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| [PMID]: | 27806695 |
| [Au] Autor: | van der Burg CA; Prentis PJ; Surm JM; Pavasovic A |
| [Ad] Endereço: | School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, GPO Box 2434, Brisbane, Qld, 4000, Australia. chloe.vanderburg@hdr.qut.edu.au. |
| [Ti] Título: | Insights into the innate immunome of actiniarians using a comparative genomic approach. |
| [So] Source: | BMC Genomics;17(1):850, 2016 11 02. | | [Is] ISSN: | 1471-2164 |
| [Cp] País de publicação: | England |
| [La] Idioma: | eng |
| [Ab] Resumo: | BACKGROUND: Innate immune genes tend to be highly conserved in metazoans, even in early divergent lineages such as Cnidaria (jellyfish, corals, hydroids and sea anemones) and Porifera (sponges). However, constant and diverse selection pressures on the immune system have driven the expansion and diversification of different immune gene families in a lineage-specific manner. To investigate how the innate immune system has evolved in a subset of sea anemone species (Order: Actiniaria), we performed a comprehensive and comparative study using 10 newly sequenced transcriptomes, as well as three publically available transcriptomes, to identify the origins, expansions and contractions of candidate and novel immune gene families. RESULTS: We characterised five conserved genes and gene families, as well as multiple novel innate immune genes, including the newly recognised putative pattern recognition receptor CniFL. Single copies of TLR, MyD88 and NF-κB were found in most species, and several copies of IL-1R-like, NLR and CniFL were found in almost all species. Multiple novel immune genes were identified with domain architectures including the Toll/interleukin-1 receptor (TIR) homology domain, which is well documented as functioning in protein-protein interactions and signal transduction in immune pathways. We hypothesise that these genes may interact as novel proteins in immune pathways of cnidarian species. Novelty in the actiniarian immunome is not restricted to only TIR-domain-containing proteins, as we identify a subset of NLRs which have undergone neofunctionalisation and contain 3-5 N-terminal transmembrane domains, which have so far only been identified in two anthozoan species. CONCLUSIONS: This research has significance in understanding the evolution and origin of the core eumetazoan gene set, including how novel innate immune genes evolve. For example, the evolution of transmembrane domain containing NLRs indicates that these NLRs may be membrane-bound, while all other metazoan and plant NLRs are exclusively cytosolic receptors. This is one example of how species without an adaptive immune system may evolve innovative solutions to detect pathogens or interact with native microbiota. Overall, these results provide an insight into the evolution of the innate immune system, and show that early divergent lineages, such as actiniarians, have a diverse repertoire of conserved and novel innate immune genes. |
| [Mh] Termos MeSH primário: |
Genoma
Genômica
Imunidade Inata/genética
Anêmonas-do-Mar/genética
Anêmonas-do-Mar/imunologia
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| [Mh] Termos MeSH secundário: |
Animais
Biologia Computacional/métodos
Epistasia Genética
Evolução Molecular
Perfilação da Expressão Gênica/métodos
Ontologia Genética
Genômica/métodos
Família Multigênica
Proteína Inibidora de Apoptose Neuronal/genética
Proteína Inibidora de Apoptose Neuronal/metabolismo
Filogenia
Reprodutibilidade dos Testes
Anêmonas-do-Mar/classificação
Transcriptoma
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| [Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (Neuronal Apoptosis-Inhibitory Protein) |
| [Em] Mês de entrada: | 1709 |
| [Cu] Atualização por classe: | 171119 |
[Lr] Data última revisão:
| 171119 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 161104 |
| [St] Status: | MEDLINE |
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