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[PMID]:27890704
[Au] Autor:Pothana L; Devi L; Goel S
[Ad] Endereço:Laboratory for the Conservation of Endangered Species, Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, Hyderabad, 500 007, India.
[Ti] Título:Cryopreservation of adult cervid testes.
[So] Source:Cryobiology;74:103-109, 2017 Feb.
[Is] ISSN:1090-2392
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Several species of cervids are currently classified as threatened or endangered due to a rapid decline in their populations. Sperm cryopreservation, in association with assisted reproductive technologies, can find application for the conservation of endangered cervids. In cases of unsuccessful sperm retrieval through other means prior to the death of the animal, adult testis is the only source of sperm. Recovery of viable sperm from adult testes depends on the effective preservation of testicular tissues through optimization of cryopreservation protocols. The present study evaluated combinations of 10% dimethyl sulfoxide (DMSO) with 0% or 80% fetal bovine serum (FBS) and 20% DMSO with 0 or 20% FBS for the cryopreservation of testicular tissues of three adult cervids using uncontrolled slow freezing protocol. The cryopreserved testis was compared to chilled tissue without cryoprotectants. Results revealed that testicular tissues of barking deer cryopreserved in 20% DMSO (D20) had all the analyzed 7 parameters (number of TNP1-, PRM2 and acrosin-expressing cells/tubule and, the number of viable, morphologically normal, acrosome intact, Annexin V-negative sperm) comparable to the chilled testis. However, testicular tissues of sambhar and hog deer cryopreserved only in D20S20 had 5 of 7 parameters comparable to the chilled testis. In conclusion, D20 is acceptable for cryopreservation of barking deer and D20S20 for sambar and hog deer testes.
[Mh] Termos MeSH primário: Criopreservação/veterinária
Crioprotetores/farmacologia
Cervos
Dimetil Sulfóxido/farmacologia
Preservação do Sêmen/veterinária
Testículo/fisiologia
[Mh] Termos MeSH secundário: Acrosina/metabolismo
Acrossomo/fisiologia
Animais
Proteínas Cromossômicas não Histona/metabolismo
Criopreservação/métodos
Masculino
Protaminas/metabolismo
Preservação do Sêmen/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromosomal Proteins, Non-Histone); 0 (Cryoprotective Agents); 0 (Protamines); 0 (protamine 2); 0 (spermatid transition proteins); EC 3.4.21.10 (Acrosin); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161129
[St] Status:MEDLINE


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[PMID]:27748829
[Au] Autor:Cui X; Jing X; Wu X; Yan M
[Ad] Endereço:Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi, Taiyuan, Shanxi 030000, P.R. China.
[Ti] Título:Protective effect of resveratrol on spermatozoa function in male infertility induced by excess weight and obesity.
[So] Source:Mol Med Rep;14(5):4659-4665, 2016 Nov.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Male infertility is a complex, multifactorial and polygenic disease that contributes to ~50% cases of infertility. Previous studies have demonstrated that excess weight and obesity factors serve an important role in the development of male infertility. An increasing number of studies have reported that resveratrol may regulate the response of cells to specific stimuli that induce cell injury, as well as decrease germ cell apoptosis in mice or rats. In the present study, the semen quality and serum sex hormone levels were evaluated in 324 men, which included 73 underweight, 82 normal weight, 95 overweight and 74 obese men. All patients were referred to The Reproductive Medicine Center of Shanxi Women and Infants Hospital (Taiyuan, China) between January 2013 and January 2015. The aim of the present study was to investigate the effects of resveratrol treatment on the motility, plasma zinc concentration and acrosin activity of sperm from obese males. The sperm concentration, normal sperm morphology, semen volumes, DNA fragmentation rates and testosterone levels in men from the overweight and obese groups were markedly decreased when compared with men in the normal weight group. In addition, the progressive motility, seminal plasma zinc concentration and spermatozoa acrosin activity were notably decreased in the obese group compared with the normal weight group. However, estradiol levels were significantly increased in the overweight, obese and underweight groups compared with the normal weight group. Notably, semen samples from obese males with astenospermia treated with 0­100 µmol/l resveratrol for 30 min demonstrated varying degrees of improvement in sperm motility. When these semen samples were treated with 30 µmol/l resveratrol, sperm motility improved when compared to other doses of resveratrol. Therefore, 30 µmol/l resveratrol was selected for further experiments. Upon treatment of semen samples with resveratrol (30 µmol/l) for 30 min, the seminal plasma zinc concentration and spermatozoa acrosin activity increased significantly in the experimental group compared with the control group. These data suggest that male obesity negatively impacts on male reproductive potential, not only through altering hormone levels, but also by directly altering sperm function. In addition, resveratrol may have a therapeutic and protective effect against obesity-induced abnormalities in semen.
[Mh] Termos MeSH primário: Infertilidade Masculina/etiologia
Obesidade/complicações
Sobrepeso/complicações
Substâncias Protetoras/farmacologia
Espermatozoides/efeitos dos fármacos
Estilbenos/farmacologia
[Mh] Termos MeSH secundário: Acrosina/metabolismo
Adulto
Sobrevivência Celular/efeitos dos fármacos
Citoplasma/metabolismo
Hormônios Esteroides Gonadais/sangue
Seres Humanos
Infertilidade Masculina/epidemiologia
Masculino
Análise do Sêmen
Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gonadal Steroid Hormones); 0 (Protective Agents); 0 (Stilbenes); EC 3.4.21.10 (Acrosin); J41CSQ7QDS (Zinc); Q369O8926L (resveratrol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2016.5840


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[PMID]:27221653
[Au] Autor:Cui X; Jing X; Wu X; Wang Z; Li Q
[Ad] Endereço:Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi, Taiyuan, Shanxi 030000, P.R. China.
[Ti] Título:Potential effect of smoking on semen quality through DNA damage and the downregulation of Chk1 in sperm.
[So] Source:Mol Med Rep;14(1):753-61, 2016 Jul.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Previous studies have found that smoking is associated with decreased male fertility via altering the quality of semen. However, the mechanism by which cigarette smoking affects semen quality remains to be fully elucidated. Heavy smoking-induced DNA damage has been reported to correlate with abnormal spermatozoa and male infertility. It has been reported that, in response to DNA damage, activation of the checkpoint kinase 1 (Chk1) facilitates S and G2 checkpoint arrest. The aim of the present study was to investigate the expression levels of Chk1 in sperm cells of smoking and non­smoking men, and to further examine the correlation between DNA fragmentation rates and the expression levels of Chk1 with smoking. The present study was performed on a cohort of 841 smoking men and 287 non­smoking men. In the investigation, sperm concentration, motility, viability, seminal plasma zinc concentration, acrosin activity and sperm DNA fragmentation were examined. The gene and protein expression levels of Chk1 were detected using reverse transcription-quantitative polymerase chain reaction and western blot analyses, respectively. It was observed that the progressive motility of the sperm was significantly decreased in the moderate and heavy smoking groups, whereas no significant changes were observed in the mild smoking group. The sperm in the medium­term smoking group had significantly decreased progressive motility, and the semen concentration, sperm count and progressive motility vitality were markedly decreased in the long­term smoking group. Compared with the non­smoking group, the abnormal head rates in the heavy smoking group and long­term smoking group were significantly increased. The sperm viability and seminal plasma zinc concentration were markedly increased in the smoking group. Increased DNA fragmentation rates were found in the smoking group. The expression of Chk1 was significantly decreased in the smoking group, compared with the non­smoking group. Progressive motility and sperm concentration showed a nonlinear association with the relative mRNA expression of Chk1. However, an inverse association was found between DNA fragmentation rates and the progressive motility and sperm concentration. These data suggested that the decrease of semen quality caused by cigarette smoking was not only correlated with sperm DNA fragmentation rates, but was also correlated with a decline in the expressive level of Chk1. The expression of Chk1 was associated with DNA damage and apoptosis, the reduction of which may lead to decreased sperm repair and increased sperm apoptosis, with a subsequent effect on semen quality.
[Mh] Termos MeSH primário: Quinase do Ponto de Checagem 1/metabolismo
Dano ao DNA
Fumar/efeitos adversos
Espermatozoides/metabolismo
[Mh] Termos MeSH secundário: Acrosina/metabolismo
Sobrevivência Celular
Fragmentação do DNA
Seres Humanos
Infertilidade Masculina/etiologia
Infertilidade Masculina/metabolismo
Masculino
Análise do Sêmen
Contagem de Espermatozoides
Motilidade Espermática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.7.11.1 (Checkpoint Kinase 1); EC 3.4.21.10 (Acrosin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160526
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2016.5318


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[PMID]:27194355
[Au] Autor:Cuasnicú PS; Da Ros VG; Weigel Muñoz M; Cohen DJ
[Ad] Endereço:Instituto de Biología y Medicina Experimental (IBYME-CONICET), Vuelta de Obligado 2490, C1428ADN, Buenos Aires, Argentina. pcuasnicu@ibyme.conicet.gov.ar.
[Ti] Título:Acrosome Reaction as a Preparation for Gamete Fusion.
[So] Source:Adv Anat Embryol Cell Biol;220:159-72, 2016.
[Is] ISSN:0301-5556
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The acrosome reaction (AR) is a universal requisite for sperm-egg fusion. However, whereas through the animal kingdom fusion of spermatozoa with the egg plasma membrane occurs via the inner acrosomal membrane exposed after the AR, in eutherian mammals, gamete fusion takes place through a specialized region of the acrosome known as the equatorial segment (ES) which becomes fusogenic only after the AR is completed. This chapter focuses on the different molecular mechanisms involved in the acquisition of the fusogenicity of the ES after the AR. We provide an update of the knowledge about the proteins proposed to have a role in this process either by modifying cytoskeletal and/or membrane molecules or by relocalizing to the ES after the AR to subsequently participate in gamete fusion.
[Mh] Termos MeSH primário: Reação Acrossômica/genética
Acrossomo/metabolismo
Fusão de Membrana/genética
Capacitação Espermática/genética
Zona Pelúcida/fisiologia
[Mh] Termos MeSH secundário: Acrosina/genética
Acrosina/metabolismo
Acrossomo/química
Animais
Proteínas de Transporte/genética
Proteínas de Transporte/metabolismo
Membrana Celular/química
Membrana Celular/metabolismo
Feminino
Regulação da Expressão Gênica
Imunoglobulinas/genética
Imunoglobulinas/metabolismo
Masculino
Glicoproteínas de Membrana/genética
Glicoproteínas de Membrana/metabolismo
Proteínas de Membrana/genética
Proteínas de Membrana/metabolismo
Camundongos
Proteínas Serina-Treonina Quinases/genética
Proteínas Serina-Treonina Quinases/metabolismo
Proteínas de Plasma Seminal/genética
Proteínas de Plasma Seminal/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Crisp1 protein, mouse); 0 (Immunoglobulins); 0 (Izumo1 protein, mouse); 0 (Membrane Glycoproteins); 0 (Membrane Proteins); 0 (SPESP1 protein, mouse); 0 (Seminal Plasma Proteins); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (Tssk6 protein, mouse); EC 3.4.21.10 (Acrosin)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160519
[Lr] Data última revisão:
160519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160520
[St] Status:MEDLINE
[do] DOI:10.1007/978-3-319-30567-7_9


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[PMID]:26748928
[Au] Autor:Ito C; Toshimori K
[Ad] Endereço:Department of Reproductive Biology and Medicine, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan. chizuru@faculty.chiba-u.jp.
[Ti] Título:Acrosome markers of human sperm.
[So] Source:Anat Sci Int;91(2):128-42, 2016 Mar.
[Is] ISSN:1447-073X
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Molecular biomarkers that can assess sperm acrosome status are very useful for evaluating sperm quality in the field of assisted reproductive technology. In this review, we introduce and discuss the localization and function of acrosomal proteins that have been well studied. Journal databases were searched using keywords, including "human acrosome", "localization", "fertilization-related protein", "acrosomal membrane", "acrosomal matrix", "acrosome reaction", "knockout mouse", and "acrosome marker".
[Mh] Termos MeSH primário: Acrossomo
Fertilização
Espermatozoides
[Mh] Termos MeSH secundário: Proteínas ADAM
Acrosina
Acrossomo/fisiologia
Reação Acrossômica
Animais
Antígenos
Proteínas de Transporte
Proteínas do Ovo
Precursores Enzimáticos
Glicoproteínas
Seres Humanos
Imunoglobulinas
Isoantígenos
Masculino
Proteína Cofatora de Membrana
Proteínas de Membrana
Camundongos
Receptores de Superfície Celular
Proteínas de Plasma Seminal
Espermatozoides/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (ACRV1 protein, human); 0 (Antigens); 0 (CD46 protein, human); 0 (CRISP2 protein, human); 0 (Carrier Proteins); 0 (Egg Proteins); 0 (Enzyme Precursors); 0 (Eqtn protein, mouse); 0 (Glycoproteins); 0 (IZUMO1 protein, human); 0 (Immunoglobulins); 0 (Isoantigens); 0 (Membrane Cofactor Protein); 0 (Membrane Proteins); 0 (Receptors, Cell Surface); 0 (SPACA1 protein, human); 0 (SPACA3 protein, human); 0 (SPESP1 protein, human); 0 (Seminal Plasma Proteins); 0 (ZPBP1 protein, human); 0 (acrin 1); 0 (sp56 protein, mouse); 0 (zonadhesin); EC 3.4.21.- (proacrosin); EC 3.4.21.10 (Acrosin); EC 3.4.24.- (ADAM Proteins); EC 3.4.24.- (ADAM3A protein, human)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160111
[St] Status:MEDLINE
[do] DOI:10.1007/s12565-015-0323-9


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[PMID]:26476242
[Au] Autor:Manásková-Postlerová P; Cozlová N; Dorosh A; Sulc M; Guyonnet B; Jonáková V
[Ad] Endereço:Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic. Electronic address: pavlam@ibt.cas.cz.
[Ti] Título:Acrosin inhibitor detection along the boar epididymis.
[So] Source:Int J Biol Macromol;82:733-9, 2016 Jan.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Epididymal sperm maturation represents a key step in the reproduction process. Spermatozoa are exposed to epididymal fluid components representing the natural environment essential for their post-testicular maturation. Changes in sperm membrane proteins are influenced by proteolytic, glycosylation and deglycosylation enzymes present in the epididymal fluid. Accordingly, the occurrence of inhibitors of these enzymes in the epididymis is very important for the regulation of sperm membrane protein processing. In the present study, we monitored acrosin inhibitor distribution in boar epididymal fluid and in spermatozoa from different segments of the organ. Using specific polyclonal antibody we registered increasing signal of the acrosin inhibitor (AI) from caput to cauda epididymis. Mass spectroscopy examination of the immunoprecipitated acrosin inhibitor (12 kDa) unequivocally identified sperm-associated acrosin inhibitor (SAAI) in the epididymal tissue. Lectin staining showed N-glycosylation in AI from boar epididymis. Protein detection of AI was supported by the results of semi-quantitative RT-PCR showing the presence of mRNA specifically coding for SAAI and similarly increasing throughout the epididymal duct, from its proximal to distal part. Additionally, the immunofluorescence technique showed the AI localization in the secretory tissue of caput, corpus and cauda epididymis, and in the acrosome region and midpiece of the sperm.
[Mh] Termos MeSH primário: Acrosina/antagonistas & inibidores
Inibidores Enzimáticos/metabolismo
Epididimo/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Inibidores Enzimáticos/sangue
Inibidores Enzimáticos/química
Expressão Gênica
Glicosilação
Masculino
Espectrometria de Massas
Dados de Sequência Molecular
Transporte Proteico
Proteólise
Alinhamento de Sequência
Espermatozoides/metabolismo
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Enzyme Inhibitors); EC 3.4.21.10 (Acrosin)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151018
[St] Status:MEDLINE


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[PMID]:26264441
[Au] Autor:Cheng S; Liang X; Wang Y; Jiang Z; Liu Y; Hou W; Li S; Zhang J; Wang Z
[Ad] Endereço:Health Ministry Key Laboratory of Chronobiology, College of Basic Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, PR China.
[Ti] Título:The circadian Clock gene regulates acrosin activity of sperm through serine protease inhibitor A3K.
[So] Source:Exp Biol Med (Maywood);241(2):205-15, 2016 Jan.
[Is] ISSN:1535-3699
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Our previous study found that CLOCK knockdown in the testes of male mice led to a reduced fertility, which might be associated with the lower acrosin activity. In this present study, we examined the differential expression in proteins of CLOCK knockdown sperm. Clock gene expression was knocked down in cells to confirm those differentially expressions and serine protease inhibitor SERPINA3K was identified as a potential target. The up-regulated SERPINA3K revealed an inverse relationship with Clock knockdown. Direct treatment of normal sperm with recombinant SERPINA3K protein inhibited the acrosin activity and reduced in vitro fertilization rate. The luciferase reporter gene assay showed that the down-regulated of Clock gene could activate the Serpina3k promoter, but this activation was not affected by the mutation of E-box core sequence. Co-IP demonstrated a natural interaction between SERPIAN3K and RORs (α and ß). Taken together, these results demonstrated that SERPINA3K is involved in the Clock gene-mediated male fertility by regulating acrosin activity and provide the first evidence that SERPINA3K could be regulated by Clock gene via retinoic acid-related orphan receptor response elements.
[Mh] Termos MeSH primário: Acrosina/biossíntese
Proteínas CLOCK/metabolismo
Relógios Circadianos
Regulação da Expressão Gênica
Serpinas/metabolismo
Espermatozoides/fisiologia
[Mh] Termos MeSH secundário: Animais
Proteínas CLOCK/deficiência
Feminino
Fertilidade
Técnicas de Inativação de Genes
Masculino
Camundongos Endogâmicos ICR
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Serpina3k protein, mouse); 0 (Serpins); EC 2.3.1.48 (CLOCK Proteins); EC 2.3.1.48 (Clock protein, mouse); EC 3.4.21.10 (Acrosin)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150813
[St] Status:MEDLINE
[do] DOI:10.1177/1535370215597199


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[PMID]:27352337
[Au] Autor:Zhang H; Zhao E; Zhang C; Li X
[Ad] Endereço:Department of Urology, The Second Hospital of Harbin Medical University, Harbin, 150086, Heilongjiang, China.
[Ti] Título:The Change of Semen Superoxide Dismutase and Acrosin Activity in the Sterility of Male Patients with Positive Antisperm Antibody.
[So] Source:Cell Biochem Biophys;73(2):451-453, 2015 Nov.
[Is] ISSN:1559-0283
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we report the change of semen superoxide dismutase (SOD) and acrosin activities in the male sterility patients with positive antisperm antibody (AsAb). The activity of SOD was measured by xanthine oxidase assay and sperm acrosin activity was calculated by BAEE/ADH. Our data show that compared with the normal fertility group, the semen SOD activity in AsAb-positive patients was significantly lower. Similarly, the sperm acrosin activity in AsAb-positive patients was also significantly lower. Our results suggest that the sterility resulting from positive AsAb may be related with the changes of semen SOD and sperm acrosin activities.
[Mh] Termos MeSH primário: Acrosina/metabolismo
Sêmen/enzimologia
Superóxido Dismutase/metabolismo
[Mh] Termos MeSH secundário: Adulto
Anticorpos/sangue
Seres Humanos
Infertilidade Masculina
Masculino
Sêmen/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); EC 1.15.1.1 (Superoxide Dismutase); EC 3.4.21.10 (Acrosin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160629
[St] Status:MEDLINE
[do] DOI:10.1007/s12013-015-0663-z


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[PMID]:26597026
[Au] Autor:Kim JT; Jung HJ; Song H; Yoon MJ
[Ad] Endereço:Department of Animal Science and Biotechnology, Kyungpook National University, Sangju 742-711, Republic of Korea.
[Ti] Título:Acrosin-binding protein (ACRBP) in the testes of stallions.
[So] Source:Anim Reprod Sci;163:179-86, 2015 Dec.
[Is] ISSN:1873-2232
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Acrosin Binding Protein (ACRBP) is specifically localized in the acrosome of germ cells of several species, including mice, pigs, guinea pigs, and humans. The main objective of this study was to investigate ACRBP patterns in the germ cells of stallions at different reproductive stages and seminiferous tubule stages using Western blot, immunohistochemistry, and immunocytochemistry techniques. The stallion reproductive stages were classified as follows: pre-pubertal and post-pubertal stages based on the presence/absence of lumen opening in the seminiferous tubules and full spermatogenesis. The protein band associated with the presence of ACRBP appeared at approximately 35-kDa position, indicating that the antibody used in this study recognizes the mature form of ACRBP. During the pre-pubertal stages, immunolabeling did not detect the presence of ACRBP in the germ cells. However, during the post-pubertal stage, immunolabeling of the ACRBP was observed in the pachytene spermatocyte as well as for round, elongating, and elongated spermatids, and in some spermatozoa. In conclusion, the ACRBP can be used as a molecular marker for pachytene spermatocytes, and for round, elongating, and elongated spermatids. The ACRBP can be used to monitor either normal spermatogenesis in the testicular tissues, or germ cell development in vitro. Because the ACRBP is present in the germ cells of stallions that have undergone puberty, it can be used as an indicator for the sexual maturation of stallions.
[Mh] Termos MeSH primário: Acrosina/metabolismo
Proteínas de Transporte/metabolismo
Regulação da Expressão Gênica/fisiologia
Cavalos/fisiologia
Testículo/metabolismo
[Mh] Termos MeSH secundário: Animais
Biomarcadores
Proteínas de Transporte/genética
Masculino
Transporte Proteico
Proteínas de Ligação a RNA/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Carrier Proteins); 0 (RNA-Binding Proteins); EC 3.4.21.10 (Acrosin)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151129
[Lr] Data última revisão:
151129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151125
[St] Status:MEDLINE


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[PMID]:26442303
[Au] Autor:Pu JB; Gao J; Tang XL
[Ti] Título:[Decreases of progressive motility, total motility, and acrosin activity of sperm from oligoasthenoteratospermia males at different time points after sperm activation].
[So] Source:Zhonghua Nan Ke Xue;21(8):733-6, 2015 Aug.
[Is] ISSN:1009-3591
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To investigate the progressive motility, (PR), total motility (progressive + non-progressive motility, PR + NP), and acrosin activity of sperm from normal and infertile men at different time points after sperm activation. METHODS: Based on the 5th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen and the results of modified Papanicolaou staining, we divided the semen samples into groups A (normal, n = 28), B (oligoasthenoteratospermia, n = 30), and C (asthenoteratospermia, n = 32). At 1, 24, and 48 hours after sperm activation, we detected sperm PR and PR + NP by CASA and chemical colorimetry, and determined sperm acrosin activity using the modified Kennedy method. RESULTS: Sperm PR and PR + NP were significantly decreased in all the three groups at 1-24 hours and even more significantly at 24-48 hours after sperm activation as compared with the baseline (P < 0.05). Sperm acrosin activity showed remarkable reduction in group A (P = 0. 013) , even more significant at 1-24 hours than at 24-48 hours after sperm activation, but not in groups B and C (P = 0.519 and 0.979). CONCLUSION: Sperm PR, PR + NP, and acrosin activity are all decreased with the extension of time after sperm activation, each in a specific manner. Examination of sperm acrosin activity should be applied as a routine tool in the assessment of male fertility.
[Mh] Termos MeSH primário: Acrosina/metabolismo
Infertilidade Masculina/fisiopatologia
Motilidade Espermática/fisiologia
Espermatozoides/fisiologia
[Mh] Termos MeSH secundário: Astenozoospermia/metabolismo
Astenozoospermia/fisiopatologia
Biomarcadores/metabolismo
Seres Humanos
Infertilidade Masculina/metabolismo
Masculino
Sêmen
Espermatozoides/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); EC 3.4.21.10 (Acrosin)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151008
[St] Status:MEDLINE



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