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[PMID]:17953481
[Au] Autor:Grandgenett PM; Otsu K; Wilson HR; Wilson ME; Donelson JE
[Ad] Endereço:Interdepartmental Genetics Program, University of Iowa, Iowa City, Iowa, USA.
[Ti] Título:A function for a specific zinc metalloprotease of African trypanosomes.
[So] Source:PLoS Pathog;3(10):1432-45, 2007 Oct 19.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Trypanosoma brucei genome encodes three groups of zinc metalloproteases, each of which contains approximately 30% amino acid identity with the major surface protease (MSP, also called GP63) of Leishmania. One of these proteases, TbMSP-B, is encoded by four nearly identical, tandem genes transcribed in both bloodstream and procyclic trypanosomes. Earlier work showed that RNA interference against TbMSP-B prevents release of a recombinant variant surface glycoprotein (VSG) from procyclic trypanosomes. Here, we used gene deletions to show that TbMSP-B and a phospholipase C (GPI-PLC) act in concert to remove native VSG during differentiation of bloodstream trypanosomes to procyclic form. When the four tandem TbMSP-B genes were deleted from both chromosomal alleles, bloodstream B (-/-) trypanosomes could still differentiate to procyclic form, but VSG was removed more slowly and in a non-truncated form compared to differentiation of wild-type organisms. Similarly, when both alleles of the single-copy GPI-PLC gene were deleted, bloodstream PLC (-/-) cells could still differentiate. However, when all the genes for both TbMSP-B and GPI-PLC were deleted from the diploid genome, the bloodstream B (-/-) PLC (-/-) trypanosomes did not proliferate in the differentiation medium, and 60% of the VSG remained on the cell surface. Inhibitors of cysteine proteases did not affect this result. These findings demonstrate that removal of 60% of the VSG during differentiation from bloodstream to procyclic form is due to the synergistic activities of GPI-PLC and TbMSP-B.
[Mh] Termos MeSH primário: Metaloproteases/metabolismo
Proteínas de Protozoários/biossíntese
Trypanosoma brucei brucei/enzimologia
Glicoproteínas Variantes de Superfície de Trypanosoma/biossíntese
[Mh] Termos MeSH secundário: Animais
Antígenos de Protozoários
Linhagem Celular
Deleção de Genes
Dosagem de Genes
Glicosilfosfatidilinositol Diacilglicerol-Liase
Estágios do Ciclo de Vida/fisiologia
Metaloendopeptidases/biossíntese
Metaloendopeptidases/genética
Metaloproteases/genética
Fosfatidilinositol Diacilglicerol-Liase/genética
Fosfatidilinositol Diacilglicerol-Liase/metabolismo
Proteínas de Protozoários/genética
Trypanosoma brucei brucei/genética
Glicoproteínas Variantes de Superfície de Trypanosoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antigens, Protozoan); 0 (Protozoan Proteins); 0 (Variant Surface Glycoproteins, Trypanosoma); EC 3.4.- (Metalloproteases); EC 3.4.24.- (Metalloendopeptidases); EC 3.4.24.- (glycoprotein gp63, Leishmania); EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase); EC 4.6.1.14 (Glycosylphosphatidylinositol Diacylglycerol-Lyase)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071024
[St] Status:MEDLINE


  2 / 1403 MEDLINE  
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[PMID]:17652793
[Au] Autor:Echevarría-Machado I; Martínez-Estévez M; Muñoz-Sánchez JA; Loyola-Vargas VM; Hernández-Sotomayor SM; De Los Santos-Briones C
[Ad] Endereço:Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigaciòn Científica de Yucatán A.C., Calle 43 No. 130, Chuburná de Hidalgo, C. P. 97200, Mérida, Yucatán, México.
[Ti] Título:Membrane-associated phosphoinositides-specific phospholipase C forms from Catharanthus roseus transformed roots.
[So] Source:Mol Biotechnol;35(3):297-309, 2007 Mar.
[Is] ISSN:1073-6085
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have previously reported that Catharanthus roseus transformed roots contain at least two phosphatidylinositol 4,5-bisphosphate-phospholipase C (PLC) activities, one soluble and the other membrane associated. Detergent, divalent cations, and neomycin differentially regulate these activities and pure protein is required for a greater understanding of the function and regulation of this enzyme. In this article we report a partia purification of membrane-associated PLC. We found that there are at least two forms of membraneassociated PLC in transformed roots of C. roseus. These forms were separated on the basis of their affinity for heparin. One form shows an affinity for heparin and elutes at approx 600 mM KCl. This form has a molecular mass of 67 kDa by size exclusion chromatography and Western blot analysis, whereas the other form does not bind to heparin and has a molecular mass of 57 kDa. Possible differential regulation of these forms during transformed root growth is discussed.
[Mh] Termos MeSH primário: Fosfatidilinositol Diacilglicerol-Liase/metabolismo
Raízes de Plantas/enzimologia
[Mh] Termos MeSH secundário: Western Blotting
Catharanthus/enzimologia
Cromatografia Líquida
Eletroforese em Gel de Poliacrilamida
Fosfatidilinositol Diacilglicerol-Liase/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase)
[Em] Mês de entrada:0709
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070727
[St] Status:MEDLINE


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[PMID]:17628524
[Au] Autor:Shimizu T; Yamaguchi N; Okada S; Lu L; Sasaki T; Yokotani K
[Ad] Endereço:Department of Pharmacology, Graduate School of Medicine, Kochi University, Nankoku, Kochi 783-8505, Japan.
[Ti] Título:Roles of brain phosphatidylinositol-specific phospholipase C and diacylglycerol lipase in centrally administered histamine-induced adrenomedullary outflow in rats.
[So] Source:Eur J Pharmacol;571(2-3):138-44, 2007 Oct 01.
[Is] ISSN:0014-2999
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Recently, we reported that intracerebroventricularly (i.c.v.) administered histamine evokes the secretion of noradrenaline and adrenaline from adrenal medulla by brain cyclooxygenase-1- and thromboxane A2-mediated mechanisms in rats. These results suggest the involvement of brain arachidonic acid cascade in the histamine-induced activation of the central adrenomedullary outflow. Arachidonic acid is released mainly by phospholipase A2 (PLA2)-dependent pathway or phospholipase C (PLC)/diacylglycerol lipase-dependent pathway. In the present study, histamine (27 nmol/animal, i.c.v.) -induced elevation of plasma noradrenaline and adrenaline was dose-dependently reduced by U-73122 (PLC inhibitor) (10 and 100 nmol/animal, i.c.v.), ET-18-OCH3 (phosphatidylinositol-specific PLC inhibitor) (10 and 30 nmol/animal, i.c.v.) and RHC-80267 (diacylglycerol lipase inhibitor) (1.3 and 2.6 micromol/animal, i.c.v.). However, mepacrine (PLA2 inhibitor) (1.1 and 2.2 micromol/animal, i.c.v.) and D609 (phosphatidylcholine-specific PLC inhibitor) (30, 100 and 300 nmol/animal, i.c.v.) had no effect. These results suggest the involvement of brain phosphatidylinositol-specific PLC and diacylglycerol lipase in the centrally administered histamine-induced activation of the adrenomedullary outflow in rats.
[Mh] Termos MeSH primário: Medula Suprarrenal/metabolismo
Encéfalo/metabolismo
Epinefrina/sangue
Histamina/metabolismo
Lipase Lipoproteica/metabolismo
Norepinefrina/sangue
Fosfatidilinositol Diacilglicerol-Liase/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Ácido Araquidônico/metabolismo
Encéfalo/efeitos dos fármacos
Encéfalo/enzimologia
Hidrocarbonetos Aromáticos com Pontes/farmacologia
Cicloexanonas/farmacologia
Relação Dose-Resposta a Droga
Inibidores Enzimáticos/farmacologia
Estrenos/farmacologia
Histamina/administração & dosagem
Injeções Intraventriculares
Lipase Lipoproteica/antagonistas & inibidores
Masculino
Fosfatidilinositol Diacilglicerol-Liase/antagonistas & inibidores
Fosfoinositídeo Fosfolipase C
Fosfolipases A/antagonistas & inibidores
Fosfolipases A/metabolismo
Fosfolipases A2
Éteres Fosfolipídicos/farmacologia
Pirrolidinonas/farmacologia
Quinacrina/farmacologia
Ratos
Ratos Wistar
Transdução de Sinais/efeitos dos fármacos
Tionas/farmacologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bridged-Ring Compounds); 0 (Cyclohexanones); 0 (Enzyme Inhibitors); 0 (Estrenes); 0 (Phospholipid Ethers); 0 (Pyrrolidinones); 0 (Thiones); 112648-68-7 (1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione); 1Y6SNA8L5S (edelfosine); 27YG812J1I (Arachidonic Acid); 820484N8I3 (Histamine); 83373-60-8 (tricyclodecane-9-yl-xanthogenate); 83654-05-1 (1,6-bis(cyclohexyloximinocarbonyl)hexane); EC 3.1.1.32 (Phospholipases A); EC 3.1.1.34 (Lipoprotein Lipase); EC 3.1.1.4 (Phospholipases A2); EC 3.1.4.11 (Phosphoinositide Phospholipase C); EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase); H0C805XYDE (Quinacrine); X4W3ENH1CV (Norepinephrine); YKH834O4BH (Epinephrine)
[Em] Mês de entrada:0710
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070714
[St] Status:MEDLINE


  4 / 1403 MEDLINE  
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[PMID]:17495162
[Au] Autor:Irvine R
[Ad] Endereço:Department of Pharmacology, University of Cambridge, Cambridge CB2 1PD, UK. rfi20@cam.ac.uk
[Ti] Título:Cell signaling. The art of the soluble.
[So] Source:Science;316(5826):845-6, 2007 May 11.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Fosfatos de Inositol/metabolismo
Proteínas Tirosina Quinases/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Inositol 1,4,5-Trifosfato/metabolismo
Linfócitos/fisiologia
Camundongos
Fosfatidilinositol Diacilglicerol-Liase/genética
Fosfatidilinositol Diacilglicerol-Liase/metabolismo
Fosfatos de Fosfatidilinositol/metabolismo
Fosforilação
Fosfotransferases (Aceptor do Grupo Fosfato)/química
Fosfotransferases (Aceptor do Grupo Fosfato)/genética
Fosfotransferases (Aceptor do Grupo Fosfato)/metabolismo
Estrutura Terciária de Proteína
Proteínas Tirosina Quinases/química
Receptores Citoplasmáticos e Nucleares/metabolismo
Proteínas Repressoras/metabolismo
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/crescimento & desenvolvimento
Saccharomyces cerevisiae/metabolismo
Proteínas de Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/metabolismo
Schizosaccharomyces/genética
Schizosaccharomyces/metabolismo
Sistemas do Segundo Mensageiro
Solubilidade
[Pt] Tipo de publicação:COMMENT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Inositol Phosphates); 0 (PHO81 protein, S cerevisiae); 0 (Phosphatidylinositol Phosphates); 0 (Receptors, Cytoplasmic and Nuclear); 0 (Repressor Proteins); 0 (Saccharomyces cerevisiae Proteins); 0 (inositol heptakisphosphate); 0 (inositol-1,3,4,5-tetrakisphosphate receptor); 0 (phosphatidylinositol 3,4,5-triphosphate); 102850-29-3 (inositol-1,3,4,5-tetrakisphosphate); 85166-31-0 (Inositol 1,4,5-Trisphosphate); EC 2.7.1.- (Tec protein-tyrosine kinase); EC 2.7.10.1 (Agammaglobulinaemia tyrosine kinase); EC 2.7.10.1 (Protein-Tyrosine Kinases); EC 2.7.10.2 (emt protein-tyrosine kinase); EC 2.7.4.- (Phosphotransferases (Phosphate Group Acceptor)); EC 2.7.4.21 (KCS1 protein, S cerevisiae); EC 2.7.4.21 (inositol hexakisphosphate kinase); EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase)
[Em] Mês de entrada:0705
[Cu] Atualização por classe:120601
[Lr] Data última revisão:
120601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070515
[St] Status:MEDLINE


  5 / 1403 MEDLINE  
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[PMID]:17488650
[Au] Autor:Liu DZ; Liang HJ; Chen CH; Lin SY; Zhong WB; Ho FM; Hou WC; Lo JL; Ho YS; Lin PJ; Hung LF; Liang YC
[Ad] Endereço:Graduate Institutes of Biomedical Materials, Taipei Medical University, Taipei, Taiwan.
[Ti] Título:Switch activation of PI-PLC downstream signals in activated macrophages with wortmannin.
[So] Source:Biochim Biophys Acta;1773(6):869-79, 2007 Jun.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Phosphatidylinositol (4,5)-bisphosphate (PtdIns(4,5)P(2)) has been known to serve as a substrate for phosphatidylinositol 3-kinase (PI(3)K) and phosphoinositide-specific phospholipase C (PI-PLC), which can produce PtdIns(3,4,5)P(3) and inositol 1,4,5-trisphosphate (Ins(1,4,5)P(3)) and diacylglycerol (DAG), respectively. In this study, we elucidated the role of PI-PLC during the LPS-activated mouse macrophages RAW264.7 treated with PI(3)K inhibitor wortmannin. First, wortmannin treatment enhanced Ins(1,4,5)P(3) production and iNOS expression in LPS-activated macrophages. Inhibition of PI(3)K by p85 siRNA also showed an enhancement of iNOS expression. On the other hand, overexpression of PI(3)K by ras-p110 expression plasmid significantly decreased iNOS expression in LPS-activated macrophages. In addition, overexpression of wild-type or dominant-negative Akt expression plasmid did not affect the iNOS expression in LPS-activated macrophages. Second, treatment of PI-PLC inhibitor U73122 reversed the enhancement of iNOS expression, the increase of phosphorylation level of ERK, JNK and p38, and the increase of AP-1-dependent gene expression in wortmannin-treated and LPS-activated macrophages. However, NF-kappaB activity determined by EMSA assay and reporter plasmid assay did not change during LPS-activated macrophages with or without wortmannin. We propose that the inhibition of PI(3)K by wortmannin in mouse macrophages enhances the PI-PLC downstream signals, and subsequently increases the LPS induction of iNOS expression independently of Akt pathway.
[Mh] Termos MeSH primário: Androstadienos/farmacologia
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Ativação de Macrófagos/efeitos dos fármacos
Macrófagos/enzimologia
Fosfatidilinositol Diacilglicerol-Liase/metabolismo
Inibidores de Fosfodiesterase/farmacologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Diglicerídeos/metabolismo
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Inositol 1,4,5-Trifosfato/metabolismo
Lipopolissacarídeos/metabolismo
Camundongos
NF-kappa B/metabolismo
Óxido Nítrico Sintase Tipo II/metabolismo
Fosfatidilinositol 3-Quinases/antagonistas & inibidores
Fosfatidilinositol 4,5-Difosfato/metabolismo
Fosfoinositídeo Fosfolipase C
Proteínas Proto-Oncogênicas c-akt/metabolismo
Fator de Transcrição AP-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Androstadienes); 0 (Diglycerides); 0 (Lipopolysaccharides); 0 (NF-kappa B); 0 (Phosphatidylinositol 4,5-Diphosphate); 0 (Phosphodiesterase Inhibitors); 0 (Transcription Factor AP-1); 85166-31-0 (Inositol 1,4,5-Trisphosphate); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nos2 protein, mouse); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 3.1.4.11 (Phosphoinositide Phospholipase C); EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase); XVA4O219QW (wortmannin)
[Em] Mês de entrada:0707
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070510
[St] Status:MEDLINE


  6 / 1403 MEDLINE  
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PubMed Central Texto completo
[PMID]:17363325
[Au] Autor:Lukinovic-Skudar V; Matkovic K; Banfic H; Visnjic D
[Ad] Endereço:Department of Physiology and Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Salata 3, Zagreb, Croatia.
[Ti] Título:Two waves of the nuclear phospholipase C activity in serum-stimulated HL-60 cells during G(1) phase of the cell cycle.
[So] Source:Biochim Biophys Acta;1771(4):514-21, 2007 Apr.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Phosphatidylinositol-specific phospholipase C (PI-PLC) is activated in cell nuclei during the cell cycle progression. We have previously demonstrated two peaks of an increase in the nuclear PI-PLC activities in nocodazole-synchronized HL-60 cells. In this study, the activity of nuclear PI-PLC was investigated in serum-stimulated HL-60 cells. In serum-starved HL-60 cells, two peaks of the activity of nuclear PI-PLC were detected at 30 min and 11 h after the re-addition of serum with no parallel increase in PLC activity in cytosol, postnuclear membranes or total cell lysates. An increase in the serine phosphorylation of b splicing variant of PI-PLCbeta(1) was detected with no change in the amount of PI-PLCbeta(1b) in nuclei isolated at 30 min and 11 h after the addition of serum. PI-PLC inhibitor ET-18-OCH(3) and MEK inhibitor PD 98059 completely abolished serum-mediated increase at both time-points. The addition of inhibitors either immediately or 6 h after the addition of serum had inhibitory effects on the number of cells entering S phase. These results demonstrate that two waves of nuclear PI-PLCbeta(1b) activity occur in serum-stimulated cells during G(1) phase of the cell cycle and that the later increase in the PLC activity is equally important for the progression into the S phase.
[Mh] Termos MeSH primário: Núcleo Celular/enzimologia
Fase G1
Fosfatidilinositol Diacilglicerol-Liase/metabolismo
Soro/metabolismo
[Mh] Termos MeSH secundário: Animais
Bovinos
Núcleo Celular/efeitos dos fármacos
Inibidores Enzimáticos/farmacologia
Fase G1/efeitos dos fármacos
Células HL-60
Seres Humanos
Fosfatidilinositol Diacilglicerol-Liase/antagonistas & inibidores
Fosfoinositídeo Fosfolipase C
Fosfosserina/metabolismo
Frações Subcelulares/efeitos dos fármacos
Frações Subcelulares/enzimologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 17885-08-4 (Phosphoserine); EC 3.1.4.11 (Phosphoinositide Phospholipase C); EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase)
[Em] Mês de entrada:0705
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070317
[St] Status:MEDLINE


  7 / 1403 MEDLINE  
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[PMID]:17213187
[Au] Autor:Shao C; Shi X; Wehbi H; Zambonelli C; Head JF; Seaton BA; Roberts MF
[Ad] Endereço:Boston College, Chestnut Hill, Massachusetts 02467, USA.
[Ti] Título:Dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase C.
[So] Source:J Biol Chem;282(12):9228-35, 2007 Mar 23.
[Is] ISSN:0021-9258
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The crystal structure of the W47A/W242A mutant of phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus thuringiensis has been solved to 1.8A resolution. The W47A/W242A mutant is an interfacially challenged enzyme, and it has been proposed that one or both tryptophan side chains serve as membrane interfacial anchors (Feng, J., Wehbi, H., and Roberts, M. F. (2002) J. Biol. Chem. 277, 19867-19875). The crystal structure supports this hypothesis. Relative to the crystal structure of the closely related (97% identity) wild-type PI-PLC from Bacillus cereus, significant conformational differences occur at the membrane-binding interfacial region rather than the active site. The Trp --> Ala mutations not only remove the membrane-partitioning aromatic side chains but also perturb the conformations of the so-called helix B and rim loop regions, both of which are implicated in interfacial binding. The crystal structure also reveals a homodimer, the first such observation for a bacterial PI-PLC, with pseudo-2-fold symmetry. The symmetric dimer interface is stabilized by hydrophobic and hydrogen-bonding interactions, contributed primarily by a central swath of aromatic residues arranged in a quasiherringbone pattern. Evidence that interfacially active wild-type PI-PLC enzymes may dimerize in the presence of phosphatidylcholine vesicles is provided by fluorescence quenching of PI-PLC mutants with pyrene-labeled cysteine residues. The combined data suggest that wild-type PI-PLC can form similar homodimers, anchored to the interface by the tryptophan and neighboring membrane-partitioning residues.
[Mh] Termos MeSH primário: Bacillus thuringiensis/enzimologia
Fosfatidilinositol Diacilglicerol-Liase/química
[Mh] Termos MeSH secundário: Cristalização
Cristalografia por Raios X
Dimerização
Ligações de Hidrogênio
Cinética
Modelos Moleculares
Conformação Molecular
Mutação
Fosfatidilinositol Diacilglicerol-Liase/metabolismo
Fosfoinositídeo Fosfolipase C
Ligação Proteica
Conformação Proteica
Estrutura Secundária de Proteína
Espectrometria de Fluorescência
Triptofano/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
8DUH1N11BX (Tryptophan); EC 3.1.4.11 (Phosphoinositide Phospholipase C); EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase)
[Em] Mês de entrada:0705
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070111
[St] Status:MEDLINE


  8 / 1403 MEDLINE  
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[PMID]:17169600
[Au] Autor:Chu KM; Chow KB; Leung PK; Lau PN; Chan CB; Cheng CH; Wise H
[Ad] Endereço:Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, SAR, China.
[Ti] Título:Over-expression of the truncated ghrelin receptor polypeptide attenuates the constitutive activation of phosphatidylinositol-specific phospholipase C by ghrelin receptors but has no effect on ghrelin-stimulated extracellular signal-regulated kinase 1/2 activity.
[So] Source:Int J Biochem Cell Biol;39(4):752-64, 2007.
[Is] ISSN:1357-2725
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In addition to regulating growth hormone release from the pituitary, ghrelin receptors also influence cell proliferation and apoptosis. By studying mitogen-activated protein kinase activity in human embryonic kidney 293 cells over-expressing ghrelin receptors, we aimed to identify the specific cell signalling pathways used by ghrelin receptors, and to determine if the truncated ghrelin receptor polypeptide had any influence on the functional activity of ghrelin receptors. We found that ghrelin activated extracellular signal-regulated kinases 1/2 with an EC50 value of 10 nM, and that this response was inhibited by the ghrelin receptor antagonists D-Lys3-GHRP-6 and [D-Arg1,D-Phe5,D-Trp(7,9),Leu11]-substance P. Ghrelin had little or no effect on the activity of c-Jun N-terminal kinase, p38 kinase or Akt. Ghrelin appeared to activate extracellular signal-regulated kinases 1/2 through a calcium-independent novel protein kinase C isoform which may utilize diacylglycerol derived from hydrolysis of phosphatidylcholine rather than from phosphatidylinositol. Ghrelin-stimulated extracellular signal-regulated kinases 1/2 activity was independent of transactivation of epidermal growth factor receptors, and even when ghrelin receptor internalization was blocked by concanavalin A or a beta-arrestin mutant, there was no decrease in phosphorylated extracellular signal-regulated kinases 1/2, suggesting this is a G protein-dependent process. The truncated ghrelin receptor polypeptide had no effect on ghrelin receptor signalling to extracellular signal-regulated kinases 1/2, but decreased the constitutive activation of phosphatidylinositol-specific phospholipase C by ghrelin receptors. In conclusion, our results suggest that any up-regulation of the truncated ghrelin receptor polypeptide might preferentially attenuate functional activity dependent on the constitutive activation of ghrelin receptors, while leaving ghrelin-dependent signalling unaffected.
[Mh] Termos MeSH primário: Proteína Quinase 1 Ativada por Mitógeno/metabolismo
Proteína Quinase 3 Ativada por Mitógeno/metabolismo
Hormônios Peptídicos/farmacologia
Fosfatidilinositol Diacilglicerol-Liase/metabolismo
Receptores Acoplados a Proteínas-G/metabolismo
[Mh] Termos MeSH secundário: Western Blotting
Linhagem Celular
Concanavalina A/farmacologia
Relação Dose-Resposta a Droga
Endocitose/efeitos dos fármacos
Ativação Enzimática/efeitos dos fármacos
Expressão Gênica
Grelina
Seres Humanos
Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo
Oligopeptídeos/farmacologia
Fosfoinositídeo Fosfolipase C
Fosforilação/efeitos dos fármacos
Isoformas de Proteínas/antagonistas & inibidores
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
Receptores Acoplados a Proteínas-G/antagonistas & inibidores
Receptores Acoplados a Proteínas-G/genética
Receptores de Grelina
Substância P/análogos & derivados
Substância P/farmacologia
Transfecção
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ghrelin); 0 (Oligopeptides); 0 (Peptide Hormones); 0 (Protein Isoforms); 0 (Receptors, G-Protein-Coupled); 0 (Receptors, Ghrelin); 11028-71-0 (Concanavalin A); 33507-63-0 (Substance P); 4H7N4I6X6A (growth hormone releasing hexapeptide); 96736-12-8 (substance P, Phe(5)-Trp(7,9)-Leu(11)-); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases); EC 3.1.4.11 (Phosphoinositide Phospholipase C); EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase)
[Em] Mês de entrada:0706
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:061216
[St] Status:MEDLINE


  9 / 1403 MEDLINE  
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[PMID]:17151916
[Au] Autor:Giussani P; Ferraretto A; Gravaghi C; Bassi R; Tettamanti G; Riboni L; Viani P
[Ad] Endereço:Department of Medical Chemistry, Biochemistry and Biotechnology, L.I.T.A. via F. Cervi 93, 20090 Segrate (Milan), Italy.
[Ti] Título:Sphingosine-1-phosphate and calcium signaling in cerebellar astrocytes and differentiated granule cells.
[So] Source:Neurochem Res;32(1):27-37, 2007 Jan.
[Is] ISSN:0364-3190
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:S1P is involved in the regulation of multiple biological processes (cell survival, growth, migration and differentiation) both in neurons and glial cells. The study was aimed at investigating the possible effects of S1P on calcium signaling in cerebellar astrocytes and differentiated granule cells. In cerebellar astrocytes S1P is able to mediate calcium signaling mainly through Gi protein coupled receptors, whereas in differentiated neurons it failed to evoke any calcium signaling, despite acting both extracellularly and intracellularly. The data indicate strict cell specificity in S1P-evoked calcium response, which could be relevant to communication between neurons and glial cells in the cerebellum.
[Mh] Termos MeSH primário: Astrócitos/fisiologia
Sinalização do Cálcio/efeitos dos fármacos
Lisofosfolipídeos/fisiologia
Esfingosina/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Sinalização do Cálcio/fisiologia
Diferenciação Celular
Células Cultivadas
Cerebelo/citologia
Fosfatidilinositol Diacilglicerol-Liase/metabolismo
Ratos
Esfingosina/farmacologia
Esfingosina/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lysophospholipids); 19794-97-9 (dihydrosphingosine 1-phosphate); 26993-30-6 (sphingosine 1-phosphate); EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase); NGZ37HRE42 (Sphingosine)
[Em] Mês de entrada:0703
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:061208
[St] Status:MEDLINE


  10 / 1403 MEDLINE  
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[PMID]:17142056
[Au] Autor:Cao Z; Zhang J; Li Y; Xu X; Liu G; Bhattacharrya MK; Yang H; Ren D
[Ad] Endereço:State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
[Ti] Título:Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits.
[So] Source:Protein Expr Purif;52(2):306-12, 2007 Apr.
[Is] ISSN:1046-5928
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phosphoinositide-specific phospholipase Cs (PI-PLCs) are important enzymes in eukaryotes, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate into the two second messengers inositol 1,4,5-trisphosphate and diacylglycerol. The Arabidopsis genome contains nine putative PI-PLC genes. AtPLC4, an abiotic stress induced gene, has been reported to encode an active PI-PLC isoform. However, the exact roles of putative AtPLC4 in plant remain to be elicited. The first 108 amino acid residues of the N-terminal of AtPLC4, referred to as AtPLC4 N, was expressed as a recombinant protein in Escherichia coli and used as antigen in generating antibody. Purified recombinant proteins including AtPLC1 to AtPLC5, AtPLC8, AtPLC9 and AtPLC4 N were transferred onto the same blot to test specificity of the prepared antibody. Western blot result shows that only AtPLC4 and AtPLC4 N can be recognized by the antibody. The antibody recognized a protein of approximately 68kDa in the plasma membrane fraction and cytosolic fractions prepared from Arabidopsis thaliana plants. This corresponds very well with the calculated molecular weight of AtPLC4. The results suggest that AtPLC4 may encode a plasma membrane-associated protein.
[Mh] Termos MeSH primário: Formação de Anticorpos
Arabidopsis/enzimologia
Sequência Conservada/imunologia
Fosfatidilinositol Diacilglicerol-Liase/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Especificidade de Anticorpos
Membrana Celular/enzimologia
Membrana Celular/imunologia
Citosol/enzimologia
Citosol/imunologia
Dados de Sequência Molecular
Fosfoinositídeo Fosfolipase C
Coelhos
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.1.4.11 (Phosphoinositide Phospholipase C); EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase)
[Em] Mês de entrada:0704
[Cu] Atualização por classe:071115
[Lr] Data última revisão:
071115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:061205
[St] Status:MEDLINE



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