Base de dados : MEDLINE
Pesquisa : D08.811.520.224.125.100 [Categoria DeCS]
Referências encontradas : 914 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 92 ir para página                         

  1 / 914 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28459438
[Au] Autor:Li Y; Lucas-Osma AM; Black S; Bandet MV; Stephens MJ; Vavrek R; Sanelli L; Fenrich KK; Di Narzo AF; Dracheva S; Winship IR; Fouad K; Bennett DJ
[Ad] Endereço:Neuroscience and Mental Health Institute and Faculty of Rehabilitation Medicine, University of Alberta, Edmonton, Alberta, Canada.
[Ti] Título:Pericytes impair capillary blood flow and motor function after chronic spinal cord injury.
[So] Source:Nat Med;23(6):733-741, 2017 Jun.
[Is] ISSN:1546-170X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Blood vessels in the central nervous system (CNS) are controlled by neuronal activity. For example, widespread vessel constriction (vessel tone) is induced by brainstem neurons that release the monoamines serotonin and noradrenaline, and local vessel dilation is induced by glutamatergic neuron activity. Here we examined how vessel tone adapts to the loss of neuron-derived monoamines after spinal cord injury (SCI) in rats. We find that, months after the imposition of SCI, the spinal cord below the site of injury is in a chronic state of hypoxia owing to paradoxical excess activity of monoamine receptors (5-HT ) on pericytes, despite the absence of monoamines. This monoamine-receptor activity causes pericytes to locally constrict capillaries, which reduces blood flow to ischemic levels. Receptor activation in the absence of monoamines results from the production of trace amines (such as tryptamine) by pericytes that ectopically express the enzyme aromatic L-amino acid decarboxylase (AADC), which synthesizes trace amines directly from dietary amino acids (such as tryptophan). Inhibition of monoamine receptors or of AADC, or even an increase in inhaled oxygen, produces substantial relief from hypoxia and improves motoneuron and locomotor function after SCI.
[Mh] Termos MeSH primário: Monoaminas Biogênicas/metabolismo
Capilares/metabolismo
Hipóxia/metabolismo
Locomoção/fisiologia
Pericitos/metabolismo
Traumatismos da Medula Espinal/metabolismo
Vasoconstrição
[Mh] Termos MeSH secundário: Animais
Descarboxilases de Aminoácido-L-Aromático/metabolismo
Capilares/efeitos dos fármacos
Capilares/patologia
Capilares/fisiopatologia
Injeções Espinhais
Locomoção/efeitos dos fármacos
Microscopia Confocal
Microscopia de Interferência
Norepinefrina/metabolismo
Oxigênio/metabolismo
Oxigenoterapia
RNA Mensageiro/metabolismo
Ratos
Receptor 5-HT1B de Serotonina/metabolismo
Receptores Adrenérgicos alfa 2/metabolismo
Receptores 5-HT1 de Serotonina/metabolismo
Serotonina/metabolismo
Antagonistas do Receptor 5-HT1 de Serotonina/farmacologia
Traumatismos da Medula Espinal/patologia
Traumatismos da Medula Espinal/fisiopatologia
Transcriptoma
Triptaminas/metabolismo
Tiramina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biogenic Monoamines); 0 (RNA, Messenger); 0 (Receptor, Serotonin, 5-HT1B); 0 (Receptors, Adrenergic, alpha-2); 0 (Receptors, Serotonin, 5-HT1); 0 (Serotonin 5-HT1 Receptor Antagonists); 0 (Tryptamines); 333DO1RDJY (Serotonin); 422ZU9N5TV (tryptamine); EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases); S88TT14065 (Oxygen); X4W3ENH1CV (Norepinephrine); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171206
[Lr] Data última revisão:
171206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1038/nm.4331


  2 / 914 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28622392
[Au] Autor:Wang L; Wang Z; Zhu R; Bi J; Feng X; Liu W; Wu J; Zhang H; Wu H; Kong W; Yu B; Yu X
[Ad] Endereço:National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin Province, China.
[Ti] Título:Therapeutic efficacy of AAV8-mediated intrastriatal delivery of human cerebral dopamine neurotrophic factor in 6-OHDA-induced parkinsonian rat models with different disease progression.
[So] Source:PLoS One;12(6):e0179476, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Parkinson's disease (PD) is a progressive and age-associated neurodegenerative disorder. Patients at different stages of the disease course have distinguished features, mainly in the number of dopaminergic neurons. Cerebral dopamine neurotrophic factor (CDNF) is a recently discovered neurotrophic factor, being deemed as a hopeful candidate for PD treatment. Here, we evaluated the efficacy of CDNF in protecting dopaminergic function using the 6-OHDA-induced PD rat model suffering from different levels of neuronal loss and the recombinant adeno-associated virus 8 (AAV8) as a carrier for the CDNF gene. The results showed that AAV8-CDNF administration significantly improved the motor function and increased the tyrosine hydroxylase (TH) levels in PD rats with mild lesions (2 weeks post lesion), but it had limited therapeutic effects in rats with severe lesions (5 weeks post lesion). To better improve the recovery of motor function in severely lesioned PD rats, we employed a strategy using the CDNF gene along with the aromatic amino acid decarboxylase (AADC) gene. This combination therapeutic strategy indeed showed an enhanced benefit in restoring the motor function of severely lesioned PD rats by providing the neuroprotective effect of CDNF and dopamine enhancing effect of AADC as expected. This study may provide a basis for future clinical application of CDNF in PD patients at different stages and offer a new alternative strategy of joint use of CDNF and AADC for advanced PD patients in clinical trials.
[Mh] Termos MeSH primário: Corpo Estriado
Dependovirus
Terapia Genética/métodos
Atividade Motora
Fatores de Crescimento Neural
Oxidopamina/efeitos adversos
Doença de Parkinson Secundária
Recuperação de Função Fisiológica
Transdução Genética/métodos
[Mh] Termos MeSH secundário: Animais
Descarboxilases de Aminoácido-L-Aromático/biossíntese
Descarboxilases de Aminoácido-L-Aromático/genética
Corpo Estriado/metabolismo
Corpo Estriado/fisiopatologia
Seres Humanos
Masculino
Fatores de Crescimento Neural/biossíntese
Fatores de Crescimento Neural/genética
Oxidopamina/farmacologia
Doença de Parkinson Secundária/induzido quimicamente
Doença de Parkinson Secundária/genética
Doença de Parkinson Secundária/metabolismo
Doença de Parkinson Secundária/terapia
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CDNF protein, human); 0 (Nerve Growth Factors); 8HW4YBZ748 (Oxidopamine); EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases); EC 4.1.1.28 (DDC protein, human)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179476


  3 / 914 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28552562
[Au] Autor:Yuan J; Gilbert ER; Cline MA
[Ad] Endereço:National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People's Republic of China; Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, United States.
[Ti] Título:The central anorexigenic mechanism of amylin in Japanese quail (Coturnix japonica) involves pro-opiomelanocortin, calcitonin receptor, and the arcuate nucleus of the hypothalamus.
[So] Source:Comp Biochem Physiol A Mol Integr Physiol;210:28-34, 2017 Aug.
[Is] ISSN:1531-4332
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Amylin is a 37-amino acid peptide hormone that exerts anorexigenic effects in humans and animals. We demonstrated that central injection of amylin into chicks affected feeding and related behaviors via the hypothalamus and brainstem, although the molecular mechanisms remained elusive. Thus, the objective of this study was to investigate the molecular mechanisms underlying anorexigenic effects of amylin in 7 day-old Japanese quail. Food but not water intake was reduced after intracerebroventricular amylin injection, and the behavior analysis indicated that this was associated with decreased food pecks and preening. Whole hypothalamus and hypothalamic nuclei including the arcuate nucleus (ARC), paraventricular nucleus (PVN), ventromedial hypothalamus (VMH), dorsomedial nucleus (DMN) and lateral hypothalamic area (LH) were extracted from quail at 1h post-injection for total RNA isolation. Real time PCR was performed to quantify mRNA abundance of amylin receptors, appetite-associated neuropeptides and monoamine-synthesis-related enzymes. Central amylin injection increased the mRNA abundance of calcitonin receptor (CALCR), receptor activity modifying protein 1 (RAMP1), pro-opiomelanocortin (POMC), and aromatic l-amino acid decarboxylase (AADC) in the hypothalamus and individual hypothalamic nuclei. Relative quantities of CALCR and POMC mRNA were greater in the ARC of the amylin- than vehicle-treated group. Thus, amylin-mediated effects on food intake may involve POMC, monoamine synthesis, and amylin receptor 1 (a complex of CALCR and RAMP1) in the ARC. Together, these data provide novel insights on the hypothalamic-specific molecular mechanisms of amylin-induced food intake.
[Mh] Termos MeSH primário: Coturnix/fisiologia
Ingestão de Alimentos
Polipeptídeo Amiloide das Ilhotas Pancreáticas/metabolismo
Pró-Opiomelanocortina/metabolismo
Receptores da Calcitonina/metabolismo
[Mh] Termos MeSH secundário: Animais
Apetite/fisiologia
Núcleo Arqueado do Hipotálamo/efeitos dos fármacos
Núcleo Arqueado do Hipotálamo/metabolismo
Núcleo Arqueado do Hipotálamo/fisiologia
Descarboxilases de Aminoácido-L-Aromático/genética
Descarboxilases de Aminoácido-L-Aromático/metabolismo
Ingestão de Líquidos
Ingestão de Alimentos/efeitos dos fármacos
Regulação da Expressão Gênica/efeitos dos fármacos
Hipotálamo/efeitos dos fármacos
Hipotálamo/fisiologia
Polipeptídeo Amiloide das Ilhotas Pancreáticas/farmacologia
Pró-Opiomelanocortina/genética
Proteína 1 Modificadora da Atividade de Receptores/genética
Proteína 1 Modificadora da Atividade de Receptores/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Islet Amyloid Polypeptide); 0 (Receptor Activity-Modifying Protein 1); 0 (Receptors, Calcitonin); 66796-54-1 (Pro-Opiomelanocortin); EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170530
[St] Status:MEDLINE


  4 / 914 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28336394
[Au] Autor:Shimohata A; Ishihara K; Hattori S; Miyamoto H; Morishita H; Ornthanalai G; Raveau M; Ebrahim AS; Amano K; Yamada K; Sago H; Akiba S; Mataga N; Murphy NP; Miyakawa T; Yamakawa K
[Ad] Endereço:Laboratory for Neurogenetics, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
[Ti] Título:Ts1Cje Down syndrome model mice exhibit environmental stimuli-triggered locomotor hyperactivity and sociability concurrent with increased flux through central dopamine and serotonin metabolism.
[So] Source:Exp Neurol;293:1-12, 2017 Jul.
[Is] ISSN:1090-2430
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ts1Cje mice have a segmental trisomy of chromosome 16 that is orthologous to human chromosome 21 and display Down syndrome-like cognitive impairments. Despite the occurrence of affective and emotional impairments in patients with Down syndrome, these parameters are poorly documented in Down syndrome mouse models, including Ts1Cje mice. Here, we conducted comprehensive behavioral analyses, including anxiety-, sociability-, and depression-related tasks, and biochemical analyses of monoamines and their metabolites in Ts1Cje mice. Ts1Cje mice showed enhanced locomotor activity in novel environments and increased social contact with unfamiliar partners when compared with wild-type littermates, but a significantly lower activity in familiar environments. Ts1Cje mice also exhibited some signs of decreased depression like-behavior. Furthermore, Ts1Cje mice showed monoamine abnormalities, including increased extracellular dopamine and serotonin, and enhanced catabolism in the striatum and ventral forebrain. This study constitutes the first report of deviated monoamine metabolism that may help explain the basis for abnormal behaviors, including the environmental stimuli-triggered hyperactivity, increased sociability and decreased depression-like behavior in Ts1Cje mice.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Transtornos Cognitivos/etiologia
Dopamina/metabolismo
Síndrome de Down
Meio Ambiente
Hipercinese/etiologia
Serotonina/metabolismo
[Mh] Termos MeSH secundário: Aldeído Desidrogenase/metabolismo
Animais
Descarboxilases de Aminoácido-L-Aromático/metabolismo
Catecol O-Metiltransferase/metabolismo
Cromossomos Humanos Par 16/genética
Modelos Animais de Doenças
Síndrome de Down/complicações
Síndrome de Down/genética
Síndrome de Down/patologia
Comportamento Exploratório
Feminino
Hipercinese/genética
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Mutantes
Trissomia/genética
Tirosina 3-Mono-Oxigenase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
333DO1RDJY (Serotonin); EC 1.14.16.2 (Tyrosine 3-Monooxygenase); EC 1.2.1.3 (ALDH1A1 protein, mouse); EC 1.2.1.3 (Aldehyde Dehydrogenase); EC 2.1.1.6 (COMT protein, mouse); EC 2.1.1.6 (Catechol O-Methyltransferase); EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE


  5 / 914 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28294334
[Au] Autor:Becker G; Bahri MA; Michel A; Hustadt F; Garraux G; Luxen A; Lemaire C; Plenevaux A
[Ad] Endereço:GIGA - CRC In vivo Imaging, University of Liège, Liège, Belgium.
[Ti] Título:Comparative assessment of 6-[ F]fluoro-L-m-tyrosine and 6-[ F]fluoro-L-dopa to evaluate dopaminergic presynaptic integrity in a Parkinson's disease rat model.
[So] Source:J Neurochem;141(4):626-635, 2017 May.
[Is] ISSN:1471-4159
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Because of the progressive loss of nigro-striatal dopaminergic terminals in Parkinson's disease (PD), in vivo quantitative imaging of dopamine (DA) containing neurons in animal models of PD is of critical importance in the preclinical evaluation of highly awaited disease-modifying therapies. Among existing methods, the high sensitivity of positron emission tomography (PET) is attractive to achieve that goal. The aim of this study was to perform a quantitative comparison of brain images obtained in 6-hydroxydopamine (6-OHDA) lesioned rats using two dopaminergic PET radiotracers, namely [ F]fluoro-3,4-dihydroxyphenyl-L-alanine ([ F]FDOPA) and 6-[ F]fluoro-L-m-tyrosine ([ F]FMT). Because the imaging signal is theoretically less contaminated by metabolites, we hypothesized that the latter would show stronger relationship with behavioural and post-mortem measures of striatal dopaminergic deficiency. We used a within-subject design to measure striatal [ F]FMT and [ F]FDOPA uptake in eight partially lesioned, eight fully lesioned and ten sham-treated rats. Animals were pretreated with an L-aromatic amino acid decarboxylase inhibitor. A catechol-O-methyl transferase inhibitor was also given before [ F]FDOPA PET. Quantitative estimates of striatal uptake were computed using conventional graphical Patlak method. Striatal dopaminergic deficiencies were measured with apomorphine-induced rotations and post-mortem striatal DA content. We observed a strong relationship between [ F]FMT and [ F]FDOPA estimates of decreased uptake in the denervated striatum using the tissue-derived uptake rate constant K . However, only [ F]FMT K succeeded to discriminate between the partial and the full 6-OHDA lesion and correlated well with the post-mortem striatal DA content. This study indicates that the [ F]FMT could be more sensitive, with respect of [ F]FDOPA, to investigate DA terminals loss in 6-OHDA rats, and open the way to in vivo L-aromatic amino acid decarboxylase activity targeting in future investigations on progressive PD models.
[Mh] Termos MeSH primário: Di-Hidroxifenilalanina/análogos & derivados
Doença de Parkinson Secundária/diagnóstico por imagem
Doença de Parkinson/diagnóstico por imagem
Compostos Radiofarmacêuticos
Receptores Pré-Sinápticos/metabolismo
Tirosina/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Apomorfina/farmacologia
Descarboxilases de Aminoácido-L-Aromático/metabolismo
Modelos Animais de Doenças
Dopamina/metabolismo
Radioisótopos de Flúor
Processamento de Imagem Assistida por Computador
Masculino
Neostriado/diagnóstico por imagem
Oxidopamina
Doença de Parkinson Secundária/induzido quimicamente
Tomografia por Emissão de Pósitrons
Ratos
Ratos Sprague-Dawley
Comportamento Estereotipado/efeitos dos fármacos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorine Radioisotopes); 0 (Radiopharmaceuticals); 0 (Receptors, Presynaptic); 148613-12-1 (6-fluoro-3-tyrosine); 2C598205QX (fluorodopa F 18); 42HK56048U (Tyrosine); 63-84-3 (Dihydroxyphenylalanine); 8HW4YBZ748 (Oxidopamine); EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases); N21FAR7B4S (Apomorphine); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1111/jnc.14016


  6 / 914 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28279081
[Au] Autor:Sehara Y; Fujimoto KI; Ikeguchi K; Katakai Y; Ono F; Takino N; Ito M; Ozawa K; Muramatsu SI
[Ad] Endereço:1 Division of Genetic Therapeutics, Jichi Medical University , Yakushiji, Shimotsuke, Tochigi, Japan .
[Ti] Título:Persistent Expression of Dopamine-Synthesizing Enzymes 15 Years After Gene Transfer in a Primate Model of Parkinson's Disease.
[So] Source:Hum Gene Ther Clin Dev;28(2):74-79, 2017 Jun.
[Is] ISSN:2324-8645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Restoring dopamine production in the putamen through gene therapy is a straightforward strategy for ameliorating motor symptoms for Parkinson's disease (PD). In a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) toxicity-based primate model of PD, we previously showed the safety and efficacy of adeno-associated viral (AAV) vector-mediated gene delivery to the putamen of three dopamine-synthesizing enzymes (tyrosine hydroxylase [TH], aromatic l-amino acid decarboxylase [AADC], and guanosine triphosphate cyclohydrolase I [GCH]) up to 10 months postprocedure. Although three of four monkeys in this study have previously undergone postmortem analysis, one monkey was kept alive for 15 years after gene therapy to evaluate long-term effects. Here, we report that this monkey showed behavioral recovery in the right-side limb that remained unchanged for 15 years, at which time euthanasia was carried out owing to onset of senility. Immunohistochemistry of the postmortem brain from this monkey revealed persistent expression of TH, AADC, and GCH genes in the lesioned putamen. Transduced neurons were broadly distributed, with the estimated transduction region occupying 91% of the left postcommissural putamen. No signs of cytotoxicity or Lewy body pathology were observed in the AAV vector-injected putamen. This study provides evidence of long-term safety and efficacy of the triple-transduction method as a gene therapy for PD.
[Mh] Termos MeSH primário: Descarboxilases de Aminoácido-L-Aromático/genética
GTP Cicloidrolase/genética
Terapia Genética/efeitos adversos
Efeitos Adversos de Longa Duração/metabolismo
Intoxicação por MPTP/terapia
Tirosina 3-Mono-Oxigenase/genética
[Mh] Termos MeSH secundário: Animais
Descarboxilases de Aminoácido-L-Aromático/metabolismo
Dependovirus/genética
Dopamina/genética
Dopamina/metabolismo
GTP Cicloidrolase/metabolismo
Terapia Genética/métodos
Vetores Genéticos/administração & dosagem
Vetores Genéticos/genética
Efeitos Adversos de Longa Duração/diagnóstico
Macaca fascicularis
Putamen/metabolismo
Tirosina 3-Mono-Oxigenase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.14.16.2 (Tyrosine 3-Monooxygenase); EC 3.5.4.16 (GTP Cyclohydrolase); EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1089/humc.2017.010


  7 / 914 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28208655
[Au] Autor:De Masi L; Castaldo D; Pignone D; Servillo L; Facchiano A
[Ad] Endereço:Consiglio Nazionale delle Ricerche (CNR), Istituto di Biologia Agroambientale e Forestale (IBAF), via P. Castellino 111, 80131 Napoli, Italy. luigi.demasi@cnr.it.
[Ti] Título:Experimental Evidence and In Silico Identification of Tryptophan Decarboxylase in Citrus Genus.
[So] Source:Molecules;22(2), 2017 Feb 11.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Plant tryptophan decarboxylase (TDC) converts tryptophan into tryptamine, precursor of indolealkylamine alkaloids. The recent finding of tryptamine metabolites in plants leads to hypothesize the existence of TDC activity in this genus. Here, we report for the first time that, in x seedlings, deuterium labeled tryptophan is decarboxylated into tryptamine, from which successively deuterated , , -trimethyltryptamine is formed. These results give an evidence of the occurrence of the TDC activity and the successive methylation pathway of the tryptamine produced from the tryptophan decarboxylation. In addition, with the aim to identify the genetic basis for the presence of TDC, we carried out a sequence similarity search for TDC in the genomes using as a probe the TDC sequence reported for the plant . We analyzed the genomes of both and , available in public database, and identified putative protein sequences of aromatic l-amino acid decarboxylase. Similarly, 42 aromatic l-amino acid decarboxylase sequences from 23 plant species were extracted from public databases. Potential sequence signatures for functional TDC were then identified. With this research, we propose for the first time a putative protein sequence for TDC in the genus .
[Mh] Termos MeSH primário: Descarboxilases de Aminoácido-L-Aromático/genética
Citrus/enzimologia
Citrus/genética
[Mh] Termos MeSH secundário: Descarboxilases de Aminoácido-L-Aromático/metabolismo
Biologia Computacional/métodos
Ativação Enzimática
Perfilação da Expressão Gênica
Anotação de Sequência Molecular
Filogenia
Plântulas/genética
Plântulas/metabolismo
Triptaminas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tryptamines); 422ZU9N5TV (tryptamine); EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE


  8 / 914 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28166239
[Au] Autor:Ciesielska A; Samaranch L; San Sebastian W; Dickson DW; Goldman S; Forsayeth J; Bankiewicz KS
[Ad] Endereço:Department of Neurological Surgery, University of California San Francisco, San Francisco, CA, United States of America.
[Ti] Título:Depletion of AADC activity in caudate nucleus and putamen of Parkinson's disease patients; implications for ongoing AAV2-AADC gene therapy trial.
[So] Source:PLoS One;12(2):e0169965, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Parkinson's disease (PD), aromatic L-amino acid decarboxylase (AADC) is the rate-limiting enzyme in the conversion of L-DOPA (Sinemet) to dopamine (DA). Previous studies in PD animal models demonstrated that lesion of dopaminergic neurons is associated with profound loss of AADC activity in the striatum, blocking efficient conversion of L-DOPA to DA. Relatively few studies have directly analyzed AADC in PD brains. Thus, the aim of this study was to gain a better understanding of regional changes in AADC activity, DA, serotonin and their monoamine metabolites in the striatum of PD patients and experimentally lesioned animals (rat and MPTP-treated nonhuman primate, NHP). Striatal AADC activity was determined post mortem in neuropathologically confirmed PD subjects, animal models and controls. A regional analysis was performed for striatal AADC activity and monoamine levels in NHP tissue. Interestingly, analysis of putaminal AADC activity revealed that control human striatum contained much less AADC activity than rat and NHP striata. Moreover, a dramatic loss of AADC activity in PD striatum compared to controls was detected. In MPTP-treated NHP, caudate nucleus was almost as greatly affected as putamen, although mean DA turnover was higher in caudate nucleus. Similarly, DA and DA metabolites were dramatically reduced in different regions of PD brains, including caudate nucleus, whereas serotonin was relatively spared. After L-DOPA administration in MPTP-treated NHP, very poor conversion to DA was detected, suggesting that AADC in NHP nigrostriatal fibers is mainly responsible for L-DOPA to DA conversion. These data support further the rationale behind viral gene therapy with AAV2-hAADC to restore AADC levels in putamen and suggest further the advisability of expanding vector delivery to include coverage of anterior putamen and the caudate nucleus.
[Mh] Termos MeSH primário: Descarboxilases de Aminoácido-L-Aromático/metabolismo
Núcleo Caudado/metabolismo
Doença de Parkinson/metabolismo
Putamen/metabolismo
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Animais
Descarboxilases de Aminoácido-L-Aromático/genética
Corpo Estriado/metabolismo
Dependovirus/genética
Modelos Animais de Doenças
Dopamina/metabolismo
Ativação Enzimática
Feminino
Terapia Genética
Vetores Genéticos/genética
Seres Humanos
Levodopa/metabolismo
Levodopa/uso terapêutico
Macaca mulatta
Masculino
Doença de Parkinson/genética
Doença de Parkinson/terapia
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
46627O600J (Levodopa); EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170207
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169965


  9 / 914 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:28100326
[Au] Autor:Zhu J; Yu F
[Ad] Endereço:Department of Pediatric Endocrine and Genetic Metabolic Disease, Maternal and Children's Hospital of Hubei Province, Wuhan 430070, China. okyufei@sohu.com.
[Ti] Título:[Feeding difficulty and developmental delay for 8 months and nystagmus for 4 months in an infant].
[So] Source:Zhongguo Dang Dai Er Ke Za Zhi;19(1):68-72, 2017 Jan.
[Is] ISSN:1008-8830
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Aromatic L-amino acid decarboxylase (AADC) deficiency is a rare autosomal recessive hereditary disease and is a congenital metabolic disorder of neurotransmitter biosynthesis. It is mainly manifested as hypotonia, oculogyric crisis, autonomic dysfunction, and developmental delay. This article reports a boy manifested as delayed motor development, hypotonia, and oculogyric crisis. Gene screening for metabolic disorders revealed new compound heterozygous mutations, c.1063dupA (p.I355fs) and c.250A>C (p.S84R), in the exon of DDC gene. The boy had a significant increase in 3-O-methyldopa as measured by dried blood spot. Therefore, he was diagnosed with AADC deficiency. After treatment with the dopamine receptor agonist pramipexole dihydrochloride, the catechol-O-methyltransferase inhibitor entacapone, and vitamin B6, the boy showed mild improvements in hypotonia, blepharoptosis, and oculogyric crisis. Clinical physicians should enhance their ability for identifying AADC deficiency, so as to facilitate early diagnosis and treatment of this disorder. Genetic counseling for birth health and prenatal diagnosis should be performed for parents in need.
[Mh] Termos MeSH primário: Erros Inatos do Metabolismo dos Aminoácidos/complicações
Descarboxilases de Aminoácido-L-Aromático/deficiência
Deficiências do Desenvolvimento/etiologia
Transtornos da Alimentação e da Ingestão de Alimentos/etiologia
Nistagmo Patológico/etiologia
[Mh] Termos MeSH secundário: Seres Humanos
Lactente
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE


  10 / 914 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27940130
[Au] Autor:Akiyama T; Hayashi Y; Hanaoka Y; Shibata T; Akiyama M; Nakamura K; Tsuyusaki Y; Kubota M; Yoshinaga H; Kobayashi K
[Ad] Endereço:Department of Child Neurology, Okayama University Hospital, Okayama, Japan. Electronic address: takiyama@okayama-u.ac.jp.
[Ti] Título:Simultaneous measurement of monoamine metabolites and 5-methyltetrahydrofolate in the cerebrospinal fluid of children.
[So] Source:Clin Chim Acta;465:5-10, 2017 Feb.
[Is] ISSN:1873-3492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: We describe a new method for simultaneous measurement of monoamine metabolites (3-O-methyldopa [3-OMD], 3-methoxy-4-hydroxyphenylethyleneglycol [MHPG], 5-hydroxyindoleacetic acid [5-HIAA], and homovanillic acid [HVA]) and 5-methyltetrahydrofolate (5-MTHF) and its use on cerebrospinal fluid (CSF) samples from pediatric patients. METHODS: Monoamine metabolites and 5-MTHF were measured by high-performance liquid chromatography with fluorescence detection. CSF samples were prospectively collected from children according to a standardized collection protocol in which the first 1-ml fraction was used for analysis. RESULTS: Monoamine metabolites and 5-MTHF were separated within 10min. They showed linearity from the limit of detection to 1024nmol/l. The limit of quantification of each metabolite was sufficiently low for the CSF sample assay. In 42 CSF samples after excluding cases with possibly altered neurotransmitter profiles, the concentrations of 3-OMD, MHPG, 5-HIAA, HVA, and 5-MTHF showed significant age dependence and their ranges were comparable with the reference values in the literature. The metabolite profiles of aromatic l-amino acid decarboxylase deficiency, Segawa disease, and folate receptor α defect by this method were compatible with those in the literature. CONCLUSIONS: This method is a simple means of measuring CSF monoamine metabolites and 5-MTHF, and is especially useful for laboratories not equipped with electrochemical detectors.
[Mh] Termos MeSH primário: Di-Hidroxifenilalanina/análogos & derivados
Ácido Homovanílico/líquido cefalorraquidiano
Ácido Hidroxi-Indolacético/líquido cefalorraquidiano
Metoxi-Hidroxifenilglicol/líquido cefalorraquidiano
Tetra-Hidrofolatos/líquido cefalorraquidiano
[Mh] Termos MeSH secundário: Descarboxilases de Aminoácido-L-Aromático/líquido cefalorraquidiano
Descarboxilases de Aminoácido-L-Aromático/deficiência
Cromatografia Líquida de Alta Pressão/métodos
Di-Hidroxifenilalanina/líquido cefalorraquidiano
Distúrbios Distônicos/líquido cefalorraquidiano
Fluorescência
Receptor 1 de Folato/líquido cefalorraquidiano
Receptor 1 de Folato/deficiência
Receptor 1 de Folato/genética
Seres Humanos
Limite de Detecção
Distrofias Neuroaxonais/líquido cefalorraquidiano
Valores de Referência
Reprodutibilidade dos Testes
Tirosina/análogos & derivados
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (FOLR1 protein, human); 0 (Folate Receptor 1); 0 (Tetrahydrofolates); 42HK56048U (Tyrosine); 534-82-7 (Methoxyhydroxyphenylglycol); 54-16-0 (Hydroxyindoleacetic Acid); 63-84-3 (Dihydroxyphenylalanine); EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases); EC 4.1.1.28 (DDC protein, human); TYK22LML8F (5-methyltetrahydrofolate); V3O7J20DWN (3-methoxytyrosine); X77S6GMS36 (Homovanillic Acid)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE



página 1 de 92 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde