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  1 / 2107 MEDLINE  
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[PMID]:28781260
[Au] Autor:Pogorelova TN; Gunko VO; Avrutskaya VV; Kaushanskaya LV; Durnitsyna OA
[Ad] Endereço:Rostov Scientific-Research Institute of Obstetrics and Pediatrics.
[Ti] Título:[Impairments of placental amino acid metabolism in fetal growth restriction].
[Ti] Título:Narushenie platsentarnogo obmena aminokislot pri zaderzhke rosta ploda..
[So] Source:Biomed Khim;63(3):266-271, 2017 May.
[Is] ISSN:2310-6972
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The content of the amino acids in the placenta during physiological pregnancy and fetal growth restriction (FGR) has been investigated my means of the method of ion-exchange chromatography. It has been found that in FGR the placental amino acid pool is characterized by a decreased content of arginine, proline, alanine, serine, cysteine, methionine, tryptophan, leucine, threonine, tyrosine, phenylalanine, glutamine and an increased content of dicarboxylic amino acids, lysine, histidine and glycine. These changes are accompanied by altered activity of some enzymes of amino acid metabolism, and the degree of these changes correlates with the level of corresponding amino acids.
[Mh] Termos MeSH primário: Aminoácidos/metabolismo
Retardo do Crescimento Fetal/metabolismo
Recém-Nascido de Baixo Peso
Placenta/metabolismo
[Mh] Termos MeSH secundário: Adulto
Alanina Transaminase/metabolismo
Arginase/metabolismo
Aspartato Aminotransferases/metabolismo
Estudos de Casos e Controles
Feminino
Desenvolvimento Fetal/fisiologia
Retardo do Crescimento Fetal/patologia
Glutamato Sintase/metabolismo
Homeostase
Seres Humanos
Recém-Nascido
Troca Materno-Fetal/fisiologia
Placenta/química
Placenta/patologia
Gravidez
Transaminases/metabolismo
Tirosina Transaminase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); EC 1.4.1.13 (Glutamate Synthase); EC 2.6.1.- (Transaminases); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase); EC 2.6.1.3 (cysteine aminotransferase); EC 2.6.1.5 (Tyrosine Transaminase); EC 3.5.3.1 (Arginase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE
[do] DOI:10.18097/PBMC20176303266


  2 / 2107 MEDLINE  
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[PMID]:28362875
[Au] Autor:Hou F; Li S; Wang J; Kang X; Weng Y; Xing G
[Ad] Endereço:Horticulture College, Shanxi Agricultural University, Taigu, China.
[Ti] Título:Identification and validation of reference genes for quantitative real-time PCR studies in long yellow daylily, Hemerocallis citrina Borani.
[So] Source:PLoS One;12(3):e0174933, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gene expression analysis using reverse transcription quantitative real-time PCR (RT-qPCR) requires the use of reference gene(s) in the target species. The long yellow daylily, Hemerocallis citrina Baroni. is rich in beneficial secondary metabolites and is considered as a functional vegetable. It is widely cultivated and consumed in East Asian countries. However, reference genes for use in RT-qPCR in H. citrina are not available. In the present study, six potential reference genes, actin (ACT), AP-4 complex subunit (AP4), tubulin (TUB), ubiquitin (UBQ), 18S and 60S ribosomal RNA, were selected and their expression stability in different developmental stages, organs and accessions was evaluated using four statistical software packages (geNorm, NormFinder, BestKeeper, and RefFinder). For commercial flower buds of different landraces, the combination of 60S, TUB, and AP4 was appropriate whereas ACT and 60S was suitable for normalization of different organs. In addition, AP4 exhibited the most stable expression in flower buds among different developmental stages. UBQ was less stable than the other reference genes under the experimental conditions except under different organs was 18S. The relative expression levels of two genes, primary-amine oxidase (HcAOC3) and tyrosine aminotransferase (HcTAT) which play important roles in alkaloid biosynthesis were also examined in different organs of the 'Datong' landrace, which further confirmed the results of selected reference genes. This is the first report to evaluate the stability of reference genes in the long yellow daylily that can serve as a foundation for RT-qPCR analysis of gene expression in this species.
[Mh] Termos MeSH primário: Hemerocallis/genética
Proteínas de Plantas/genética
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas/genética
Software
Tubulina (Proteína)/genética
Tirosina Transaminase/genética
Ubiquitina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Tubulin); 0 (Ubiquitin); EC 2.6.1.5 (Tyrosine Transaminase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174933


  3 / 2107 MEDLINE  
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[PMID]:27726859
[Au] Autor:Wang M; Toda K; Maeda HA
[Ad] Endereço:Department of Botany, University of Wisconsin-Madison, Madison, WI 53706, USA. Electronic address: mwang36@wisc.edu.
[Ti] Título:Biochemical properties and subcellular localization of tyrosine aminotransferases in Arabidopsis thaliana.
[So] Source:Phytochemistry;132:16-25, 2016 Dec.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plants produce various L-tyrosine (Tyr)-derived compounds that are of pharmaceutical or nutritional importance to humans. Tyr aminotransferase (TAT) catalyzes the reversible transamination between Tyr and 4-hydroxyphenylpyruvate (HPP), the initial step in the biosynthesis of many Tyr-derived plant natural products. Herein reported is the biochemical characterization and subcellular localization of TAT enzymes from the model plant Arabidopsis thaliana. Phylogenetic analysis showed that Arabidopsis has at least two homologous TAT genes, At5g53970 (AtTAT1) and At5g36160 (AtTAT2). Their recombinant enzymes showed distinct biochemical properties: AtTAT1 had the highest activity towards Tyr, while AtTAT2 exhibited a broad substrate specificity for both amino and keto acid substrates. Also, AtTAT1 favored the direction of Tyr deamination to HPP, whereas AtTAT2 preferred transamination of HPP to Tyr. Subcellular localization analysis using GFP-fusion proteins and confocal microscopy showed that AtTAT1, AtTAT2, and HPP dioxygenase (HPPD), which catalyzes the subsequent step of TAT, are localized in the cytosol, unlike plastid-localized Tyr and tocopherol biosynthetic enzymes. Furthermore, subcellular fractionation indicated that, while HPPD activity is restricted to the cytosol, TAT activity is detected in both cytosolic and plastidic fractions of Arabidopsis leaf tissue, suggesting that an unknown aminotransferase(s) having TAT activity is also present in the plastids. Biochemical and cellular analyses of Arabidopsis TATs provide a fundamental basis for future in vivo studies and metabolic engineering for enhanced production of Tyr-derived phytochemicals in plants.
[Mh] Termos MeSH primário: Arabidopsis
Tirosina Transaminase/metabolismo
Tirosina/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/química
Arabidopsis/enzimologia
Arabidopsis/genética
Dioxigenases/metabolismo
Seres Humanos
Cinética
Estrutura Molecular
Ácidos Fenilpirúvicos
Filogenia
Folhas de Planta/metabolismo
Plastídeos/metabolismo
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
Transaminases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenylpyruvic Acids); 0 (Recombinant Proteins); 156-39-8 (4-hydroxyphenylpyruvic acid); 42HK56048U (Tyrosine); EC 1.13.11.- (Dioxygenases); EC 2.6.1.- (Transaminases); EC 2.6.1.5 (Tyrosine Transaminase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161012
[St] Status:MEDLINE


  4 / 2107 MEDLINE  
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[PMID]:27411382
[Au] Autor:Nandi SS; Zheng H; Sharma NM; Shahshahan HR; Patel KP; Mishra PK
[Ad] Endereço:Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE.
[Ti] Título:Lack of miR-133a Decreases Contractility of Diabetic Hearts: A Role for Novel Cross Talk Between Tyrosine Aminotransferase and Tyrosine Hydroxylase.
[So] Source:Diabetes;65(10):3075-90, 2016 Oct.
[Is] ISSN:1939-327X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:MicroRNAs (miRNAs) have a fundamental role in diabetic heart failure. The cardioprotective miRNA-133a (miR-133a) is downregulated, and contractility is decreased in diabetic hearts. Norepinephrine (NE) is a key catecholamine that stimulates contractility by activating ß-adrenergic receptors (ß-AR). NE is synthesized from tyrosine by the rate-limiting enzyme, tyrosine hydroxylase (TH), and tyrosine is catabolized by tyrosine aminotransferase (TAT). However, the cross talk/link between TAT and TH in the heart is unclear. To determine whether miR-133a plays a role in the cross talk between TH and TAT and regulates contractility by influencing NE biosynthesis and/or ß-AR levels in diabetic hearts, Sprague-Dawley rats and miR-133a transgenic (miR-133aTg) mice were injected with streptozotocin to induce diabetes. The diabetic rats were then treated with miR-133a mimic or scrambled miRNA. Our results revealed that miR-133a mimic treatment improved the contractility of the diabetic rat's heart concomitant with upregulation of TH, cardiac NE, ß-AR, and downregulation of TAT and plasma levels of NE. In miR-133aTg mice, cardiac-specific miR-133a overexpression prevented upregulation of TAT and suppression of TH in the heart after streptozotocin was administered. Moreover, miR-133a overexpression in CATH.a neuronal cells suppressed TAT with concomitant upregulation of TH, whereas knockdown and overexpression of TAT demonstrated that TAT inhibited TH. Luciferase reporter assay confirmed that miR-133a targets TAT. In conclusion, miR-133a controls the contractility of diabetic hearts by targeting TAT, regulating NE biosynthesis, and consequently, ß-AR and cardiac function.
[Mh] Termos MeSH primário: MicroRNAs/metabolismo
Contração Miocárdica/fisiologia
Miocárdio/metabolismo
Tirosina 3-Mono-Oxigenase/metabolismo
Tirosina Transaminase/metabolismo
[Mh] Termos MeSH secundário: Animais
Western Blotting
Diabetes Mellitus Experimental/metabolismo
Células HEK293
Hemodinâmica/fisiologia
Seres Humanos
Imuno-Histoquímica
Masculino
Camundongos Transgênicos
MicroRNAs/genética
MicroRNAs/fisiologia
Contração Miocárdica/genética
Miócitos Cardíacos/metabolismo
Miócitos Cardíacos/fisiologia
Norepinefrina/metabolismo
Ratos
Ratos Sprague-Dawley
Receptores Adrenérgicos beta/genética
Receptores Adrenérgicos beta/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tirosina 3-Mono-Oxigenase/genética
Tirosina Transaminase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MIRN133 microRNA, rat); 0 (MicroRNAs); 0 (Receptors, Adrenergic, beta); EC 1.14.16.2 (Tyrosine 3-Monooxygenase); EC 2.6.1.5 (Tyrosine Transaminase); X4W3ENH1CV (Norepinephrine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171001
[Lr] Data última revisão:
171001
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160715
[St] Status:MEDLINE
[do] DOI:10.2337/db16-0023


  5 / 2107 MEDLINE  
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[PMID]:27400061
[Au] Autor:Scoccianti V; Bucchini AE; Iacobucci M; Ruiz KB; Biondi S
[Ad] Endereço:Dipartimento di Scienze Biomolecolari, Università di Urbino Carlo Bo, via Bramante 28, 61029 Urbino, Italy.
[Ti] Título:Oxidative stress and antioxidant responses to increasing concentrations of trivalent chromium in the Andean crop species Chenopodium quinoa Willd.
[So] Source:Ecotoxicol Environ Saf;133:25-35, 2016 Nov.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Quinoa (Chenopodium quinoa Willd), an ancient Andean seed crop, exhibits exceptional nutritional properties and resistance to abiotic stress. The species' tolerance to heavy metals has, however, not yet been investigated nor its ability to take up and translocate chromium (Cr). This study aimed to investigate the metabolic adjustments occurring upon exposure of quinoa to several concentrations (0.01-5mM) of CrCl3. Young hydroponically grown plants were used to evaluate Cr uptake, growth, oxidative stress, and other biochemical parameters three and/or seven days after treatment. Leaves accumulated the lowest amounts of Cr, while roots and stems accumulated the most at low and at high metal concentrations, respectively. Fresh weight and photosynthetic pigments were reduced only by the higher Cr(III) doses. Substantially increased lipid peroxidation, hydrogen peroxide, and proline levels were observed only with 5mM Cr(III). Except for a significant decrease at day 7 with 5mM Cr(III), total polyphenols and flavonoids maintained control levels in Cr(III)-treated plants, whereas antioxidant activity increased in a dose-dependent manner. Maximum polyamine accumulation was observed in 1mM CrCl3-treated plants. Even though α- and γ-tocopherols also showed enhanced levels only with the 1mM concentration, tyrosine aminotransferase (TAT, EC 2.6.1.5) activity increased under Cr(III) treatment in a dose- and time-dependent manner. Taken together, results suggest that polyamines, tocopherols, and TAT activity could contribute to tolerance to 1mM Cr(III), but not to the highest concentration that, instead, generated oxidative stress.
[Mh] Termos MeSH primário: Antioxidantes/análise
Chenopodium quinoa/efeitos dos fármacos
Cromo/toxicidade
Estresse Oxidativo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Chenopodium quinoa/metabolismo
Relação Dose-Resposta a Droga
Flavonoides/análise
Flavonoides/metabolismo
Peróxido de Hidrogênio/análise
Peroxidação de Lipídeos/efeitos dos fármacos
Oxirredução
Fotossíntese/efeitos dos fármacos
Folhas de Planta/metabolismo
Raízes de Plantas/metabolismo
Caules de Planta/metabolismo
Poliaminas/análise
Polifenóis/análise
Prolina/análise
Sementes/metabolismo
Tocoferóis/análise
Tirosina Transaminase/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Flavonoids); 0 (Polyamines); 0 (Polyphenols); 0R0008Q3JB (Chromium); 9DLQ4CIU6V (Proline); BBX060AN9V (Hydrogen Peroxide); EC 2.6.1.5 (Tyrosine Transaminase); R0ZB2556P8 (Tocopherols)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160712
[St] Status:MEDLINE


  6 / 2107 MEDLINE  
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[PMID]:27382772
[Au] Autor:Shen L; Ren J; Jin W; Wang R; Ni C; Tong M; Liang Z; Yang D
[Ti] Título:[Role of NO signal in ABA-induced phenolic acids accumulation in Salvia miltiorrhiza hairy roots].
[So] Source:Sheng Wu Gong Cheng Xue Bao;32(2):222-30, 2016 Feb.
[Is] ISSN:1000-3061
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To investigate roles of nitric oxide (NO) signal in accumulations of phenolic acids in abscisic.acid (ABA)-induced Salvia miltiorrhiza hairy roots, S. miltiorrhiza hairy roots were treated with different concentrations of sodium nitroprusside (SNP)-an exogenous NO donor, for 6 days, and contents of phenolic acids in the hairy roots are determined. Then with treatment of ABA and NO scavenger (2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethylimidazoline-1- oxyl-3-oxide, c-PTIO) or NO synthase inhibitor (NG-nitro-L-arginine methyl ester, L-NAME), contents of phenolic acids and expression levels of three key genes involved in phenolic acids biosynthesis were detected. Phenolic acids production in S. miltiorrhiza hairy roots was most significantly improved by 100 µmoL/L SNP. Contents of RA and salvianolic acid B increased by 3 and 4 folds. ABA significantly improved transcript levels of PAL (phenylalanine ammonia lyase), TAT (tyrosine aminotransferase) and RAS (rosmarinic acid synthase), and increased phenolic acids accumulations. However, with treatments of ABA+c-PTIO or ABA+L-NAME, accumulations of phenolic acids and expression levels of the three key genes were significantly inhibited. Both NO and ABA can increase accumulations of phenolic acids in S. miltiorrhiza hairy roots. NO signal probably mediates the ABA-induced phenolic acids production.
[Mh] Termos MeSH primário: Ácido Abscísico/farmacologia
Hidroxibenzoatos/metabolismo
Óxido Nítrico/metabolismo
Raízes de Plantas/metabolismo
Salvia miltiorrhiza/metabolismo
[Mh] Termos MeSH secundário: Benzofuranos/metabolismo
Depuradores de Radicais Livres/farmacologia
Fenilalanina Amônia-Liase/metabolismo
Tirosina Transaminase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzofurans); 0 (Free Radical Scavengers); 0 (Hydroxybenzoates); 29656-58-4 (phenolic acid); 31C4KY9ESH (Nitric Oxide); 72S9A8J5GW (Abscisic Acid); C1GQ844199 (salvianolic acid B); EC 2.6.1.5 (Tyrosine Transaminase); EC 4.3.1.24 (Phenylalanine Ammonia-Lyase)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160708
[St] Status:MEDLINE


  7 / 2107 MEDLINE  
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[PMID]:27285949
[Au] Autor:Gokay S; Kendirci M; Ustkoyuncu PS; Kardas F; Bayram AK; Per H; Poyrazoglu HG
[Ad] Endereço:Division of Pediatric Nutrition and Metabolism, Department of Pediatrics, Faculty of Medicine, Erciyes University, Kayseri, Turkey. songulsa@yahoo.com.
[Ti] Título:Tyrosinemia type II: Novel mutations in TAT in a boy with unusual presentation.
[So] Source:Pediatr Int;58(10):1069-1072, 2016 Oct.
[Is] ISSN:1442-200X
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Tyrosinemia type II is a rare autosomal recessive disorder caused by deficiency of tyrosine aminotransferase (TAT). It may occur with ocular and cutaneous symptoms with or without mental retardation, but epileptic seizure is a rare presentation of this disease. Herein we report the clinical, biochemical and genetic features of a 4-year-old boy who presented with afebrile seizure and photophobia. Genomic DNA was obtained from peripheral blood leukocytes from the whole family. Sequencing analysis was performed using the MiSeq next-generation sequencing platform. Sequencing of TAT indicated two new homozygous mutations p.L312P (c.935T>C) and p.T408M (c.1223C>T) for the proband and his asymptomatic sister. During a 2 year follow-up period, the patient had overall poor compliance with protein-restricted diet, but his asymptomatic sister had good compliance with the diet. Cognitive function of the patient worsened steadily, but his asymptomatic sister maintained normal mental status. Tyrosinemia type II should be considered in the differential diagnosis of children presenting with epileptic seizure and photophobia; furthermore, early diagnosis and protein-restricted regimen are important to reduce the risk of long-term complications of tyrosinemia type II such as mental disability.
[Mh] Termos MeSH primário: DNA/genética
Mutação
Tirosina Transaminase/genética
Tirosinemias/genética
[Mh] Termos MeSH secundário: Pré-Escolar
Análise Mutacional de DNA
Homozigoto
Seres Humanos
Masculino
Linhagem
Tirosina Transaminase/metabolismo
Tirosinemias/enzimologia
[Pt] Tipo de publicação:CASE REPORTS
[Nm] Nome de substância:
9007-49-2 (DNA); EC 2.6.1.5 (Tyrosine Transaminase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160611
[St] Status:MEDLINE
[do] DOI:10.1111/ped.13062


  8 / 2107 MEDLINE  
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[PMID]:25997084
[Au] Autor:Diepeveen LA; Watson ME; McSpadden SB; Strauss RP; Callus BA; Yeoh GC
[Ad] Endereço:1 The Centre for Medical Research, Harry Perkins Institute of Medical Research , Nedlands, WA, Australia .
[Ti] Título:Epigenetic Modulators Enhance Constitutive and Liver-Specific Reporter Expression in Murine Liver Progenitor Cell Lines.
[So] Source:Tissue Eng Part C Methods;21(10):1080-7, 2015 Oct.
[Is] ISSN:1937-3392
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Stem cells expressing reporter constructs are extremely useful for their tracking in vivo or for determining cell lineage fate in vivo and in vitro. We generated liver progenitor cell (LPC) lines from actin-EGFP and TAT-GRE-lacZ mice. LPCs from the actin-EGFP mouse facilitate cell tracing following transplant as the reporter is constitutively expressed. LPCs from the TAT-GRE-lacZ mouse express ß-galactosidase under the control of the tyrosine aminotransferase (TAT) promoter and are only active in mature hepatocytes. We found that the utility of such LPC lines becomes severely limited by downregulation of transgene expression following extended culture. We show that epigenetic mechanisms are responsible for suppressing expression of both transgenes. Enhancement of transgene expression in both LPC lines was achieved by treating the cell lines with either the histone acetylating agent sodium butyrate or the DNA demethylating agent 5-azacytidine.
[Mh] Termos MeSH primário: Diferenciação Celular
Epigênese Genética
Hepatócitos/metabolismo
Fígado/metabolismo
Células-Tronco/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Genes Reporter
Fígado/citologia
Camundongos
Especificidade de Órgãos
Células-Tronco/citologia
Tirosina Transaminase
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 2.6.1.5 (Tyrosine Transaminase)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:151002
[Lr] Data última revisão:
151002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150522
[St] Status:MEDLINE
[do] DOI:10.1089/ten.TEC.2015.0131


  9 / 2107 MEDLINE  
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[PMID]:25975647
[Au] Autor:He Y; Cui J; He T; Bi Y
[Ad] Endereço:Department of Pediatric Surgery, The Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
[Ti] Título:5-azacytidine promotes terminal differentiation of hepatic progenitor cells.
[So] Source:Mol Med Rep;12(2):2872-8, 2015 Aug.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:5-azacytidine (5-azaC) is known to induce cardiomyocyte differentiation. However, its function in hepatocyte differentiation is unclear. The present study investigated the in vitro capability of 5-azaC to promote maturation and differentiation of mouse embryonic hepatic progenitor cells, with the aim of developing an approach for improving hepatic differentiation. Mouse embryonic hepatic progenitor cells (HP14.5 cells) were treated with 5-azaC at concentrations from 0 to 20 µmol/l, in addition to hepatocyte induction culture medium. Hepatocyte induction medium induces HP14.5 cell differentiation. 5-azaC may enhance the albumin promotor-driven Gaussia luciferase (ALB-GLuc) activity in induced HP14.5 cells. In the present study 2 µmol/l was found to be the optimum concentration with which to achieve this. The expression of hepatocyte-associated factors was not significantly different between the group treated with 5-azaC alone and the control group. The mRNA levels of ALB; cytokeratin 18 (CK18); tyrosine aminotransferase (TAT); and cytochrome p450, family 1, member A1 (CYP1A1); in addition to the protein levels of ALB, CK18 and uridine diphosphate glucuronyltransferase 1A (UGT1A) in the induced group with 5-azaC, were higher than those in the induced group without 5-azaC, although no significant differences were detected in expression of the hepatic stem cell markers, DLK and α-fetoprotein, between the two groups. Treatment with 5-azaC alone did not affect glycogen synthesis or indocyanine green (ICG) metabolic function in HP14.5 cells, although it significantly increased ICG uptake and periodic acid-Schiff-positive cell numbers amongst HP14.5 cells. Therefore, the present study demonstrated that treatment with 5-azaC alone exerted no effects on the maturation and differentiation of HP14.5 cells. However, 5-azaC exhibited a synergistic effect on the terminal differentiation of induced hepatic progenitor cells in association with a hepatic induction medium.
[Mh] Termos MeSH primário: Azacitidina/farmacologia
Diferenciação Celular/efeitos dos fármacos
Hepatócitos/citologia
Células-Tronco/citologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Citocromo P-450 CYP1A1/genética
Citocromo P-450 CYP1A1/metabolismo
Embrião de Mamíferos/citologia
Glucuronosiltransferase/metabolismo
Peptídeos e Proteínas de Sinalização Intercelular/metabolismo
Queratina-18/genética
Queratina-18/metabolismo
Camundongos
RNA Mensageiro/metabolismo
Células-Tronco/efeitos dos fármacos
Células-Tronco/metabolismo
Tirosina Transaminase/genética
Tirosina Transaminase/metabolismo
alfa-Fetoproteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dlk1 protein, mouse); 0 (Intercellular Signaling Peptides and Proteins); 0 (Keratin-18); 0 (RNA, Messenger); 0 (alpha-Fetoproteins); EC 1.14.14.1 (Cytochrome P-450 CYP1A1); EC 2.4.1.- (UGT1A1 enzyme); EC 2.4.1.17 (Glucuronosyltransferase); EC 2.6.1.5 (Tyrosine Transaminase); M801H13NRU (Azacitidine)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150611
[Lr] Data última revisão:
150611
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150516
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2015.3772


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[PMID]:25894779
[Au] Autor:Morozkova TS; Bogdanova LA; Semenov DE; Zhukova NA; Popova NA
[Ad] Endereço:Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia, pathol@inbox.ru.
[Ti] Título:PT/Y Mice Are Sensitive to the Inhibitory Effect of o-Aminoazotoluene on Glucocorticoid Induction of Tyrosine Aminotransferase and Its Hepatocarcinogenic Effect.
[So] Source:Bull Exp Biol Med;158(6):789-93, 2015 Apr.
[Is] ISSN:1573-8221
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PT/Y mice used for studies of the effects of mutagens are characterized by the absence of spontaneous tumors of the liver, but often develop these tumors in response to chronic oaminoazotoluene treatment. The level of glucocorticoid induction of adaptive hepatic enzyme tyrosine aminotransferase decreases by more than 70% 24 h after acute injection of o-aminoazotoluene to these animals. These mice can serve as a model for studies of the relationship between the effect of carcinogens on the regulation of activity of adaptive hepatic enzymes and their capacity to induce the development of liver tumors.
[Mh] Termos MeSH primário: Glucocorticoides/farmacologia
Fígado/metabolismo
Tirosina Transaminase/metabolismo
o-Aminoazotolueno/toxicidade
[Mh] Termos MeSH secundário: Animais
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucocorticoids); EC 2.6.1.5 (Tyrosine Transaminase); QHZ900P7ZA (o-Aminoazotoluene)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150506
[Lr] Data última revisão:
150506
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150421
[St] Status:MEDLINE
[do] DOI:10.1007/s10517-015-2863-3



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