Base de dados : MEDLINE
Pesquisa : D08.811.520.224 [Categoria DeCS]
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[PMID]:29447189
[Au] Autor:Saleem AR; Brunetti C; Khalid A; Della Rocca G; Raio A; Emiliani G; De Carlo A; Mahmood T; Centritto M
[Ad] Endereço:Department of Earth and Environmental Sciences, Bahria University Islamabad Campus, Islamabad, Pakistan.
[Ti] Título:Drought response of Mucuna pruriens (L.) DC. inoculated with ACC deaminase and IAA producing rhizobacteria.
[So] Source:PLoS One;13(2):e0191218, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Drought is one of the major constraints limiting agricultural production worldwide and is expected to increase in the future. Limited water availability causes significant effects to plant growth and physiology. Plants have evolved different traits to mitigate the stress imposed by drought. The presence of plant growth-promoting rhizobacteria (PGPR) could play an important role in improving plant performances and productivity under drought. These beneficial microorganisms colonize the rhizosphere of plants and increase drought tolerance by lowering ethylene formation. In the present study, we demonstrate the potential to improve the growth of velvet bean under water deficit conditions of two different strains of PGPR with ACCd (1-Aminocyclopropane-1-Carboxylate deaminase) activity isolated from rainfed farming system. We compared uninoculated and inoculated plants with PGPR to assess: a) photosynthetic performance and biomass; b) ACC content and ethylene emission from leaves and roots; c) leaf isoprene emission. Our results provided evidence that under drought conditions inoculation with PGPR containing the ACCd enzyme could improve plant growth compared to untreated plants. Ethylene emission from roots and leaves of inoculated velvet bean plants was significantly lower than uninoculated plants. Moreover, isoprene emission increased with drought stress progression and was higher in inoculated plants compared to uninoculated counterparts. These findings clearly illustrate that selected PGPR strains isolated from rainfed areas could be highly effective in promoting plant growth under drought conditions by decreasing ACC and ethylene levels in plants.
[Mh] Termos MeSH primário: Mucuna/metabolismo
Reguladores de Crescimento de Planta/metabolismo
Rhizobiaceae/metabolismo
[Mh] Termos MeSH secundário: Biomassa
Carbono-Carbono Liases/metabolismo
Secas
Etilenos/biossíntese
Mucuna/fisiologia
Fotossíntese
Desenvolvimento Vegetal
Reguladores de Crescimento de Planta/fisiologia
Raízes de Plantas/crescimento & desenvolvimento
Rhizobiaceae/genética
Rizosfera
Microbiologia do Solo
Água/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ethylenes); 0 (Plant Growth Regulators); 059QF0KO0R (Water); 91GW059KN7 (ethylene); EC 3.5.99.7 (1-aminocyclopropane-1-carboxylate deaminase); EC 4.1.- (Carbon-Carbon Lyases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191218


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[PMID]:28923725
[Au] Autor:Baoune H; Ould El Hadj-Khelil A; Pucci G; Sineli P; Loucif L; Polti MA
[Ad] Endereço:Laboratoire de protection des écosystème en zones arides et semi-arides, FNSV, Université Kasdi Merbah Ouragla, 30000, Algeria. Electronic address: baounehafida@hotmail.fr.
[Ti] Título:Petroleum degradation by endophytic Streptomyces spp. isolated from plants grown in contaminated soil of southern Algeria.
[So] Source:Ecotoxicol Environ Saf;147:602-609, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Petroleum hydrocarbons are well known by their high toxicity and recalcitrant properties. Their increasing utilization around worldwide led to environmental contamination. Phytoremediation using plant-associated microbe is an interesting approach for petroleum degradation and actinobacteria have a great potential for that. For this purpose, our study aimed to isolate, characterize, and assess the ability of endophytic actinobacteria to degrade crude petroleum, as well as to produce plant growth promoting traits. Seventeen endophytic actinobacteria were isolated from roots of plants grown naturally in sandy contaminated soil. Among them, six isolates were selected on the basis of their tolerance to petroleum on solid minimal medium and characterized by 16S rDNA gene sequencing. All petroleum-tolerant isolates belonged to the Streptomyces genus. Determination by crude oil degradation by gas chromatorgraph-flame ionization detector revealed that five strains could use petroleum as sole carbon and energy source and the petroleum removal achieved up to 98% after 7 days of incubation. These isolates displayed an important role in the degradation of the n-alkanes (C -C ), aromatic and polycyclic aromatic hydrocarbons. All strains showed a wide range of plant growth promoting features such as siderophores, phosphate solubilization, 1-aminocyclopropane-1-carboxylate deaminase, nitrogen fixation and indole-3-acetic acid production as well as biosurfactant production. This is the first study highlighting the petroleum degradation ability and plant growth promoting attributes of endophytic Streptomyces. The finding suggests that the endophytic actinobacteria isolated are promising candidates for improving phytoremediation efficiency of petroleum contaminated soil.
[Mh] Termos MeSH primário: Endófitos/metabolismo
Petróleo/análise
Plantas/microbiologia
Poluentes do Solo/análise
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Argélia
Biodegradação Ambiental
Carbono-Carbono Liases/metabolismo
Endófitos/crescimento & desenvolvimento
Fixação de Nitrogênio/efeitos dos fármacos
Desenvolvimento Vegetal/efeitos dos fármacos
Plantas/metabolismo
Solo/química
Streptomyces/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Petroleum); 0 (Soil); 0 (Soil Pollutants); EC 3.5.99.7 (1-aminocyclopropane-1-carboxylate deaminase); EC 4.1.- (Carbon-Carbon Lyases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


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[PMID]:28460054
[Au] Autor:Zhou N; Zhao S; Tian CY
[Ad] Endereço:State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.
[Ti] Título:Effect of halotolerant rhizobacteria isolated from halophytes on the growth of sugar beet (Beta vulgaris L.) under salt stress.
[So] Source:FEMS Microbiol Lett;364(11), 2017 Jun 15.
[Is] ISSN:1574-6968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Utilization of rhizobacteria that have associated with plant roots in harsh environments could be a feasible strategy to deal with limits to agricultural production caused by soil salinity. Halophytes occur naturally in high-salt environments, and their roots may be associated with promising microbial candidates for promoting growth and salt tolerance in crops. This study aimed to isolate efficient halotolerant plant-growth-promoting rhizobacterial strains from halophytes and evaluate their activity and effects on sugar beet (Beta vulgaris L.) growth under salinity stress. A total of 23 isolates were initially screened for their ability to secrete 1-aminocyclopropane-1-carboxylate deaminase (ACD) as well as other plant-growth-promoting characteristics and subsequently identified by sequencing the 16S rRNA gene. Three isolates, identified as Micrococcus yunnanensis, Planococcus rifietoensis and Variovorax paradoxus, enhanced salt stress tolerance remarkably in sugar beet, resulting in greater seed germination and plant biomass, higher photosynthetic capacity and lower stress-induced ethylene production at different NaCl concentrations (50-125 mM). These results demonstrate that salinity-adapted, ACD-producing bacteria isolated from halophytes could promote sugar beet growth under saline stress conditions.
[Mh] Termos MeSH primário: Alphaproteobacteria/classificação
Beta vulgaris/microbiologia
Raízes de Plantas/microbiologia
Plantas Tolerantes a Sal/microbiologia
Estresse Fisiológico
[Mh] Termos MeSH secundário: Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Beta vulgaris/crescimento & desenvolvimento
Biomassa
Carbono-Carbono Liases/metabolismo
Etilenos/metabolismo
Micrococcus/isolamento & purificação
Micrococcus/metabolismo
Bactérias Planococcus/isolamento & purificação
Bactérias Planococcus/metabolismo
RNA Ribossômico 16S/genética
Salinidade
Solo/química
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenes); 0 (RNA, Ribosomal, 16S); 0 (Soil); 91GW059KN7 (ethylene); EC 3.5.99.7 (1-aminocyclopropane-1-carboxylate deaminase); EC 4.1.- (Carbon-Carbon Lyases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1093/femsle/fnx091


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[PMID]:28456298
[Au] Autor:Maeda K; Nakajima Y; Motoyama T; Kondoh Y; Kawamura T; Kanamaru K; Ohsato S; Nishiuchi T; Yoshida M; Osada H; Kobayashi T; Kimura M
[Ad] Endereço:Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan; Graduate School of Agriculture, Meiji University, 1-1-1 Higashi-Mita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
[Ti] Título:Identification of a trichothecene production inhibitor by chemical array and library screening using trichodiene synthase as a target protein.
[So] Source:Pestic Biochem Physiol;138:1-7, 2017 May.
[Is] ISSN:1095-9939
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Trichothecene mycotoxins often accumulate in apparently normal grains of cereal crops. In an effort to develop an agricultural chemical to reduce trichothecene contamination, we screened trichothecene production inhibitors from the compounds on the chemical arrays. By using the trichodiene (TDN) synthase tagged with hexahistidine (rTRI5) as a target protein, 32 hit compounds were obtained from chemical library of the RIKEN Natural Product Depository (NPDepo) by chemical array screening. At 10µgmL , none of the 32 chemicals inhibited trichothecene production by Fusarium graminearum in liquid culture. Against the purified rTRI5 enzyme, however, NPD10133 [progesterone 3-(O-carboxymethyl)oxime amide-bonded to phenylalanine] showed weak inhibitory activity at 10µgmL (18.7µM). For the screening of chemicals inhibiting trichothecene accumulation in liquid culture, 20 analogs of NPD10133 selected from the NPDepo chemical library were assayed. At 10µM, only NPD352 [testosterone 3-(O-carboxymethyl)oxime amide-bonded to phenylalanine methyl ester] inhibited rTRI5 activity and trichothecene production. Kinetic analysis suggested that the enzyme inhibition was of a mixed-type. The identification of NPD352 as a TDN synthase inhibitor lays the foundation for the development of a more potent inhibitor via systematic introduction of wide structural diversity on the gonane skeleton and amino acid residues.
[Mh] Termos MeSH primário: Carbono-Carbono Liases/antagonistas & inibidores
Fungicidas Industriais/química
Fungicidas Industriais/farmacologia
Fusarium/metabolismo
Tricotecenos/antagonistas & inibidores
[Mh] Termos MeSH secundário: Inibidores Enzimáticos
Bibliotecas de Moléculas Pequenas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Fungicides, Industrial); 0 (Small Molecule Libraries); 0 (Trichothecenes); EC 4.1.- (Carbon-Carbon Lyases); EC 4.2.3.6 (trichodiene synthetase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


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[PMID]:28967743
[Au] Autor:Blank PN; Barrow GH; Chou WKW; Duan L; Cane DE; Christianson DW
[Ad] Endereço:Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania , Philadelphia, Pennsylvania 19104-6323, United States.
[Ti] Título:Substitution of Aromatic Residues with Polar Residues in the Active Site Pocket of epi-Isozizaene Synthase Leads to the Generation of New Cyclic Sesquiterpenes.
[So] Source:Biochemistry;56(43):5798-5811, 2017 Oct 31.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The sesquiterpene cyclase epi-isozizaene synthase (EIZS) catalyzes the cyclization of farnesyl diphosphate to form the tricyclic hydrocarbon precursor of the antibiotic albaflavenone. The hydrophobic active site pocket of EIZS serves as a template as it binds and chaperones the flexible substrate and carbocation intermediates through the conformations required for a multistep reaction sequence. We previously demonstrated that the substitution of hydrophobic residues with other hydrophobic residues remolds the template and expands product chemodiversity [Li, R., Chou, W. K. W., Himmelberger, J. A., Litwin, K. M., Harris, G. G., Cane, D. E., and Christianson, D. W. (2014) Biochemistry 53, 1155-1168]. Here, we show that the substitution of hydrophobic residues-specifically, Y69, F95, F96, and W203-with polar side chains also yields functional enzyme catalysts that expand product chemodiversity. Fourteen new EIZS mutants are reported that generate product arrays in which eight new sesquiterpene products have been identified. Of note, some mutants generate acyclic and cyclic hydroxylated products, suggesting that the introduction of polarity in the hydrophobic pocket facilitates the binding of water capable of quenching carbocation intermediates. Furthermore, the substitution of polar residues for F96 yields high-fidelity sesquisabinene synthases. Crystal structures of selected mutants reveal that residues defining the three-dimensional contour of the hydrophobic pocket can be substituted without triggering significant structural changes elsewhere in the active site. Thus, more radical nonpolar-polar amino acid substitutions should be considered when terpenoid cyclase active sites are remolded by mutagenesis with the goal of exploring and expanding product chemodiversity.
[Mh] Termos MeSH primário: Substituição de Aminoácidos
Proteínas de Bactérias/química
Carbono-Carbono Liases/química
Modelos Moleculares
Streptomyces coelicolor/enzimologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Carbono-Carbono Liases/genética
Carbono-Carbono Liases/metabolismo
Domínio Catalítico
Cristalografia por Raios X
Interações Hidrofóbicas e Hidrofílicas
Mutação de Sentido Incorreto
Sesquiterpenos/química
Sesquiterpenos/metabolismo
Streptomyces coelicolor/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Sesquiterpenes); EC 4.1.- (Carbon-Carbon Lyases); EC 4.2.3.6 (trichodiene synthetase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00895


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[PMID]:28841019
[Au] Autor:Christianson DW
[Ad] Endereço:Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania , 231 South 34th Street, Philadelphia, Pennsylvania 19104-6323, United States.
[Ti] Título:Structural and Chemical Biology of Terpenoid Cyclases.
[So] Source:Chem Rev;117(17):11570-11648, 2017 Sep 13.
[Is] ISSN:1520-6890
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The year 2017 marks the twentieth anniversary of terpenoid cyclase structural biology: a trio of terpenoid cyclase structures reported together in 1997 were the first to set the foundation for understanding the enzymes largely responsible for the exquisite chemodiversity of more than 80000 terpenoid natural products. Terpenoid cyclases catalyze the most complex chemical reactions in biology, in that more than half of the substrate carbon atoms undergo changes in bonding and hybridization during a single enzyme-catalyzed cyclization reaction. The past two decades have witnessed structural, functional, and computational studies illuminating the modes of substrate activation that initiate the cyclization cascade, the management and manipulation of high-energy carbocation intermediates that propagate the cyclization cascade, and the chemical strategies that terminate the cyclization cascade. The role of the terpenoid cyclase as a template for catalysis is paramount to its function, and protein engineering can be used to reprogram the cyclization cascade to generate alternative and commercially important products. Here, I review key advances in terpenoid cyclase structural and chemical biology, focusing mainly on terpenoid cyclases and related prenyltransferases for which X-ray crystal structures have informed and advanced our understanding of enzyme structure and function.
[Mh] Termos MeSH primário: Alquil e Aril Transferases/química
Terpenos/metabolismo
[Mh] Termos MeSH secundário: Alquil e Aril Transferases/metabolismo
Carbono-Carbono Liases/química
Carbono-Carbono Liases/metabolismo
Cristalografia por Raios X
Dimetilaliltranstransferase/química
Dimetilaliltranstransferase/metabolismo
Liases Intramoleculares/química
Liases Intramoleculares/metabolismo
Estrutura Terciária de Proteína
Terpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Terpenes); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.- (terpene carbocyclase); EC 2.5.1.1 (Dimethylallyltranstransferase); EC 4.1.- (Carbon-Carbon Lyases); EC 4.2.3.6 (trichodiene synthetase); EC 5.5.- (Intramolecular Lyases); EC 5.5.- (pinene cyclase I)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.1021/acs.chemrev.7b00287


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[PMID]:28771547
[Au] Autor:Dutta J; Thakur D
[Ad] Endereço:Microbial Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, India.
[Ti] Título:Evaluation of multifarious plant growth promoting traits, antagonistic potential and phylogenetic affiliation of rhizobacteria associated with commercial tea plants grown in Darjeeling, India.
[So] Source:PLoS One;12(8):e0182302, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant growth promoting rhizobacteria (PGPR) are studied in different agricultural crops but the interaction of PGPR of tea crop is not yet studied well. In the present study, the indigenous tea rhizobacteria were isolated from seven tea estates of Darjeeling located in West Bengal, India. A total of 150 rhizobacterial isolates were screened for antagonistic activity against six different fungal pathogens i.e. Nigrospora sphaerica (KJ767520), Pestalotiopsis theae (ITCC 6599), Curvularia eragostidis (ITCC 6429), Glomerella cingulata (MTCC 2033), Rhizoctonia Solani (MTCC 4633) and Fusarium oxysporum (MTCC 284), out of which 48 isolates were antagonist to at least one fungal pathogen used. These 48 isolates exhibited multifarious antifungal properties like the production of siderophore, chitinase, protease and cellulase and also plant growth promoting (PGP) traits like IAA production, phosphate solubilization, ammonia and ACC deaminase production. Amplified ribosomal DNA restriction analysis (ARDRA) and BOX-PCR analysis based genotyping clustered the isolates into different groups. Finally, four isolates were selected for plant growth promotion study in two tea commercial cultivars TV-1 and Teenali-17 in nursery conditions. The plant growth promotion study showed that the inoculation of consortia of these four PGPR isolates significantly increased the growth of tea plant in nursery conditions. Thus this study underlines the commercial potential of these selected PGPR isolates for sustainable tea cultivation.
[Mh] Termos MeSH primário: Alphaproteobacteria/classificação
Alphaproteobacteria/metabolismo
Camellia sinensis/crescimento & desenvolvimento
Camellia sinensis/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Alphaproteobacteria/isolamento & purificação
Amônia/metabolismo
Antifúngicos/metabolismo
Antifúngicos/farmacologia
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Fosfatos de Cálcio/metabolismo
Carbono-Carbono Liases/metabolismo
Celulase/genética
Celulase/metabolismo
Quitinases/genética
Quitinases/metabolismo
DNA Fúngico/isolamento & purificação
DNA Fúngico/metabolismo
Fungos/efeitos dos fármacos
Genótipo
Glicosídeo Hidrolases/genética
Glicosídeo Hidrolases/metabolismo
Índia
Ácidos Indolacéticos/metabolismo
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/microbiologia
RNA Ribossômico 16S/classificação
RNA Ribossômico 16S/isolamento & purificação
RNA Ribossômico 16S/metabolismo
Sideróforos/metabolismo
Sideróforos/farmacologia
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Bacterial Proteins); 0 (Calcium Phosphates); 0 (DNA, Fungal); 0 (Indoleacetic Acids); 0 (RNA, Ribosomal, 16S); 0 (Siderophores); 6U1S09C61L (indoleacetic acid); 7664-41-7 (Ammonia); EC 3.2.1.- (Glycoside Hydrolases); EC 3.2.1.14 (Chitinases); EC 3.2.1.4 (Cellulase); EC 3.5.99.7 (1-aminocyclopropane-1-carboxylate deaminase); EC 4.1.- (Carbon-Carbon Lyases); K4C08XP666 (tricalcium phosphate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182302


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[PMID]:28686889
[Au] Autor:Ma Y; Rajkumar M; Moreno A; Zhang C; Freitas H
[Ad] Endereço:Centre for Functional Ecology, Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal. Electronic address: cathymaying@gmail.com.
[Ti] Título:Serpentine endophytic bacterium Pseudomonas azotoformans ASS1 accelerates phytoremediation of soil metals under drought stress.
[So] Source:Chemosphere;185:75-85, 2017 Oct.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study evaluates the potential of serpentine endophytic bacterium to foster phytoremediation efficiency of Trifolium arvense grown on multi-metal (Cu, Zn and Ni) contaminated soils under drought stress. A drought resistant endophytic bacterial strain ASS1 isolated from the leaves of Alyssum serpyllifolium grown in serpentine soils was identified as Pseudomonas azotoformans based on biochemical tests and partial 16S rRNA gene sequencing. P. azotoformans ASS1 possessed abiotic stress resistance (heavy metals, drought, salinity, antibiotics and extreme temperature) and plant growth promoting (PGP) properties (phosphate solubilization, nitrogen fixation, production of 1-aminocyclopropane-1-carboxylate deaminase, siderophore and ammonia). Inoculation of T. arvense with ASS1 considerably increased the plant biomass and leaf relative water content in both roll towel assay and pot experiments in the absence and presence of drought stress (DS). In the pot experiments, ASS1 greatly enhanced chlorophyll content, catalase, peroxidase, superoxide dismutase activities, and proline content (only in the absence of drought) in plant leaves, whereas they decreased the concentrations of malondialdehyde. Irrespective of water stress, ASS1 significantly improved accumulation, total removal, bio-concentration factor and biological accumulation coefficient of metals (Cu, Zn and Ni), while decreased translocation factors of Cu. The effective colonization and survival in the rhizosphere and tissue interior assured improved plant growth and successful metal phytoremediation under DS. These results demonstrate the potential of serpentine endophytic bacterium ASS1 for protecting plants against abiotic stresses and helping plants to thrive in semiarid ecosystems and accelerate phytoremediation process in metal polluted soils.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Secas
Metais Pesados/metabolismo
Pseudomonas/fisiologia
Microbiologia do Solo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Asbestos Serpentinas
Bactérias/genética
Biomassa
Carbono-Carbono Liases
Metais Pesados/análise
Pseudomonas/genética
RNA Ribossômico 16S/genética
Rizosfera
Sideróforos
Solo
Poluentes do Solo/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Asbestos, Serpentine); 0 (Metals, Heavy); 0 (RNA, Ribosomal, 16S); 0 (Siderophores); 0 (Soil); 0 (Soil Pollutants); EC 3.5.99.7 (1-aminocyclopropane-1-carboxylate deaminase); EC 4.1.- (Carbon-Carbon Lyases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE


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[PMID]:28369150
[Au] Autor:Meyer-Cifuentes I; Martinez-Lavanchy PM; Marin-Cevada V; Böhnke S; Harms H; Müller JA; Heipieper HJ
[Ad] Endereço:Helmholtz Centre for Environmental Research-UFZ, Department of Environmental Biotechnology, Leipzig, Germany.
[Ti] Título:Isolation and characterization of Magnetospirillum sp. strain 15-1 as a representative anaerobic toluene-degrader from a constructed wetland model.
[So] Source:PLoS One;12(4):e0174750, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previously, Planted Fixed-Bed Reactors (PFRs) have been used to investigate microbial toluene removal in the rhizosphere of constructed wetlands. Aerobic toluene degradation was predominant in these model systems although bulk redox conditions were hypoxic to anoxic. However, culture-independent approaches indicated also that microbes capable of anaerobic toluene degradation were abundant. Therefore, we aimed at isolating anaerobic-toluene degraders from one of these PFRs. From the obtained colonies which consisted of spirilli-shaped bacteria, a strain designated 15-1 was selected for further investigations. Analysis of its 16S rRNA gene revealed greatest similarity (99%) with toluene-degrading Magnetospirillum sp. TS-6. Isolate 15-1 grew with up to 0.5 mM of toluene under nitrate-reducing conditions. Cells reacted to higher concentrations of toluene by an increase in the degree of saturation of their membrane fatty acids. Strain 15-1 contained key genes for the anaerobic degradation of toluene via benzylsuccinate and subsequently the benzoyl-CoA pathway, namely bssA, encoding for the alpha subunit of benzylsuccinate synthase, bcrC for subunit C of benzoyl-CoA reductase and bamA for 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase. Finally, most members of a clone library of bssA generated from the PFR had highest similarity to bssA from strain 15-1. Our study provides insights about the physiological capacities of a strain of Magnetospirillum isolated from a planted system where active rhizoremediation of toluene is taking place.
[Mh] Termos MeSH primário: Magnetospirillum/isolamento & purificação
Magnetospirillum/metabolismo
Rizosfera
Tolueno/metabolismo
Zonas Úmidas
[Mh] Termos MeSH secundário: Carbono/metabolismo
Carbono-Carbono Liases/genética
Ácidos Graxos/metabolismo
Magnetospirillum/genética
Lipídeos de Membrana/metabolismo
Nitratos/metabolismo
Oxigênio/metabolismo
Filogenia
Raízes de Plantas/microbiologia
RNA Ribossômico 16S/genética
Homologia de Sequência de Aminoácidos
Microbiologia do Solo
Tolueno/toxicidade
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Membrane Lipids); 0 (Nitrates); 0 (RNA, Ribosomal, 16S); 3FPU23BG52 (Toluene); 7440-44-0 (Carbon); EC 4.1.- (Carbon-Carbon Lyases); EC 4.1.99.- (benzylsuccinate synthase); S88TT14065 (Oxygen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174750


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[PMID]:28288107
[Au] Autor:Li S; Lowell AN; Newmister SA; Yu F; Williams RM; Sherman DH
[Ad] Endereço:Life Sciences Institute, University of Michigan, Ann Arbor, Michigan, USA.
[Ti] Título:Decoding cyclase-dependent assembly of hapalindole and fischerindole alkaloids.
[So] Source:Nat Chem Biol;13(5):467-469, 2017 May.
[Is] ISSN:1552-4469
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The formation of C-C bonds in an enantioselective fashion to create complex polycyclic scaffolds in the hapalindole- and fischerindole- type alkaloids from Stigonematales cyanobacteria represents a compelling and urgent challenge in adapting microbial biosynthesis as a catalytic platform in drug development. Here we determine the biochemical basis for tri- and tetracyclic core formation in these secondary metabolites, involving a new class of cyclases that catalyze a complex cyclization cascade.
[Mh] Termos MeSH primário: Biocatálise
Carbono-Carbono Liases/metabolismo
Alcaloides de Indol/metabolismo
Indóis/metabolismo
[Mh] Termos MeSH secundário: Cianobactérias/metabolismo
Ciclização
Alcaloides de Indol/química
Indóis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indole Alkaloids); 0 (Indoles); 0 (fischerindole); EC 4.1.- (Carbon-Carbon Lyases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.1038/nchembio.2327



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