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[PMID]:28780871
[Au] Autor:Li B; Meng J; Li L; Liu S; Wang T; Zhang G
[Ad] Endereço:University of Chinese Academy of Sciences , Beijing 100049, China.
[Ti] Título:Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas).
[So] Source:J Agric Food Chem;65(35):7764-7773, 2017 Sep 06.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:High glycogen levels in the Pacific oyster (Crassostrea gigas) contribute to its flavor, quality, and hardiness. Glycogenin (CgGN) is the priming glucosyltransferase that initiates glycogen biosynthesis. We characterized the full sequence and function of C. gigas CgGN. Three CgGN isoforms (CgGN-α, ß, and γ) containing alternative exon regions were isolated. CgGN expression varied seasonally in the adductor muscle and gonadal area and was the highest in the adductor muscle. Autoglycosylation of CgGN can interact with glycogen synthase (CgGS) to complete glycogen synthesis. Subcellular localization analysis showed that CgGN isoforms and CgGS were located in the cytoplasm. Additionally, a site-directed mutagenesis experiment revealed that the Tyr200Phe and Tyr202Phe mutations could affect CgGN autoglycosylation. This is the first study of glycogenin function in marine bivalves. These findings will improve our understanding of glycogen synthesis and accumulation mechanisms in mollusks. The data are potentially useful for breeding high-glycogen oysters.
[Mh] Termos MeSH primário: Crassostrea/genética
Glicogênio/biossíntese
Frutos do Mar/análise
[Mh] Termos MeSH secundário: Animais
Crassostrea/enzimologia
Crassostrea/metabolismo
Sistema da Enzima Desramificadora do Glicogênio/genética
Sistema da Enzima Desramificadora do Glicogênio/metabolismo
Glicogênio Sintase/genética
Glicogênio Sintase/metabolismo
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycogen Debranching Enzyme System); 0 (Protein Isoforms); 9005-79-2 (Glycogen); EC 2.4.1.11 (Glycogen Synthase); EC 2.4.1.25 (4 alpha-glucanotransferase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02720


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[PMID]:28576440
[Au] Autor:Smirnova J; Fernie AR; Spahn CMT; Steup M
[Ad] Endereço:Institute for Biochemistry and Biology, Department of Plant Physiology, University of Potsdam, Building 20, Karl-Liebknecht-Str. 20, 14461 Potsdam, Germany; Institute of Biophysics and Medical Physics of the Charité, Universitätsmedizin Berlin, Campus Berlin Mitte, 10117 Berlin, Germany; Max-Planck-
[Ti] Título:Photometric assay of maltose and maltose-forming enzyme activity by using 4-alpha-glucanotransferase (DPE2) from higher plants.
[So] Source:Anal Biochem;532:72-82, 2017 Sep 01.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maltose frequently occurs as intermediate of the central carbon metabolism of prokaryotic and eukaryotic cells. Various mutants possess elevated maltose levels. Maltose exists as two anomers, (α- and ß-form) which are rapidly interconverted without requiring enzyme-mediated catalysis. As maltose is often abundant together with other oligoglucans, selective quantification is essential. In this communication, we present a photometric maltose assay using 4-alpha-glucanotransferase (AtDPE2) from Arabidopsis thaliana. Under in vitro conditions, AtDPE2 utilizes maltose as glucosyl donor and glycogen as acceptor releasing the other hexosyl unit as free glucose which is photometrically quantified following enzymatic phosphorylation and oxidation. Under the conditions used, DPE2 does not noticeably react with other di- or oligosaccharides. Selectivity compares favorably with that of maltase frequently used in maltose assays. Reducing end interconversion of the two maltose anomers is in rapid equilibrium and, therefore, the novel assay measures total maltose contents. Furthermore, an AtDPE2-based continuous photometric assay is presented which allows to quantify ß-amylase activity and was found to be superior to a conventional test. Finally, the AtDPE2-based maltose assay was used to quantify leaf maltose contents of both Arabidopsis wild type and AtDPE2-deficient plants throughout the light-dark cycle. These data are presented together with assimilatory starch levels.
[Mh] Termos MeSH primário: Arabidopsis/metabolismo
Sistema da Enzima Desramificadora do Glicogênio/metabolismo
Maltose/metabolismo
Fotometria/métodos
Plantas Geneticamente Modificadas/metabolismo
Amido/metabolismo
Sacarose/metabolismo
[Mh] Termos MeSH secundário: Citosol/metabolismo
Ensaios Enzimáticos/métodos
Folhas de Planta/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycogen Debranching Enzyme System); 57-50-1 (Sucrose); 69-79-4 (Maltose); 9005-25-8 (Starch); EC 2.4.1.25 (4 alpha-glucanotransferase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170604
[St] Status:MEDLINE


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[PMID]:28522291
[Au] Autor:Tumhom S; Krusong K; Pongsawasdi P
[Ad] Endereço:Starch and Cyclodextrin Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
[Ti] Título:Y418 in 410s loop is required for high transglucosylation activity and large-ring cyclodextrin production of amylomaltase from Corynebacterium glutamicum.
[So] Source:Biochem Biophys Res Commun;488(3):516-521, 2017 Jul 01.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Amylomaltase catalyzes α-1,4 glucosyl transfer reaction to yield linear or cyclic oligosaccharide products. The aim of this work is to investigate functional roles of 410s loop unique to amylomaltase from Corynebacterium glutamicum (CgAM). Site-directed mutagenesis of Y418, the residue at the loop tip, was performed. Y418A/S/D/R/W/F - CgAMs were characterized and compared to the wild-type (WT). A significant decrease in starch transglucosylation, disproportionation and cyclization activities was observed. Specificity for G3 substrate in disproportionation reaction was not changed; however, Y418F showed an increase in preference for longer oligosaccharides G5 to G7. The catalytic efficiency of Y418 mutated CgAMs, except for Y418F, was significantly lower (up to 8- and 12- fold for the W and R mutants, respectively) than that of WT. The change was in the k , not the K values which were around 16-20 mM. The profile of large-ring cyclodextrin (LR-CD) product was different; the principal product of Y418A/D/S was shifted to the larger size (CD36-CD40) while that of the WT and Y418F peaked at CD29-CD33. The product yield was reduced especially in W and R mutants. Hence Y418 in 410s loop of CgAM not only contributes to transglucosylation activities but also controls the amount and size of LR-CD products through the proposed hydrophobic stacking interaction and the suitable distance of loop channel for substrate entering. This is the first report to show the effect of the loop tip residue on LR-CD product formation.
[Mh] Termos MeSH primário: Corynebacterium glutamicum/enzimologia
Ciclodextrinas/biossíntese
Sistema da Enzima Desramificadora do Glicogênio/metabolismo
[Mh] Termos MeSH secundário: Sistema da Enzima Desramificadora do Glicogênio/genética
Glicosilação
Mutagênese Sítio-Dirigida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclodextrins); 0 (Glycogen Debranching Enzyme System); EC 2.4.1.25 (4 alpha-glucanotransferase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE


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[PMID]:28423489
[Au] Autor:Li L; Lu J; Xue W; Wang L; Zhai Y; Fan Z; Wu G; Fan F; Li J; Zhang C; Zhang Y; Zhao J
[Ad] Endereço:Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
[Ti] Título:Target of obstructive sleep apnea syndrome merge lung cancer: based on big data platform.
[So] Source:Oncotarget;8(13):21567-21578, 2017 Mar 28.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Based on our hospital database, the incidence of lung cancer diagnoses was similar in obstructive sleep apnea Syndrome (OSAS) and hospital general population; among individual with a diagnosis of lung cancer, the presence of OSAS was associated with an increased risk for mortality. In the gene expression and network-level information, we revealed significant alterations of molecules related to HIF1 and metabolic pathways in the hypoxic-conditioned lung cancer cells. We also observed that GBE1 and HK2 are downstream of HIF1 pathway important in hypoxia-conditioned lung cancer cell. Furthermore, we used publicly available datasets to validate that the late-stage lung adenocarcinoma patients showed higher expression HK2 and GBE1 than early-stage ones. In terms of prognostic features, a survival analysis revealed that the high GBE1 and HK2 expression group exhibited poorer survival in lung adenocarcinoma patients. By analyzing and integrating multiple datasets, we identify molecular convergence between hypoxia and lung cancer that reflects their clinical profiles and reveals molecular pathways involved in hypoxic-induced lung cancer progression. In conclusion, we show that OSAS severity appears to increase the risk of lung cancer mortality.
[Mh] Termos MeSH primário: Adenocarcinoma/complicações
Carcinoma Pulmonar de Células não Pequenas/complicações
Neoplasias Pulmonares/complicações
Apneia Obstrutiva do Sono/complicações
[Mh] Termos MeSH secundário: Adenocarcinoma/genética
Adenocarcinoma/mortalidade
Adulto
Idoso
Carcinoma Pulmonar de Células não Pequenas/genética
Carcinoma Pulmonar de Células não Pequenas/mortalidade
Feminino
Perfilação da Expressão Gênica
Sistema da Enzima Desramificadora do Glicogênio/biossíntese
Seres Humanos
Hipóxia/complicações
Hipóxia/genética
Incidência
Estimativa de Kaplan-Meier
Cinesina/biossíntese
Neoplasias Pulmonares/genética
Neoplasias Pulmonares/mortalidade
Masculino
Meia-Idade
Análise de Sequência com Séries de Oligonucleotídeos
Apneia Obstrutiva do Sono/epidemiologia
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycogen Debranching Enzyme System); 0 (KIF2A protein, human); EC 2.4.1.18 (GBE1 protein, human); EC 3.6.4.4 (Kinesin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15372


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[PMID]:28399167
[Au] Autor:Gangoiti J; Lamothe L; van Leeuwen SS; Vafiadi C; Dijkhuizen L
[Ad] Endereço:Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands.
[Ti] Título:Characterization of the Paenibacillus beijingensis DSM 24997 GtfD and its glucan polymer products representing a new glycoside hydrolase 70 subfamily of 4,6-α-glucanotransferase enzymes.
[So] Source:PLoS One;12(4):e0172622, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previously we have reported that the Gram-negative bacterium Azotobacter chroococcum NCIMB 8003 uses the 4,6-α-glucanotransferase GtfD to convert maltodextrins and starch into a reuteran-like polymer consisting of (α1→4) glucan chains connected by alternating (α1→4)/(α1→6) linkages and (α1→4,6) branching points. This enzyme constituted the single evidence for this reaction and product specificity in the GH70 family, mostly containing glucansucrases encoded by lactic acid bacteria (http://www.CAZy.org). In this work, 4 additional GtfD-like proteins were identified in taxonomically diverse plant-associated bacteria forming a new GH70 subfamily with intermediate characteristics between the evolutionary related GH13 and GH70 families. The GtfD enzyme encoded by Paenibacillus beijingensis DSM 24997 was characterized providing the first example of a reuteran-like polymer synthesizing 4,6-α-glucanotransferase in a Gram-positive bacterium. Whereas the A. chroococcum GtfD activity on amylose resulted in the synthesis of a high molecular polymer, in addition to maltose and other small oligosaccharides, two reuteran-like polymer distributions are produced by P. beijingensis GtfD: a high-molecular mass polymer and a low-molecular mass polymer with an average Mw of 27 MDa and 19 kDa, respectively. Compared to the A. chroooccum GtfD product, both P. beijingensis GtfD polymers contain longer linear (α1→4) sequences in their structure reflecting a preference for transfer of even longer glucan chains by this enzyme. Overall, this study provides new insights into the evolutionary history of GH70 enzymes, and enlarges the diversity of natural enzymes that can be applied for modification of the starch present in food into less and/or more slowly digestible carbohydrate structures.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Glucanos/metabolismo
Sistema da Enzima Desramificadora do Glicogênio/metabolismo
Paenibacillus/enzimologia
[Mh] Termos MeSH secundário: Amilose/metabolismo
Animais
Azotobacter/enzimologia
Azotobacter/genética
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Proteínas de Bactérias/isolamento & purificação
Cromatografia
Escherichia coli
Evolução Molecular
Sistema da Enzima Desramificadora do Glicogênio/química
Sistema da Enzima Desramificadora do Glicogênio/genética
Sistema da Enzima Desramificadora do Glicogênio/isolamento & purificação
Seres Humanos
Espectroscopia de Ressonância Magnética
Metilação
Paenibacillus/genética
Filogenia
Domínios Proteicos
Ratos
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Glucans); 0 (Glycogen Debranching Enzyme System); 0 (Recombinant Proteins); 3BD5BBR49M (maltoheptaose); 9005-82-7 (Amylose); EC 2.4.1.25 (4 alpha-glucanotransferase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172622


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[PMID]:28355295
[Au] Autor:Hong EP; Go MJ; Kim HL; Park JW
[Ad] Endereço:Department of Medical Genetics, College of Medicine, Hallym University, Chuncheon-si, Ganwon-do, Republic of Korea.
[Ti] Título:Risk prediction of pulmonary tuberculosis using genetic and conventional risk factors in adult Korean population.
[So] Source:PLoS One;12(3):e0174642, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A complex interplay among host, pathogen, and environmental factors is believed to contribute to the risk of developing pulmonary tuberculosis (PTB). The lack of replication of published genome-wide association study (GWAS) findings limits the clinical utility of reported single nucleotide polymorphisms (SNPs). We conducted a GWAS using 467 PTB cases and 1,313 healthy controls obtained from two community-based cohorts in Korea. We evaluated the performance of PTB risk models based on different combinations of genetic and nongenetic factors and validated the results in an independent Korean population comprised of 179 PTB cases and 500 healthy controls. We demonstrated the polygenic nature of PTB and nongenetic factors such as age, sex, and body mass index (BMI) were strongly associated with PTB risk. None of the SNPs achieved genome-wide significance; instead, we were able to replicate the associations between PTB and ten SNPs near or in the genes, CDCA7, GBE1, GADL1, SPATA16, C6orf118, KIAA1432, DMRT2, CTR9, CCDC67, and CDH13, which may play roles in the immune and inflammatory pathways. Among the replicated SNPs, an intergenic SNP, rs9365798, located downstream of the C6orf118 gene showed the most significant association under the dominant model (OR = 1.59, 95% CI 1.32-1.92, P = 2.1×10-6). The performance of a risk model combining the effects of ten replicated SNPs and six nongenetic factors (i.e., age, sex, BMI, cigarette smoking, systolic blood pressure, and hemoglobin) were validated in the replication set (AUC = 0.80, 95% CI 0.76-0.84). The strategy of combining genetic and nongenetic risk factors ultimately resulted in better risk prediction for PTB in the adult Korean population.
[Mh] Termos MeSH primário: Predisposição Genética para Doença/genética
Estudo de Associação Genômica Ampla/métodos
Polimorfismo de Nucleotídeo Único
Tuberculose Pulmonar/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Consumo de Bebidas Alcoólicas
Grupo com Ancestrais do Continente Asiático/genética
Pressão Sanguínea
Índice de Massa Corporal
Estudos de Coortes
Feminino
Predisposição Genética para Doença/etnologia
Sistema da Enzima Desramificadora do Glicogênio/genética
Seres Humanos
Modelos Logísticos
Masculino
Meia-Idade
Proteínas Nucleares/genética
República da Coreia
Medição de Risco/métodos
Medição de Risco/estatística & dados numéricos
Fatores de Risco
Fumar
Tuberculose Pulmonar/etnologia
Tuberculose Pulmonar/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CDCA7 protein, human); 0 (Glycogen Debranching Enzyme System); 0 (Nuclear Proteins); EC 2.4.1.18 (GBE1 protein, human)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174642


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[PMID]:28140467
[Au] Autor:Madsen LR; Stanley S; Swann P; Oswald J
[Ad] Endereço:Authors are with ISOThrive, LLC, 9385 Discovery Blvd. Suite 133, Manassas, VA, 20109, U.S.A.
[Ti] Título:A Survey of Commercially Available Isomaltooligosaccharide-Based Food Ingredients.
[So] Source:J Food Sci;82(2):401-408, 2017 Feb.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Isomaltooligosaccharides (IMOs) are included in many commercially available food products including protein/fiber bars, shakes, and other dietary supplements. Marketed as "high fiber," "prebiotic soluble fiber," and/or as a "low-calorie, low glycemic sweetener," IMO may be present in significant amounts, for example, more than 15 g/item or serving. Herein, high-pressure anion exchange chromatography with pulsed amperometric detection and high-pressure liquid chromatography with differential refractive index detection are used to compare 7 commercially available IMO-containing bulk food ingredients. The ingredients are typical of those produced either (a) via bacterial fermentation ("fermented" IMO or MIMO) of sucrose in the presence of a maltose acceptor mediated by a glucosyltransferase enzyme (dextransucrase), or (b) via transglycosylation of hydrolyzed starch with α-glucosidase ("industrial" IMO). Analysis of the results with respect to digestibility suggests that the potential glycemic impact of the ingredients and products containing "industrial" IMO may be inconsistent with the product labeling and/or certificates of analysis with respect to overall fiber content, prebiotic fiber content, and glycemic response and are thus inappropriate for diabetic patients and those on low-carbohydrate (for example, ketogenic) diets.
[Mh] Termos MeSH primário: Análise de Alimentos
Isomaltose/análise
Oligossacarídeos/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Fibras na Dieta/análise
Análise de Alimentos/economia
Sistema da Enzima Desramificadora do Glicogênio/química
Prebióticos/análise
Inquéritos e Questionários
alfa-Glucosidases/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fiber); 0 (Glycogen Debranching Enzyme System); 0 (Oligosaccharides); 0 (Prebiotics); 67I334IX2M (Isomaltose); EC 2.4.1.25 (4 alpha-glucanotransferase); EC 3.2.1.20 (alpha-Glucosidases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13623


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[PMID]:28065507
[Au] Autor:Bai Y; Gangoiti J; Dijkstra BW; Dijkhuizen L; Pijning T
[Ad] Endereço:Laboratory of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, the Netherlands.
[Ti] Título:Crystal Structure of 4,6-α-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from α-Amylases in Oral Bacteria.
[So] Source:Structure;25(2):231-242, 2017 Feb 07.
[Is] ISSN:1878-4186
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Food processing and refining has dramatically changed the human diet, but little is known about whether this affected the evolution of enzymes in human microbiota. We present evidence that glycoside hydrolase family 70 (GH70) glucansucrases from lactobacilli, synthesizing α-glucan-type extracellular polysaccharides from sucrose, likely evolved from GH13 starch-acting α-amylases, via GH70 4,6-α-glucanotransferases. The crystal structure of a 4,6-α-glucanotransferase explains the mode of action and unique product specificity of these enzymes. While the α-amylase substrate-binding scaffold is retained, active-site loops adapted to favor transglycosylation over hydrolysis; the structure also gives clues as to how 4,6-α-glucanotransferases may have evolved further toward sucrose utilization instead of starch. Further supported by genomic, phylogenetic, and in vivo studies, we propose that dietary changes involving starch (and starch derivatives) and sucrose intake were critical factors during the evolution of 4,6-α-GTs and glucansucrases from α-amylases, allowing oral bacteria to produce extracellular polymers that contribute to biofilm formation from different substrates.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Sistema da Enzima Desramificadora do Glicogênio/química
Lactobacillus reuteri/genética
Amido/química
Sacarose/química
alfa-Amilases/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Sequência de Carboidratos
Domínio Catalítico
Clonagem Molecular
Cristalografia por Raios X
Dieta
Carboidratos da Dieta/administração & dosagem
Evolução Molecular
Microbioma Gastrointestinal/genética
Expressão Gênica
Sistema da Enzima Desramificadora do Glicogênio/genética
Sistema da Enzima Desramificadora do Glicogênio/metabolismo
Glicosilação
Seres Humanos
Lactobacillus reuteri/classificação
Lactobacillus reuteri/enzimologia
Modelos Moleculares
Mutação
Filogenia
Ligação Proteica
Conformação Proteica em alfa-Hélice
Conformação Proteica em Folha beta
Domínios e Motivos de Interação entre Proteínas
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Amido/metabolismo
Especificidade por Substrato
Sacarose/metabolismo
alfa-Amilases/genética
alfa-Amilases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Dietary Carbohydrates); 0 (Glycogen Debranching Enzyme System); 0 (Recombinant Proteins); 57-50-1 (Sucrose); 9005-25-8 (Starch); EC 2.4.1.25 (4 alpha-glucanotransferase); EC 3.2.1.1 (alpha-Amylases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170110
[St] Status:MEDLINE


  9 / 400 MEDLINE  
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[PMID]:27911907
[Au] Autor:Xu X; Dechesne A; Visser RG; Trindade LM
[Ad] Endereço:Wageningen UR Plant Breeding, Wageningen University and Research, P.O. Box 386, 6700, AJ, Wageningen. The Netherlands.
[Ti] Título:Expression of an (Engineered) 4,6-α-Glucanotransferase in Potato Results in Changes in Starch Characteristics.
[So] Source:PLoS One;11(12):e0166981, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Starch structure strongly influences starch physicochemical properties, determining the end uses of starch in various applications. To produce starches with novel structure and exploit the mechanism of starch granule formation, an (engineered) 4, 6-α-glucanotransferase (GTFB) from Lactobacillus reuteri 121 was introduced into two potato genetic backgrounds: amylose-containing line Kardal and amylose-free mutant amf. The resulting starches showed severe changes in granule morphology regardless of genetic backgrounds. Modified starches from amf background exhibited a significant increase in granule size and starch phosphate content relative to the control, while starches from Kardal background displayed a higher digestibility, but did not show changes in granule size and phosphate content. Transcriptome analysis revealed the existence of a mechanism to restore the regular packing of double helices in starch granules, which possibly resulted in the removal of novel glucose chains potentially introduced by the (engineered) GTFB. This amendment mechanics would also explain the difficulties to detect alterations in starch fine structure in the transgenic lines.
[Mh] Termos MeSH primário: Proteínas de Bactérias/biossíntese
Expressão Gênica
Sistema da Enzima Desramificadora do Glicogênio/biossíntese
Lactobacillus reuteri/genética
Plantas Geneticamente Modificadas
Solanum tuberosum
Amido
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Sistema da Enzima Desramificadora do Glicogênio/genética
Lactobacillus reuteri/enzimologia
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/metabolismo
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/genética
Solanum tuberosum/genética
Solanum tuberosum/metabolismo
Amido/genética
Amido/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Glycogen Debranching Enzyme System); 0 (Recombinant Proteins); 9005-25-8 (Starch); EC 2.4.1.25 (4 alpha-glucanotransferase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161203
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166981


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[PMID]:27595989
[Au] Autor:Oldenburg D; Ru Y; Weinhaus B; Cash S; Theodorescu D; Guin S
[Ad] Endereço:Gundersen Medical Foundation, 1300 Badger Street, La Crosse, WI, 54601, USA.
[Ti] Título:CD44 and RHAMM are essential for rapid growth of bladder cancer driven by loss of Glycogen Debranching Enzyme (AGL).
[So] Source:BMC Cancer;16:713, 2016 09 05.
[Is] ISSN:1471-2407
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Loss of Amylo-alpha-1-6-glucosidase-4-alpha-glucanotransferase (AGL) drives rapid proliferation of bladder cancer cells by upregulating Hyaluronic acid(HA) Synthase (HAS2) mediated HA synthesis. However the role of HA receptors CD44 and Hyaluronan Mediated Motility Receptor (RHAMM) in regulating the growth of bladder cancer cells driven by loss of AGL has not been studied. METHODS: Western blot analysis and Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay was carried out to study cellular apoptosis with HAS2, CD44 and RHAMM loss in bladder cancer cells with and without AGL expression. Proliferation and softagar assays were carried out to study cellular anchorage dependent and independent growth. Clinicopathologic analysis was carried out on bladder cancer patient datasets. RESULTS: Higher amounts of cleaved Cas3, Cas9 and PARP was observed in AGL low bladder cancer cell with loss of HAS2, CD44 or RHAMM. TUNEL staining showed more apoptotic cells with loss of HAS2, CD44 or RHAMM in AGL low bladder cancer cells. This revealed that bladder cancer cells whose aggressive growth is mediated by loss of AGL are susceptible to apoptosis with loss of HAS2, CD44 or RHAMM. Interestingly loss of either CD44 or RHAMM induces apoptosis in different low AGL expressing bladder cancer cell lines. Growth assays showed that loss of CD44 and RHAMM predominantly inhibit anchorage dependent and independent growth of AGL low bladder cancer cells. Clinicopathologic analysis revealed that high RHAMM mRNA expression is a marker of poor patient outcome in bladder cancer and patients with high RHAMM and low AGL tumor mRNA expression have poor survival. CONCLUSION: Our findings strongly point to the importance of the HAS2-HA-CD44/RHAMM pathway for rapid growth of bladder cancer cells with loss of AGL and provides rational for targeting this pathway at various steps for "personalized" treatment of bladder cancer patients based of their AGL expression status.
[Mh] Termos MeSH primário: Proteínas da Matriz Extracelular/metabolismo
Sistema da Enzima Desramificadora do Glicogênio/metabolismo
Receptores de Hialuronatos/metabolismo
Neoplasias da Bexiga Urinária/patologia
[Mh] Termos MeSH secundário: Western Blotting
Linhagem Celular Tumoral
Ensaio de Imunoadsorção Enzimática
Imunofluorescência
Glucuronosiltransferase/metabolismo
Seres Humanos
Hialuronan Sintases
Marcação In Situ das Extremidades Cortadas
Reação em Cadeia da Polimerase
Neoplasias da Bexiga Urinária/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CD44 protein, human); 0 (Extracellular Matrix Proteins); 0 (Glycogen Debranching Enzyme System); 0 (Hyaluronan Receptors); 0 (hyaluronan-mediated motility receptor); EC 2.4.1.17 (Glucuronosyltransferase); EC 2.4.1.212 (HAS2 protein, human); EC 2.4.1.212 (Hyaluronan Synthases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160907
[St] Status:MEDLINE
[do] DOI:10.1186/s12885-016-2756-5



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