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  1 / 1768 MEDLINE  
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[PMID]:28822946
[Au] Autor:Xu ZR; Cai SW; Huang WX; Liu RX; Xiong ZT
[Ad] Endereço:School of Resource and Environmental Sciences, Wuhan University, Wuhan, Hubei, People's Republic of China.
[Ti] Título:Differential expression of vacuolar and defective cell wall invertase genes in roots and seeds of metalliferous and non-metalliferous populations of Rumex dentatus under copper stress.
[So] Source:Ecotoxicol Environ Saf;147:17-25, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Acid invertase activities in roots and young seeds of a metalliferous population (MP) of Rumex dentatus were previously observed to be significantly higher than those of a non-metalliferous population (NMP) under Cu stress. To date, no acid invertase gene has been cloned from R. dentatus. Here, we isolated four full-length cDNAs from the two populations of R. dentatus, presumably encoding cell wall (RdnCIN1 and RdmCIN1 from the NMP and MP, respectively) and vacuolar invertases (RdnVIN1 and RdmVIN1 from the NMP and MP, respectively). Unexpectedly, RdnCIN1 and RdmCIN1 most likely encode special defective invertases with highly attenuated sucrose-hydrolyzing capacity. The transcript levels of RdmCIN1 were significantly higher than those of RdnCIN1 in roots and young seeds under Cu stress, whereas under control conditions, the former was initially lower than the latter. Unexpected high correlations were observed between the transcript levels of RdnCIN1 and RdmCIN1 and the activity of cell wall invertase, even though RdnCIN1 and RdmCIN1 do not encode catalytically active invertases. Similarly, the transcript levels of RdmVIN1 in roots and young seeds were increased under Cu stress, whereas those of RdnVIN1 were decreased. The high correlations between the transcript levels of RdnVIN1 and RdmVIN1 and the activity of vacuolar invertase indicate that RdnVIN1 and RdmVIN1 might control distinct vacuolar invertase activities in the two populations. Moreover, a possible indirect role for acid invertases in Cu tolerance, mediated by generating a range of sugars used as nutrients and signaling molecules, is discussed.
[Mh] Termos MeSH primário: Parede Celular/efeitos dos fármacos
Cobre/toxicidade
Rumex/efeitos dos fármacos
Poluentes do Solo/toxicidade
Vacúolos/efeitos dos fármacos
beta-Frutofuranosidase/genética
[Mh] Termos MeSH secundário: Parede Celular/enzimologia
Parede Celular/genética
Cobre/metabolismo
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Genes de Plantas
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/enzimologia
Raízes de Plantas/metabolismo
Rumex/genética
Rumex/metabolismo
Sementes/efeitos dos fármacos
Sementes/enzimologia
Sementes/genética
Poluentes do Solo/metabolismo
Vacúolos/enzimologia
Vacúolos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil Pollutants); 789U1901C5 (Copper); EC 3.2.1.26 (beta-Fructofuranosidase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170821
[St] Status:MEDLINE


  2 / 1768 MEDLINE  
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[PMID]:28597466
[Au] Autor:Wiberley-Bradford AE; Bethke PC
[Ad] Endereço:Department of Horticulture, University of Wisconsin-Madison, Madison, WI, USA.
[Ti] Título:Suppression of the vacuolar invertase gene delays senescent sweetening in chipping potatoes.
[So] Source:J Sci Food Agric;98(1):354-360, 2018 Jan.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Potato chip processors require potato tubers that meet quality specifications for fried chip color, and color depends largely upon tuber sugar contents. At later times in storage, potatoes accumulate sucrose, glucose, and fructose. This developmental process, senescent sweetening, manifests as a blush of color near the center of the fried chip, becomes more severe with time, and limits the storage period. Vacuolar invertase (VInv) converts sucrose to glucose and fructose and is hypothesized to play a role in senescent sweetening. To test this hypothesis, senescent sweetening was quantified in multiple lines of potato with reduced VInv expression. RESULTS: Chip darkening from senescent sweetening was delayed by about 4 weeks for tubers with reduced VInv expression. A strong positive correlation between frequency of dark chips and tuber hexose content was observed. Tubers with reduced VInv expression had lower hexose to sucrose ratios than controls. CONCLUSION: VInv activity contributes to reducing sugar accumulation during senescent sweetening. Sucrose breakdown during frying may contribute to chip darkening. Suppressing VInv expression increases the storage period of the chipping potato crop, which is an important consideration, as potatoes with reduced VInv expression are entering commercial production in the USA. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Aromatizantes/metabolismo
Proteínas de Plantas/genética
Solanum tuberosum/enzimologia
beta-Frutofuranosidase/genética
[Mh] Termos MeSH secundário: Culinária
Aromatizantes/química
Seres Humanos
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Tubérculos/química
Tubérculos/enzimologia
Tubérculos/genética
Solanum tuberosum/química
Solanum tuberosum/genética
Paladar
beta-Frutofuranosidase/química
beta-Frutofuranosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavoring Agents); 0 (Plant Proteins); EC 3.2.1.26 (beta-Fructofuranosidase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8478


  3 / 1768 MEDLINE  
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[PMID]:27777307
[Au] Autor:Xie J; Cai K; Hu HX; Jiang YL; Yang F; Hu PF; Cao DD; Li WF; Chen Y; Zhou CZ
[Ad] Endereço:From the Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei Anhui 230027, China.
[Ti] Título:Structural Analysis of the Catalytic Mechanism and Substrate Specificity of Anabaena Alkaline Invertase InvA Reveals a Novel Glucosidase.
[So] Source:J Biol Chem;291(49):25667-25677, 2016 Dec 02.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Invertases catalyze the hydrolysis of sucrose to glucose and fructose, thereby playing a key role in primary metabolism and plant development. According to the optimum pH, invertases are classified into acid invertases (Ac-Invs) and alkaline/neutral invertases (A/N-Invs), which share no sequence homology. Compared with Ac-Invs that have been extensively studied, the structure and catalytic mechanism of A/N-Invs remain unknown. Here we report the crystal structures of Anabaena alkaline invertase InvA, which was proposed to be the ancestor of modern plant A/N-Invs. These structures are the first in the GH100 family. InvA exists as a hexamer in both crystal and solution. Each subunit consists of an (α/α) barrel core structure in addition to an insertion of three helices. A couple of structures in complex with the substrate or products enabled us to assign the subsites -1 and +1 specifically binding glucose and fructose, respectively. Structural comparison combined with enzymatic assays indicated that Asp-188 and Glu-414 are putative catalytic residues. Further analysis of the substrate binding pocket demonstrated that InvA possesses a stringent substrate specificity toward the α1,2-glycosidic bond of sucrose. Together, we suggest that InvA and homologs represent a novel family of glucosidases.
[Mh] Termos MeSH primário: Anabaena/enzimologia
Proteínas de Bactérias/química
beta-Frutofuranosidase/química
[Mh] Termos MeSH secundário: Anabaena/genética
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Cristalografia por Raios X
Frutose/química
Frutose/metabolismo
Glucose/química
Glucose/metabolismo
Domínios Proteicos
Sacarose/química
Sacarose/metabolismo
beta-Frutofuranosidase/genética
beta-Frutofuranosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 30237-26-4 (Fructose); 57-50-1 (Sucrose); EC 3.2.1.26 (beta-Fructofuranosidase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171202
[Lr] Data última revisão:
171202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


  4 / 1768 MEDLINE  
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[PMID]:28945799
[Au] Autor:Battaglia ME; Martin MV; Lechner L; Martínez-Noël GMA; Salerno GL
[Ad] Endereço:Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC-CONICET) and Fundación para Investigaciones Biológicas Aplicadas (FIBA), Mar del Plata, Argentina.
[Ti] Título:The riddle of mitochondrial alkaline/neutral invertases: A novel Arabidopsis isoform mainly present in reproductive tissues and involved in root ROS production.
[So] Source:PLoS One;12(9):e0185286, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Alkaline/neutral invertases (A/N-Inv), glucosidases that irreversibly hydrolyze sucrose into glucose and fructose, play significant roles in plant growth, development, and stress adaptation. They occur as multiple isoforms located in the cytosol or organelles. In Arabidopsis thaliana, two mitochondrial A/N-Inv genes (A/N-InvA and A/N-InvC) have already been investigated. In this study, we functionally characterized A/N-InvH, a third Arabidopsis gene coding for a mitochondrial-targeted protein. The phenotypic analysis of knockout mutant plants (invh) showed a severely reduced shoot growth, while root development was not affected. The emergence of the first floral bud and the opening of the first flower were the most affected stages, presenting a significant delay. A/N-InvH transcription is markedly active in reproductive tissues. It is also expressed in the elongation and apical meristem root zones. Our results show that A/N-InvH expression is not evident in photosynthetic tissues, despite being of relevance in developmental processes and mitochondrial functional status. NaCl and mannitol treatments increased A/N-InvH expression twofold in the columella root cap. Moreover, the absence of A/N-InvH prevented ROS formation, not only in invh roots of salt- and ABA-treated seedlings but also in invh control roots. We hypothesize that this isoform may take part in the ROS/sugar (sucrose or its hydrolysis products) signaling pathway network, involved in reproductive tissue development, cell elongation, and abiotic stress responses.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/enzimologia
beta-Frutofuranosidase/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/química
Proteínas de Arabidopsis/genética
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Técnicas de Inativação de Genes
Genes de Plantas
Concentração de Íons de Hidrogênio
Isoenzimas/química
Isoenzimas/genética
Isoenzimas/metabolismo
Mitocôndrias/enzimologia
Proteínas Mitocondriais/química
Proteínas Mitocondriais/genética
Proteínas Mitocondriais/metabolismo
Fenótipo
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Plantas Geneticamente Modificadas
Regiões Promotoras Genéticas
Espécies Reativas de Oxigênio/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Distribuição Tecidual
beta-Frutofuranosidase/química
beta-Frutofuranosidase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Isoenzymes); 0 (Mitochondrial Proteins); 0 (Reactive Oxygen Species); 0 (Recombinant Proteins); EC 3.2.1.26 (beta-Fructofuranosidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185286


  5 / 1768 MEDLINE  
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[PMID]:28715279
[Au] Autor:Nagaya M; Kimura M; Gozu Y; Sato S; Hirano K; Tochio T; Nishikawa A; Tonozuka T
[Ad] Endereço:a Department of Applied Biological Science , Tokyo University of Agriculture and Technology , Fuchu , Japan.
[Ti] Título:Crystal structure of a ß-fructofuranosidase with high transfructosylation activity from Aspergillus kawachii.
[So] Source:Biosci Biotechnol Biochem;81(9):1786-1795, 2017 Sep.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:ß-Fructofuranosidases belonging to glycoside hydrolase family (GH) 32 are enzymes that hydrolyze sucrose. Some GH32 enzymes also catalyze transfructosylation to produce fructooligosaccharides. We found that Aspergillus kawachii IFO 4308 ß-fructofuranosidase (AkFFase) produces fructooligosaccharides, mainly 1-kestose, from sucrose. We determined the crystal structure of AkFFase. AkFFase is composed of an N-terminal small component, a ß-propeller catalytic domain, an α-helical linker, and a C-terminal ß-sandwich, similar to other GH32 enzymes. AkFFase forms a dimer, and the dimerization pattern is different from those of other oligomeric GH32 enzymes. The complex structure of AkFFase with fructose unexpectedly showed that fructose binds both subsites -1 and +1, despite the fact that the catalytic residues were not mutated. Fructose at subsite +1 interacts with Ile146 and Glu296 of AkFFase via direct hydrogen bonds.
[Mh] Termos MeSH primário: Aspergillus/enzimologia
Frutose/metabolismo
beta-Frutofuranosidase/química
beta-Frutofuranosidase/metabolismo
[Mh] Termos MeSH secundário: Domínio Catalítico
Cristalografia por Raios X
Glicosilação
Modelos Moleculares
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
30237-26-4 (Fructose); EC 3.2.1.26 (beta-Fructofuranosidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1353405


  6 / 1768 MEDLINE  
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[PMID]:28658609
[Au] Autor:Vallarino JG; Yeats TH; Maximova E; Rose JK; Fernie AR; Osorio S
[Ad] Endereço:Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", University of Malaga- Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Department of Molecular Biology and Biochemistry, Campus de Teatinos, 29071, Málaga, Spain.
[Ti] Título:Postharvest changes in LIN5-down-regulated plants suggest a role for sugar deficiency in cuticle metabolism during ripening.
[So] Source:Phytochemistry;142:11-20, 2017 Oct.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The cell wall invertase gene (LIN5) was reported to be a key enzyme influencing sugar uptake of tomato (Solanum lycopersicum) fruit. It was additionally revealed to be a key regulator of total soluble solids content in fruit as well as for reproductive development, being mainly involved in flower development, early fruit and seed development but also in ripening. Here, we demonstrate that silencing of the LIN5 gene promotes changes affecting fruit cuticle development which has a direct effect on postharvest properties. Transformants were characterized by reduced transpirational water loss in mature fruits accompanied by several other changes in the cuticle. Quantitative chemical composition, coupled with microscopy of isolated cuticle fruits revealed that the cuticle of the transformants were characterized by an increase of the thickness as well as significant increase in the content of cuticle components (cutin, phenolic compounds, and waxes). Furthermore, detailed analysis of the waxes revealed that the transformants displayed changes in waxes composition, showing higher levels of n-alkanes and triterpenoids which can shift the proportion of crystalline and amorphous waxes and change the water flux through the cuticle. Expression of the genes involved in cuticle biosynthesis indicated that LIN5 influences the biosynthesis of components of the cuticle, indicating that this process is coupled to sugar uploading via a mechanism which links carbon supply with the capacity for fruit expansion.
[Mh] Termos MeSH primário: Carboidratos/análise
Epiderme Vegetal/metabolismo
Proteínas de Plantas/metabolismo
beta-Frutofuranosidase/metabolismo
[Mh] Termos MeSH secundário: Parede Celular/metabolismo
Regulação para Baixo
Frutas/enzimologia
Frutas/genética
Frutas/metabolismo
Regulação da Expressão Gênica de Plantas
Lycopersicon esculentum/enzimologia
Lycopersicon esculentum/genética
Lycopersicon esculentum/metabolismo
Lipídeos de Membrana/metabolismo
Fenóis/metabolismo
Ceras/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Membrane Lipids); 0 (Phenols); 0 (Plant Proteins); 0 (Waxes); 54990-88-4 (cutin); EC 3.2.1.26 (beta-Fructofuranosidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE


  7 / 1768 MEDLINE  
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[PMID]:28247243
[Au] Autor:Wang XQ; Zheng LL; Lin H; Yu F; Sun LH; Li LM
[Ad] Endereço:College of Food Science and Nutritional Engineering, China Agricultural University, Qinghua East Road No 17, Haidian District, Beijing, 100083, China. wangxqbj@163.com.
[Ti] Título:Grape hexokinases are involved in the expression regulation of sucrose synthase- and cell wall invertase-encoding genes by glucose and ABA.
[So] Source:Plant Mol Biol;94(1-2):61-78, 2017 May.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Hexokinase (HXK, EC 2.7.1.1) is a multifunctional protein that both is involved in catalyzing the first step of glycolysis and plays an important role in sugar signaling. However, the supporting genetic evidence on hexokinases (CsHXKs) from grape (Vitis vinifera L. cv. Cabernet Sauvignon) berries has been lacking. Here, to investigate the role of CsHXK isoforms as glucose (Glc) and abscisic acid (ABA) sensors, we cloned two hexokinase isozymes, CsHXK1 and CsHXK2 with highly conserved genomic structure of nine exons and eight introns. We also found adenosine phosphate binding, substrate recognition and connection sites in their putative proteins. During grape berry development, the expression profiles of two CsHXK isoforms, sucrose synthases (SuSys) and cell wall invertase (CWINV) genes increased concomitantly with high levels of endogenous Glc and ABA. Furthermore, we showed that in wild type grape berry calli (WT), glucose repressed the expression levels of sucrose synthase (SuSy) and cell wall invertase (CWINV) genes, while ABA increased their expression levels. ABA could not only effectively improve the expression levels of SuSy and CWINV, but also block the repression induced by glucose on the expression of both genes. However, after silencing CsHXK1 or CsHXK2 in grape calli, SuSy and CWINV expression were enhanced, and the expressions of the two genes are insensitive in response to Glc treatment. Interestingly, exogenous ABA alone could not or less increase SuSy and CWINV expression in silencing CsHXK1 or CsHXK2 grape calli compared to WT. Meantime, ABA could not block the repression induced by glucose on the expression of SuSy and CWINV in CsHXK1 or CsHXK2 mutants. Therefore, Glc signal transduction depends on the regulation of CsHXK1 or CsHXK2. ABA signal was also disturbed by CsHXK1 or CsHXK2 silencing. The present results provide new insights into the regulatory role of Glc and ABA on the enzymes related to sugar metabolism in grape berry.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Glucosiltransferases/metabolismo
Hexoquinase/metabolismo
Proteínas de Plantas/metabolismo
Vitis/enzimologia
beta-Frutofuranosidase/metabolismo
[Mh] Termos MeSH secundário: Ácido Abscísico/farmacologia
Sequência de Aminoácidos
Parede Celular/enzimologia
Frutas/enzimologia
Frutas/metabolismo
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Glucose/farmacologia
Glucosiltransferases/genética
Hexoquinase/genética
Modelos Moleculares
Filogenia
Proteínas de Plantas/genética
Conformação Proteica
beta-Frutofuranosidase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 72S9A8J5GW (Abscisic Acid); EC 2.4.1.- (Glucosyltransferases); EC 2.4.1.13 (sucrose synthase); EC 2.7.1.1 (Hexokinase); EC 3.2.1.26 (beta-Fructofuranosidase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-017-0593-9


  8 / 1768 MEDLINE  
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[PMID]:28203077
[Au] Autor:Zhu X; Sarwar M; Yue Q; Chen C; Li CZ
[Ad] Endereço:Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, Miami, FL, USA.
[Ti] Título:Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection.
[So] Source:Int J Nanomedicine;12:979-987, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL with a dynamic range of 1-200 ng mL . Considering the inherent advantages of the personal glucose meter, the demonstration of this device, therefore, should provide new opportunities for the monitoring of a wide range of biomarkers and various target analytes in connection with different molecular recognition events.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Automonitorização da Glicemia/instrumentação
Dano ao DNA
Desoxiguanosina/análogos & derivados
[Mh] Termos MeSH secundário: Biomarcadores/análise
Técnicas Biossensoriais/instrumentação
Desoxiguanosina/análise
Desenho de Equipamento
Glucose/metabolismo
Guanosina/análogos & derivados
Guanosina/química
Oxirredução
Smartphone
beta-Frutofuranosidase/química
beta-Frutofuranosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 12133JR80S (Guanosine); 3868-31-3 (8-hydroxyguanosine); 88847-89-6 (8-oxo-7-hydrodeoxyguanosine); EC 3.2.1.26 (beta-Fructofuranosidase); G9481N71RO (Deoxyguanosine); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S125437


  9 / 1768 MEDLINE  
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[PMID]:28193587
[Au] Autor:Huang D; Liu L; Zeng G; Xu P; Huang C; Deng L; Wang R; Wan J
[Ad] Endereço:College of Environmental Science and Engineering, Hunan University, Changsha 410082, People's Republic of China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, People's Republic of China. Electronic address: huangdanlian@hnu.e
[Ti] Título:The effects of rice straw biochar on indigenous microbial community and enzymes activity in heavy metal-contaminated sediment.
[So] Source:Chemosphere;174:545-553, 2017 May.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Owning to the potential in carbon sequestration and other environmental benefits, biochar has been widely used for in-situ environmental remediation. Understanding the biological effects of biochar is essential. The goal of this study was to explore the response of indigenous microbes under the stress of different concentrations of biochar. The results showed that biochar could significantly change physicochemical properties, enzymes activity and microbial community composition depending on biochar concentration and incubation time. When the concentration of biochar was 50 mg kg , the activities of invertase and alkaline phosphatase were obviously inhibited. Meanwhile, bacterial 16S rRNA and fungal 18S rRNA coding gene copies were decreased by 74% and 25%, respectively after 90 days of incubation. Additionally, the bacterial community succession occurred and the relative intensity of dominant species decreased when treated with high concentration of biochar. However, the activity of urease and alkaline phosphatase, as well as bacterial and fungal abundance, were increased when sediment was treated with 10 mg kg biochar. Relationships among physicochemical properties, heavy metals and microbes were analyzed by correlation analysis and redundancy analysis (RDA). Correlations between invertase activity and pH value in the experiment were significantly negative. Redundancy analysis showed physicochemical properties and heavy metals explained 92% of the variation in the bacterial DGGE profiles and organic matter content explained the majority (45%) of the variation. This study indicated that indigenous microbes could be affected by biochar either directly or indirectly via changing the physicochemical properties and heavy metals of sediment.
[Mh] Termos MeSH primário: Carvão Vegetal/toxicidade
Oryza
Microbiologia da Água
[Mh] Termos MeSH secundário: Fosfatase Alcalina/metabolismo
Bactérias/efeitos dos fármacos
Bactérias/enzimologia
Bactérias/genética
Proteínas de Bactérias/metabolismo
Proteínas Fúngicas/metabolismo
Fungos/efeitos dos fármacos
Fungos/enzimologia
Fungos/genética
Metais Pesados
RNA Bacteriano/genética
RNA Fúngico/genética
RNA Ribossômico 16S/genética
RNA Ribossômico 18S/genética
Solo/química
Urease/metabolismo
Poluentes Químicos da Água
beta-Frutofuranosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Fungal Proteins); 0 (Metals, Heavy); 0 (RNA, Bacterial); 0 (RNA, Fungal); 0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 18S); 0 (Soil); 0 (Water Pollutants, Chemical); 0 (biochar); 16291-96-6 (Charcoal); EC 3.1.3.1 (Alkaline Phosphatase); EC 3.2.1.26 (beta-Fructofuranosidase); EC 3.5.1.5 (Urease)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE


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[PMID]:28159248
[Au] Autor:Kurakake M; Hirotsu S; Shibata M; Takenaka Y; Kamioka T; Sakamoto T
[Ad] Endereço:Department of Marine Biotechnology, Fukuyama University, 1-985 Sanzo, Higashimura-cho, Fukuyama, Hiroshima 729-0292, Japan; Department of Life and Nutritional Science, Fukuyama University, 1-985 Sanzo, Higashimura-cho, Fukuyama, Hiroshima 729-0292, Japan. Electronic address: kurakake@fubac.fukuyama-
[Ti] Título:Effects of nonionic surfactants on pellet formation and the production of ß-fructofuranosidases from Aspergillus oryzae KB.
[So] Source:Food Chem;224:139-143, 2017 Jun 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aspergillus oryzae KB produces two ß-fructofuranosidases (F1 and F2). F1 has high transferring activity and produces fructooligosaccharides from sucrose. Mycelial growth pellets were altered by the addition of Tween 20, 40 and 80 (HLB=16.7, 15.6 and 15.0, respectively) in liquid medium cultures to form small spherical pellets. The particle size of the pellets decreased with the HLB value, which corresponds to an increase in surfactant hydrophobicity. Selective F1 production and pellet size were maximized using Tween 20. Adding polyoxyethylene oleyl ethers (POEs) with various degrees of polymerization (2, 7, 10, 20 and 50: HLB=7.7, 10.7, 14.7, 17.2 and 18.2, respectively) was investigated. A minimum mean particle size was obtained using a POE with DP=10, HLB=14.7. The POE surfactants had little effect on the selective production of F1. The formation of filamentous pellets depended on the surfactant HLB value, and F1 enzymes were produced most efficiently using Tween 20.
[Mh] Termos MeSH primário: Aspergillus oryzae/enzimologia
Tensoativos/farmacologia
beta-Frutofuranosidase/biossíntese
[Mh] Termos MeSH secundário: Interações Hidrofóbicas e Hidrofílicas/efeitos dos fármacos
Oligossacarídeos/biossíntese
Oligossacarídeos/isolamento & purificação
Tamanho da Partícula
Óleos Vegetais/farmacologia
Polietilenoglicóis/farmacologia
Polissorbatos/farmacologia
beta-Frutofuranosidase/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligosaccharides); 0 (Plant Oils); 0 (Polysorbates); 0 (Surface-Active Agents); 0 (fructooligosaccharide); 30IQX730WE (Polyethylene Glycols); 9004-98-2 (polyethylene glycol oleyl ether); EC 3.2.1.26 (beta-Fructofuranosidase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE



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