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| [PMID]: | 27777194 |
| [Au] Autor: | Huang H; Tian ZT; Yao YM; Li TS |
| [Ad] Endereço: | 1Emergency Department, Chinese PLA General Hospital, Beijing 100853, China; 2Department of Critical Care Medicine, General Hospital of Jinan Command, Jinan 250031, China. E-mail: huanghe_hh@126.com. |
| [Ti] Título: | [Tumor necrosis factor-α-induced protein 8 like-2 promotes apoptosis of CD4 T lymphocytes in mice with severe burn injury]. |
| [So] Source: | Nan Fang Yi Ke Da Xue Xue Bao;36(10):1334-1339, 2016 Oct 20. | | [Is] ISSN: | 1673-4254 |
| [Cp] País de publicação: | China |
| [La] Idioma: | chi |
| [Ab] Resumo: | OBJECTIVE: To investigate the effect of tumor necrosis factor-α-induced protein 8 like-2 (TIPE2) on apoptosis of CD4 T lymphocytes in a murine model of severe burn injury. METHODS: A total of 140 male mice were randomly allocated into 6 groups. Small RNA interference technique was used to construct a siTIPE2-overexpressing lentivirus, and severe burn injury models were established in the mice. CD4 T cells were purified from spleen of the mice, and the expressions of TIPE2, Smad2/Smad3, P-Smad2/P-Smad3 and Bcl-2/Bimprotein in CD4 Tregs were detected. The changes in mitochondrial membrane potential and cytochrome C in CD4 T cells were detected, and the activities of caspase-3, caspase-8, and caspase-9 were analyzed. RESULTS: Down-regulation of TIPE2 promoted the apoptosis of CD4 T lymphocytes in siTIPE2-burn group, in which the protein expressions of P-smad2/P-Smad3 decreased, Bcl-2 expression increased and Bim expression decreased significantly as compared with the other groups (P<0.01 or 0.05). The mitochondrial membrane potential and cytochrome C expression in CD4 T cells were down-regulated in siTIPE2-burn group (P<0.05) with a lowered caspase-3 activity compared with TIPE2-burn group (P<0.01) and decreased caspase-8 and caspase-9 compared with the other groups (P<0.05). The apoptosis rate was the highest in TIPE2-burn group, whose Smad2/Smad3 was higher than that in the sham group (P<0.05) and the expression of P-smad2/P-Smad3 significantly increased compared with the other groups (P<0.05). In TIPE2-burn group, the mitochondrial membrane potential in CD4 T cells was decreased (P<0.01), the expression of cytochrome C increased markedly (P<0.01), and the activities of caspase-3, caspase-8, and caspase-9 were all obviously higher than those in the other groups (P<0.05). CONCLUSION: As an important immunoregulatory molecule, TIPE2 can promote the apoptosis of CD4 T lymphocyte in mice with sever burn injury. |
| [Mh] Termos MeSH primário: |
Apoptose
Queimaduras/imunologia
Linfócitos T CD4-Positivos/citologia
Peptídeos e Proteínas de Sinalização Intracelular/metabolismo
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| [Mh] Termos MeSH secundário: |
Animais
Caspase 3/metabolismo
Caspase 8/metabolismo
Caspase 9/metabolismo
Regulação para Baixo
Masculino
Camundongos
Proteína Smad2/metabolismo
Proteína Smad3/metabolismo
Baço
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| [Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Intracellular Signaling Peptides and Proteins); 0 (Smad2 Protein); 0 (Smad2 protein, mouse); 0 (Smad3 Protein); 0 (Smad3 protein, mouse); 0 (TIPE2 protein, mouse); EC 3.4.22.- (Casp3 protein, mouse); EC 3.4.22.- (Casp8 protein, mouse); EC 3.4.22.- (Casp9 protein, mouse); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 8); EC 3.4.22.- (Caspase 9) |
| [Em] Mês de entrada: | 1711 |
| [Cu] Atualização por classe: | 171128 |
[Lr] Data última revisão:
| 171128 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 161026 |
| [St] Status: | MEDLINE |
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