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  1 / 9767 MEDLINE  
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[PMID]:29311546
[Au] Autor:Yang JE; Park SJ; Kim WJ; Kim HJ; Kim BJ; Lee H; Shin J; Lee SY
[Ad] Endereço:Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Repu
[Ti] Título:One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains.
[So] Source:Nat Commun;9(1):79, 2018 01 08.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aromatic polyesters are widely used plastics currently produced from petroleum. Here we engineer Escherichia coli strains for the production of aromatic polyesters from glucose by one-step fermentation. When the Clostridium difficile isocaprenoyl-CoA:2-hydroxyisocaproate CoA-transferase (HadA) and evolved polyhydroxyalkanoate (PHA) synthase genes are overexpressed in a D-phenyllactate-producing strain, poly(52.3 mol% 3-hydroxybutyrate (3HB)-co-47.7 mol% D-phenyllactate) can be produced from glucose and sodium 3HB. Also, various poly(3HB-co-D-phenyllactate) polymers having 11.0, 15.8, 20.0, 70.8, and 84.5 mol% of D-phenyllactate are produced from glucose as a sole carbon source by additional expression of Ralstonia eutropha ß-ketothiolase (phaA) and reductase (phaB) genes. Fed-batch culture of this engineered strain produces 13.9 g l of poly(61.9 mol% 3HB-co-38.1 mol% D-phenyllactate). Furthermore, different aromatic polyesters containing D-mandelate and D-3-hydroxy-3-phenylpropionate are produced from glucose when feeding the corresponding monomers. The engineered bacterial system will be useful for one-step fermentative production of aromatic polyesters from renewable resources.
[Mh] Termos MeSH primário: Escherichia coli/metabolismo
Fermentação
Glucose/metabolismo
Engenharia Metabólica/métodos
Poliésteres/metabolismo
[Mh] Termos MeSH secundário: Ácido 3-Hidroxibutírico/metabolismo
Acetil-CoA C-Aciltransferase/genética
Acetil-CoA C-Aciltransferase/metabolismo
Aciltransferases/genética
Aciltransferases/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Clostridium difficile/enzimologia
Clostridium difficile/genética
Coenzima A-Transferases/genética
Coenzima A-Transferases/metabolismo
Cupriavidus necator/enzimologia
Cupriavidus necator/genética
Escherichia coli/genética
Lactatos/metabolismo
Oxirredutases/genética
Oxirredutases/metabolismo
Polietilenotereftalatos/metabolismo
Polímeros/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Lactates); 0 (Polyesters); 0 (Polyethylene Terephthalates); 0 (Polymers); 156-05-8 (3-phenyllactic acid); EC 1.- (Oxidoreductases); EC 2.3.- (Acyltransferases); EC 2.3.1.- (poly(3-hydroxyalkanoic acid) synthase); EC 2.3.1.16 (Acetyl-CoA C-Acyltransferase); EC 2.8.3.- (Coenzyme A-Transferases); IY9XDZ35W2 (Glucose); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02498-w


  2 / 9767 MEDLINE  
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[PMID]:29199980
[Au] Autor:Stewart C; Woods K; Macias G; Allan AC; Hellens RP; Noel JP
[Ad] Endereço:Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
[Ti] Título:Molecular architectures of benzoic acid-specific type III polyketide synthases.
[So] Source:Acta Crystallogr D Struct Biol;73(Pt 12):1007-1019, 2017 Dec 01.
[Is] ISSN:2059-7983
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Biphenyl synthase and benzophenone synthase constitute an evolutionarily distinct clade of type III polyketide synthases (PKSs) that use benzoic acid-derived substrates to produce defense metabolites in plants. The use of benzoyl-CoA as an endogenous substrate is unusual for type III PKSs. Moreover, sequence analyses indicate that the residues responsible for the functional diversification of type III PKSs are mutated in benzoic acid-specific type III PKSs. In order to gain a better understanding of structure-function relationships within the type III PKS family, the crystal structures of biphenyl synthase from Malus × domestica and benzophenone synthase from Hypericum androsaemum were compared with the structure of an archetypal type III PKS: chalcone synthase from Malus × domestica. Both biphenyl synthase and benzophenone synthase contain mutations that reshape their active-site cavities to prevent the binding of 4-coumaroyl-CoA and to favor the binding of small hydrophobic substrates. The active-site cavities of biphenyl synthase and benzophenone synthase also contain a novel pocket associated with their chain-elongation and cyclization reactions. Collectively, these results illuminate structural determinants of benzoic acid-specific type III PKSs and expand the understanding of the evolution of specialized metabolic pathways in plants.
[Mh] Termos MeSH primário: Aciltransferases/química
Hypericum/enzimologia
Malus/enzimologia
[Mh] Termos MeSH secundário: Acil Coenzima A/química
Acil Coenzima A/metabolismo
Aciltransferases/metabolismo
Carbono-Carbono Ligases/química
Carbono-Carbono Ligases/metabolismo
Domínio Catalítico
Clonagem Molecular
Cristalografia por Raios X
Evolução Molecular
Modelos Moleculares
Estrutura Molecular
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acyl Coenzyme A); 6756-74-7 (benzoyl-coenzyme A); EC 2.3.- (Acyltransferases); EC 2.3.1.74 (flavanone synthetase); EC 6.4.- (Carbon-Carbon Ligases); EC 6.4.- (benzophenone synthase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.1107/S2059798317016618


  3 / 9767 MEDLINE  
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[PMID]:29326245
[Au] Autor:Rana MS; Kumar P; Lee CJ; Verardi R; Rajashankar KR; Banerjee A
[Ad] Endereço:Cell Biology and Neurobiology Branch, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
[Ti] Título:Fatty acyl recognition and transfer by an integral membrane -acyltransferase.
[So] Source:Science;359(6372), 2018 01 12.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DHHC (Asp-His-His-Cys) palmitoyltransferases are eukaryotic integral membrane enzymes that catalyze protein palmitoylation, which is important in a range of physiological processes, including small guanosine triphosphatase (GTPase) signaling, cell adhesion, and neuronal receptor scaffolding. We present crystal structures of two DHHC palmitoyltransferases and a covalent intermediate mimic. The active site resides at the membrane-cytosol interface, which allows the enzyme to catalyze thioester-exchange chemistry by using fatty acyl-coenzyme A and explains why membrane-proximal cysteines are candidates for palmitoylation. The acyl chain binds in a cavity formed by the transmembrane domain. We propose a mechanism for acyl chain-length selectivity in DHHC enzymes on the basis of cavity mutants with preferences for shorter and longer acyl chains.
[Mh] Termos MeSH primário: Acil Coenzima A/metabolismo
Aciltransferases/química
Proteínas de Peixe-Zebra/química
[Mh] Termos MeSH secundário: Aciltransferases/genética
Aciltransferases/metabolismo
Animais
Domínio Catalítico
Cristalização
Cristalografia por Raios X
Cisteína/química
Seres Humanos
Lipoilação
Modelos Moleculares
Mutação
Domínios Proteicos
Estrutura Secundária de Proteína
Especificidade por Substrato
Proteínas de Peixe-Zebra/genética
Proteínas de Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, N.I.H., INTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Acyl Coenzyme A); 0 (Zebrafish Proteins); EC 2.3.- (Acyltransferases); EC 2.3.- (ZDHHC20 protein, human); K848JZ4886 (Cysteine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


  4 / 9767 MEDLINE  
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[PMID]:29372791
[Au] Autor:Kiselev KV; Ogneva ZV; Suprun AR; Zhuravlev YN
[Ti] Título:[Expression of the stilbene synthase genes in the needles of spruce Picea jezoensis].
[So] Source:Genetika;52(11):1279-86, 2016 Nov.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Stilbenes are valuable plant phytoalexins, the biosynthesis of which is characteristic of different groups of phylogenetically unrelated plants. It is believed that all the stilbenes are the derivatives of resveratrol (3,5,4'-trihydroxy-trans-stilbene) or compounds close to it (pinosylvin or piceatannol). The last stage of the resveratrol biosynthesis takes place with the involvement of stilbene synthase or resveratrol synthase (STS). The family Pinaceae is characterized by the presence of the derivatives of pinosylvin (genus Pinus) and piceatannol (genus Picea), the biosynthetic pathways of which are scarcely examined. Previously, in different species of the genus Picea, only two stilbene synthase genes were described. On the basis of RNA isolated from the needles of spruce Picea jezoensis, the full-length cDNAs of the four stilbene synthase genes, PjSTS1a, PjSTS1b, PjSTS2, and PjSTS3, were obtained. Then, using the clone frequency analysis and real-time PCR, expression of the PjSTS1a, PjSTS1b, PjSTS2, and PjSTS3 genes was examined in the needles of P. jezoensis accessions of different age and sampled in different seasons (spring, summer, autumn, winter). Among the analyzed transcripts, the PjSTS1a and PjSTS1b genes were the most frequent, indicating their higher level of expression compared to other STS genes. The highest level of PjSTS1a and PjSTS1b expression was observed in autumn, while the level of PjSTS2 and PjSTS3 expression was the highest in spring and winter. Moreover, the highest PjSTS expression was detected in the young tissues of P. jezoensis in autumn, which may indicate a higher level of stilbene biosynthesis in these tissues.
[Mh] Termos MeSH primário: Aciltransferases/biossíntese
Regulação Enzimológica da Expressão Gênica/fisiologia
Regulação da Expressão Gênica de Plantas/fisiologia
Picea/enzimologia
Proteínas de Plantas/biossíntese
[Mh] Termos MeSH secundário: Aciltransferases/genética
Picea/genética
Proteínas de Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); EC 2.3.- (Acyltransferases); EC 2.3.1.- (stilbene synthase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE


  5 / 9767 MEDLINE  
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[PMID]:28470626
[Au] Autor:Sun L; Yuan Q; Xu T; Yao L; Feng J; Ma J; Wang L; Lv C; Wang D
[Ad] Endereço:Department of Nephrology, The First Affiliated Hospital of China Medical University, Shenyang, 110001, People's Republic of China.
[Ti] Título:Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice.
[So] Source:Biotechnol Lett;39(8):1159-1166, 2017 Aug.
[Is] ISSN:1573-6776
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To investigate the potential of interleukin (IL)-15 as a novel adjuvant for Mycobacterium tuberculosis (Mtb) antigen 85A (Ag85A) vaccine. RESULTS: C57BL/6 mice were intramuscularly immunized three times with a plasmid expressing the Ag85A-IL-15 fusion protein (pcDNA3.1-Ag85A-IL-15), with the empty pcDNA3.1 vector and the pcDNA3.1-Ag85A as control. Mice vaccinated with pcDNA3.1-Ag85A-IL-15 generated more secretory IgA (sIgA) into their lung (209 ± 21 µg/ml) and acquired an enhanced serum IgG response to Ag85A. IgG2a/IgG1 ratios were upregulated, natural killer cell activity was augmented and Ag85A-specific splenic T cell proliferation was enhanced in these mice as well. Vaccination with pcDNA3.1-Ag85A-IL-15 promoted the polarization of CD4 T cells towards a Th1 type in the spleen, and significantly upregulated the serum level of interferon (IFN)-γ (458 ± 98 pg/ml), a typical Th1 cytokine. IFN-γ-expressing CD8 cells were also increased in the spleen after pcDNA3.1-Ag85A-IL-15 immunization. CONCLUSIONS: A superior immune type I response in mice vaccinated with plasmid Ag85A-IL-15 has been achieved.
[Mh] Termos MeSH primário: Aciltransferases/imunologia
Adjuvantes Imunológicos
Antígenos de Bactérias/imunologia
Interferon gama/imunologia
Proteínas Recombinantes de Fusão/imunologia
Vacinas contra a Tuberculose/imunologia
Vacinas de DNA/imunologia
[Mh] Termos MeSH secundário: Aciltransferases/química
Aciltransferases/genética
Adjuvantes Imunológicos/química
Adjuvantes Imunológicos/genética
Animais
Antígenos de Bactérias/química
Antígenos de Bactérias/genética
Líquido da Lavagem Broncoalveolar/citologia
Células Cultivadas
Clonagem Molecular
Células HEK293
Seres Humanos
Interferon gama/química
Interferon gama/genética
Camundongos
Camundongos Endogâmicos C57BL
Plasmídeos
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/genética
Baço/citologia
Tuberculose
Vacinas contra a Tuberculose/química
Vacinas contra a Tuberculose/genética
Vacinas de DNA/química
Vacinas de DNA/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antigens, Bacterial); 0 (Recombinant Fusion Proteins); 0 (Tuberculosis Vaccines); 0 (Vaccines, DNA); 82115-62-6 (Interferon-gamma); EC 2.3.- (Acyltransferases); EC 2.3.1.- (antigen 85A, Mycobacterium tuberculosis)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s10529-017-2342-1


  6 / 9767 MEDLINE  
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[PMID]:29235335
[Au] Autor:Shmarakov IO; Borschovetska VL; Ivanishchuk LP; Marchenko MM
[Ti] Título:Hepatotoxicity of bisphenol A under conditions of differential supplementation with retinoids.
[So] Source:Ukr Biochem J;88(3):99-105, 2016 May-Jun.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Classical xenoestrogenic in vivo effects of bisphenol A (2,2-bis(4-hydroxyphenyl)propane, BPA) are well-described in the literature, however the molecular mechanisms of BPA-induced hepatotoxicity are not fully characterized. The work is aimed to assess biochemical markers of BPA induced hepatotoxicity under conditions of differential supplementation with retinoids. We demonstrate that the absence of hepatic retinyl esters as the main form of vitamin A storage provides for a resistance to BPA induced liver damage. Retinoid supplementation increases the hepatotoxic effects of bisphenol A, evidenced in higher indexes of oxidative damage of lipids, proteins and non-protein thiol groups as well as increase of serum alanine aminotransferase activity and myeloperoxidase activity in liver parenchyma. The absence of hepatotoxicity signs when hepatic retinoid stores are depleted and their presence during normal or excessive retinoid supplementation suggest that hepatic retinoid availability is one of the factors determining the hepatotoxicity of bisphenol A.
[Mh] Termos MeSH primário: Compostos Benzidrílicos/toxicidade
Doença Hepática Induzida por Substâncias e Drogas/metabolismo
Disruptores Endócrinos/toxicidade
Fenóis/toxicidade
Vitamina A/análogos & derivados
Vitamina A/efeitos adversos
[Mh] Termos MeSH secundário: Aciltransferases/deficiência
Aciltransferases/genética
Alanina Transaminase/sangue
Alanina Transaminase/genética
Animais
Doença Hepática Induzida por Substâncias e Drogas/patologia
Expressão Gênica
Fígado/efeitos dos fármacos
Fígado/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Estresse Oxidativo
Peroxidase/genética
Peroxidase/metabolismo
Carbonilação Proteica/efeitos dos fármacos
Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo
Vitamina A/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzhydryl Compounds); 0 (Endocrine Disruptors); 0 (Phenols); 0 (Thiobarbituric Acid Reactive Substances); 11103-57-4 (Vitamin A); 3LE3D9D6OY (retinol acetate); EC 1.11.1.7 (Peroxidase); EC 2.3.- (Acyltransferases); EC 2.3.1.- (lecithin-retinol acyltransferase); EC 2.6.1.2 (Alanine Transaminase); MLT3645I99 (bisphenol A)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.03.099


  7 / 9767 MEDLINE  
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[PMID]:28671269
[Au] Autor:Urciuoli E; Coletta I; Rizzuto E; De Vito R; Petrini S; D'Oria V; Pezzullo M; Milano GM; Cozza R; Locatelli F; Peruzzi B
[Ad] Endereço:Research Laboratories, Bambino Gesù Children's Hospital, Rome, Italy.
[Ti] Título:Src nuclear localization and its prognostic relevance in human osteosarcoma.
[So] Source:J Cell Physiol;233(2):1658-1670, 2018 Feb.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Osteosarcoma is the most common malignant bone tumor in children and young adults. The identification of proteins which exhibit different subcellular localization in low- versus high-risk osteosarcoma can be instrumental to obtain prognostic information and to develop innovative therapeutic strategies. Beside the well-characterized membrane and cytoplasmic localization of Src protein, this study evaluated the prognostic relevance of its so-far unknown nuclear compartmentalization. We analyzed the subcellular distribution of total and activated (pY418) Src in a tissue microarray including 60 osteosarcoma samples. Immunohistochemical analyses revealed a variable pattern of Src expression and localization, ranging from negative to high-stained nuclei combined with a substantial cytoplasmic staining for total and activated forms. The analysis of Kaplan-Meier survival curves in relationship to the diverse permutations of cytoplasmic and nuclear staining suggested a correlation between Src subcellular localization and the overall survival (OS) of osteosarcoma patients. In order to explain this different subcellular localization, normal osteoblasts and three osteosarcoma cell lines were used to investigate the molecular mechanism. Once confirmed a variable Src localization also in these cell lines, we demonstrated a correlation between the N-myristoyltransferase enzymes expression and activity and the Src nuclear content. In conclusion, these results described a so-far unknown Src nuclear localization in osteosarcoma cells, suggesting that the combined detection of nuclear and cytoplasmic Src levels can be used as a prognostic marker for osteosarcoma patient survival. A correlation between the N-myristoyltransferase enzymes and the Src subcellular localization was described as well.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo
Neoplasias Ósseas/enzimologia
Núcleo Celular/enzimologia
Osteossarcoma/enzimologia
Quinases da Família src/metabolismo
[Mh] Termos MeSH secundário: Aciltransferases/metabolismo
Adolescente
Adulto
Neoplasias Ósseas/mortalidade
Neoplasias Ósseas/patologia
Neoplasias Ósseas/terapia
Linhagem Celular Tumoral
Criança
Ativação Enzimática
Feminino
Seres Humanos
Estimativa de Kaplan-Meier
Masculino
Osteossarcoma/mortalidade
Osteossarcoma/patologia
Osteossarcoma/terapia
Prognóstico
Processamento de Proteína Pós-Traducional
Fatores de Tempo
Análise Serial de Tecidos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); EC 2.3.- (Acyltransferases); EC 2.3.1.97 (glycylpeptide N-tetradecanoyltransferase); EC 2.7.10.2 (src-Family Kinases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.26079


  8 / 9767 MEDLINE  
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[PMID]:29100095
[Au] Autor:Nguyen TTM; Murakami Y; Sheridan E; Ehresmann S; Rousseau J; St-Denis A; Chai G; Ajeawung NF; Fairbrother L; Reimschisel T; Bateman A; Berry-Kravis E; Xia F; Tardif J; Parry DA; Logan CV; Diggle C; Bennett CP; Hattingh L; Rosenfeld JA; Perry MS; Parker MJ; Le Deist F; Zaki MS; Ignatius E; Isohanni P; Lönnqvist T; Carroll CJ; Johnson CA; Gleeson JG; Kinoshita T; Campeau PM
[Ad] Endereço:Centre Hospitalier Universitaire Sainte Justine Research Center, University of Montreal, Montreal, QC H3T1C5, Canada.
[Ti] Título:Mutations in GPAA1, Encoding a GPI Transamidase Complex Protein, Cause Developmental Delay, Epilepsy, Cerebellar Atrophy, and Osteopenia.
[So] Source:Am J Hum Genet;101(5):856-865, 2017 Nov 02.
[Is] ISSN:1537-6605
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Approximately one in every 200 mammalian proteins is anchored to the cell membrane through a glycosylphosphatidylinositol (GPI) anchor. These proteins play important roles notably in neurological development and function. To date, more than 20 genes have been implicated in the biogenesis of GPI-anchored proteins. GPAA1 (glycosylphosphatidylinositol anchor attachment 1) is an essential component of the transamidase complex along with PIGK, PIGS, PIGT, and PIGU (phosphatidylinositol-glycan biosynthesis classes K, S, T, and U, respectively). This complex orchestrates the attachment of the GPI anchor to the C terminus of precursor proteins in the endoplasmic reticulum. Here, we report bi-allelic mutations in GPAA1 in ten individuals from five families. Using whole-exome sequencing, we identified two frameshift mutations (c.981_993del [p.Gln327Hisfs 102] and c.920delG [p.Gly307Alafs 11]), one intronic splicing mutation (c.1164+5C>T), and six missense mutations (c.152C>T [p.Ser51Leu], c.160_161delinsAA [p.Ala54Asn], c.527G>C [p.Trp176Ser], c.869T>C [p.Leu290Pro], c.872T>C [p.Leu291Pro], and c.1165G>C [p.Ala389Pro]). Most individuals presented with global developmental delay, hypotonia, early-onset seizures, cerebellar atrophy, and osteopenia. The splicing mutation was found to decrease GPAA1 mRNA. Moreover, flow-cytometry analysis of five available individual samples showed that several GPI-anchored proteins had decreased cell-surface abundance in leukocytes (FLAER, CD16, and CD59) or fibroblasts (CD73 and CD109). Transduction of fibroblasts with a lentivirus encoding the wild-type protein partially rescued the deficiency of GPI-anchored proteins. These findings highlight the role of the transamidase complex in the development and function of the cerebellum and the skeletal system.
[Mh] Termos MeSH primário: Aciltransferases/genética
Atrofia/genética
Doenças Ósseas Metabólicas/genética
Deficiências do Desenvolvimento/genética
Epilepsia/genética
Glicoproteínas de Membrana/genética
Mutação/genética
[Mh] Termos MeSH secundário: Adolescente
Adulto
Alelos
Cerebelo/patologia
Criança
Pré-Escolar
Exoma/genética
Feminino
Fibroblastos/patologia
Glicosilfosfatidilinositóis/genética
Seres Humanos
Masculino
Hipotonia Muscular/genética
Linhagem
RNA Mensageiro/genética
Convulsões/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (GPAA1 protein, human); 0 (Glycosylphosphatidylinositols); 0 (Membrane Glycoproteins); 0 (RNA, Messenger); EC 2.3.- (Acyltransferases); EC 2.3.2.- (COOH-terminal signal transamidase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171104
[St] Status:MEDLINE


  9 / 9767 MEDLINE  
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[PMID]:28966274
[Au] Autor:Zhong Y; Han X; Li S; Qi H; Song Y; Qiao X
[Ad] Endereço:Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University.
[Ti] Título:Design, Synthesis, Antifungal Activity and Molecular Docking of Thiochroman-4-one Derivatives.
[So] Source:Chem Pharm Bull (Tokyo);65(10):904-910, 2017.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:N-Myristoyltransferase (NMT) has been validated pre-clinically as a target for treatment of fungal infections. Various substituted thiochroman-4-one derivatives have been synthesized by an efficient method. The synthesized compounds 7a-y and 8a-t were evaluated for their in vitro antifungal activity against the Canidia albicans, Cryptococcus neoformans, Epidermophyton floccosum, Mucor racemosus, Microsporum gypseum and Aspergillus nigerstrain. A series of compounds exhibited significant activity (minimal inhibitory concentrotion (MIC)=0.5-16 µg/mL) against Canidia albicans and Cryptococcus neoformans. The antifungal activity of compound 7b was reached to that of fluconazole, which can serve as a good starting point for further studies of structural diversity of the NMT inhibitors. The molecular docking studies revealed an interesting binding profile with very high receptor affinity for NMT of Canidia albicans.
[Mh] Termos MeSH primário: Aciltransferases/metabolismo
Antifúngicos/síntese química
Cromanos/química
Desenho de Drogas
Inibidores Enzimáticos/síntese química
[Mh] Termos MeSH secundário: Aciltransferases/química
Antifúngicos/química
Antifúngicos/farmacologia
Sítios de Ligação
Candida albicans/efeitos dos fármacos
Domínio Catalítico
Cromanos/síntese química
Cromanos/farmacologia
Cryptococcus neoformans/efeitos dos fármacos
Inibidores Enzimáticos/química
Inibidores Enzimáticos/farmacologia
Fluconazol/farmacologia
Testes de Sensibilidade Microbiana
Simulação de Acoplamento Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Chromans); 0 (Enzyme Inhibitors); 8VZV102JFY (Fluconazole); EC 2.3.- (Acyltransferases); EC 2.3.1.97 (glycylpeptide N-tetradecanoyltransferase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c17-00274


  10 / 9767 MEDLINE  
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[PMID]:28890377
[Au] Autor:Wang Y; Dou Y; Wang R; Guan X; Hu Z; Zheng J
[Ad] Endereço:College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China.
[Ti] Título:Molecular characterization and functional analysis of chalcone synthase from Syringa oblata Lindl. in the flavonoid biosynthetic pathway.
[So] Source:Gene;635:16-23, 2017 Nov 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The flower color of Syringa oblata Lindl., which is often modulated by the flavonoid content, varies and is an important ornamental feature. Chalcone synthase (CHS) catalyzes the first key step in the flavonoid biosynthetic pathway. However, little is known about the role of S. oblata CHS (SoCHS) in flavonoid biosynthesis in this species. Here, we isolate and analyze the cDNA (SoCHS1) that encodes CHS in S. oblata. We also sought to analyzed the molecular characteristics and function of flavonoid metabolism by SoCHS1. We successfully isolated the CHS-encoding genomic DNA (gDNA) in S. oblata (SoCHS1), and the gene structural analysis indicated it had no intron. The opening reading frame (ORF) sequence of SoCHS1 was 1170bp long and encoded a 389-amino acid polypeptide. Multiple sequence alignment revealed that both the conserved CHS active site residues and CHS signature sequence were in the deduced amino acid sequence of SoCHS1. Crystallographic analysis revealed that the protein structure of SoCHS1 is highly similar to that of FnCHS1 in Freesia hybrida. The quantitative real-time polymerase chain reaction (PCR) performed to detect the SoCHS1 transcript expression levels in flowers, and other tissues revealed the expression was significantly correlated with anthocyanin accumulation during flower development. The ectopic expression results of Nicotiana tabacum showed that SoCHS1 overexpression in transgenic tobacco changed the flower color from pale pink to pink. In conclusion, these results suggest that SoCHS1 plays an essential role in flavonoid biosynthesis in S. oblata, and could be used to modify flavonoid components in other plant species.
[Mh] Termos MeSH primário: Aciltransferases/genética
Vias Biossintéticas
Flavonoides/metabolismo
Flores/genética
[Mh] Termos MeSH secundário: Aciltransferases/biossíntese
Sequência de Aminoácidos/genética
Antocianinas/metabolismo
Clonagem Molecular
Flavonoides/genética
Flores/crescimento & desenvolvimento
Regulação da Expressão Gênica de Plantas
Alinhamento de Sequência
Syringa/enzimologia
Syringa/genética
Tabaco/genética
Tabaco/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Flavonoids); EC 2.3.- (Acyltransferases); EC 2.3.1.74 (flavanone synthetase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE



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