[PMID]: | 28669930 |
[Au] Autor: | Ning T; Cui H; Sun F; Zou J |
[Ad] Endereço: | Department of Neurosurgery, Weihai Central Hospital, Shandong, China. |
[Ti] Título: | Systemic analysis of genome-wide expression profiles identified potential therapeutic targets of demethylation drugs for glioblastoma. |
[So] Source: | Gene;627:387-392, 2017 Sep 05. |
[Is] ISSN: | 1879-0038 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | Glioblastoma represents one of the most aggressive malignant brain tumors with high morbidity and motility. Demethylation drugs have been developed for its treatment with little efficacy has been observed. The purpose of this study was to screen therapeutic targets of demethylation drugs or bioactive molecules for glioblastoma through systemic bioinformatics analysis. We firstly downloaded genome-wide expression profiles from the Gene Expression Omnibus (GEO) and conducted the primary analysis through R software, mainly including preprocessing of raw microarray data, transformation between probe ID and gene symbol and identification of differential expression genes (DEGs). Secondly, functional enrichment analysis was conducted via the Database for Annotation, Visualization and Integrated Discovery (DAVID) to explore biological processes involved in the development of glioblastoma. Thirdly, we constructed protein-protein interaction (PPI) network of interested genes and conducted cross analysis for multi datasets to obtain potential therapeutic targets for glioblastoma. Finally, we further confirmed the therapeutic targets through real-time RT-PCR. As a result, biological processes that related to cancer development, amino metabolism, immune response and etc. were found to be significantly enriched in genes that differential expression in glioblastoma and regulated by 5'aza-dC. Besides, network and cross analysis identified ACAT2, UFC1 and CYB5R1 as novel therapeutic targets of demethylation drugs which also confirmed by real time RT-PCR. In conclusions, our study identified several biological processes and genes that involved in the development of glioblastoma and regulated by 5'aza-dC, which would be helpful for the treatment of glioblastoma. |
[Mh] Termos MeSH primário: |
Antineoplásicos/farmacologia Neoplasias Encefálicas/genética Metilação de DNA/efeitos dos fármacos Inibidores Enzimáticos/farmacologia Regulação Neoplásica da Expressão Gênica Glioblastoma/genética
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[Mh] Termos MeSH secundário: |
Citocromo-B(5) Redutase/genética Redes Reguladoras de Genes Genoma Humano Seres Humanos Mapas de Interação de Proteínas Esterol O-Aciltransferase/genética Enzimas de Conjugação de Ubiquitina/genética
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antineoplastic Agents); 0 (Enzyme Inhibitors); 0 (UFC1 protein, human); EC 1.6.2.2 (Cytochrome-B(5) Reductase); EC 2.3.1.26 (Sterol O-Acyltransferase); EC 2.3.1.26 (sterol O-acyltransferase 2); EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes) |
[Em] Mês de entrada: | 1709 |
[Cu] Atualização por classe: | 170920 |
[Lr] Data última revisão:
| 170920 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170704 |
[St] Status: | MEDLINE |
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