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[PMID]:29381751
[Au] Autor:Chea C; Miyauchi M; Inubushi T; Febriyanti Ayuningtyas N; Subarnbhesaj A; Nguyen PT; Shrestha M; Haing S; Ohta K; Takata T
[Ad] Endereço:Department of Oral & Maxillofacial Pathobiology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
[Ti] Título:Molecular mechanism of inhibitory effects of bovine lactoferrin on the growth of oral squamous cell carcinoma.
[So] Source:PLoS One;13(1):e0191683, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Lactoferrin (LF), a member of the transferrin family, recently has been demonstrated to have anticancer effects on various cancers including oral squamous cell carcinoma (OSCC). However, little is known about the underlying mechanisms of its effects on OSCC. Therefore, we aimed to investigate the mechanism of the suppressive effects of bovine LF (bLF) on the growth of OSCC cells. METHODS: In the current study, HSC2, HSC3, HSC4 and normal human oral keratinocytes (RT7) cell lines were tested with bLF 1, 10, and 100 µg/ml. The effects and detail mechanisms of bLF on proliferation and apoptosis of cells were investigated using flow cytometry and western blotting. RESULTS: We found that bLF (1, 10, and 100 µg/ml) induced activation of p53, a tumor suppressor gene, is associated with the induction of cell cycle arrest in G1/S phase and apoptosis in OSCC. Moreover, bLF downregulated the phosphorylation of Akt and activated suppressor of cytokine signaling 3 (SOCS3), thereby attenuating multiple signaling pathways including mTOR/S6K and JAK/STAT3. Interestingly, we revealed that bLF exerted its effect selectively against HSC3 but not on RT7 via different effects on the phosphorylation status of NF-κB and Akt. CONCLUSION: This is the first report showing that bLF selectively suppresses proliferation through mTOR/S6K and JAK/STAT3 pathways and induction of apoptosis in OSCC. This study provides important new findings, which might be useful in the prevention and treatment of OSCC.
[Mh] Termos MeSH primário: Carcinoma de Células Escamosas/patologia
Lactoferrina/farmacologia
Neoplasias Bucais/patologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Ciclo Celular/efeitos dos fármacos
Linhagem Celular Transformada
Proliferação Celular/efeitos dos fármacos
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191683


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[PMID]:28742378
[Au] Autor:Arroyo G; Ortiz Barrientos KA; Lange K; Nave F; Miss Mas G; Lam Aguilar P; Soto Galindo MA
[Ad] Endereço:1 Department of Citohistología, Universidad de San Carlos de Guatemala , Guatemala, Guatemala .
[Ti] Título:Effect of the Various Steps in the Processing of Human Milk in the Concentrations of IgA, IgM, and Lactoferrin.
[So] Source:Breastfeed Med;12(7):443-445, 2017 09.
[Is] ISSN:1556-8342
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Human milk immune components are unique and important for the development of the newborn. Milk processing at the Human Milk Banks (HMB), however, causes partial destruction of immune proteins. The objective of this study was to determine the effects that heating during the milk processing procedure at the HMB had on the concentrations of IgA, IgM, and lactoferrin at three critical points in time. MATERIALS AND METHODS: Fifty milk samples (150 mL) were collected from voluntary donors at the HMB at the Hospital Nacional Pedro de Bethancourt, located in Antigua Guatemala. Samples from three critical points in time during the milk processing procedure were selected for analysis: freezing/thawing I, freezing/thawing II, and pasteurization. IgA, IgM, and lactoferrin concentrations were determined during each critical point and compared with a baseline concentration. RESULTS: After milk processing, IgA, IgM, and lactoferrin mean concentrations were reduced by 30.0%, 36.0%, and 70.0%, respectively (p < 0.001). Reduction of biological activity was mainly attributed to pasteurization for IgA and lactoferrin (p < 0.001); the first freezing/thawing processes before pasteurization showed no significant reduction difference between mean concentrations of IgA (p = 0.160) and lactoferrin (p = 0.345) but showed a significant effect on IgM concentration (p = 0.016), and the second freezing/thawing procedure only showed a significant effect on IgA (p < 0.001). CONCLUSIONS: The effects of milk processing on the immune proteins that were evaluated in this study demonstrated a significant reduction.
[Mh] Termos MeSH primário: Conservação de Alimentos/métodos
Imunoglobulina A/análise
Imunoglobulina M/análise
Lactoferrina/análise
Bancos de Leite
Leite Humano/química
[Mh] Termos MeSH secundário: Feminino
Congelamento
Seres Humanos
Valor Nutritivo
Pasteurização
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Immunoglobulin A); 0 (Immunoglobulin M); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1089/bfm.2016.0154


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[PMID]:29307524
[Au] Autor:Moreno-Expósito L; Illescas-Montes R; Melguizo-Rodríguez L; Ruiz C; Ramos-Torrecillas J; de Luna-Bertos E
[Ad] Endereço:Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada (Spain), Avda, Ilustración, 60, 18016, Spain. Electronic address: almoreno@correo.ugr.es.
[Ti] Título:Multifunctional capacity and therapeutic potential of lactoferrin.
[So] Source:Life Sci;195:61-64, 2018 Feb 15.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Lactoferrin (LF) is a glycoprotein with high functional versatility that is found in most body fluids. The objective of this study was to gather and update information on the properties attributed to LF. According to this review, LF is a good immunomodulatory agent that acts on both innate and adaptive immune responses. It possesses antimicrobial activity against parasites, fungi, and viruses and also has regenerative properties at tissue level and anti-carcinogenic activity. All of these properties endow LF with major therapeutic potential of which little advantage has been taken to date.
[Mh] Termos MeSH primário: Fatores Imunológicos/uso terapêutico
Lactoferrina/fisiologia
Lactoferrina/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/uso terapêutico
Antineoplásicos/uso terapêutico
Seres Humanos
Lactoferrina/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antineoplastic Agents); 0 (Immunologic Factors); 0 (LTF protein, human); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


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[PMID]:29279524
[Au] Autor:Li X; Liang Y; Qiao Z; Yang J; Han P; Zhao B; Li F; Lv H; Guo J; Gao F; Li L
[Ad] Endereço:Department of Cardiology, Hongqi Hospital, Mudanjiang Medical College.
[Ti] Título:Transcriptional Analysis of Endothelial Cell Alternation Induced by Atrial Natriuretic Polypeptide in Human Umbilical Vein Endothelial Cells.
[So] Source:Int Heart J;59(1):197-202, 2018 Jan 27.
[Is] ISSN:1349-3299
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to explore how atrial natriuretic polypeptide (ANP) affects the properties and function of endothelial cells. Gene expression data GSE56976 generated at 0, 1, and 6 hours after ANP incubation in human umbilical vein endothelial cells (HUVEC) was used. Microarray data were preprocessed for differentially expressed genes (DEGs) in each time-dependent group. Next, gene ontology (GO), pathway analysis, and transcriptional regulation were performed. Co-expression clustering analysis of DEGs and functional enrichment analysis of co-expression modules were processed. RT-PCR analysis was performed to validate gene expression. DEGs were obtained and their counts were increased from 0 hours to 6 hours. No overlapping DEGs were obtained among the 3 groups. The DEGs of ANP_6hours, including TGFB2 (transforming growth factor, beta 2), LTF (lactotransferrin/lactoferrin), and ETV7 (Ets variant 7) were mainly related with cell apoptosis and immune responses. The DEGs in the network of ANP_0hour were mainly associated with epithelial ion transport processes. In addition, 3 co-expressed modules were detected. CSF2 (colony stimulating factor 2) and PF4 (platelet factor 4) of the blue module were related with cytolysis, while FXYD1 (FXYD domain containing ion transport regulator 1) and TGFB2 of the yellow module were mainly enriched in ion transport and the ovulation cycle. The expression of TGFB2 obtained by microarray analysis was consistent with that of RT-PCR. Ion transport could be affected promptly after ANP treatment, and subsequently, the cytolysis of vein endothelial cells may be promoted and endothelial permeability would be enhanced, followed by activated immune responses.
[Mh] Termos MeSH primário: Apoptose
Fator Natriurético Atrial/farmacologia
Regulação da Expressão Gênica
Células Endoteliais da Veia Umbilical Humana/metabolismo
Lactoferrina/genética
Proteínas Proto-Oncogênicas c-ets/genética
Fator de Crescimento Transformador beta2/genética
[Mh] Termos MeSH secundário: Células Cultivadas
Perfilação da Expressão Gênica
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana/patologia
Seres Humanos
Lactoferrina/biossíntese
Proteínas Proto-Oncogênicas c-ets/biossíntese
RNA/genética
Reação em Cadeia da Polimerase em Tempo Real
Fator de Crescimento Transformador beta2/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ETV7 protein, human); 0 (LTF protein, human); 0 (Proto-Oncogene Proteins c-ets); 0 (TGFB2 protein, human); 0 (Transforming Growth Factor beta2); 63231-63-0 (RNA); 85637-73-6 (Atrial Natriuretic Factor); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1536/ihj.16-522


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[PMID]:27773805
[Au] Autor:Camilleri M; Sellin JH; Barrett KE
[Ad] Endereço:Clinical Enteric Neuroscience Translational and Epidemiological Research, Division of Gastroenterology and Hepatology, Department of Medicine, Mayo Clinic, Rochester, Minnesota. Electronic address: camilleri.michael@mayo.edu.
[Ti] Título:Pathophysiology, Evaluation, and Management of Chronic Watery Diarrhea.
[So] Source:Gastroenterology;152(3):515-532.e2, 2017 Feb.
[Is] ISSN:1528-0012
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chronic watery diarrhea poses a diagnostic and therapeutic challenge and is often a disabling condition for patients. Although acute diarrhea is likely to be caused by infection, the causes of chronic diarrhea (>4 weeks in duration) are more elusive. We review the pathophysiology, diagnosis, and treatment of chronic diarrhea. Drawing on recent insights into the molecular mechanisms of intestinal epithelial transport and barrier function, we discuss how diarrhea can result from a decrease in luminal solute absorption, an increase in secretion, or both, as well as derangements in barrier properties. We also describe the various extraepithelial factors that activate diarrheal mechanisms. Finally, clinical evaluation and tests used in the assessment of patients presenting with chronic diarrhea are reviewed, and an algorithm guiding therapeutic decisions and pharmacotherapy is presented.
[Mh] Termos MeSH primário: Diarreia/metabolismo
Absorção Intestinal
Secreções Intestinais
Intestinos/metabolismo
[Mh] Termos MeSH secundário: Proteína C-Reativa/metabolismo
Cromograninas/metabolismo
Doença Crônica
Diarreia/diagnóstico
Diarreia/fisiopatologia
Diarreia/terapia
Fezes/química
Motilidade Gastrointestinal
Seres Humanos
Inflamação
Intestinos/fisiopatologia
Síndrome do Intestino Irritável/metabolismo
Lactoferrina/metabolismo
Complexo Antígeno L1 Leucocitário/metabolismo
Concentração Osmolar
Permeabilidade
Prostaglandinas/metabolismo
Serotonina/metabolismo
Substância P/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Chromogranins); 0 (Leukocyte L1 Antigen Complex); 0 (Prostaglandins); 333DO1RDJY (Serotonin); 33507-63-0 (Substance P); 9007-41-4 (C-Reactive Protein); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28873631
[Au] Autor:Li X; Wang X; Xu D; Cao Y; Wang S; Wang B; Sun B; Yuan F; Gao Y
[Ad] Endereço:Beijing Advanced Innovation Center for Food Nutrition and Human Health (BTBU), School of Food & Chemical Engineering, Beijing Engineering and Technology Research Center of Food Additives, Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Key Labora
[Ti] Título:Enhancing physicochemical properties of emulsions by heteroaggregation of oppositely charged lactoferrin coated lutein droplets and whey protein isolate coated DHA droplets.
[So] Source:Food Chem;239:75-85, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The formation and physicochemical stability of mixed functional components (lutein & DHA) emulsions through heteroaggregation were studied. It was formed by controlled heteroaggregation of oppositely charged lutein and DHA droplets coated by cationic lactoferrin (LF) and anionic whey protein isolate (WPI), respectively. Heteroaggregation was induced by mixing the oppositely charged LF-lutein and WPI-DHA emulsions together at pH 6.0. Droplet size, zeta-potential, transmission-physical stability, microrheological behavior and microstructure of the heteroaggregates formed were measured as a function of LF-lutein to WPI-DHA droplet ratio. Lutein degradation and DHA oxidation by measurement of lipid hydroperoxides and thiobarbituric acid reactive substances were determined. Upon mixing the two types of bioactive compounds droplets together, it was found that the largest aggregates and highest physical stability occurred at a droplet ratio of 40% LF-lutein droplets to 60% WPI-DHA droplets. Heteroaggregates formation altered the microrheological properties of the mixed emulsions mainly by the special network structure of the droplets. When LF-coated lutein droplets ratios were more than 30% and less than 60%, the mixed emulsions exhibited distinct decreases in the Mean Square Displacement, which indicated that their limited scope of Brownian motion and stable structure. Mixed emulsions with LF-lutein/WPI-DHA droplets ratio of 4:6 exhibited Macroscopic Viscosity Index with 13 times and Elasticity Index with 3 times of magnitudes higher than the individual emulsions from which they were prepared. Compared with the WPI-DHA emulsion or LF-lutein emulsion, the oxidative stability of the heteroaggregate of LF-lutein/WPI-DHA emulsions was improved. Heteroaggregates formed by oppositely charged bioactive compounds droplets may be useful for creating specific food structures that lead to desirable physicochemical properties, such as microrheological property, physical and chemical stabilities.
[Mh] Termos MeSH primário: Proteínas do Soro do Leite
[Mh] Termos MeSH secundário: Fenômenos Químicos
Emulsões
Concentração de Íons de Hidrogênio
Lactoferrina
Luteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsions); 0 (Whey Proteins); EC 3.4.21.- (Lactoferrin); X72A60C9MT (Lutein)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:29023467
[Au] Autor:Jahan M; Kracht S; Ho Y; Haque Z; Bhattachatyya BN; Wynn PC; Wang B
[Ad] Endereço:Graham Centre for Agricultural Innovation, Charles Sturt University and NSW Department of Primary Industries, Wagga Wagga, NSW, Australia.
[Ti] Título:Dietary lactoferrin supplementation to gilts during gestation and lactation improves pig production and immunity.
[So] Source:PLoS One;12(10):e0185817, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lactoferrin (LF), a sialylated iron-binding glycoprotein, performs multiple beneficial functions including modulating immunity and improves neurodevelopment, health and growth performance. Maternal LF intervention for gilts (first parity sows) on the performance of gilts and their offspring remains unknown. In the current study gilts were fed with a commercial pig feed supplemented with 1g LF /day (treatment group) or 1g milk casein/day (control group) from day 1 post mating throughout pregnancy and lactation for about 135 days. The milk production and body weight gain was monitored. The immunoglobulin concentrations in the serum of gilts and piglets were measured using ELISA. Our study showed that maternal LF supplementation to the gilt (1) significantly increased milk production at different time points (day 1, 3, 7 and 19) of lactation compared to the control (p<0.001); (2) significantly increased body weight gain of their piglets during the first 19 days of life compared to the control group (p<0.05); (3) tended to increase pregnancy rate, litter size and birth weight, number of piglets born alive, and decrease the number of dead and intrauterine growth restriction (IUGR) piglets; (4) significantly increased the concentration of serum IgA in gilt and serum sIgA in piglet (p<0.05). In summary, maternal Lf intervention in gilts can improve milk production, pig production and serum IgA and sIgA levels, and therefore plays a key role in shaping the performance of their progeny.
[Mh] Termos MeSH primário: Ração Animal
Peso Corporal/efeitos dos fármacos
Lactação/efeitos dos fármacos
Lactoferrina/farmacologia
Gravidez/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Caseínas/farmacologia
Feminino
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caseins); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171022
[Lr] Data última revisão:
171022
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185817


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[PMID]:28988537
[Au] Autor:Sokolov AV; Voynova IV; Kostevich VA; Vlasenko AY; Zakharova ET; Vasilyev VB
[Ad] Endereço:Institute of Experimental Medicine, St. Petersburg, 197376, Russia. biochemsokolov@gmail.com.
[Ti] Título:Comparison of Interaction between Ceruloplasmin and Lactoferrin/Transferrin: to Bind or Not to Bind.
[So] Source:Biochemistry (Mosc);82(9):1073-1078, 2017 Sep.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The year 2016 marked the 50th anniversary of the discovery by S. Osaki who first showed that ceruloplasmin (CP, ferro:O -oxidoreductase or ferroxidase) is capable of oxidizing Fe(II) to Fe(III) and favors the incorporation of the latter into transferrin (TF). However, much debate remains in the literature concerning the existence of a complex between the enzyme oxidizing iron and the protein facilitating its transport in plasma. We studied CP in exocrine fluids and demonstrated its high-affinity interaction with transferrin found in breast milk and in lacrimal fluid, i.e. with lactoferrin (LF). Here we present data obtained by comparing the interaction of CP with LF and TF using surface plasmon resonance and Hummel-Dreyer chromatography. Binding of apo-LF within the range of concentrations 1.6-51.3 µM with CP immobilized on a CM5-chip is characterized by K = 1.07 µM. Under similar conditions, the K for apo-TF was measured and appeared to be higher than 51.3 µM. Hummel-Dreyer chromatography of CP with 51 µM apo-LF/apo-TF in the effluent demonstrated the absence of interaction between apo-TF and CP in solution, contrary to efficient interaction between apo-LF and CP. In contrast to LF, the interaction of apo-TF with CP is probably not stable within the physiological range of concentrations of TF.
[Mh] Termos MeSH primário: Ceruloplasmina/metabolismo
Lactoferrina/metabolismo
Leite Humano/química
Lágrimas/química
Transferrina/metabolismo
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Leite Humano/metabolismo
Ligação Proteica
Lágrimas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Transferrin); EC 1.16.3.1 (Ceruloplasmin); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917090115


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[PMID]:28926582
[Au] Autor:Yuan M; Feng C; Wang S; Zhang W; Chen M; Jiang H; Feng X
[Ad] Endereço:Department of Pharmaceutical Analysis, School of pharmacy, China Medical University, Shenyang, PR China.
[Ti] Título:Selection of possible signature peptides for the detection of bovine lactoferrin in infant formulas by LC-MS/MS.
[So] Source:PLoS One;12(9):e0184152, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An LC-MS/MS assay based on a signature peptide was developed and fully validated for the quantitation of bovine lactoferrin in infant formulas. Three unreported signature peptides were derived and identified from the tryptic peptides of bovine lactoferrin. The peptide ETTVFENLPEK was used for quantification based on assay performance. The blank matrix camel milk powder and bovine lactoferrin protein standards were mixed and spiked with stable isotope-labeled internal standard to establish a calibration curve. The established method was extensively validated by determining the linearity (R2 > 0.999), sensitivity (limit of quantitation, 0.16 mg/100 g), recovery (83.1-91.6%), precision (RSD < 5.4%) and repeatability (RSD < 7.7%). To validate the applicability of the method, four different brands of infant formulas in China were analysed. The acquired contents of bovine lactoferrin were 52.60-150.56 mg/100 g.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão
Fórmulas Infantis/análise
Lactoferrina/análise
Peptídeos/análise
Espectrometria de Massas em Tandem
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Calibragem
Isótopos de Carbono/química
Bovinos
Seres Humanos
Lactente
Fórmulas Infantis/normas
Marcação por Isótopo
Lactoferrina/metabolismo
Lactoferrina/normas
Isótopos de Nitrogênio/química
Peptídeos/química
Peptídeos/normas
Padrões de Referência
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbon Isotopes); 0 (Nitrogen Isotopes); 0 (Peptides); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184152


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[PMID]:28867180
[Au] Autor:Aizawa S; Hoki M; Yamamuro Y
[Ad] Endereço:Laboratory of Animal Genetics and Physiology, Department of Animal Science, College of Bioresource Sciences, Nihon University, Japan. Electronic address: aizawa.shu@nihon-u.ac.jp.
[Ti] Título:Lactoferrin promotes autophagy via AMP-activated protein kinase activation through low-density lipoprotein receptor-related protein 1.
[So] Source:Biochem Biophys Res Commun;493(1):509-513, 2017 Nov 04.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lactoferrin (LF) is a multifunctional, iron-binding glycoprotein in mammalian secretions, such as breast milk, and has several beneficial effects for human health. However, how these effects are exerted at the cellular level is still largely unknown. In this study, we investigated the effects of LF on autophagy activity in NIH/3T3 mouse fibroblasts. LF from bovine milk was found to increase LC3-I to LC3-II conversion and LC3-positive cytosolic punctate structures because of increased autophagy flux. Knockdown of the putative LF receptor low-density receptor-related protein 1 (LRP1) completely abolished LC3 conversion in cells by LF treatment. Moreover, exposure to LF increased the phosphorylation levels of AMPK in cells, and treatment of dorsomorphin, a pharmacological inhibitor of AMPK signaling, attenuated LC3 conversion by LF. Therefore, we concluded that the beneficial effects of LF might be due to an increase of autophagy activity via AMPK signaling through the LRP1 receptor. These findings provide a novel insight into the physiological role of LF for the maintenance of cellular and tissue homeostasis.
[Mh] Termos MeSH primário: Proteínas Quinases Ativadas por AMP/metabolismo
Autofagia/efeitos dos fármacos
Autofagia/fisiologia
Proteína Associada a Proteínas Relacionadas a Receptor de LDL/metabolismo
Lactoferrina/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Relação Dose-Resposta a Droga
Ativação Enzimática/efeitos dos fármacos
Camundongos
Células NIH 3T3
Fosforilação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (LDL-Receptor Related Protein-Associated Protein); EC 2.7.11.31 (AMP-Activated Protein Kinases); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE



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