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Pesquisa : D08.811.277.352.100.680.750.875 [Categoria DeCS]
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[PMID]:28796890
[Au] Autor:Uyama T; Tsuboi K; Ueda N
[Ad] Endereço:Department of Biochemistry, Kagawa University School of Medicine, Japan.
[Ti] Título:An involvement of phospholipase A/acyltransferase family proteins in peroxisome regulation and plasmalogen metabolism.
[So] Source:FEBS Lett;591(18):2745-2760, 2017 Sep.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The H-Ras-like suppressor (HRASLS) is a protein family consisting of five members in humans. Despite their discovery as tumor suppressors, we demonstrated that all these proteins are phospholipid-metabolizing enzymes, such as phospholipase (PL) A /A and acyltransferase. We thus proposed to rename HRASLS1-5 as PLA/acyltransferase (PLAAT)-1-5. Notably, PLAATs exhibit N-acyltransferase activity to biosynthesize N-acylated ethanolamine phospholipids, including N-acyl-plasmalogen, which serve as precursors of bioactive N-acylethanolamines. Furthermore, the overexpression of PLAAT-3 in animal cells causes disappearance of peroxisomes and a remarkable reduction in plasmalogen levels. This finding might be related to the inhibitory effect of PLAAT-3 on the chaperone activity of the peroxin PEX19. In this article, we will review our recent findings about PLAAT proteins, with special reference to their roles in peroxisome biogenesis and plasmalogen metabolism.
[Mh] Termos MeSH primário: Peroxissomos/metabolismo
Plasmalogênios/metabolismo
[Mh] Termos MeSH secundário: Animais
Diacilglicerol O-Aciltransferase/metabolismo
Etanolaminas/metabolismo
Seres Humanos
Proteínas de Membrana/metabolismo
Fosfolipases A1/metabolismo
Fosfolipases A2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Ethanolamines); 0 (Membrane Proteins); 0 (N-acylethanolamines); 0 (Plasmalogens); EC 2.3.1.20 (Diacylglycerol O-Acyltransferase); EC 3.1.1.32 (Phospholipases A1); EC 3.1.1.4 (Phospholipases A2)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12787


  2 / 398 MEDLINE  
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[PMID]:28793214
[Au] Autor:Marx DC; Fleming KG
[Ad] Endereço:Johns Hopkins University, Baltimore, Maryland.
[Ti] Título:Influence of Protein Scaffold on Side-Chain Transfer Free Energies.
[So] Source:Biophys J;113(3):597-604, 2017 Aug 08.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The process by which membrane proteins fold involves the burial of side chains into lipid bilayers. Both structure and function of membrane proteins depend on the magnitudes of side-chain transfer free energies (ΔΔG ). In the absence of other interactions, ΔΔG is an independent property describing the energetics of an isolated side chain in the bilayer. However, in reality, side chains are attached to the peptide backbone and surrounded by other side chains in the protein scaffold in biology, which may alter the apparent ΔΔG . Previously we reported a whole protein water-to-bilayer hydrophobicity scale using the transmembrane ß-barrel Escherichia coli OmpLA as a scaffold protein. To investigate how a different protein scaffold can modulate these energies, we measured ΔΔG for all 20 amino acids using the transmembrane ß-barrel E. coli PagP as a scaffold protein. This study represents, to our knowledge, the first instance of ΔΔG measured in the same experimental conditions in two structurally and sequentially distinct protein scaffolds. Although the two hydrophobicity scales are strongly linearly correlated, we find that there are apparent scaffold induced changes in ΔΔG for more than half of the side chains, most of which are polar residues. We propose that the protein scaffold affects the ΔΔG of side chains that are buried in unfavorable environments by dictating the mechanisms by which the side chain can reach a more favorable environment and thus modulating the magnitude of ΔΔG .
[Mh] Termos MeSH primário: Proteínas da Membrana Bacteriana Externa/química
Proteínas da Membrana Bacteriana Externa/metabolismo
Fosfolipases A1/química
Fosfolipases A1/metabolismo
[Mh] Termos MeSH secundário: Membrana Celular/metabolismo
Interações Hidrofóbicas e Hidrofílicas
Simulação de Dinâmica Molecular
Conformação Proteica em Folha beta
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); EC 3.1.1.32 (Phospholipases A1); EC 3.1.1.32 (outer membrane phospholipase A)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE


  3 / 398 MEDLINE  
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[PMID]:28283404
[Au] Autor:Rangl M; Rima L; Klement J; Miyagi A; Keller S; Scheuring S
[Ad] Endereço:Department of Anesthesiology, Physiology and Biophysics, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA; INSERM U1006, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 Avenue de Luminy, 13009 Marseille, France.
[Ti] Título:Real-time Visualization of Phospholipid Degradation by Outer Membrane Phospholipase A using High-Speed Atomic Force Microscopy.
[So] Source:J Mol Biol;429(7):977-986, 2017 Apr 07.
[Is] ISSN:1089-8638
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phospholipases are abundant in various types of cells and compartments, where they play key roles in physiological processes as diverse as digestion, cell proliferation, and neural activation. In Gram-negative bacteria, outer membrane phospholipase A (OmpLA) is involved in outer-membrane lipid homeostasis and bacterial virulence. Although the enzymatic activity of OmpLA can be probed with an assay relying on an artificial monoacyl thioester substrate, only little is known about its activity on diacyl phospholipids. Here, we used high-speed atomic force microscopy (HS-AFM) to directly image enzymatic phospholipid degradation by OmpLA in real time. In the absence of Ca , reconstituted OmpLA diffused within a phospholipid bilayer without revealing any signs of phospholipase activity. Upon the addition of Ca , OmpLA was activated and degraded the membrane with a turnover of ~2 phospholipid molecules per second and per OmpLA dimer until most of the membrane phospholipids were hydrolyzed and the protein became tightly packed.
[Mh] Termos MeSH primário: Proteínas da Membrana Bacteriana Externa/metabolismo
Microscopia de Força Atômica/métodos
Fosfolipases A1/metabolismo
Fosfolipídeos/metabolismo
[Mh] Termos MeSH secundário: Cálcio/metabolismo
Cinética
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); 0 (Phospholipids); EC 3.1.1.32 (Phospholipases A1); EC 3.1.1.32 (outer membrane phospholipase A); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170312
[St] Status:MEDLINE


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[PMID]:28143894
[Au] Autor:Emoto S; Kurano M; Kano K; Matsusaki K; Yamashita H; Nishikawa M; Igarashi K; Ikeda H; Aoki J; Kitayama J; Yatomi Y
[Ad] Endereço:Department of Surgical Oncology, University of Tokyo, Tokyo, Japan.
[Ti] Título:Analysis of glycero-lysophospholipids in gastric cancerous ascites.
[So] Source:J Lipid Res;58(4):763-771, 2017 Apr.
[Is] ISSN:1539-7262
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lysophosphatidic acid (LysoPA) has been proposed to be involved in the pathogenesis of various cancers. Moreover, glycero-lysophospholipids (glycero-LysoPLs) other than LysoPA are now emerging as novel lipid mediators. Therefore, we aimed to elucidate the possible involvement of glycero-LysoPLs in the pathogenesis of gastric cancer by measuring glycero-LysoPLs, autotaxin (ATX), and phosphatidylserine-specific phospholipase A1 (PS-PLA ) in ascites obtained from patients with gastric cancer and those with cirrhosis (as a control). We observed that after adjustments according to the albumin levels, the lysophosphatidylserine (LysoPS) and lysophosphatidylglycerol (LysoPG) levels were significantly higher, while the LysoPA and ATX levels were lower, in the ascites from patients with gastric cancer. We also found that multiple regression analyses revealed that ATX was selected as a significant explanatory factor for all the detectable LysoPA species only in the cirrhosis group and that a significant positive correlation was observed between LysoPS and PS-PLA only in the gastric cancer group. In conclusion, the LysoPA levels might be determined largely by LysoPC and LysoPI (possible precursors) and the PS-PLA -mediated pathway might be involved in the production of LysoPS in gastric cancer. Glycero-LysoPLs other than LysoPA might also be involved in the pathogenesis of cancer directly or through being converted into LysoPA.
[Mh] Termos MeSH primário: Lisofosfolipídeos/metabolismo
Fosfolipases A1/metabolismo
Neoplasias Gástricas/metabolismo
[Mh] Termos MeSH secundário: Animais
Ascite/metabolismo
Ascite/patologia
Feminino
Fibrose/metabolismo
Fibrose/patologia
Seres Humanos
Lisofosfolipídeos/isolamento & purificação
Masculino
Camundongos
Fosfolipases A1/genética
Diester Fosfórico Hidrolases
Neoplasias Gástricas/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lysophospholipids); 0 (lysophosphatidylglycerol); 0 (lysophosphatidylserine); EC 3.1.1.32 (PLA1A protein, human); EC 3.1.1.32 (Phospholipases A1); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 3.1.4.39 (alkylglycerophosphoethanolamine phosphodiesterase); PG6M3969SG (lysophosphatidic acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170202
[St] Status:MEDLINE
[do] DOI:10.1194/jlr.P072090


  5 / 398 MEDLINE  
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[PMID]:28064428
[Au] Autor:Chen W; Guo W; Gao F; Chen L; Chen S; Li D
[Ad] Endereço:Tianjin Key Laboratory for Industrial Biological Systems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesTianjin, No. 32, Xiqi Road, Tianjin Airport Economic Park, Tianjin, 300308, People's Republic of China.
[Ti] Título:Phospholipase A1-Catalysed Synthesis of Docosahexaenoic Acid-Enriched Phosphatidylcholine in Reverse Micelles System.
[So] Source:Appl Biochem Biotechnol;182(3):1037-1052, 2017 Jul.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phosphatidylcholine enriched with docosahexaenoic acid (DHA) was successfully produced by phospholipase A1-catalysed acidolysis. Reverse micelles were firstly selected as the reaction system to avoid the process of immobilization and to ensure the efficient catalysis of phospholipase A1. Parameters were optimized for a high incorporation of phosphatidylcholine enriched with DHA (DHA-PC). A response-surface design with four factors, including the water content, enzyme loading, pH and substrate-mass ratio were used to evaluate the influence of the major factors and to predict the optimal reaction conditions. The results indicated that the optimal reaction conditions for the production of DHA-PC were a water content of 0.4%, an enzyme loading of 40%, pH 6.92 and a substrate-mass ratio of 2.13. Under these optimized conditions, 20.90% of DHA content in DHA-PC was obtained.
[Mh] Termos MeSH primário: Ácidos Docosa-Hexaenoicos/síntese química
Micelas
Fosfatidilcolinas/química
Fosfolipases A1/química
[Mh] Termos MeSH secundário: Catálise
Ácidos Docosa-Hexaenoicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Micelles); 0 (Phosphatidylcholines); 25167-62-8 (Docosahexaenoic Acids); EC 3.1.1.32 (Phospholipases A1)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170109
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-016-2379-y


  6 / 398 MEDLINE  
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[PMID]:28007846
[Au] Autor:Kurano M; Kano K; Dohi T; Matsumoto H; Igarashi K; Nishikawa M; Ohkawa R; Ikeda H; Miyauchi K; Daida H; Aoki J; Yatomi Y
[Ad] Endereço:Department of Clinical Laboratory Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
[Ti] Título:Different origins of lysophospholipid mediators between coronary and peripheral arteries in acute coronary syndrome.
[So] Source:J Lipid Res;58(2):433-442, 2017 Feb.
[Is] ISSN:1539-7262
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lysophosphatidic acids (LysoPAs) and lysophosphatidylserine (LysoPS) are emerging lipid mediators proposed to be involved in the pathogenesis of acute coronary syndrome (ACS). In this study, we attempted to elucidate how LysoPA and LysoPS become elevated in ACS using human blood samples collected simultaneously from culprit coronary arteries and peripheral arteries in ACS subjects. We found that: 1) the plasma LysoPA, LysoPS, and lysophosphatidylglycerol levels were not different, while the lysophosphatidylcholine (LysoPC), lysophosphatidylinositol, and lysophosphatidylethanolamine (LysoPE) levels were significantly lower in the culprit coronary arteries; 2) the serum autotaxin (ATX) level was lower and the serum phosphatidylserine-specific phospholipase A (PS-PLA ) level was higher in the culprit coronary arteries; 3) the LysoPE and ATX levels were significant explanatory factors for the mainly elevated species of LysoPA, except for 22:6 LysoPA, in the peripheral arteries, while the LysoPC and LysoPE levels, but not the ATX level, were explanatory factors in the culprit coronary arteries; and 4) 18:0 and 18:1 LysoPS were significantly correlated with PS-PLA only in the culprit coronary arteries. In conclusion, the origins of LysoPA and LysoPS might differ between culprit coronary arteries and peripheral arteries, and substrates for ATX, such as LysoPC and LysoPE, might be important for the generation of LysoPA in ACS.
[Mh] Termos MeSH primário: Síndrome Coronariana Aguda/sangue
Aterosclerose/sangue
Vasos Coronários/metabolismo
Lisofosfolipídeos/sangue
[Mh] Termos MeSH secundário: Síndrome Coronariana Aguda/patologia
Aterosclerose/metabolismo
Aterosclerose/patologia
Vasos Coronários/patologia
Feminino
Coração/fisiopatologia
Seres Humanos
Masculino
Espectrometria de Massas
Fosfolipases A1/sangue
Diester Fosfórico Hidrolases/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lysophospholipids); 0 (lysophosphatidylglycerol); 0 (lysophosphatidylinositol); 0 (lysophosphatidylserine); EC 3.1.1.32 (Phospholipases A1); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 3.1.4.39 (alkylglycerophosphoethanolamine phosphodiesterase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161224
[St] Status:MEDLINE
[do] DOI:10.1194/jlr.P071803


  7 / 398 MEDLINE  
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[PMID]:27821336
[Au] Autor:Xin R; Khan FI; Zhao Z; Zhang Z; Yang B; Wang Y
[Ad] Endereço:College of Food Sciences and Engineering, South China University of Technology, Guangzhou 510640, PR China.
[Ti] Título:A comparative study on kinetics and substrate specificities of Phospholipase A with Thermomyces lanuginosus lipase.
[So] Source:J Colloid Interface Sci;488:149-154, 2017 Feb 15.
[Is] ISSN:1095-7103
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mechanism of lipase binding to the lipid-water interface is crucial for substrate specificity and kinetic properties. In this study, the chain-length specificity, regiospecificity and substrate specificity of Phospholipase A (PLA ) and its parent enzyme Thermomyces lanuginosus lipase (TLL) have been investigated using a classical emulsion system. The results show that both PLA and TLL are 1,3-regioselective lipases. Additionally, the hydrolytic activity of PLA is comparatively lower on short-chain triacylglyceride (TAG) and higher on phosphatidylcholine (PC) than the hydrolytic activity of TLL. Further, the results obtained with monolayer film techniques demonstrate that the C-terminal region regulates the binding of PLA to PC. A hypothesis is presented according to which the α9 helix of C-terminal region in PLA not only controls the opening of lid but also serves as a membrane anchor that assists in binding to PC. These findings bring new insight into rational design of novel lipases with intriguing functionalities.
[Mh] Termos MeSH primário: Proteínas Fúngicas/química
Lipase/química
Fosfatidilcolinas/química
Fosfolipases A1/química
Triglicerídeos/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Emulsões
Ensaios Enzimáticos
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Expressão Gênica
Cinética
Lipase/genética
Lipase/metabolismo
Fosfatidilcolinas/metabolismo
Fosfolipases A1/genética
Fosfolipases A1/metabolismo
Conformação Proteica em alfa-Hélice
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Saccharomycetales/química
Saccharomycetales/enzimologia
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Estereoisomerismo
Especificidade por Substrato
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsions); 0 (Fungal Proteins); 0 (Phosphatidylcholines); 0 (Recombinant Proteins); 0 (Triglycerides); EC 3.1.1.3 (Lipase); EC 3.1.1.32 (Phospholipases A1)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161109
[St] Status:MEDLINE


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[PMID]:27719893
[Au] Autor:Xie M; Dunford NT
[Ad] Endereço:School of Food Equipment Engineering and Science, Xi'an Jiaotong University, Xi'an 710049, China; Robert M. Kerr Food and Agricultural Products Center, Oklahoma State University, Stillwater, OK 74078, USA.
[Ti] Título:Lipid composition and emulsifying properties of canola lecithin from enzymatic degumming.
[So] Source:Food Chem;218:159-164, 2017 Mar 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study investigated the polar lipid composition and emulsifying properties of canola lecithin from enzymatic degumming (CLED). Phospholipase A was used for enzymatic degumming of crude canola oil to collect lecithin sample. Canola lecithin from water degumming (CLWD) was also collected and served as the control. The results showed that the contents of phosphatidylethanolamine (PE) (2.99%) and phosphatidylcholine (PC) (6.59%) in CLED were significantly lower than that in CLWD (PE 15.55% and PC 21.93%); while the content of lysophosphatidylcholine (LPC) (19.45%) in CLED was significantly higher than that in CLWD (3.27%). Unsaturated fatty acids accounted for a higher percentage of the total fatty acids in CLED than in CLWD. CLED promoted more stable o/w emulsions than CLWD. This study provides a better understanding of the chemical nature of CLED, and important information for utilization of CLED as o/w emulsifier.
[Mh] Termos MeSH primário: Emulsificantes/química
Ácidos Graxos Monoinsaturados/química
Ácidos Graxos/química
Lecitinas/química
Lipídeos/química
Fosfolipases A1/metabolismo
[Mh] Termos MeSH secundário: Óleo de Canola
Emulsões
Fosfatidilcolinas/química
Fosfatidiletanolaminas/química
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Canola Oil); 0 (Emulsifying Agents); 0 (Emulsions); 0 (Fatty Acids); 0 (Fatty Acids, Monounsaturated); 0 (Lecithins); 0 (Lipids); 0 (Phosphatidylcholines); 0 (Phosphatidylethanolamines); 059QF0KO0R (Water); 39382-08-6 (phosphatidylethanolamine); EC 3.1.1.32 (Phospholipases A1)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161011
[St] Status:MEDLINE


  9 / 398 MEDLINE  
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Zollner, Ricardo de Lima
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[PMID]:27826019
[Au] Autor:Perez-Riverol A; Campos Pereira FD; Musacchio Lasa A; Romani Fernandes LG; Santos-Pinto JR; Justo-Jacomini DL; Oliveira de Azevedo G; Bazon ML; Palma MS; Zollner RL; Brochetto-Braga MR
[Ad] Endereço:Laboratório de Biologia Molecular de Artrópodes-LBMA-IBRC-UNESP (Univ Estadual Paulista), Av. 24-A, nº 1515, CEP 13506-900, Bela Vista, Rio Claro, SP, Brazil. Electronic address: aperezriverol@gmail.com.
[Ti] Título:Molecular cloning, expression and IgE-immunoreactivity of phospholipase A1, a major allergen from Polybia paulista (Hymenoptera: Vespidae) venom.
[So] Source:Toxicon;124:44-52, 2016 Dec 15.
[Is] ISSN:1879-3150
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Polybia paulista (Hymenoptera: Vespidae) is a clinically relevant social wasp that frequently causes stinging accidents in southeast Brazil. To date, diagnosis and specific immunotherapy (SIT) of allergy are based on the use of crude venom extracts. Production of recombinant forms of major allergens from P. paulista venom will improve diagnosis and SIT of allergic patients by reducing the incidence of cross-reactivity and non-specific sensitization. Here, we describe the molecular cloning, heterologous expression, purification and IgE-mediated immunodetection of phospholipase A1 (Poly p 1), a major allergen from P. paulista venom. The cDNA of Poly p 1 was extracted from venom glands and then cloned, and further expression of the recombinant allergen (rPoly p 1) was achieved in Escherichia coli BL21 (DE3) cells. Purification of rPoly p 1 was performed using immobilized Ni metal affinity chromatography. Also, a single-step chromatographic method allowed the purification of native Poly p 1 (nPoly p 1) from the wasp's venom glands. We used western blotting to evaluate IgE-reactivity of the sera from 10 P. paulista venom-allergic patients to rPoly p 1 and nPoly p 1. High levels of insoluble rPoly p 1 were obtained during heterologous expression. After solubilization of inclusion bodies and purification of the recombinant protein, a unique band of ∼34 kDa was detected in SDS-PAGE analysis. Allergen-specific IgE (sIgE) from allergic patients' sera recognized rPoly p 1, nPoly p 1 and crude venom extract to a similar extent. Our results showed that rPoly p 1 could be used for development of component-resolved diagnosis (CRD) and molecular-defined SIT of P. paulista venom allergy.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Imunoglobulina E/imunologia
Fosfolipases A1/imunologia
Venenos de Vespas/imunologia
[Mh] Termos MeSH secundário: Alérgenos/química
Alérgenos/genética
Alérgenos/isolamento & purificação
Sequência de Aminoácidos
Animais
Sequência de Bases
Clonagem Molecular
DNA Complementar
Eletroforese em Gel de Poliacrilamida
Seres Humanos
Fosfolipases A1/química
Fosfolipases A1/genética
Fosfolipases A1/isolamento & purificação
Homologia de Sequência de Aminoácidos
Vespas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (DNA, Complementary); 0 (Wasp Venoms); 37341-29-0 (Immunoglobulin E); EC 3.1.1.32 (Phospholipases A1)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE


  10 / 398 MEDLINE  
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[PMID]:27746179
[Au] Autor:Urafuji K; Arioka M
[Ad] Endereço:Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
[Ti] Título:Yor022c protein is a phospholipase A that localizes to the mitochondrial matrix.
[So] Source:Biochem Biophys Res Commun;480(3):302-308, 2016 Nov 18.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In mammals, three types of intracellular phospholipase A (iPLA ) enzymes have been characterized and are thought to be involved in various cellular processes such as phospholipid metabolism, organelle biogenesis, and membrane trafficking. In this study we analyzed the unique iPLA -like protein, Yor022c, in the budding yeast Saccharomyces cerevisiae. By the mass spectrometry analysis, we demonstrate that Yor022c is actually a phospholipase displaying sn-1-specific activity toward phosphatidylcholine, phosphatidylethanolamine, and phosphatidic acid, generating 2-acyl lysophospholipids. GFP-fused Yor022c co-stained with the mitochondrial dye MitoTracker, indicating that, unlike its mammalian counterparts, it is a mitochondrial protein. Further biochemical fractionation experiment combined with protease sensitivity assay showed that Yor022c localizes to the mitochondrial matrix. Thus Yor022c is the first PLA putatively involved in the maintenance of sn-1 acyl chains of phospholipids in the mitochondrial inner membrane.
[Mh] Termos MeSH primário: Fosfolipases A1/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Ativação Enzimática
Mitocôndrias
Saccharomyces cerevisiae/ultraestrutura
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Saccharomyces cerevisiae Proteins); EC 3.1.1.32 (Phospholipases A1); EC 3.1.1.32 (Yor022c protein, S cerevisiae)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170529
[Lr] Data última revisão:
170529
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE



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