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Pesquisa : D08.811.399.475.200.350 [Categoria DeCS]
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[PMID]:29236388
[Au] Autor:Minchenko OH; Garmash IA; Minchenko DO; Kuznetsova AY; Ratushna OO
[Ti] Título:Inhibition of IRE1 modifies hypoxic regulation of G6PD, GPI, TKT, TALDO1, PGLS and RPIA genes expression in U87 glioma cells.
[So] Source:Ukr Biochem J;89(1):38-49, 2017 Jan-Feb.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:We have studied the effect of hypoxia on the expression level of mRNA of the basic enzymes of pentose-phosphate cycle (G6PD, TKT, TALDO1, PGLS and RPIA) and glucose-6-phosphate isomerase (GPI) in U87 glioma cells in relation to inhibition of IRE1 (inositol requiring enzyme 1). It was shown that hypoxia leads to up-regulation of the expression of GPI and PGLS genes and to down-regulation of TALDO1 and RPIA genes in control glioma cells. Changes for GPI gene were more significant than for other genes. At the same time, inhibition of IRE1 modified the effect of hypoxia on the expression of all studied genes. In particular, it increased sensitivity to hypoxia of G6PD and TKT genes expression and suppressed the effect of hypoxia on the expression of GPI and RPIA genes. Additionally, inhibition of IRE1 eliminated hypoxic regulation of PGLS gene and did not change significantly effect of hypoxia on the expression of TALDO1 gene in glioma cells. Present study demonstrated that hypoxia, which often contributes to tumor growth, affects the expression of most studied genes and inhibition of IRE1 modified the hypoxic regulation of pentose-phosphate cycle gene expressions in a gene specific manner and thus possibly contributes to slower glioma growth, but several aspects of this regulation warrant further investigation.
[Mh] Termos MeSH primário: Estresse do Retículo Endoplasmático/genética
Endorribonucleases/genética
Regulação Neoplásica da Expressão Gênica
Neuroglia/metabolismo
Via de Pentose Fosfato/genética
Proteínas Serina-Treonina Quinases/genética
[Mh] Termos MeSH secundário: Aldose-Cetose Isomerases/genética
Aldose-Cetose Isomerases/metabolismo
Hidrolases de Éster Carboxílico/genética
Hidrolases de Éster Carboxílico/metabolismo
Hipóxia Celular
Linhagem Celular Tumoral
Movimento Celular
Proliferação Celular
Endorribonucleases/deficiência
Técnicas de Silenciamento de Genes
Glucose-6-Fosfato Isomerase/genética
Glucose-6-Fosfato Isomerase/metabolismo
Glucosefosfato Desidrogenase/genética
Glucosefosfato Desidrogenase/metabolismo
Seres Humanos
Neuroglia/patologia
Proteínas Serina-Treonina Quinases/deficiência
Transdução de Sinais
Transaldolase/genética
Transaldolase/metabolismo
Transcetolase/genética
Transcetolase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.1.1.49 (Glucosephosphate Dehydrogenase); EC 2.2.1.1 (Transketolase); EC 2.2.1.2 (Transaldolase); EC 2.7.11.1 (ERN1 protein, human); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 3.1.- (Endoribonucleases); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.31 (6-phosphogluconolactonase); EC 5.3.1.- (Aldose-Ketose Isomerases); EC 5.3.1.6 (ribosephosphate isomerase); EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj89.01.038


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[PMID]:28539551
[Au] Autor:Hirota T; Tsuboi H; Takahashi H; Asashima H; Ohta M; Wakasa Y; Matsumoto I; Takaiwa F; Sumida T
[Ad] Endereço:Department of Internal Medicine, Faculty of Medicine, University of Tsukuba.
[Ti] Título:Suppression of GPI-induced arthritis by oral administration of transgenic rice seeds expressing altered peptide ligands.
[So] Source:Nihon Rinsho Meneki Gakkai Kaishi;40(1):28-34, 2017.
[Is] ISSN:1349-7413
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:OBJECTIVE: To investigate the effects and mechanisms of transgenic rice seeds expressing the altered peptide ligand (APL) of human glucose-6-phosphate-isomerase (hGPI ) in mice model of GPI induced arthritis (GIA). METHODS: We generated transgenic rice expressing APL12 which was analog peptide of hGPI . The transgenic rice seeds were orally administered prophylactically before the induction of GIA. The severity of arthritis and titers of serum anti-GPI antibodies were evaluated. We examined IL-17 production from splenocytes and inguinal lymph node (iLN) and mesenteric lymph nodes (mLN) cells and analyzed the expression levels of functional molecules from splenocytes and iLN cells. RESULTS: Prophylactic treatment of GIA mice with APL12 transgenic rice seeds (APL12-TG) significantly improved the severity of arthritis, histopathological arthritis scores, and decreased titers of serum anti-GPI antibodies, BAFF mRNA in iLN cells, IL-17 production in splenocytes and iLN cells compared with non-transgenic rice-treated mice. APL12-TG-treated GIA mice showed upregulation of Foxp3 and GITR protein in CD4 CD25 cells in the spleen. CONCLUSION: APL12-TG improved the severity of GIA through a decrease in production of IL-17 and anti-GPI antibodies via upregulation of Foxp3 and GITR expression on regulatory T cells in spleen.
[Mh] Termos MeSH primário: Artrite Reumatoide/imunologia
Artrite Reumatoide/prevenção & controle
Glucose-6-Fosfato Isomerase/imunologia
Oryza/genética
Peptídeos/administração & dosagem
Plantas Geneticamente Modificadas
Sementes
[Mh] Termos MeSH secundário: Administração Oral
Sequência de Aminoácidos
Animais
Anticorpos/sangue
Artrite Reumatoide/terapia
Modelos Animais de Doenças
Interleucina-17/sangue
Ligantes
Camundongos Endogâmicos DBA
Peptídeos/química
Fitoterapia
Índice de Gravidade de Doença
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antibodies); 0 (Interleukin-17); 0 (Ligands); 0 (Peptides); EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.2177/jsci.40.28


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[PMID]:28529468
[Au] Autor:Li Y; Hansson B; Ghatnekar L; Prentice HC
[Ad] Endereço:Department of Biology, Lund University, Lund, Sweden.
[Ti] Título:Contrasting patterns of nucleotide polymorphism suggest different selective regimes within different parts of the gene in L.
[So] Source:Hereditas;154:11, 2017.
[Is] ISSN:1601-5223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Phosphoglucose isomerase (PGI, EC 5.3.1.9) is an essential metabolic enzyme in all eukaryotes. An earlier study of the gene, which encodes cytosolic PGI in the grass L., revealed a marked difference in the levels of nucleotide polymorphism between the 5' and 3' portions of the gene. METHODS: In the present study, we characterized the sequence polymorphism in in more detail and examined possible explanations for the non-uniform pattern of nucleotide polymorphism across the gene. RESULTS: Our study confirms that the two portions of the gene show substantially different levels of DNA polymorphism and also suggests that the peptide encoded by the 3' portion of is functionally and structurally more important than that encoded by the 5' portion. Although there was some evidence of purifying selection ( / test) on the 5' portion of the gene, the signature of purifying selection was considerably stronger on the 3' portion of the gene ( / and McDonald-Kreitman tests). Several tests support the action of balancing selection within the 5' portion of the gene. Wall's and tests were significant only for the 5' portion of the gene. There were also marked peaks of nucleotide diversity, Tajima's and the / ratio at or around a codon site (within the 5' portion of the gene) that a previous study had suggested was subject to positive diversifying selection. CONCLUSIONS: Our results suggest that the two portions of the gene have been subject to different selective regimes. Purifying selection appears to have been the main force contributing to the relatively low level of polymorphism within the 3' portion of the sequence. In contrast, it is possible that balancing selection has contributed to the maintenance of the polymorphism within the 5' portion of the gene.
[Mh] Termos MeSH primário: Festuca/genética
Genes de Plantas
Glucose-6-Fosfato Isomerase/genética
Polimorfismo Genético
Seleção Genética
[Mh] Termos MeSH secundário: Sequência Conservada
DNA de Plantas/genética
Evolução Molecular
Festuca/enzimologia
Desequilíbrio de Ligação
Recombinação Genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE
[do] DOI:10.1186/s41065-017-0032-6


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[PMID]:28506033
[Au] Autor:He D; Pengtao G; Ju Y; Jianhua L; He L; Guocai Z; Xichen Z
[Ad] Endereço:College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
[Ti] Título:Differential Protein Expressions in Virus-Infected and Uninfected .
[So] Source:Korean J Parasitol;55(2):121-128, 2017 Apr.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Protozoan viruses may influence the function and pathogenicity of the protozoa. is a parasitic protozoan that could contain a double stranded RNA (dsRNA) virus, virus (TVV). However, there are few reports on the properties of the virus. To further determine variations in protein expression of , we detected 2 strains of ; the virus-infected (V ) and uninfected (V ) isolates to examine differentially expressed proteins upon TVV infection. Using a stable isotope N-terminal labeling strategy (iTRAQ) on soluble fractions to analyze proteomes, we identified 293 proteins, of which 50 were altered in V compared with V isolates. The results showed that the expression of 29 proteins was increased, and 21 proteins decreased in V isolates. These differentially expressed proteins can be classified into 4 categories: ribosomal proteins, metabolic enzymes, heat shock proteins, and putative uncharacterized proteins. Quantitative PCR was used to detect 4 metabolic processes proteins: glycogen phosphorylase, malate dehydrogenase, triosephosphate isomerase, and glucose-6-phosphate isomerase, which were differentially expressed in V and V isolates. Our findings suggest that mRNA levels of these genes were consistent with protein expression levels. This study was the first which analyzed protein expression variations upon TVV infection. These observations will provide a basis for future studies concerning the possible roles of these proteins in host-parasite interactions.
[Mh] Termos MeSH primário: Expressão Gênica
Proteínas de Protozoários/genética
Vírus de RNA
Trichomonas vaginalis/genética
Trichomonas vaginalis/virologia
[Mh] Termos MeSH secundário: Feminino
Glucose-6-Fosfato Isomerase/análise
Glucose-6-Fosfato Isomerase/isolamento & purificação
Glicogênio Fosforilase/análise
Glicogênio Fosforilase/isolamento & purificação
Glicólise/genética
Seres Humanos
Malato Desidrogenase/análise
Malato Desidrogenase/isolamento & purificação
Masculino
Reação em Cadeia da Polimerase
Proteínas de Protozoários/análise
Proteínas de Protozoários/classificação
Proteínas de Protozoários/isolamento & purificação
RNA de Cadeia Dupla
RNA Mensageiro/análise
Tricomoníase/parasitologia
Trichomonas vaginalis/crescimento & desenvolvimento
Trichomonas vaginalis/metabolismo
Triose-Fosfato Isomerase/análise
Triose-Fosfato Isomerase/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protozoan Proteins); 0 (RNA, Double-Stranded); 0 (RNA, Messenger); EC 1.1.1.37 (Malate Dehydrogenase); EC 2.4.1.- (Glycogen Phosphorylase); EC 5.3.1.1 (Triose-Phosphate Isomerase); EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.2.121


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[PMID]:28411162
[Au] Autor:Lyons BM; McHenry MA; Barrington DS
[Ad] Endereço:Pringle Herbarium, Plant Biology Department, University of Vermont, Torrey Hall, 27 Colchester Ave, Burlington, VT 05405, USA.
[Ti] Título:Insights into evolution in Andean Polystichum (Dryopteridaceae) from expanded understanding of the cytosolic phosphoglucose isomerase gene.
[So] Source:Mol Phylogenet Evol;112:36-46, 2017 Jul.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cytosolic phosphoglucose isomerase (pgiC) is an enzyme essential to glycolysis found universally in eukaryotes, but broad understanding of variation in the gene coding for pgiC is lacking for ferns. We used a substantially expanded representation of the gene for Andean species of the fern genus Polystichum to characterize pgiC in ferns relative to angiosperms, insects, and an amoebozoan; assess the impact of selection versus neutral evolutionary processes on pgiC; and explore evolutionary relationships of selected Andean species. The dataset of complete sequences comprised nine accessions representing seven species and one hybrid from the Andes and Serra do Mar. The aligned sequences of the full data set comprised 3376 base pairs (70% of the entire gene) including 17 exons and 15 introns from two central areas of the gene. The exons are highly conserved relative to angiosperms and retain substantial homology to insect pgiC, but intron length and structure are unique to the ferns. Average intron size is similar to angiosperms; intron number and location in insects are unlike those of the plants we considered. The introns included an array of indels and, in intron 7, an extensive microsatellite array with potential utility in analyzing population-level histories. Bayesian and maximum-parsimony analysis of 129 variable nucleotides in the Andean polystichums revealed that 59 (1.7% of the 3376 total) were phylogenetically informative; most of these united sister accessions. The phylogenetic trees for the Andean polystichums were incongruent with previously published cpDNA trees for the same taxa, likely the result of rapid evolutionary change in the introns and contrasting stability in the exons. The exons code a total of seven amino-acid substitutions. Comparison of non-synonymous to synonymous substitutions did not suggest that the pgiC gene is under selection in the Andes. Variation in pgiC including two additional accessions represented by incomplete sequences provided new insights into reticulate relationships among Andean taxa.
[Mh] Termos MeSH primário: Evolução Molecular
Glucose-6-Fosfato Isomerase/genética
Polystichum/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Teorema de Bayes
Citosol
DNA de Cloroplastos
Éxons
Íntrons
Filogenia
Polystichum/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast); EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE


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[PMID]:28126554
[Au] Autor:Liu F; Li S; Liu G; Li F
[Ad] Endereço:Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
[Ti] Título:Triosephosphate isomerase (TPI) facilitates the replication of WSSV in Exopalaemon carinicauda.
[So] Source:Dev Comp Immunol;71:28-36, 2017 06.
[Is] ISSN:1879-0089
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Triosephosphate isomerase (TPI) is a vital enzyme in the glycolytic pathway, which can catalyze the interconversion of glyceraldehyde-3-phosphate (GAP) and dihydroxyacetone phosphate (DHAP). DHAP is involved in lipid metabolism and phospholipid synthesis. In order to know the role of TPI in WSSV infection to prawn, we cloned the full length cDNA of triosephosphate isomerase gene (EcTPI) from Exopalaemon carinicauda, and its function during WSSV infection was analyzed. EcTPI transcripts were widely distributed in all tissues, but showed relatively higher expression levels in the gill and epidermis. Its expression was apparently up-regulated after 24 h post WSSV injection (hpi), when the virus load began to rise. Furthermore, we detected the expressions of the key genes encoding the enzymes which catalyze the key steps in the glycolysis during WSSV infection. The data showed that genes encoding the enzymes which catalyzed upper steps of glycolysis to produce GAP, including hexokinase (HK), glucose-6-phosphate isomerase (GPI) and phosphofructokinase-1 (PFK-1), were significantly up-regulated at 24 and 27 hpi. Genes encoding the enzymes catalyzing down steps of glycolysis after GAP, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), enolase (ENO) and pyruvate kinase (PK), were apparent down-regulated at 24 and 27 hpi. Meanwhile, the gene encoding the enzyme glycerol-3-phosphate dehydrogenase (GPDH) catalyzing DHAP to glycerol-3-phosphate (G-3-P) showed down-regulation at 12-27 hpi, while the gene encoding dihydroxyacetone-phosphate acyltransferase (DHAPAT) catalyzing DHAP to further synthesis of phospholipids showed up-regulation at 12-24 hpi. These data suggested that WSSV infection could change the glycolysis pathway to make them produce more phospholipids which could be very helpful for virus replication. In order to further confirm the above speculation, dsRNA interference (RNAi) approach was used to knock down EcTPI gene and analyze its effect on WSSV load in prawn. The data showed that interference of EcTPI gene led to a significant decrease of WSSV loads in WSSV infected prawn. These data provided useful information to understand the infection mechanism of WSSV.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/imunologia
Palaemonidae/imunologia
Triose-Fosfato Isomerase/metabolismo
Vírus 1 da Síndrome da Mancha Branca/fisiologia
[Mh] Termos MeSH secundário: Aciltransferases/metabolismo
Animais
Células Cultivadas
Regulação da Expressão Gênica
Inativação Gênica
Glucose-6-Fosfato Isomerase/metabolismo
Gliceraldeído 3-Fosfato/metabolismo
Glicólise/genética
RNA Interferente Pequeno/genética
Triose-Fosfato Isomerase/genética
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Small Interfering); 142-10-9 (Glyceraldehyde 3-Phosphate); EC 2.3.- (Acyltransferases); EC 2.3.1.42 (glycerone-phosphate O-acyltransferase); EC 5.3.1.1 (Triose-Phosphate Isomerase); EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE


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[PMID]:28062795
[Au] Autor:Clotet S; Soler MJ; Riera M; Pascual J; Fang F; Zhou J; Batruch I; Vasiliou SK; Dimitromanolakis A; Barrios C; Diamandis EP; Scholey JW; Konvalinka A
[Ad] Endereço:From the ‡Department of Nephrology, Hospital del Mar-Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain, 08003; sclotet88@gmail.com.
[Ti] Título:Stable Isotope Labeling with Amino Acids (SILAC)-Based Proteomics of Primary Human Kidney Cells Reveals a Novel Link between Male Sex Hormones and Impaired Energy Metabolism in Diabetic Kidney Disease.
[So] Source:Mol Cell Proteomics;16(3):368-385, 2017 Mar.
[Is] ISSN:1535-9484
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Male sex predisposes to many kidney diseases. Considering that androgens exert deleterious effects in a variety of cell types within the kidney, we hypothesized that dihydrotestosterone (DHT) would alter the biology of the renal tubular cell by inducing changes in the proteome. We employed stable isotope labeling with amino acids (SILAC) in an indirect spike-in fashion to accurately quantify the proteome in DHT- and 17ß-estradiol (EST)-treated human proximal tubular epithelial cells (PTEC). Of the 5043 quantified proteins, 76 were differentially regulated. Biological processes related to energy metabolism were significantly enriched among DHT-regulated proteins. SILAC ratios of 3 candidates representing glycolysis, N-acetylglucosamine metabolism and fatty acid ß-oxidation, namely glucose-6-phosphate isomerase (GPI), glucosamine-6-phosphate-N-acetyltransferase 1 (GNPNAT1), and mitochondrial trifunctional protein subunit alpha (HADHA), were verified , renal GPI and HADHA protein expression was significantly increased in males. Furthermore, male sex was associated with significantly higher GPI, GNPNAT1, and HADHA kidney protein expression in two different murine models of diabetes. Enrichment analysis revealed a link between our DHT-regulated proteins and oxidative stress within the diabetic kidney. This finding was validated , as we observed increased oxidative stress levels in control and diabetic male kidneys, compared with females. This in depth quantitative proteomics study of human primary PTEC response to sex hormone administration suggests that male sex hormone stimulation results in perturbed energy metabolism in kidney cells, and that this perturbation results in increased oxidative stress in the renal cortex. The proteome-level changes associated with androgens may play a crucial role in the development of structural and functional changes in the diseased kidney. With our findings, we propose a possible link between diabetic and non-diabetic kidney disease progression and male sex hormone levels. Data are available via ProteomeXchange (https://www.ebi.ac.uk/pride/archive/) with identifier PXD003811.
[Mh] Termos MeSH primário: Nefropatias Diabéticas/metabolismo
Di-Hidrotestosterona/farmacologia
Metabolismo Energético/efeitos dos fármacos
Rim/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Proteômica/métodos
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Citocinas/metabolismo
Modelos Animais de Doenças
Regulação da Expressão Gênica/efeitos dos fármacos
Glucose-6-Fosfato Isomerase/metabolismo
Seres Humanos
Marcação por Isótopo/métodos
Rim/citologia
Rim/metabolismo
Masculino
Camundongos
Subunidade alfa da Proteína Mitocondrial Trifuncional/metabolismo
Transferases (Outros Grupos de Fosfato Substituídos)/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 08J2K08A3Y (Dihydrotestosterone); EC 1.1.1.211 (HADHA protein, human); EC 1.1.1.211 (Mitochondrial Trifunctional Protein, alpha Subunit); EC 2.7.8.- (Transferases (Other Substituted Phosphate Groups)); EC 2.7.8.15 (GNPTAB protein, human); EC 5.3.1.9 (GPI protein, human); EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE
[do] DOI:10.1074/mcp.M116.061903


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[PMID]:27987494
[Au] Autor:Xu J; Liu J; Zhu L; Zhang XW; Li ZG
[Ad] Endereço:Department of Rheumatology and Immunology, Perking University People's Hospital, Key Laboratory for Rheumatism Mechanism and Immune Diagnosis, Peking-Tsinghua Center for Life Sciences, Beijing 100044, China; Department of Rheumatology and Immunology, Perking University International Hospital, Beijin
[Ti] Título:[Significance of glucose-6-phosphate isomerase assay in early diagnosis of rheumatoid arthritis].
[So] Source:Beijing Da Xue Xue Bao Yi Xue Ban;48(6):942-946, 2016 12 18.
[Is] ISSN:1671-167X
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To explore the titer of glucose-6-phosphate isomerase (GPI) for early diagnosis of the outpatient with rheumatoid arthritis (RA) in real life, and to analyze its relationship with disease activity. METHODS: In the study, 1 051 patients with arthritis were collected in the group who had joints tender and swelling, and 90 cases of healthy people as a control group. ELISA method was used to detect the serum level of GPI, and according to clinical features and laboratory test, all the patients including 525 RA patients, the other patients including osteoarthritis (OA), 134 cases of seronegative spine joint disease (SpA), 104 cases of systemic lupus erythematosus (SLE), 31 cases of primary Sjogren syndrome (pSS), 24 cases of gout arthritis (GA), 22 cases of other connective tissue diseases (including polymyalgia rheumatica, dermatomyositis, systemic sclerosis, adult Still disease) and 46 cases of other diseases (including 165 cases of osteoporosis, avascular necrosis of the femoral head, traumatic osteomyelitis, bone and joint disease, juvenile rheumatoid arthritis, tumor). The diagnostic values of GPI were assessed, and the differences between the GPI positive and negative groups of the RA patients in clinical characteristics, disease activity, severity and inflammatory index analyzed. RESULTS: The positive rate of serum GPI in the patients with RA was 55.4%, contrasting to other autoimmune diseases (14.3%) and healthy controls (7.78%)(P<0.001). Compared with the OA and SpA patients, the RA group was increased more significantly, and the difference was statistically significant (P<0.001). The diagnostic value of GPI alone for RA was 0.39 mg/L, the sensitivity was 54.2%, and specificity was 87.3%. The positive rate of GPI in RF negative patients was 36.1%; the positive rate of GPI in anti-CCP antibody negative patients was 34.2%; the positive rate of GPI in RF and anti-CCP antibody negative patients was 24.1%. The level of GPI had positive correlation (P<0.05) with ESR, RF, anti-CCP antibody and HRF-IgG. CONCLUSION: GPI is sensitive in the patients with RA; GPI positive is important in the diagnosis of RA with anti-CCP antibody and/or RF negative patients. The titer of GPI is related with disease activity of RA.
[Mh] Termos MeSH primário: Artrite Reumatoide/diagnóstico
Autoanticorpos/imunologia
Diagnóstico Precoce
Glucose-6-Fosfato Isomerase/imunologia
[Mh] Termos MeSH secundário: Artrite/classificação
Artrite/diagnóstico
Artrite Reumatoide/imunologia
Autoanticorpos/sangue
Biomarcadores/sangue
Citocinas/sangue
Citocinas/imunologia
Ensaio de Imunoadsorção Enzimática
Feminino
Glucose-6-Fosfato Isomerase/sangue
Seres Humanos
Lúpus Eritematoso Sistêmico
Masculino
Fator Reumatoide/sangue
Fator Reumatoide/imunologia
Escleroderma Sistêmico
Sensibilidade e Especificidade
Síndrome de Sjogren
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Autoantibodies); 0 (Biomarkers); 0 (Cytokines); 9009-79-4 (Rheumatoid Factor); EC 5.3.1.9 (GPI protein, human); EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161218
[St] Status:MEDLINE


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[PMID]:27987493
[Au] Autor:Wu D; Sun L; Li CH; Yang L; Zhao JX; Liu XY
[Ad] Endereço:Department of Rheumatology & Immunology, Peking University Third Hospital, Beijing 100191, China.
[Ti] Título:[Significance of antibodies to the citrullinated glucose-6-phosphate isomerase peptides in rheumatoid arthritis].
[So] Source:Beijing Da Xue Xue Bao Yi Xue Ban;48(6):937-941, 2016 12 18.
[Is] ISSN:1671-167X
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To detect the anti-citrullinated glucose-6-phosphate isomerase (GPI) 70-88 peptide antibody (anti-C-GPI(70-88) antibody), anti-citrullinated GPI 435-453 peptide antibody (anti-C-GPI(435-453) antibody), anti-GPI 70-88 peptide antibody (anti-GPI(70-88) antibody) and anti-GPI 435-453 peptide antibody(anti-GPI(435-453) antibody) in the serum of rheumatoid arthritis (RA) patients, and examine the diagnostic values of the anti-C-GPI peptide antibodies in RA. METHODS: The anti-C-GPI(70-88) antibody, anti-C-GPI(435-453) antibody, anti-GPI(70-88) antibody and anti-GPI(435-453) antibody were detected by enzyme-linked immunosorbent assay (ELISA) in 191 RA patients, 129 other rheumatic diseases and 74 healthy controls. The clinical and laboratory data of the patients with RA were collected, and the values of anti-C-GPI peptide antibodies in the diagnosis of RA and the relationships of anti-C-GPI peptide antibodies with the clinical and laboratory parameters analyzed. RESULTS: (1) The mean titers of the anti-C-GPI(70-88) antibody and the anti-C-GPI(435-453) antibody in the RA patients (respectively, 68.71 ± 4.20 and 51.78 ± 3.13) were significantly higher than those with other rheumatic diseases and healthy individuals (P <0.05). However, the mean titers of the anti-GPI(70-88) antibody and anti-GPI(435-453) antibody in the RA patients were similar to those with other rheumatic diseases and healthy individuals. (2) The diagnostic sensitivity and specificity of the anti-C-GPI(70-88) antibody for RA were 41.88% and 84.50% respectively; and the diagnostic sensitivity and specificity of the anti-C-GPI(435-453) antibody for RA were 46.05% and 86.05% respectively. The sensitivity of combined detection of the two anti-C-GPI peptide antibodies was 50.79%, and the specificity was 81.40%. (3) The positive rates of the anti-C-GPI(70-88) antibody and the anti-C-GPI(435-453) antibody were 35% and 45% respectively in those patients with negative anti-cyclic citrullinated peptide antibody, anti-keratin antibody, and rheumatoid factor. (4) There was no significant difference in clinical and laboratory indicators between the anti-C-GPI(70-88) antibody or anti-C-GPI(435-453) antibody positive group and negative group. CONCLUSION: The anti-C-GPI(70-88) antibody and anti-C-GPI(435-453) antibody can be detected in the serum of RA patients, and C-GPI may be involved in the pathogenesis of RA. There is a certain diagnostic significance for the sera-negative RA.
[Mh] Termos MeSH primário: Artrite Reumatoide/diagnóstico
Autoanticorpos/imunologia
Glucose-6-Fosfato Isomerase/imunologia
[Mh] Termos MeSH secundário: Artrite Reumatoide/imunologia
Autoanticorpos/sangue
Biomarcadores/sangue
Citocinas/sangue
Citocinas/imunologia
Ensaio de Imunoadsorção Enzimática
Feminino
Glucose-6-Fosfato Isomerase/sangue
Seres Humanos
Masculino
Peptídeos Cíclicos
Fator Reumatoide
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Autoantibodies); 0 (Biomarkers); 0 (Cytokines); 0 (Peptides, Cyclic); 0 (cyclic citrullinated peptide); 9009-79-4 (Rheumatoid Factor); EC 5.3.1.9 (GPI protein, human); EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161218
[St] Status:MEDLINE


  10 / 2678 MEDLINE  
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[PMID]:27663407
[Au] Autor:Sánchez-López ÁM; Bahaji A; De Diego N; Baslam M; Li J; Muñoz FJ; Almagro G; García-Gómez P; Ameztoy K; Ricarte-Bermejo A; Novák O; Humplík JF; Spíchal L; Dolezal K; Ciordia S; Mena MC; Navajas R; Baroja-Fernández E; Pozueta-Romero J
[Ad] Endereço:Instituto de Agrobiotecnología, Consejo Superior de Investigaciones Científicas/UPNA/Gobierno de Navarra, 31192 Mutiloabeti, Nafarroa, Spain (A.M.S.-L., A.B., M.B., J.L., F.J.M., G.A., P.G.-G., K.A., A.R.-B., E.B.-F., J.P.-R.).
[Ti] Título:Arabidopsis Responds to Alternaria alternata Volatiles by Triggering Plastid Phosphoglucose Isomerase-Independent Mechanisms.
[So] Source:Plant Physiol;172(3):1989-2001, 2016 Nov.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Volatile compounds (VCs) emitted by phylogenetically diverse microorganisms (including plant pathogens and microbes that do not normally interact mutualistically with plants) promote photosynthesis, growth, and the accumulation of high levels of starch in leaves through cytokinin (CK)-regulated processes. In Arabidopsis (Arabidopsis thaliana) plants not exposed to VCs, plastidic phosphoglucose isomerase (pPGI) acts as an important determinant of photosynthesis and growth, likely as a consequence of its involvement in the synthesis of plastidic CKs in roots. Moreover, this enzyme plays an important role in connecting the Calvin-Benson cycle with the starch biosynthetic pathway in leaves. To elucidate the mechanisms involved in the responses of plants to microbial VCs and to investigate the extent of pPGI involvement, we characterized pPGI-null pgi1-2 Arabidopsis plants cultured in the presence or absence of VCs emitted by Alternaria alternata We found that volatile emissions from this fungal phytopathogen promote growth, photosynthesis, and the accumulation of plastidic CKs in pgi1-2 leaves. Notably, the mesophyll cells of pgi1-2 leaves accumulated exceptionally high levels of starch following VC exposure. Proteomic analyses revealed that VCs promote global changes in the expression of proteins involved in photosynthesis, starch metabolism, and growth that can account for the observed responses in pgi1-2 plants. The overall data show that Arabidopsis plants can respond to VCs emitted by phytopathogenic microorganisms by triggering pPGI-independent mechanisms.
[Mh] Termos MeSH primário: Alternaria/química
Proteínas de Arabidopsis/metabolismo
Arabidopsis/enzimologia
Arabidopsis/microbiologia
Glucose-6-Fosfato Isomerase/metabolismo
Plastídeos/enzimologia
Compostos Orgânicos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Alternaria/efeitos da radiação
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/fisiologia
Parede Celular/metabolismo
Parede Celular/efeitos da radiação
Citocininas/metabolismo
Luz
Células do Mesofilo/efeitos dos fármacos
Células do Mesofilo/metabolismo
Células do Mesofilo/efeitos da radiação
Mutação/genética
Fotossíntese/efeitos da radiação
Plastídeos/efeitos dos fármacos
Proteoma/metabolismo
Amido/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Cytokinins); 0 (Proteome); 0 (Volatile Organic Compounds); 9005-25-8 (Starch); EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE



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