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[PMID]:28743272
[Au] Autor:Havlik D; Brandt U; Bohle K; Fleißner A
[Ad] Endereço:Division of Pharmaceutical Biotechnology, Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Inhoffenstr. 7, Braunschweig, 38124, Germany.
[Ti] Título:Establishment of Neurospora crassa as a host for heterologous protein production using a human antibody fragment as a model product.
[So] Source:Microb Cell Fact;16(1):128, 2017 Jul 25.
[Is] ISSN:1475-2859
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Filamentous fungi are commonly used as production hosts for bulk enzymes in biotechnological applications. Their robust and quick growth combined with their ability to secrete large amounts of protein directly into the culture medium makes fungi appealing organisms for the generation of novel production systems. The red bread mold Neurospora crassa has long been established as a model system in basic research. It can be very easily genetically manipulated and a wealth of molecular tools and mutants are available. In addition, N. crassa is very fast growing and non-toxic. All of these features point to a high but so far untapped potential of this fungus for biotechnological applications. In this study, we used genetic engineering and bioprocess development in a design-build-test-cycle process to establish N. crassa as a production host for heterologous proteins. RESULTS: The human antibody fragment HT186-D11 was fused to a truncated version of the endogenous enzyme glucoamylase (GLA-1), which served as a carrier protein to achieve secretion into the culture medium. A modular expression cassette was constructed and tested under the control of different promoters. Protease activity was identified as a major limitation of the production strain, and the effects of different mutations causing protease deficiencies were compared. Furthermore, a parallel bioreactor system (1 L) was employed to develop and optimize a production process, including the comparison of different culture media and cultivation parameters. After successful optimization of the production strain and the cultivation conditions an exemplary scale up to a 10 L stirred tank reactor was performed. CONCLUSIONS: The data of this study indicate that N. crassa is suited for the production and secretion of heterologous proteins. Controlling expression by the optimized promoter Pccg1nr in a fourfold protease deletion strain resulted in the successful secretion of the heterologous product with estimated yields of 3 mg/L of the fusion protein. The fungus could easily be cultivated in bioreactors and a first scale-up was successful. The system holds therefore much potential, warranting further efforts in optimization.
[Mh] Termos MeSH primário: Fragmentos de Imunoglobulinas/metabolismo
Neurospora crassa/metabolismo
[Mh] Termos MeSH secundário: Reatores Biológicos
Meios de Cultura/química
Glucana 1,4-alfa-Glucosidase/genética
Seres Humanos
Concentração de Íons de Hidrogênio
Fragmentos de Imunoglobulinas/genética
Plasmídeos/genética
Plasmídeos/metabolismo
Regiões Promotoras Genéticas
Proteínas Recombinantes de Fusão/biossíntese
Proteínas Recombinantes de Fusão/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Immunoglobulin Fragments); 0 (Recombinant Fusion Proteins); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1186/s12934-017-0734-5


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[PMID]:27770419
[Au] Autor:Milosavic NB; Prodanovic RM; Velickovic D; Dimitrijevic A
[Ad] Endereço:Division of Experimental Therapeutics, Department of Medicine, Columbia University, 630 West 168th Street, P&S 8-436, New York, NY, 10032, USA. nm2729@columbia.edu.
[Ti] Título:Macroporous Poly(GMA-co-EGDMA) for Enzyme Stabilization.
[So] Source:Methods Mol Biol;1504:139-147, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One of the most used procedures for enzyme stabilization is immobilization. Although immobilization on solid supports has been pursued since the 1950s, there are no general rules for selecting the best support for a giving application. A macroporous copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate (poly (GMA-co-EGDMA)) is a carrier consisting of macroporous beads for immobilizing enzymes of industrial potential for the production of fine chemicals and pharmaceuticals.
[Mh] Termos MeSH primário: Aspergillus niger/enzimologia
Reagentes para Ligações Cruzadas/química
Enzimas Imobilizadas/química
Etilenoglicóis/química
Glucana 1,4-alfa-Glucosidase/química
Glutaral/química
Metacrilatos/química
[Mh] Termos MeSH secundário: Aspergillus niger/química
Aspergillus niger/metabolismo
Estabilidade Enzimática
Enzimas Imobilizadas/metabolismo
Óxido de Etileno/química
Glucana 1,4-alfa-Glucosidase/metabolismo
Oxirredução
Porosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Enzymes, Immobilized); 0 (Ethylene Glycols); 0 (Methacrylates); 0 (poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase); JJH7GNN18P (Ethylene Oxide); T3C89M417N (Glutaral)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


  3 / 1666 MEDLINE  
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[PMID]:28727742
[Au] Autor:Jung YS; Lee BH; Yoo SH
[Ad] Endereço:Department of Food Science and Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong University, Seoul, South Korea.
[Ti] Título:Physical structure and absorption properties of tailor-made porous starch granules produced by selected amylolytic enzymes.
[So] Source:PLoS One;12(7):e0181372, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Porous starch granules (PSGs) with various pores and cavity sizes were prepared by amylolysis enzymes. The greatest hydrolysis rate on corn starch granule was observed with α-amylase, followed by gluco- and ß-amylases. Temperature increase enhanced glucoamylase reaction rate more drastically than other enzyme treatments. Final hydrolysis level with glucoamylase reached to 66.9%, close to 67.5% of α-amylolysis. The α-amylase-treated PSGs displayed the greatest pore size and ratio of cavity-to-granule diameters. Gelatinization onset temperatures of PSGs increased to 72.1 (α-), 68.7 (ß-), and 68.1°C (gluco-amylolysis) after 8 h; enthalpy changes of ß- and gluco-amylase-treated PSGs increased to 13.4, and 13.1 J/g but α-amylase-treated one showed slightly reduced value of 8.5 J/g. Water holding capacities of PSGs were 209.7 (α-), 94.6 (ß-), and 133.8% (gluco-amylolysis), and the untreated control had 89.1%; oil holding capacities of them showed 304.5, 182.7, and 211.5%, respectively, while the untreated control had 161.8%. Thus, enzyme types and their reaction conditions can be applied to generate desirable cavity and pore sizes in starch granules. This biocatalytic approach could contribute to develop tailor-made PSGs with distinct internal structure for specific uses in wide range of food, pharmaceutical and other industrial applications.
[Mh] Termos MeSH primário: Glucana 1,4-alfa-Glucosidase/metabolismo
Amido/química
alfa-Amilases/metabolismo
beta-Amilase/metabolismo
[Mh] Termos MeSH secundário: Absorção Fisico-Química
Aspergillus niger/enzimologia
Bacillus licheniformis/enzimologia
Biocatálise
Varredura Diferencial de Calorimetria
Hordeum/enzimologia
Hidrólise
Microscopia Confocal
Microscopia Eletrônica de Varredura
Porosidade
Óleo de Soja/química
Amido/metabolismo
Temperatura Ambiente
Água/química
Zea mays/química
alfa-Amilases/química
beta-Amilase/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water); 8001-22-7 (Soybean Oil); 9005-25-8 (Starch); EC 3.2.1.1 (alpha-Amylases); EC 3.2.1.2 (beta-Amylase); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181372


  4 / 1666 MEDLINE  
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[PMID]:28660762
[Au] Autor:Xia L; Bai Y; Mu W; Wang J; Xu X; Jin Z
[Ad] Endereço:State Key laboratory of Food Science and Technology, Jiangnan University , Wuxi, Jiangsu Province 214122, China.
[Ti] Título:Efficient Synthesis of Glucosyl-ß-Cyclodextrin from Maltodextrins by Combined Action of Cyclodextrin Glucosyltransferase and Amyloglucosidase.
[So] Source:J Agric Food Chem;65(29):6023-6029, 2017 Jul 26.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Instead of ß-cyclodextrin (ß-CD), branched ß-CDs have been increasingly used in many aspects as they possess better solubility and higher bioadaptability. But most commercialized branched ß-CDs were chemically synthesized. Thus, the glucosyl-ß-cyclodextrin (G -ß-CD) prepared via enzymatic approach could be a nice substitute. However, the yield of G -ß-CD was low. Here, we reported a controlled two-step reaction to efficiently prepare G -ß-CD from maltodextrins by ß-cyclodextrin glucosyltransferase (ß-CGTase) and amyloglucosidase (AG). Compared to the single ß-CGTase reaction, controlled two-step reaction caused a yield increase of G -ß-CD by 130%. Additionally, the percentage of G -ß-CD was enhanced from 2.4% to 24.0% and the side products α-CD and γ-CD were hydrolyzed because of the coupling activity of ß-CGTase. Thus, this controlled two-step reaction might be an efficient approach for industrial production of pure G -ß-CD.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Proteínas Fúngicas/química
Glucana 1,4-alfa-Glucosidase/química
Glucosiltransferases/química
Polissacarídeos/química
beta-Ciclodextrinas/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Biocatálise
Proteínas Fúngicas/metabolismo
Glucana 1,4-alfa-Glucosidase/metabolismo
Estrutura Molecular
Polissacarídeos/metabolismo
Rhizopus/enzimologia
Thermoanaerobacter/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Fungal Proteins); 0 (Polysaccharides); 0 (beta-Cyclodextrins); 7CVR7L4A2D (maltodextrin); EC 2.4.1.- (Glucosyltransferases); EC 2.4.1.19 (cyclomaltodextrin glucanotransferase); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02079


  5 / 1666 MEDLINE  
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[PMID]:28650980
[Au] Autor:Hu W; Li W; Chen H; Liu J; Wang S; Chen J
[Ad] Endereço:Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou city, Gansu Province, China.
[Ti] Título:Changes in transcript levels of starch hydrolysis genes and raising citric acid production via carbon ion irradiation mutagenesis of Aspergillus niger.
[So] Source:PLoS One;12(6):e0180120, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The filamentous ascomycete Aspergillus niger is well known for its ability to accumulate citric acid for the hydrolysis of starchy materials. To improve citric acid productivity, heavy ion beam mutagenesis was utilized to produce mutant A.niger strains with enhanced production of citric acid in this work. It was demonstrated that a mutant HW2 with high concentration of citric acid was isolated after carbon ion irradiation with the energy of 80Mev/µ, which was obvious increase higher than the original strain from liquefied corn starch as a feedstock. More importantly, with the evidence from the expression profiles of key genes and enzyme activity involved in the starch hydrolysis process between original strain and various phenotype mutants, our results confirmed that different transcript levels of key genes involving in starch hydrolysis process between original strain and mutants could be a significant contributor to different citric acid concentration in A.niger, such as, amyR and glaA, which therefore opened a new avenue for constructing genetically engineered A.niger mutants for high-yield citric acid accumulation in the future. As such, this work demonstrated that heavy ion beam mutagenesis presented an efficient alternative strategy to be developed to generate various phenotype microbe species mutants for functional genes research.
[Mh] Termos MeSH primário: Aspergillus niger/genética
Aspergillus niger/metabolismo
Ácido Cítrico/metabolismo
Genes Fúngicos
Mutagênese
Amido/metabolismo
[Mh] Termos MeSH secundário: Aspergillus niger/efeitos da radiação
Carbono
Fermentação
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Genes Fúngicos/efeitos da radiação
Glucana 1,4-alfa-Glucosidase/genética
Glucana 1,4-alfa-Glucosidase/metabolismo
Íons Pesados
Hidrólise
Fenótipo
Transativadores/genética
Transativadores/metabolismo
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Trans-Activators); 0 (amyR protein, Aspergillus); 2968PHW8QP (Citric Acid); 7440-44-0 (Carbon); 9005-25-8 (Starch); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180120


  6 / 1666 MEDLINE  
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[PMID]:28592009
[Au] Autor:Luo JH; Qiu WJ; Fang D; Ye J; Han LS; Zhang HW; Yu YG; Liang LL; Gu XF
[Ad] Endereço:Department of Pediatric Endocrinology and Genetics, Shanghai Institute of Pediatric Research, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
[Ti] Título:[Clinical and gene mutation analysis of three children with late-onset glycogen storage disease type â…¡ with hypertrophic cardiomyopathy].
[So] Source:Zhonghua Er Ke Za Zhi;55(6):423-427, 2017 Jun 02.
[Is] ISSN:0578-1310
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To investigate the clinical and laboratory features of three children with late-onset type â…¡ glycogen storage disease(GSD) who presented with hypertrophic cardiomyopathy and to analyze the effect of five mutations identified on the acid-α-glucosidase (GAA) activity and stability. Three cases of children with muscle weakness were included in this study.GAA activity was analyzed in Dried Blood Spot of the patients.DNA was extracted from peripheral blood in all the patients and their parents and subjected to polymerase chain reaction and directly sequencing of GAA gene.Five mutant pcDNA3.1-myc-his-GAA expression plasmids(p.G478R, p.P361L, p.P266S, p.Q323X, p.R672Q) were constructed and transient instantaneously transfected into 293T cells to analyze the enzyme activity and stability of GAA. All the three children had the onset of disease at 3 years or 1.5 years of age.They presented with developmental delay, muscle weakness and hypertrophic cardiomyopathy.GAA activity of 3 patients was 2.65, 3.55 and 1.51 pmol(punch·h)(8.00-98.02)respectively. Genetic analysis found 5 mutations (p.G478R, p. P361L, p. P266S, p. Q323X, p. R672Q), and all of these 3 cases had clinical manifestations and were diagnosed as late-onset type â…¡ glycogen storage disease.Five mutant pcDNA3.1-myc-his-GAA expression plasmids were transfected into 293T cells.Five mutant enzyme activities were found to be only 9.9%-22.5% of the wild-type enzyme activity and the protein expression of the five mutants was 32.0%-63.9% compared with the wild type. This study reports 3 children with late-onset GSD â…¡ accompanied by hypertrophic cardiomyopathy and compensatory stage of cardiac function in addition to limb muscle weakness.Five pathogenic mutations were identified, and these 5 mutations result in decreased GAA activity and GAA expression by in vitro functional analysis.
[Mh] Termos MeSH primário: Doença de Depósito de Glicogênio Tipo II/genética
Mutação
alfa-Glucosidases/genética
[Mh] Termos MeSH secundário: Idade de Início
Cardiomiopatia Hipertrófica/complicações
Criança
DNA
Testes Genéticos
Glucana 1,4-alfa-Glucosidase
Doença de Depósito de Glicogênio Tipo II/complicações
Seres Humanos
Debilidade Muscular
Reação em Cadeia da Polimerase
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA); EC 3.2.1.20 (alpha-Glucosidases); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0578-1310.2017.06.006


  7 / 1666 MEDLINE  
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[PMID]:28553713
[Au] Autor:Dura A; Rose DJ; Rosell CM
[Ad] Endereço:Institute of Agrochemistry and Food Technology (IATA), Spanish National Research Council (CSIC) , Avenida Agustin Escardino, 7, 46980 Paterna, Valencia, Spain.
[Ti] Título:Enzymatic Modification of Corn Starch Influences Human Fecal Fermentation Profiles.
[So] Source:J Agric Food Chem;65(23):4651-4657, 2017 Jun 14.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enzymatically modified starches have been widely used in food applications to develop new products, but information regarding digestion and fecal fermentation of these products is sparse. The objective of this study was to determine the fermentation properties of corn starch modified with α-amylase, amyloglucosidase, or cyclodextrin glycosyltransferase and the possible role of hydrolysis products. Samples differed in their digestibility and availability to be fermented by the microbiota, resulting in differences in microbial metabolites produced during in vitro fermentation. The presence or absence of hydrolysis products and gelatinization affected starch composition and subsequent metabolite production by the microbiota. Amyloglucosidase-treated starch led to the greatest production of short- and branched-chain fatty acid production by the microbiota. Results from this study could be taken into consideration to confirm the possible nutritional claims and potential health benefits of these starches as raw ingredients for food development.
[Mh] Termos MeSH primário: Fezes/microbiologia
Amido/química
Amido/metabolismo
[Mh] Termos MeSH secundário: Bactérias/classificação
Bactérias/isolamento & purificação
Bactérias/metabolismo
Digestão
Fermentação
Microbioma Gastrointestinal
Glucana 1,4-alfa-Glucosidase/química
Glucosiltransferases/química
Seres Humanos
Hidrólise
Zea mays/química
Zea mays/metabolismo
alfa-Amilases/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9005-25-8 (Starch); EC 2.4.1.- (Glucosyltransferases); EC 2.4.1.19 (cyclomaltodextrin glucanotransferase); EC 3.2.1.1 (alpha-Amylases); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170530
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01634


  8 / 1666 MEDLINE  
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[PMID]:28443839
[Au] Autor:Simsek M; Quezada-Calvillo R; Nichols BL; Hamaker BR
[Ad] Endereço:Whistler Center for Carbohydrate Research and Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
[Ti] Título:Phenolic compounds increase the transcription of mouse intestinal maltase-glucoamylase and sucrase-isomaltase.
[So] Source:Food Funct;8(5):1915-1924, 2017 May 24.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Diverse natural phenolic compounds show inhibition activity of intestinal α-glucosidases, which may constitute the molecular basis for their ability to control systemic glycemia. Additionally, phenolics can modify mRNA expression for proteins involved in nutritional, metabolic or immune processes. To explore the possibility that phenolics can regulate the mRNA expression, enzymatic activity, and protein synthesis/processing of intestinal Maltase-Glucoamylase (MGAM) and Sucrase-Isomaltase (SI), small intestinal explants from Balb/c mice were cultured for 24 h in the presence or absence of gallic acid, caffeic acid, and (+)-catechin at 0.1, 0.5, and 1 mM. We measured the levels of MGAM and SI mRNA expression by qRT-PCR, maltase and sucrase activities by a standard colorimetric method and the molecular size distribution of MGAM and SI proteins by western blotting. mRNA expression for MGAM was induced by the three phenolic compounds at 0.1 mM. mRNA expression for SI was induced by caffeic and gallic acids, but not by (+)-catechin. Caffeic acid was the most effective inducer of mRNA expression of these enzymes. Total maltase and sucrase activities were not affected by treatment with phenolics. The proportion of high molecular size forms of MGAM was significantly increased by two of the three phenolic compounds, but little effect was observed on SI proteins. Thus, changes in the protein synthesis/processing, affecting the proportions of the different molecular forms of MGAM, may account for the lack of correlation between mRNA expression and enzymatic activity.
[Mh] Termos MeSH primário: Glucana 1,4-alfa-Glucosidase/metabolismo
Intestinos/enzimologia
Fenol/farmacologia
Sacarase/metabolismo
alfa-Glucosidases/metabolismo
[Mh] Termos MeSH secundário: Animais
Glucana 1,4-alfa-Glucosidase/genética
Intestinos/efeitos dos fármacos
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Sacarase/genética
alfa-Glucosidases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
339NCG44TV (Phenol); EC 3.2.1.20 (alpha-Glucosidases); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase); EC 3.2.1.48 (Sucrase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE
[do] DOI:10.1039/c7fo00015d


  9 / 1666 MEDLINE  
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[PMID]:28371716
[Au] Autor:Wang P; Wu Q; Jiang X; Wang Z; Tang J; Xu Y
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Key Laboratory of Industrial Biotechnology, Ministry of Education, Synergetic Innovation Center of Food Safety and Nutrition, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China.
[Ti] Título:Bacillus licheniformis affects the microbial community and metabolic profile in the spontaneous fermentation of Daqu starter for Chinese liquor making.
[So] Source:Int J Food Microbiol;250:59-67, 2017 Jun 05.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chinese liquor is produced from spontaneous fermentation starter (Daqu) that provides the microbes, enzymes and flavors for liquor fermentation. To improve the flavor character of Daqu, we inoculated Bacillus licheniformis and studied the effect of this strain on the community structure and metabolic profile in Daqu fermentation. The microbial relative abundance changed after the inoculation, including the increase in Bacillus, Clavispora and Aspergillus, and the decrease in Pichia, Saccharomycopsis and some other genera. This variation was also confirmed by pure culture and coculture experiments. Seventy-three metabolites were identified during Daqu fermentation process. After inoculation, the average content of aromatic compounds were significantly enriched from 0.37mg/kg to 0.90mg/kg, and the average content of pyrazines significantly increased from 0.35mg/kg to 5.71mg/kg. The increase in pyrazines was positively associated with the metabolism of the inoculated Bacillus and the native genus Clavispora, because they produced much more pyrazines in their cocultures. Whereas the increase in aromatic compounds might be related to the change of in situ metabolic activity of several native genera, in particular, Aspergillus produced more aromatic compounds in cocultures with B. licheniformis. It indicated that the inoculation of B. licheniformis altered the flavor character of Daqu by both its own metabolic activity and the variation of in situ metabolic activity. Moreover, B. licheniformis inoculation influenced the enzyme activity of Daqu, including the significant increase in amylase activity (from 1.3gstarch/g/h to 1.7gstarch/g/h), and the significant decrease in glucoamylase activity (from 627.6mgglucose/g/h to 445.6mgglucose/g/h) and esterase activity (from 28.1mgethylcaproate/g/100h to 17.2mgethylcaproate/g/100h). These effects of inoculation were important factors for regulating the metabolism of microbial communities, hence for improving the flavor profile Daqu.
[Mh] Termos MeSH primário: Bebidas Alcoólicas/microbiologia
Aspergillus/isolamento & purificação
Bacillus licheniformis/metabolismo
Fermentação/fisiologia
Aromatizantes/metabolismo
Pichia/isolamento & purificação
Saccharomycetales/isolamento & purificação
[Mh] Termos MeSH secundário: Bebidas Alcoólicas/análise
Amilases/metabolismo
Glucana 1,4-alfa-Glucosidase/metabolismo
Metaboloma/fisiologia
Microbiota/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavoring Agents); EC 3.2.1.- (Amylases); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE


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[PMID]:28289929
[Au] Autor:Humbert P; Vemmer M; Giampà M; Bednarz H; Niehaus K; Patel AV
[Ad] Endereço:Faculty of Engineering and Mathematics, Fermentation and Formulation of Biologicals and Chemicals, Bielefeld University of Applied Sciences, Interaktion 1, 33619, Bielefeld, Germany.
[Ti] Título:Co-encapsulation of amyloglucosidase with starch and Saccharomyces cerevisiae as basis for a long-lasting CO release.
[So] Source:World J Microbiol Biotechnol;33(4):71, 2017 Apr.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:CO is known as a major attractant for many arthropod pests which can be exploited for pest control within novel attract-and-kill strategies. This study reports on the development of a slow-release system for CO based on calcium alginate beads containing granular corn starch, amyloglucosidase and Saccharomyces cerevisiae. Our aim was to evaluate the conditions which influence the CO release and to clarify the biochemical reactions taking place within the beads. The amyloglucosidase was immobilized with a high encapsulation efficiency of 87% in Ca-alginate beads supplemented with corn starch and S. cerevisiae biomass. The CO release from the beads was shown to be significantly affected by the concentration of amyloglucosidase and corn starch within the beads as well as by the incubation temperature. Beads prepared with 0.1 amyloglucosidase units/g matrix solution led to a long-lasting CO emission at temperatures between 6 and 25 °C. Starch degradation data correlated well with the CO release from beads during incubation and scanning electron microscopy micrographs visualized the degradation of corn starch granules by the co-encapsulated amyloglucosidase. By implementing MALDI-ToF mass spectrometry imaging for the analysis of Ca-alginate beads, we verified that the encapsulated amyloglucosidase converts starch into glucose which is immediately consumed by S. cerevisiae cells. When applied into the soil, the beads increased the CO concentration in soil significantly. Finally, we demonstrated that dried beads showed a CO production in soil comparable to the moist beads. The long-lasting CO -releasing beads will pave the way towards novel attract-and-kill strategies in pest control.
[Mh] Termos MeSH primário: Dióxido de Carbono/metabolismo
Glucana 1,4-alfa-Glucosidase/metabolismo
Saccharomyces cerevisiae/crescimento & desenvolvimento
Amido/química
[Mh] Termos MeSH secundário: Alginatos/química
Ácido Glucurônico/química
Ácidos Hexurônicos/química
Microesferas
Controle Biológico de Vetores/métodos
Solo/química
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Hexuronic Acids); 0 (Soil); 142M471B3J (Carbon Dioxide); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); 9005-25-8 (Starch); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-017-2237-2



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