[PMID]: | 26968548 |
[Au] Autor: | Yeung KR; Chiu CL; Pidsley R; Makris A; Hennessy A; Lind JM |
[Ad] Endereço: | School of Medicine, Western Sydney University, Sydney, Australia; |
[Ti] Título: | DNA methylation profiles in preeclampsia and healthy control placentas. |
[So] Source: | Am J Physiol Heart Circ Physiol;310(10):H1295-303, 2016 May 15. |
[Is] ISSN: | 1522-1539 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Preeclampsia is a hypertensive disorder of pregnancy that affects 3-5% of all pregnancies. There is evidence to suggest that epigenetic mechanisms, such as DNA methylation, play a role in placental development and function. This study compared DNA methylation profiles of placentas from preeclampsia-affected pregnancies with placentas from healthy pregnancies to identify gene-specific changes in DNA methylation that may contribute to the development of preeclampsia. The methylation status of eight placental biopsies taken from preeclampsia-affected and 16 healthy pregnancies was analyzed using the Illumina Infinium Methylation 450 BeadChip array. Bisulfite pyrosequencing was used to confirm regions found to be differentially methylated between preeclampsia and healthy placentas. A total of 303 differentially methylated regions, 214 hypermethylated and 89 hypomethylated, between preeclampsia cases and controls were identified, after adjusting for gestational age (adjusted P < 0.05). Functional annotation found cell adhesion, wingless type MMTV Integration Site family member 2 (Wnt) signaling pathway, and regulation of transcription were significantly enriched in these gene regions. Hypermethylation of WNT2, sperm equatorial segment protein (SPESP1), NADPH oxidase 5 (NOX5), and activated leukocyte cell adhesion molecule (ALCAM) in preeclampsia placentas was confirmed with pyrosequencing. This study found differences in methylation in gene regions involved in cell signaling (WNT2), fertilization and implantation (SPESP1), reactive oxygen species signaling (NOX5), and cell adhesion (ALCAM). These results build on recently published studies that have reported significant differences in DNA methylation in preeclampsia placentas. |
[Mh] Termos MeSH primário: |
Metilação de DNA Epigênese Genética Placenta/química Pré-Eclâmpsia/genética
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[Mh] Termos MeSH secundário: |
Adulto Antígenos CD/genética Proteínas de Transporte/genética Estudos de Casos e Controles Moléculas de Adesão Celular Neuronais/genética Epigenômica/métodos Feminino Proteínas Fetais/genética Perfilação da Expressão Gênica Predisposição Genética para Doença Estudo de Associação Genômica Ampla Seres Humanos Proteínas de Membrana/genética NADPH Oxidase 5 NADPH Oxidases/genética Pré-Eclâmpsia/diagnóstico Gravidez Proteínas de Plasma Seminal/genética Transcrição Genética Proteína Wnt2/genética
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (ALCAM protein, human); 0 (Antigens, CD); 0 (Carrier Proteins); 0 (Cell Adhesion Molecules, Neuronal); 0 (Fetal Proteins); 0 (Membrane Proteins); 0 (SPESP1 protein, human); 0 (Seminal Plasma Proteins); 0 (WNT2 protein, human); 0 (Wnt2 Protein); EC 1.6.3.- (NADPH Oxidase 5); EC 1.6.3.- (NADPH Oxidases); EC 1.6.3.- (NOX5 protein, human) |
[Em] Mês de entrada: | 1706 |
[Cu] Atualização por classe: | 171116 |
[Lr] Data última revisão:
| 171116 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 160313 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1152/ajpheart.00958.2015 |
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