5.2.- (Cephalosporinase); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160101
[St] Status:MEDLINE
[do] DOI:10.1007/s10096-015-2559-7


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[PMID]:26073861
[Au] Autor:Powers RA
[Ad] Endereço:Department of Chemistry, Grand Valley State University 1 Campus Drive, Allendale, MI 49401, USA. powersra@gvsu.edu.
[Ti] Título:Structural and Functional Aspects of Extended-Spectrum AmpC Cephalosporinases.
[So] Source:Curr Drug Targets;17(9):1051-60, 2016.
[Is] ISSN:1873-5592
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:ß-lactam antibiotics have revolutionized modern medicine, but resistance to these drugs is a major public health crisis. Traditionally, class C ß-lactamases were referred to as cephalosporinases due to their substrate preference for this particular class of ß-lactams. However, the emergence of AmpC enzymes with extended-spectrum activity (extended-spectrum cephalosporinases or ESACs) is particularly worrisome, especially given that most clinical ß-lactamase inhibitors are ineffective against these enzymes. This review summarizes structures of several extended spectrum class C ß-lactamases and analyzes the structure-function relationship observed among them.
[Mh] Termos MeSH primário: Cefalosporinase/química
Cefalosporinase/metabolismo
Inibidores de beta-Lactamases/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Sítios de Ligação
Desenho de Drogas
Seres Humanos
Ligantes
Modelos Moleculares
Conformação Proteica
Resistência beta-Lactâmica
Inibidores de beta-Lactamases/farmacologia
beta-Lactamases/química
beta-Lactamases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Ligands); 0 (beta-Lactamase Inhibitors); EC 3.5.2.- (Cephalosporinase); EC 3.5.2.6 (AmpC beta-lactamases); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150616
[St] Status:MEDLINE


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[PMID]:26676375
[Au] Autor:Cavalcanti FL; Mirones CR; Paucar ER; Montes LÁ; Leal-Balbino TC; Morais MM; Martínez-Martínez L; Ocampo-Sosa AA
[Ad] Endereço:Laboratório de Resistência Microbiana, Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, PE, Brasil.
[Ti] Título:Mutational and acquired carbapenem resistance mechanisms in multidrug resistant Pseudomonas aeruginosa clinical isolates from Recife, Brazil.
[So] Source:Mem Inst Oswaldo Cruz;110(8):1003-9, 2015 Dec.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:An investigation was carried out into the genetic mechanisms responsible for multidrug resistance in nine carbapenem-resistant Pseudomonas aeruginosa isolates from different hospitals in Recife, Brazil. Susceptibility to antimicrobial agents was determined by broth microdilution. Polymerase chain reaction (PCR) was employed to detect the presence of genes encoding ß-lactamases, aminoglycoside-modifying enzymes (AMEs), 16S rRNA methylases, integron-related genes and OprD. Expression of genes coding for efflux pumps and AmpC cephalosporinase were assessed by quantitative PCR. The outer membrane proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The blaSPM-1, blaKPC-2 and blaGES-1 genes were detected in P. aeruginosa isolates in addition to different AME genes. The loss of OprD in nine isolates was mainly due to frameshift mutations, premature stop codons and point mutations. An association of loss of OprD with the overexpression of MexAB-OprM and MexXY-OprM was observed in most isolates. Hyper-production of AmpC was also observed in three isolates. Clonal relationship of the isolates was determined by repetitive element palindromic-PCR and multilocus sequence typing. Our results show that the loss of OprD along with overexpression of efflux pumps and ß-lactamase production were responsible for the multidrug resistance in the isolates analysed.
[Mh] Termos MeSH primário: Carbapenêmicos/metabolismo
Farmacorresistência Bacteriana Múltipla/genética
Mutação
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/genética
Resistência beta-Lactâmica/genética
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Aminoglicosídeos/metabolismo
Anfotericina B/análogos & derivados
Anfotericina B/metabolismo
Antifúngicos/metabolismo
Brasil
Cefalosporinase/classificação
Cefalosporinase/metabolismo
Códon sem Sentido/metabolismo
Ativação Enzimática/genética
Mutação da Fase de Leitura/genética
Regulação Bacteriana da Expressão Gênica/genética
Seres Humanos
Proteínas de Membrana Transportadoras/metabolismo
Metiltransferases/metabolismo
Nucleotidiltransferases/metabolismo
Mutação Puntual/genética
Porinas/metabolismo
Pseudomonas aeruginosa/enzimologia
Pseudomonas aeruginosa/isolamento & purificação
Sequências Repetitivas de Ácido Nucleico
beta-Lactamases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aminoglycosides); 0 (Antifungal Agents); 0 (Carbapenems); 0 (Codon, Nonsense); 0 (Membrane Transport Proteins); 0 (Porins); 074Z98YIW3 (methylamphotericin B); 148412-32-2 (OprD protein, Pseudomonas aeruginosa); 7XU7A7DROE (Amphotericin B); EC 2.1.1.- (Methyltransferases); EC 2.1.1.182 (16S rRNA (adenine(1518)-N(6)-adenine(1519)-N(6))-dimethyltransferase); EC 2.7.7.- (Nucleotidyltransferases); EC 3.5.2.- (Cephalosporinase); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151218
[St] Status:MEDLINE


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[PMID]:26666930
[Au] Autor:Voulgari E; Poulou A; Dimitroulia E; Politi L; Ranellou K; Gennimata V; Markou F; Pournaras S; Tsakris A
[Ad] Endereço:Department of Microbiology, Medical School, University of Athens, Athens, Greece.
[Ti] Título:Emergence of OXA-162 Carbapenemase- and DHA-1 AmpC Cephalosporinase-Producing Sequence Type 11 Klebsiella pneumoniae Causing Community-Onset Infection in Greece.
[So] Source:Antimicrob Agents Chemother;60(3):1862-4, 2015 Dec 14.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OXA-48-like carbapenemases have only recently emerged in Europe. OXA-162 is a rare OXA-48 variant usually coexpressed with extended-spectrum ß-lactamases. Here, we report the identification of the first OXA-162 carbapenemase-producing Klebsiella pneumoniae isolates, which coexpressed an AmpC cephalosporinase (DHA-1), retrieved from a patient in Greece. They belonged to a single sequence type (ST11) and caused the first documented community-onset urinary tract infections attributable to an OXA-48-like-producing Enterobacteriaceae strain.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Cefalosporinase/genética
Infecções por Klebsiella/tratamento farmacológico
Klebsiella pneumoniae/efeitos dos fármacos
Infecções Urinárias/tratamento farmacológico
beta-Lactamases/genética
[Mh] Termos MeSH secundário: Infecções Comunitárias Adquiridas/tratamento farmacológico
Infecções Comunitárias Adquiridas/microbiologia
Farmacorresistência Bacteriana Múltipla/genética
Feminino
Grécia
Seres Humanos
Infecções por Klebsiella/microbiologia
Klebsiella pneumoniae/isolamento & purificação
Testes de Sensibilidade Microbiana
Meia-Idade
Tipagem de Sequências Multilocus
Infecções Urinárias/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); EC 3.5.2.- (Cephalosporinase); EC 3.5.2.6 (AmpC beta-lactamases); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase DHA-1, Klebsiella pneumoniae); EC 3.5.2.6 (carbapenemase); EC 3.5.2.6 (oxacillinase)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151216
[St] Status:MEDLINE
[do] DOI:10.1128/AAC.01514-15



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