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  1 / 1376 MEDLINE  
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[PMID]:29373588
[Au] Autor:Muszynski S; Tomaszewska E; Kwiecien M; Dobrowolski P; Tomczyk-Warunek A
[Ad] Endereço:Department of Physics, Faculty of Production Engineering, University of Life Sciences in Lublin, Lublin, Poland.
[Ti] Título:Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens.
[So] Source:PLoS One;13(1):e0191964, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Zinc is required for normal bone development and cartilage formation. The purpose of this study was to assess the effect of with adding organic Zn (alone or phytase inclusion) at the reduced dose to growing male Ross 308 chickens on somatic axis and bone tissue metabolism. 200 one-day old broilers were divided into the negative control group fed diet without Zn or phytase inclusion, positive control group receiving Zn in the 100% of daily recommended dose from ZnO, and two experimental groups fed diet introduced Zn in 25% of daily recommendation as a glycine chelate (Zn-Gly) with or without phytase inclusion (500 FTU·kg-1). Supplemental organic Zn increased bone Zn and Mg content, serum IGF-1, growth hormone and leptin concentration. Additional phytase inclusion increased body weight gain, blood plasma Ca, Fe, Zn and osteocalcin concentration and tibia ash percentage when compared to the Zn-deprived control. Bone geometry, yield and ultimate strengths were enhanced in both organic Zn supplemented groups, and the overall mechanical strength parameters of bone were better in these groups than in the positive control group supplemented with standard dose of inorganic Zn. Also marked improvements in the thickness of articular and the growth plate cartilages as well as real bone volume and thickness of metaphyseal trabeculae were achieved in all broilers fed Zn-supplemented diet irrespective of phytase inclusion, however, the highest cancellous bone mass and the best trabecular structure were noted after ZnO supplementation. In concludion, although dietary organic Zn given to growing broilers in 25% of daily recommended dose improved general bone properties and mechanical strength, the obtained results do not allow to unambiguously state that organic Zn supplementation at this level, even after phytase inclusion, is sufficient for proper bone development.
[Mh] Termos MeSH primário: 6-Fitase/metabolismo
Osso e Ossos/metabolismo
Zinco/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Galinhas
Hormônio do Crescimento/metabolismo
Fator de Crescimento Insulin-Like I/metabolismo
Leptina/metabolismo
Magnésio/metabolismo
Masculino
Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Leptin); 67763-96-6 (Insulin-Like Growth Factor I); 9002-72-6 (Growth Hormone); EC 3.1.3.26 (6-Phytase); I38ZP9992A (Magnesium); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191964


  2 / 1376 MEDLINE  
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[PMID]:29194015
[Au] Autor:Tripathi P; A JL; Kapoor M
[Ad] Endereço:a Department of Protein Chemistry and Technology , CSIR-Central Food Technological Research Institute , Mysuru , Karnataka , India.
[Ti] Título:Phytase from Citrobacter koseri PM-7: Enhanced production using statistical method and application in ameliorating mineral bioaccessibility and protein digestibility of high-phytate food.
[So] Source:Prep Biochem Biotechnol;48(1):84-91, 2018 Jan 02.
[Is] ISSN:1532-2297
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study was aimed at enhancing phytase (Phy-Ck) production from Citrobacter koseri PM-7 using response surface methodology (RSM) and improving the bioaccessibility of minerals (Fe and Zn) and protein digestibility in high-phytate food using Phy-Ck. A five-variable and three-level central composite design of RSM using wheat bran (6.681%, w/v), inoculum level (2.5%, v/v), and triton X-100 (0.2%, v/v) resulted in up to 5.57-fold (1.047 U/ml) improvement in Phy-Ck yield from C. koseri PM-7 when compared with fermentation media I and II. The model was successfully validated in the design space by taking a random set of variable combinations. Treatment of high-phytate food with partially purified Phy-Ck showed improvement in mineral bioaccessibility maximally for defatted sesame flour (DSF) (Fe 45.5%; Zn 50.7%) followed by wheat flour (WF) (Fe 13.5%; Zn 14.4%), green gram flour (GGF) (Fe 0.7%; Zn 3.8%) and defatted groundnut flour (DGF) (Zn 5.6%). The in vitro protein digestibility (IVPD) of WF increased from 48.83 to 65.04%, GGF from 45.04 to 57.12%, and DSF from 47.34 to 55.7% after Phy-Ck treatment.
[Mh] Termos MeSH primário: 6-Fitase/metabolismo
Citrobacter koseri/enzimologia
[Mh] Termos MeSH secundário: Ração Animal/análise
Fibras na Dieta/análise
Fermentação
Farinha/análise
Ferro/metabolismo
Ácido Fítico/análise
Ácido Fítico/metabolismo
Proteólise
Triticum/química
Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fiber); 7IGF0S7R8I (Phytic Acid); E1UOL152H7 (Iron); EC 3.1.3.26 (6-Phytase); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1080/10826068.2017.1405024


  3 / 1376 MEDLINE  
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[PMID]:28741666
[Au] Autor:Farhat-Khemakhem A; Blibech M; Boukhris I; Makni M; Chouayekh H
[Ad] Endereço:Laboratoire de Microorganismes et de Biomolécules, Centre de Biotechnologie de Sfax, Université de Sfax, BP, 1177, 3018, Sfax, Tunisia.
[Ti] Título:Assessment of the potential of the multi-enzyme producer Bacillus amyloliquefaciens US573 as alternative feed additive.
[So] Source:J Sci Food Agric;98(3):1208-1215, 2018 Feb.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Recently, probiotics have increasingly been used as feed additives in poultry diets as an alternative to antibiotic growth promoters fostering resistance development. RESULTS: This study was aimed at assessing the potential of Bacillus amyloliquefaciens US573 as a direct-fed microbial. The US573 strain was found to be free of harmful enzymatic activities and sensitive to antibiotics. In addition, it showed a good acid and bovine bile tolerance, high adhesion efficacy to chicken enterocytes, and an ability to form biofilms, which may favor its survival and persistence in the animal gastrointestinal tract. Moreover, besides the previously described extremely salt-tolerant and highly thermostable phytase, the US573 strain secretes xylanase, ß-glucanase and amylase activities useful in neutralizing antinutritional factors and maximizing the absorption of nutrients. The secretion of such enzymes may be responsible for the good performance of the US573 isolate in the digestibility of wheat in vitro. Indeed, using the vegetative cells, a yield of wheat dry matter digestibility of approximately 48% was achieved, which is slightly lower than the commercial feed additive Rovabio used as a reference (56.73% digestibility). CONCLUSION: The obtained results illustrate the potential of US573 strain as a promising direct-fed microbial candidate for application in the poultry industry. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Ração Animal/análise
Bacillus amyloliquefaciens/química
Bacillus amyloliquefaciens/enzimologia
Suplementos Nutricionais/análise
Probióticos/análise
[Mh] Termos MeSH secundário: 6-Fitase/química
6-Fitase/metabolismo
Amilases/química
Amilases/metabolismo
Animais
Antibacterianos/farmacologia
Bacillus amyloliquefaciens/efeitos dos fármacos
Bacillus amyloliquefaciens/fisiologia
Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Biofilmes
Bovinos
Galinhas
Digestão
Estabilidade Enzimática
Concentração de Íons de Hidrogênio
Probióticos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); EC 3.1.3.26 (6-Phytase); EC 3.2.1.- (Amylases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8574


  4 / 1376 MEDLINE  
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[PMID]:28468891
[Au] Autor:Monnard A; Moretti D; Zeder C; Steingötter A; Zimmermann MB
[Ad] Endereço:Department of Health Sciences and Technology, Laboratory of Human Nutrition, ETH Zürich, Zurich, Switzerland; and.
[Ti] Título:The effect of lipids, a lipid-rich ready-to-use therapeutic food, or a phytase on iron absorption from maize-based meals fortified with micronutrient powders.
[So] Source:Am J Clin Nutr;105(6):1521-1527, 2017 Jun.
[Is] ISSN:1938-3207
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ready-to-use-therapeutic foods (RUTFs) high in lipid, protein, and iron are used to treat malnutrition. Lipids increase gastric residence time, which could increase iron absorption, particularly from poorly soluble iron compounds and in combination with phytase. The objectives were to ) assess the effect on iron absorption of a lipid emulsion given 20 min before or together with an iron-fortified maize meal and ) assess iron absorption from a micronutrient powder (MNP) given with a nutrient-dense RUTF and/or a microbial phytase. A total of 41 women participated in 3 studies. They consumed a maize meal fortified with isotopically labeled ferrous sulfate (FeSO ; study 1) or ferric pyrophosphate (FePP; study 2). In studies 1 and 2, a lipid emulsion was given with or 20 min before the meal. In study 3, with the use of a 2 × 2 factorial design, subjects consumed a maize meal fortified with an MNP containing labeled FeSO (MNP) given with an RUTF (MNP+RUTF), with a phytase (MNP+phytase), or both (MNP+RUTF+phytase). Iron absorption was assessed by isotope incorporation in erythrocytes 14 d after the test meals. The lipid emulsion given either before or with the meal significantly increased iron absorption from FePP by 2.55-fold (95% CI: 1.48-, 4.37-fold; = 0.001) but not from FeSO There was a trend to increase iron absorption with the MNP+RUTF meal, which did not reach significance (1.21-fold; 95% CI: 0.92-, 1.61-fold; = 0.060). The addition of phytase to MNP and MNP+RUTF significantly increased iron absorption by 1.85-fold (95% CI: 1.49-, 2.29-fold; < 0.001), with no interaction between phytase and RUTF. In iron-fortified maize-based meals, the addition of lipids more than doubles iron absorption from FePP. Our results suggest the possibility of an enhancing effect on iron absorption of lipid-rich RUTFs, but more research is needed to determine this. This trial was registered at clinicaltrials.gov as NCT01991626.
[Mh] Termos MeSH primário: 6-Fitase/farmacologia
Alimentos Fortificados
Absorção Intestinal/efeitos dos fármacos
Ferro na Dieta/sangue
Ferro/sangue
Lipídeos/farmacologia
Micronutrientes/sangue
[Mh] Termos MeSH secundário: Adulto
Suplementos Nutricionais
Difosfatos/sangue
Eritrócitos/metabolismo
Feminino
Ferritinas/sangue
Seres Humanos
Refeições
Pós
Adulto Jovem
Zea mays
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Diphosphates); 0 (Iron, Dietary); 0 (Lipids); 0 (Micronutrients); 0 (Powders); 9007-73-2 (Ferritins); E1UOL152H7 (Iron); EC 3.1.3.26 (6-Phytase); QK8899250F (ferric pyrophosphate)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180121
[Lr] Data última revisão:
180121
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170505
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE
[do] DOI:10.3945/ajcn.116.142976


  5 / 1376 MEDLINE  
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[PMID]:29216308
[Au] Autor:Tsang PW; Fong WP; Samaranayake LP
[Ad] Endereço:Technological and Higher Education Institute of Hong Kong, HKSAR, China.
[Ti] Título:Candida albicans orf19.3727 encodes phytase activity and is essential for human tissue damage.
[So] Source:PLoS One;12(12):e0189219, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Candida albicans is a clinically important human fungal pathogen. We previously identified the presence of cell-associated phytase activity in C. albicans. Here, we reveal for the first time, that orf19.3727 contributes to phytase activity in C. albicans and ultimately to its virulence potency. Compared with its wild type counterpart, disruption of C. albicans orf19.3727 led to decreased phytase activity, reduced ability to form hyphae, attenuated in vitro adhesion, and reduced ability to penetrate human epithelium, which are the major virulence attributes of this yeast. Thus, orf19.3727 of C. albicans plays a key role in fungal pathogenesis. Further, our data uncover a putative novel strategy for anti-Candidal drug design through inhibition of phytase activity of this common pathogen.
[Mh] Termos MeSH primário: 6-Fitase/metabolismo
Candida albicans/genética
[Mh] Termos MeSH secundário: Candida albicans/enzimologia
Candida albicans/patogenicidade
Fases de Leitura Aberta
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.1.3.26 (6-Phytase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189219


  6 / 1376 MEDLINE  
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[PMID]:29173209
[Au] Autor:Cowieson AJ; Roos FF; Ruckebusch JP; Wilson JW; Guggenbuhl P; Lu H; Ajuwon KM; Adeola O
[Ad] Endereço:1DSM Nutritional Products,Wurmisweg 576,4303 Kaiseraugst,Switzerland.
[Ti] Título:Time-series responses of swine plasma metabolites to ingestion of diets containing myo-inositol or phytase.
[So] Source:Br J Nutr;118(11):897-905, 2017 Dec.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effect of the ingestion of diets containing either myo-inositol or exogenous phytase on plasma metabolites was examined using 29 kg barrows. The diets were: control (maize, soya, rapeseed, rice bran), control plus 2 g/kg myo-inositol, control plus 1000 phytase units (FYT)/kg or 3000 FYT/kg exogenous phytase. Pigs were housed in a PigTurn device and blood was collected, from jugular catheters, via an automated system at -30, (30 min before feeding), 0, 15, 30, 45, 60, 90, 120, 150, 180, 240, 300 and 360 min post-feeding. The addition of 2 g/kg myo-inositol to the basal diet resulted in an increase in plasma myo-inositol concentration that was evident 45-60 min after diet introduction and persisted to 360 min post-feeding. Similarly, supplementation of the basal diet with either 1000 or 3000 FYT/kg exogenous phytase resulted in an increase in plasma myo-inositol concentration that was still rising 360 min post-feeding. Plasma P concentration was increased over time by the addition of 1000 and 3000 FYT/kg phytase, but not by the addition of myo-inositol. Other plasma metabolites examined were not affected by dietary treatment. It can be concluded that oral delivery of myo-inositol results in rapid increase in plasma myo-inositol concentrations that peak approximately 45-60 min after feeding. Use of supplemental phytase achieves similar increases in myo-inositol concentration in plasma but the appearance is more gradual. Furthermore, supplementation of pig diets with exogenous phytase results in rapid appearance of P in plasma that may be sustained over time relative to diets with no added phytase.
[Mh] Termos MeSH primário: 6-Fitase/administração & dosagem
Fenômenos Fisiológicos da Nutrição Animal
Dieta/veterinária
Inositol/administração & dosagem
[Mh] Termos MeSH secundário: 6-Fitase/sangue
Fosfatase Alcalina/sangue
Ração Animal
Animais
Brassica rapa
Fibras na Dieta/administração & dosagem
Suplementos Nutricionais
Inositol/sangue
Feijão de Soja
Suínos
Zea mays
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fiber); 4L6452S749 (Inositol); EC 3.1.3.1 (Alkaline Phosphatase); EC 3.1.3.26 (6-Phytase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171218
[Lr] Data última revisão:
171218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517003026


  7 / 1376 MEDLINE  
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[PMID]:28752758
[Au] Autor:Niu C; Yang P; Luo H; Huang H; Wang Y; Yao B
[Ad] Endereço:National Engineering Research Center of Biological Feed, Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences , Beijing 100081, People's Republic of China.
[Ti] Título:Engineering of Yersinia Phytases to Improve Pepsin and Trypsin Resistance and Thermostability and Application Potential in the Food and Feed Industry.
[So] Source:J Agric Food Chem;65(34):7337-7344, 2017 Aug 30.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Susceptibility to proteases usually limits the application of phytase. We sought to improve the pepsin and trypsin resistance of YeAPPA from Yersinia enterocolitica and YkAPPA from Y. kristensenii by optimizing amino acid polarity and charge. The predicted pepsin/trypsin cleavage sites F89/K226 in pepsin/trypsin-sensitive YeAPPA and the corresponding sites (F89/E226) in pepsin-sensitive but trypsin-resistant YkAPPA were substituted with S and H, respectively. Six variants were produced in Pichia pastoris for catalytic and biochemical characterization. F89S, E226H, and F89S/E226H elevated pepsin resistance and thermostability and K226H and F89S/K226H improved pepsin and trypsin resistance and stability at 60 °C and low pH. All the variants increased the ability of the proteins to hydrolyze phytate in corn meal by 2.6-14.9-fold in the presence of pepsin at 37 °C and low pH. This study developed a genetic manipulation strategy specific for pepsin/trypsin-sensitive phytases that can improve enzyme tolerance against proteases and heat and benefit the food and feed industry in a cost-effective way.
[Mh] Termos MeSH primário: 6-Fitase/química
Proteínas de Bactérias/química
Yersinia/enzimologia
[Mh] Termos MeSH secundário: 6-Fitase/genética
6-Fitase/metabolismo
Ração Animal/análise
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Estabilidade Enzimática
Aditivos Alimentares/química
Aditivos Alimentares/metabolismo
Temperatura Alta
Concentração de Íons de Hidrogênio
Hidrólise
Pepsina A/química
Engenharia de Proteínas
Tripsina/química
Yersinia/química
Yersinia/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Food Additives); EC 3.1.3.26 (6-Phytase); EC 3.4.21.4 (Trypsin); EC 3.4.23.1 (Pepsin A)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170729
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02116


  8 / 1376 MEDLINE  
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[PMID]:28472144
[Au] Autor:Bekalu ZE; Madsen CK; Dionisio G; Brinch-Pedersen H
[Ad] Endereço:Department of Molecular Biology and Genetics, Research Center Flakkebjerg, Aarhus University, Slagelse, Denmark.
[Ti] Título:Aspergillus ficuum phytase activity is inhibited by cereal grain components.
[So] Source:PLoS One;12(5):e0176838, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the current study, we report for the first time that grain components of barley, rice, wheat and maize can inhibit the activity of Aspergillus ficuum phytase. The phytase inhibition is dose dependent and varies significantly between cereal species, between cultivars of barley and cultivars of wheat and between Fusarium graminearum infected and non-infected wheat grains. The highest endpoint level of phytase activity inhibition was 90%, observed with grain protein extracts (GPE) from F. graminearum infected wheat. Wheat GPE from grains infected with F. graminearum inhibits phytase activity significantly more than GPE from non-infected grains. For four barley cultivars studied, the IC50 value ranged from 0.978 ± 0.271 to 3.616 ± 0.087 mg×ml-1. For two non-infected wheat cultivars investigated, the IC50 values were varying from 2.478 ± 0.114 to 3.038 ± 0.097 mg×ml-1. The maize and rice cultivars tested gaveIC50 values on 0.983 ± 0.205 and 1.972 ± 0.019 mg×ml-1, respectively. After purifying the inhibitor from barley grains via Superdex G200, an approximately 30-35 kDa protein was identified. No clear trend for the mechanism of inhibition could be identified via Michaelis-Menten kinetics and Lineweaver-Burk plots. However, testing of the purified phytase inhibitor together with the A. ficuum phytase and the specific protease inhibitors pepstatin A, E64, EDTA and PMSF revealed that pepstatin A repealed the phytase inhibition. This indicates that the observed inhibition of A. ficuum phytase by cereal grain extracts is caused by protease activity of the aspartic proteinase type.
[Mh] Termos MeSH primário: 6-Fitase/antagonistas & inibidores
Aspergillus/enzimologia
Inibidores Enzimáticos/farmacologia
Hordeum/química
Triticum/química
[Mh] Termos MeSH secundário: Cromatografia em Gel
Cinética
Extratos Vegetais/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Plant Extracts); EC 3.1.3.26 (6-Phytase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0176838


  9 / 1376 MEDLINE  
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[PMID]:28384593
[Au] Autor:Makolomakwa M; Puri AK; Permaul K; Singh S
[Ad] Endereço:Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, Durban 4000, South Africa.
[Ti] Título:Thermo-acid-stable phytase-mediated enhancement of bioethanol production using Colocasia esculenta.
[So] Source:Bioresour Technol;235:396-404, 2017 Jul.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytase production by the thermophilic mould Thermomyces lanuginosus SSBP was enhanced 8.56-fold in submerged fermentation, which was further improved in fed-batch cultivations. The protein was purified to homogeneity using ammonium sulphate precipitation, Resource Q anion exchange and Superdex gel-filtration chromatography, with an overall purification of 24.7-fold and a yield of 5.16%. The purified 49kDa protein was optimally active at 55°C and pH 5.0, and was stable between 50 and 90°C from pH 3.0-6.0, with a half-life of 138.6min at 70°C. It was moderately stimulated by Ba and Mg . The enzyme reduced phytate content in Colocasia esculenta starch (from 1.43mg/g to 0.05mg/g) that resulted in an improvement in the availability of fermentable sugars with a concomitant reduction in viscosity and 1.59-fold improvement in ethanol production. Thermo-acid-stable phytase from T. lanuginosus SSBP could be of major biotechnological interest, especially due to its robustness and wide applicability.
[Mh] Termos MeSH primário: 6-Fitase/biossíntese
Colocasia
Etanol/metabolismo
[Mh] Termos MeSH secundário: Fermentação
Meia-Vida
Concentração de Íons de Hidrogênio
Ácido Fítico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3K9958V90M (Ethanol); 7IGF0S7R8I (Phytic Acid); EC 3.1.3.26 (6-Phytase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE


  10 / 1376 MEDLINE  
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[PMID]:28380608
[Au] Autor:Wu D; Wu SB; Choct M; Swick RA
[Ti] Título:Performance, intestinal microflora, and amino acid digestibility altered by exogenous enzymes in broilers fed wheat- or sorghum-based diets.
[So] Source:J Anim Sci;95(2):740-751, 2017 Feb.
[Is] ISSN:1525-3163
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of the current study was to compare the effects of dietary enzymes and nutrient restriction on performance and bone mineralization in birds fed wheat- or sorghum-based diets. A total of 720 d-old male broiler chicks were randomly allocated to 8 treatments, with 6 replicates per treatment and 15 birds per replicate. Birds were reared in floor pens from 0 to 35 d. The study used a 2 × 4 factorial arrangement with 2 grains (sorghum or wheat) and 4 diets: positive control (no enzyme and ME, digestible Lys, Ca, and P sufficient, negative control (NC; no enzyme and reduced ME [-100 kcal/kg], digestible AA [-2%], Ca [-0.12 percentage points], and available P [-0.18 percentage points in the starter phase and -0.22 percentage points in the grower phase]), NC + nonstarch polysaccharide-degrading enzymes + phytase (500 phytase units [FTU]; NCCP), and NC + phytase (1,000 FTU; NCP). From 0 to 35 d, birds fed wheat-based diets had greater G:F (4.5%), BW gain (9.2%), breast meat yield (6.8%), and tibia ash (2.0%) compared with birds fed sorghum-based diets. Across grain types, the NCCP treatment improved BW gain ( < 0.001), feed intake ( < 0.001), G:F ( < 0.05), and livability ( < 0.001) compared with the NC treatment. Birds fed NCP had greater BW gain ( < 0.001), feed intake ( < 0.001), G:F ( < 0.001), and livability ( < 0.001) compared with birds fed NC. Birds fed the NCP diet had greater BW gain ( < 0.001), toe ash ( < 0.01), and tibia ash ( < 0.001) compared with birds fed the NCCP diet. There was a grain × diet interaction for feed intake ( < 0.01), BW gain ( < 0.001), tibia ash ( < 0.01), and tibia breaking strength ( < 0.05). The influence of enzymes was more pronounced in sorghum-based diets than in wheat-based diets. Birds fed wheat-based diets had greater ileal digestibility of His, Met, Val, Phe, Ile, Leu, Trp, Glu, Pro, Ala, Tyr, and Cys compared with those fed sorghum-based diets ( < 0.05). Across grain types, NCP had greater apparent ileal digestibility of Met, Lys, Ser, Pro, Gly, and Cys than NC ( < 0.05). The results suggest that wheat is superior to sorghum for broilers, as expected, but that enzyme supplementation has the capability to restore the compromised bird performance due to feeding sorghum. In addition, compared with nonstarch polysaccharide-degrading enzymes and phytase (500 FTU/kg feed) added in concert, phytase supplemented at 1,000 FTU/kg resulted in a further improvement of some of the performance and bone mineralization parameters in male broilers fed sorghum-based diets.
[Mh] Termos MeSH primário: 6-Fitase/metabolismo
Ração Animal/análise
Galinhas/crescimento & desenvolvimento
Dieta/veterinária
Sorghum/química
Triticum/química
[Mh] Termos MeSH secundário: Aminoácidos/metabolismo
Fenômenos Fisiológicos da Nutrição Animal/efeitos dos fármacos
Animais
Calcificação Fisiológica/efeitos dos fármacos
Galinhas/microbiologia
Suplementos Nutricionais/análise
Digestão/efeitos dos fármacos
Grãos Comestíveis/metabolismo
Microbioma Gastrointestinal
Íleo/metabolismo
Masculino
Minerais
Sorghum/metabolismo
Triticum/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Amino Acids); 0 (Minerals); 68439-86-1 (bone ash); EC 3.1.3.26 (6-Phytase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.2527/jas.2016.0411



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