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[PMID]:28167125
[Au] Autor:Yan M; Xu L; Jiang H; Zhou Z; Zhou S; Zhang L
[Ad] Endereço:Department of Haematology, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou 510623, PR China. Electronic address: robinbobo@qq.com.
[Ti] Título:PMA-LAMP for rapid detection of Escherichia coli and shiga toxins from viable but non-culturable state.
[So] Source:Microb Pathog;105:245-250, 2017 Apr.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In exposure to outer pressure, microorganisms are capable of entry into the Viable But Non-Culturable (VBNC) state, and thus survive under various elimination processing. The survival microorganisms may yield negative results on culturing, and cause false negative for this golden standard methodology. In this study, a novel PMA-LAMP assay on the detection of Enterohemorrhage E. coli and shiga toxins has been developed and evaluated, with further application on a number of food borne E. coli strains. LAMP primers were designed on the target of rfbe for Enterohemorrhage E. coli and stx1with stx2 for shiga toxins. Via specific penetration through the damaged cell membrane of dead cells and intercalating into DNA, PMA could prevent DNA amplification of dead bacteria from LAMP, which enabled the differentiation of bacteria between VBNC state and dead state. The established PMA-LAMP showed significant advantage in rapidity, sensitivity and specificity, compared with regular PCR assay. The applicability had also been verified, demonstrating the PMA-LAMP was capable of detection on Enterohemorrhage E. coli and shiga toxins.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas/métodos
Escherichia coli/isolamento & purificação
Microbiologia de Alimentos/métodos
Toxinas Shiga/isolamento & purificação
[Mh] Termos MeSH secundário: Carboidratos Epimerases/genética
Primers do DNA
DNA Bacteriano/genética
Escherichia coli/genética
Escherichia coli O157/genética
Escherichia coli O157/isolamento & purificação
Inocuidade dos Alimentos/métodos
Doenças Transmitidas por Alimentos/microbiologia
Testes de Sensibilidade Microbiana
Viabilidade Microbiana
Técnicas de Amplificação de Ácido Nucleico/métodos
Reação em Cadeia da Polimerase
Toxina Shiga I/genética
Toxina Shiga II/genética
Toxinas Shiga/genética
Transaminases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Bacterial); 0 (Shiga Toxin 1); 0 (Shiga Toxin 2); 0 (Shiga Toxins); EC 2.6.1.- (Transaminases); EC 5.1.3.- (Carbohydrate Epimerases); EC 5.1.3.- (perosamine synthetase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE


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[PMID]:28165371
[Au] Autor:Kavaliauskiene S; Dyve Lingelem AB; Skotland T; Sandvig K
[Ad] Endereço:Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, N-0379 Oslo, Norway. simona.kavaliauskiene@rr-research.no.
[Ti] Título:Protection against Shiga Toxins.
[So] Source:Toxins (Basel);9(2), 2017 Feb 03.
[Is] ISSN:2072-6651
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Shiga toxins consist of an A-moiety and five B-moieties able to bind the neutral glycosphingolipid globotriaosylceramide (Gb3) on the cell surface. To intoxicate cells efficiently, the toxin A-moiety has to be cleaved by furin and transported retrogradely to the Golgi apparatus and to the endoplasmic reticulum. The enzymatically active part of the A-moiety is then translocated to the cytosol, where it inhibits protein synthesis and in some cell types induces apoptosis. Protection of cells can be provided either by inhibiting binding of the toxin to cells or by interfering with any of the subsequent steps required for its toxic effect. In this article we provide a brief overview of the interaction of Shiga toxins with cells, describe some compounds and conditions found to protect cells against Shiga toxins, and discuss whether they might also provide protection in animals and humans.
[Mh] Termos MeSH primário: Antídotos/farmacologia
Proteínas de Bactérias/antagonistas & inibidores
Disenteria Bacilar/prevenção & controle
Síndrome Hemolítico-Urêmica/prevenção & controle
Toxinas Shiga/antagonistas & inibidores
Escherichia coli Shiga Toxigênica/efeitos dos fármacos
Shigella dysenteriae/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Apoptose
Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Disenteria Bacilar/metabolismo
Disenteria Bacilar/microbiologia
Síndrome Hemolítico-Urêmica/metabolismo
Síndrome Hemolítico-Urêmica/microbiologia
Interações Hospedeiro-Patógeno
Seres Humanos
Biossíntese de Proteínas
Conformação Proteica
Transporte Proteico
Toxinas Shiga/química
Toxinas Shiga/metabolismo
Escherichia coli Shiga Toxigênica/metabolismo
Escherichia coli Shiga Toxigênica/patogenicidade
Shigella dysenteriae/metabolismo
Shigella dysenteriae/patogenicidade
Relação Estrutura-Atividade
Triexosilceramidas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antidotes); 0 (Bacterial Proteins); 0 (Shiga Toxins); 0 (Trihexosylceramides); 71965-57-6 (globotriaosylceramide)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170207
[St] Status:MEDLINE


  3 / 1347 MEDLINE  
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[PMID]:28127846
[Au] Autor:Jones M; Octavia S; Lammers G; Heller J; Lan R
[Ad] Endereço:School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
[Ti] Título:Population and evolutionary dynamics of Shiga-toxin producing Escherichia coli O157 in a beef herd: A longitudinal study.
[So] Source:Environ Microbiol;19(5):1836-1844, 2017 May.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Shiga toxin producing Escherichia coli O157:H7 (STEC O157) is naturally found in the gastrointestinal tract of cattle and can cause severe disease in humans. There is limited understanding of the population dynamics and microevolution of STEC O157 at herd level. In this study, isolates from a closed beef herd of 23 cows were used to examine the population turnover in the herd. Of the nine STEC O157 clades previously described, clade 7 was found in 162 of the 169 isolates typed. Multiple locus variable number tandem repeat analysis (MLVA) differentiated 169 isolates into 33 unique MLVA types. Five predominant MLVA types were evident with most of the remaining types containing only a single isolate. MLVA data suggest that over time clonal replacement occurred within the herd. Genome sequencing of 18 selected isolates found that the isolates were divided into four lineages, representing four different 'clones' in the herd. Genome data confirmed clonal replacement over time and provided evidence of cross transmission of strains between cows. The findings enhanced our understanding of the population dynamics of STEC O157 in its natural host that will help developing effective control measures to prevent the spread of the pathogen to the human population.
[Mh] Termos MeSH primário: Escherichia coli O157/genética
Escherichia coli O157/metabolismo
Microbioma Gastrointestinal/genética
Genoma Bacteriano/genética
Repetições Minissatélites/genética
Tipagem Molecular/métodos
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Bovinos
Infecções por Escherichia coli/epidemiologia
Infecções por Escherichia coli/microbiologia
Escherichia coli O157/isolamento & purificação
Feminino
Seres Humanos
Estudos Longitudinais
Filogenia
Polimorfismo de Nucleotídeo Único/genética
Dinâmica Populacional
Carne Vermelha/microbiologia
Toxinas Shiga/genética
Toxinas Shiga/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Shiga Toxins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13679


  4 / 1347 MEDLINE  
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[PMID]:28056274
[Au] Autor:Shao CC; Hu B; Bi ZW; Kou ZQ; Fang M; Chen BL; Bi ZQ
[Ad] Endereço:Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan 250012, China.
[Ti] Título:[Serotype identification and antibiotic susceptibility of Shiga toxin-producing Escherichia coli in the Weishan area in Shandong Province, China].
[So] Source:Zhonghua Yu Fang Yi Xue Za Zhi;51(1):70-75, 2017 Jan 06.
[Is] ISSN:0253-9624
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To determine the serotypes and drug resistance profiles of Shiga toxin-producing (STEC) in animal stools from the Weishan area in Shandong Province, China. To provide the basis for further study. Five hundred animal stool samples (from pigs, cattle, sheep, dogs and birds) were collected from the Weishan area and STEC strains were isolated from these samples. Strains were serotyped by a serum agglutination test, and their drug resistance profiles were determined through antimicrobial sensitivity experiments. In this study, PCR was used to detect tetracycline resistance genes ( , , , ) and beta-lactam resistance genes ( -1, - , ). Sixteen strains of STEC were isolated from animal stool samples. Thirteen strains were isolated from pig stool samples, two from bovine stool samples and one from a sheep stool sample. Two of the strains were identified as O157:H7, and other 14 strains were non-O157 STEC of different serotypes. Antimicrobial sensitivity experiments showed that 15 of the strains were multidrug resistant. The rates of resistance were as follows: nalidixic acid (12/16 strains), sulfisoxazole (11/16), trimethoprim and sulphame-thoxazole (11/16), doxycycline (9/16), azithromycin (9/16), tetracycline (9/16), chloramphenicol (8/16) and streptomycin (8/16). Therefore, nalidixic acid showed the highest rate of resistance among the strains, followed by trimethoprim and sulphame-thoxazole, and sulfisoxazole. Resistance to cefepime or imipenem was not detected. In total, three types of drug resistance genes ( , and ) were detected among the 16 strains. The results showed that STEC strains isolated from animals in the Weishan area were of a range of serotypes. The 16 strains of STEC isolated from animal stools in this area were resistant to a number of antibiotics, with many strains displaying multidrug resistance.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana Múltipla/genética
Infecções por Escherichia coli/veterinária
Fezes/microbiologia
Toxinas Shiga/genética
Escherichia coli Shiga Toxigênica/efeitos dos fármacos
Escherichia coli Shiga Toxigênica/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Bovinos
China
Cães
Escherichia coli
Infecções por Escherichia coli/microbiologia
Escherichia coli O157/efeitos dos fármacos
Escherichia coli O157/genética
Escherichia coli O157/isolamento & purificação
Proteínas de Escherichia coli/metabolismo
Testes de Sensibilidade Microbiana
Fenótipo
Reação em Cadeia da Polimerase
Sorogrupo
Ovinos
Toxinas Shiga/classificação
Escherichia coli Shiga Toxigênica/classificação
Escherichia coli Shiga Toxigênica/genética
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Escherichia coli Proteins); 0 (Shiga Toxins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0253-9624.2017.01.014


  5 / 1347 MEDLINE  
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[PMID]:27987365
[Au] Autor:Baranzoni GM; Fratamico PM; Boccia F; Bagi LK; Kim GH; Anastasio A; Pepe T
[Ad] Endereço:U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA, USA.
[Ti] Título:Evaluation of the performance of the IQ-Check kits and the USDA Microbiology Laboratory Guidebook methods for detection of Shiga toxin-producing Escherichia coli (STEC) and STEC and Salmonella simultaneously in ground beef.
[So] Source:J Appl Microbiol;122(3):809-816, 2017 Mar.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: To evaluate the performance of the IQ-Check kits and the USDA Microbiology Laboratory Guidebook (MLG) methods for detection of the top seven Shiga toxin-producing Escherichia coli (STEC) (O157:H7, O26, O45, O103, O111, O121 and O145) in ground beef and both STEC and Salmonella in co-inoculated samples. METHODS AND RESULTS: Ground beef samples inoculated with ~10 CFU of STEC or both STEC and Salmonella Typhimurium were stored at 4°C for 72 h, followed by screening with the IQ-Check and BAX System kit (MLG) methods that employ different enrichment media. STEC and S. Typhimurium were detected after 12 and 18 h and their presence was confirmed by colony isolation. CONCLUSIONS: Both methods were able to detect STEC in ground beef after 12 h of enrichment in samples inoculated with low levels of the pathogen. STEC and S. Typhimurium can be detected and isolated in co-inoculated ground beef samples. SIGNIFICANCE AND IMPACT OF THE STUDY: The IQ-Check methods are comparable to the MLG methods for detection of STEC and simultaneous detection of STEC and S. Typhimurium in seeded ground beef after a short enrichment time, thus the IQ-Check method can be useful for the food industry for rapid detection of these pathogens.
[Mh] Termos MeSH primário: Reação em Cadeia da Polimerase/métodos
Carne Vermelha/microbiologia
Salmonella/isolamento & purificação
Escherichia coli Shiga Toxigênica/isolamento & purificação
United States Department of Agriculture
[Mh] Termos MeSH secundário: Animais
Bovinos
Microbiologia de Alimentos
Guias como Assunto
Reação em Cadeia da Polimerase/normas
Salmonella/genética
Toxinas Shiga/análise
Toxinas Shiga/genética
Escherichia coli Shiga Toxigênica/genética
Estados Unidos
Fatores de Virulência/genética
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Shiga Toxins); 0 (Virulence Factors)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161218
[St] Status:MEDLINE
[do] DOI:10.1111/jam.13380


  6 / 1347 MEDLINE  
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[PMID]:27914959
[Au] Autor:González J; Sanso AM; Cadona JS; Bustamante AV
[Ad] Endereço:Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, Argentina.
[Ti] Título:Virulence traits and different nle profiles in cattle and human verotoxin-producing Escherichia coli O157:H7 strains from Argentina.
[So] Source:Microb Pathog;102:102-108, 2017 Jan.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Verotoxin-producing E. coli (VTEC) O157:H7 is the dominant serotype isolated from patients with HUS and, Argentina has the highest rate of HUS in the world. Molecular typing had allowed to identify subpopulations related to the origin and virulence of O157:H7 strains. Our aim was to perform a genetic characterization of 43 O157:H7 strains isolated in Argentine mostly from cattle and humans in order to establish the potential public health risk. For it, we used a combination of molecular subtyping methods in order to identify clade 8_rhsA (C3468G), LSPA-6 and virulence profiles and, a cytotoxicity assay on Vero cell. All isolates carried the clade 8 SNP variant and 98% of them belonged to lineage I/II (2% lineage II). Isolates were grouped into eleven nle profiles, 46% were positive for all nle genes, while the remaining isolates, except two, showed incomplete OI-71, particularly lacked nleF. All isolates showed the plasmid profile ehxA-espP-katP-stcE and harbored ehaA, elfA, iha and lpfA variants lpfA1-3 and lpfA2-2 and, ECSP_0242. The frequencies of the remaining ECSP genes were 95% ECSP_2687, 88% ECSP_3286, 86% ECSP_3620, 53% ECSP_2870/2872 and 44% ECSP_1733. All O157:H7 strains, except the isolate identified as lineage II, were cytotoxic on Vero cells. Among Argentinean strains, most genetic markers occur at equal relative frequencies among clinical and bovine isolates, showing diversity mostly in nle genes profiles. The belonging of the isolates to hypervirulent clade 8 and lineage I/II, the high prevalence of nle and putative virulence factors genes, would allow assigning most O157:H7 strains of this region a high risk to public health.
[Mh] Termos MeSH primário: Doenças dos Bovinos/microbiologia
Escherichia coli O157/fisiologia
Proteínas de Escherichia coli/genética
Síndrome Hemolítico-Urêmica/microbiologia
Toxinas Shiga/biossíntese
[Mh] Termos MeSH secundário: Animais
Argentina/epidemiologia
Aderência Bacteriana/genética
Bovinos
Doenças dos Bovinos/epidemiologia
Análise por Conglomerados
Escherichia coli O157/classificação
Genótipo
Síndrome Hemolítico-Urêmica/epidemiologia
Seres Humanos
Fenótipo
Plasmídeos/genética
Virulência/genética
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 0 (Shiga Toxins); 0 (Virulence Factors)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161205
[St] Status:MEDLINE


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[PMID]:27854514
[Au] Autor:Tostes R; Goji N; Amoako K; Chui L; Kastelic J; DeVinney R; Stanford K; Reuter T
[Ad] Endereço:1 Department of Microbiology, Immunology and Infectious Diseases, University of Calgary , Calgary, Canada .
[Ti] Título:Subtyping Escherichia coli Virulence Genes Isolated from Feces of Beef Cattle and Clinical Cases in Alberta.
[So] Source:Foodborne Pathog Dis;14(1):35-42, 2017 Jan.
[Is] ISSN:1556-7125
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Clinical outcomes of Shiga toxin (stx)-producing Escherichia coli infection are largely determined by virulence gene subtypes. This study used a polymerase chain reaction (PCR)-pyrosequencing assay to analyze single-nucleotide polymorphisms for subtyping three major virulence genes (stx , stx , eae) of pathogenic E. coli (O157, O26, O111, and O103) isolated from cattle over a 2-year interval (n = 465) and human clinical cases (n = 42) in western Canada. Most bovine isolates were PCR positive for at least one target virulence gene (367/465), whereas 100% of human isolates harbored eae in combination with at least one stx gene. Four Shiga toxin (1a, 2a, 2c, and 2e) and four eae (λ/γ1-eae, É›-eae, θ/γ2-eae, and ß-eae) subtypes were identified in over 25 distinct virulence genotypes. Among cattle isolates, every serogroup, but O103, presented a dominant genotype (O157: stx +stx +λ/γ1-eae, O26: ß-eae alone, and O111: stx +θ/γ2-eae). Similar patterns were found in human isolates, although it was not possible to establish a clear genotypic association between the two sources. Many O157 and non-O157 cattle isolates lacked stx genes; the absence was greater in non-O157 (75/258) and O157:non-H7 (19/40) than in O157:H7 strains (1/164). In addition, there was a greater diversity of virulence genotypes of E. coli isolated from cattle than those of human diseases, which could be due to sample characteristics (e.g., source and clinical condition). However, the majority of cattle strains had virulence profiles identical to those of clinical cases. Consequently, determining the presence of certain stx (stx and stx ) and eae (λ/γ1-eae) subtypes known to cause human disease would be a valuable tool for risk assessment and prediction of disease outcome along the farm-to-fork continuum.
[Mh] Termos MeSH primário: Escherichia coli O157/genética
Fezes/microbiologia
Genes Bacterianos
Carne Vermelha/microbiologia
Escherichia coli Shiga Toxigênica/genética
[Mh] Termos MeSH secundário: Alberta
Animais
Carboidratos Epimerases/genética
Bovinos/microbiologia
Escherichia coli O157/isolamento & purificação
Contaminação de Alimentos
Microbiologia de Alimentos
Seres Humanos
Polimorfismo de Nucleotídeo Único
Sorotipagem
Toxinas Shiga/genética
Escherichia coli Shiga Toxigênica/isolamento & purificação
Transaminases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Shiga Toxins); EC 2.6.1.- (Transaminases); EC 5.1.3.- (Carbohydrate Epimerases); EC 5.1.3.- (perosamine synthetase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161118
[St] Status:MEDLINE
[do] DOI:10.1089/fpd.2016.2199


  8 / 1347 MEDLINE  
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[PMID]:27712998
[Au] Autor:Gupta N; Noël R; Goudet A; Hinsinger K; Michau A; Pons V; Abdelkafi H; Secher T; Shima A; Shtanko O; Sakurai Y; Cojean S; Pomel S; Liévin-Le Moal V; Leignel V; Herweg JA; Fischer A; Johannes L; Harrison K; Beard PM; Clayette P; Le Grand R; Rayner JO; Rudel T; Vacus J; Loiseau PM; Davey RA; Oswald E; Cintrat JC; Barbier J; Gillet D
[Ad] Endereço:Institute of Biology and Technology of Saclay (IBITECS), CEA, LabEx LERMIT, Université Paris-Saclay, F-91191, Gif Sur Yvette, France.
[Ti] Título:Inhibitors of retrograde trafficking active against ricin and Shiga toxins also protect cells from several viruses, Leishmania and Chlamydiales.
[So] Source:Chem Biol Interact;267:96-103, 2017 Apr 01.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Medical countermeasures to treat biothreat agent infections require broad-spectrum therapeutics that do not induce agent resistance. A cell-based high-throughput screen (HTS) against ricin toxin combined with hit optimization allowed selection of a family of compounds that meet these requirements. The hit compound Retro-2 and its derivatives have been demonstrated to be safe in vivo in mice even at high doses. Moreover, Retro-2 is an inhibitor of retrograde transport that affects syntaxin-5-dependent toxins and pathogens. As a consequence, it has a broad-spectrum activity that has been demonstrated both in vitro and in vivo against ricin, Shiga toxin-producing O104:H4 entero-hemorrhagic E. coli and Leishmania sp. and in vitro against Ebola, Marburg and poxviruses and Chlamydiales. An effect is anticipated on other toxins or pathogens that use retrograde trafficking and syntaxin-5. Since Retro-2 targets cell components of the host and not directly the pathogen, no selection of resistant pathogens is expected. These lead compounds need now to be developed as drugs for human use.
[Mh] Termos MeSH primário: Benzamidas/farmacologia
Chlamydiales/metabolismo
Ebolavirus/metabolismo
Leishmania/metabolismo
Ricina/metabolismo
Toxinas Shiga/metabolismo
Tiofenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Benzamidas/química
Peso Corporal/efeitos dos fármacos
Chlamydiales/efeitos dos fármacos
Ebolavirus/efeitos dos fármacos
Escherichia coli/metabolismo
Células HEK293
Células HeLa
Seres Humanos
Injeções Intraperitoneais
Leishmania/efeitos dos fármacos
Camundongos
Camundongos Endogâmicos BALB C
Mitomicina/farmacologia
Modelos Animais
Células RAW 264.7
Ricina/antagonistas & inibidores
Toxinas Shiga/antagonistas & inibidores
Tiofenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-(((5-methyl-2-thienyl)methylene)amino)-N-phenylbenzamide); 0 (Benzamides); 0 (Shiga Toxins); 0 (Thiophenes); 50SG953SK6 (Mitomycin); 9009-86-3 (Ricin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161008
[St] Status:MEDLINE


  9 / 1347 MEDLINE  
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[PMID]:28221838
[Au] Autor:Lacher DW; Gangiredla J; Patel I; Elkins CA; Feng PC
[Ad] Endereço:U.S. Food and Drug Administration, Division of Molecular Biology, Laurel, Maryland 20708.
[Ti] Título:Use of the Escherichia coli Identification Microarray for Characterizing the Health Risks of Shiga Toxin-Producing Escherichia coli Isolated from Foods.
[So] Source:J Food Prot;79(10):1656-1662, 2016 Oct.
[Is] ISSN:1944-9097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:More than 470 serotypes of Shiga toxin-producing Escherichia coli (STEC) have been identified, but not all cause severe illness in humans. Most STEC that cause severe diseases can adhere to epithelial cells, produce specific stx subtypes, and belong to certain serotypes; therefore, these traits appear to be critical STEC risk factors. However, testing for these traits is labor intensive, and serotyping is inadequate because of extensive variations among E. coli O and H antigen types. In the present study, the E. coli identification microarray, which tests for over 40,000 E. coli gene targets, was examined for its potential to quickly characterize STEC strains. Analysis of 47 E. coli isolates, including 31 STEC isolates, recovered from 39 foods revealed that the microarray effectively determined the presence or absence of adherence genes and identified the specific eae allele in 3 isolates. The array identified most of the stx subtypes carried by all the isolates but had some difficulties in discerning between stx , stx , and stx because of the genetic similarities of these subtypes. The array determined the O and H types of 68 and 96% of the isolates, respectively, and although most serotypes were unremarkable, a few known pathogenic serotypes were also found. These selected STEC traits provided a scientific basis for assessing the potential health risks of STEC strains and also showed the importance of H typing in determining health risks. However, the diversity of the STEC group, the complexity of virulence mechanisms, and the variation in pathotypes among strains continue to pose challenges to assessing the potential of STEC strains to cause severe illness.
[Mh] Termos MeSH primário: Escherichia coli/isolamento & purificação
Escherichia coli Shiga Toxigênica/isolamento & purificação
[Mh] Termos MeSH secundário: Infecções por Escherichia coli
Proteínas de Escherichia coli/genética
Microbiologia de Alimentos
Seres Humanos
Sorotipagem
Toxinas Shiga
Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 0 (Shiga Toxins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-16-176


  10 / 1347 MEDLINE  
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[PMID]:27935997
[Au] Autor:Adnan H; Zhang Z; Park HJ; Tailor C; Che C; Kamani M; Spitalny G; Binnington B; Lingwood C
[Ad] Endereço:Division of Molecular Structure and Function, The Hospital for Sick Children, Toronto, Ontario, Canada.
[Ti] Título:Endoplasmic Reticulum-Targeted Subunit Toxins Provide a New Approach to Rescue Misfolded Mutant Proteins and Revert Cell Models of Genetic Diseases.
[So] Source:PLoS One;11(12):e0166948, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many germ line diseases stem from a relatively minor disturbance in mutant protein endoplasmic reticulum (ER) 3D assembly. Chaperones are recruited which, on failure to correct folding, sort the mutant for retrotranslocation and cytosolic proteasomal degradation (ER-associated degradation-ERAD), to initiate/exacerbate deficiency-disease symptoms. Several bacterial (and plant) subunit toxins, retrograde transport to the ER after initial cell surface receptor binding/internalization. The A subunit has evolved to mimic a misfolded protein and hijack the ERAD membrane translocon (dislocon), to effect cytosolic access and cytopathology. We show such toxins compete for ERAD to rescue endogenous misfolded proteins. Cholera toxin or verotoxin (Shiga toxin) containing genetically inactivated (± an N-terminal polyleucine tail) A subunit can, within 2-4 hrs, temporarily increase F508delCFTR protein, the major cystic fibrosis (CF) mutant (5-10x), F508delCFTR Golgi maturation (<10x), cell surface expression (20x) and chloride transport (2x) in F508del CFTR transfected cells and patient-derived F508delCFTR bronchiolar epithelia, without apparent cytopathology. These toxoids also increase glucocerobrosidase (GCC) in N370SGCC Gaucher Disease fibroblasts (3x), another ERAD-exacerbated misfiling disease. We identify a new, potentially benign approach to the treatment of certain genetic protein misfolding diseases.
[Mh] Termos MeSH primário: Degradação Associada com o Retículo Endoplasmático/efeitos dos fármacos
Retículo Endoplasmático/efeitos dos fármacos
Dobramento de Proteína/efeitos dos fármacos
Toxinas Biológicas/farmacologia
[Mh] Termos MeSH secundário: Western Blotting
Toxina da Cólera/farmacologia
Fibrose Cística/genética
Fibrose Cística/metabolismo
Fibrose Cística/prevenção & controle
Regulador de Condutância Transmembrana em Fibrose Cística/química
Regulador de Condutância Transmembrana em Fibrose Cística/genética
Regulador de Condutância Transmembrana em Fibrose Cística/metabolismo
Retículo Endoplasmático/metabolismo
Células HEK293
Seres Humanos
Microscopia de Fluorescência
Modelos Biológicos
Mutação
Transporte Proteico/efeitos dos fármacos
Deficiências na Proteostase/genética
Deficiências na Proteostase/metabolismo
Deficiências na Proteostase/prevenção & controle
Toxinas Shiga/farmacologia
Toxinas Biológicas/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Shiga Toxins); 0 (Toxins, Biological); 126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator); 9012-63-9 (Cholera Toxin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166948



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