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  1 / 11706 MEDLINE  
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[PMID]:27964890
[Au] Autor:Piel MS; Peters GH; Brask J
[Ad] Endereço:Novozymes A/S, Krogshøjvej 36, 2880 Bagsværd, Denmark.
[Ti] Título:Chemoenzymatic synthesis of fluorogenic phospholipids and evaluation in assays of phospholipases A, C and D.
[So] Source:Chem Phys Lipids;202:49-54, 2017 Jan.
[Is] ISSN:1873-2941
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Phospholipases are ubiquitous in nature and the target of significant research aiming at both their physiological roles and technical applications in e.g. the food industry. In the search for sensitive and selective phospholipase assays, we have focused on synthetic FRET (Förster resonance energy transfer) substrates. This has led to the development of a facile, easily scalable and low cost synthesis of fluorogenic phospholipids featuring the dansyl/dabcyl fluorophore/quencher-pair on the fatty acid ω-position and on the phosphatidylethanolamine head group, respectively. Hence, the two substrates lyso-(dansyl-FA)-GPE-dabcyl (6) and (dansyl-FA) -GPE-dabcyl (7) were synthesized by a chemoenzymatic strategy, in which preparation of (6) further included a novel selective enzymatic esterification step. As proof of concept, activity of a handful of phospholipases, one from each of the PLA1, PLA2, PLC and PLD classes, were assayed using substrates (6) and (7), and the kinetic parameter k /K was determined. The PLA1 (Lecitase Ultra™) was found to be highly active on both substrates, whereas the PLD (from white cabbage) had no activity, presumably due to steric effects associated with the dabcyl-functionalization of the head group. It was further substantiated that the substrates are specific towards phospholipase activity as the tested lipase (Lipolase™) showed close to zero activity.
[Mh] Termos MeSH primário: Ensaios Enzimáticos
Transferência Ressonante de Energia de Fluorescência
Corantes Fluorescentes/metabolismo
Fosfolipase D/metabolismo
Fosfolipases A/metabolismo
Fosfolipídeos/biossíntese
Fosfolipídeos/síntese química
Fosfolipases Tipo C/metabolismo
[Mh] Termos MeSH secundário: Corantes Fluorescentes/química
Cinética
Estrutura Molecular
Fosfolipase D/química
Fosfolipases A/química
Fosfolipídeos/metabolismo
Fosfolipases Tipo C/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Phospholipids); EC 3.1.1.32 (Phospholipases A); EC 3.1.4.- (Type C Phospholipases); EC 3.1.4.4 (Phospholipase D)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161215
[St] Status:MEDLINE


  2 / 11706 MEDLINE  
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[PMID]:26366855
[Au] Autor:Selb J; Kogovsek R; Silar M; Kosnik M; Korosec P
[Ad] Endereço:University Clinic of Respiratory and Allergic Diseases Golnik, Golnik, Slovenia.
[Ti] Título:Improved recombinant Api m 1- and Ves v 5-based IgE testing to dissect bee and yellow jacket allergy and their correlation with the severity of the sting reaction.
[So] Source:Clin Exp Allergy;46(4):621-30, 2016 Apr.
[Is] ISSN:1365-2222
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: No study has assessed the diagnostic sensitivity of rApi m 1 and rVes v 5 on Immulite testing system. OBJECTIVE: To compare the diagnostic sensitivity of commercially available venom recombinant allergens between the currently available immunoassays [ImmunoCAP (CAP) and Immulite (LITE)] and establish their correlation with the severity of the sting reaction. METHODS: This study evaluated 95 bee venom and 110 yellow jacket venom-allergic subjects. We measured the levels of sIgE to rApi m 1, rVes v 5 (LITE and CAP), rApi m 2 (LITE), rVes v 1 (CAP) and total IgE (CAP). Forty-nine healthy subjects served as controls. RESULTS: The diagnostic sensitivity of rApi m 1 and rVes v 5 was significantly higher with the LITE than with the CAP system (71% vs. 88% and 82% vs. 93%). The specificity of both assays for both allergens was between 94% and 98%. Twenty-nine patients that tested negative for rApi m 1 or rVes v 5 with CAP were positive with LITE, but none of the patients that tested negative with LITE were positive with CAP. The positive values of rApi m 1 and rVes v 5 were on average 2.7 and 2.3 times higher, with the LITE than with the CAP system. The combination of rApi m 1 and rApi m 2 (LITE) and the combination of rVes v 5 (LITE) and rVes v 1 (CAP) almost matched the sensitivity of native venoms (95% and 97%, respectively), whereas the diagnostic sensitivity of the combination of rVes v 5 and rVes v 1 (CAP) did not reach the sensitivity of rVes v 5 (LITE) alone (90% vs. 93%). IgE levels to venom recombinants and total IgE did not correlate with the severity of sting reaction. CONCLUSIONS & CLINICAL RELEVANCE: The use of rApi m 1 and rVes v 5 with the LITE system significantly enhanced diagnostic utility of venom recombinants and should improve the dissection of bee and yellow jacket venom allergy.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Abelhas
Hipersensibilidade/diagnóstico
Hipersensibilidade/imunologia
Imunoglobulina E/imunologia
Mordeduras e Picadas de Insetos
Vespas
[Mh] Termos MeSH secundário: Alérgenos/administração & dosagem
Animais
Venenos de Abelha/imunologia
Feminino
Seres Humanos
Imunoglobulina E/sangue
Proteínas de Insetos/imunologia
Masculino
Fosfolipases A/imunologia
Kit de Reagentes para Diagnóstico
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Índice de Gravidade de Doença
Venenos de Vespas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Bee Venoms); 0 (Insect Proteins); 0 (Reagent Kits, Diagnostic); 0 (Ves v 5 allergen); 0 (Wasp Venoms); 37341-29-0 (Immunoglobulin E); EC 3.1.1.32 (Phospholipases A); EC 3.1.1.4 (Api m 1 allergen, Apis mellifera)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150915
[St] Status:MEDLINE
[do] DOI:10.1111/cea.12639


  3 / 11706 MEDLINE  
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[PMID]:26457430
[Au] Autor:Salvador GH; Dreyer TR; Cavalcante WL; Matioli FF; Dos Santos JI; Velazquez-Campoy A; Gallacci M; Fontes MR
[Ad] Endereço:Department of Physics and Biophysics, Institute of Biosciences, UNESP - Universidade Estadual Paulista, Botucatu-SP, Brazil.
[Ti] Título:Structural and functional evidence for membrane docking and disruption sites on phospholipase A2-like proteins revealed by complexation with the inhibitor suramin.
[So] Source:Acta Crystallogr D Biol Crystallogr;71(Pt 10):2066-78, 2015 Oct.
[Is] ISSN:1399-0047
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Local myonecrosis resulting from snakebite envenomation is not efficiently neutralized by regular antivenom administration. This limitation is considered to be a significant health problem by the World Health Organization. Phospholipase A2-like (PLA2-like) proteins are among the most important proteins related to the muscle damage resulting from several snake venoms. However, despite their conserved tertiary structure compared with PLA2s, their biological mechanism remains incompletely understood. Different oligomeric conformations and binding sites have been identified or proposed, leading to contradictory data in the literature. In the last few years, a comprehensive hypothesis has been proposed based on fatty-acid binding, allosteric changes and the presence of two different interaction sites. In the present study, a combination of techniques were used to fully understand the structural-functional characteristics of the interaction between suramin and MjTX-II (a PLA2-like toxin). In vitro neuromuscular studies were performed to characterize the biological effects of the protein-ligand interaction and demonstrated that suramin neutralizes the myotoxic activity of MjTX-II. The high-resolution structure of the complex identified the toxin-ligand interaction sites. Calorimetric assays showed two different binding events between the protein and the inhibitor. It is demonstrated for the first time that the inhibitor binds to the surface of the toxin, obstructing the sites involved in membrane docking and disruption according to the proposed myotoxic mechanism. Furthermore, higher-order oligomeric formation by interaction with interfacial suramins was observed, which may also aid the inhibitory process. These results further substantiate the current myotoxic mechanism and shed light on the search for efficient inhibitors of the local myonecrosis phenomenon.
[Mh] Termos MeSH primário: Antivenenos/farmacologia
Bothrops/metabolismo
Venenos de Crotalídeos/antagonistas & inibidores
Venenos de Crotalídeos/metabolismo
Fosfolipases A/antagonistas & inibidores
Fosfolipases A/metabolismo
Suramina/farmacologia
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação
Membrana Celular/efeitos dos fármacos
Membrana Celular/metabolismo
Venenos de Crotalídeos/química
Venenos de Crotalídeos/toxicidade
Cristalografia por Raios X
Masculino
Camundongos
Simulação de Acoplamento Molecular
Simulação de Dinâmica Molecular
Fosfolipases A/química
Fosfolipases A/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antivenins); 0 (Crotalid Venoms); 6032D45BEM (Suramin); EC 3.1.1.- (myotoxin II, Bothrops moojeni); EC 3.1.1.32 (Phospholipases A)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151013
[St] Status:MEDLINE
[do] DOI:10.1107/S1399004715014443


  4 / 11706 MEDLINE  
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[PMID]:26290597
[Au] Autor:Liu G; Zhang K; Ai J; Deng X; Hong Y; Wang X
[Ad] Endereço:National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China.
[Ti] Título:Patatin-related phospholipase A, pPLAIIIα, modulates the longitudinal growth of vegetative tissues and seeds in rice.
[So] Source:J Exp Bot;66(21):6945-55, 2015 Nov.
[Is] ISSN:1460-2431
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Patatin-related phospholipase A (pPLA) hydrolyses glycerolipids to produce fatty acids and lysoglycerolipids. The Oryza sativa genome has 21 putative pPLAs that are grouped into five subfamilies. Overexpression of OspPLAIIIα resulted in a dwarf phenotype with decreased length of rice stems, roots, leaves, seeds, panicles, and seeds, whereas OspPLAIIIα-knockout plants had longer panicles and seeds. OspPLAIIIα-overexpressing plants were less sensitive than wild-type and knockout plants to gibberellin-promoted seedling elongation. OspPLAIIIα overexpression and knockout had an opposite effect on the expression of the growth repressor SLENDER1 in the gibberellin signalling process. OspPLAIIIα-overexpressing plants had decreased mechanical strength and cellulose content, but exhibited increases in the expression of several cellulose synthase genes. These results indicate that OspPLAIIIα plays a role in rice vegetative and reproductive growth and that the constitutive, high activity of OspPLAIIIα suppresses cell elongation. The decreased gibberellin response in overexpressing plants is probably a result of the decreased ability to make cellulose for anisotropic cell expansion.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas
Oryza/crescimento & desenvolvimento
Oryza/genética
Fosfolipases A/genética
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Regulação da Expressão Gênica no Desenvolvimento
Giberelinas/metabolismo
Oryza/metabolismo
Fosfolipases A/química
Fosfolipases A/metabolismo
Filogenia
Reguladores de Crescimento de Planta/metabolismo
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Gibberellins); 0 (Plant Growth Regulators); 0 (Plant Proteins); EC 3.1.1.32 (Phospholipases A)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150821
[St] Status:MEDLINE
[do] DOI:10.1093/jxb/erv402


  5 / 11706 MEDLINE  
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[PMID]:26148472
[Au] Autor:Jeong JW; Lee WS; Go SI; Nagappan A; Baek JY; Lee JD; Lee SJ; Park C; Kim GY; Kim HJ; Kim GS; Kwon TK; Ryu CH; Shin SC; Choi YH
[Ad] Endereço:Departments of Biochemistry, Dongeui University College of Korean Medicine, Busan, 614-052, Republic of Korea.
[Ti] Título:Pachymic Acid Induces Apoptosis of EJ Bladder Cancer Cells by DR5 Up-Regulation, ROS Generation, Modulation of Bcl-2 and IAP Family Members.
[So] Source:Phytother Res;29(10):1516-24, 2015 Oct.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pachymic acid (PA) is a lanostane-type triterpenoid derived from Poria cocos mushroom that possess various biological effects such as anti-cancer, antiinflammatory and anti-metastasis effects. In this study, we investigated the anti-cancer effects of PA in EJ bladder cancer cells. The results showed that PA significantly inhibited proliferation of EJ cells in a dose-dependent manner. PA induced accumulation of sub-G1 DNA content (apoptotic cell population), apoptotic bodies and chromatin condensation and DNA fragmentation in EJ cells in a dose-dependent manner. PA also induces activation of caspase-3, -8 and -9, and subsequent cleavage of poly (ADP-ribose) polymerase, and significantly suppressed the inhibitor of apoptosis protein family proteins in a dose-dependent manner. Furthermore, PA activates Bid and induced the loss of mitochondrial membrane potential (ΔΨm ) with up-regulated pro-apoptotic proteins (Bax and Bad), down-regulated anti-apoptotic proteins (Bcl-2 and Bcl-xL) and cytochrome c release. In turn, PA increased the generation of reactive oxygen species (ROS); also, the ROS production was blocked by N-acetyl-L-cysteine. The expressions of TNF-related apoptosis inducing ligand and death receptor 5 were up-regulated by PA in a dose-dependent manner, suggesting extrinsic pathway also involved in PA-induced apoptosis. This study provides evidence that PA might be useful in the treatment of human bladder cancer.
[Mh] Termos MeSH primário: Espécies Reativas de Oxigênio
Receptores do Ligante Indutor de Apoptose Relacionado a TNF
Triterpenos/farmacologia
Proteína X Associada a bcl-2
[Mh] Termos MeSH secundário: Acetilcisteína/farmacologia
Apoptose/efeitos dos fármacos
Proteínas Reguladoras de Apoptose/metabolismo
Caspase 3/metabolismo
Caspases/metabolismo
Citocromos c/metabolismo
Fragmentação do DNA
Regulação para Baixo
Seres Humanos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Fosfolipases A/antagonistas & inibidores
Poli(ADP-Ribose) Polimerases/metabolismo
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Receptores do Ligante Indutor de Apoptose Relacionado a TNF/metabolismo
Ligante Indutor de Apoptose Relacionado a TNF
Regulação para Cima
Neoplasias da Bexiga Urinária
Proteína X Associada a bcl-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (Reactive Oxygen Species); 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand); 0 (TNF-Related Apoptosis-Inducing Ligand); 0 (TNFSF10 protein, human); 0 (Triterpenes); 0 (bcl-2-Associated X Protein); 9007-43-6 (Cytochromes c); EC 2.4.2.30 (Poly(ADP-ribose) Polymerases); EC 3.1.1.32 (Phospholipases A); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspases); WYQ7N0BPYC (Acetylcysteine); X2FCK16QAH (pachymic acid)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150708
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5402


  6 / 11706 MEDLINE  
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[PMID]:25736542
[Au] Autor:Ruiz B; Serrano P; Verdú M; Moreno C
[Ad] Endereço:Department of Allergology, Hospital General La Mancha Centro, Alcázar de San Juan, Ciudad Real, Spain. Electronic address: rulebe@gmail.com.
[Ti] Título:Sensitization to Api m 1, Api m 2, and Api m 4: association with safety of bee venom immunotherapy.
[So] Source:Ann Allergy Asthma Immunol;114(4):350-2, 2015 Apr.
[Is] ISSN:1534-4436
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Alérgenos/imunologia
Venenos de Abelha/uso terapêutico
Dessensibilização Imunológica/métodos
Hialuronoglucosaminidase/imunologia
Hipersensibilidade/terapia
Proteínas de Insetos/imunologia
Meliteno/imunologia
Fosfolipases A/imunologia
[Mh] Termos MeSH secundário: Adulto
Animais
Abelhas/imunologia
Biomarcadores/metabolismo
Progressão da Doença
Feminino
Seres Humanos
Hipersensibilidade/imunologia
Imunização
Imunoglobulina E/sangue
Masculino
Meia-Idade
Adulto Jovem
[Pt] Tipo de publicação:LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Allergens); 0 (Bee Venoms); 0 (Biomarkers); 0 (Insect Proteins); 20449-79-0 (Melitten); 37341-29-0 (Immunoglobulin E); EC 3.1.1.32 (Phospholipases A); EC 3.1.1.4 (Api m 1 allergen, Apis mellifera); EC 3.2.1.35 (Api m 2 allergen, Apis mellifera); EC 3.2.1.35 (Hyaluronoglucosaminidase)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150305
[St] Status:MEDLINE


  7 / 11706 MEDLINE  
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[PMID]:25557877
[Au] Autor:Li M; Wei F; Tawfall A; Tang M; Saettele A; Wang X
[Ad] Endereço:Department of Biology, University of Missouri, St. Louis, MO, USA.
[Ti] Título:Overexpression of patatin-related phospholipase AIIIδ altered plant growth and increased seed oil content in camelina.
[So] Source:Plant Biotechnol J;13(6):766-78, 2015 Aug.
[Is] ISSN:1467-7652
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Camelina sativa is a Brassicaceae oilseed species being explored as a biofuel and industrial oil crop. A growing number of studies have indicated that the turnover of phosphatidylcholine plays an important role in the synthesis and modification of triacylglycerols. This study manipulated the expression of a patatin-related phospholipase AIIIδ (pPLAIIIδ) in camelina to determine its effect on seed oil content and plant growth. Constitutive overexpression of pPLAIIIδ under the control of the constitutive cauliflower mosaic 35S promoter resulted in a significant increase in seed oil content and a decrease in cellulose content. In addition, the content of major membrane phospholipids, phosphatidylcholine and phosphatidylethanolamine, in 35S::pPLAIIIδ plants was increased. However, these changes in 35S::pPLAIIIδ camelina were associated with shorter cell length, leaves, stems, and seed pods and a decrease in overall seed production. When pPLAIIIδ was expressed under the control of the seed specific, ß-conglycinin promoter, the seed oil content was increased without compromising plant growth. The results suggest that pPLAIIIδ alters the carbon partitioning by decreasing cellulose content and increasing oil content in camelina.
[Mh] Termos MeSH primário: Brassicaceae/crescimento & desenvolvimento
Fosfolipases A/metabolismo
Óleos Vegetais/metabolismo
Sementes/metabolismo
[Mh] Termos MeSH secundário: Brassicaceae/enzimologia
Brassicaceae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Plant Oils); EC 3.1.1.32 (Phospholipases A)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150106
[St] Status:MEDLINE
[do] DOI:10.1111/pbi.12304


  8 / 11706 MEDLINE  
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[PMID]:25533115
[Au] Autor:Ferri N; Corsini A
[Ti] Título:[Role of secreted and lipoprotein-associated phospholipase A2 in cardiovascular risk].
[Ti] Título:Ruolo della fosfolipasi A2 secretoria e della fosfolipasi associata alle lipoproteine nel rischio cardiovascolare..
[So] Source:G Ital Cardiol (Rome);15(12):664-9, 2014 Dec.
[Is] ISSN:1827-6806
[Cp] País de publicação:Italy
[La] Idioma:ita
[Ab] Resumo:Phospholipase A(2) (PLA(2)) are enzymes that hydrolyze the ester bond of glycerophospholipids releasing free fatty acids and lysophospholipids, including the arachidonic acid, the precursor of the eicosanoids and the inflammatory cascades. PLA(2) are present in the atherosclerotic plaques and their direct involvement in the proatherogenic inflammatory response is well documented. Epidemiological and genetic studies have demonstrated the correlation of the PLA(2) mass and enzymatic activity with the incidence of cardiovascular diseases. The potential pro-atherogenic role of PLA(2) led to the development of two small molecules, varespladib, a reversible sPLA(2) inhibitor, and darapladib, a selective Lp-PLA(2) inhibitor. Both molecules have demonstrated antiatherosclerotic properties in animal models, and positive effects on atherosclerotic plaque composition evaluated in phase 2 clinical trials. On these grounds, the results of three phase 3 studies have recently been published: the VISTA-16 study with varespladib in patients with acute coronary syndrome, and the STABILITY and SOLID-TIMI 52 studies with darapladib in patients with stable coronary heart disease and acute coronary syndrome, respectively. Unexpectedly, both studies did not demonstrate an additional protective action of PLA 2 inhibitors over the standard of care treatment with statins, antiplatelet drugs, and coronary revascularization. In the present article, the enzymatic properties and the involvement of sPLA(2) and Lp-PLA(2) in atherogenesis are reviewed, with a focus on the results of experimental studies and clinical studies with both varespladib and darapladib inhibitors.
[Mh] Termos MeSH primário: 1-Alquil-2-acetilglicerofosfocolina Esterase/antagonistas & inibidores
1-Alquil-2-acetilglicerofosfocolina Esterase/fisiologia
Aterosclerose/etiologia
Fosfolipases A/antagonistas & inibidores
Fosfolipases A/fisiologia
[Mh] Termos MeSH secundário: Acetatos/farmacologia
Síndrome Coronariana Aguda/tratamento farmacológico
Aterosclerose/enzimologia
Benzaldeídos/farmacologia
Ensaios Clínicos Fase III como Assunto
Doença da Artéria Coronariana/tratamento farmacológico
Seres Humanos
Indóis/farmacologia
Lipoproteínas/fisiologia
Oximas/farmacologia
Inibidores de Fosfolipase A2/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Acetates); 0 (Benzaldehydes); 0 (Indoles); 0 (Lipoproteins); 0 (Oximes); 0 (Phospholipase A2 Inhibitors); 2Q3P98DATH (varespladib); EC 3.1.1.32 (Phospholipases A); EC 3.1.1.47 (1-Alkyl-2-acetylglycerophosphocholine Esterase); UI1U1MYH09 (darapladib)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141224
[St] Status:MEDLINE
[do] DOI:10.1714/1718.18766


  9 / 11706 MEDLINE  
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[PMID]:25428555
[Au] Autor:Dong Y; Li M; Zhang P; Wang X; Fan C; Zhou Y
[Ti] Título:Patatin-related phospholipase pPLAIIIδ influences auxin-responsive cell morphology and organ size in Arabidopsis and Brassica napus.
[So] Source:BMC Plant Biol;14:332, 2014 Nov 27.
[Is] ISSN:1471-2229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The members of the patatin-related phospholipase subfamily III (pPLAIIIs) have been implicated in the auxin response. However, it is not clear whether and how these genes affect plant and cell morphogenesis. Here, we studied the roles of the patatin-related phospholipase pPLAIIIδ in auxin-responsive cell morphology and organ size in Arabidopsis and Brassica napus. RESULTS: We show that overexpression of pPLAIIIδ inhibited longitudinal growth but promoted transverse growth in most organs of Arabidopsis and Brassica napus. Compared to wild-type plants, pPLAIIIδ-KO plants exhibited enhanced cell elongation in hypocotyls, and pPLAIIIδ-OE plants displayed broadened radial cell growth of hypocotyl and reduced leaf pavement cell polarity. For the hypocotyl phenotype in pPLAIIIδ mutants, which resembles the "triple response" to ethylene, we examined the expression of the ACS and ACO genes involved in ethylene biosynthesis and found that ACS4 and ACS5 were up-regulated by 2.5-fold on average in two OE lines compared with WT plants. The endogenous auxin distribution was disturbed in plants with altered pPLAIIIδ expression. pPLAIIIδ-OE and KO plants exhibited different sensitivities to indole-3-acetic acid-promoted hypocotyl elongation in both light and dark conditions. Gene expression analysis of auxin-induced genes in the dark showed that OE plants maintained a higher auxin response compared with WT and KO plants after treatment with 1 µM IAA for 12 h. Following treatment with 10 µM IAA for 30 min in the light, early auxin-induced genes were significantly up-regulated in two OE plant lines. CONCLUSIONS: These data suggest that the PLAIIIδ gene plays an important role in cell morphology and organ size through its involvement in the regulation of auxin distribution in plants.
[Mh] Termos MeSH primário: Arabidopsis/enzimologia
Brassica napus/enzimologia
Regulação da Expressão Gênica de Plantas
Ácidos Indolacéticos/metabolismo
Fosfolipases A/metabolismo
Reguladores de Crescimento de Planta/metabolismo
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Brassica napus/genética
Brassica napus/crescimento & desenvolvimento
Crescimento Celular
Mutação
Fosfolipases A/genética
Proteínas de Plantas/genética
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Indoleacetic Acids); 0 (Plant Growth Regulators); 0 (Plant Proteins); EC 3.1.1.- (pPLAIIIdelta protein, Arabidopsis); EC 3.1.1.32 (Phospholipases A)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141128
[St] Status:MEDLINE
[do] DOI:10.1186/s12870-014-0332-1


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[PMID]:25266374
[Au] Autor:El Alaoui M; Noiriel A; Soulère L; Grand L; Queneau Y; Abousalham A
[Ad] Endereço:Institut de Chimie et de Biochimie Moléculaires et Supramoléculaires (ICBMS) UMR 5246 CNRS, Organisation et Dynamique des Membranes Biologiques, Université Lyon 1 , Bâtiment Raulin, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
[Ti] Título:Development of a high-throughput assay for measuring phospholipase A activity using synthetic 1,2-α-eleostearoyl-sn-glycero-3-phosphocholine coated on microtiter plates.
[So] Source:Anal Chem;86(21):10576-83, 2014 Nov 04.
[Is] ISSN:1520-6882
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To date, several sensitive methods, based on radiolabeled elements or sterically hindered fluorochrome groups, are usually employed to screen phospholipase A (PLA) activities. With the aim of developing a convenient, specific, sensitive, and continuous new ultraviolet (UV) spectrophotometric assay for PLA, we have synthesized a specific glycerophosphatidylcholine (PC) esterified at the sn-1 and sn-2 positions, with α-eleostearic acid (9Z, 11E, 13E-octadecatrienoic acid) purified from Aleurites fordii seed oil. The conjugated triene present in α-eleostearic acid constitutes an intrinsic chromophore and, consequently, confers the strong UV absorption properties of this free fatty acid as well as of the glycerophospholipids harboring it. This coated PC film cannot be desorbed by the various buffers used during PLA assays. Following the action of PLA at the oil-water interface, α-eleostearic acid is freed and desorbed from the film and then solubilized with ß-cyclodextrin. The UV absorbance of the α-eleostearic acid is considerably enhanced due to the transformation from an adsorbed to a water-soluble state. The PLA activity can be measured continuously by recording the variations with time of the UV absorption spectra. The rate of lipolysis was monitored by measuring the increase of absorption at 272 nm, which was found to be linear with time and proportional to the amount of added PLA. This continuous high-throughput PLA assay could be used to screen new PLA and/or PLA inhibitors present in various biological samples.
[Mh] Termos MeSH primário: Ascomicetos/enzimologia
Abelhas/enzimologia
Ensaios Enzimáticos/métodos
Ácidos Linolênicos/química
Fosfatidilcolinas/química
Fosfolipases A/metabolismo
[Mh] Termos MeSH secundário: Aleurites/química
Animais
Ensaios de Triagem em Larga Escala/métodos
Ácidos Linolênicos/metabolismo
Fosfatidilcolinas/metabolismo
Fosfolipases A/análise
Óleos Vegetais/química
Espectrofotometria Ultravioleta/métodos
beta-Ciclodextrinas/química
beta-Ciclodextrinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Linolenic Acids); 0 (Phosphatidylcholines); 0 (Plant Oils); 0 (beta-Cyclodextrins); 13296-76-9 (eleostearic acid); EC 3.1.1.32 (Phospholipases A); JV039JZZ3A (betadex)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141001
[St] Status:MEDLINE
[do] DOI:10.1021/ac502096v



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