Base de dados : MEDLINE
Pesquisa : D08.811.277.352.335 [Categoria DeCS]
Referências encontradas : 9931 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 994 ir para página                         

  1 / 9931 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29176792
[Au] Autor:Tai PWL; Wu H; van Wijnen AJ; Stein GS; Stein JL; Lian JB
[Ad] Endereço:Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont, United States of America.
[Ti] Título:Genome-wide DNase hypersensitivity, and occupancy of RUNX2 and CTCF reveal a highly dynamic gene regulome during MC3T3 pre-osteoblast differentiation.
[So] Source:PLoS One;12(11):e0188056, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ability to discover regulatory sequences that control bone-related genes during development has been greatly improved by massively parallel sequencing methodologies. To expand our understanding of cis-regulatory regions critical to the control of gene expression during osteoblastogenesis, we probed the presence of open chromatin states across the osteoblast genome using global DNase hypersensitivity (DHS) mapping. Our profiling of MC3T3 mouse pre-osteoblasts during differentiation has identified more than 224,000 unique DHS sites. Approximately 65% of these sites are dynamic during temporal stages of osteoblastogenesis, and a majority of them are located within non-promoter (intergenic and intronic) regions. Nearly half of all DHS sites (both constitutive and dynamic) overlap binding events of the bone-essential RUNX2 and/or the chromatin-related CTCF transcription factors. This finding reinforces the role of these regulatory proteins as essential components of the bone gene regulome. We observe a reduction in chromatin accessibility throughout the genome between pre-osteoblast and early osteoblasts. Our analysis also defined a class of differentially expressed genes that harbor DHS peaks centered within 1 kb downstream of transcriptional end sites (TES). These DHSs at the 3'-flanks of genes exhibit dynamic changes during differentiation that may impact regulation of the osteoblast genome. Taken together, the distribution of DHS regions within non-promoter locations harboring osteoblast and chromatin related transcription factor binding motifs, reflect novel cis-regulatory requirements to support temporal gene expression in differentiating osteoblasts.
[Mh] Termos MeSH primário: Fator de Ligação a CCCTC/metabolismo
Diferenciação Celular/genética
Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo
Desoxirribonucleases/metabolismo
Regulação da Expressão Gênica
Genoma
Osteoblastos/citologia
Osteoblastos/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
DNA Intergênico/genética
Perfilação da Expressão Gênica
Ontologia Genética
Íntrons/genética
Camundongos
Ativação Transcricional/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CCCTC-Binding Factor); 0 (Core Binding Factor Alpha 1 Subunit); 0 (DNA, Intergenic); EC 3.1.- (Deoxyribonucleases)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188056


  2 / 9931 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29266956
[Au] Autor:Kougentakis CM; Grasso EM; Robinson AC; Caro JA; Schlessman JL; Majumdar A; García-Moreno E B
[Ti] Título:Anomalous Properties of Lys Residues Buried in the Hydrophobic Interior of a Protein Revealed with N-Detect NMR Spectroscopy.
[So] Source:J Phys Chem Lett;9(2):383-387, 2018 Jan 18.
[Is] ISSN:1948-7185
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ionizable residues buried in hydrophobic environments in proteins are essential for many fundamental biochemical processes. These residues titrate with anomalous pK values that are challenging to reproduce with structure-based calculations owing to the conformational reorganization coupled to their ionization. Detailed characterization of this conformational reorganization is of interest; unfortunately, the properties of buried Lys residues are difficult to study experimentally. Here we demonstrate the utility of N NMR spectroscopy to gain insight into the protonation state, state of hydration and conformational dynamics of the Nζ amino group of buried Lys residues. The experiments were applied to five variants of staphylococcal nuclease, with internal Lys residues that titrate with pK values ranging from 6.2 to 8.1. Direct detection of buried Lys residues with these NMR spectroscopy methods will enable correlation between thermodynamic and structural data as well as unprecedented examination of how conformational transitions coupled to their ionization affect their pK values.
[Mh] Termos MeSH primário: Desoxirribonucleases/química
Espectroscopia de Ressonância Magnética
Nuclease do Micrococo/química
[Mh] Termos MeSH secundário: Interações Hidrofóbicas e Hidrofílicas
Conformação Proteica
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.1.- (Deoxyribonucleases); EC 3.1.31.1 (Micrococcal Nuclease)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jpclett.7b02668


  3 / 9931 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29284001
[Au] Autor:Kang D; Sherwood R; Barkal A; Hashimoto T; Engstrom L; Gifford D
[Ad] Endereço:Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
[Ti] Título:DNase-capture reveals differential transcription factor binding modalities.
[So] Source:PLoS One;12(12):e0187046, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We describe DNase-capture, an assay that increases the analytical resolution of DNase-seq by focusing its sequencing phase on selected genomic regions. We introduce a new method to compensate for capture bias called BaseNormal that allows for accurate recovery of transcription factor protection profiles from DNase-capture data. We show that these normalized data allow for nuanced detection of transcription factor binding heterogeneity with as few as dozens of sites.
[Mh] Termos MeSH primário: Desoxirribonucleases/metabolismo
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Sítios de Ligação
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Transcription Factors); EC 3.1.- (Deoxyribonucleases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187046


  4 / 9931 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29191890
[Au] Autor:Gunzer M
[Ad] Endereço:University of Duisburg-Essen, University Hospital, Institute for Experimental Immunology and Imaging, Essen, Germany. matthias.gunzer@uni-due.de.
[Ti] Título:Escaping the traps of your own hunters.
[So] Source:Science;358(6367):1126-1127, 2017 12 01.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Armadilhas Extracelulares
Neutrófilos
[Mh] Termos MeSH secundário: DNA/metabolismo
Desoxirribonucleases
Infecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Nm] Nome de substância:
9007-49-2 (DNA); EC 3.1.- (Deoxyribonucleases)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171206
[Lr] Data última revisão:
171206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1126/science.aar2428


  5 / 9931 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29020107
[Au] Autor:Temprana CF; Prieto MJ; Igartúa DE; Femia AL; Amor MS; Alonso SDV
[Ad] Endereço:Laboratorio de Biomembranas (LBM), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Argentina.
[Ti] Título:Diacetylenic lipids in the design of stable lipopolymers able to complex and protect plasmid DNA.
[So] Source:PLoS One;12(10):e0186194, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Different viral and non-viral vectors have been designed to allow the delivery of nucleic acids in gene therapy. In general, non-viral vectors have been associated with increased safety for in vivo use; however, issues regarding their efficacy, toxicity and stability continue to drive further research. Thus, the aim of this study was to evaluate the potential use of the polymerizable diacetylenic lipid 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC8,9PC) as a strategy to formulate stable cationic lipopolymers in the delivery and protection of plasmid DNA. Cationic lipopolymers were prepared following two different methodologies by using DC8,9PC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and the cationic lipids (CL) 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), stearylamine (SA), and myristoylcholine chloride (MCL), in a molar ratio of 1:1:0.2 (DMPC:DC8,9PC:CL). The copolymerization methodology allowed obtaining cationic lipopolymers which were smaller in size than those obtained by the cationic addition methodology although both techniques presented high size stability over a 166-day incubation period at 4°C. Cationic lipopolymers containing DOTAP or MCL were more efficient in complexing DNA than those containing SA. Moreover, lipopolymers containing DOTAP were found to form highly stable complexes with DNA, able to resist serum DNAses degradation. Furthermore, neither of the cationic lipopolymers (with or without DNA) induced red blood cell hemolysis, although metabolic activity determined on the L-929 and Vero cell lines was found to be dependent on the cell line, the formulation and the presence of DNA. The high stability and DNA protection capacity as well as the reduced toxicity determined for the cationic lipopolymer containing DOTAP highlight the potential advantage of using lipopolymers when designing novel non-viral carrier systems for use in in vivo gene therapy. Thus, this work represents the first steps toward developing a cationic lipopolymer-based gene delivery system using polymerizable and cationic lipids.
[Mh] Termos MeSH primário: Acetileno/química
DNA/metabolismo
Lipídeos/química
Plasmídeos/metabolismo
Polímeros/síntese química
[Mh] Termos MeSH secundário: Animais
Bioensaio
Células COS
Cátions
Sobrevivência Celular
Cercopithecus aethiops
Desoxirribonucleases/metabolismo
Ensaio de Desvio de Mobilidade Eletroforética
Citometria de Fluxo
Hemólise
Luz
Camundongos
Peso Molecular
Polimerização
Polímeros/química
Espalhamento de Radiação
Eletricidade Estática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations); 0 (Lipids); 0 (Polymers); 9007-49-2 (DNA); EC 3.1.- (Deoxyribonucleases); OC7TV75O83 (Acetylene)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186194


  6 / 9931 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28873986
[Au] Autor:Niyonzima N; Lambert AR; Werther R; De Silva Feelixge H; Roychoudhury P; Greninger AL; Stone D; Stoddard BL; Jerome KR
[Ad] Endereço:Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA 98109, USA.
[Ti] Título:Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses.
[So] Source:Protein Eng Des Sel;30(7):503-522, 2017 Jul 01.
[Is] ISSN:1741-0134
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The combination of yeast surface display and flow cytometric analyses and selections is being used with increasing frequency to alter specificity of macromolecular recognition, including both protein-protein and protein-nucleic acid interactions. Here we describe the use of yeast surface display and cleavage-dependent flow cytometric assays to increase the specificity of an engineered meganuclease. The re-engineered meganuclease displays a significantly tightened specificity profile, while binding its cognate target site with a slightly lower, but still sub-nanomolar affinity. When incorporated into otherwise identical megaTAL protein scaffolds, these two nucleases display significantly different activity and toxicity profiles in cellulo. The structural basis for reprogrammed DNA cleavage specificity was further examined via high-resolution X-ray crystal structures of both enzymes. This analysis illustrated the altered protein-DNA contacts produced by mutagenesis and selection, that resulted both in altered readout of those based and a necessary reduction in DNA binding affinity that were necessary to improve specificity across the target site. The results of this study provide an illustrative example of the potential (and the challenges) associated with the use of surface display and flow cytometry for the retargeting and optimization of enzymes that act on nucleic acid substrates in a sequence-specific manner.
[Mh] Termos MeSH primário: DNA/genética
Desoxirribonucleases/química
Endonucleases/genética
Engenharia Metabólica
[Mh] Termos MeSH secundário: Sítios de Ligação
DNA/química
Clivagem do DNA
Desoxirribonucleases/genética
Endonucleases/química
Citometria de Fluxo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA); EC 3.1.- (Deoxyribonucleases); EC 3.1.- (Endonucleases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1093/protein/gzx037


  7 / 9931 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28780455
[Au] Autor:Nath M; Mridula; Kumari R
[Ad] Endereço:Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India.. Electronic address: malanfcy@iitr.ernet.in.
[Ti] Título:Microwave-assisted synthesis of mixed ligands organotin(IV) complexes of 1,10-phenanthroline and l-proline: Physicochemical characterization, DFT calculations, chemotherapeutic potential validation by in vitro DNA binding and nuclease activity.
[So] Source:J Photochem Photobiol B;174:182-194, 2017 Sep.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Diorganotin(IV) and triphenyltin(IV) derivatives of L-proline (HPro) having general formula R Sn(Pro) (R=n-Bu (1), Ph (2)) and Ph Sn(Pro) (3), respectively, and the mixed ligands di-/triorganotin(IV) derivatives of L-proline and 1,10-phenanthroline (phen) with general formula [R Sn(Pro)(Phen)Cl] and [R Sn(Pro)(Phen)] (where R=Me (4 and 7), n-Bu (5 and 8), Ph (6 and 9)), respectively, have been synthesized by microwave-assisted method and characterized by elemental analysis, IR, NMR ( H, C and Sn) and DART-mass spectral studies. The results suggest bicapped tetrahedron or a skew trapezoidal-bipyramid geometry for R Sn(Pro) , a distorted tetrahedral geometry for Ph Sn(Pro) and a distorted octahedral geometry for [R Sn(Pro)(Phen)Cl] and [Ph Sn(Pro)(Phen)] around the Sn atom, and the same has been validated by density functional theory calculations (DFT). In vitro DNA binding studies of 1-9 have been investigated by UV-Vis, fluorescence and circular dichroism titrations, viscosity and DNA melting experiments. The observed hypochromic shift in UV-Vis and fluorescence studies evidenced a partial intercalative mode of binding of complexes to CT-DNA. The binding affinity and quenching ability have been quantified in terms of intrinsic binding constant (K ) and Stern-Volmer quenching constant (Ksv). The determined values suggest that di- and triorganotin(IV) derivatives of L-proline possess lesser affinity to bind with CT-DNA in comparison to the mixed ligands di-/triorganotin(IV) derivatives of L-proline and 1,10-phenanthroline. The partial intercalative mode of binding of these complexes with CT DNA has also been supported by a change in the viscosity and melting point of DNA as well as a change in the intensity of positive and negative bands in circular dichroism spectra. The cleavage studies by agarose gel electrophoresis indicate effective cleavage of supercoiled plasmid DNA into its nicked form by all the complexes and even to its linear form in presence of 9.
[Mh] Termos MeSH primário: DNA/metabolismo
Desoxirribonucleases/metabolismo
Micro-Ondas
Compostos Orgânicos de Estanho/química
Compostos Orgânicos de Estanho/metabolismo
Fenantrolinas/química
Prolina/química
[Mh] Termos MeSH secundário: Fenômenos Químicos
Técnicas de Química Sintética
DNA/química
Ligantes
Modelos Moleculares
Conformação Molecular
Desnaturação de Ácido Nucleico
Compostos Orgânicos de Estanho/síntese química
Compostos Orgânicos de Estanho/farmacologia
Teoria Quântica
Temperatura de Transição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ligands); 0 (Organotin Compounds); 0 (Phenanthrolines); 9007-49-2 (DNA); 9DLQ4CIU6V (Proline); EC 3.1.- (Deoxyribonucleases); W4X6ZO7939 (1,10-phenanthroline)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170807
[St] Status:MEDLINE


  8 / 9931 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28715501
[Au] Autor:Sharp C; Bray J; Housden NG; Maiden MCJ; Kleanthous C
[Ad] Endereço:Department of Biochemistry, University of Oxford, Oxford, United Kingdom.
[Ti] Título:Diversity and distribution of nuclease bacteriocins in bacterial genomes revealed using Hidden Markov Models.
[So] Source:PLoS Comput Biol;13(7):e1005652, 2017 Jul.
[Is] ISSN:1553-7358
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteria exploit an arsenal of antimicrobial peptides and proteins to compete with each other. Three main competition systems have been described: type six secretion systems (T6SS); contact dependent inhibition (CDI); and bacteriocins. Unlike T6SS and CDI systems, bacteriocins do not require contact between bacteria but are diffusible toxins released into the environment. Identified almost a century ago, our understanding of bacteriocin distribution and prevalence in bacterial populations remains poor. In the case of protein bacteriocins, this is because of high levels of sequence diversity and difficulties in distinguishing their killing domains from those of other competition systems. Here, we develop a robust bioinformatics pipeline exploiting Hidden Markov Models for the identification of nuclease bacteriocins (NBs) in bacteria of which, to-date, only a handful are known. NBs are large (>60 kDa) toxins that target nucleic acids (DNA, tRNA or rRNA) in the cytoplasm of susceptible bacteria, usually closely related to the producing organism. We identified >3000 NB genes located on plasmids or on the chromosome from 53 bacterial species distributed across different ecological niches, including human, animals, plants, and the environment. A newly identified NB predicted to be specific for Pseudomonas aeruginosa (pyocin Sn) was produced and shown to kill P. aeruginosa thereby validating our pipeline. Intriguingly, while the genes encoding the machinery needed for NB translocation across the cell envelope are widespread in Gram-negative bacteria, NBs are found exclusively in γ-proteobacteria. Similarity network analysis demonstrated that NBs fall into eight groups each with a distinct arrangement of protein domains involved in import. The only structural feature conserved across all groups was a sequence motif critical for cell-killing that is generally not found in bacteriocins targeting the periplasm, implying a specific role in translocating the nuclease to the cytoplasm. Finally, we demonstrate a significant association between nuclease colicins, NBs specific for Escherichia coli, and virulence factors, suggesting NBs play a role in infection processes, most likely by enabling pathogens to outcompete commensal bacteria.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Bacteriocinas/genética
Desoxirribonucleases/genética
Gammaproteobacteria/genética
Genoma Bacteriano/genética
[Mh] Termos MeSH secundário: Anti-Infecciosos
Proteínas de Bactérias/metabolismo
Simulação por Computador
Desoxirribonucleases/metabolismo
Gammaproteobacteria/enzimologia
Genoma Bacteriano/fisiologia
Cadeias de Markov
Pseudomonas aeruginosa/enzimologia
Pseudomonas aeruginosa/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Bacterial Proteins); 0 (Bacteriocins); EC 3.1.- (Deoxyribonucleases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pcbi.1005652


  9 / 9931 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28714971
[Au] Autor:Lengefeld J; Hotz M; Rollins M; Baetz K; Barral Y
[Ad] Endereço:Institute of Biochemistry, ETH Zurich, Otto-Stern-Weg 3, 8093 Zurich, Switzerland.
[Ti] Título:Budding yeast Wee1 distinguishes spindle pole bodies to guide their pattern of age-dependent segregation.
[So] Source:Nat Cell Biol;19(8):941-951, 2017 Aug.
[Is] ISSN:1476-4679
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many asymmetrically dividing cells unequally partition cellular structures according to age. Yet, it is unclear how cells differentiate pre-existing from newly synthesized material. Yeast cells segregate the spindle pole body (SPB, centrosome equivalent) inherited from the previous mitosis to the bud, while keeping the new one in the mother cell. Here, we show that the SPB inheritance network (SPIN), comprising the kinases Swe1 (also known as Wee1) and Kin3 (also known as Nek2) and the acetyltransferase NuA4 (also known as Tip60), distinguishes pre-existing from new SPBs. Swe1 phosphorylated Nud1 (orthologous to Centriolin) on young SPBs as they turned into pre-existing ones. The subsequent inactivation of Swe1 protected newly assembling SPBs from being marked. Kin3 and NuA4 maintained age marks on SPBs through following divisions. Downstream of SPIN, the Hippo regulator Bfa1-Bub2 bound the marked SPB, directed the spindle-positioning protein Kar9 towards it and drove its partition to the bud. Thus, coordination of SPIN activity and SPB assembly encodes age onto SPBs to enable their age-dependent segregation.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/metabolismo
Segregação de Cromossomos
Cromossomos Fúngicos
Proteínas Tirosina Quinases/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/metabolismo
Fuso Acromático/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Ciclo Celular/genética
Proliferação Celular
Proteínas do Citoesqueleto/genética
Proteínas do Citoesqueleto/metabolismo
Desoxirribonucleases/genética
Desoxirribonucleases/metabolismo
Fase G1
Regulação Fúngica da Expressão Gênica
Histona Acetiltransferases/genética
Histona Acetiltransferases/metabolismo
Metáfase
Proteínas Nucleares/genética
Proteínas Nucleares/metabolismo
Fosforilação
Proteínas Serina-Treonina Quinases/genética
Proteínas Serina-Treonina Quinases/metabolismo
Proteínas Tirosina Quinases/genética
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/crescimento & desenvolvimento
Proteínas de Saccharomyces cerevisiae/genética
Transdução de Sinais
Fatores de Tempo
tRNA Metiltransferases/genética
tRNA Metiltransferases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BFA1 protein, S cerevisiae); 0 (BUB2 protein, S cerevisiae); 0 (Cell Cycle Proteins); 0 (Cytoskeletal Proteins); 0 (KAR9 protein, S cerevisiae); 0 (Nuclear Proteins); 0 (Saccharomyces cerevisiae Proteins); EC 2.1.1.- (tRNA Methyltransferases); EC 2.3.1.48 (Histone Acetyltransferases); EC 2.3.1.48 (NuA4 protein, S cerevisiae); EC 2.7.1.- (SWE1 protein, S cerevisiae); EC 2.7.10.1 (Protein-Tyrosine Kinases); EC 2.7.11.1 (KIN3 protein, S cerevisiae); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 3.1.- (Deoxyribonucleases); EC 3.1.- (TRM2 protein, S cerevisiae)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1038/ncb3576


  10 / 9931 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28666955
[Au] Autor:Keyel PA
[Ad] Endereço:Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, United States. Electronic address: peter.keyel@ttu.edu.
[Ti] Título:Dnases in health and disease.
[So] Source:Dev Biol;429(1):1-11, 2017 09 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA degradation is critical to healthy organism development and survival. Two nuclease families that play key roles in development and in disease are the Dnase1 and Dnase2 families. While these two families were initially characterized by biochemical function, it is now clear that multiple enzymes in each family perform similar, non-redundant roles in many different tissues. Most Dnase1 and Dnase2 family members are poorly characterized, yet their elimination can lead to a wide range of diseases, including lethal anemia, parakeratosis, cataracts and systemic lupus erythematosus. Therefore, understanding these enzyme families represents a critical field of emerging research. This review explores what is currently known about Dnase1 and Dnase2 family members, highlighting important questions about the structure and function of family members, and how their absence translates to disease.
[Mh] Termos MeSH primário: Desoxirribonucleases/metabolismo
Doença
Saúde
[Mh] Termos MeSH secundário: Animais
Desoxirribonucleases/química
Seres Humanos
Especificidade de Órgãos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.1.- (Deoxyribonucleases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170702
[St] Status:MEDLINE



página 1 de 994 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde