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[PMID]:28572155
[Au] Autor:Meghraoui-Kheddar A; Pierre A; Sellami M; Audonnet S; Lemaire F; Le Naour R
[Ad] Endereço:EA4683, SFR CAP-Santé, Université de Reims Champagne-Ardenne, Reims, France; and.
[Ti] Título:Elastin receptor (S-gal) occupancy by elastin peptides modulates T-cell response during murine emphysema.
[So] Source:Am J Physiol Lung Cell Mol Physiol;313(3):L534-L547, 2017 Sep 01.
[Is] ISSN:1522-1504
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chronic obstructive pulmonary disease and emphysema are associated with increased elastin peptides (EP) production because of excessive breakdown of lung connective tissue. We recently reported that exposure of mice to EP elicited hallmark features of emphysema. EP effects are largely mediated through a receptor complex that includes the elastin-binding protein spliced-galactosidase (S-gal). In previous studies, we established a correlation between cytokine production and S-gal protein expression in EP-treated immune cells. In this study, we investigated the S-gal-dependent EP effects on T-helper (Th) and T-cytotoxic (Tc) responses during murine EP-triggered pulmonary inflammation. C57BL/6J mice were endotracheally instilled with the valine-glycine-valine-alanine-proline-glycine (VGVAPG) elastin peptide, and, 21 days after treatment, local and systemic T-lymphocyte phenotypes were analyzed at cytokine and transcription factor expression levels by multicolor flow cytometry. Exposure of mice to the VGVAPG peptide resulted in a significant increase in the proportion of the CD4 and CD8 T cells expressing the cytokines IFN-γ or IL-17a and the transcription factors T-box expressed in T cells or retinoic acid-related orphan receptor-γt (RORγt) without effects on IL-4 and Gata-binding protein 3 to DNA sequence [A/T]GATA[A/G] expression. These effects were maximized when each T-cell subpopulation was challenged ex vivo with EP, and they were inhibited in vivo when an analogous peptide antagonizing the EP/S-gal interactions was instilled together with the VGVAPG peptide. This study demonstrates that, during murine emphysema, EP-S-gal interactions contribute to a Th-1 and Th-17 proinflammatory T-cell response combined with a Tc-1 response. Our study also highlights the S-gal receptor as a putative pharmacological target to modulate such an immune response.
[Mh] Termos MeSH primário: Elastina/metabolismo
Galactosidases/metabolismo
Peptídeos/metabolismo
Enfisema Pulmonar/imunologia
Enfisema Pulmonar/patologia
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Líquido da Lavagem Broncoalveolar
Linfócitos T CD8-Positivos/imunologia
Elastina/química
Feminino
Galactosidases/antagonistas & inibidores
Interferon gama/metabolismo
Interleucina-17/metabolismo
Linfonodos/patologia
Contagem de Linfócitos
Camundongos Endogâmicos C57BL
Modelos Biológicos
Elastase Pancreática/metabolismo
Peptídeos/química
Baço/patologia
Sus scrofa
Linfócitos T Citotóxicos/efeitos dos fármacos
Linfócitos T Citotóxicos/imunologia
Células Th1/imunologia
Células Th17/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-17); 0 (Peptides); 82115-62-6 (Interferon-gamma); 9007-58-3 (Elastin); EC 3.2.1.- (Galactosidases); EC 3.4.21.36 (Pancreatic Elastase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.1152/ajplung.00465.2016


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[PMID]:28480354
[Au] Autor:Im E; Ae A; Bn U; Po U
[Ad] Endereço:Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka.
[Ti] Título:IMMUNO-MODULATORY PROPERTIES OF PREBIOTICS EXTRACTED FROM .
[So] Source:Afr J Tradit Complement Altern Med;13(6):11-17, 2016.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: , commonly called bitter-leaf, is widely consumed in many parts of Africa, and Nigeria, in particular. The leaf extract has been reported to have antimicrobial, anti-plasmodial, anti-helminthic, as well as prebiotic properties, but its immuno-modulatory effects have not been well-studied, neither have the prebiotics been identified. This study evaluated the immuno-modulatory properties of the aqueous leaf extract and identified the prebiotic components. METHODS: The immuno-modulatory potential was evaluated by monitoring the effects of oral administration of the extract on immunological, haematological and lipid profiles of , while the prebiotic components were identified by thin layer chromatography (TLC), following liquid-liquid fractionation of the extract. RESULTS: Consumption of the extract caused significant increases in CD4+-, white blood cell-, total lymphocyte- and high density lipid (HDL) counts; decreases in low density lipid (LDL) and triglycerides and no significant effect on haemoglobin (Hb) and packed cell volume (PCV) in the blood of test animals. The water-soluble fraction of the extract contained most of the phyto-constituents of the extract and Thin Layer Chromatographic analysis of the fraction revealed the presence of fructo-oligosaccharide and galacto-oligosaccharide prebiotics. CONCLUSION: The results from this study have shown that the aqueous leaf extract of has positive immune-modulatory and haematologic effects and contains some important prebiotic compounds.
[Mh] Termos MeSH primário: Fatores Imunológicos/farmacologia
Extratos Vegetais/farmacologia
Folhas de Planta/química
Prebióticos/análise
Vernonia/química
[Mh] Termos MeSH secundário: Animais
Linfócitos T CD4-Positivos/efeitos dos fármacos
Cromatografia em Camada Delgada
Galactosidases/análise
Hematócrito
Hemoglobinas/efeitos dos fármacos
Leucócitos/efeitos dos fármacos
Lipoproteínas HDL/efeitos dos fármacos
Lipoproteínas LDL/efeitos dos fármacos
Contagem de Linfócitos
Oligossacarídeos/análise
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hemoglobins); 0 (Immunologic Factors); 0 (Lipoproteins, HDL); 0 (Lipoproteins, LDL); 0 (Oligosaccharides); 0 (Plant Extracts); 0 (Prebiotics); 0 (fructooligosaccharide); EC 3.2.1.- (Galactosidases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v13i6.3


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[PMID]:27685756
[Au] Autor:Godoy AS; Camilo CM; Kadowaki MA; Muniz HD; Espirito Santo M; Murakami MT; Nascimento AS; Polikarpov I
[Ad] Endereço:Departamento de Física em São Carlos, Universidade de São Paulo, Brazil.
[Ti] Título:Crystal structure of ß1→6-galactosidase from Bifidobacterium bifidum S17: trimeric architecture, molecular determinants of the enzymatic activity and its inhibition by α-galactose.
[So] Source:FEBS J;283(22):4097-4112, 2016 Nov.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In a search for better comprehension of ß-galactosidase function and specificity, we solved the crystal structures of the GH42 ß-galactosidase BbgII from Bifidobacterium bifidum S17, a well-adapted probiotic microorganism from the human digestive tract, and its complex with d-α-galactose. BbgII is a three-domain molecule that forms barrel-shaped trimers in solution. BbgII interactions with d-α-galactose, a competitive inhibitor, showed a number of residues that are involved in the coordination of ligands. A combination of site-directed mutagenesis of these amino acid residues with enzymatic activity measurements confirmed that Glu161 and Glu320 are fundamental for catalysis and their substitution by alanines led to catalytically inactive mutants. Mutation Asn160Ala resulted in a two orders of magnitude decrease of the enzyme k without significant modification in its K , whereas mutations Tyr289Phe and His371Phe simultaneously decreased k and increased K values. Enzymatic activity of Glu368Ala mutant was too low to be detected. Our docking and molecular dynamics simulations showed that the enzyme recognizes and tightly binds substrates with ß1→6 and ß1→3 bonds, while binding of the substrates with ß1→4 linkages is less favorable. DATABASE: Structural data are available in the PDB under the accession numbers 4UZS and 4UCF.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Bifidobacterium bifidum/enzimologia
Galactose/metabolismo
Galactosidases/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos/química
Aminoácidos/genética
Aminoácidos/metabolismo
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Bifidobacterium bifidum/genética
Sítios de Ligação/genética
Biocatálise/efeitos dos fármacos
Domínio Catalítico
Cristalografia por Raios X
Galactose/química
Galactose/farmacologia
Galactosidases/química
Galactosidases/genética
Cinética
Conformação Molecular
Simulação de Dinâmica Molecular
Mutação de Sentido Incorreto
Domínios Proteicos
Multimerização Proteica
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Bacterial Proteins); EC 3.2.1.- (Galactosidases); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160930
[St] Status:MEDLINE
[do] DOI:10.1111/febs.13908


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[PMID]:27451952
[Au] Autor:Rimlumduan T; Hua YL; Tanaka T; Ketudat Cairns JR
[Ad] Endereço:School of Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand; Center for Biomolecular Structure, Function and Application, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
[Ti] Título:Structure of a plant ß-galactosidase C-terminal domain.
[So] Source:Biochim Biophys Acta;1864(10):1411-8, 2016 10.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Most plant ß-galactosidases, which belong to glycoside hydrolase family 35, have a C-terminal domain homologous to animal galactose and rhamnose-binding lectins. To investigate the structure and function of this domain, the C-terminal domain of the rice (Oryza sativa L.) ß-galactosidase 1 (OsBGal1 Cter) was expressed in Escherichia coli and purified to homogeneity. The free OsBGal1 Cter is monomeric with a native molecular weight of 15kDa. NMR spectroscopy indicated that OsBGal1 Cter comprises five ß-strands and one α-helix. The structure of this domain is similar to lectin domains from animals, but loops A and C of OsBGal1 Cter are longer than the corresponding loops from related animal lectins with known structures. In addition, loop A of OsBGal1 Cter was not well defined, suggesting it is flexible. Although OsBGal1 Cter was predicted to be a galactose/rhamnose-binding domain, binding with rhamnose, galactose, glucose, ß-1,4-d-galactobiose and raffinose could not be observed in NMR experiments.
[Mh] Termos MeSH primário: Oryza/metabolismo
beta-Galactosidase/química
beta-Galactosidase/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sítios de Ligação
Escherichia coli/metabolismo
Galactose/química
Galactosidases/química
Glucose/química
Lectinas/química
Espectroscopia de Ressonância Magnética/métodos
Modelos Moleculares
Peso Molecular
Conformação Proteica em alfa-Hélice
Domínios Proteicos
Ramnose/química
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lectins); EC 3.2.1.- (Galactosidases); EC 3.2.1.23 (beta-Galactosidase); IY9XDZ35W2 (Glucose); QN34XC755A (Rhamnose); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160726
[St] Status:MEDLINE


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[PMID]:27090759
[Au] Autor:Kindaichi T; Yamaoka S; Uehara R; Ozaki N; Ohashi A; Albertsen M; Nielsen PH; Nielsen JL
[Ad] Endereço:Department of Civil and Environmental Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashihiroshima, 739-8527 Japan Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220 Aalborg E, Denmark.
[Ti] Título:Phylogenetic diversity and ecophysiology of Candidate phylum Saccharibacteria in activated sludge.
[So] Source:FEMS Microbiol Ecol;92(6):fiw078, 2016 Jun.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Candidate phylum Saccharibacteria (former TM7) are abundant and widespread in nature, but little is known about their ecophysiology and detailed phylogeny. In this study phylogeny, morphology and ecophysiology of Saccharibacteria were investigated in activated sludge from nine wastewater treatment plants (WWTPs) from Japan and Denmark using the full-cycle 16S rRNA approach in combination with microautoradiography (MAR) and fluorescence in situ hybridization (FISH). Phylogenetic analysis showed that Saccharibacteria from all WWTPs were evenly distributed within subdivision 1 and 3 and in a distinct phylogenetic clade. Three probes were designed for the distinct saccharibacterial groups, and revealed morphotypes representing thin filaments, thick filaments and rods/cocci. MAR-FISH results showed that most probe-defined Saccharibacteria utilized glucose under aerobic-, nitrate reducing- and anaerobic conditions. Some Saccharibacteria also utilized N-acetylglucosamine, oleic acid, amino acids and butyrate, which are not predicted from available genomes so far. In addition, some filamentous Saccharibacteria exhibited ß-galactosidase and lipase activities determined using a combination of enzyme-labeled fluorescence and FISH (ELF-FISH). No uptake of acetate, propionate, pyruvate, glycerol and ethanol was observed. These results indicate that Saccharibacteria is a phylogenetically diverse group and play a role in the degradation of various organic compounds as well as sugar compounds under aerobic-, nitrate reducing- and anaerobic conditions.
[Mh] Termos MeSH primário: Bactérias
Metabolismo Energético/genética
Esgotos/microbiologia
[Mh] Termos MeSH secundário: Autorradiografia
Bactérias/classificação
Bactérias/genética
Bactérias/metabolismo
DNA Bacteriano/genética
Dinamarca
Galactosidases/metabolismo
Glucose/metabolismo
Hibridização in Situ Fluorescente/métodos
Japão
Lipase/metabolismo
Filogenia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (Sewage); EC 3.1.1.3 (Lipase); EC 3.2.1.- (Galactosidases); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160420
[St] Status:MEDLINE


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[PMID]:26891970
[Au] Autor:Parvizi-Bahktar P; Mendez-Campos J; Raju L; Khalique NA; Jubeli E; Larsen H; Nicholson D; Pungente MD; Fyles TM
[Ad] Endereço:Department of Chemistry, University of Victoria, P.O. Box 3065, Stn CSC, Victoria, BC V8W 3V6, Canada. tmf@uvic.ca.
[Ti] Título:Structure-activity correlation in transfection promoted by pyridinium cationic lipids.
[So] Source:Org Biomol Chem;14(11):3080-90, 2016 Mar 21.
[Is] ISSN:1477-0539
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The efficiency of the transfection of a plasmid DNA encoding a galactosidase promoted by a series of pyridinium lipids in mixtures with other cationic lipids and neutral lipids was assessed in CHO-K1 cells. We identify key molecular parameters of the lipids in the mixture - clog P, lipid length, partial molar volume - to predict the morphology of the lipid-DNA lipoplex and then correlate these same parameters with transfection efficiency in an in vitro assay. We define a Transfection Index that provides a linear correlation with normalized transfection efficiency over a series of 90 different lipoplex compositions. We also explore the influence of the same set of molecular parameters on the cytotoxicity of the formulations.
[Mh] Termos MeSH primário: DNA/administração & dosagem
Galactosidases/genética
Lipídeos/química
Plasmídeos/administração & dosagem
Compostos de Piridínio/química
Transfecção
[Mh] Termos MeSH secundário: Animais
Células CHO
Cátions/química
Cátions/toxicidade
Sobrevivência Celular/efeitos dos fármacos
Cricetulus
DNA/genética
Lipídeos/toxicidade
Plasmídeos/genética
Compostos de Piridínio/toxicidade
Transfecção/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cations); 0 (Lipids); 0 (Pyridinium Compounds); 9007-49-2 (DNA); EC 3.2.1.- (Galactosidases)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160220
[St] Status:MEDLINE
[do] DOI:10.1039/c6ob00041j


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[PMID]:26717544
[Au] Autor:Thonhofer M; Gonzalez Santana A; Fischer R; Torvisco Gomez A; Saf R; Schalli M; Stütz AE; Withers SG
[Ad] Endereço:Glycogroup, Institute for Organic Chemistry, Graz University of Technology, Stremayrgasse 9, A-8010 Graz, Austria.
[Ti] Título:5-Fluoro derivatives of 4-epi-isofagomine as D-galactosidase inhibitors and potential pharmacological chaperones for GM1-gangliosidosis as well as Fabry's disease.
[So] Source:Carbohydr Res;420:6-12, 2016 Feb.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Electrophilic fluorination of an exocyclic methoxymethylene enol ether derived from N-tert-butyloxycarbonyl-1,5-dideoxy-1,5-imino-3,4-O-isopropylidene-D-erythro-pent-2-ulose (11) provided the 5-fluoro derivative of the powerful ß-galactosidase inhibitor 4-epi-isofagomine (8). This structural alteration, in combination with N-alkylation, led to considerably improved α-galactosidase selectivity. New compounds may serve as leads en route to new pharmacological chaperones for Fabry's disease.
[Mh] Termos MeSH primário: Inibidores Enzimáticos/síntese química
Galactosidases/antagonistas & inibidores
Imino Piranoses/síntese química
[Mh] Termos MeSH secundário: Inibidores Enzimáticos/química
Inibidores Enzimáticos/farmacologia
Doença de Fabry/tratamento farmacológico
Doença de Fabry/enzimologia
Gangliosidose GM1/tratamento farmacológico
Gangliosidose GM1/enzimologia
Halogenação
Seres Humanos
Imino Piranoses/química
Imino Piranoses/farmacologia
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Imino Pyranoses); 0 (isofagomine); EC 3.2.1.- (Galactosidases)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151231
[St] Status:MEDLINE


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[PMID]:26644389
[Au] Autor:Front S; Gallienne E; Charollais-Thoenig J; Demotz S; Martin OR
[Ad] Endereço:Institut de Chimie Organique et Analytique (ICOA), UMR 7311, Université d'Orléans, Centre National de la Recherche Scientifique (CNRS), Rue de Chartres, 45067, Orléans, France.
[Ti] Título:N-Alkyl-, 1-C-Alkyl-, and 5-C-Alkyl-1,5-dideoxy-1,5-imino-(L)-ribitols as Galactosidase Inhibitors.
[So] Source:ChemMedChem;11(1):133-41, 2016 Jan 05.
[Is] ISSN:1860-7187
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A series of 1,5-dideoxy-1,5-imino-(l)-ribitol (DIR) derivatives carrying alkyl or functionalized alkyl groups were prepared and investigated as glycosidase inhibitors. These compounds were designed as simplified 4-epi-isofagomine (4-epi-IFG) mimics and were expected to behave as selective inhibitors of ß-galactosidases. All compounds were indeed found to be highly selective for ß-galactosidases versus α-glycosidases, as they generally did not inhibit coffee bean α-galactosidase or other α-glycosidases. Some compounds were also found to be inhibitors of almond ß-glucosidase. The N-alkyl DIR derivatives were only modest inhibitors of bovine ß-galactosidase, with IC50 values in the 30-700 µM range. Likewise, imino-L-ribitol substituted at the C1 position was found to be a weak inhibitor of this enzyme. In contrast, alkyl substitution at C5 resulted in enhanced ß-galactosidase inhibitory activity by a factor of up to 1000, with at least six carbon atoms in the alkyl substituent. Remarkably, the 'pseudo-anomeric' configuration in this series does not appear to play a role. Human lysosomal ß-galactosidase from leukocyte lysate was, however, poorly inhibited by all iminoribitol derivatives tested (IC50 values in the 100 µM range), while 4-epi-IFG was a good inhibitor of this enzyme. Two compounds were evaluated as pharmacological chaperones for a GM1-gangliosidosis cell line (R301Q mutation) and were found to enhance the mutant enzyme activity by factors up to 2.7-fold.
[Mh] Termos MeSH primário: Inibidores Enzimáticos/química
Inibidores Enzimáticos/farmacologia
Galactosidases/antagonistas & inibidores
Ribitol/análogos & derivados
Ribitol/farmacologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Relação Dose-Resposta a Droga
Inibidores Enzimáticos/síntese química
Galactosidases/metabolismo
Seres Humanos
Lisossomos/enzimologia
Conformação Molecular
Ribitol/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 488-81-3 (Ribitol); EC 3.2.1.- (Galactosidases)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151209
[St] Status:MEDLINE
[do] DOI:10.1002/cmdc.201500485


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[PMID]:25394540
[Au] Autor:Bakunina IY; Balabanova LA; Pennacchio A; Trincone A
[Ad] Endereço:a G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences , Vladivostok , Russia and.
[Ti] Título:Hooked on α-d-galactosidases: from biomedicine to enzymatic synthesis.
[So] Source:Crit Rev Biotechnol;36(2):233-45, 2016.
[Is] ISSN:1549-7801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:α-d-Galactosidases (EC 3.2.1.22) are enzymes employed in a number of useful bio-based applications. We have depicted a comprehensive general survey of α-d-galactosidases from different origin with special emphasis on marine example(s). The structures of natural α-galactosyl containing compounds are described. In addition to 3D structures and mechanisms of action of α-d-galactosidases, different sources, natural function and genetic regulation are also covered. Finally, hydrolytic and synthetic exploitations as free or immobilized biocatalysts are reviewed. Interest in the synthetic aspects during the next years is anticipated for access to important small molecules by green technology with an emphasis on alternative selectivity of this class of enzymes from different sources.
[Mh] Termos MeSH primário: Biocatálise
Biotecnologia
Galactosidases
[Mh] Termos MeSH secundário: Animais
Organismos Aquáticos/enzimologia
Enzimas Imobilizadas
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Enzymes, Immobilized); EC 3.2.1.- (Galactosidases)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141115
[St] Status:MEDLINE
[do] DOI:10.3109/07388551.2014.949618


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[PMID]:26505308
[Au] Autor:Zhang Q; Xiao Q; Xu J; Tong Y; Wen J; Chen X; Wei L
[Ad] Endereço:College of Plant Protection and Horticulture, Yangzhou University, Yangzhou 225009, PR China. Electronic address: zqx817@126.com.
[Ti] Título:Effect of retS gene on antibiotics production in Pseudomonas fluorescens FD6.
[So] Source:Microbiol Res;180:23-9, 2015 Nov.
[Is] ISSN:1618-0623
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A hybrid sensor kinase termed RetS (regulator of exopolysaccharide and Type III secretion) controls expression of numerous genes in Pseudomonas aeruginosa. To investigate the function of RetS in P. fluorescens FD6, the retS gene was disrupted. Genetic inactivation of retS resulted in enhanced production of 2, 4-diacetylphloroglucinol, pyrrolnitrin, and pyoluteorin. The retS mutant also exhibited significant increase in phlA-lacZ, prnA-lacZ, and pltA-lacZ transcription levels, influencing expression levels of the small regulatory RNAs RsmX and RsmZ. In the gacSretS double mutant, all the phenotypic changes caused by the retS deletion were reversed to the level of gacS single mutant. Furthermore, the retS mutation drastically elevated biofilm formation and improved the colonization ability of strain FD6 on wheat rhizospheres. Based on these results, we proposed that RetS negatively controlled the production of antibiotics through the Gac/Rsm pathway in P. fluorescens FD6.
[Mh] Termos MeSH primário: Antibacterianos/biossíntese
Pseudomonas fluorescens/genética
Pseudomonas fluorescens/metabolismo
Fatores de Virulência/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Biofilmes
Galactosidases/metabolismo
Inativação Gênica
Meristema/microbiologia
Mutação
Fenóis/metabolismo
Floroglucinol/análogos & derivados
Floroglucinol/metabolismo
Pirróis/metabolismo
Pirrolnitrina/metabolismo
Rizosfera
Triticum/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Phenols); 0 (Pyrroles); 0 (Virulence Factors); 0 (pyoluteorin); 8XV4YYO3WN (2,4-diacetylphloroglucinol); DHD7FFG6YS (Phloroglucinol); EC 3.2.1.- (Galactosidases); N0P24B6EDQ (Pyrrolnitrin)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:151028
[Lr] Data última revisão:
151028
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151028
[St] Status:MEDLINE



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