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[PMID]:27901202
[Au] Autor:Atalayin C; Tezel H; Dagci T; Karabay Yavasoglu NU; Oktem G; Kose T
[Ad] Endereço:Ege University, School of Dentistry, Department of Restorative Dentistry, Izmir, Turkey.
[Ti] Título:In vivo performance of different scaffolds for dental pulp stem cells induced for odontogenic differentiation.
[So] Source:Braz Oral Res;30(1):e120, 2016 Nov 28.
[Is] ISSN:1807-3107
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. Mesenchymal stem cell medium was used in the control groups. DPSC were transplanted onto the backs of mice via three scaffolds: copolymer of L-lactide and DL-lactide (PLDL), copolymer of DL-lactide (PDL) and hydroxyapatite tricalcium phosphate (HA/TCP). The expression levels of dentin sialo-phosphoprotein (DSPP), dentin matrix protein-1 (DMP1), enamelysin/matrix metalloproteinase 20 (MMP20) and phosphate-regulating gene with homologies to endopeptidases on X chromosome (PHEX) were analysed using RT-PCR. The expressions in the experimental groups were compared to those in the control groups. The transcript expressions at 6 and 12 weeks were significantly different for all scaffolds (p < 0.05), except for the expression of DSPP in the PLDL group with regard to the time variable. Although there was a decrease in the expression of enamelysin/MMP20 in PLDL and HA/TCP at 12 weeks, all other expressions increased and reached their highest level at 12 weeks. The highest DSPP expression was in the PDL group (p < 0.05). The highest expression of DMP1 was detected in the HA/TCP group (p < 0.05). The highest expression of PHEX was in the PLDL group (p < 0.05). Consequently, PLDL and PDL seemed to be promising scaffold candidates for odontogenic regeneration at least as HA-TCP, when they were applied with the DPSC induced for odontogenic differentiation.
[Mh] Termos MeSH primário: Diferenciação Celular/fisiologia
Polpa Dentária/citologia
Polímeros/química
Células-Tronco/fisiologia
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/química
Fosfatos de Cálcio/química
Técnicas de Cultura de Células
Esmalte Dentário/química
Dentina/química
Dioxanos/química
Durapatita/química
Proteínas da Matriz Extracelular/análise
Expressão Gênica
Seres Humanos
Metaloproteinase 20 da Matriz/análise
Camundongos
Endopeptidase Neutra Reguladora de Fosfato PHEX/análise
Fosfoproteínas/análise
Reprodutibilidade dos Testes
Sialoglicoproteínas/análise
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Calcium Phosphates); 0 (DMP1 protein, human); 0 (Dioxanes); 0 (Extracellular Matrix Proteins); 0 (Phosphoproteins); 0 (Polymers); 0 (Sialoglycoproteins); 0 (dentin sialophosphoprotein); 91D9GV0Z28 (Durapatite); 95-96-5 (dilactide); EC 3.4.24.- (Matrix Metalloproteinase 20); EC 3.4.24.- (PHEX Phosphate Regulating Neutral Endopeptidase); EC 3.4.24.- (PHEX protein, human); K4C08XP666 (tricalcium phosphate)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE


  2 / 166 MEDLINE  
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[PMID]:27666430
[Au] Autor:Ogbureke KU; Koli K; Saxena G
[Ad] Endereço:Department of Diagnostic and Biomedical Sciences, School of Dentistry, University of Texas Health Science Center at Houston, Houston, Texas (KUEO, KK, GS).
[Ti] Título:Matrix Metalloproteinase 20 Co-expression With Dentin Sialophosphoprotein in Human and Monkey Kidneys.
[So] Source:J Histochem Cytochem;64(10):623-36, 2016 10.
[Is] ISSN:1551-5044
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We recently reported the expression of matrix metalloproteinase 20 (MMP20), hitherto thought to be tooth specific, in the metabolically active ductal epithelial cells of human salivary glands. Furthermore, our report indicated that MMP20 co-expressed and potentially interacts with dentin sialophosphoprotein (DSPP), a member of the small integrin-binding ligand N-linked glycoproteins (SIBLINGs). Our earlier reports have shown the co-expression of three MMPs, MMP2, MMP3, and MMP9, with specific members of the SIBLING family: bone sialoprotein, osteopontin, and dentin matrix protein 1, respectively. This study investigated the expression of MMP20 and verified its co-expression with DSPP in human and monkey kidney sections and human mixed renal cells by IHC, in situ proximity ligation assay, and immunofluorescence. Our results show that MMP20 is expressed in all segments of the human and monkey nephron with marked intensity in the proximal and distal tubules, and was absent in the glomeruli. Furthermore, MMP20 co-expressed with DSPP in the proximal, distal, and collecting tubules, and in mixed renal cells. Consistent with other SIBLING-MMP pairs, the DSPP-MMP20 pair may play a role in the normal turnover of cell surface proteins and/or repair of pericellular matrix proteins of the basement membranes in the metabolically active duct epithelial system of the nephrons.
[Mh] Termos MeSH primário: Proteínas da Matriz Extracelular/metabolismo
Rim/metabolismo
Metaloproteinase 20 da Matriz/metabolismo
Fosfoproteínas/metabolismo
Sialoglicoproteínas/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Seres Humanos
Macaca fascicularis
Néfrons/metabolismo
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Extracellular Matrix Proteins); 0 (Phosphoproteins); 0 (Sialoglycoproteins); 0 (dentin sialophosphoprotein); EC 3.4.24.- (Matrix Metalloproteinase 20)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160927
[St] Status:MEDLINE
[do] DOI:10.1369/0022155416665098


  3 / 166 MEDLINE  
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[PMID]:27558264
[Au] Autor:Kwak SY; Yamakoshi Y; Simmer JP; Margolis HC
[Ad] Endereço:Center for Biomineralization, Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA.
[Ti] Título:MMP20 Proteolysis of Native Amelogenin Regulates Mineralization In Vitro.
[So] Source:J Dent Res;95(13):1511-1517, 2016 Dec.
[Is] ISSN:1544-0591
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent studies have shown that native phosphorylated full-length porcine amelogenin (P173) and its predominant cleavage product (P148) can inhibit spontaneous calcium phosphate formation in vitro by stabilizing an amorphous calcium phosphate (ACP) precursor phase. Since full-length amelogenin undergoes proteolysis by matrix metalloproteinase 20 (MMP20, enamelysin) soon after secretion, the present study was conducted to assess the effect of amelogenin proteolysis on calcium phosphate formation. Calcium and phosphate were sequentially added to protein solutions without and with added MMP20 (ratio = 200:1) under physiological-like conditions of ionic strength (163 mM) in 50 mM Tris-HCl (pH 7.4) at 37 °C. Protein degradation with time was assessed by gel-electrophoresis, and mineral products formed were characterized by transmission electron microscopy (TEM). MMP20 was found to cleave P173 to primarily generate P148, along with P162, P46-148, and P63/64-148. In sharp contrast, MMP20 did not cleave P148. In addition, the formation of well-aligned bundles of enamel-like hydroxyapatite (HA) crystals was promoted in the presence of P173 with added MMP20, while only ACP particles were seen in the absence of MMP20. Although P148 was found to have a somewhat lower capacity to stabilize ACP and prevent HA formation compared with P173 in the absence of MMP20, essentially no HA formation was observed in the presence of somewhat higher concentrations of P148 regardless of MMP20 addition, due to the lack of observed protein proteolysis. Present findings suggest that ACP transformation to ordered arrays of enamel crystals may be regulated in part by the proteolysis of full-length native amelogenin, while the predominant amelogenin degradation product in developing enamel (e.g., P148) primarily serves to prevent uncontrolled mineral formation during the secretory stage of amelogenesis.
[Mh] Termos MeSH primário: Amelogênese/fisiologia
Metaloproteinase 20 da Matriz/metabolismo
Proteólise
[Mh] Termos MeSH secundário: Amelogenina
Animais
Fosfatos de Cálcio
Eletroforese em Gel de Poliacrilamida
Concentração de Íons de Hidrogênio
Técnicas In Vitro
Microscopia Eletrônica de Transmissão
Fosforilação
Soluções
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amelogenin); 0 (Calcium Phosphates); 0 (Solutions); 97Z1WI3NDX (calcium phosphate); EC 3.4.24.- (Matrix Metalloproteinase 20)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:160826
[St] Status:MEDLINE


  4 / 166 MEDLINE  
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[PMID]:27513226
[Au] Autor:Rajasekaran S; Kanna RM; Reddy RR; Natesan S; Raveendran M; Cheung KM; Chan D; Kao PY; Yee A; Shetty AP
[Ad] Endereço:*Department of Orthopaedics and Spine Surgery, Ganga Hospital †Tamilnadu Agricultural University, Coimbatore, Tamil Nadu, India ‡University of Hong Kong, Hong Kong.
[Ti] Título:How Reliable Are the Reported Genetic Associations in Disc Degeneration?: The Influence of Phenotypes, Age, Population Size, and Inclusion Sequence in 809 Patients.
[So] Source:Spine (Phila Pa 1976);41(21):1649-1660, 2016 Nov 01.
[Is] ISSN:1528-1159
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:STUDY DESIGN: Prospective genetic association study. OBJECTIVE: The aim of this study was to document the variations in the genetic associations, when different magnetic resonance imaging (MRI) phenotypes, age stratification, cohort size, and sequence of cohort inclusion are varied in the same study population. SUMMARY OF BACKGROUND DATA: Genetic associations with disc degeneration have shown high inconsistency, generally attributed to hereditary factors and ethnic variations. However, the effect of different phenotypes, size of the study population, age of the cohort, etc have not been documented clearly. METHODS: Seventy-one single-nucleotide polymorphisms (SNPs) of 41 candidate genes were correlated to six MRI markers of disc degeneration (annular tears, Pfirmann grading, Schmorl nodes, Modic changes, Total Endplate Damage score, and disc bulge) in 809 patients with back pain and/or sciatica. In the same study group, the correlations were then retested for different age groups, different sample, size and sequence of subject inclusion (first 404 and the second 405) and the differences documented. RESULTS: The mean age of population (M: 455, F: 354) was 36.7 ±â€Š10.8 years. Different genetic associations were found with different phenotypes: disc bulge with three SNPs of CILP; annular tears with rs2249350 of ADAMTS5 and rs11247361 IGF1R; modic changes with VDR and MMP20; Pfirmann grading with three SNPs of MMP20 and Schmorl node with SNPs of CALM1 and FN1 and none with Total End Plate Score.Subgroup analysis based on three age groups and dividing the total population into two groups also completely changed the associations for all the six radiographic parameters. CONCLUSION: In the same study population, SNP associations completely change with different phenotypes. Variations in age, inclusion sequence, and sample size resulted in change of genetic associations. Our study questions the validity of previous studies and necessitates the need for standardizing the description of disc degeneration, phenotype selection, study sample size, age, and other variables in future studies. LEVEL OF EVIDENCE: 4.
[Mh] Termos MeSH primário: Predisposição Genética para Doença
Degeneração do Disco Intervertebral/genética
Disco Intervertebral/diagnóstico por imagem
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Proteína ADAMTS5/genética
Adulto
Fatores Etários
Estudos Transversais
Proteínas da Matriz Extracelular/genética
Feminino
Estudos de Associação Genética
Seres Humanos
Degeneração do Disco Intervertebral/diagnóstico por imagem
Imagem por Ressonância Magnética
Masculino
Metaloproteinase 20 da Matriz/genética
Meia-Idade
Fenótipo
Pirofosfatases/genética
Receptores de Calcitriol/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Extracellular Matrix Proteins); 0 (Receptors, Calcitriol); EC 3.4.24.- (ADAMTS5 Protein); EC 3.4.24.- (Matrix Metalloproteinase 20); EC 3.6.1.- (CILP protein, human); EC 3.6.1.- (Pyrophosphatases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160812
[St] Status:MEDLINE


  5 / 166 MEDLINE  
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[PMID]:27387974
[Au] Autor:Kawamura R; Hayashi Y; Murakami H; Nakashima M
[Ad] Endereço:Department of Stem Cell Biology and Regenerative Medicine, National Center for Geriatrics and Gerontology, Research Institute, 7-430 Morioka, Obu, Aichi, 474-8511, Japan.
[Ti] Título:EDTA soluble chemical components and the conditioned medium from mobilized dental pulp stem cells contain an inductive microenvironment, promoting cell proliferation, migration, and odontoblastic differentiation.
[So] Source:Stem Cell Res Ther;7(1):77, 2016 May 25.
[Is] ISSN:1757-6512
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The critical challenge in tissue engineering is to establish an optimal combination of stem cells, signaling morphogenetic molecules, and extracellular matrix scaffold/microenvironment. The extracellular matrix components of teeth may be reconstituted as an inductive microenvironment in an ectopic tooth transplantation bioassay. Thus, the isolation and identification of the chemical components of the inductive microenvironment in pulp/dentin regeneration will accelerate progress towards the goal of tissue engineering of the tooth. METHODS: The teeth demineralized in 0.6 M hydrochloric acid were sequentially extracted by 4.0 M guanidine hydrochloride (GdnHCl), pH 7.4, and 0.5 M ethylenediaminetetraacetic acid (EDTA), pH 7.4. The extracted teeth were transplanted into an ectopic site in severe combined immunodeficiency (SCID) mice with mobilized dental pulp stem cells (MDPSCs). The unextracted tooth served as a positive control. Furthermore, the soluble components for the inductive microenvironment, the GdnHCl extracts, or the EDTA extracts together with or without MDPSC conditioned medium (CM) were reconstituted systematically with autoclaved teeth in which the chemical components were completely inactivated and only the physical microenvironment was preserved. Their pulp/dentin regenerative potential and angiogenic potential were compared 28 days after ectopic tooth transplantation by histomorphometry and real-time RT-PCR analysis. RESULTS: Expression of an odontoblastic marker, enamelysin, and a pulp marker, thyrotropin-releasing hormone degrading enzyme (TRH-DE), was lower, and expression of a periodontal cell marker, anti-asporin/periodontal ligament-associated protein 1 (PLAP-1), was higher in the transplant of the EDTA-extracted teeth compared with the GdnHCl-extracted teeth. The autoclaved teeth reconstituted with the GdnHCl extracts or the EDTA extracts have weak regenerative potential and minimal angiogenic potential, and the CM significantly increased this potential. Combinatorial effects of the EDTA extracts and the CM on pulp/dentin regeneration were demonstrated in vivo, consistent with their in-vitro effects on enhanced proliferation, migration, and odontoblastic differentiation. CONCLUSIONS: The EDTA-extracted teeth demonstrated significantly lower pulp/dentin regenerative potential compared with the GdnHCl-extracted teeth. The EDTA soluble chemical components when reconstituted with the physical structure of autoclaved teeth serve as an inductive microenvironment for pulp/dentin regeneration, promoting cell proliferation, migration, and odontoblastic differentiation.
[Mh] Termos MeSH primário: Dente Pré-Molar/transplante
Meios de Cultivo Condicionados/farmacologia
Células Mesenquimais Estromais/efeitos dos fármacos
Neovascularização Fisiológica/efeitos dos fármacos
Odontoblastos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Aminopeptidases/genética
Aminopeptidases/metabolismo
Animais
Dente Pré-Molar/citologia
Dente Pré-Molar/metabolismo
Biomarcadores/metabolismo
Diferenciação Celular/efeitos dos fármacos
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Microambiente Celular
Meios de Cultivo Condicionados/isolamento & purificação
Polpa Dentária/citologia
Polpa Dentária/metabolismo
Proteínas da Matriz Extracelular/genética
Proteínas da Matriz Extracelular/metabolismo
Regulação da Expressão Gênica
Metaloproteinase 20 da Matriz/genética
Metaloproteinase 20 da Matriz/metabolismo
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/metabolismo
Camundongos
Camundongos SCID
Odontoblastos/citologia
Odontoblastos/metabolismo
Fosfoproteínas/genética
Fosfoproteínas/metabolismo
Cultura Primária de Células
Ácido Pirrolidonocarboxílico/análogos & derivados
Ácido Pirrolidonocarboxílico/metabolismo
Sialoglicoproteínas/genética
Sialoglicoproteínas/metabolismo
Transdução de Sinais
Suínos
Engenharia Tecidual
Transplante Heterólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Culture Media, Conditioned); 0 (Extracellular Matrix Proteins); 0 (Phosphoproteins); 0 (Sialoglycoproteins); 0 (dentin sialophosphoprotein); EC 3.4.11.- (Aminopeptidases); EC 3.4.19.6 (pyroglutamyl-peptidase II); EC 3.4.24.- (Matrix Metalloproteinase 20); SZB83O1W42 (Pyrrolidonecarboxylic Acid)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE
[do] DOI:10.1186/s13287-016-0334-z


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[PMID]:27365112
[Au] Autor:Shahid M; Balto HA; Al-Hammad N; Joshi S; Khalil HS; Somily AM; Sinjilawi NA; Al-Ghamdi S; Faiyaz-Ul-Haque M; Dhillon VS
[Ad] Endereço:Department of Biochemistry and Molecular Biology, College of Medicine, Prince Sattam Bin Abdulaziz University, Alkharj, Saudi Arabia. Electronic address: dr.shahid90@yahoo.com.
[Ti] Título:Mutations in MSX1, PAX9 and MMP20 genes in Saudi Arabian patients with tooth agenesis.
[So] Source:Eur J Med Genet;59(8):377-85, 2016 Aug.
[Is] ISSN:1878-0849
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Tooth agenesis in human being is the most common congenital anomaly associated with dental development. Mutations in many genes such as MSH homeobox 1 (MSX1), paired box gene 9 (PAX9), ectodysplasin A (EDA) and EDA receptor (EDAR) have been associated with familial form of this condition. However, in large majority of patients, genetic cause could not be identified. The primary aim of present study was to identify the causative mutation(s) in these genes in Saudi Arabian families diagnosed with non-syndromic form of disease. Direct sequencing of coding regions, including exon-intron boundaries of these genes was carried out. All identified nucleotide variations were also tested to exclude possibility of being rare polymorphisms. The sequence analysis of exons and exon-intronic regions of these genes revealed five new mutations that include four in MSX1, one in PAX9 and one single nucleotide polymorphism (SNP) in majority of the patients in MMP20. One novel mutation in exon 1 of MSX1 gene (5354C > G; A40G) was found in three patients. In addition, another novel mutation was detected in two patients in exon 3 (PAX9) as g.10672A > T which changes asparagine to isoleucine at position 40. These mutations were not found in any of the control subjects. A single SNP in MMP20 genes (g.5066A > C) that changes lysine to threonine at position 18 was found in 10% controls as well. Our results for the first time demonstrates that mutations in MSX1 gene might play an important role in hypodontia cases involving pre-molars and is a risk factor for this ethnic population mainly of Arabs and is first report linking these mutations with tooth agenesis.
[Mh] Termos MeSH primário: Anodontia/diagnóstico
Anodontia/genética
Fator de Transcrição MSX1/genética
Metaloproteinase 20 da Matriz/genética
Mutação
Fator de Transcrição PAX9/genética
[Mh] Termos MeSH secundário: Adolescente
Adulto
Alelos
Sequência de Aminoácidos
Criança
Biologia Computacional/métodos
Análise Mutacional de DNA
Éxons
Feminino
Ordem dos Genes
Genótipo
Seres Humanos
Masculino
Meia-Idade
Fenótipo
Polimorfismo de Nucleotídeo Único
Arábia Saudita
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MSX1 Transcription Factor); 0 (MSX1 protein, human); 0 (PAX9 Transcription Factor); 0 (PAX9 protein, human); EC 3.4.24.- (Matrix Metalloproteinase 20)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160702
[St] Status:MEDLINE


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[PMID]:27272780
[Au] Autor:Kraus D; Reckenbeil J; Perner S; Winter J; Probstmeier R
[Ad] Endereço:Department of Prosthodontics, Preclinical Education and Material Science, University of Bonn, Bonn, Germany.
[Ti] Título:Expression Pattern of Matrix Metalloproteinase 20 (MMP20) in Human Tumors.
[So] Source:Anticancer Res;36(6):2713-8, 2016 Jun.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIM: Matrix metalloproteinase 20 (MMP20) is a member of the family of matrix metalloproteinases. Under normal conditions the expression of MMP20 is restricted to ameloblasts and odontoblasts. In order to identify a possible expression of MMP20 under pathological conditions, we investigated three major human tumor entities, i.e. colon, breast and lung tumors, on the mRNA and protein level. MATERIALS AND METHODS: Real-time RT-PCR and immunocytochemical analyses of established human tumor cell lines were employed for our study; immunohistochemical analysis was performed on both primary tumors and normal control tissues. RESULTS: MMP20 was identified on both the mRNA and the protein level in breast MCF-7, colon HT-29, and lung A549 cell lines. MMP20 was also detected in primary tumor tissue by immunohistochemistry. CONCLUSION: MMP20 is a new potential candidate for tumor diagnosis or therapy.
[Mh] Termos MeSH primário: Metaloproteinase 20 da Matriz/análise
Neoplasias/enzimologia
[Mh] Termos MeSH secundário: Seres Humanos
Imuno-Histoquímica
Células MCF-7
Metaloproteinase 20 da Matriz/genética
Metaloproteinase 20 da Matriz/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.4.24.- (MMP20 protein, human); EC 3.4.24.- (Matrix Metalloproteinase 20)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE


  8 / 166 MEDLINE  
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[PMID]:26995836
[Ti] Título:Promising Gel for Regrowing Enamel.
[So] Source:Dent Today;35(2):52, 2016 Feb.
[Is] ISSN:8750-2186
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Amelogenina/uso terapêutico
Materiais Biocompatíveis/uso terapêutico
Quitosana/uso terapêutico
Esmalte Dentário/efeitos dos fármacos
Metaloproteinase 20 da Matriz/uso terapêutico
[Mh] Termos MeSH secundário: Amelogênese/efeitos dos fármacos
Cristalização
Seres Humanos
[Pt] Tipo de publicação:NEWS
[Nm] Nome de substância:
0 (Amelogenin); 0 (Biocompatible Materials); 9012-76-4 (Chitosan); EC 3.4.24.- (MMP20 protein, human); EC 3.4.24.- (Matrix Metalloproteinase 20)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:160321
[Lr] Data última revisão:
160321
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:160322
[St] Status:MEDLINE


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[PMID]:26981753
[Au] Autor:Atalayin C; Tezel H; Dagci T; Yavasoglu NU; Oktem G
[Ad] Endereço:Department of Restorative Dentistry, School of Dentistry, Ege University, Izmir, Turkey.
[Ti] Título:Medium modification with bone morphogenetic protein 2 addition for odontogenic differentiation.
[So] Source:Braz Oral Res;30, 2016.
[Is] ISSN:1807-3107
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate whether medium modification improves the odontogenic differentiation of human dental pulp stem cells (DPSC) in vitro and in vivo. DPSC isolated from human impacted third molar teeth were analysed for clusters of differentiation with flow cytometry. Odontogenic differentiation was stimulated by medium modification with the addition of bone morphogenetic protein 2 (BMP2). The expression of dentin sialophosphoprotein, dentin matrix protein 1, enamelysin/matrix metalloproteinase 20 and the phosphate-regulating gene with homologies to endopeptidases on the X chromosome of the cells were analysed with RT-PCR at 7, 14 and 21 days. Then, DPSC were transplanted on the back of immunocompromised mice via a hydroxyapatite tricalcium phosphate scaffold, and the structure of the formed tissue was investigated. The cells were identified as mesenchymal stem cells with a 98.3% CD73 and CD90 double-positive cell rate. The increase in mineralization capacity and expression of human enamel-dentin specific transcripts proportional to the culture period were determined after differentiation. Six weeks after transplantation, an osteo-dentin matrix was formed in the group in which odontogenic differentiation was stimulated, and the odontogenic characteristics of the matrix were confirmed by histological examination and RT-PCR analysis. Odontogenic differentiation of the isolated and characterized human DPSC was improved with medium modification by the addition of BMP2 in vitro and in vivo. The defined medium and applied technique have a potential use for forming reparative dentin in the future, but the effects of the method should be investigated in long-term studies.
[Mh] Termos MeSH primário: Proteína Morfogenética Óssea 2/farmacologia
Diferenciação Celular/efeitos dos fármacos
Meios de Cultura/química
Polpa Dentária/citologia
Células-Tronco/citologia
[Mh] Termos MeSH secundário: Actinas/análise
Adulto
Animais
Proteína Morfogenética Óssea 2/química
Diferenciação Celular/fisiologia
Proliferação Celular/efeitos dos fármacos
Proliferação Celular/fisiologia
Células Cultivadas
Proteínas da Matriz Extracelular/análise
Citometria de Fluxo
Seres Humanos
Metaloproteinase 20 da Matriz/análise
Camundongos
Odontogênese/efeitos dos fármacos
Odontogênese/fisiologia
Endopeptidase Neutra Reguladora de Fosfato PHEX/análise
Fosfoproteínas/análise
Reprodutibilidade dos Testes
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Sialoglicoproteínas/análise
Transplante de Células-Tronco/métodos
Fatores de Tempo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins); 0 (BMP2 protein, human); 0 (Bone Morphogenetic Protein 2); 0 (Culture Media); 0 (Dmp1 protein, mouse); 0 (Extracellular Matrix Proteins); 0 (Phosphoproteins); 0 (Sialoglycoproteins); 0 (dentin sialophosphoprotein); EC 3.4.24.- (Matrix Metalloproteinase 20); EC 3.4.24.- (Mmp20 protein, mouse); EC 3.4.24.- (PHEX Phosphate Regulating Neutral Endopeptidase); EC 3.4.24.- (Phex protein, mouse)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160317
[Lr] Data última revisão:
160317
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:160317
[St] Status:MEDLINE


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[PMID]:26692439
[Au] Autor:Zhai LL; Wu Y; Cai CY; Huang Q; Tang ZG
[Ad] Endereço:From the Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University; and Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, Hefei, People's Republic of China.
[Ti] Título:High-Level Expression and Prognostic Significance of Matrix Metalloprotease-19 and Matrix Metalloprotease-20 in Human Pancreatic Ductal Adenocarcinoma.
[So] Source:Pancreas;45(7):1067-72, 2016 08.
[Is] ISSN:1536-4828
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Matrix metalloproteinase (MMP)-19 and MMP-20 are important members of the MMP family, and their roles in tumor survivorship and progression are continually reported. This work aimed to determine the expression and prognostic significance of MMP-19 and MMP-20 in pancreatic ductal adenocarcinoma (PDAC). METHODS: Immunohistochemistry was used to investigate the levels of MMP-19 and MMP-20 expression in carcinoma tissues and paracancerous tissues from 102 PDAC patients. RESULTS: The MMP-19 and MMP-20 were, respectively, expressed in 71.6% (73/102) and 70.6% (72/102) of carcinoma tissues, and the expression was positively correlated (r = 0.643, P < 0.001). High-level expression of MMP-19 and MMP-20 was strongly correlated with aggressive clinicopathological characteristics. Kaplan-Meier analysis showed that high-level expression of MMP-19 and MMP-20 was significantly associated with decreased event-free survival (P < 0.001) and overall survival (P < 0.001). Multivariate analysis showed that high-level expression of MMP-19 could act as an independent predictive biomarker for poor event-free survival and overall survival. CONCLUSIONS: Levels of MMP-19 and MMP-20 expression are significantly increased in PDAC. High-level expression of MMP-19 and MMP-20 is closely correlated to progression and prognosis of PDAC, and these may be considered as promising markers for unfavorable prognoses.
[Mh] Termos MeSH primário: Carcinoma Ductal Pancreático/metabolismo
Metaloproteinase 20 da Matriz/biossíntese
Metaloproteinases da Matriz Secretadas/biossíntese
Pâncreas/enzimologia
Neoplasias Pancreáticas/metabolismo
[Mh] Termos MeSH secundário: Biomarcadores Tumorais/biossíntese
Carcinoma Ductal Pancreático/patologia
Progressão da Doença
Feminino
Seres Humanos
Imuno-Histoquímica
Estimativa de Kaplan-Meier
Masculino
Meia-Idade
Análise Multivariada
Pâncreas/patologia
Neoplasias Pancreáticas/patologia
Prognóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers, Tumor); EC 3.4.24.- (Matrix Metalloproteinase 20); EC 3.4.24.- (Matrix Metalloproteinases, Secreted); EC 3.4.24.- (matrix metalloproteinase 19)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151223
[St] Status:MEDLINE
[do] DOI:10.1097/MPA.0000000000000569



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