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[PMID]:29251476
[Au] Autor:Singh G; Sharma P; Sharma S
[Ti] Título:Role of growth media on the phytopromotional potential of symbiotic fungus Piriformospoira indica.
[So] Source:J Environ Biol;37(5):889-94, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Piriformospora indica biomass generated in different growth media Rose Bengal medium, Kaefer's Medium, Enriched Soil Medium, Malt extract Medium and Czapek Dox was quantified and? their bioinoculum potential was compared using? moong bean (Vigna radiata) as? test plant. Plant chlorophyll content in response to inoculations was lowest in Rose Bengal medium (2.772mg plant-1) and highest due to inoculum produced on Enriched soil Medium (3.694 mg plant-1). The highest nitrogen content (19.260 mg plant-1) was recorded by inoculum produced on Kaefer's Medium followed by Enriched Soil Medium (19.123 mg plant-1), ME (18.19 mg plant-1) and CD medium (17.71 mg plant-1). The highest plant phosphorus uptake was registered in Enriched Soil Medium (17.153 mg plant-1) followed by Kaefer's Medium (17.023 mg plant-1). Maximum dry weight of plants was observed by inoculation with fungus cultured in Kaefer's Medium (3.416 g pot-1) and Enriched Soil Medium (3.349 g pot-1). Thus, growth medium used for the culture of fungus can influence its bioefficacy as plant growth promoting agent and Piriformospora indica can be grown on cost effective and simple mass multiplication medium which could augment its usage for commercial purposes.
[Mh] Termos MeSH primário: Basidiomycota/fisiologia
Endófitos
Fabaceae/crescimento & desenvolvimento
Fabaceae/microbiologia
[Mh] Termos MeSH secundário: Fosfatase Ácida/metabolismo
Fosfatase Alcalina/metabolismo
Regulação Enzimológica da Expressão Gênica/fisiologia
Regulação da Expressão Gênica de Plantas/fisiologia
Raízes de Plantas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.1.3.1 (Alkaline Phosphatase); EC 3.1.3.2 (Acid Phosphatase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


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[PMID]:28470516
[Au] Autor:Ivanov DP; Grabowska AM; Garnett MC
[Ad] Endereço:Cancer Biology, Division of Cancer and Stem Cells, School of Medicine, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK. delyan.ivanov@nottingham.ac.uk.
[Ti] Título:High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase Activity.
[So] Source:Methods Mol Biol;1601:43-59, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mainstream adoption of physiologically relevant three-dimensional models has been slow in the last 50 years due to long, manual protocols with poor reproducibility, high price, and closed commercial platforms. This chapter describes high-throughput, low-cost, open methods for spheroid viability assessment which use readily available reagents and open-source software to analyze spheroid volume, metabolism, and enzymatic activity. We provide two ImageJ macros for automated spheroid size determination-for both single images and images in stacks. We also share an Excel template spreadsheet allowing users to rapidly process spheroid size data, analyze plate uniformity (such as edge effects and systematic seeding errors), detect outliers, and calculate dose-response. The methods would be useful to researchers in preclinical and translational research planning to move away from simplistic monolayer studies and explore 3D spheroid screens for drug safety and efficacy without substantial investment in money or time.
[Mh] Termos MeSH primário: Sobrevivência Celular
Ensaios de Triagem em Larga Escala/métodos
Indicadores e Reagentes/metabolismo
Esferoides Celulares/fisiologia
[Mh] Termos MeSH secundário: Fosfatase Ácida/metabolismo
Encéfalo/citologia
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Avaliação Pré-Clínica de Medicamentos
Ensaios de Triagem em Larga Escala/economia
Seres Humanos
Processamento de Imagem Assistida por Computador
Oxazinas/química
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Software
Esferoides Celulares/citologia
Esferoides Celulares/metabolismo
Fatores de Tempo
Xantenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents); 0 (Oxazines); 0 (Xanthenes); 1FN9YD6968 (resazurin); EC 3.1.3.2 (Acid Phosphatase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_4


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[PMID]:29227075
[Au] Autor:Soni S; Basu M; Agrawal P; Kumar N; Bhatnagar A; Chhillar N
[Ti] Título:Multiple parametric approaches to assess acute radiation lung injury of rats radiation lung injury of rats.
[So] Source:Ukr Biochem J;88(1):22-30, 2016 Jan-Feb.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:The effect of whole body gamma irradiation (WBI) in single fraction was studied, as well as its influence on the secretion of various biochemical markers and cellular component that could be used as acute radiation lung injury marker. Sprague dawley rats were treated with WBI (60Co) of radiation dose from 1 Gy to 5 Gy (dose rate - 0.95 Gy/min). Bronchoalveolar lavage fluid was retrieved from all animals in control and radiation treated groups up to 72 h post radiation. Bronchoalveolar lavage fluid (BALF) was analyzed for lactate dehydrogenase (LDH ), acid phosphatase (AP ), alkaline phosphatase (ALP ), cell count and total protein. Intragroup and intergroup comparison of BALF parameters at different radiation doses showed significant difference. LDH was significantly increased as the dose increased from 1Gy to 5Gy (P = 0.00) after 2 h with effect size of difference (r > 0.3). ALP was significantly altered after 3Gy and 4Gy (P < 0.05). AP was significantly altered at 2Gy-5Gy (p < 0.05). Total protein level changed significantly from 1Gy to 5Gy (P < 0.00). Cellular content of BALF showed significant changes after radiation exposure. BALF parameters like LDH, AP, ALP, neutrophils, lymphocytes, total leukocyte count and total protein were sensitive to radiation exposure and their levels vary significantly up to 72 h after single whole body radiation exposure in Sprague dawley rats. It can be concluded that the biochemical indices in BALF have more wide application in evaluation of acute radiation induced lung injury.
[Mh] Termos MeSH primário: Raios gama/efeitos adversos
Lesão Pulmonar/patologia
Lesões Experimentais por Radiação/patologia
[Mh] Termos MeSH secundário: Fosfatase Ácida/imunologia
Fosfatase Ácida/metabolismo
Fosfatase Alcalina/imunologia
Fosfatase Alcalina/metabolismo
Animais
Biomarcadores/análise
Líquido da Lavagem Broncoalveolar/química
Líquido da Lavagem Broncoalveolar/citologia
Líquido da Lavagem Broncoalveolar/imunologia
Relação Dose-Resposta à Radiação
L-Lactato Desidrogenase/imunologia
L-Lactato Desidrogenase/metabolismo
Contagem de Leucócitos
Lesão Pulmonar/enzimologia
Lesão Pulmonar/imunologia
Linfócitos/imunologia
Linfócitos/patologia
Masculino
Neutrófilos/imunologia
Neutrófilos/patologia
Lesões Experimentais por Radiação/enzimologia
Lesões Experimentais por Radiação/imunologia
Ratos
Ratos Sprague-Dawley
Irradiação Corporal Total
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 3.1.3.1 (Alkaline Phosphatase); EC 3.1.3.2 (Acid Phosphatase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.01.022


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[PMID]:29253000
[Au] Autor:Lin S; Wang S; Si Y; Yang W; Zhu S; Ni W
[Ad] Endereço:College of Environmental and Resource Sciences, Zhejiang University, Key Laboratory of Agricultural Resource and Environment of Zhejiang Province, Hangzhou, P. R. China.
[Ti] Título:Variations in eco-enzymatic stoichiometric and microbial characteristics in paddy soil as affected by long-term integrated organic-inorganic fertilization.
[So] Source:PLoS One;12(12):e0189908, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To investigate the effects of different nutrient management regimes on the soil chemical, eco-enzymatic stoichiometric and microbial characteristics, soil samples were collected from a 30-year, long-term field experiment with six plots growing rice. The results showed that as integrated fertilization increased, so did the concentrations of soil total or available nutrients and microbial biomass carbon (MBC). Our results also found enhanced soil basal respiration and cumulative carbon mineralization compared to chemical fertilization alone at the same nutrient doses. The activities of soil protease (Pro), ß-glucosidase (ßG), N-acetyl-glucosaminidase (NAG) and acid phosphatase (AP) from the integrated fertilization treatments were significantly higher than those of the treatments without organic manure, so did the activities of soil leucyl aminopeptidase (LAP) and urease (Ure) from the treatment with organic manure in addition to farmer practise fertilization (NPKM2). The stoichiometric ratios, expressed as lnßG/ln(NAG+LAP)/lnPro/lnUre/lnAP, ranged from 1:0.94:1.04:0.67:1.01 to 1:0.98:1.10:0.78:1.25, indicating that the acquisition of C, N and P changed consistently and synchronously under different nutrient management strategies. Integrated fertilization was more beneficial to the acquisition and utilization of soil organic carbon compared to low-molecular-weight organic nitrogen. We concluded that protease and urease should be considered in eco-enzymatic stoichiometric assessments for the hydrolysis of proteins, amino acids, carbohydrates and phosphomonoesters in soil, and integrated fertilization with chemical fertilizers and organic manure should be recommended as a preferable nutrient management system for intensive rice cultivation.
[Mh] Termos MeSH primário: Agricultura/métodos
Carbono/química
Fertilizantes
Microbiologia do Solo
Solo/química
[Mh] Termos MeSH secundário: Acetilglucosaminidase/metabolismo
Fosfatase Ácida/metabolismo
Aminopeptidases/metabolismo
Biomassa
China
Esterco
Nitrogênio/química
Oryza
Fósforo/química
beta-Glucosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fertilizers); 0 (Manure); 0 (Soil); 27YLU75U4W (Phosphorus); 7440-44-0 (Carbon); EC 3.1.3.2 (Acid Phosphatase); EC 3.2.1.21 (beta-Glucosidase); EC 3.2.1.52 (Acetylglucosaminidase); EC 3.4.11.- (Aminopeptidases); N762921K75 (Nitrogen)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189908


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[PMID]:28813687
[Au] Autor:Harauchi Y; Kajimoto T; Ohta E; Kawachi H; Imamura-Jinda A; Ohta S
[Ad] Endereço:Graduate School of Biosphere Science, Hiroshima University, 1-7-1 Kagamiyama, Higashi-Hiroshima 739-8521, Japan.
[Ti] Título:Prenylated purine alkaloids from seeds of Gleditsia japonica.
[So] Source:Phytochemistry;143:145-150, 2017 Nov.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Three previously undescribed isoguanine glycosides with an N -prenyl group, designated locustoside B, saikachinoside B, and saikachinoside C, have been isolated from the seed of Gleditsia japonica Miquel (Fabaceae) along with two known compounds, locustoside A and saikachinoside A. Their structures were determined from spectroscopic data and X-ray crystallographic analysis. The inhibitory activity against acid phosphatase was evaluated.
[Mh] Termos MeSH primário: Gleditsia/química
Sementes/química
[Mh] Termos MeSH secundário: Fosfatase Ácida/antagonistas & inibidores
Alcaloides
Cristalografia por Raios X
Fabaceae/química
Glicosídeos/química
Japão
Conformação Molecular
Ressonância Magnética Nuclear Biomolecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Glycosides); EC 3.1.3.2 (Acid Phosphatase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170817
[St] Status:MEDLINE


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[PMID]:28719666
[Au] Autor:Luciano CS; Newell SJ
[Ad] Endereço:Department of Biology, Indiana University of Pennsylvania, Indiana, PA, United States of America.
[Ti] Título:Effects of prey, pitcher age, and microbes on acid phosphatase activity in fluid from pitchers of Sarracenia purpurea (Sarraceniaceae).
[So] Source:PLoS One;12(7):e0181252, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Carnivory in pitcher plants generally involves digestion of prey, by the plant itself, by symbionts, or both. While symbionts appear to be important in the digestion of prey in Sarracenia purpurea, the importance of pitcher-derived enzymes is less well documented. Our goal was to reduce microbial numbers in pitcher fluid in order to measure the acid phosphatase activity attributable to the pitchers themselves. Preliminary experiments indicated that various antibiotics were minimally effective at reducing microbial populations and that antibiotic-resistant microbes were easily cultured from pitcher fluid. Consequently, we measured the abundance of culturable microbes in every sample taken for the measurement of acid phosphatase activity. Pitchers fed with one sterilized ant had higher levels of acid phosphatase activity than unfed pitchers. Older pitchers were more responsive to feeding than young pitchers. Pitchers with high levels of microbes (on Day 5) had higher acid phosphatase activity than pitchers with low levels of microbes. However, fed pitchers were not more likely to have higher microbe levels and microbe levels were not related to pitcher age. When fluid samples from inside the pitcher were compared to appropriate controls incubated outside the pitcher, acid phosphatase activity was higher inside the pitcher. Results from the feeding experiments are consistent with a primary role of microbes in the digestion of prey in pitchers of S. purpurea. However, the relationship between pitcher age and enzyme activity is not a function of microbes in the pitcher fluid and may depend on enzymes produced by the plant. Our methods would not detect microbes embedded on the inner surface of the pitcher; and if they survived the alcohol rinse and antibiotics, we cannot rule out microbes as the source of the relationship between pitcher age and acid phosphatase activity.
[Mh] Termos MeSH primário: Fosfatase Ácida/metabolismo
Envelhecimento
Sarraceniaceae/enzimologia
Sarraceniaceae/microbiologia
[Mh] Termos MeSH secundário: Carnivoridade
Sarraceniaceae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.1.3.2 (Acid Phosphatase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181252


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[PMID]:28682542
[Au] Autor:Zhu C; Lingkai S
[Ad] Endereço:Dept. of Conservative Dentistry and Endodontics, Guiyang Hospital of Stomatology, Affiliated Guiyang Hospital of Stomatology, Zunyi Medical University, Guiyang 550002, China.
[Ti] Título:[Effects of paeonol on the function of bone marrow-derived macrophage from Porphyromonas gingivalis-induced mice].
[So] Source:Hua Xi Kou Qiang Yi Xue Za Zhi;35(2):139-144, 2017 Apr 01.
[Is] ISSN:1000-1182
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: This work aims to examine the effects of paeonol treatment on the ability of bone marrow-derived macrophage (BMM) to excrete inflammatory factors and to differentiate into osteoclasts upon induction with Porphyromonas gingivalis (P. gingivalis). This work also aims to investigate the underlying mechanisms of these abilities. METHODS: BMM culture was treated with different paeonol concentrations at for 1 h and then stimulated with P. gingivalis for 24 h before programmed death-ligand 1 (PD-L1) was quantified with flow cytometry. Tumor necrosis factor-α (TNF-α), interleukin (IL)-1ß, and IL-6 were detected by enzyme-linked immunosorbent assay (ELISA). The BMM culture was treated with the receptor activator for nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF), and then with paeonol for 1 h prior to induction with P. gingivalis. Then, osteoclast formation was assessed using tartrate resistant acid phosphatase (TRAP) staining. The osteoclast-related proteins TRAP and receptor activator of nuclear factor-κB (RANK) were quantified by Western blotting. RESULTS: Paeonol was nontoxic to BMM within a range of 10-50 µmol·L⁻¹. Flow cytometry showed that paeonol inhibited PD-L1 expression in P. gingivalis-induced BMM in a dose-dependent manner. ELISA indicated that paeonol dose-dependently inhibited the excretion of TNF-α, IL-1ß, and IL-6 by P. gingivalis-induced BMM (P<0.01). TRAP staining revealed that paenol treatment inhibited the differentiation of P. gingivalis-induced BMM into osteoclasts. Western blot results suggested that paeonol decreased the expression of TRAP and RANK in BMM. CONCLUSIONS: Paeonol dose-dependently inhibited the excretion of the inflammatory factors TNF-α, IL-1ß, and IL-6 by P. gingivalis-induced BMM in a dose-dependent manner. Moreover, paenol treatment prevented the differentiation of P. gingivalis-induced BMM differentiation into osteoclasts.
.
[Mh] Termos MeSH primário: Acetofenonas/farmacologia
Diferenciação Celular
Macrófagos
Osteoclastos
Porphyromonas gingivalis
[Mh] Termos MeSH secundário: Fosfatase Ácida
Animais
Proteínas de Transporte
Interleucina-1beta
Interleucina-6
Isoenzimas
Fator Estimulador de Colônias de Macrófagos
Glicoproteínas de Membrana
Camundongos
Ligante RANK
Receptor Ativador de Fator Nuclear kappa-B
Fator de Necrose Tumoral alfa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetophenones); 0 (Carrier Proteins); 0 (Interleukin-1beta); 0 (Interleukin-6); 0 (Isoenzymes); 0 (Membrane Glycoproteins); 0 (RANK Ligand); 0 (Receptor Activator of Nuclear Factor-kappa B); 0 (Tumor Necrosis Factor-alpha); 3R834EPI82 (paeonol); 81627-83-0 (Macrophage Colony-Stimulating Factor); EC 3.1.3.2 (Acid Phosphatase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.7518/hxkq.2017.02.006


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[PMID]:28605649
[Au] Autor:Thanakiatkrai P; Raham K; Pradutkanchana J; Sotthibandhu S; Kitpipit T
[Ad] Endereço:Forensic Science Program, Department of Applied Science, Faculty of Science, Prince of Songkla University, Thailand.
[Ti] Título:Direct-STR typing from presumptively-tested and untreated body fluids.
[So] Source:Forensic Sci Int Genet;30:1-9, 2017 Sep.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Body fluids provide key pieces of information for a forensic investigation. However, sometimes only a small amount of body fluids is found and/or DNA are also degraded by environmental factors at the crime scene. In extreme cases, a forensic analyst may have to decide whether to perform a presumptive test on the stains or proceed straightaway to DNA profiling, which could be wasteful for non-biological stains. Additionally, due to the inefficient DNA extraction process, the amount of DNA may not be enough for STR typing, especially if parts of the evidence had been subjected to presumptive testing. To overcome these problems, we developed a direct PCR method for STR profiling of stains (blood, saliva, and semen) that had been subjected to presumptive tests and also those that had not undergone presumptive tests. Using the optimized protocols, 86 of 90 untreated samples (95.6%) resulted in a full DNA profile. For presumptively-tested samples, both the type of presumptive test used and the surfaces where the stains are deposited affected the quality of the STR profiles. With blood, we obtained full STR profiles from 88% of samples tested with luminol and 78% with Hemastix. The acid phosphatase test for semen and Phadebas test for saliva resulted in full STR profiles from 85% and 73% of samples, respectively. Different substrates also affected the resulting STR profiles, but there was no clear trend based on absorbency or texture. The interactions of types of body fluids, presumptive tests, and substrates must be considered together. Our direct PCR protocol can be used to detect DNA even with 6 months-old biological samples. The benefits of the developed protocol include increasing amount of DNA obtained from evidence, decreasing chances of DNA contamination from complex or lengthy extraction steps, using minimal sample amount for analysis, and most importantly, improving STR profiles. Also, the process could save analysis time and cost due to the omission of DNA extraction and quantification. Our developed method could be beneficial to cases with limited stains available, as forensic analysts can perform indirect presumptive testing on the suspected stains and direct PCR could be carried out from the filter paper used, thus leaving the original stain for subsequent DNA extraction or re-analysis.
[Mh] Termos MeSH primário: Análise Química do Sangue
Impressões Digitais de DNA/métodos
Repetições de Microssatélites
Saliva/química
Sêmen/química
[Mh] Termos MeSH secundário: Fosfatase Ácida
Seres Humanos
Luminol
Masculino
Reação em Cadeia da Polimerase
Fitas Reagentes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Reagent Strips); 5EXP385Q4F (Luminol); EC 3.1.3.2 (Acid Phosphatase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE


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[PMID]:28605459
[Au] Autor:Pettersson M; Viljakainen H; Loid P; Mustila T; Pekkinen M; Armenio M; Andersson-Assarsson JC; Mäkitie O; Lindstrand A
[Ad] Endereço:Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm 171 77, Sweden.
[Ti] Título:Copy Number Variants Are Enriched in Individuals With Early-Onset Obesity and Highlight Novel Pathogenic Pathways.
[So] Source:J Clin Endocrinol Metab;102(8):3029-3039, 2017 Aug 01.
[Is] ISSN:1945-7197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Context: Only a few genetic causes for childhood obesity have been identified to date. Copy number variants (CNVs) are known to contribute to obesity, both syndromic (15q11.2 deletions, Prader-Willi syndrome) and nonsyndromic (16p11.2 deletions) obesity. Objective: To study the contribution of CNVs to early-onset obesity and evaluate the expression of candidate genes in subcutaneous adipose tissue. Design and Setting: A case-control study in a tertiary academic center. Participants: CNV analysis was performed on 90 subjects with early-onset obesity and 67 normal-weight controls. Subcutaneous adipose tissue from body mass index-discordant siblings was used for the gene expression analyses. Main Outcome Measures: We used custom high-density array comparative genomic hybridization with exon resolution in 1989 genes, including all known obesity loci. The expression of candidate genes was assessed using microarray analysis of messenger RNA from subcutaneous adipose tissue. Results: We identified rare CNVs in 17 subjects (19%) with obesity and 2 controls (3%). In three cases (3%), the identified variant involved a known syndromic lesion (22q11.21 duplication, 1q21.1 deletion, and 16p11.2 deletion, respectively), although the others were not known. Seven CNVs in 10 families were inherited and segregated with obesity. Expression analysis of 37 candidate genes showed discordant expression for 10 genes (PCM1, EFEMP1, MAMLD1, ACP6, BAZ2B, SORBS1, KLF15, MACROD2, ATR, and MBD5). Conclusions: Rare CNVs contribute possibly pathogenic alleles to a substantial fraction of children with early-onset obesity. The involved genes might provide insights into pathogenic mechanisms and involved cellular pathways. These findings highlight the importance of CNV screening in children with early-onset obesity.
[Mh] Termos MeSH primário: Obesidade Pediátrica/genética
RNA Mensageiro/metabolismo
Gordura Subcutânea/metabolismo
[Mh] Termos MeSH secundário: Anormalidades Múltiplas/genética
Fosfatase Ácida/genética
Adolescente
Adulto
Proteínas Mutadas de Ataxia Telangiectasia/genética
Transtorno Autístico/genética
Autoantígenos/genética
Estudos de Casos e Controles
Proteínas de Ciclo Celular/genética
Criança
Pré-Escolar
Deleção Cromossômica
Transtornos Cromossômicos/genética
Duplicação Cromossômica/genética
Cromossomos Humanos Par 1/genética
Cromossomos Humanos Par 16/genética
Cromossomos Humanos Par 22/genética
Hibridização Genômica Comparativa
Variações do Número de Cópias de DNA
Enzimas Reparadoras do DNA/genética
Proteínas de Ligação a DNA/genética
Síndrome de DiGeorge/genética
Proteínas da Matriz Extracelular/genética
Feminino
Seres Humanos
Hidrolases/genética
Deficiência Intelectual/genética
Fatores de Transcrição Kruppel-Like/genética
Masculino
Megalencefalia/genética
Proteínas dos Microfilamentos/genética
Proteínas Nucleares/genética
Proteínas/genética
Irmãos
Fatores de Transcrição/genética
Transcriptoma
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Autoantigens); 0 (BAZ2B protein, human); 0 (Cell Cycle Proteins); 0 (DNA-Binding Proteins); 0 (EFEMP1 protein, human); 0 (Extracellular Matrix Proteins); 0 (KLF15 protein, human); 0 (Kruppel-Like Transcription Factors); 0 (MACROD2 protein, human); 0 (MAMLD1 protein, human); 0 (MBD5 protein, human); 0 (Microfilament Proteins); 0 (Nuclear Proteins); 0 (PCM1 protein, human); 0 (Proteins); 0 (RNA, Messenger); 0 (SORBS1 protein, human); 0 (Transcription Factors); EC 2.7.11.1 (ATR protein, human); EC 2.7.11.1 (Ataxia Telangiectasia Mutated Proteins); EC 3.- (Hydrolases); EC 3.1.3.2 (Acid Phosphatase); EC 3.1.3.2 (acid phosphatase-like protein 1, human); EC 6.5.1.- (DNA Repair Enzymes)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE
[do] DOI:10.1210/jc.2017-00565


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[PMID]:28600898
[Au] Autor:Tasnádi G; Zechner M; Hall M; Baldenius K; Ditrich K; Faber K
[Ad] Endereço:Austrian Centre of Industrial Biotechnology, c/o.
[Ti] Título:Investigation of acid phosphatase variants for the synthesis of phosphate monoesters.
[So] Source:Biotechnol Bioeng;114(10):2187-2195, 2017 Oct.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The major drawback of using phosphatases for transphosphorylation reactions lies in product depletion caused by the natural hydrolytic activity of the enzymes. Variants of PhoC-Mm from Morganella morganii and NSAP-Eb from Escherichia blattae were studied for their ability to maintain a high product level in the transphosphorylation of various primary alcohols. A single amino acid exchange delivered phosphatase variant PhoC-Mm G92D, which was able to catalyze the phosphorylation of primary alcohols without any major hydrolysis of the formed phosphate esters. The mutation mostly improved the affinity of the enzyme for alcohols, while rate constants of transphosphorylation and hydrolysis were decreased, overall resulting in a superior catalytic efficiency in transphosphorylation compared to hydrolysis. The presence of residual substrate alcohol at a given concentration was crucial to suppress phosphate ester hydrolysis. The present work extends the synthetic applicability of phosphatase variants beyond the previously reported nucleosides and allows preparative-scale production of various primary phosphate esters (yields up to 42%) with high enzyme productivity (TONs up to ∼66,000). Biotechnol. Bioeng. 2017;114: 2187-2195. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Fosfatase Ácida/química
Álcoois/química
Escherichia/enzimologia
Ésteres/síntese química
Morganella morganii/enzimologia
Fosfatos/síntese química
[Mh] Termos MeSH secundário: Fosfatase Ácida/genética
Ativação Enzimática
Mutagênese Sítio-Dirigida
Fosforilação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alcohols); 0 (Esters); 0 (Phosphates); EC 3.1.3.2 (Acid Phosphatase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170611
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26352



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