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  1 / 16729 MEDLINE  
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[PMID]:29318252
[Au] Autor:Carvalho ATP; Dourado DFAR; Skvortsov T; de Abreu M; Ferguson LJ; Quinn DJ; Moody TS; Huang M
[Ad] Endereço:School of Chemistry and Chemical Engineering, Queen's University, David Keir Building, Stranmillis Road, Belfast BT9 5AG, Northern Ireland, UK. m.huang@qub.ac.uk.
[Ti] Título:Spatial requirement for PAMO for transformation of non-native linear substrates.
[So] Source:Phys Chem Chem Phys;20(4):2558-2570, 2018 Jan 24.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phenylacetone monooxygenase is the most stable and thermo-tolerant member of the Baeyer-Villiger monooxygenases family, and therefore it is an ideal candidate for the synthesis of industrially relevant ester or lactone compounds. However, its limited substrate scope has largely limited its industrial applications. Linear substrates are interesting from an industrial point of view, it is thus necessary to identify the essential spatial requirement for achieving high conversions for non-native linear substrates. Here using molecular dynamics simulations, we compared the conversion of a non-native linear substrate 2-octanone and the native substrate phenylacetone, catalyzed by the WT enzyme and a quadruple variant P253F/G254A/R258M/L443F that exhibits significantly improved activity towards 2-octanone. We uncovered that a remarkable movement of L289 is crucial for a reshaping of the active site of the quadruple variant so as to prevent the aliphatic substrate from moving away from the C4a-peroxyflavin, thus enabling it to keep a catalytically relevant pose during the oxygenation process. By performing steady-state kinetic analysis of two single-mutation variants at position 258, we further validated that the L289 reposition is attributed to the combined effect of quadruple mutations. In order to further explore the substrate scope of PAMO we also studied the binding of cyclopentanone and 2-phenylcyclohexanone, which are the typical substrates of CPMO in group I and CHMO in group III, respectively. Our study provides fundamental atomic-level insights in rational engineering of PAMO for wide applications in industrial biocatalysis, in particular, in the biotransformation of long-chain aliphatic oils into potential biodiesels.
[Mh] Termos MeSH primário: Oxigenases de Função Mista/metabolismo
[Mh] Termos MeSH secundário: Acetona/análogos & derivados
Acetona/química
Acetona/metabolismo
Actinobacteria/enzimologia
Sequência de Aminoácidos
Sítios de Ligação
Biocatálise
Domínio Catalítico
Cetonas/química
Cetonas/metabolismo
Cinética
Oxigenases de Função Mista/química
Oxigenases de Função Mista/genética
Simulação de Dinâmica Molecular
Mutagênese Sítio-Dirigida
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/química
Proteínas Recombinantes/isolamento & purificação
Alinhamento de Sequência
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ketones); 0 (Recombinant Proteins); 1364PS73AF (Acetone); EC 1.- (Mixed Function Oxygenases); J2G84H29AF (2-octanone); O7IZH10V9Y (1-phenyl-2-propanone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp07172h


  2 / 16729 MEDLINE  
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[PMID]:28450733
[Au] Autor:An J; Rao A; Ko M
[Ad] Endereço:Department of Biological Sciences, Chonbuk National University, Jeonju, Korea.
[Ti] Título:TET family dioxygenases and DNA demethylation in stem cells and cancers.
[So] Source:Exp Mol Med;49(4):e323, 2017 04 28.
[Is] ISSN:2092-6413
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The methylation of cytosine and subsequent oxidation constitutes a fundamental epigenetic modification in mammalian genomes, and its abnormalities are intimately coupled to various pathogenic processes including cancer development. Enzymes of the Ten-eleven translocation (TET) family catalyze the stepwise oxidation of 5-methylcytosine in DNA to 5-hydroxymethylcytosine and further oxidation products. These oxidized 5-methylcytosine derivatives represent intermediates in the reversal of cytosine methylation, and also serve as stable epigenetic modifications that exert distinctive regulatory roles. It is becoming increasingly obvious that TET proteins and their catalytic products are key regulators of embryonic development, stem cell functions and lineage specification. Over the past several years, the function of TET proteins as a barrier between normal and malignant states has been extensively investigated. Dysregulation of TET protein expression or function is commonly observed in a wide range of cancers. Notably, TET loss-of-function is causally related to the onset and progression of hematologic malignancy in vivo. In this review, we focus on recent advances in the mechanistic understanding of DNA methylation-demethylation dynamics, and their potential regulatory functions in cellular differentiation and oncogenic transformation.
[Mh] Termos MeSH primário: Metilação de DNA
Oxigenases de Função Mista/metabolismo
Neoplasias/genética
Proteínas Proto-Oncogênicas/metabolismo
Células-Tronco/metabolismo
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Epigênese Genética
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Oxigenases de Função Mista/genética
Neoplasias/enzimologia
Neoplasias/metabolismo
Proteínas Proto-Oncogênicas/genética
Células-Tronco/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Proto-Oncogene Proteins); EC 1.- (Mixed Function Oxygenases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180224
[Lr] Data última revisão:
180224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1038/emm.2017.5


  3 / 16729 MEDLINE  
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[PMID]:28467360
[Au] Autor:Herzfeld E; Speh L; Strauss C; Scheller C
[Ad] Endereço:Department of Neurosurgery, Martin-Luther University of Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany. eva.herzfeld@uk-halle.de.
[Ti] Título:Nimodipine but Not Nifedipine Promotes Expression of Fatty Acid 2-Hydroxylase in a Surgical Stress Model Based on Neuro2a Cells.
[So] Source:Int J Mol Sci;18(5), 2017 May 03.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Nimodipine is well characterized for the management of aneurysmal subarachnoid hemorrhage and has been shown to promote a better outcome and less delayed ischemic neurological deficits. Animal and clinical trials show neuroprotective efficacy following nerve injuries. We showed a neuroprotective effect on Neuro2a cells. Subsequent microarray analysis revealed-among others-fatty acid 2-hydroxylase (FA2H) upregulated by nimodipine in vitro, which is a component of myelin synthesis. Differentiated Neuro2a cells were analyzed for nimodipine-mediated survival considering stress treatment in comparison to nifedipine-treatment. Cell survival was determined by measurement of LDH activity in the culture medium. Nimodipine decreased surgery-like stress-induced cell death of differentiated Neuro2a cells. Neuro2a cell culture was analyzed for changes in FA2H expression induced by nimodipine or nifedipine in surgery-like stress conditions. We analyzed expression levels of FA2H mRNA and protein by qPCR using specific primers or a FA2H-specific antibody in nimodipine or nifedipine non- and pre-treated Neuro2a cell culture, respectively. Nimodipine but not nifedipine increases FA2H protein levels and also significantly increases mRNA levels of FA2H in both undifferentiated and differentiated Neuro2a cells. Our findings indicate that higher expression of FA2H induced by nimodipine may cause higher survival of Neuro2a cells stressed with surgery-like stressors.
[Mh] Termos MeSH primário: Bloqueadores dos Canais de Cálcio/farmacologia
Resposta ao Choque Térmico/efeitos dos fármacos
Oxigenases de Função Mista/metabolismo
Fármacos Neuroprotetores/farmacologia
Procedimentos Neurocirúrgicos/efeitos adversos
Nifedipino/farmacologia
Nimodipino/farmacologia
Estresse Oxidativo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Morte Celular/efeitos dos fármacos
Diferenciação Celular/efeitos dos fármacos
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Expressão Gênica
Camundongos
Oxigenases de Função Mista/genética
Bainha de Mielina/metabolismo
Nimodipino/uso terapêutico
RNA Mensageiro/genética
Estresse Mecânico
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium Channel Blockers); 0 (Neuroprotective Agents); 0 (RNA, Messenger); 57WA9QZ5WH (Nimodipine); EC 1.- (Mixed Function Oxygenases); EC 1.- (fatty acid alpha-hydroxylase); I9ZF7L6G2L (Nifedipine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  4 / 16729 MEDLINE  
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[PMID]:29337257
[Au] Autor:Dimopoulou M; Verhoef A; Gomes CA; van Dongen CW; Rietjens IMCM; Piersma AH; van Ravenzwaay B
[Ad] Endereço:Division of Toxicology, Wageningen University, The Netherlands; National Institute of Public Health and the Environment (RIVM), Bilthoven, The Netherlands. Electronic address: myrto.dimopoulou@wur.nl.
[Ti] Título:A comparison of the embryonic stem cell test and whole embryo culture assay combined with the BeWo placental passage model for predicting the embryotoxicity of azoles.
[So] Source:Toxicol Lett;286:10-21, 2018 Apr.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In the present study, we show the value of combining toxico-dynamic and -kinetic in vitro approaches for embryotoxicity testing of azoles. Both the whole embryo culture (WEC) and the embryonic stem cells test (EST) predicted the in vivo potency ranking of twelve tested azoles with moderate accuracy. Combining these results with relative placental transfer rates (Papp values) as determined in the BeWo cell culture model, increased the predictability of both WEC and EST, with R values increasing from 0.51 to 0.87 and from 0.35 to 0.60, respectively. The comparison of these in vitro systems correlated well (R = 0.67), correctly identifying the in vivo strong and weak embryotoxicants. Evaluating also specific gene responses related with the retinoic acid and sterol biosynthesis pathways, which represent the toxicological and fungicidal mode of action of azoles respectively in the WEC and EST, we observed that the differential regulation of Dhrs3 and Msmo1 reached higher magnitudes in both systems compared to Cyp26a1 and Cyp51. Establishing sensitive biomarkers across the in vitro systems for studying the underlying mechanism of action of chemicals, such as azoles, is valuable for comparing alternative in vitro models and for improving insight in the mechanism of developmental toxicity of chemicals.
[Mh] Termos MeSH primário: Azóis/toxicidade
Bioensaio
Embrião de Mamíferos/efeitos dos fármacos
Células-Tronco Embrionárias Murinas/efeitos dos fármacos
Placenta/efeitos dos fármacos
Teratogênios/toxicidade
Testes de Toxicidade/métodos
[Mh] Termos MeSH secundário: Oxirredutases do Álcool/genética
Oxirredutases do Álcool/metabolismo
Animais
Transporte Biológico
Diferenciação Celular/efeitos dos fármacos
Linhagem Celular
Relação Dose-Resposta a Droga
Técnicas de Cultura Embrionária
Embrião de Mamíferos/metabolismo
Embrião de Mamíferos/patologia
Feminino
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Seres Humanos
Cinética
Camundongos
Oxigenases de Função Mista/genética
Oxigenases de Função Mista/metabolismo
Células-Tronco Embrionárias Murinas/metabolismo
Células-Tronco Embrionárias Murinas/patologia
Miócitos Cardíacos/efeitos dos fármacos
Miócitos Cardíacos/metabolismo
Miócitos Cardíacos/patologia
Placenta/metabolismo
Placenta/patologia
Gravidez
Reprodutibilidade dos Testes
Ácido Retinoico 4 Hidroxilase/genética
Ácido Retinoico 4 Hidroxilase/metabolismo
Medição de Risco
Esterol 14-Desmetilase/genética
Esterol 14-Desmetilase/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azoles); 0 (Teratogens); EC 1.- (Mixed Function Oxygenases); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.1.71 (DHRS3 protein, mouse); EC 1.14.13.70 (Cyp51 protein, mouse); EC 1.14.13.70 (Sterol 14-Demethylase); EC 1.14.13.72 (methylsterol monooxygenase); EC 1.14.14.1 (Cyp26a1 protein, mouse); EC 1.14.14.1 (Retinoic Acid 4-Hydroxylase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE


  5 / 16729 MEDLINE  
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[PMID]:28467664
[Au] Autor:Gamella M; Privman M; Bakshi S; Melman A; Katz E
[Ad] Endereço:Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, 13699-5810, USA.
[Ti] Título:DNA Release from Fe -Cross-Linked Alginate Films Triggered by Logically Processed Biomolecular Signals: Integration of Biomolecular Computing and Actuation.
[So] Source:Chemphyschem;18(13):1811-1821, 2017 Jul 05.
[Is] ISSN:1439-7641
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Signal-controlled release of DNA from Fe -cross-linked alginate hydrogel electrochemically deposited on an electrode surface was studied. The multiple input signals were logically processed with the help of the enzyme-biocatalyzed reactions. Boolean logic gates, OR, AND, INH, were realized with the biocatalytic reactions performed by the enzymes entrapped in the alginate film. Hydrogen peroxide produced by the enzymatic reactions resulted in the degradation of the alginate hydrogel and DNA release. The alginate degradation was facilitated by the formation of free radicals in the Fenton-type reaction catalyzed by iron cations cross-linking the alginate hydrogel. The studied approach is versatile and can be adapted to various chemical signals processed by various enzymes with differently implemented Boolean logic. This work illustrates a novel concept of functional integration of biomolecular computing and actuation.
[Mh] Termos MeSH primário: Alginatos/química
Computadores Moleculares
Reagentes para Ligações Cruzadas/química
DNA/metabolismo
Compostos Férricos/química
Lógica
[Mh] Termos MeSH secundário: Animais
Biocatálise
DNA/química
Esterases/química
Esterases/metabolismo
Glucose Oxidase/química
Glucose Oxidase/metabolismo
Ácido Glucurônico/química
Ácidos Hexurônicos/química
Peroxidase do Rábano Silvestre/química
Peroxidase do Rábano Silvestre/metabolismo
Lactato Desidrogenases/química
Lactato Desidrogenases/metabolismo
Oxigenases de Função Mista/química
Oxigenases de Função Mista/metabolismo
Nanopartículas/química
Nanopartículas/metabolismo
Dióxido de Silício/química
Dióxido de Silício/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Cross-Linking Reagents); 0 (Ferric Compounds); 0 (Hexuronic Acids); 7631-86-9 (Silicon Dioxide); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); 9007-49-2 (DNA); EC 1.- (Mixed Function Oxygenases); EC 1.1.- (Lactate Dehydrogenases); EC 1.1.3.4 (Glucose Oxidase); EC 1.11.1.- (Horseradish Peroxidase); EC 1.13.12.4 (lactate 2-monooxygenase); EC 3.1.- (Esterases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1002/cphc.201700301


  6 / 16729 MEDLINE  
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[PMID]:29326044
[Au] Autor:Nigam R; Anindya R
[Ad] Endereço:Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, Hyderabad 502285, Telangana, India.
[Ti] Título:Escherichia coli single-stranded DNA binding protein SSB promotes AlkB-mediated DNA dealkylation repair.
[So] Source:Biochem Biophys Res Commun;496(2):274-279, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Repair of alkylation damage in DNA is essential for maintaining genome integrity. Escherichia coli (E.coli) protein AlkB removes various alkyl DNA adducts including N1-methyladenine (N meA) and N3-methylcytosine (N meC) by oxidative demethylation. Previous studies showed that AlkB preferentially removes N meA and N meC from single-stranded DNA (ssDNA). It can also remove N meA and N meC from double-stranded DNA by base-flipping. Notably, ssDNA produced during DNA replication and recombination, remains bound to E. coli single-stranded DNA binding protein SSB and it is not known whether AlkB can repair methyl adduct present in SSB-coated DNA. Here we have studied AlkB-mediated DNA repair using SSB-bound DNA as substrate. In vitro repair reaction revealed that AlkB could efficiently remove N meC adducts inasmuch as DNA length is shorter than 20 nucleotides. However, when longer N meC-containing oligonuleotides were used as the substrate, efficiency of AlkB catalyzed reaction was abated compared to SSB-bound DNA substrate of identical length. Truncated SSB containing only the DNA binding domain could also support the stimulation of AlkB activity, suggesting the importance of SSB-DNA interaction for AlkB function. Using 70-mer oligonucleotide containing single N meC we demonstrate that SSB-AlkB interaction promotes faster repair of the methyl DNA adducts.
[Mh] Termos MeSH primário: Reparo do DNA
DNA Bacteriano/genética
Proteínas de Ligação a DNA/genética
Proteínas de Escherichia coli/genética
Escherichia coli/genética
Regulação Bacteriana da Expressão Gênica
Oxigenases de Função Mista/genética
[Mh] Termos MeSH secundário: Alquilação
DNA/genética
DNA/metabolismo
Adutos de DNA/química
Adutos de DNA/metabolismo
Dano ao DNA
Metilação de DNA
DNA Bacteriano/metabolismo
DNA de Cadeia Simples/genética
DNA de Cadeia Simples/metabolismo
Proteínas de Ligação a DNA/metabolismo
Escherichia coli/metabolismo
Proteínas de Escherichia coli/metabolismo
Cinética
Oxigenases de Função Mista/metabolismo
Oligonucleotídeos/química
Oligonucleotídeos/metabolismo
Oxirredução
Ligação Proteica
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Adducts); 0 (DNA, Bacterial); 0 (DNA, Single-Stranded); 0 (DNA-Binding Proteins); 0 (Escherichia coli Proteins); 0 (Oligonucleotides); 0 (SSB protein, E coli); 9007-49-2 (DNA); EC 1.- (Mixed Function Oxygenases); EC 1.14.11.- (AlkB protein, E coli)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


  7 / 16729 MEDLINE  
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[PMID]:29337064
[Au] Autor:Knollenberg BJ; Liu J; Yu S; Lin H; Tian L
[Ad] Endereço:Department of Plant Sciences, University of California, Davis, CA, 95616, USA; The Huck Institutes of the Life Sciences, Pennsylvania State University, State College, PA, 16801, USA.
[Ti] Título:Cloning and functional characterization of a p-coumaroyl quinate/shikimate 3'-hydroxylase from potato (Solanum tuberosum).
[So] Source:Biochem Biophys Res Commun;496(2):462-467, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chlorogenic acid (CGA) plays an important role in protecting plants against pathogens and promoting human health. Although CGA accumulates to high levels in potato tubers, the key enzyme p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) for CGA biosynthesis has not been isolated and functionally characterized in potato. In this work, we cloned StC3'H from potato and showed that it catalyzed the formation of caffeoylshikimate and CGA (caffeoylquinate) from p-coumaroyl shikimate and p-coumaroyl quinate, respectively, but was inactive towards p-coumaric acid in in vitro enzyme assays. When the expression of StC3'H proteins was blocked through antisense (AS) inhibition under the control of a tuber-specific patatin promoter, moderate changes in tuber yield as well as phenolic metabolites in the core tuber tissue were observed for several AS lines. On the other hand, the AS and control potato lines exhibited similar responses to a bacterial pathogen Pectobacterium carotovorum. These results suggest that StC3'H is implicated in phenolic metabolism in potato. They also suggest that CGA accumulation in the core tissue of potato tubers is an intricately controlled process and that additional C3'H activity may also be involved in CGA biosynthesis in potato.
[Mh] Termos MeSH primário: Ácido Clorogênico/metabolismo
Oxigenases de Função Mista/genética
Proteínas de Plantas/genética
Tubérculos/enzimologia
Solanum tuberosum/enzimologia
[Mh] Termos MeSH secundário: Hidrolases de Éster Carboxílico/genética
Hidrolases de Éster Carboxílico/metabolismo
Ácido Clorogênico/análogos & derivados
Clonagem Molecular
Expressão Gênica
Oxigenases de Função Mista/antagonistas & inibidores
Oxigenases de Função Mista/metabolismo
Oligonucleotídeos Antissenso/genética
Oligonucleotídeos Antissenso/metabolismo
Pectobacterium carotovorum/patogenicidade
Pectobacterium carotovorum/fisiologia
Pichia/genética
Pichia/metabolismo
Proteínas de Plantas/metabolismo
Tubérculos/genética
Tubérculos/microbiologia
Plantas Geneticamente Modificadas
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Ácido Chiquímico/análogos & derivados
Ácido Chiquímico/metabolismo
Solanum tuberosum/genética
Solanum tuberosum/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (4-coumaroylshikimic acid); 0 (Oligonucleotides, Antisense); 0 (Plant Proteins); 0 (Recombinant Proteins); 0 (patatin protein, Solanum tuberosum); 29MS2WI2NU (Shikimic Acid); 318ADP12RI (Chlorogenic Acid); 73263-62-4 (5-O-caffeoylshikimic acid); E57A0DKE0B (3,4-di-O-caffeoylquinic acid); EC 1.- (Mixed Function Oxygenases); EC 1.14.13.- (5-O-(4-coumaroyl)shikimate 3'-hydroxylase); EC 3.1.1.- (Carboxylic Ester Hydrolases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE


  8 / 16729 MEDLINE  
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[PMID]:29175454
[Au] Autor:Rea M; Gripshover T; Fondufe-Mittendorf Y
[Ad] Endereço:Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA.
[Ti] Título:Selective inhibition of CTCF binding by iAs directs TET-mediated reprogramming of 5-hydroxymethylation patterns in iAs-transformed cells.
[So] Source:Toxicol Appl Pharmacol;338:124-133, 2018 01 01.
[Is] ISSN:1096-0333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Methylation at cytosine (5mC) is a fundamental epigenetic DNA modification recently associated with iAs-mediated carcinogenesis. In contrast, the role of 5-hydroxymethylcytosine (5hmC), the oxidation product of 5mC in iAs-mediated carcinogenesis is unknown. Here we assess the hydroxymethylome in iAs-transformed cells, showing that dynamic modulation of hydroxymethylated DNA is associated with specific transcriptional networks. Moreover, this pathologic iAs-mediated carcinogenesis is characterized by a shift toward a higher hydroxymethylation pattern genome-wide. At specific promoters, hydroxymethylation correlated with increased gene expression. Furthermore, this increase in hydroxymethylation occurs concurrently with an upregulation of ten-eleven translocation (TET) enzymes that oxidize 5-methylcytosine (5mC) in DNA. To gain an understanding into how iAs might impact TET expression, we found that iAs inhibits the binding of CTCF at the proximal, weak CTCF binding sites of the TET1 and TET2 gene promoters and enhances CTCF binding at the stronger distal binding site. Further analyses suggest that this distal site acts as an enhancer, thus high CTCF occupancy at the enhancer region of TET1 and TET2 possibly drives their high expression in iAs-transformed cells. These results have major implications in understanding the impact of differential CTCF binding, genome architecture and its consequences in iAs-mediated pathogenesis.
[Mh] Termos MeSH primário: Arsênico/toxicidade
Fator de Ligação a CCCTC/antagonistas & inibidores
Proteínas de Ligação a DNA/fisiologia
Oxigenases de Função Mista/fisiologia
Proteínas Proto-Oncogênicas/fisiologia
[Mh] Termos MeSH secundário: 5-Metilcitosina/análogos & derivados
5-Metilcitosina/análise
Fator de Ligação a CCCTC/metabolismo
Transformação Celular Neoplásica
Metilação de DNA
Proteínas de Ligação a DNA/genética
Seres Humanos
Oxigenases de Função Mista/genética
Regiões Promotoras Genéticas
Proteínas Proto-Oncogênicas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (CCCTC-Binding Factor); 0 (CTCF protein, human); 0 (DNA-Binding Proteins); 0 (Proto-Oncogene Proteins); 0 (TET2 protein, human); 1123-95-1 (5-hydroxymethylcytosine); 6R795CQT4H (5-Methylcytosine); EC 1.- (Mixed Function Oxygenases); EC 1.- (TET1 protein, human); N712M78A8G (Arsenic)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  9 / 16729 MEDLINE  
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[PMID]:28742265
[Au] Autor:Alsubhi S; Alhashem A; Faqeih E; Alfadhel M; Alfaifi A; Altuwaijri W; Alsahli S; Aldhalaan H; Alkuraya FS; Hundallah K; Mahmoud A; Alasmari A; Mutairi FA; Abduraouf H; AlRasheed L; Alshahwan S; Tabarki B
[Ad] Endereço:Division of Pediatric Neurology, Department of Pediatrics, Prince Sultan Military Medical City, Riyadh, Saudi Arabia.
[Ti] Título:Congenital disorders of glycosylation: The Saudi experience.
[So] Source:Am J Med Genet A;173(10):2614-2621, 2017 Oct.
[Is] ISSN:1552-4833
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We retrospectively reviewed Saudi patients who had a congenital disorder of glycosylation (CDG). Twenty-seven Saudi patients (14 males, 13 females) from 13 unrelated families were identified. Based on molecular studies, the 27 CDG patients were classified into different subtypes: ALG9-CDG (8 patients, 29.5%), ALG3-CDG (7 patients, 26%), COG6-CDG (7 patients, 26%), MGAT2-CDG (3 patients, 11%), SLC35A2-CDG (1 patient), and PMM2-CDG (1 patient). All the patients had homozygous gene mutations. The combined carrier frequency of CDG for the encountered founder mutations in the Saudi population is 11.5 per 10,000, which translates to a minimum disease burden of 14 patients per 1,000,000. Our study provides comprehensive epidemiologic information and prevalence figures for each of these CDG in a large cohort of congenital disorder of glycosylation patients.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/genética
Defeitos Congênitos da Glicosilação/genética
Mutação
[Mh] Termos MeSH secundário: Proteínas Adaptadoras de Transporte Vesicular/genética
Adolescente
Criança
Pré-Escolar
Defeitos Congênitos da Glicosilação/epidemiologia
Feminino
Glicosilação
Homozigoto
Seres Humanos
Lactente
Masculino
Manosiltransferases/genética
Proteínas de Membrana/genética
Oxigenases de Função Mista/genética
Proteínas de Transporte de Monossacarídeos/genética
N-Acetilglucosaminiltransferases/genética
Fenótipo
Estudos Retrospectivos
Arábia Saudita/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Proteins, Vesicular Transport); 0 (Biomarkers, Tumor); 0 (COG6 protein, human); 0 (Membrane Proteins); 0 (Monosaccharide Transport Proteins); 0 (UDP-galactose translocator); EC 1.- (Mixed Function Oxygenases); EC 1.14.17.- (4-coumaroyl-D-glucose hydroxylase); EC 2.4.1.- (ALG3 protein, human); EC 2.4.1.- (ALG9 protein, human); EC 2.4.1.- (Mannosyltransferases); EC 2.4.1.- (N-Acetylglucosaminyltransferases); EC 2.4.1.143 (alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1002/ajmg.a.38358


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[PMID]:28745640
[Au] Autor:Cao C; Wang WJ; Huang YY; Yao HL; Conway LP; Liu L; Voglmeir J
[Ad] Endereço:Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University.
[Ti] Título:Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.
[So] Source:J Vis Exp;(125), 2017 Jul 14.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:CMAH (cytidine monophosphate-N-acetylneuraminic acid hydroxylase) is responsible for the oxidation of cytidine monophosphate-N-acetylneuraminic acids in mammals. However, humans cannot oxidize cytidine monophosphate-N-acetylneuraminic acid to cytidine monophosphate-N-glycolylneuraminic acid due to a primary exon deletion of the CMAH gene. To understand the effects and implications of the lack of CMAH activity in more detail, a Cmah knock-out model in mice is of keen interest in basic and applied research. The analysis method to determine the phenotype of this mouse model is herein described in detail, and is based on the detection of both N-acetylneuraminic acid and N-glycolylenuraminic acid in the liver and milk of wild-type and Cmah knock-out mice. Endogenous sialic acids are released and derivatized with o-phenylenediamine to generate fluorogenic derivatives, which can be subsequently analyzed by HPLC. The presented protocol can be also applied for the analysis of milk and tissue samples from various other origins, and may be of use to investigate the nutritional and health effects of N-glycolylneuraminic acid.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão
Fígado/química
Leite/química
Oxigenases de Função Mista/genética
Ácidos Siálicos/análise
[Mh] Termos MeSH secundário: Animais
Sistemas CRISPR-Cas/genética
Fígado/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Oxigenases de Função Mista/deficiência
Ácidos Siálicos/isolamento & purificação
Gravação em Vídeo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sialic Acids); EC 1.- (Mixed Function Oxygenases); EC 1.14.18.2 (CMPacetylneuraminate monooxygenase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.3791/56030



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