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Pesquisa : D08.811.682.113 [Categoria DeCS]
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[PMID]:28188921
[Au] Autor:Menezes RA; Pimentel C; Silva AR; Amaral C; Merhej J; Devaux F; Rodrigues-Pousada C
[Ad] Endereço:Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
[Ti] Título:Mediator, SWI/SNF and SAGA complexes regulate Yap8-dependent transcriptional activation of ACR2 in response to arsenate.
[So] Source:Biochim Biophys Acta;1860(4):472-481, 2017 04.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Response to arsenic stress in Saccharomyces cerevisiae is orchestrated by the regulatory protein Yap8, which mediates transcriptional activation of ACR2 and ACR3. This study contributes to the state of art knowledge of the molecular mechanisms underlying yeast stress response to arsenate as it provides the genetic and biochemical evidences that Yap8, through cysteine residues 132, 137, and 274, is the sensor of presence of arsenate in the cytosol. Moreover, it is here reported for the first time the essential role of the Mediator complex in the transcriptional activation of ACR2 by Yap8. Based on our data, we propose an order-of-function map to recapitulate the sequence of events taking place in cells injured with arsenate. Modification of the sulfhydryl state of these cysteines converts Yap8 in its activated form, triggering the recruitment of the Mediator complex to the ACR2/ACR3 promoter, through the interaction with the tail subunit Med2. The Mediator complex then transfers the regulatory signals conveyed by Yap8 to the core transcriptional machinery, which culminates with TBP occupancy, ACR2 upregulation and cell adaptation to arsenate stress. Additional co-factors are required for the transcriptional activation of ACR2 by Yap8, particularly the nucleosome remodeling activity of SWI/SNF and SAGA complexes.
[Mh] Termos MeSH primário: Arseniato Redutases/genética
Arseniatos/toxicidade
Fatores de Transcrição de Zíper de Leucina Básica/metabolismo
Complexo Mediador/metabolismo
Complexos Multiproteicos/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/metabolismo
Ativação Transcricional/genética
[Mh] Termos MeSH secundário: Arseniato Redutases/metabolismo
Fatores de Transcrição de Zíper de Leucina Básica/química
Cisteína/metabolismo
Regiões Promotoras Genéticas
Ligação Proteica
Subunidades Proteicas/metabolismo
Proteínas de Saccharomyces cerevisiae/química
Proteínas de Saccharomyces cerevisiae/genética
Estresse Fisiológico/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (ARR1 protein, S cerevisiae); 0 (Arsenates); 0 (Basic-Leucine Zipper Transcription Factors); 0 (Mediator Complex); 0 (Multiprotein Complexes); 0 (Protein Subunits); 0 (Saccharomyces cerevisiae Proteins); EC 1.20.- (ARR2 protein, S cerevisiae); EC 1.20.- (Arsenate Reductases); K848JZ4886 (Cysteine); N7CIZ75ZPN (arsenic acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170212
[St] Status:MEDLINE


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[PMID]:28054716
[Au] Autor:Nookongbut P; Kantachote D; Krishnan K; Megharaj M
[Ad] Endereço:Faculty of Science, Department of Microbiology, Prince of Songkla University, Hat Yai, Thailand.
[Ti] Título:Arsenic resistance genes of As-resistant purple nonsulfur bacteria isolated from As-contaminated sites for bioremediation application.
[So] Source:J Basic Microbiol;57(4):316-324, 2017 Apr.
[Is] ISSN:1521-4028
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This study aimed to identify arsenic resistant mechanisms in As-resistant purple nonsulfur bacteria (PNSB) by screening them for presence of As-resistance genes and related enzymes. Resistance to As(III) and As(V) of four As-resistant PNSB determined in terms of median inhibition concentration (IC values) were in the order of strains Rhodopseudomonas palustris C1 > R. palustris AB3 > Rubrivivax benzoatilyticus C31 > R. palustris L28 which corresponded to the presence of As-resistance genes in these bacteria. The strain C1 showed all As-marker genes; arsC, arsM, aioA, and acr3, while aioA was not detected in strain AB3. Strains C31 and L28 had only Arsenite-transporter gene, acr3. Translation of all these detected gene sequences of strain C1 to amino acid sequences showed that these proteins have vicinal cysteine; Cys126, Cys105, and Cys178 of Acr3, ArsC, AioA, respectively. Tertiary structure of proteins Acr3, ArsC, AioA, and ArsM showed strain C1 exhibits the high activities of arsenite oxidase and arsenate reductase enzymes that are encoded by aioA and arsC genes, respectively. Moreover, strain C1 with arsM gene produced volatile-methylated As-compounds; monomethylarsonic acid (MMA), dimethylarsenic acid (DMA), and arsenobetaine (AsB) in the presence of either As(III) or As(V). In conclusion, the strain C1 has great potential for its application in bioremediation of As-contaminated sites.
[Mh] Termos MeSH primário: Arseniatos/farmacologia
Arsênico/metabolismo
Arsenitos/farmacologia
Genes Bacterianos
Estrutura Terciária de Proteína
Rhodospirillaceae/efeitos dos fármacos
Rhodospirillaceae/genética
[Mh] Termos MeSH secundário: Arseniato Redutases/metabolismo
Arseniatos/metabolismo
Arsenicais/metabolismo
Arsenitos/metabolismo
Biodegradação Ambiental
Ácido Cacodílico/metabolismo
Regulação Bacteriana da Expressão Gênica
Oxirredutases/metabolismo
Rodopseudomonas/efeitos dos fármacos
Rodopseudomonas/genética
Rodopseudomonas/isolamento & purificação
Rhodospirillaceae/isolamento & purificação
Rhodospirillaceae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arsenates); 0 (Arsenicals); 0 (Arsenites); AJ2HL7EU8K (Cacodylic Acid); EC 1.- (Oxidoreductases); EC 1.16.- (arsenite oxidase); EC 1.20.- (Arsenate Reductases); J37VJ5709S (monomethylarsonic acid); N712M78A8G (Arsenic); UWC1LS4V3I (arsenobetaine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.1002/jobm.201600584


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[PMID]:27933331
[Au] Autor:Yin X; Wang L; Liu Y; Jiang T; Gao J
[Ad] Endereço:Jinan Research Academy of Environmental Sciences, Jinan, 250014, China.
[Ti] Título:Characterization of Arsenic Biotransformation by a Typical Bryophyte Physcomitrella patens.
[So] Source:Bull Environ Contam Toxicol;98(2):251-256, 2017 Feb.
[Is] ISSN:1432-0800
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arsenic (As) is a ubiquitous environmental toxin that has created catastrophic human health and environmental problems around world. Physcomitrella patens is a potential model plant for the study of environmental monitoring, which exists in all kinds of ecosystems. In this study, arsenic metabolism was investigated by this moss. When supplied with different levels of arsenate (50, 100, 200 µmol/L) for a 4-week period, the total arsenic concentrations were up to 231.4-565.4 mg/kg DW in this moss. Arsenite concentration increased with increasing external arsenate concentrations, the proportion was up to 25.1-36.8% of the total As. An arsenate reductase, PpACR2, was identified and functionally characterized. Heterologous expression of PpACR2 in an As(V)-sensitive strain WC3110 (ΔarsC) of Escherichia coli conferred As(V) resistance. Purified PpACR2 protein exhibited the arsenate reductase activity. Given its powerful As accumulation ability, the bryophyte could be exploited in bioremediation of As-contaminated environments.
[Mh] Termos MeSH primário: Arseniatos/metabolismo
Arsênico/metabolismo
Arsenitos/metabolismo
Bryopsida/metabolismo
[Mh] Termos MeSH secundário: Arseniato Redutases/metabolismo
Biotransformação
Bryopsida/enzimologia
Escherichia coli/metabolismo
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arsenates); 0 (Arsenites); EC 1.20.- (Arsenate Reductases); N5509X556J (arsenite); N712M78A8G (Arsenic); N7CIZ75ZPN (arsenic acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1007/s00128-016-1997-y


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[PMID]:27707908
[Au] Autor:Politi J; Spadavecchia J; Fiorentino G; Antonucci I; De Stefano L
[Ad] Endereço:Institute for Microelectronics and Microsystems, Unit of Naples-National Research Council, via P. Castellino 111, 80127 Naples, Italy.
[Ti] Título:Arsenate reductase from Thermus thermophilus conjugated to polyethylene glycol-stabilized gold nanospheres allow trace sensing and speciation of arsenic ions.
[So] Source:J R Soc Interface;13(123), 2016 Oct.
[Is] ISSN:1742-5662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Water sources pollution by arsenic ions is a serious environmental problem all around the world. Arsenate reductase enzyme (TtArsC) from Thermus thermophilus extremophile bacterium, naturally binds arsenic ions, As(V) and As (III), in aqueous solutions. In this research, TtArsC enzyme adsorption onto hybrid polyethylene glycol-stabilized gold nanoparticles (AuNPs) was studied at different pH values as an innovative nanobiosystem for metal concentration monitoring. Characterizations were performed by UV/Vis and circular dichroism spectroscopies, TEM images and in terms of surface charge changes. The molecular interaction between arsenic ions and the TtArsC-AuNPs nanobiosystem was also monitored at all pH values considered by UV/Vis spectroscopy. Tests performed revealed high sensitivities and limits of detection equal to 10 ± 3 M and 7.7 ± 0.3 M for As(III) and As(V), respectively.
[Mh] Termos MeSH primário: Arseniato Redutases/química
Arsênico/análise
Proteínas de Bactérias/química
Técnicas Biossensoriais/métodos
Ouro/química
Nanopartículas Metálicas/química
Polietilenoglicóis/química
Thermus thermophilus/enzimologia
[Mh] Termos MeSH secundário: Enzimas Imobilizadas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Enzymes, Immobilized); 30IQX730WE (Polyethylene Glycols); 7440-57-5 (Gold); EC 1.20.- (Arsenate Reductases); N712M78A8G (Arsenic); U076Q6Q621 (polyethylene glycol 1000)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161007
[St] Status:MEDLINE


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[PMID]:27702843
[Au] Autor:Shi S; Wang T; Chen Z; Tang Z; Wu Z; Salt DE; Chao DY; Zhao FJ
[Ad] Endereço:State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China (S.S., Z.T., F.-J.Z.).
[Ti] Título:OsHAC1;1 and OsHAC1;2 Function as Arsenate Reductases and Regulate Arsenic Accumulation.
[So] Source:Plant Physiol;172(3):1708-1719, 2016 Nov.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rice is a major dietary source of the toxic metalloid arsenic (As). Reducing its accumulation in rice (Oryza sativa) grain is of critical importance to food safety. Rice roots take up arsenate and arsenite depending on the prevailing soil conditions. The first step of arsenate detoxification is its reduction to arsenite, but the enzyme(s) catalyzing this reaction in rice remains unknown. Here, we identify OsHAC1;1 and OsHAC1;2 as arsenate reductases in rice. OsHAC1;1 and OsHAC1;2 are able to complement an Escherichia coli mutant lacking the endogenous arsenate reductase and to reduce arsenate to arsenite. OsHAC1:1 and OsHAC1;2 are predominantly expressed in roots, with OsHAC1;1 being abundant in the epidermis, root hairs, and pericycle cells while OsHAC1;2 is abundant in the epidermis, outer layers of cortex, and endodermis cells. Expression of the two genes was induced by arsenate exposure. Knocking out OsHAC1;1 or OsHAC1;2 decreased the reduction of arsenate to arsenite in roots, reducing arsenite efflux to the external medium. Loss of arsenite efflux was also associated with increased As accumulation in shoots. Greater effects were observed in a double mutant of the two genes. In contrast, overexpression of either OsHAC1;1 or OsHAC1;2 increased arsenite efflux, reduced As accumulation, and enhanced arsenate tolerance. When grown under aerobic soil conditions, overexpression of either OsHAC1;1 or OsHAC1;2 also decreased As accumulation in rice grain, whereas grain As increased in the knockout mutants. We conclude that OsHAC1;1 and OsHAC1;2 are arsenate reductases that play an important role in restricting As accumulation in rice shoots and grain.
[Mh] Termos MeSH primário: Arseniato Redutases/metabolismo
Arsênico/metabolismo
Oryza/enzimologia
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Adaptação Fisiológica/efeitos dos fármacos
Adaptação Fisiológica/genética
Arsênico/toxicidade
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Técnicas de Inativação de Genes
Especiação Genética
Proteínas de Fluorescência Verde/metabolismo
Mutação/genética
Oryza/efeitos dos fármacos
Oryza/genética
Oryza/crescimento & desenvolvimento
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
Brotos de Planta/efeitos dos fármacos
Brotos de Planta/metabolismo
Plantas Geneticamente Modificadas
Proteínas Recombinantes de Fusão/metabolismo
Solo
Frações Subcelulares/efeitos dos fármacos
Frações Subcelulares/metabolismo
Xilema/efeitos dos fármacos
Xilema/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Recombinant Fusion Proteins); 0 (Soil); 147336-22-9 (Green Fluorescent Proteins); EC 1.20.- (Arsenate Reductases); N712M78A8G (Arsenic)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


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[PMID]:27565307
[Au] Autor:Kumari N; Jagadevan S
[Ad] Endereço:Department of Environmental Science and Engineering, Indian School of Mines, Dhanbad, Jharkhand, 826004, India.
[Ti] Título:Genetic identification of arsenate reductase and arsenite oxidase in redox transformations carried out by arsenic metabolising prokaryotes - A comprehensive review.
[So] Source:Chemosphere;163:400-412, 2016 Nov.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Arsenic (As) contamination in water is a cause of major concern to human population worldwide, especially in Bangladesh and West Bengal, India. Arsenite (As(III)) and arsenate (As(V)) are the two common forms in which arsenic exists in soil and groundwater, the former being more mobile and toxic. A large number of arsenic metabolising microorganisms play a crucial role in microbial transformation of arsenic between its different states, thus playing a key role in remediation of arsenic contaminated water. This review focuses on advances in biochemical, molecular and genomic developments in the field of arsenic metabolising bacteria - covering recent developments in the understanding of structure of arsenate reductase and arsenite oxidase enzymes, their gene and operon structures and their mechanism of action. The genetic and molecular studies of these microbes and their proteins may lead to evolution of successful strategies for effective implementation of bioremediation programs.
[Mh] Termos MeSH primário: Arseniato Redutases/genética
Arsênico/metabolismo
Bactérias/metabolismo
Proteínas de Bactérias/genética
Poluentes Ambientais/metabolismo
Oxirredutases/genética
[Mh] Termos MeSH secundário: Arseniato Redutases/metabolismo
Arseniatos/metabolismo
Arsenitos/metabolismo
Bactérias/genética
Proteínas de Bactérias/metabolismo
Biodegradação Ambiental
Biotransformação
Oxirredução
Oxirredutases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Arsenates); 0 (Arsenites); 0 (Bacterial Proteins); 0 (Environmental Pollutants); EC 1.- (Oxidoreductases); EC 1.16.- (arsenite oxidase); EC 1.20.- (Arsenate Reductases); N5509X556J (arsenite); N712M78A8G (Arsenic); N7CIZ75ZPN (arsenic acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160828
[St] Status:MEDLINE


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[PMID]:27174139
[Au] Autor:Verma PK; Verma S; Meher AK; Pande V; Mallick S; Bansiwal AK; Tripathi RD; Dhankher OP; Chakrabarty D
[Ad] Endereço:Genetics and Molecular Biology Division, CSIR-National Botanical Research Institute, India; Department of Biotechnology, Kumaun University, India.
[Ti] Título:Overexpression of rice glutaredoxins (OsGrxs) significantly reduces arsenite accumulation by maintaining glutathione pool and modulating aquaporins in yeast.
[So] Source:Plant Physiol Biochem;106:208-17, 2016 Sep.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Arsenic (As) is an acute poison and class I carcinogen, can cause a serious health risk. Staple crops like rice are the primary source of As contamination in human food. Rice grown on As contaminated areas accumulates higher As in their edible parts. Based on our previous transcriptome data, two rice glutaredoxins (OsGrx_C7 and OsGrx_C2.1) were identified that showed up-regulated expression during As stress. Here, we report OsGrx_C7 and OsGrx_C2.1 from rice involved in the regulation of intracellular arsenite (AsIII). To elucidate the mechanism of OsGrx mediated As tolerance, both OsGrxs were cloned and expressed in Escherichia coli (Δars) and Saccharomyces cerevisiae mutant strains (Δycf1, Δacr3). The expression of OsGrxs increased As tolerance in E. coli (Δars) mutant strain (up to 4 mM AsV and up to 0.6 mM AsIII). During AsIII exposure, S. cerevisiae (Δacr3) harboring OsGrx_C7 and OsGrx_C2.1 have lower intracellular AsIII accumulation (up to 30.43% and 24.90%, respectively), compared to vector control. Arsenic accumulation in As-sensitive S. cerevisiae mutant (Δycf1) also reduced significantly on exposure to inorganic As. The expression of OsGrxs in yeast maintained intracellular GSH pool and increased extracellular GSH concentration. Purified OsGrxs displays in vitro GSH-disulfide oxidoreductase, glutathione reductase and arsenate reductase activities. Also, both OsGrxs are involved in AsIII extrusion by altering the Fps1 transcripts in yeast and protect the cell by maintaining cellular GSH pool. Thus, our results strongly suggest that OsGrxs play a crucial role in the maintenance of the intracellular GSH pool and redox status of the cell during both AsV and AsIII stress and might be involved in regulating intracellular AsIII levels by modulation of aquaporin expression and functions.
[Mh] Termos MeSH primário: Aquaporinas/metabolismo
Arsenitos/metabolismo
Glutarredoxinas/metabolismo
Glutationa/metabolismo
Oryza/metabolismo
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Arseniato Redutases/metabolismo
Transporte Biológico
Genes de Plantas
Teste de Complementação Genética
Glutationa Redutase/metabolismo
Mutação/genética
Oryza/genética
Fenótipo
Proteína Dissulfeto Redutase (Glutationa)/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aquaporins); 0 (Arsenites); 0 (Glutaredoxins); 0 (RNA, Messenger); 0 (Saccharomyces cerevisiae Proteins); EC 1.20.- (Arsenate Reductases); EC 1.8.1.7 (Glutathione Reductase); EC 1.8.4.2 (Protein Disulfide Reductase (Glutathione)); GAN16C9B8O (Glutathione); N5509X556J (arsenite)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160514
[St] Status:MEDLINE


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[PMID]:27061371
[Au] Autor:Begum MC; Islam MS; Islam M; Amin R; Parvez MS; Kabir AH
[Ad] Endereço:Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh.
[Ti] Título:Biochemical and molecular responses underlying differential arsenic tolerance in rice (Oryza sativa L.).
[So] Source:Plant Physiol Biochem;104:266-77, 2016 Jul.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The arsenic (As) is a toxic element causing major health concern worldwide. Arsenate stress caused no significant reduction in growth parameters and shoot electrolyte leakage but showed increased root arsenate reductase activity along with relatively lower root As content and shoot translocation rate in As-tolerant BRRI 33 than in As-sensitive BRRI 51. It indicates that As inhibition and tolerance mechanisms are driven by root responses. Interestingly, As stress showed consistent decrease in phosphate content and expression of phosphate transporters (OsPT8, OsPT4, OsPHO1;2) under both high and low phosphate conditions in roots of BRRI 33, suggesting that limiting phosphate transport mainly mediated by OsPHO1;2 directs less As accumulation in BRRI 33. Further, BRRI 33 showed simultaneous increase in OsPCS1 (phytochelatin synthase) expression and phytochelatins (PCs) content in roots under As exposure supporting the hypothesis that root As sequestration acts as 'firewall system' in limiting As translocation in shoots. Furthermore, increased CAT, POD, SOD, GR, along with elevated glutathione, methionine, cysteine and proline suggests that strong antioxidant defense plays integral part to As tolerance in BRRI 33. Again, BRRI 33 self-grafts and plants having BRRI 33 rootstock combined with BRRI 51 scion had no adverse effect on morphological parameters but showed reduced As translocation rate, increased root arsenate reductase activity, shoot PC synthesis and root OsPHO1;2 expression due to As stress. It confirms that signal driving As tolerance mechanisms is generated in the roots. These findings can be implemented for As detoxification and As-free transgenic rice production for health safety.
[Mh] Termos MeSH primário: Adaptação Fisiológica/efeitos dos fármacos
Arsênico/toxicidade
Oryza/genética
Oryza/fisiologia
[Mh] Termos MeSH secundário: Adaptação Fisiológica/genética
Aminoácidos/metabolismo
Antioxidantes/metabolismo
Arseniato Redutases/metabolismo
Transporte Biológico/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Oryza/anatomia & histologia
Oryza/efeitos dos fármacos
Fenótipo
Fitoquelatinas/metabolismo
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/genética
Raízes de Plantas/metabolismo
Brotos de Planta/efeitos dos fármacos
Brotos de Planta/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Espectrofotometria Atômica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Antioxidants); 0 (Plant Proteins); 98726-08-0 (Phytochelatins); EC 1.20.- (Arsenate Reductases); N712M78A8G (Arsenic)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160411
[St] Status:MEDLINE


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[PMID]:26915994
[Au] Autor:Bandyopadhyay S; Das SK
[Ad] Endereço:Department of Biotechnology, Institute of Life Sciences, Nalco Square, Bhubaneswar, 751023, India.
[Ti] Título:Functional analysis of ars gene cluster of Pannonibacter indicus strain HT23(T) (DSM 23407(T)) and identification of a proline residue essential for arsenate reductase activity.
[So] Source:Appl Microbiol Biotechnol;100(7):3235-44, 2016 Apr.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Arsenic is a naturally occurring ubiquitous highly toxic metalloid. In this study, we have identified ars gene cluster in Pannonibacter indicus strain HT23(T) (DSM 23407(T)), responsible for reduction of toxic pentavalent arsenate. The ars gene cluster is comprised of four non-overlapping open reading frames (ORFs) encoding a transcriptional regulator (ArsR), a low molecular weight protein tyrosine phosphatases (LMW-PTPase) with hypothetical function, an arsenite efflux pump (Acr3), and an arsenate reductase (ArsC). Heterologous expression of arsenic inducible ars gene cluster conferred arsenic resistance to Escherichia coli ∆ars mutant strain AW3110. The recombinant ArsC was purified and assayed. Site-directed mutagenesis was employed to ascertain the role of specific amino acids in ArsC catalysis. Pro94X (X = Ala, Arg, Cys, and His) amino acid substitutions led to enzyme inactivation. Circular dichroism spectra analysis suggested Pro94 as an essential amino acid for enzyme catalytic activity as it is indispensable for optimum protein folding in P. indicus Grx-coupled ArsC.
[Mh] Termos MeSH primário: Arseniato Redutases/genética
Proteínas de Bactérias/genética
Regulação Bacteriana da Expressão Gênica
Família Multigênica
Prolina/química
Rhodobacteraceae/genética
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Arseniato Redutases/metabolismo
Arsênico/metabolismo
Proteínas de Bactérias/metabolismo
Clonagem Molecular
Escherichia coli/genética
Escherichia coli/metabolismo
Cinética
Mutagênese Sítio-Dirigida
Fases de Leitura Aberta
Óperon
Oxirredução
Plasmídeos/química
Plasmídeos/metabolismo
Prolina/metabolismo
Dobramento de Proteína
Proteínas Tirosina Fosfatases/genética
Proteínas Tirosina Fosfatases/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Rhodobacteraceae/enzimologia
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Recombinant Proteins); 9DLQ4CIU6V (Proline); EC 1.20.- (Arsenate Reductases); EC 3.1.3.48 (Protein Tyrosine Phosphatases); N712M78A8G (Arsenic)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160227
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-016-7390-2


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[PMID]:26865328
[Au] Autor:Jain R; Jha S; Mahatma MK; Jha A; Kumar GN
[Ad] Endereço:a Department of Plant Molecular Biology and Biotechnology , ASPEE College of Horticulture and Forestry, Navsari Agricultural University , Navsari , Gujarat , India.
[Ti] Título:Characterization of arsenite tolerant Halomonas sp. Alang-4, originated from heavy metal polluted shore of Gulf of Cambay.
[So] Source:J Environ Sci Health A Tox Hazard Subst Environ Eng;51(6):478-86, 2016.
[Is] ISSN:1532-4117
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Arsenite [As(III)]-oxidizing bacteria were isolated from heavy metal contaminated shore of Gulf of Cambay at Alang, India. The most efficient bacterial strain Alang-4 could tolerate up to 15 mM arsenite [As(III)] and 200 mM of arsenate [As(V)]. Its 16S rRNA gene sequence was 99% identical to the 16S rRNA genes of genus Halomonas (Accession no. HQ659187). Arsenite oxidase enzyme localized on membrane helped in conversion of As(III) to As(V). Arsenite transporter genes (arsB, acr3(1) and acr3(2)) assisted in extrusion of arsenite from Halomonas sp. Alang-4. Generation of ROS in response to arsenite stress was alleviated by higher activities of catalase, ascorbate peroxidase, superoxide dismutase and glutathione S-transferase enzymes. Down-regulation in the specific activities of nearly all dehydrogenases of carbon assimilatory pathway viz., glucose-6-phosphate, pyruvate, α-ketoglutarate, isocitrate and malate dehydrogenases, was observed in presence of As(III), whereas, the specific activities of phosphoenol pyruvate carboxylase, pyruvate carboxylase and isocitrate lyase enzymes were found to increase two times in As(III) treated cells. The results suggest that in addition to efficient ars operon, alternative pathways of carbon utilization exist in the marine bacterium Halomonas sp. Alang-4 to overcome the toxic effects of arsenite on its dehydrogenase enzymes.
[Mh] Termos MeSH primário: Arseniato Redutases/metabolismo
Arsenitos/metabolismo
Halomonas/química
Metais Pesados/metabolismo
Oxirredutases/metabolismo
Água do Mar/química
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Arseniato Redutases/genética
Monitoramento Ambiental
Halomonas/genética
Índia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arsenites); 0 (Metals, Heavy); EC 1.- (Oxidoreductases); EC 1.20.- (Arsenate Reductases)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160212
[St] Status:MEDLINE
[do] DOI:10.1080/10934529.2015.1128717



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