Base de dados : MEDLINE
Pesquisa : D08.811.913.696.445.735.270.762 [Categoria DeCS]
Referências encontradas : 7708 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 771 ir para página                         

  1 / 7708 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29348663
[Au] Autor:Miao Y; Ajami NE; Huang TS; Lin FM; Lou CH; Wang YT; Li S; Kang J; Munkacsi H; Maurya MR; Gupta S; Chien S; Subramaniam S; Chen Z
[Ad] Endereço:Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope, 1500 Duarte Rd., Duarte, CA, 91010, USA.
[Ti] Título:Enhancer-associated long non-coding RNA LEENE regulates endothelial nitric oxide synthase and endothelial function.
[So] Source:Nat Commun;9(1):292, 2018 01 18.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The optimal expression of endothelial nitric oxide synthase (eNOS), the hallmark of endothelial homeostasis, is vital to vascular function. Dynamically regulated by various stimuli, eNOS expression is modulated at transcriptional, post-transcriptional, and post-translational levels. However, epigenetic modulations of eNOS, particularly through long non-coding RNAs (lncRNAs) and chromatin remodeling, remain to be explored. Here we identify an enhancer-associated lncRNA that enhances eNOS expression (LEENE). Combining RNA-sequencing and chromatin conformation capture methods, we demonstrate that LEENE is co-regulated with eNOS and that its enhancer resides in proximity to eNOS promoter in endothelial cells (ECs). Gain- and Loss-of-function of LEENE differentially regulate eNOS expression and EC function. Mechanistically, LEENE facilitates the recruitment of RNA Pol II to the eNOS promoter to enhance eNOS nascent RNA transcription. Our findings unravel a new layer in eNOS regulation and provide novel insights into cardiovascular regulation involving endothelial function.
[Mh] Termos MeSH primário: Células Endoteliais/metabolismo
Elementos Facilitadores Genéticos/genética
Regulação Enzimológica da Expressão Gênica
Óxido Nítrico Sintase Tipo III/genética
RNA Longo não Codificante/genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Perfilação da Expressão Gênica
Seres Humanos
Masculino
Camundongos Endogâmicos C57BL
Óxido Nítrico Sintase Tipo III/metabolismo
Regiões Promotoras Genéticas/genética
RNA Polimerase II/metabolismo
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Long Noncoding); 0 (long non-coding RNA LEENE, human); EC 1.14.13.39 (Nitric Oxide Synthase Type III); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02113-y


  2 / 7708 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29291460
[Au] Autor:Choi JH; Lee S; Nah JY; Kim HK; Paek JS; Lee S; Ham H; Hong SK; Yun SH; Lee T
[Ad] Endereço:Microbial Safety Team, National Institute of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea.
[Ti] Título:Species composition of and fumonisin production by the Fusarium fujikuroi species complex isolated from Korean cereals.
[So] Source:Int J Food Microbiol;267:62-69, 2018 Feb 21.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To assess the risk of fumonisin contamination in Korean cereals, we isolated colonies of the Fusarium fujikuroi species complex (FFSC) from barley, maize, rice and soybean samples from 2011 to 2015. A total of 878 FFSC strains were isolated mostly from maize and rice, and species identity of the isolates were determined using the DNA sequence of the translation elongation factor 1-α (TEF-1α) and RNA polymerase II (RPB2) genes. Fusaria recovered from Korean cereals included F. fujikuroi (317 isolates and a frequency of 36%), F. proliferatum (212 isolates and 24.1%), F. verticillioides (170 isolates and 19.4%), F. concentricum (86 strains and 9.8%), F. andiyazi (56 isolates and 6.4%), F. subglutinans (28 isolates and 3.2%), F. thapsinum (5 isolates and 0.6%), and F. circinatum (2 isolates and 0.2%). The rice samples were dominated by F. fujikuroi (47.4%), F. proliferatum (27.3%), and F. concentricum (15.1%), whereas maize samples were dominated by F. verticillioides (33.9%), F. fujikuroi (25.3%), and F. proliferatum (21.1%). A phylogenetic analysis of 70 representative isolates demonstrated that each species was resolved as genealogically exclusive in the ML tree. Fumonisin production potential was evaluated using a PCR assay for the fumonisin biosynthesis gene, FUM1 in all of the isolates. Most of the isolates tested (94%) were positive for FUM1. All of the isolates assigned to F. fujikuroi, F. proliferatum, F. verticillioides and F. thapsinum were positive for FUM1 irrespective of their host origin. Seventy-seven representative isolates positive for FUM1 were examined for fumonisin production in rice medium. The majority of F. proliferatum (26/27, 96.3%), F. verticillioides (16/17, 94.1%) and F. fujikuroi (19/25, 76.0%) produced both FB and FB . Notably, 16 of 19 fumonisin-producing F. fujikuroi produced >1000µg/g of fumonisins (FB +FB ) in rice medium, which is higher than that in previous reports. These results suggest that F. fujikuroi can produce high levels of fumonisins similar to F. verticillioides and F. proliferatum.
[Mh] Termos MeSH primário: Grãos Comestíveis/química
Grãos Comestíveis/microbiologia
Fumonisinas/química
Fusarium/isolamento & purificação
[Mh] Termos MeSH secundário: Biodiversidade
Fumonisinas/análise
Fumonisinas/metabolismo
Fusarium/classificação
Fusarium/genética
Fator 1 de Elongação de Peptídeos/genética
Filogenia
RNA Polimerase II/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fumonisins); 0 (Peptide Elongation Factor 1); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180102
[St] Status:MEDLINE


  3 / 7708 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28453430
[Au] Autor:Segala G; Picard D
[Ad] Endereço:a Département de Biologie Cellulaire , Université de Genève , Genève , Switzerland.
[Ti] Título:H2B monoubiquitination: t'ub or not t'ub for inducible enhancers.
[So] Source:Transcription;8(2):126-132, 2017 03 15.
[Is] ISSN:2154-1272
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recently, we reported the unexpected finding that the monoubiquitination of histone H2B (H2Bub1) regulates inducible enhancers. Here, we propose a conceptual framework to reconcile the apparently discrepant roles of H2Bub1 in transcription initiation and elongation, and we discuss how H2Bub1 could regulate cellular processes linked to non-coding transcription.
[Mh] Termos MeSH primário: Histonas/metabolismo
Sequências Reguladoras de Ácido Nucleico
[Mh] Termos MeSH secundário: Animais
Metilação de DNA
Estradiol/farmacologia
Receptor alfa de Estrogênio/metabolismo
Histonas/genética
Seres Humanos
Células MCF-7
NF-kappa B/metabolismo
RNA/biossíntese
RNA Polimerase II/metabolismo
Sequências Reguladoras de Ácido Nucleico/efeitos dos fármacos
Elongação da Transcrição Genética
Iniciação da Transcrição Genética
Ubiquitinação/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Estrogen Receptor alpha); 0 (Histones); 0 (NF-kappa B); 4TI98Z838E (Estradiol); 63231-63-0 (RNA); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1080/21541264.2017.1285852


  4 / 7708 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28451632
[Au] Autor:Galvão-Ferreira P; Lipinski M; Santos F; Barco A; Costa RM
[Ad] Endereço:Champalimaud Neuroscience Programme, Fundação Champalimaud, Lisbon, 1400-038 Portugal.
[Ti] Título:Skill Learning Modulates RNA Pol II Poising at Immediate Early Genes in the Adult Striatum.
[So] Source:eNeuro;4(2), 2017 Mar-Apr.
[Is] ISSN:2373-2822
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A multilayered complexity of epigenetic and transcriptional regulatory mechanisms underlies neuronal activity-dependent gene transcription. The regulation of RNA Pol II progression along the transcription cycle, from promoter-proximal poising (with RNA Pol II paused at promoter-proximal regions, characterized by a Ser5P -rich and Ser2P -poor RPB1 CTD) to active elongation, has emerged as a major step in transcriptional regulation across several organisms, tissues, and developmental stages, including the nervous system. However, it is not known whether this mechanism is modulated by experience. We investigated the impact of learning a motor skill on RNA Pol II phosphorylation dynamics in the adult mouse striatum. We uncovered that learning modulates the striatal phosphorylation dynamics of the CTD of the RNA Pol II RPB1 subunit, leading to an increased poising index in trained mice. We found that this modulation occurs at immediate early genes (IEGs), with increased poising of RNA Pol II at both and genes but not at constitutively expressed genes. Furthermore, we confirmed that this was learning dependent, and not just regulated by context or motor activity. These experiments demonstrate a novel phenomenon of learning induced transcriptional modulation in adult brain, which may have implications for our understanding of learning, memory allocation, and consolidation.
[Mh] Termos MeSH primário: Corpo Estriado/metabolismo
Regulação da Expressão Gênica
Genes Precoces
Aprendizagem
Destreza Motora
RNA Polimerase II/metabolismo
[Mh] Termos MeSH secundário: Animais
Condicionamento Operante
Masculino
Camundongos Endogâmicos C57BL
Fosforilação
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 2.7.7.- (RNA Polymerase II); EC 2.7.7.- (Rpb1 protein, mouse)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  5 / 7708 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29339073
[Au] Autor:Roginski RS; Lau CW; Santoiemma PP; Weaver SJ; Du P; Soteropoulos P; Yang J
[Ad] Endereço:Department of Anesthesiology, CMC VA Medical Center, Philadelphia, PA 19104, United States; Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States. Electronic address: raymond.roginski@va.gov.
[Ti] Título:The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha.
[So] Source:Gene;648:42-53, 2018 Mar 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The known functions of the human GCOM1 complex hub gene include transcription elongation and the intercalated disk of cardiac myocytes. However, in all likelihood, the gene's most interesting, and thus far least understood, roles will be found in the central nervous system. To investigate the functions of the GCOM1 gene in the CNS, we have cloned human and rat brain cDNAs encoding novel, 105 kDa GCOM1 combined (Gcom) proteins, designated Gcom15, and identified a new group of GCOM1 interacting genes, termed Gints, from yeast two-hybrid (Y2H) screens. We showed that Gcom15 interacts with the NR1 subunit of the NMDA receptor by co-expression in heterologous cells, in which we observed bi-directional co-immunoprecipitation of human Gcom15 and murine NR1. Our Y2H screens revealed 27 novel GCOM1 interacting genes, many of which are synaptic proteins and/or play roles in neurologic diseases. Finally, we showed, using rat brain protein preparations, that the Gint internexin-alpha (INA), a known interactor of the NMDAR, co-IPs with GCOM1 proteins, suggesting a GCOM1-GRIN1-INA interaction and a novel pathway that may be relevant to neuroprotection.
[Mh] Termos MeSH primário: Proteínas de Filamentos Intermediários/metabolismo
RNA Polimerase II/metabolismo
Receptores de N-Metil-D-Aspartato/metabolismo
[Mh] Termos MeSH secundário: Adulto
Animais
Encéfalo/metabolismo
Células HEK293
Seres Humanos
Imunoprecipitação
Proteínas de Filamentos Intermediários/genética
Masculino
Camundongos
Proteínas do Tecido Nervoso/genética
Proteínas do Tecido Nervoso/metabolismo
Ligação Proteica
Mapas de Interação de Proteínas
Subunidades Proteicas/genética
Subunidades Proteicas/metabolismo
RNA Polimerase II/genética
Ratos Wistar
Receptores de N-Metil-D-Aspartato/genética
Técnicas do Sistema de Duplo-Híbrido
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (GRIN1 protein, human); 0 (Intermediate Filament Proteins); 0 (NR1 NMDA receptor); 0 (Nerve Tissue Proteins); 0 (POLR2M protein, human); 0 (Protein Subunits); 0 (Receptors, N-Methyl-D-Aspartate); 0 (alpha-internexin); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


  6 / 7708 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27773677
[Au] Autor:Jeronimo C; Langelier MF; Bataille AR; Pascal JM; Pugh BF; Robert F
[Ad] Endereço:Institut de recherches cliniques de Montréal, 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada.
[Ti] Título:Tail and Kinase Modules Differently Regulate Core Mediator Recruitment and Function In Vivo.
[So] Source:Mol Cell;64(3):455-466, 2016 Nov 03.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mediator is a highly conserved transcriptional coactivator organized into four modules, namely Tail, Middle, Head, and Kinase (CKM). Previous work suggests regulatory roles for Tail and CKM, but an integrated model for these activities is lacking. Here, we analyzed the genome-wide distribution of Mediator subunits in wild-type and mutant yeast cells in which RNA polymerase II promoter escape is blocked, allowing detection of transient Mediator forms. We found that although all modules are recruited to upstream activated regions (UAS), assembly of Mediator within the pre-initiation complex is accompanied by the release of CKM. Interestingly, our data show that CKM regulates Mediator-UAS interaction rather than Mediator-promoter association. In addition, although Tail is required for Mediator recruitment to UAS, Tailless Mediator nevertheless interacts with core promoters. Collectively, our data suggest that the essential function of Mediator is mediated by Head and Middle at core promoters, while Tail and CKM play regulatory roles.
[Mh] Termos MeSH primário: Regulação Fúngica da Expressão Gênica
Complexo Mediador/genética
RNA Polimerase II/genética
Proteínas de Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/genética
Fator de Transcrição TFIIB/genética
[Mh] Termos MeSH secundário: Sítios de Ligação
Complexo Mediador/metabolismo
Modelos Moleculares
Regiões Promotoras Genéticas
Ligação Proteica
Conformação Proteica em alfa-Hélice
Conformação Proteica em Folha beta
Domínios e Motivos de Interação entre Proteínas
Subunidades Proteicas/genética
Subunidades Proteicas/metabolismo
RNA Polimerase II/metabolismo
Saccharomyces cerevisiae/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Fator de Transcrição TFIIB/metabolismo
Iniciação da Transcrição Genética
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mediator Complex); 0 (Protein Subunits); 0 (SUA7 protein, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins); 0 (Transcription Factor TFIIB); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


  7 / 7708 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28468956
[Au] Autor:Jasnovidova O; Krejcikova M; Kubicek K; Stefl R
[Ad] Endereço:CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic olga.jasnovidova@ceitec.muni.cz richard.stefl@ceitec.muni.cz.
[Ti] Título:Structural insight into recognition of phosphorylated threonine-4 of RNA polymerase II C-terminal domain by Rtt103p.
[So] Source:EMBO Rep;18(6):906-913, 2017 Jun.
[Is] ISSN:1469-3178
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phosphorylation patterns of the C-terminal domain (CTD) of largest subunit of RNA polymerase II (called the CTD code) orchestrate the recruitment of RNA processing and transcription factors. Recent studies showed that not only serines and tyrosines but also threonines of the CTD can be phosphorylated with a number of functional consequences, including the interaction with yeast transcription termination factor, Rtt103p. Here, we report the solution structure of the Rtt103p CTD-interacting domain (CID) bound to Thr4 phosphorylated CTD, a poorly understood letter of the CTD code. The structure reveals a direct recognition of the phospho-Thr4 mark by Rtt103p CID and extensive interactions involving residues from three repeats of the CTD heptad. Intriguingly, Rtt103p's CID binds equally well Thr4 and Ser2 phosphorylated CTD A doubly phosphorylated CTD at Ser2 and Thr4 diminishes its binding affinity due to electrostatic repulsion. Our structural data suggest that the recruitment of a CID-containing CTD-binding factor may be coded by more than one letter of the CTD code.
[Mh] Termos MeSH primário: RNA Polimerase II/química
Proteínas de Saccharomyces cerevisiae/química
Treonina/química
Fatores de Transcrição/química
[Mh] Termos MeSH secundário: Fosforilação
Ligação Proteica
Proteínas Quinases/metabolismo
Estrutura Terciária de Proteína
Proteólise
RNA Polimerase II/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Serina/metabolismo
Treonina/metabolismo
Fatores de Transcrição/metabolismo
Transcrição Genética
Tirosina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Rtt103 protein, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins); 0 (Transcription Factors); 2ZD004190S (Threonine); 42HK56048U (Tyrosine); 452VLY9402 (Serine); EC 2.7.- (Protein Kinases); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.15252/embr.201643723


  8 / 7708 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29372961
[Au] Autor:Mazina MY; Derevyanko PK; Kocheryzhkina EV; Nikolenko YV; Krasnov AN; Vorobyeva NE
[Ti] Título:[Coactivator complexes participate in different stages of the Drosophila melanogaster hsp70 gene transcription].
[So] Source:Genetika;53(2):155-64, 2017 Feb.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The objective of this study was to identify transcriptional coactivators participating in transcription elongation of the hsp70 gene induced by heat shock. We found that all investigated coactivator complexes participate in transcription of this gene, as significant level of them were present at the gene promoter in its active state. For most of the coactivators (except for p300/CBP, Set2, and Mediator complex), we also observed a considerable increase of their binding level at the coding region of the gene after activation of its transcription by heat shock. We assume that coactivators CHD1, ISWI, Brm, Kismet-L, INO80, Mi-2, Gcn5, Lid/KDM5, Set1, DART1, DART4, SSRP1, PAF1, and Fs(1)h/Brd4 bind to the promoter of the active hsp70 gene and migrate to its coding region together with elongating RNA polymerase II. It can be suggested that some of these coactivators play an important role in stimulating the transition of the RNA polymerase II complex from transcription initiation to elongation stage.
[Mh] Termos MeSH primário: Proteínas de Drosophila/metabolismo
Proteínas de Choque Térmico HSP70/biossíntese
Complexos Multiproteicos/metabolismo
RNA Polimerase II/metabolismo
Transcrição Genética/fisiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Drosophila/genética
Drosophila melanogaster
Proteínas de Choque Térmico HSP70/genética
Complexos Multiproteicos/genética
RNA Polimerase II/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (HSP70 Heat-Shock Proteins); 0 (Multiprotein Complexes); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE


  9 / 7708 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29326042
[Au] Autor:Hatta M; Miyake Y; Uchida K; Yamazaki J
[Ad] Endereço:Department of Physiological Science and Molecular Biology, Fukuoka Dental College, Fukuoka, Japan. Electronic address: hatta@college.fdcnet.ac.jp.
[Ti] Título:Keratin 13 gene is epigenetically suppressed during transforming growth factor-ß1-induced epithelial-mesenchymal transition in a human keratinocyte cell line.
[So] Source:Biochem Biophys Res Commun;496(2):381-386, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Epithelial-mesenchymal transition (EMT) is a biological event in which epithelial cells lose their polarity and cell-cell adhesions and concomitantly acquire mesenchymal traits, and is thought to play an important role in pathological processes such as wound healing and cancer progression. In this study, we evaluated transforming growth factor (TGF)-ß1-treated human keratinocyte HaCaT cells as an in vitro model of EMT. HaCaT cells were changed into an elongated fibroblast-like morphology, which is indicative of EMT in response to TGF-ß1. Phalloidin staining demonstrated the formation of actin stress fibers in TGF-ß1-treated cells. Quantitative RT-PCR analysis revealed that TGF-ß1 increased the mRNA levels of EMT transcription factors (SNAI2, TWIST1, and ZEB1) and mesenchymal markers (CDH2, VIM, and FN1), while it decreased the transcripts of epithelial phenotypic genes (CLDN1, OCLN, KRT5, KRT15, KRT13, and TGM1). Furthermore, we found that KRT13 was drastically suppressed through the reduction of RNA polymerase II occupancy of its promoter, which was accompanied by a decrease in active histone marks (H3K4me3 and H3K27ac) and an increase in a repressive mark (H3K27me3) during EMT. These findings indicate that the TGF-ß1-induced EMT program regulates a subset of epithelial and mesenchymal marker genes, and that KRT13 is transcriptionally suppressed through the modulation of the chromatin state at the KRT13 promoter in HaCaT cells.
[Mh] Termos MeSH primário: Epigênese Genética
Transição Epitelial-Mesenquimal/efeitos dos fármacos
Queratina-13/genética
Queratinócitos/efeitos dos fármacos
Fator de Crescimento Transformador beta1/farmacologia
[Mh] Termos MeSH secundário: Actinas/genética
Actinas/metabolismo
Antígenos CD/genética
Antígenos CD/metabolismo
Caderinas/genética
Caderinas/metabolismo
Diferenciação Celular/efeitos dos fármacos
Linhagem Celular Transformada
Citocinas/genética
Citocinas/metabolismo
Transição Epitelial-Mesenquimal/genética
Fibroblastos/citologia
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Histonas/genética
Histonas/metabolismo
Seres Humanos
Queratina-13/metabolismo
Queratinócitos/citologia
Queratinócitos/metabolismo
Proteínas Nucleares/genética
Proteínas Nucleares/metabolismo
Regiões Promotoras Genéticas
RNA Polimerase II/genética
RNA Polimerase II/metabolismo
Transdução de Sinais
Fatores de Transcrição da Família Snail/genética
Fatores de Transcrição da Família Snail/metabolismo
Proteína 1 Relacionada a Twist/genética
Proteína 1 Relacionada a Twist/metabolismo
Homeobox 1 de Ligação a E-box em Dedo de Zinco/genética
Homeobox 1 de Ligação a E-box em Dedo de Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins); 0 (Antigens, CD); 0 (CDH2 protein, human); 0 (Cadherins); 0 (Cytokines); 0 (Histones); 0 (KRT13 protein, human); 0 (Keratin-13); 0 (Nuclear Proteins); 0 (SNAI2 protein, human); 0 (Snail Family Transcription Factors); 0 (TGFB1 protein, human); 0 (TWIST1 protein, human); 0 (Transforming Growth Factor beta1); 0 (Twist-Related Protein 1); 0 (ZEB1 protein, human); 0 (Zinc Finger E-box-Binding Homeobox 1); 0 (migration stimulating factor, human); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


  10 / 7708 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28456963
[Au] Autor:Bogolyubova IO; Bogolyubov DS
[Ad] Endereço:Institute of Cytology RAS, 4 Tikhoretsky Ave., St. Petersburg, 194064, Russia. ibogol@mail.ru.
[Ti] Título:Detection of RNA Polymerase II in Mouse Embryos During Zygotic Genome Activation Using Immunocytochemistry.
[So] Source:Methods Mol Biol;1605:147-159, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mammalian pre-implantation embryos represent a highly dynamic experimental model for comparative studies of nuclear structure and functions in the context of gradual reactivation of transcription. Here, we present details of the methods that allow localizing RNA polymerase II in mouse pre-implantation embryos with specific antibodies, using fluorescent/confocal and electron microscopy. We stress the special aspects of immunolabeling protocols in respect to the embryonic material. We made a special emphasis on the essential steps preceding the immunocytochemical experiments. In particular, we consider the procedures of female hormonal stimulation and embryo collection. The described approaches are also applicable to study other nuclear proteins.
[Mh] Termos MeSH primário: Embrião de Mamíferos/metabolismo
Imuno-Histoquímica/métodos
RNA Polimerase II/análise
[Mh] Termos MeSH secundário: Animais
Camundongos
Fixação de Tecidos
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6988-3_10



página 1 de 771 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde