Base de dados : MEDLINE Pesquisa : D08.811.277.656.300.760.560.500 [Categoria DeCS] Referências encontradas : 3526 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 353

ir para página

1 / 3526

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 29437053 [Au] Autor: Tarabichi M; Shohat N; Goswami K; Parvizi J [Ad] Endereço: The Rothman Institute at Thomas Jefferson University, 125 South 9th Street, Suite 1000, Philadelphia PA 19107, USA. [Ti] Título: Can next generation sequencing play a role in detecting pathogens in synovial fluid? [So] Source: Bone Joint J;100-B(2):127-133, 2018 02. [Is] ISSN: 2049-4408 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: AIMS: The diagnosis of periprosthetic joint infection can be difficult due to the high rate of culture-negative infections. The aim of this study was to assess the use of next-generation sequencing for detecting organisms in synovial fluid. MATERIALS AND METHODS: In this prospective, single-blinded study, 86 anonymized samples of synovial fluid were obtained from patients undergoing aspiration of the hip or knee as part of the investigation of a periprosthetic infection. A panel of synovial fluid tests, including levels of C-reactive protein, human neutrophil elastase, total neutrophil count, alpha-defensin, and culture were performed prior to next-generation sequencing. RESULTS: Of these 86 samples, 30 were alpha-defensin-positive and culture-positive (Group I), 24 were alpha-defensin-positive and culture-negative (Group II) and 32 were alpha-defensin-negative and culture-negative (Group III). Next-generation sequencing was concordant with 25 results for Group I. In four of these, it detected antibiotic resistant bacteria whereas culture did not. In another four samples with relatively low levels of inflammatory biomarkers, culture was positive but next-generation sequencing was negative. A total of ten samples had a positive next-generation sequencing result and a negative culture. In five of these, alpha-defensin was positive and the levels of inflammatory markers were high. In the other five, alpha-defensin was negative and the levels of inflammatory markers were low. While next-generation sequencing detected several organisms in each sample, in most samples with a higher probability of infection, there was a predominant organism present, while in those presumed not to be infected, many organisms were identified with no predominant organism. CONCLUSION: Pathogens causing periprosthetic infection in both culture-positive and culture-negative samples of synovial fluid could be identified by next-generation sequencing. Cite this article: 2018;100-B:127-33. [Mh] Termos MeSH primário: Artroplastia de Quadril Artroplastia do Joelho Infecções Bacterianas/microbiologia Micoses/microbiologia Infecções Relacionadas à Prótese/microbiologia Análise de Sequência de DNA/métodos Líquido Sinovial/química Líquido Sinovial/microbiologia [Mh] Termos MeSH secundário: Biomarcadores/análise Proteína C-Reativa/análise Seres Humanos Elastase de Leucócito/análise Reação em Cadeia da Polimerase Estudos Prospectivos Sensibilidade e Especificidade Método Simples-Cego alfa-Defensinas/análise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biomarkers); 0 (alpha-Defensins); 9007-41-4 (C-Reactive Protein); EC 3.4.21.37 (Leukocyte Elastase) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180216 [Lr] Data última revisão: 180216 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 180214 [St] Status: MEDLINE [do] DOI: 10.1302/0301-620X.100B2.BJJ-2017-0531.R2

2 / 3526

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28468826 [Au] Autor: Kerros C; Tripathi SC; Zha D; Mehrens JM; Sergeeva A; Philips AV; Qiao N; Peters HL; Katayama H; Sukhumalchandra P; Ruisaard KE; Perakis AA; St John LS; Lu S; Mittendorf EA; Clise-Dwyer K; Herrmann AC; Alatrash G; Toniatti C; Hanash SM; Ma Q; Molldrem JJ [Ad] Endereço: From the University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030. [Ti] Título: Neuropilin-1 mediates neutrophil elastase uptake and cross-presentation in breast cancer cells. [So] Source: J Biol Chem;292(24):10295-10305, 2017 06 16. [Is] ISSN: 1083-351X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Neutrophil elastase (NE) can be rapidly taken up by tumor cells that lack endogenous NE expression, including breast cancer, which results in cross-presentation of PR1, an NE-derived HLA-A2-restricted peptide that is an immunotherapy target in hematological and solid tumor malignancies. The mechanism of NE uptake, however, remains unknown. Using the mass spectrometry-based approach, we identify neuropilin-1 (NRP1) as a NE receptor that mediates uptake and PR1 cross-presentation in breast cancer cells. We demonstrated that soluble NE is a specific, high-affinity ligand for NRP1 with a calculated of 38.7 nm Furthermore, we showed that NRP1 binds to the RR R motif in NE. Notably, NRP1 knockdown with interfering RNA or CRISPR-cas9 system and blocking using anti-NRP1 antibody decreased NE uptake and, subsequently, susceptibility to lysis by PR1-specific cytotoxic T cells. Expression of NRP1 in NRP1-deficient cells was sufficient to induce NE uptake. Altogether, because NRP1 is broadly expressed in tumors, our findings suggest a role for this receptor in immunotherapy strategies that target cross-presented antigens. [Mh] Termos MeSH primário: Absorção Fisiológica Neoplasias da Mama/metabolismo Apresentação Cruzada Elastase de Leucócito/metabolismo Proteínas de Neoplasias/metabolismo Neuropilina-1/metabolismo [Mh] Termos MeSH secundário: Motivos de Aminoácidos Anticorpos Bloqueadores/metabolismo Neoplasias da Mama/imunologia Neoplasias da Mama/patologia Sistemas CRISPR-Cas Linhagem Celular Tumoral Feminino Seres Humanos Cinética Elastase de Leucócito/química Elastase de Leucócito/imunologia Ligantes Proteínas de Neoplasias/antagonistas & inibidores Proteínas de Neoplasias/química Proteínas de Neoplasias/genética Neuropilina-1/antagonistas & inibidores Neuropilina-1/química Neuropilina-1/genética Fragmentos de Peptídeos/química Fragmentos de Peptídeos/genética Fragmentos de Peptídeos/metabolismo Domínios e Motivos de Interação entre Proteínas Interferência de RNA Proteínas Recombinantes/química Proteínas Recombinantes/metabolismo Solubilidade Linfócitos T Citotóxicos/imunologia Linfócitos T Citotóxicos/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL [Nm] Nome de substância: 0 (Antibodies, Blocking); 0 (Ligands); 0 (Neoplasm Proteins); 0 (Peptide Fragments); 0 (Recombinant Proteins); 144713-63-3 (Neuropilin-1); EC 3.4.21.37 (Leukocyte Elastase) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 171228 [Lr] Data última revisão: 171228 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170505 [St] Status: MEDLINE [do] DOI: 10.1074/jbc.M116.773051

3 / 3526

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28922398 [Au] Autor: Chung HS; Kim JS; Lee SM; Park SJ [Ad] Endereço: Alteogen Inc., Yuseong-gu, Daejeon, South Korea. [Ti] Título: Role of the P2 residue of human alpha 1-antitrypsin in determining target protease specificity. [So] Source: PLoS One;12(9):e0185074, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Alpha 1-antitrypsin (A1AT) is a serine protease inhibitor that mainly inhibits neutrophil elastase in the lungs. A variant of A1AT at the P1 position with methionine 358 to arginine (A1AT-Pittsburgh) is a rapid inhibitor of thrombin with greatly diminished anti-elastase activity. The P2 residue (position 357) of A1AT-Pittsburgh has been shown to play an important role in interactions with thrombin and kallikrein, but the role of P2 residue in wild-type A1AT has largely been unraveled. Here, we investigated the effects of P2 proline substitutions in wild-type A1AT on interactions with porcine pancreatic elastase (PPE) and human neutrophil elastase (HNE). The mutant A1AT proteins (P357A, P357D, P357K, P357L, P357N, P357S, and P357W) were less efficient than the wild-type A1AT at inhibiting PPE and HNE. Among the mutants, P357D did not form a complex with PPE, whereas P357L, P357N, and P357W showed significantly reduced complex formation with PPE. Surprisingly, mass spectrometry analysis revealed that P357D had two cleavage sites after the P9 alanine and the P3 isoleucine residues. Our results indicate that the size and negative charge of the R group of the P2 residue influence the interaction with elastases. Specifically, the negative charge at the P2 residue is disfavored and the resulting conformational changes in the reactive center loop upon interaction with PPE lead to cleavage at new sites. Overall, the results of this study demonstrate a previously unknown role for P2 residue in determining inhibitory specificity of A1AT. [Mh] Termos MeSH primário: Elastase de Leucócito/química alfa 1-Antitripsina/química [Mh] Termos MeSH secundário: Substituição de Aminoácidos Animais Seres Humanos Elastase de Leucócito/metabolismo Mutação de Sentido Incorreto Suínos alfa 1-Antitripsina/genética alfa 1-Antitripsina/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (SERPINA1 protein, human); 0 (alpha 1-Antitrypsin); EC 3.4.21.37 (Leukocyte Elastase) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171013 [Lr] Data última revisão: 171013 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170919 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0185074

4 / 3526

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28800474 [Au] Autor: Lee JW; Park HA; Kwon OK; Park JW; Lee G; Lee HJ; Lee SJ; Oh SR; Ahn KS [Ad] Endereço: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk, 363-883, Republic of Korea. [Ti] Título: NPS 2143, a selective calcium-sensing receptor antagonist inhibits lipopolysaccharide-induced pulmonary inflammation. [So] Source: Mol Immunol;90:150-157, 2017 Oct. [Is] ISSN: 1872-9142 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: NPS 2143, a novel and selective antagonist of calcium-sensing receptor (CaSR) has been reported to possess anti-inflammatory activity. In the present study, we examined the protective effect of NPS 2143 on lipopolysaccharide (LPS)-induced acute lung injury (ALI). NPS 2143 pretreatment significantly inhibited the influx of inflammatory cells and the expression of monocyte chemoattractant protein-1 (MCP-1) in the lung of mice with LPS-induced ALI. NPS 2143 decreased the levels of neutrophil elastase (NE) and protein concentration in the bronchoalveolar lavage fluid (BALF). NPS 2143 also reduced the production of inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the BALF and serum. In addition, NPS 2143 attenuated the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and increased the activation of AMP-activated protein kinase (AMPK) in the lung. NPS 2143 also downregulated the activation of nuclear factor-kappa B (NF-κB) in the lung. In LPS-stimulated H292 airway epithelial cells, NPS 2143 attenuated the releases of IL-6 and MCP-1. Furthermore, NPS 2143 upregulated the activation of AMPK and downregulated the activation of NF-κB. These results suggest that NPS 2143 could be potential agent for the treatment of inflammatory diseases including ALI. [Mh] Termos MeSH primário: Lesão Pulmonar Aguda/prevenção & controle Anti-Inflamatórios/farmacologia Naftalenos/farmacologia Pneumonia/prevenção & controle Receptores de Detecção de Cálcio/antagonistas & inibidores Mucosa Respiratória/efeitos dos fármacos [Mh] Termos MeSH secundário: Proteínas Quinases Ativadas por AMP/metabolismo Lesão Pulmonar Aguda/induzido quimicamente Animais Líquido da Lavagem Broncoalveolar/química Linhagem Celular Quimiocina CCL2/biossíntese Ciclo-Oxigenase 2/biossíntese Ativação Enzimática/efeitos dos fármacos Células Epiteliais/efeitos dos fármacos Seres Humanos Interleucina-6/metabolismo Elastase de Leucócito/metabolismo Lipopolissacarídeos Pulmão/patologia Masculino Camundongos Camundongos Endogâmicos C57BL NF-kappa B/biossíntese Óxido Nítrico Sintase Tipo II/biossíntese Pneumonia/induzido quimicamente Mucosa Respiratória/citologia Fator de Necrose Tumoral alfa/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents); 0 (Ccl2 protein, mouse); 0 (Chemokine CCL2); 0 (Interleukin-6); 0 (Lipopolysaccharides); 0 (N-(2-hydroxy-3-(2-cyano-3-chlorophenoxy)propyl)-1,1-dimethyl-2-(2-nephthyl)ethylamine); 0 (NF-kappa B); 0 (Naphthalenes); 0 (Receptors, Calcium-Sensing); 0 (Tumor Necrosis Factor-alpha); 0 (interleukin-6, mouse); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.99.- (Ptgs2 protein, mouse); EC 1.14.99.1 (Cyclooxygenase 2); EC 2.7.11.31 (AMP-Activated Protein Kinases); EC 3.4.21.37 (Leukocyte Elastase) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170812 [St] Status: MEDLINE

5 / 3526

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28796788 [Au] Autor: Cruz-Baquero A; Cárdenas Jaramillo LM; Gutiérrez-Meza M; Jarillo-Luna RA; Campos-Rodríguez R; Rivera-Aguilar V; Miliar-García A; Pacheco-Yepez J [Ad] Endereço: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP, Ciudad de México, México. [Ti] Título: Different behavior of myeloperoxidase in two rodent amoebic liver abscess models. [So] Source: PLoS One;12(8):e0182480, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The protozoan Entamoeba histolytica is the etiological agent of amoebiasis, which can spread to the liver and form amoebic liver abscesses. Histological studies conducted with resistant and susceptible models of amoebic liver abscesses (ALAs) have established that neutrophils are the first cells to contact invasive amoebae at the lesion site. Myeloperoxidase is the most abundant enzyme secreted by neutrophils. It uses hydrogen peroxide secreted by the same cells to oxidize chloride ions and produce hypochlorous acid, which is the most efficient microbicidal system of neutrophils. In a previous report, our group demonstrated that myeloperoxidase presents amoebicidal activity in vitro. The aim of the current contribution was to analyze in vivo the role of myeloperoxidase in a susceptible (hamsters) and resistant (Balb/c mice) animal models of ALAs. In liver samples of hamsters and mice inoculated intraportally with Entamoeba histolytica trophozoites, the number of neutrophils in ALAs was determined by enzymatic activity. The presence of myeloperoxidase was observed by staining, and its expression and activity were quantified in situ. A significant difference existed between the two animal models in the number of neutrophils and the expression and activity of myeloperoxidase, which may explain the distinct evolution of amoebic liver abscesses. Hamsters and mice were treated with an MPO inhibitor (4-aminobenzoic acid hydrazide). Hamsters treated with ABAH showed no significant differences in the percentage of lesions or in the percentage of amoebae damaged compared with the untreated hamsters. ABAH treated mice versus untreated mice showed larger abscesses and a decreased percentage of damaged amoebae in these lesion at all stages of evolution. Further studies are needed to elucidate the host and amoebic mechanisms involved in the adequate or inadequate activation and modulation of myeloperoxidase. [Mh] Termos MeSH primário: Entamoeba histolytica/fisiologia Abscesso Hepático Amebiano/enzimologia Peroxidase/metabolismo [Mh] Termos MeSH secundário: Animais Cricetinae Modelos Animais de Doenças Resistência à Doença Interações Hospedeiro-Patógeno Elastase de Leucócito/metabolismo Fígado/enzimologia Fígado/imunologia Fígado/parasitologia Masculino Camundongos Endogâmicos BALB C Neutrófilos/enzimologia [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: EC 1.11.1.7 (Peroxidase); EC 3.4.21.37 (Leukocyte Elastase) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171006 [Lr] Data última revisão: 171006 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170811 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0182480

6 / 3526

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28754797 [Au] Autor: Makaryan V; Kelley ML; Fletcher B; Bolyard AA; Aprikyan AA; Dale DC [Ad] Endereço: Department of Medicine, University of Washington, Seattle, Washington, USA. [Ti] Título: Elastase inhibitors as potential therapies for -associated neutropenia. [So] Source: J Leukoc Biol;102(4):1143-1151, 2017 Oct. [Is] ISSN: 1938-3673 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Mutations in , the gene for neutrophil elastase (NE), a protease expressed early in neutrophil development, are the most frequent cause of cyclic (CyN) and severe congenital neutropenia (SCN). We hypothesized that inhibitors of NE, acting either by directly inhibiting enzymatic activity or as chaperones for the mutant protein, might be effective as therapy for CyN and SCN. We investigated ß-lactam-based inhibitors of human NE (Merck Research Laboratories, Kenilworth, NJ, USA), focusing on 1 inhibitor called MK0339, a potent, orally absorbed agent that had been tested in clinical trials and shown to have a favorable safety profile. Because fresh, primary bone marrow cells are rarely available in sufficient quantities for research studies, we used 3 cellular models: patient-derived, induced pluripotent stem cells (iPSCs); HL60 cells transiently expressing mutant NE; and HL60 cells with regulated expression of the mutant enzyme. In all 3 models, the cells expressing the mutant enzyme had reduced survival as measured with annexin V and FACS. Coincubation with the inhibitors, particularly MK0339, promoted cell survival and increased formation of mature neutrophils. These studies suggest that cell-permeable inhibitors of neutrophil elastase show promise as novel therapies for -associated neutropenia. [Mh] Termos MeSH primário: Inibidores Enzimáticos/farmacologia Elastase de Leucócito Mutação Neutropenia/congênito [Mh] Termos MeSH secundário: Sobrevivência Celular Feminino Células HL-60 Seres Humanos Elastase de Leucócito/antagonistas & inibidores Elastase de Leucócito/genética Elastase de Leucócito/metabolismo Masculino Neutropenia/tratamento farmacológico Neutropenia/enzimologia Neutropenia/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Enzyme Inhibitors); EC 3.4.21.37 (Leukocyte Elastase) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171009 [Lr] Data última revisão: 171009 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170730 [St] Status: MEDLINE [do] DOI: 10.1189/jlb.5A1016-445R

7 / 3526

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28704150 [Au] Autor: Power G; Moore Z; O'Connor T [Ad] Endereço: Community Registered General Nurse, HSE Carlow/Kilkenny, Ireland. [Ti] Título: Measurement of pH, exudate composition and temperature in wound healing: a systematic review. [So] Source: J Wound Care;26(7):381-397, 2017 Jul 02. [Is] ISSN: 0969-0700 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: OBJECTIVE: To assess the potential of measurements of pH, exudate composition and temperature in wounds to predict healing outcomes and to identify the methods that are employed to measure them. METHOD: A systematic review based on the outcomes of a search strategy of quantitative primary research published in the English language was conducted. Inclusion criteria limited studies to those involving in vivo and human participants with an existing or intentionally provoked wound, defined as 'a break in the epithelial integrity of the skin', and excluded in vitro and animal studies. Data synthesis and analysis was performed using structured narrative summaries of each included study arranged by concept, pH, exudate composition and temperature. The Evidence Based Literature (EBL) Critical Appraisal Checklist was implemented to appraise the quality of the included studies. RESULTS: A total of 23 studies, three for pH (mean quality score 54.48%), 12 for exudate composition (mean quality score 46.54%) and eight for temperature (mean quality score 36.66%), were assessed as eligible for inclusion in this review. Findings suggest that reduced pH levels in wounds, from alkaline towards acidic, are associated with improvements in wound condition. Metalloproteinase-9 (MMP-9), matrix metalloproteinase-2 (MMP-2), tissue inhibitor of metalloproteinase (TIMP), neutrophil elastase (NE) and albumin, in descending order, were the most frequently measured analytes in wounds. MMP-9 emerged as the analyte which offers the most potential as a biomarker of wound healing, with elevated levels observed in acute or non-healing wounds and decreasing levels in wounds progressing in healing. Combined measures of different exudate components, such as MMP/TIMP ratios, also appeared to offer substantial potential to indicate wound healing. Finally, temperature measurements are highest in non-healing, worsening or acute wounds and decrease as wounds progress towards healing. Methods used to measure pH, exudate composition and temperature varied greatly and, despite noting some similarities, the studies often yielded significantly contrasting results. Furthermore, a limitation to the generalisability of the findings was the overall quality scores of the research studies, which appeared suboptimal. CONCLUSION: Despite some promising findings, there was insufficient evidence to confidently recommend the use of any of these measures as predictors of wound healing. pH measurement appeared as the most practical method for use in clinical practice to indicate wound healing outcomes. Further research is required to increase the strength of evidence and develop a greater understanding of wound healing dynamics. [Mh] Termos MeSH primário: Biomarcadores/metabolismo Exsudatos e Transudatos/metabolismo Temperatura Cutânea Cicatrização Ferimentos e Lesões [Mh] Termos MeSH secundário: Albuminas/metabolismo Exsudatos e Transudatos/química Seres Humanos Concentração de Íons de Hidrogênio Elastase de Leucócito/metabolismo Metaloproteinase 2 da Matriz/metabolismo Metaloproteinase 9 da Matriz/metabolismo Temperatura Ambiente Inibidores Teciduais de Metaloproteinases/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Albumins); 0 (Biomarkers); 0 (Tissue Inhibitor of Metalloproteinases); EC 3.4.21.37 (Leukocyte Elastase); EC 3.4.24.24 (MMP2 protein, human); EC 3.4.24.24 (Matrix Metalloproteinase 2); EC 3.4.24.35 (MMP9 protein, human); EC 3.4.24.35 (Matrix Metalloproteinase 9) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170926 [Lr] Data última revisão: 170926 [Sb] Subgrupo de revista: N [Da] Data de entrada para processamento: 170714 [St] Status: MEDLINE [do] DOI: 10.12968/jowc.2017.26.7.381

8 / 3526

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28696508 [Au] Autor: Laridan E; Denorme F; Desender L; François O; Andersson T; Deckmyn H; Vanhoorelbeke K; De Meyer SF [Ad] Endereço: Laboratory for Thrombosis Research, KU Leuven, Campus Kulak Kortrijk, Kortrijk, Belgium. [Ti] Título: Neutrophil extracellular traps in ischemic stroke thrombi. [So] Source: Ann Neurol;82(2):223-232, 2017 Aug. [Is] ISSN: 1531-8249 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: OBJECTIVE: Neutrophil extracellular traps (NETs) have been shown to promote thrombus formation. Little is known about the exact composition of thrombi that cause ischemic stroke. In particular, no information is yet available on the presence of NETs in cerebral occlusions. Such information is, however, essential to improve current thrombolytic therapy with tissue plasminogen activator (t-PA). This study aimed at investigating the presence of neutrophils and more specifically NETs in ischemic stroke thrombi. METHODS: Sixty-eight thrombi retrieved from ischemic stroke patients undergoing endovascular treatment were characterized by immunostaining using neutrophil markers (CD66b and neutrophil elastase) and NET markers (citrullinated histone H3 [H3Cit] and extracellular DNA). Neutrophils and NETs were quantified. In addition, extracellular DNA was targeted by performing ex vivo lysis of retrieved thrombi with DNase 1 and t-PA. RESULTS: Neutrophils were detected extensively throughout all thrombi. H3Cit, a hallmark of NETs, was observed in almost all thrombi. H3Cit-positive area varied up to 13.45% of total thrombus area. Colocalization of H3Cit with extracellular DNA released from neutrophils confirmed the specific presence of NETs. H3Cit was more abundant in thrombi of cardioembolic origin compared to other etiologies. Older thrombi contained significantly more neutrophils and H3Cit compared to fresh thrombi. Interestingly, ex vivo lysis of patient thrombi was more successful when adding DNase 1 to standard t-PA. INTERPRETATION: Neutrophils and NETs form important constituents of cerebral thrombi. Targeting of NETs with DNase 1 might have prothrombolytic potential in treatment of acute ischemic stroke. Ann Neurol 2017;82:223-232. [Mh] Termos MeSH primário: Antígenos CD/metabolismo Isquemia Encefálica/metabolismo Moléculas de Adesão Celular/metabolismo DNA/metabolismo Armadilhas Extracelulares/metabolismo Elastase de Leucócito/metabolismo Acidente Vascular Cerebral/metabolismo Trombose/metabolismo [Mh] Termos MeSH secundário: Adolescente Adulto Idoso Idoso de 80 Anos ou mais Biomarcadores Isquemia Encefálica/complicações Contagem de Células/estatística & dados numéricos Feminino Proteínas Ligadas por GPI/metabolismo Seres Humanos Masculino Meia-Idade Acidente Vascular Cerebral/complicações Adulto Jovem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antigens, CD); 0 (Biomarkers); 0 (CEACAM8 protein, human); 0 (Cell Adhesion Molecules); 0 (GPI-Linked Proteins); 9007-49-2 (DNA); EC 3.4.21.37 (Leukocyte Elastase) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170926 [Lr] Data última revisão: 170926 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170712 [St] Status: MEDLINE [do] DOI: 10.1002/ana.24993

9 / 3526

MEDLINE

seleciona para imprimir Fotocópia

[PMID]: 28685539 [Au] Autor: Liu L; Liu DZ; Wang QP; Zhu ZL; Li HM; Lu XY [Ad] Endereço: Xinxiang Central Hospital, Xinxiang City, China. [Ti] Título: Respiratory training during rehabilitation of acute organic fluorine-poisoned patients treated by non-invasive positive pressure ventilation. [So] Source: J Biol Regul Homeost Agents;31(2):371-376, 2017 Apr-Jun. [Is] ISSN: 0393-974X [Cp] País de publicação: Italy [La] Idioma: eng [Ab] Resumo: This paper aimed to analyze the effects of respiratory training on pulmonary function during the rehabilitation period for acute organic fluorine-poisoned patients treated by non-invasive positive pressure ventilation (NIPPV). Sixty-two acute organic fluorine-poisoned patients admitted to the Xinxiang Central Hospital, Xinxiang City, China, from May 2012 to March 2016 were selected and randomly divided into an observation group and a control group, with 31 cases in each. Both groups received NIPPV. The patients in the control group exercised daily, while the patients in the observation group received contracting lips-abdominal breathing training. The therapeutic effects, pulmonary ventilation function, serum levels of α-antitrypsin1 (α-AT1), surfactant protein D (SP-D), neutrophil elastase (NE), transforming growth factor beta 1 (TGF-ß1), and quality of life were analyzed and compared between the two groups both before and after the administration of treatment. The total effective rate of the observation group was 93.55%, which was significantly higher when compared with the control group (74.19%) (P less than 0.05). The levels of forced expiratory volume in one second (FEV1), FEV1/FVC ratio, vital capacity (VC), carbon monoxide diffusion capacity (DLco), and maximal voluntary ventilation (MVV) of the observation group were better when compared with the control group and had statistical significance (P less than 0.05). Before treatment, the serum levels of α-AT1, SP-D, NE, and TGF-ß1, and quality of life had no statistical significance in either group (P>0.05); after treatment, these indexes and the quality of life for the observation group were significantly higher when compared with the control group, with statistical significance (P less than 0.05). The respiratory training in acute organic fluorine-poisoned patients treated by NIPPV can improve the serum indexes, dilute toxicity, and recover pulmonary function, which play key roles in improving the therapeutic effects and quality of life of patients, and is worthy of clinical promotion. [Mh] Termos MeSH primário: Exercícios Respiratórios Hidrocarbonetos Fluorados/envenenamento Elastase de Leucócito/sangue Respiração com Pressão Positiva Proteína D Associada a Surfactante Pulmonar/sangue Fator de Crescimento Transformador beta1/sangue alfa 1-Antitripsina/sangue [Mh] Termos MeSH secundário: Adulto Feminino Seres Humanos Masculino Meia-Idade [Pt] Tipo de publicação: CLINICAL TRIAL; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Hydrocarbons, Fluorinated); 0 (Pulmonary Surfactant-Associated Protein D); 0 (TGFB1 protein, human); 0 (Transforming Growth Factor beta1); 0 (alpha 1-Antitrypsin); EC 3.4.21.37 (Leukocyte Elastase) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170814 [Lr] Data última revisão: 170814 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170708 [St] Status: MEDLINE

10 / 3526

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28612630 [Au] Autor: Vergelli C; Schepetkin IA; Crocetti L; Iacovone A; Giovannoni MP; Guerrini G; Khlebnikov AI; Ciattini S; Ciciani G; Quinn MT [Ad] Endereço: a NEUROFARBA, Sezione di Farmaceutica e Nutraceutica , Università degli Studi di Firenze , Sesto Fiorentino , Italy. [Ti] Título: Isoxazol-5(2H)-one: a new scaffold for potent human neutrophil elastase (HNE) inhibitors. [So] Source: J Enzyme Inhib Med Chem;32(1):821-831, 2017 Dec. [Is] ISSN: 1475-6374 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Human neutrophil elastase (HNE) is an important target for the development of novel and selective inhibitors to treat inflammatory diseases, especially pulmonary pathologies. Here, we report the synthesis, structure-activity relationship analysis, and biological evaluation of a new series of HNE inhibitors with an isoxazol-5(2H)-one scaffold. The most potent compound (2o) had a good balance between HNE inhibitory activity (IC value =20 nM) and chemical stability in aqueous buffer (t =8.9 h). Analysis of reaction kinetics revealed that the most potent isoxazolone derivatives were reversible competitive inhibitors of HNE. Furthermore, since compounds 2o and 2s contain two carbonyl groups (2-N-CO and 5-CO) as possible points of attack for Ser195, the amino acid of the active site responsible for the nucleophilic attack, docking studies allowed us to clarify the different roles played by these groups. [Mh] Termos MeSH primário: Isoxazóis/farmacologia Elastase de Leucócito/antagonistas & inibidores Inibidores de Serino Proteinase/farmacologia [Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga Seres Humanos Isoxazóis/síntese química Isoxazóis/química Elastase de Leucócito/metabolismo Estrutura Molecular Inibidores de Serino Proteinase/síntese química Inibidores de Serino Proteinase/química Relação Estrutura-Atividade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Isoxazoles); 0 (Serine Proteinase Inhibitors); EC 3.4.21.37 (Leukocyte Elastase) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170906 [Lr] Data última revisão: 170906 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170615 [St] Status: MEDLINE [do] DOI: 10.1080/14756366.2017.1326915

---

página 1 de 353

ir para página

Refinar a pesquisa

Base de dados : MEDLINE

Formulário avançado

		Pesquisar	no campo
1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde