[PMID]: | 28655305 |
[Au] Autor: | Amri Z; Ghorbel A; Turki M; Akrout FM; Ayadi F; Elfeki A; Hammami M |
[Ad] Endereço: | Biochemistry Laboratory, LR12ES05 "Nutrition- Functional Foods and vascular Health", Faculty of Medicine, University of Monastir, 5019, Monastir, Tunisia. zahraamri23@yahoo.fr. |
[Ti] Título: | Effect of pomegranate extracts on brain antioxidant markers and cholinesterase activity in high fat-high fructose diet induced obesity in rat model. |
[So] Source: | BMC Complement Altern Med;17(1):339, 2017 Jun 27. |
[Is] ISSN: | 1472-6882 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | BACKGROUND: To investigate beneficial effects of Pomegranate seeds oil (PSO), leaves (PL), juice (PJ) and (PP) on brain cholinesterase activity, brain oxidative stress and lipid profile in high-fat-high fructose diet (HFD) induced-obese rat. METHODS: In vitro and in vivo cholinesterase activity, brain oxidative status, body and brain weight and plasma lipid profile were measured in control rats, HFD-fed rats and HFD-fed rats treated by PSO, PL, PJ and PP. RESULTS: In vitro study showed that PSO, PL, PP, PJ inhibited cholinesterase activity in dose dependant manner. PL extract displayed the highest inhibitory activity by IC50 of 151.85 mg/ml. For in vivo study, HFD regime induced a significant increase of cholinesterase activity in brain by 17.4% as compared to normal rats. However, the administration of PSO, PL, PJ and PP to HDF-rats decreased cholinesterase activity in brain respectively by 15.48%, 6.4%, 20% and 18.7% as compared to untreated HFD-rats. Moreover, HFD regime caused significant increase in brain stress, brain and body weight, and lipid profile disorders in blood. Furthermore, PSO, PL, PJ and PP modulated lipid profile in blood and prevented accumulation of lipid in brain and body evidenced by the decrease of their weights as compared to untreated HFD-rats. In addition administration of these extract protected brain from stress oxidant, evidenced by the decrease of malondialdehyde (MDA) and Protein carbonylation (PC) levels and the increase in superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels. CONCLUSION: These findings highlight the neuroprotective effects of pomegranate extracts and one of mechanisms is the inhibition of cholinesterase and the stimulation of antioxidant capacity. |
[Mh] Termos MeSH primário: |
Antioxidantes/metabolismo Encéfalo/efeitos dos fármacos Colinesterases/metabolismo Dieta Hiperlipídica/efeitos adversos Frutose/efeitos adversos Obesidade/tratamento farmacológico Extratos Vegetais/administração & dosagem Punicaceae/química
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[Mh] Termos MeSH secundário: |
Animais Encéfalo/enzimologia Encéfalo/metabolismo Modelos Animais de Doenças Glutationa Peroxidase/metabolismo Seres Humanos Masculino Malondialdeído/metabolismo Obesidade/enzimologia Obesidade/metabolismo Estresse Oxidativo/efeitos dos fármacos Ratos Ratos Wistar Superóxido Dismutase/metabolismo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antioxidants); 0 (Plant Extracts); 30237-26-4 (Fructose); 4Y8F71G49Q (Malondialdehyde); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); EC 3.1.1.8 (Cholinesterases) |
[Em] Mês de entrada: | 1708 |
[Cu] Atualização por classe: | 170817 |
[Lr] Data última revisão:
| 170817 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170629 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1186/s12906-017-1842-9 |
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