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Pesquisa : D08.811.739.800 [Categoria DeCS]
Referências encontradas : 86 [refinar]
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[PMID]:26911466
[Au] Autor:Ike Y
[Ti] Título:[Pathogenicity of Enterococcus].
[So] Source:Nihon Saikingaku Zasshi;71(1):1-2, 2016.
[Is] ISSN:1882-4110
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Mh] Termos MeSH primário: Enterococcus/genética
Enterococcus/patogenicidade
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Bacteriocinas
Elementos de DNA Transponíveis
Farmacorresistência Bacteriana
Enterococcus/efeitos dos fármacos
Evolução Molecular
Seres Humanos
Perforina
Transposon Resolvases
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; LECTURES; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacteriocins); 0 (DNA Transposable Elements); 126465-35-8 (Perforin); EC 2.7.7.- (Transposon Resolvases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170106
[Lr] Data última revisão:
170106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160226
[St] Status:MEDLINE
[do] DOI:10.3412/jsb.71.1


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[PMID]:25990737
[Au] Autor:Olorunniji FJ; McPherson AL; Pavlou HJ; McIlwraith MJ; Brazier JA; Cosstick R; Stark WM
[Ad] Endereço:Institute of Molecular, Cell and Systems Biology, University of Glasgow, Bower Building, Glasgow G12 8QQ, UK.
[Ti] Título:Nicked-site substrates for a serine recombinase reveal enzyme-DNA communications and an essential tethering role of covalent enzyme-DNA linkages.
[So] Source:Nucleic Acids Res;43(12):6134-43, 2015 Jul 13.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To analyse the mechanism and kinetics of DNA strand cleavages catalysed by the serine recombinase Tn3 resolvase, we made modified recombination sites with a single-strand nick in one of the two DNA strands. Resolvase acting on these sites cleaves the intact strand very rapidly, giving an abnormal half-site product which accumulates. We propose that these reactions mimic second-strand cleavage of an unmodified site. Cleavage occurs in a synapse of two sites, held together by a resolvase tetramer; cleavage at one site stimulates cleavage at the partner site. After cleavage of a nicked-site substrate, the half-site that is not covalently linked to a resolvase subunit dissociates rapidly from the synapse, destabilizing the entire complex. The covalent resolvase-DNA linkages in the natural reaction intermediate thus perform an essential DNA-tethering function. Chemical modifications of a nicked-site substrate at the positions of the scissile phosphodiesters result in abolition or inhibition of resolvase-mediated cleavage and effects on resolvase binding and synapsis, providing insight into the serine recombinase catalytic mechanism and how resolvase interacts with the substrate DNA.
[Mh] Termos MeSH primário: Clivagem do DNA
DNA/metabolismo
Transposon Resolvases/metabolismo
[Mh] Termos MeSH secundário: DNA/química
Cinética
Recombinação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
9007-49-2 (DNA); EC 2.7.7.- (Tn3 resolvase); EC 2.7.7.- (Transposon Resolvases)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150521
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkv521


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[PMID]:24647540
[Au] Autor:Norberg P; Bergström M; Hermansson M
[Ad] Endereço:Department of Infectious Diseases, University of Gothenburg, Göteborg, Sweden.
[Ti] Título:Complete nucleotide sequence and analysis of two conjugative broad host range plasmids from a marine microbial biofilm.
[So] Source:PLoS One;9(3):e92321, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The complete nucleotide sequence of plasmids pMCBF1 and pMCBF6 was determined and analyzed. pMCBF1 and pMCBF6 form a novel clade within the IncP-1 plasmid family designated IncP-1 ς. The plasmids were exogenously isolated earlier from a marine biofilm. pMCBF1 (62 689 base pairs; bp) and pMCBF6 (66 729 bp) have identical backbones, but differ in their mercury resistance transposons. pMCBF1 carries Tn5053 and pMCBF6 carries Tn5058. Both are flanked by 5 bp direct repeats, typical of replicative transposition. Both insertions are in the vicinity of a resolvase gene in the backbone, supporting the idea that both transposons are "res-site hunters" that preferably insert close to and use external resolvase functions. The similarity of the backbones indicates recent insertion of the two transposons and the ongoing dynamics of plasmid evolution in marine biofilms. Both plasmids also carry the insertion sequence ISPst1, albeit without flanking repeats. ISPs1is located in an unusual site within the control region of the plasmid. In contrast to most known IncP-1 plasmids the pMCBF1/pMCBF6 backbone has no insert between the replication initiation gene (trfA) and the vegetative replication origin (oriV). One pMCBF1/pMCBF6 block of about 2.5 kilo bases (kb) has no similarity with known sequences in the databases. Furthermore, insertion of three genes with similarity to the multidrug efflux pump operon mexEF and a gene from the NodT family of the tripartite multi-drug resistance-nodulation-division (RND) system in Pseudomonas aeruginosa was found. They do not seem to confer antibiotic resistance to the hosts of pMCBF1/pMCBF6, but the presence of RND on promiscuous plasmids may have serious implications for the spread of antibiotic multi-resistance.
[Mh] Termos MeSH primário: Sequência de Bases/genética
Biofilmes/crescimento & desenvolvimento
Plasmídeos/genética
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Filogenia
Transposon Resolvases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 2.7.7.- (Transposon Resolvases)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140321
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0092321


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[PMID]:24286749
[Au] Autor:Ma CH; Liu YT; Savva CG; Rowley PA; Cannon B; Fan HF; Russell R; Holzenburg A; Jayaram M
[Ad] Endereço:Section of Molecular Genetics and Microbiology, Institute of Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.
[Ti] Título:Organization of DNA partners and strand exchange mechanisms during Flp site-specific recombination analyzed by difference topology, single molecule FRET and single molecule TPM.
[So] Source:J Mol Biol;426(4):793-815, 2014 Feb 20.
[Is] ISSN:1089-8638
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Flp site-specific recombination between two target sites (FRTs) harboring non-homology within the strand exchange region does not yield stable recombinant products. In negatively supercoiled plasmids containing head-to-tail sites, the reaction produces a series of knots with odd-numbered crossings. When the sites are in head-to-head orientation, the knot products contain even-numbered crossings. Both types of knots retain parental DNA configuration. By carrying out Flp recombination after first assembling the topologically well defined Tn3 resolvase synapse, it is possible to determine whether these knots arise by a processive or a dissociative mechanism. The nearly exclusive products from head-to-head and head-to-tail oriented "non-homologous" FRT partners are a 4-noded knot and a 5-noded knot, respectively. The corresponding products from a pair of native (homologous) FRT sites are a 3-noded knot and a 4-noded catenane, respectively. These results are consistent with non-homology-induced two rounds of dissociative recombination by Flp, the first to generate reciprocal recombinants containing non-complementary base pairs and the second to produce parental molecules with restored base pairing. Single molecule fluorescence resonance energy transfer (smFRET) analysis of geometrically restricted FRTs, together with single molecule tethered particle motion (smTPM) assays of unconstrained FRTs, suggests that the sites are preferentially synapsed in an anti-parallel fashion. This selectivity in synapse geometry occurs prior to the chemical steps of recombination, signifying early commitment to a productive reaction path. The cumulative topological, smFRET and smTPM results have implications for the relative orientation of DNA partners and the directionality of strand exchange during recombination mediated by tyrosine site-specific recombinases.
[Mh] Termos MeSH primário: DNA Nucleotidiltransferases/metabolismo
DNA/química
DNA/genética
Recombinação Genética
[Mh] Termos MeSH secundário: DNA Nucleotidiltransferases/genética
DNA Super-Helicoidal/genética
Transferência Ressonante de Energia de Fluorescência/métodos
Biologia Molecular/métodos
Conformação de Ácido Nucleico
Transposon Resolvases/genética
Transposon Resolvases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Superhelical); 9007-49-2 (DNA); EC 2.7.7.- (DNA Nucleotidyltransferases); EC 2.7.7.- (FLP recombinase); EC 2.7.7.- (Tn3 resolvase); EC 2.7.7.- (Transposon Resolvases)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:140128
[Lr] Data última revisão:
140128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131130
[St] Status:MEDLINE


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[PMID]:22733069
[Au] Autor:Søndergaard A; San Millan A; Santos-Lopez A; Nielsen SM; Gonzalez-Zorn B; Nørskov-Lauritsen N
[Ad] Endereço:Department of Clinical Microbiology, Aarhus University Hospital, Aarhus, Denmark.
[Ti] Título:Molecular organization of small plasmids bearing blaTEM-1 and conferring resistance to ß-lactams in Haemophilus influenzae.
[So] Source:Antimicrob Agents Chemother;56(9):4958-60, 2012 Sep.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:TEM-1 is the dominant ß-lactamase of Haemophilus influenzae and can be located on small plasmids. Three distinct plasmids with sizes from 4,304 to 5,646 nucleotides (nt) were characterized: pA1606, pA1209, and pPN223. In addition to TEM-1 and a replication enzyme of the Rep 3 superfamily, pA1606 carries a Tn3 resolvase gene and pA1606 and pA1209 carry an open reading frame (ORF) similar to a plasmid recombination enzyme gene described in Gram-positive bacteria. The plasmids transformed strain Rd to the ampicillin-resistant phenotype.
[Mh] Termos MeSH primário: Haemophilus influenzae/genética
Plasmídeos
Resistência beta-Lactâmica/genética
beta-Lactamases/genética
[Mh] Termos MeSH secundário: Ampicilina/farmacologia
Sequência de Bases
Haemophilus influenzae/isolamento & purificação
Testes de Sensibilidade Microbiana
Dados de Sequência Molecular
Fases de Leitura Aberta
RNA Replicase/genética
Recombinases/genética
Transformação Bacteriana
Transposon Resolvases/genética
beta-Lactamas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinases); 0 (beta-Lactams); 7C782967RD (Ampicillin); EC 2.7.7.- (Tn3 resolvase); EC 2.7.7.- (Transposon Resolvases); EC 2.7.7.48 (RNA Replicase); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase TEM-1)
[Em] Mês de entrada:1301
[Cu] Atualização por classe:150224
[Lr] Data última revisão:
150224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120627
[St] Status:MEDLINE
[do] DOI:10.1128/AAC.00408-12


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[PMID]:21723338
[Au] Autor:Lozano MJ; Salas ME; Giusti MA; Draghi WO; Torres Tejerizo GA; Martini MC; Del Papa MF; Pistorio M; Lagares A
[Ad] Endereço:IBBM - Instituto de Biotecnología y Biología Molecular, CONICET - Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115, 1900 La Plata, Argentina.
[Ti] Título:Development of new positive-selection RIVET tools: detection of induced promoters by the excision-based transcriptional activation of an aacCI (GmR)-gfp fusion.
[So] Source:J Biotechnol;155(2):147-55, 2011 Sep 10.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:RIVET (Recombination Based in vivo Expression Technology) is a powerful genetic tool originally conceived for the identification of genes induced in complex biological niches where conventional transcriptomics is difficult to use. With a broader application, genetic recombination-based technologies have also been used, in combination with regulatory proteins and specific transcriptional regulators, for the development of highly sensitive biosensor systems. RIVET systems generally comprise two modules: a promoter-trap cassette generating genomic transcriptional fusions to the tnpR gene encoding the Tn-γδ TnpR resolvase, and a reporter cassette carrying res-flanked selection markers that are excised upon expression of tnpR to produce an irreversible, inheritable phenotypic change. We report here the construction and validation of a new set of positive-selection RIVET systems that, upon induction of the promoter-trap module, generate the transcriptional activation of an antibiotic-resistant and a green-fluorescent phenotype. Two classes of promoter-trap tools were constructed to generate transcriptional fusions to tnpR: one based on the use of a narrow-host-range plasmid (pRIVET-I), integrative in several Gram-negative bacteria, and the other based on the use of a broad-host-range plasmid (pRIVET-R). The system was evaluated in the model soil bacterium Sinorhizobium meliloti, where a clear-cut phenotypic transition from Nm(R)-Gm(S)-GFP(-) to Nm(S)-Gm(R)-GFP(+) occurred upon expression of tnpR. A S. meliloti integrative RIVET library was constructed in pRIVET-I and, as expected, changes in the extracellular conditions (e.g., salt stress) triggered a significant increase in the appearance of Gm(R)-GFP(+) (excised) clones. The sacB-independent positive-selection RIVET systems here described provide suitable basic tools both for the construction of new recombination-based biosensors and for the search of bacterial markers induced when microorganisms colonize and invade complex environments and eukaryotic hosts.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Perfilação da Expressão Gênica/métodos
Regulação Bacteriana da Expressão Gênica/genética
Proteínas Recombinantes de Fusão/metabolismo
Recombinação Genética/genética
Sinorhizobium meliloti/metabolismo
Ativação Transcricional/genética
[Mh] Termos MeSH secundário: Farmacorresistência Bacteriana/genética
Escherichia coli
Biblioteca Gênica
Proteínas de Fluorescência Verde
Plasmídeos/genética
Regiões Promotoras Genéticas/genética
Sinorhizobium meliloti/genética
Transposon Resolvases/metabolismo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Recombinant Fusion Proteins); 147336-22-9 (Green Fluorescent Proteins); EC 2.7.7.- (Transposon Resolvases)
[Em] Mês de entrada:1112
[Cu] Atualização por classe:110815
[Lr] Data última revisão:
110815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110705
[St] Status:MEDLINE
[do] DOI:10.1016/j.jbiotec.2011.06.014


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[PMID]:21234748
[Au] Autor:Murata M; Uchida T; Yang Y; Lezhava A; Kinashi H
[Ad] Endereço:Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
[Ti] Título:A large inversion in the linear chromosome of Streptomyces griseus caused by replicative transposition of a new Tn3 family transposon.
[So] Source:Arch Microbiol;193(4):299-306, 2011 Apr.
[Is] ISSN:1432-072X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We have comprehensively analyzed the linear chromosomes of Streptomyces griseus mutants constructed and kept in our laboratory. During this study, macrorestriction analysis of AseI and DraI fragments of mutant 402-2 suggested a large chromosomal inversion. The junctions of chromosomal inversion were cloned and sequenced and compared with the corresponding target sequences in the parent strain 2247. Consequently, a transposon-involved mechanism was revealed. Namely, a transposon originally located at the left target site was replicatively transposed to the right target site in an inverted direction, which generated a second copy and at the same time caused a 2.5-Mb chromosomal inversion. The involved transposon named TnSGR was grouped into a new subfamily of the resolvase-encoding Tn3 family transposons based on its gene organization. At the end, terminal diversity of S. griseus chromosomes is discussed by comparing the sequences of strains 2247 and IFO13350.
[Mh] Termos MeSH primário: Inversão Cromossômica
Cromossomos Bacterianos
Retroelementos
Streptomyces griseus/genética
Transposon Resolvases/genética
[Mh] Termos MeSH secundário: Sequência de Bases
DNA Bacteriano/genética
Dados de Sequência Molecular
Mutação
Mapeamento por Restrição
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Retroelements); EC 2.7.7.- (Tn3 resolvase); EC 2.7.7.- (Transposon Resolvases)
[Em] Mês de entrada:1105
[Cu] Atualização por classe:110315
[Lr] Data última revisão:
110315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110115
[St] Status:MEDLINE
[do] DOI:10.1007/s00203-010-0674-5


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[PMID]:21187418
[Au] Autor:Gaj T; Mercer AC; Gersbach CA; Gordley RM; Barbas CF
[Ad] Endereço:Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
[Ti] Título:Structure-guided reprogramming of serine recombinase DNA sequence specificity.
[So] Source:Proc Natl Acad Sci U S A;108(2):498-503, 2011 Jan 11.
[Is] ISSN:1091-6490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Routine manipulation of cellular genomes is contingent upon the development of proteins and enzymes with programmable DNA sequence specificity. Here we describe the structure-guided reprogramming of the DNA sequence specificity of the invertase Gin from bacteriophage Mu and Tn3 resolvase from Escherichia coli. Structure-guided and comparative sequence analyses were used to predict a network of amino acid residues that mediate resolvase and invertase DNA sequence specificity. Using saturation mutagenesis and iterative rounds of positive antibiotic selection, we identified extensively redesigned and highly convergent resolvase and invertase populations in the context of engineered zinc-finger recombinase (ZFR) fusion proteins. Reprogrammed variants selectively catalyzed recombination of nonnative DNA sequences > 10,000-fold more effectively than their parental enzymes. Alanine-scanning mutagenesis revealed the molecular basis of resolvase and invertase DNA sequence specificity. When used as rationally designed ZFR heterodimers, the reprogrammed enzyme variants site-specifically modified unnatural and asymmetric DNA sequences. Early studies on the directed evolution of serine recombinase DNA sequence specificity produced enzymes with relaxed substrate specificity as a result of randomly incorporated mutations. In the current study, we focused our mutagenesis exclusively on DNA determinants, leading to redesigned enzymes that remained highly specific and directed transgene integration into the human genome with > 80% accuracy. These results demonstrate that unique resolvase and invertase derivatives can be developed to site-specifically modify the human genome in the context of zinc-finger recombinase fusion proteins.
[Mh] Termos MeSH primário: DNA Nucleotidiltransferases/genética
Recombinases/genética
Serina/química
Transposon Resolvases/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Bacteriófago mu/metabolismo
Dimerização
Escherichia coli/enzimologia
Marcação de Genes
Genoma Humano
Seres Humanos
Modelos Moleculares
Dados de Sequência Molecular
Mutagênese
Conformação Proteica
Engenharia de Proteínas/métodos
Estrutura Secundária de Proteína
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Recombinases); 452VLY9402 (Serine); EC 2.7.7.- (DNA Nucleotidyltransferases); EC 2.7.7.- (DNA invertase Gin); EC 2.7.7.- (Tn3 resolvase); EC 2.7.7.- (Transposon Resolvases)
[Em] Mês de entrada:1102
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101229
[St] Status:MEDLINE
[do] DOI:10.1073/pnas.1014214108


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[PMID]:20298194
[Au] Autor:Olorunniji FJ; Stark WM
[Ad] Endereço:Faculty of Biomedical and Life Sciences, University of Glasgow, Bower Building, Glasgow G12 8QQ, UK. F.Olorunniji@bio.gla.ac.uk
[Ti] Título:Catalysis of site-specific recombination by Tn3 resolvase.
[So] Source:Biochem Soc Trans;38(2):417-21, 2010 Apr.
[Is] ISSN:1470-8752
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The active-site interactions involved in the catalysis of DNA site-specific recombination by the serine recombinases are still incompletely understood. Recent crystal structures of synaptic gammadelta resolvase-DNA intermediates and biochemical analysis of Tn3 resolvase mutants have provided new insights into the structure of the resolvase active site, and how interactions of the catalytic residues with the DNA substrate might promote the phosphoryl transfer reactions.
[Mh] Termos MeSH primário: Recombinação Genética/fisiologia
Transposon Resolvases/fisiologia
[Mh] Termos MeSH secundário: Catálise
Domínio Catalítico
DNA/metabolismo
Modelos Biológicos
Modelos Moleculares
Recombinases/metabolismo
Recombinases/fisiologia
Serina/metabolismo
Especificidade por Substrato
Transposon Resolvases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Recombinases); 452VLY9402 (Serine); 9007-49-2 (DNA); EC 2.7.7.- (Tn3 resolvase); EC 2.7.7.- (Transposon Resolvases)
[Em] Mês de entrada:1006
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100320
[St] Status:MEDLINE
[do] DOI:10.1042/BST0380417


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[PMID]:20061472
[Au] Autor:Christie-Oleza JA; Nogales B; Lalucat J; Bosch R
[Ad] Endereço:Microbiologia, Departament de Biologia, Universitat de les Illes Balears, Carretera Valldemossa, km 7.5, 07122 Palma de Mallorca, Spain.
[Ti] Título:TnpR encoded by an ISPpu12 isoform regulates transposition of two different ISL3-like insertion sequences in Pseudomonas stutzeri after conjugative interaction.
[So] Source:J Bacteriol;192(5):1423-32, 2010 Mar.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pseudomonas stutzeri AN10 has two ISL3-like insertion sequences (ISs). One of them has been recently described as ISPst9. In this study we show that the second IS, situated 4.5 kb upstream of ISPst9, is an isoform of ISPpu12 from Pseudomonas putida mt-2. Although both ISL3-like ISs are flanked by nearly identical (21/24 conserved residues) inverted repeats (IRs) and harbor similar transposases (93% amino acid identity), they differ in their accompanying genes. As described for ISPst9, the isoform of ISPpu12 also transposes by a conservative mechanism, forms circular double-stranded DNA (dsDNA) transposition intermediates, and is induced by interaction with the conjugative strain Escherichia coli S17-1lambda(pir) (conjugative interaction) but not with the nonconjugative E. coli DH5alpha. In fact, we demonstrate that ISPst9 transposition after conjugative interaction occurs only when ISPpu12 is present, indicating that ISPpu12 is upregulating transposition of both ISs under such conditions. We also demonstrate that this conjugative interaction-mediated induction of ISPpu12 is not exclusive to the P. stutzeri AN10 strain but is a more general phenomenon, at least in Pseudomonas. Mutation of TnpR, a MerR-like transcriptional regulator present in ISPpu12 but not in ISPst9, reduced the transcription of tnpA (ISPpu12 transposase-encoding gene) and decreased formation of circular dsDNA transposition intermediates after conjugative interaction. Complementation of the TnpR mutant restored the phenotype. In addition, the presence of TnpR in an ISPpu12-free genetic background did not induce ISPst9 after conjugative interaction. Thus, our results suggest that TnpR, after conjugative interaction, activates transcription of tnpA of ISPpu12. Then, TnpA of ISPpu12 would bind to IRs of both ISs, ISPpu12 and ISPst9, causing their transposition.
[Mh] Termos MeSH primário: Conjugação Genética
Elementos de DNA Transponíveis
Pseudomonas stutzeri/enzimologia
Pseudomonas stutzeri/genética
Recombinação Genética
Transposon Resolvases/metabolismo
[Mh] Termos MeSH secundário: Sequência de Bases
DNA Bacteriano/química
DNA Bacteriano/genética
Escherichia coli/genética
Deleção de Genes
Teste de Complementação Genética
Dados de Sequência Molecular
Pseudomonas putida/genética
Análise de Sequência de DNA
Homologia de Sequência do Ácido Nucleico
Transposon Resolvases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (DNA, Bacterial); EC 2.7.7.- (Transposon Resolvases)
[Em] Mês de entrada:1003
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100112
[St] Status:MEDLINE
[do] DOI:10.1128/JB.01336-09



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