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[PMID]:28867285
[Au] Autor:Woznica A; Gerdt JP; Hulett RE; Clardy J; King N
[Ad] Endereço:Howard Hughes Medical Institute, and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
[Ti] Título:Mating in the Closest Living Relatives of Animals Is Induced by a Bacterial Chondroitinase.
[So] Source:Cell;170(6):1175-1183.e11, 2017 Sep 07.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We serendipitously discovered that the marine bacterium Vibrio fischeri induces sexual reproduction in one of the closest living relatives of animals, the choanoflagellate Salpingoeca rosetta. Although bacteria influence everything from nutrition and metabolism to cell biology and development in eukaryotes, bacterial regulation of eukaryotic mating was unexpected. Here, we show that a single V. fischeri protein, the previously uncharacterized EroS, fully recapitulates the aphrodisiac-like activity of live V. fischeri. EroS is a chondroitin lyase; although its substrate, chondroitin sulfate, was previously thought to be an animal synapomorphy, we demonstrate that S. rosetta produces chondroitin sulfate and thus extend the ancestry of this important glycosaminoglycan to the premetazoan era. Finally, we show that V. fischeri, purified EroS, and other bacterial chondroitin lyases induce S. rosetta mating at environmentally relevant concentrations, suggesting that bacteria likely regulate choanoflagellate mating in nature.
[Mh] Termos MeSH primário: Aliivibrio fischeri/enzimologia
Coanoflagelados/microbiologia
Coanoflagelados/fisiologia
Condroitinases e Condroitim Liases/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/química
Coanoflagelados/citologia
Sulfatos de Condroitina/metabolismo
Meiose
Reprodução
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 9007-28-7 (Chondroitin Sulfates); EC 4.2.2.- (Chondroitinases and Chondroitin Lyases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE


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[PMID]:27677277
[Au] Autor:Wang W; Wang J; Li F
[Ad] Endereço:National Glycoengineering Research Center, Shandong University, 27 S. Shanda Road, Jinan, 250100, China.
[Ti] Título:Hyaluronidase and Chondroitinase.
[So] Source:Adv Exp Med Biol;925:75-87, 2017.
[Is] ISSN:0065-2598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glycosaminoglycans (GAGs) are important constituents of the extracellular matrix that make significant contributions to biological processes and have been implicated in a wide variety of diseases. GAG-degrading enzymes with different activities have been found in various animals and microorganisms, and they play an irreplaceable role in the structure and function studies of GAGs. As two kind of important GAG-degrading enzymes, hyaluronidase (HAase) and chondroitinase (CSase) have been widely studied and increasing evidence has shown that, in most cases, their substrate specificities overlap and thus the "HAase" or "CSase" terms may be improper or even misnomers. Different from previous reviews, this article combines HAase and CSase together to discuss the traditional classification, substrate specificity, degradation pattern, new resources and naming of these enzymes.
[Mh] Termos MeSH primário: Condroitinases e Condroitim Liases/química
Células Eucarióticas/química
Matriz Extracelular/química
Glicosaminoglicanos/metabolismo
Hialuronoglucosaminidase/química
[Mh] Termos MeSH secundário: Animais
Bactérias/química
Bactérias/enzimologia
Configuração de Carboidratos
Sequência de Carboidratos
Condroitinases e Condroitim Liases/classificação
Condroitinases e Condroitim Liases/isolamento & purificação
Condroitinases e Condroitim Liases/metabolismo
Células Eucarióticas/citologia
Glicosaminoglicanos/química
Seres Humanos
Hialuronoglucosaminidase/classificação
Hialuronoglucosaminidase/isolamento & purificação
Hialuronoglucosaminidase/metabolismo
Hidrólise
Cinética
Especificidade por Substrato
Vírus/química
Vírus/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Glycosaminoglycans); EC 3.2.1.35 (Hyaluronoglucosaminidase); EC 4.2.2.- (Chondroitinases and Chondroitin Lyases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160929
[St] Status:MEDLINE
[do] DOI:10.1007/5584_2016_54


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[PMID]:27687161
[Au] Autor:Neira JL; Medina-Carmona E; Hernández-Cifre JG; Montoliu-Gaya L; Cámara-Artigás A; Seffouh I; Gonnet F; Daniel R; Villegas S; de la Torre JG; Pey AL; Li F
[Ad] Endereço:Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche, Alicante, Spain; Biocomputation and Complex Systems Physics Institute, Zaragoza, Spain. Electronic address: jlneira@umh.es.
[Ti] Título:The chondroitin sulfate/dermatan sulfate 4-O-endosulfatase from marine bacterium Vibrio sp FC509 is a dimeric species: Biophysical characterization of an endosulfatase.
[So] Source:Biochimie;131:85-95, 2016 Dec.
[Is] ISSN:1638-6183
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Sulfatases catalyze hydrolysis of sulfate groups. They have a key role in regulating the sulfation states that determine the function of several scaffold molecules. Currently, there are no studies of the conformational stability of endosulfatases. In this work, we describe the structural features and conformational stability of a 4-O-endosulfatase (EndoV) from a marine bacterium, which removes specifically the 4-O-sulfate from chondroitin sulfate/dermatan sulfate. For that purpose, we have used several biophysical techniques, namely, fluorescence, circular dichroism (CD), FTIR spectroscopy, analytical ultracentrifugation (AUC), differential scanning calorimetry (DSC), mass spectrometry (MS), dynamic light scattering (DLS) and size exclusion chromatography (SEC). The protein was a dimer with an elongated shape. EndoV acquired a native-like structure in a narrow pH range (7.0-9.0); it is within this range where the protein shows the maximum of enzymatic activity. The dimerization did not involve the presence of disulphide-bridges as suggested by AUC, SEC and DLS experiments in the presence of ß-mercaptoethanol (ß-ME). EndoV secondary structure is formed by a mixture of α and ß-sheet topology, as judged by deconvolution of CD and FTIR spectra. Thermal and chemical denaturations showed irreversibility and the former indicates that protein did not unfold completely during heating.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Sulfatos de Condroitina/metabolismo
Condroitinases e Condroitim Liases/metabolismo
Dermatan Sulfato/análogos & derivados
Vibrio/enzimologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Biocatálise
Fenômenos Biofísicos
Varredura Diferencial de Calorimetria
Condroitinases e Condroitim Liases/química
Cromatografia em Gel
Dicroísmo Circular
Dermatan Sulfato/metabolismo
Concentração de Íons de Hidrogênio
Desnaturação Proteica
Multimerização Proteica
Desdobramento de Proteína
Espectroscopia de Infravermelho com Transformada de Fourier
Sulfatos/metabolismo
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Sulfates); 0 (dermatan sulfate chondroitin sulfate); 24967-94-0 (Dermatan Sulfate); 9007-28-7 (Chondroitin Sulfates); EC 4.2.2.- (Chondroitinases and Chondroitin Lyases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161001
[St] Status:MEDLINE


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[PMID]:27349609
[Au] Autor:Xiong LL; Li Y; Shang FF; Chen SW; Chen H; Ju SM; Zou Y; Tian HL; Wang TH; Luo CZ; Wang XY
[Ad] Endereço:Department of Anesthesiology, Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
[Ti] Título:Chondroitinase administration and pcDNA3.1-BDNF-BMSC transplantation promote motor functional recovery associated with NGF expression in spinal cord-transected rat.
[So] Source:Spinal Cord;54(12):1088-1095, 2016 Dec.
[Is] ISSN:1476-5624
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:STUDY DESIGN: We evaluated whether combination of chondroitinase (chABC) administration and brain-derived neurotrophic factor (BDNF)-mesenchymal stem cell (MSC) transplantation could provide an optimal effect for the treatment of spinal cord injury (SCI) subjected to complete transection. OBJECTIVES: Behavioral assessments and DBA tracing were used to evaluate the effects of combination of chABC administration and BDNF-MSC transplantation on axonal regeneration and functional improvement in SCT rats. SETTING: Sichuan, ChinaMethods:Bone mesenchymal stem cells (BMSCs) were cultured and overexpressed BDNF recombinant vector was constructed into MSCs, then transplanted into the impaired spinal cord, together with chABC administration. Finally, the cortical spinal tract regeneration was detected by DBA tracing at 4 weeks post operation, and the expression of nerve growth factor (NGF), BDNF, neurotrophic factor (NT)-3, NT-4, fibroblast growth factor (FGF-2)-2, B cell lymphoma 2 (BCL-2) assaciated X protein (BAX) and BCL-2 in the caudal cord tissues was assessed by reverse transcription-PCR. RESULTS: Animals received both BDNF-BMSC transplantation and chABC administration presented the best functional recovery and obvious axonal regeneration. Moreover, NGF expression was significantly higher than that in the other groups. CONCLUSION: Co-treated strategy could effectively promote motor functional recovery and axonal regeneration in SCT rats associated with NGF upregulation.
[Mh] Termos MeSH primário: Fator Neurotrófico Derivado do Encéfalo/metabolismo
Condroitinases e Condroitim Liases/administração & dosagem
Transplante de Células-Tronco Mesenquimais/métodos
Fator de Crescimento Neural/metabolismo
Fármacos Neuroprotetores/administração & dosagem
Traumatismos da Medula Espinal/terapia
[Mh] Termos MeSH secundário: Animais
Transplante de Medula Óssea/métodos
Fator Neurotrófico Derivado do Encéfalo/genética
Células Cultivadas
Modelos Animais de Doenças
Feminino
Células Mesenquimais Estromais/metabolismo
Regeneração Nervosa/fisiologia
Técnicas de Rastreamento Neuroanatômico
Ratos
Recuperação de Função Fisiológica/fisiologia
Medula Espinal/metabolismo
Medula Espinal/patologia
Traumatismos da Medula Espinal/metabolismo
Traumatismos da Medula Espinal/patologia
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Brain-Derived Neurotrophic Factor); 0 (Neuroprotective Agents); 9061-61-4 (Nerve Growth Factor); EC 4.2.2.- (Chondroitinases and Chondroitin Lyases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160629
[St] Status:MEDLINE
[do] DOI:10.1038/sc.2016.55


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[PMID]:27016753
[Au] Autor:Namburi RB; Berteau O; Spillmann D; Rossi M
[Ad] Endereço:Department of Medical Biochemistry and Microbiology (IMBIM), Uppsala University, BMC, Box 582, SE-751 23 Uppsala, Sweden. Electronic address: Ramesh.B.Namburi@imbim.uu.se.
[Ti] Título:Chondroitinase AC: A host-associated genetic feature of Helicobacter bizzozeronii.
[So] Source:Vet Microbiol;186:21-7, 2016 Apr 15.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Investigating mechanisms involved in host adaptation is crucial to understand pathogen evolution. Helicobacter species appear to have a host species-specific tropism, coevolving with their natural hosts, and to develop several strategies allowing the colonization of the stomach throughout lifetime of their hosts. However, little is known about genetic features associated with the adaptation to a specific animal host. In this study we discovered a polysaccharide lyase that is expressed by the canine-associated species H. bizzozeronii and acts as chondroitinase AC-type lyase of broad specificity. Except for its low pH-optimum between pH 4.0 and pH 5.5, the properties of the H. bizzozeronii chondroitin lyase AC resemble the ones from Arthrobacter aurescens. However, homologues of this gene have been detected only in Helicobacter species colonizing the canine and feline gastric mucosa. Since a unique feature of the canine stomach is the secretion of chondroitin-4-sulphate in the gastric juice of the fundus mucosa by chief cells, the expression of chondroitinase AC by H. bizzozeronii is likely the consequence of adaptation of this bacterium to its host and a potential link to gastric disorders in dogs.
[Mh] Termos MeSH primário: Condroitinases e Condroitim Liases/química
Doenças do Cão/microbiologia
Infecções por Helicobacter/microbiologia
Helicobacter/enzimologia
Helicobacter/genética
Interações Hospedeiro-Parasita/genética
Modelos Moleculares
[Mh] Termos MeSH secundário: Adaptação Fisiológica/genética
Animais
Gatos
Sulfatos de Condroitina/química
Sulfatos de Condroitina/metabolismo
Condroitinases e Condroitim Liases/genética
Condroitinases e Condroitim Liases/metabolismo
Dissacarídeos/metabolismo
Doenças do Cão/enzimologia
Cães
Mucosa Gástrica/metabolismo
Concentração de Íons de Hidrogênio
Estrutura Terciária de Proteína
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Disaccharides); 9007-28-7 (Chondroitin Sulfates); EC 4.2.2.- (Chondroitinases and Chondroitin Lyases)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160328
[St] Status:MEDLINE


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[PMID]:26852702
[Au] Autor:Jin Y; Bouyer J; Shumsky JS; Haas C; Fischer I
[Ad] Endereço:Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia PA 19129, United States. Electronic address: yjin@drexelmed.edu.
[Ti] Título:Transplantation of neural progenitor cells in chronic spinal cord injury.
[So] Source:Neuroscience;320:69-82, 2016 Apr 21.
[Is] ISSN:1873-7544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previous studies demonstrated that neural progenitor cells (NPCs) transplanted into a subacute contusion injury improve motor, sensory, and bladder function. In this study we tested whether transplanted NPCs can also improve functional recovery after chronic spinal cord injury (SCI) alone or in combination with the reduction of glial scar and neurotrophic support. Adult rats received a T10 moderate contusion. Thirteen weeks after the injury they were divided into four groups and received either: 1. Medium (control), 2. NPC transplants, 3. NPC+lentivirus vector expressing chondroitinase, or 4. NPC+lentivirus vectors expressing chondroitinase and neurotrophic factors. During the 8 weeks post-transplantation the animals were tested for functional recovery and eventually analyzed by anatomical and immunohistochemical assays. The behavioral tests for motor and sensory function were performed before and after injury, and weekly after transplantation, with some animals also tested for bladder function at the end of the experiment. Transplant survival in the chronic injury model was variable and showed NPCs at the injury site in 60% of the animals in all transplantation groups. The NPC transplants comprised less than 40% of the injury site, without significant anatomical or histological differences among the groups. All groups also showed similar patterns of functional deficits and recovery in the 12 weeks after injury and in the 8 weeks after transplantation using the Basso, Beattie, and Bresnahan rating score, the grid test, and the Von Frey test for mechanical allodynia. A notable exception was group 4 (NPC together with chondroitinase and neurotrophins), which showed a significant improvement in bladder function. This study underscores the therapeutic challenges facing transplantation strategies in a chronic SCI in which even the inclusion of treatments designed to reduce scarring and increase neurotrophic support produce only modest functional improvements. Further studies will have to identify the combination of acute and chronic interventions that will augment the survival and efficacy of neural cell transplants.
[Mh] Termos MeSH primário: Células-Tronco Neurais/transplante
Recuperação de Função Fisiológica
Traumatismos da Medula Espinal
Transplante de Células-Tronco/métodos
[Mh] Termos MeSH secundário: Animais
Condroitinases e Condroitim Liases/farmacologia
Doença Crônica
Modelos Animais de Doenças
Feminino
Imuno-Histoquímica
Fatores de Crescimento Neural/farmacologia
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Nerve Growth Factors); EC 4.2.2.- (Chondroitinases and Chondroitin Lyases)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160209
[St] Status:MEDLINE


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[PMID]:26828927
[Au] Autor:Kamma-Lorger CS; Pinali C; Martínez JC; Harris J; Young RD; Bredrup C; Crosas E; Malfois M; Rødahl E; Meek KM; Knupp C
[Ad] Endereço:NCD-BL11, ALBA Synchrotron Light Source, Cerdanyola del Vallés, 08290, Barcelona, Spain.
[Ti] Título:Role of Decorin Core Protein in Collagen Organisation in Congenital Stromal Corneal Dystrophy (CSCD).
[So] Source:PLoS One;11(2):e0147948, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The role of Decorin in organising the extracellular matrix was examined in normal human corneas and in corneas from patients with Congenital Stromal Corneal Dystrophy (CSCD). In CSCD, corneal clouding occurs due to a truncating mutation (c.967delT) in the decorin (DCN) gene. Normal human Decorin protein and the truncated one were reconstructed in silico using homology modelling techniques to explore structural changes in the diseased protein. Corneal CSCD specimens were also examined using 3-D electron tomography and Small Angle X-ray diffraction (SAXS), to image the collagen-proteoglycan arrangement and to quantify fibrillar diameters, respectively. Homology modelling showed that truncated Decorin had a different spatial geometry to the normal one, with the truncation removing a major part of the site that interacts with collagen, compromising its ability to bind effectively. Electron tomography showed regions of abnormal stroma, where collagen fibrils came together to form thicker fibrillar structures, showing that Decorin plays a key role in the maintenance of the order in the normal corneal extracellular matrix. Average diameter of individual fibrils throughout the thickness of the cornea however remained normal.
[Mh] Termos MeSH primário: Colágeno/metabolismo
Distrofias Hereditárias da Córnea/metabolismo
Decorina/metabolismo
[Mh] Termos MeSH secundário: Condroitinases e Condroitim Liases/metabolismo
Córnea/patologia
Distrofias Hereditárias da Córnea/patologia
Decorina/química
Seres Humanos
Imagem Tridimensional
Modelos Moleculares
Proteínas Mutantes/química
Proteínas Mutantes/metabolismo
Multimerização Proteica
Espalhamento a Baixo Ângulo
Homologia Estrutural de Proteína
Tomografia
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DCN protein, human); 0 (Decorin); 0 (Mutant Proteins); 9007-34-5 (Collagen); EC 4.2.2.- (Chondroitinases and Chondroitin Lyases)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160202
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0147948


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[PMID]:26662555
[Au] Autor:Nissi MJ; Salo EN; Tiitu V; Liimatainen T; Michaeli S; Mangia S; Ellermann J; Nieminen MT
[Ad] Endereço:Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
[Ti] Título:Multi-parametric MRI characterization of enzymatically degraded articular cartilage.
[So] Source:J Orthop Res;34(7):1111-20, 2016 Jul.
[Is] ISSN:1554-527X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Several laboratory and rotating frame quantitative MRI parameters were evaluated and compared for detection of changes in articular cartilage following selective enzymatic digestion. Bovine osteochondral specimens were subjected to 44 h incubation in control medium or in collagenase or chondroitinase ABC to induce superficial collagen or proteoglycan (glycosaminoglycan) alterations. The samples were scanned at 9.4 T for T1 , T1 Gd (dGEMRIC), T2 , adiabatic T1 ρ , adiabatic T2 ρ , continuous-wave T1 ρ , TRAFF2 , and T1 sat relaxation times and for magnetization transfer ratio (MTR). For reference, glycosaminoglycan content, collagen fibril orientation and biomechanical properties were determined. Changes primarily in the superficial cartilage were noted after enzymatic degradation. Most of the studied parameters were sensitive to the destruction of collagen network, whereas glycosaminoglycan depletion was detected only by native T1 and T1 Gd relaxation time constants throughout the tissue and by MTR superficially. T1 , adiabatic T1 ρ , adiabatic T2 ρ , continuous-wave T1 ρ , and T1 sat correlated significantly with the biomechanical properties while T1 Gd correlated with glycosaminoglycan staining. The findings indicated that most of the studied MRI parameters were sensitive to both glycosaminoglycan content and collagen network integrity, with changes due to enzymatic treatment detected primarily in the superficial tissue. Strong correlation of T1 , adiabatic T1ρ , adiabatic T2 ρ , continuous-wave T1 ρ , and T1 sat with the altered biomechanical properties, reflects that these parameters were sensitive to critical functional properties of cartilage. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1111-1120, 2016.
[Mh] Termos MeSH primário: Cartilagem Articular/diagnóstico por imagem
Imagem por Ressonância Magnética/métodos
[Mh] Termos MeSH secundário: Animais
Bovinos
Condroitinases e Condroitim Liases
Colagenases
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.4.24.- (Collagenases); EC 4.2.2.- (Chondroitinases and Chondroitin Lyases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE
[do] DOI:10.1002/jor.23127


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[PMID]:26572480
[Au] Autor:Wende FJ; Gohil S; Mojarradi H; Gerfaud T; Nord LI; Karlsson A; Boiteau JG; Kenne AH; Sandström C
[Ad] Endereço:Department of Chemistry and Biotechnology, Uppsala BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-750 07 Uppsala, Sweden. Electronic address: Frida.Wende@slu.se.
[Ti] Título:Determination of substitution positions in hyaluronic acid hydrogels using NMR and MS based methods.
[So] Source:Carbohydr Polym;136:1348-57, 2016 Jan 20.
[Is] ISSN:1879-1344
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In hydrogels of cross-linked polysaccharides, the total amount of cross-linker and the degree of cross-linking influence the properties of the hydrogel. The substitution position of the cross-linker on the polysaccharide is another parameter that can influence hydrogel properties; hence methods for detailed structural analysis of the substitution pattern are required. NMR and LC-MS methods were developed to determine the positions and amounts of substitution of 1,4-butanediol diglycidyl ether (BDDE) on hyaluronic acid (HA), and for the first time it is shown that BDDE can react with any of the four available hydroxyl groups of the HA disaccharide repeating unit. This was achieved by studying di-, tetra-, and hexasaccharides obtained from degradation of BDDE cross-linked HA hydrogel by chondroitinase. Furthermore, amount of linker substitution at each position was shown to be dependent on the size of the oligosaccharides. For the disaccharide, substitutions were predominantly at ΔGlcA-OH2 and GlcNAc-OH6 while in the tetra- and hexasaccharides, it was mainly at the reducing end GlcNAc-OH4. In the disaccharide there was no substitution at this position. Since chondroitinase is able to completely hydrolyse non-substituted HA into unsaturated disaccharides, these results indicate that the enzyme is prevented to cleave on the non-reducing side of an oligosaccharide substituted at the reducing end GlcNAc-OH4. The procedure can be adopted for the determination of substitution positions in other types of polymers.
[Mh] Termos MeSH primário: Ácido Hialurônico/química
Hidrogéis/química
[Mh] Termos MeSH secundário: Butileno Glicóis/química
Condroitinases e Condroitim Liases/metabolismo
Hidrólise
Espectroscopia de Ressonância Magnética
Espectrometria de Massas
Oligossacarídeos/química
Proteus vulgaris/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Butylene Glycols); 0 (Hydrogels); 0 (Oligosaccharides); 9004-61-9 (Hyaluronic Acid); E9MU425FIB (1,4-bis(2,3-epoxypropoxy)butane); EC 4.2.2.- (Chondroitinases and Chondroitin Lyases)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151117
[Lr] Data última revisão:
151117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151118
[St] Status:MEDLINE


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[PMID]:25319196
[Au] Autor:Kasinathan N; Volety SM; Josyula VR
[Ad] Endereço:a Department of Pharmaceutical Biotechnology , Manipal College of Pharmaceutical Sciences, Manipal College of Pharmaceutical Sciences, Manipal University , Manipal , Karnataka , India.
[Ti] Título:Chondroitinase: A promising therapeutic enzyme.
[So] Source:Crit Rev Microbiol;42(3):474-84, 2016 May.
[Is] ISSN:1549-7828
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Even after 20 years of granting orphan status for chondroitinase by US FDA, there is no visible outcome in terms of clinical use. The reasons are many. One of them could be lack of awareness regarding the biological application of the enzyme. The biological activity of chondroitinase is due to its ability to act on chondroitin sulfate proteoglycans (CSPGs). CSPGs are needed for normal functioning of the body. An increase or decrease in the level of CSPGs results in various pathological conditions. Chondroitinase is useful in conditions where there is an increase in the level of CSPGs, namely spinal cord injury, vitreous attachment and cancer. Over the last decade, various animal studies showed that chondroitinase could be a good drug candidate. Research focusing on developing a suitable carrier system for delivering chondroitinase needs to be carried out so that pharmacological activity observed in vitro and preclinical studies could be translated to clinical use. Further studies on distribution of chondroitinase as well need to be focused so that chondroitinase with desired attributes could be discovered. The present review article discusses about various biological applications of chondroitinase, drug delivery systems to deliver the enzyme and distribution of chondroitinase among microbes.
[Mh] Termos MeSH primário: Condroitinases e Condroitim Liases/farmacologia
[Mh] Termos MeSH secundário: Animais
Proteoglicanas de Sulfatos de Condroitina/metabolismo
Condroitinases e Condroitim Liases/metabolismo
Seres Humanos
Traumatismos da Medula Espinal/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Chondroitin Sulfate Proteoglycans); EC 4.2.2.- (Chondroitinases and Chondroitin Lyases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170118
[Lr] Data última revisão:
170118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141017
[St] Status:MEDLINE
[do] DOI:10.3109/1040841X.2014.959893



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