Base de dados : MEDLINE Pesquisa : D08.811.277.352.100.680.750.875 [Categoria DeCS] Referências encontradas : 398 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 28796890 [Au] Autor: Uyama T; Tsuboi K; Ueda N [Ad] Endereço: Department of Biochemistry, Kagawa University School of Medicine, Japan. [Ti] Título: An involvement of phospholipase A/acyltransferase family proteins in peroxisome regulation and plasmalogen metabolism. [So] Source: FEBS Lett;591(18):2745-2760, 2017 Sep. [Is] ISSN: 1873-3468 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The H-Ras-like suppressor (HRASLS) is a protein family consisting of five members in humans. Despite their discovery as tumor suppressors, we demonstrated that all these proteins are phospholipid-metabolizing enzymes, such as phospholipase (PL) A /A and acyltransferase. We thus proposed to rename HRASLS1-5 as PLA/acyltransferase (PLAAT)-1-5. Notably, PLAATs exhibit N-acyltransferase activity to biosynthesize N-acylated ethanolamine phospholipids, including N-acyl-plasmalogen, which serve as precursors of bioactive N-acylethanolamines. Furthermore, the overexpression of PLAAT-3 in animal cells causes disappearance of peroxisomes and a remarkable reduction in plasmalogen levels. This finding might be related to the inhibitory effect of PLAAT-3 on the chaperone activity of the peroxin PEX19. In this article, we will review our recent findings about PLAAT proteins, with special reference to their roles in peroxisome biogenesis and plasmalogen metabolism. [Mh] Termos MeSH primário: Peroxissomos/metabolismo Plasmalogênios/metabolismo [Mh] Termos MeSH secundário: Animais Diacilglicerol O-Aciltransferase/metabolismo Etanolaminas/metabolismo Seres Humanos Proteínas de Membrana/metabolismo Fosfolipases A1/metabolismo Fosfolipases A2/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Ethanolamines); 0 (Membrane Proteins); 0 (N-acylethanolamines); 0 (Plasmalogens); EC 2.3.1.20 (Diacylglycerol O-Acyltransferase); EC 3.1.1.32 (Phospholipases A1); EC 3.1.1.4 (Phospholipases A2) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171011 [Lr] Data última revisão: 171011 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170811 [St] Status: MEDLINE [do] DOI: 10.1002/1873-3468.12787

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[PMID]: 28793214 [Au] Autor: Marx DC; Fleming KG [Ad] Endereço: Johns Hopkins University, Baltimore, Maryland. [Ti] Título: Influence of Protein Scaffold on Side-Chain Transfer Free Energies. [So] Source: Biophys J;113(3):597-604, 2017 Aug 08. [Is] ISSN: 1542-0086 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The process by which membrane proteins fold involves the burial of side chains into lipid bilayers. Both structure and function of membrane proteins depend on the magnitudes of side-chain transfer free energies (ΔΔG ). In the absence of other interactions, ΔΔG is an independent property describing the energetics of an isolated side chain in the bilayer. However, in reality, side chains are attached to the peptide backbone and surrounded by other side chains in the protein scaffold in biology, which may alter the apparent ΔΔG . Previously we reported a whole protein water-to-bilayer hydrophobicity scale using the transmembrane ß-barrel Escherichia coli OmpLA as a scaffold protein. To investigate how a different protein scaffold can modulate these energies, we measured ΔΔG for all 20 amino acids using the transmembrane ß-barrel E. coli PagP as a scaffold protein. This study represents, to our knowledge, the first instance of ΔΔG measured in the same experimental conditions in two structurally and sequentially distinct protein scaffolds. Although the two hydrophobicity scales are strongly linearly correlated, we find that there are apparent scaffold induced changes in ΔΔG for more than half of the side chains, most of which are polar residues. We propose that the protein scaffold affects the ΔΔG of side chains that are buried in unfavorable environments by dictating the mechanisms by which the side chain can reach a more favorable environment and thus modulating the magnitude of ΔΔG . [Mh] Termos MeSH primário: Proteínas da Membrana Bacteriana Externa/química Proteínas da Membrana Bacteriana Externa/metabolismo Fosfolipases A1/química Fosfolipases A1/metabolismo [Mh] Termos MeSH secundário: Membrana Celular/metabolismo Interações Hidrofóbicas e Hidrofílicas Simulação de Dinâmica Molecular Conformação Proteica em Folha beta Termodinâmica [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bacterial Outer Membrane Proteins); EC 3.1.1.32 (Phospholipases A1); EC 3.1.1.32 (outer membrane phospholipase A) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170831 [Lr] Data última revisão: 170831 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170810 [St] Status: MEDLINE

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[PMID]: 28283404 [Au] Autor: Rangl M; Rima L; Klement J; Miyagi A; Keller S; Scheuring S [Ad] Endereço: Department of Anesthesiology, Physiology and Biophysics, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA; INSERM U1006, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 Avenue de Luminy, 13009 Marseille, France. [Ti] Título: Real-time Visualization of Phospholipid Degradation by Outer Membrane Phospholipase A using High-Speed Atomic Force Microscopy. [So] Source: J Mol Biol;429(7):977-986, 2017 Apr 07. [Is] ISSN: 1089-8638 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Phospholipases are abundant in various types of cells and compartments, where they play key roles in physiological processes as diverse as digestion, cell proliferation, and neural activation. In Gram-negative bacteria, outer membrane phospholipase A (OmpLA) is involved in outer-membrane lipid homeostasis and bacterial virulence. Although the enzymatic activity of OmpLA can be probed with an assay relying on an artificial monoacyl thioester substrate, only little is known about its activity on diacyl phospholipids. Here, we used high-speed atomic force microscopy (HS-AFM) to directly image enzymatic phospholipid degradation by OmpLA in real time. In the absence of Ca , reconstituted OmpLA diffused within a phospholipid bilayer without revealing any signs of phospholipase activity. Upon the addition of Ca , OmpLA was activated and degraded the membrane with a turnover of ~2 phospholipid molecules per second and per OmpLA dimer until most of the membrane phospholipids were hydrolyzed and the protein became tightly packed. [Mh] Termos MeSH primário: Proteínas da Membrana Bacteriana Externa/metabolismo Microscopia de Força Atômica/métodos Fosfolipases A1/metabolismo Fosfolipídeos/metabolismo [Mh] Termos MeSH secundário: Cálcio/metabolismo Cinética Modelos Biológicos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bacterial Outer Membrane Proteins); 0 (Phospholipids); EC 3.1.1.32 (Phospholipases A1); EC 3.1.1.32 (outer membrane phospholipase A); SY7Q814VUP (Calcium) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170703 [Lr] Data última revisão: 170703 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170312 [St] Status: MEDLINE

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[PMID]: 28143894 [Au] Autor: Emoto S; Kurano M; Kano K; Matsusaki K; Yamashita H; Nishikawa M; Igarashi K; Ikeda H; Aoki J; Kitayama J; Yatomi Y [Ad] Endereço: Department of Surgical Oncology, University of Tokyo, Tokyo, Japan. [Ti] Título: Analysis of glycero-lysophospholipids in gastric cancerous ascites. [So] Source: J Lipid Res;58(4):763-771, 2017 Apr. [Is] ISSN: 1539-7262 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Lysophosphatidic acid (LysoPA) has been proposed to be involved in the pathogenesis of various cancers. Moreover, glycero-lysophospholipids (glycero-LysoPLs) other than LysoPA are now emerging as novel lipid mediators. Therefore, we aimed to elucidate the possible involvement of glycero-LysoPLs in the pathogenesis of gastric cancer by measuring glycero-LysoPLs, autotaxin (ATX), and phosphatidylserine-specific phospholipase A1 (PS-PLA ) in ascites obtained from patients with gastric cancer and those with cirrhosis (as a control). We observed that after adjustments according to the albumin levels, the lysophosphatidylserine (LysoPS) and lysophosphatidylglycerol (LysoPG) levels were significantly higher, while the LysoPA and ATX levels were lower, in the ascites from patients with gastric cancer. We also found that multiple regression analyses revealed that ATX was selected as a significant explanatory factor for all the detectable LysoPA species only in the cirrhosis group and that a significant positive correlation was observed between LysoPS and PS-PLA only in the gastric cancer group. In conclusion, the LysoPA levels might be determined largely by LysoPC and LysoPI (possible precursors) and the PS-PLA -mediated pathway might be involved in the production of LysoPS in gastric cancer. Glycero-LysoPLs other than LysoPA might also be involved in the pathogenesis of cancer directly or through being converted into LysoPA. [Mh] Termos MeSH primário: Lisofosfolipídeos/metabolismo Fosfolipases A1/metabolismo Neoplasias Gástricas/metabolismo [Mh] Termos MeSH secundário: Animais Ascite/metabolismo Ascite/patologia Feminino Fibrose/metabolismo Fibrose/patologia Seres Humanos Lisofosfolipídeos/isolamento & purificação Masculino Camundongos Fosfolipases A1/genética Diester Fosfórico Hidrolases Neoplasias Gástricas/patologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Lysophospholipids); 0 (lysophosphatidylglycerol); 0 (lysophosphatidylserine); EC 3.1.1.32 (PLA1A protein, human); EC 3.1.1.32 (Phospholipases A1); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 3.1.4.39 (alkylglycerophosphoethanolamine phosphodiesterase); PG6M3969SG (lysophosphatidic acid) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170906 [Lr] Data última revisão: 170906 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170202 [St] Status: MEDLINE [do] DOI: 10.1194/jlr.P072090

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[PMID]: 28064428 [Au] Autor: Chen W; Guo W; Gao F; Chen L; Chen S; Li D [Ad] Endereço: Tianjin Key Laboratory for Industrial Biological Systems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesTianjin, No. 32, Xiqi Road, Tianjin Airport Economic Park, Tianjin, 300308, People's Republic of China. [Ti] Título: Phospholipase A1-Catalysed Synthesis of Docosahexaenoic Acid-Enriched Phosphatidylcholine in Reverse Micelles System. [So] Source: Appl Biochem Biotechnol;182(3):1037-1052, 2017 Jul. [Is] ISSN: 1559-0291 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Phosphatidylcholine enriched with docosahexaenoic acid (DHA) was successfully produced by phospholipase A1-catalysed acidolysis. Reverse micelles were firstly selected as the reaction system to avoid the process of immobilization and to ensure the efficient catalysis of phospholipase A1. Parameters were optimized for a high incorporation of phosphatidylcholine enriched with DHA (DHA-PC). A response-surface design with four factors, including the water content, enzyme loading, pH and substrate-mass ratio were used to evaluate the influence of the major factors and to predict the optimal reaction conditions. The results indicated that the optimal reaction conditions for the production of DHA-PC were a water content of 0.4%, an enzyme loading of 40%, pH 6.92 and a substrate-mass ratio of 2.13. Under these optimized conditions, 20.90% of DHA content in DHA-PC was obtained. [Mh] Termos MeSH primário: Ácidos Docosa-Hexaenoicos/síntese química Micelas Fosfatidilcolinas/química Fosfolipases A1/química [Mh] Termos MeSH secundário: Catálise Ácidos Docosa-Hexaenoicos/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Micelles); 0 (Phosphatidylcholines); 25167-62-8 (Docosahexaenoic Acids); EC 3.1.1.32 (Phospholipases A1) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170626 [Lr] Data última revisão: 170626 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170109 [St] Status: MEDLINE [do] DOI: 10.1007/s12010-016-2379-y

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[PMID]: 28007846 [Au] Autor: Kurano M; Kano K; Dohi T; Matsumoto H; Igarashi K; Nishikawa M; Ohkawa R; Ikeda H; Miyauchi K; Daida H; Aoki J; Yatomi Y [Ad] Endereço: Department of Clinical Laboratory Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan. [Ti] Título: Different origins of lysophospholipid mediators between coronary and peripheral arteries in acute coronary syndrome. [So] Source: J Lipid Res;58(2):433-442, 2017 Feb. [Is] ISSN: 1539-7262 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Lysophosphatidic acids (LysoPAs) and lysophosphatidylserine (LysoPS) are emerging lipid mediators proposed to be involved in the pathogenesis of acute coronary syndrome (ACS). In this study, we attempted to elucidate how LysoPA and LysoPS become elevated in ACS using human blood samples collected simultaneously from culprit coronary arteries and peripheral arteries in ACS subjects. We found that: 1) the plasma LysoPA, LysoPS, and lysophosphatidylglycerol levels were not different, while the lysophosphatidylcholine (LysoPC), lysophosphatidylinositol, and lysophosphatidylethanolamine (LysoPE) levels were significantly lower in the culprit coronary arteries; 2) the serum autotaxin (ATX) level was lower and the serum phosphatidylserine-specific phospholipase A (PS-PLA ) level was higher in the culprit coronary arteries; 3) the LysoPE and ATX levels were significant explanatory factors for the mainly elevated species of LysoPA, except for 22:6 LysoPA, in the peripheral arteries, while the LysoPC and LysoPE levels, but not the ATX level, were explanatory factors in the culprit coronary arteries; and 4) 18:0 and 18:1 LysoPS were significantly correlated with PS-PLA only in the culprit coronary arteries. In conclusion, the origins of LysoPA and LysoPS might differ between culprit coronary arteries and peripheral arteries, and substrates for ATX, such as LysoPC and LysoPE, might be important for the generation of LysoPA in ACS. [Mh] Termos MeSH primário: Síndrome Coronariana Aguda/sangue Aterosclerose/sangue Vasos Coronários/metabolismo Lisofosfolipídeos/sangue [Mh] Termos MeSH secundário: Síndrome Coronariana Aguda/patologia Aterosclerose/metabolismo Aterosclerose/patologia Vasos Coronários/patologia Feminino Coração/fisiopatologia Seres Humanos Masculino Espectrometria de Massas Fosfolipases A1/sangue Diester Fosfórico Hidrolases/sangue [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Lysophospholipids); 0 (lysophosphatidylglycerol); 0 (lysophosphatidylinositol); 0 (lysophosphatidylserine); EC 3.1.1.32 (Phospholipases A1); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 3.1.4.39 (alkylglycerophosphoethanolamine phosphodiesterase) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170825 [Lr] Data última revisão: 170825 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161224 [St] Status: MEDLINE [do] DOI: 10.1194/jlr.P071803

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[PMID]: 27821336 [Au] Autor: Xin R; Khan FI; Zhao Z; Zhang Z; Yang B; Wang Y [Ad] Endereço: College of Food Sciences and Engineering, South China University of Technology, Guangzhou 510640, PR China. [Ti] Título: A comparative study on kinetics and substrate specificities of Phospholipase A with Thermomyces lanuginosus lipase. [So] Source: J Colloid Interface Sci;488:149-154, 2017 Feb 15. [Is] ISSN: 1095-7103 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The mechanism of lipase binding to the lipid-water interface is crucial for substrate specificity and kinetic properties. In this study, the chain-length specificity, regiospecificity and substrate specificity of Phospholipase A (PLA ) and its parent enzyme Thermomyces lanuginosus lipase (TLL) have been investigated using a classical emulsion system. The results show that both PLA and TLL are 1,3-regioselective lipases. Additionally, the hydrolytic activity of PLA is comparatively lower on short-chain triacylglyceride (TAG) and higher on phosphatidylcholine (PC) than the hydrolytic activity of TLL. Further, the results obtained with monolayer film techniques demonstrate that the C-terminal region regulates the binding of PLA to PC. A hypothesis is presented according to which the α9 helix of C-terminal region in PLA not only controls the opening of lid but also serves as a membrane anchor that assists in binding to PC. These findings bring new insight into rational design of novel lipases with intriguing functionalities. [Mh] Termos MeSH primário: Proteínas Fúngicas/química Lipase/química Fosfatidilcolinas/química Fosfolipases A1/química Triglicerídeos/química [Mh] Termos MeSH secundário: Sequência de Aminoácidos Emulsões Ensaios Enzimáticos Proteínas Fúngicas/genética Proteínas Fúngicas/metabolismo Expressão Gênica Cinética Lipase/genética Lipase/metabolismo Fosfatidilcolinas/metabolismo Fosfolipases A1/genética Fosfolipases A1/metabolismo Conformação Proteica em alfa-Hélice Proteínas Recombinantes/química Proteínas Recombinantes/genética Proteínas Recombinantes/metabolismo Saccharomycetales/química Saccharomycetales/enzimologia Alinhamento de Sequência Homologia de Sequência de Aminoácidos Estereoisomerismo Especificidade por Substrato Triglicerídeos/metabolismo [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Emulsions); 0 (Fungal Proteins); 0 (Phosphatidylcholines); 0 (Recombinant Proteins); 0 (Triglycerides); EC 3.1.1.3 (Lipase); EC 3.1.1.32 (Phospholipases A1) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170407 [Lr] Data última revisão: 170407 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161109 [St] Status: MEDLINE

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[PMID]: 27719893 [Au] Autor: Xie M; Dunford NT [Ad] Endereço: School of Food Equipment Engineering and Science, Xi'an Jiaotong University, Xi'an 710049, China; Robert M. Kerr Food and Agricultural Products Center, Oklahoma State University, Stillwater, OK 74078, USA. [Ti] Título: Lipid composition and emulsifying properties of canola lecithin from enzymatic degumming. [So] Source: Food Chem;218:159-164, 2017 Mar 01. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: This study investigated the polar lipid composition and emulsifying properties of canola lecithin from enzymatic degumming (CLED). Phospholipase A was used for enzymatic degumming of crude canola oil to collect lecithin sample. Canola lecithin from water degumming (CLWD) was also collected and served as the control. The results showed that the contents of phosphatidylethanolamine (PE) (2.99%) and phosphatidylcholine (PC) (6.59%) in CLED were significantly lower than that in CLWD (PE 15.55% and PC 21.93%); while the content of lysophosphatidylcholine (LPC) (19.45%) in CLED was significantly higher than that in CLWD (3.27%). Unsaturated fatty acids accounted for a higher percentage of the total fatty acids in CLED than in CLWD. CLED promoted more stable o/w emulsions than CLWD. This study provides a better understanding of the chemical nature of CLED, and important information for utilization of CLED as o/w emulsifier. [Mh] Termos MeSH primário: Emulsificantes/química Ácidos Graxos Monoinsaturados/química Ácidos Graxos/química Lecitinas/química Lipídeos/química Fosfolipases A1/metabolismo [Mh] Termos MeSH secundário: Óleo de Canola Emulsões Fosfatidilcolinas/química Fosfatidiletanolaminas/química Água/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Canola Oil); 0 (Emulsifying Agents); 0 (Emulsions); 0 (Fatty Acids); 0 (Fatty Acids, Monounsaturated); 0 (Lecithins); 0 (Lipids); 0 (Phosphatidylcholines); 0 (Phosphatidylethanolamines); 059QF0KO0R (Water); 39382-08-6 (phosphatidylethanolamine); EC 3.1.1.32 (Phospholipases A1) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161011 [St] Status: MEDLINE

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[PMID]: 27826019 [Au] Autor: Perez-Riverol A; Campos Pereira FD; Musacchio Lasa A; Romani Fernandes LG; Santos-Pinto JR; Justo-Jacomini DL; Oliveira de Azevedo G; Bazon ML; Palma MS; Zollner RL; Brochetto-Braga MR [Ad] Endereço: Laboratório de Biologia Molecular de Artrópodes-LBMA-IBRC-UNESP (Univ Estadual Paulista), Av. 24-A, nº 1515, CEP 13506-900, Bela Vista, Rio Claro, SP, Brazil. Electronic address: aperezriverol@gmail.com. [Ti] Título: Molecular cloning, expression and IgE-immunoreactivity of phospholipase A1, a major allergen from Polybia paulista (Hymenoptera: Vespidae) venom. [So] Source: Toxicon;124:44-52, 2016 Dec 15. [Is] ISSN: 1879-3150 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Polybia paulista (Hymenoptera: Vespidae) is a clinically relevant social wasp that frequently causes stinging accidents in southeast Brazil. To date, diagnosis and specific immunotherapy (SIT) of allergy are based on the use of crude venom extracts. Production of recombinant forms of major allergens from P. paulista venom will improve diagnosis and SIT of allergic patients by reducing the incidence of cross-reactivity and non-specific sensitization. Here, we describe the molecular cloning, heterologous expression, purification and IgE-mediated immunodetection of phospholipase A1 (Poly p 1), a major allergen from P. paulista venom. The cDNA of Poly p 1 was extracted from venom glands and then cloned, and further expression of the recombinant allergen (rPoly p 1) was achieved in Escherichia coli BL21 (DE3) cells. Purification of rPoly p 1 was performed using immobilized Ni metal affinity chromatography. Also, a single-step chromatographic method allowed the purification of native Poly p 1 (nPoly p 1) from the wasp's venom glands. We used western blotting to evaluate IgE-reactivity of the sera from 10 P. paulista venom-allergic patients to rPoly p 1 and nPoly p 1. High levels of insoluble rPoly p 1 were obtained during heterologous expression. After solubilization of inclusion bodies and purification of the recombinant protein, a unique band of ∼34 kDa was detected in SDS-PAGE analysis. Allergen-specific IgE (sIgE) from allergic patients' sera recognized rPoly p 1, nPoly p 1 and crude venom extract to a similar extent. Our results showed that rPoly p 1 could be used for development of component-resolved diagnosis (CRD) and molecular-defined SIT of P. paulista venom allergy. [Mh] Termos MeSH primário: Alérgenos/imunologia Imunoglobulina E/imunologia Fosfolipases A1/imunologia Venenos de Vespas/imunologia [Mh] Termos MeSH secundário: Alérgenos/química Alérgenos/genética Alérgenos/isolamento & purificação Sequência de Aminoácidos Animais Sequência de Bases Clonagem Molecular DNA Complementar Eletroforese em Gel de Poliacrilamida Seres Humanos Fosfolipases A1/química Fosfolipases A1/genética Fosfolipases A1/isolamento & purificação Homologia de Sequência de Aminoácidos Vespas [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Allergens); 0 (DNA, Complementary); 0 (Wasp Venoms); 37341-29-0 (Immunoglobulin E); EC 3.1.1.32 (Phospholipases A1) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170411 [Lr] Data última revisão: 170411 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161110 [St] Status: MEDLINE

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[PMID]: 27746179 [Au] Autor: Urafuji K; Arioka M [Ad] Endereço: Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. [Ti] Título: Yor022c protein is a phospholipase A that localizes to the mitochondrial matrix. [So] Source: Biochem Biophys Res Commun;480(3):302-308, 2016 Nov 18. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: In mammals, three types of intracellular phospholipase A (iPLA ) enzymes have been characterized and are thought to be involved in various cellular processes such as phospholipid metabolism, organelle biogenesis, and membrane trafficking. In this study we analyzed the unique iPLA -like protein, Yor022c, in the budding yeast Saccharomyces cerevisiae. By the mass spectrometry analysis, we demonstrate that Yor022c is actually a phospholipase displaying sn-1-specific activity toward phosphatidylcholine, phosphatidylethanolamine, and phosphatidic acid, generating 2-acyl lysophospholipids. GFP-fused Yor022c co-stained with the mitochondrial dye MitoTracker, indicating that, unlike its mammalian counterparts, it is a mitochondrial protein. Further biochemical fractionation experiment combined with protease sensitivity assay showed that Yor022c localizes to the mitochondrial matrix. Thus Yor022c is the first PLA putatively involved in the maintenance of sn-1 acyl chains of phospholipids in the mitochondrial inner membrane. [Mh] Termos MeSH primário: Fosfolipases A1/metabolismo Proteínas de Saccharomyces cerevisiae/metabolismo Saccharomyces cerevisiae/metabolismo [Mh] Termos MeSH secundário: Ativação Enzimática Mitocôndrias Saccharomyces cerevisiae/ultraestrutura Distribuição Tecidual [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Saccharomyces cerevisiae Proteins); EC 3.1.1.32 (Phospholipases A1); EC 3.1.1.32 (Yor022c protein, S cerevisiae) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170529 [Lr] Data última revisão: 170529 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161106 [St] Status: MEDLINE

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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