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Pesquisa : D08.811.913.696.620.275 [Categoria DeCS]
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[PMID]:28759390
[Au] Autor:Montoya-Williams D; Mowitz M
[Ad] Endereço:Division of Neonatology, Department of Pediatrics, University of Florida, Gainesville, Florida dmontoyafontalvo@peds.ufl.edu.
[Ti] Título:Cholestasis and Hepatic Iron Deposition in an Infant With Complex Glycerol Kinase Deficiency.
[So] Source:Pediatrics;140(1), 2017 Jul.
[Is] ISSN:1098-4275
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We present a 6-week-old male infant with persistent hyperbilirubinemia, hypertriglyceridemia, elevated creatine kinase levels, and transaminitis since the second week of life. When he developed hyperkalemia, clinical suspicion was raised for adrenal insufficiency despite hemodynamic stability. A full endocrine workup revealed nearly absent adrenocorticotropic hormone. Coupled with his persistent hypertriglyceridemia (peak of 811 mg/dL) and elevated creatine kinase levels (>20 000 U/L), his corticotropin level lead to a clinical diagnosis of complex glycerol kinase deficiency (GKD), also known as Xp21 deletion syndrome. This complex disorder encompasses the phenotype of Duchenne muscular dystrophy, GKD, and congenital adrenal hypoplasia due to the deletion of 3 contiguous genetic loci on the X chromosome. Our case exemplifies the presentation of this disorder and highlights the important lesson of distinguishing between adrenal hypoplasia congenita and congenital adrenal hyperplasia, as well as the sometimes subtle presentation of adrenal insufficiency. To our knowledge, it is also the first reported case of complex GKD deficiency with the additional finding of hepatic iron deposition, which may indicate a potential area for exploration regarding the pathogenesis of liver injury and cholestasis seen in cortisol-related endocrinopathies.
[Mh] Termos MeSH primário: Erros Inatos do Metabolismo dos Carboidratos/diagnóstico
Glucocorticoides/uso terapêutico
Glicerol Quinase/deficiência
[Mh] Termos MeSH secundário: Hormônio Adrenocorticotrópico/sangue
Erros Inatos do Metabolismo dos Carboidratos/tratamento farmacológico
Colestase/etiologia
Creatina Quinase/sangue
Diagnóstico Diferencial
Seres Humanos
Hipertrigliceridemia/etiologia
Hipoadrenocorticismo Familiar
Lactente
Ferro/metabolismo
Fígado/patologia
Masculino
Análise em Microsséries
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucocorticoids); 9002-60-2 (Adrenocorticotropic Hormone); E1UOL152H7 (Iron); EC 2.7.1.30 (Glycerol Kinase); EC 2.7.3.2 (Creatine Kinase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE


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[PMID]:28358426
[Au] Autor:Tomizawa M; Shinozaki F; Motoyoshi Y; Sugiyama T; Yamamoto S; Ishige N
[Ad] Endereço:Department of Gastroenterology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284­0003, Japan.
[Ti] Título:2­Deoxy­D­glucose initiates hepatocyte differentiation in human induced pluripotent stem cells.
[So] Source:Mol Med Rep;15(5):3083-3087, 2017 May.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:To initiate hepatocyte differentiation in human induced pluripotent stem (iPS) cells, cells are cultured in a medium lacking glucose but supplemented with galactose (hepatocyte selection medium, HSM) or in medium supplemented with oncostatin M and small molecules (hepatocyte differentiation inducer, HDI). In the present study, 2­Deoxy­D­glucose (2DG), an analogue of glucose, was utilized instead of glucose deprivation and the effect of 2DG supplementation on iPS differentiation was examined. First, 201B7 cells, an iPS cell line, were cultured in HSM or HDI media for 2 days and then subjected to reverse transcription­quantitative polymerase chain reaction (RT­qPCR) in order to analyze expression levels of established hepatocyte markers, including cytosolic aspartate aminotransferase (AST), mitochondrial AST, alanine aminotransferase (ALT), and glycerol kinase. mRNA expression levels of mitochondrial AST, ALT, and glycogen synthase significantly increased following culture in HSM and HDI compared with ReproFF media. Cytosolic AST mRNA expression levels significantly increased following culture in HDI compared with ReproFF media, but not in HSM. To test the effect of 2DG on iPS differentiation, 201B7 cells were cultured in ReproFF, a feeder­free medium that retains pluripotency, supplemented with 2DG. Following 7 days of culture, the cells were subjected to RT­qPCR to analyze expression levels of α­fetoprotein (AFP), a marker of immature hepatocytes. AFP mRNA expression levels significantly increased with the addition of 0.1 µM 2DG in the media, and galactose addition acted synergistically with 2DG to further upregulate AFP expression. In conclusion, the present study demonstrated that hepatocyte differentiation was initiated in iPS cells cultured in HSM and HDI media and that 2DG could be used as a supplement instead of glucose deprivation to initiate hepatocyte differentiation in iPS cells.
[Mh] Termos MeSH primário: Diferenciação Celular/efeitos dos fármacos
Desoxiglucose/farmacologia
Hepatócitos/citologia
Hepatócitos/efeitos dos fármacos
Células-Tronco Pluripotentes Induzidas/citologia
Células-Tronco Pluripotentes Induzidas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Alanina Transaminase/metabolismo
Aspartato Aminotransferase Citoplasmática/metabolismo
Aspartato Aminotransferase Mitocondrial/metabolismo
Linhagem Celular
Galactose/metabolismo
Glucose/metabolismo
Glicerol Quinase/metabolismo
Glicogênio Sintase/metabolismo
Hepatócitos/metabolismo
Seres Humanos
Células-Tronco Pluripotentes Induzidas/metabolismo
Oncostatina M/farmacologia
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase em Tempo Real/métodos
alfa-Fetoproteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (alpha-Fetoproteins); 106956-32-5 (Oncostatin M); 9G2MP84A8W (Deoxyglucose); EC 2.4.1.11 (Glycogen Synthase); EC 2.6.1.- (Aspartate Aminotransferase, Cytoplasmic); EC 2.6.1.- (Aspartate Aminotransferase, Mitochondrial); EC 2.6.1.2 (Alanine Transaminase); EC 2.7.1.30 (Glycerol Kinase); IY9XDZ35W2 (Glucose); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2017.6405


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[PMID]:28284306
[Au] Autor:Narwal V; Pundir CS
[Ad] Endereço:Department of Biochemistry, M.D. University, Rohtak, Haryana, India.
[Ti] Título:An improved amperometric triglyceride biosensor based on co-immobilization of nanoparticles of lipase, glycerol kinase and glycerol 3-phosphate oxidase onto pencil graphite electrode.
[So] Source:Enzyme Microb Technol;100:11-16, 2017 May.
[Is] ISSN:1879-0909
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nanoparticles (NPs) of commercial lipase from Candida rugosa, of glycerol kinase (GK) from Cellulomonas species, of glycerol-3- phosphate oxidase (GPO) from Aerococcus viridans were prepared, characterized and co-immobilized onto a pencil graphite (PG) electrode. The morphological and electrochemical characterization of PG electrode was performed by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) before and after co-immobilization of enzyme nanoparticles (ENPs). An improved amperometric triglyceride (TG) biosensor was fabricated using Lipase NPs/GKNPs/GPONPs/PG electrode as the working electrode, Ag/AgCl as the standard electrode and Pt wire as auxiliary electrode. The biosensor showed optimum response within 2.5s at a pH 7.0 and temperature of 35°C. The biosensor measured current due to electrons generated at 0.1V against Ag/AgCl, from H O , which is produced from triolein by co-immobilized ENPs. A linear relationship was obtained over between a wide triolein concentration range (0.1mM-45mM) and current (mA) under optimal conditions. The Lipase NPs/GKNPs/GPONPs/PG electrode showed high sensitivity (1241±20mAcm mM ); a lower detection limit (0.1nM) and good correlation coeficient (R =0.99) with a standard enzymic colorimetric method. Analytical recovery of added triolein in serum was 98.01%, within and between batch coefficients of variation (CV) were 0.05% and 0.06% respectively. The biosensor was evaluated and employed for determination of TG in the serum of apparently healthy subject and persons suffering from hypertriglyceridemia. The biosensor lost 20% of its initial activity after its continued uses over a period of 240days, while being stored at 4°C.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Triglicerídeos/análise
[Mh] Termos MeSH secundário: Adulto
Análise Química do Sangue/métodos
Estudos de Casos e Controles
Espectroscopia Dielétrica
Estabilidade Enzimática
Enzimas Imobilizadas
Feminino
Glicerol Quinase
Glicerolfosfato Desidrogenase
Grafite
Seres Humanos
Hipertrigliceridemia/sangue
Lipase
Masculino
Microscopia Eletrônica
Nanopartículas/ultraestrutura
Triglicerídeos/sangue
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzymes, Immobilized); 0 (Triglycerides); 7782-42-5 (Graphite); EC 1.1.- (Glycerolphosphate Dehydrogenase); EC 1.1.3.21 (glycerol-3-phosphate oxidase); EC 2.7.1.30 (Glycerol Kinase); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


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[PMID]:28058519
[Au] Autor:Lei C; Tian J; Ji H
[Ad] Endereço:College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China.
[Ti] Título:Stimulation of glycerol kinase in grass carp preadipocytes by EPA.
[So] Source:Fish Physiol Biochem;43(3):813-822, 2017 Jun.
[Is] ISSN:1573-5168
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study was conducted to assess the effect of eicosapentaenoic acid (EPA) on grass carp preadipocyte glycerol kinase (GyK) expression, as well as to explore the mechanism. Here, we cloned partial sequence of grass carp GyK gene and analyzed its tissue distribution. The result showed that GyK gene expressed most in the liver, followed by adipose tissue and the kidney. Besides, 400 µM oleic acid (18:1n-9, OA) was used to establish a hypertrophic preadipocyte model. GyK gene expression and enzyme activity were significantly enhanced after model cells were treated with 100 µM eicosapentaenoic acid (20:5n-3, EPA) for 6, 12, and 24 h. Meanwhile, peroxisome proliferative-activated receptor (PPAR)γ, adipose triglyceride lipase (ATGL), and the two isoforms of grass carp HSL gene were first identified by Sun et al (2016), and they defined the two isoforms as HSLa and HSLb. Therefore, maybe HSLa and HSLb are appropriate.. The content of triglyceride was dramatically increased by EPA treatment for 24 h. Further, a competitive ATGL antagonist, HY-15859, attenuated the increase in GyK induced by EPA at 12 h. Surprisingly, the enhanced lipolysis and PPARγ gene expression induced by serum deprivation were paralleled by an increase in GyK gene expression, whereas a stabilization in GyK enzyme activity. Other fatty acids, including docosahexaenoic acid, alpha-linolenic acid, linoleic acid, and OA also promoted GyK gene expression. Moreover, an irreversible PPARγ antagonist, GW9662, was used to investigate the role of PPARγ in GyK induction. Data showed that GW9662 abolished the induction of GyK by EPA at 12 h. Together, these data suggested that EPA elevated grass carp preadipocytes GyK expression. ATGL and PPARγ contributed to the induction of GyK. PPARγ may be a key regulator in response to GyK expression induced by EPA.
[Mh] Termos MeSH primário: Adipócitos/fisiologia
Carpas
Ácido Eicosapentaenoico/farmacologia
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Glicerol Quinase/metabolismo
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Glicerol Quinase/genética
Lipólise/efeitos dos fármacos
Lipólise/fisiologia
PPAR gama/genética
PPAR gama/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (PPAR gamma); AAN7QOV9EA (Eicosapentaenoic Acid); EC 2.7.1.30 (Glycerol Kinase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1007/s10695-016-0336-x


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[PMID]:27992107
[Au] Autor:Molla GS; Kinfu BM; Chow J; Streit W; Wohlgemuth R; Liese A
[Ad] Endereço:Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany.
[Ti] Título:Bioreaction Engineering Leading to Efficient Synthesis of L-Glyceraldehyd-3-Phosphate.
[So] Source:Biotechnol J;12(3), 2017 Mar.
[Is] ISSN:1860-7314
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Enantiopure L-glyceraldehyde-3-phosphate (L-GAP) is a useful building block in natural biological and synthetic processes. A biocatalytic process using glycerol kinase from Cellulomonas sp. (EC 2.7.1.30) catalyzed phosphorylation of L-glyceraldehyde (L-GA) by ATP is used for the synthesis of L-GAP. L-GAP has a half-life of 6.86 h under reaction conditions. The activity of this enzyme depends on the Mg to ATP molar ratio showing maximum activity at the optimum molar ratio of 0.7. A kinetic model is developed and validated showing a 2D correlation of 99.9% between experimental and numerical data matrices. The enzyme exhibits inhibition by ADP, AMP, methylglyoxal and Ca , but not by L-GAP and inorganic orthophosphate. Moreover, equal amount of Ca exerts a different degree of inhibition relative to the activity without the addition of Ca depending on the Mg to ATP molar ratio. If the Mg to ATP molar ratio is set to be at the optimum value or less, inorganic hexametaphosphate (PPi6) suppresses the enzyme activity; otherwise PPi6 enhances the enzyme activity. Based on reaction engineering parameters such as conversion, selectivity and specific productivity, evaluation of different reactor types reveals that batchwise operation via stirred-tank reactor is the most efficient process for the synthesis of L-GAP.
[Mh] Termos MeSH primário: Bioengenharia
Gliceraldeído 3-Fosfato/biossíntese
Glicerol Quinase/metabolismo
[Mh] Termos MeSH secundário: Cellulomonas/enzimologia
Escherichia coli/enzimologia
Meia-Vida
Fosfatos/metabolismo
Reprodutibilidade dos Testes
Streptomyces/enzimologia
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phosphates); 13478-98-3 (hexametaphosphate); 142-10-9 (Glyceraldehyde 3-Phosphate); EC 2.7.1.30 (Glycerol Kinase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161220
[St] Status:MEDLINE
[do] DOI:10.1002/biot.201600625


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[PMID]:27876382
[Au] Autor:Pundir CS; Aggarwal V
[Ad] Endereço:Department of Biochemistry, M.D. University, Rohtak 124001, Haryana, India. Electronic address: chandraspundir@gmail.com.
[Ti] Título:Amperometric triglyceride bionanosensor based on nanoparticles of lipase, glycerol kinase, glycerol-3-phosphate oxidase.
[So] Source:Anal Biochem;517:56-63, 2017 Jan 15.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The nanoparticles (NPs) aggregates of lipase from porcine pancreas, glycerol kinase (GK) from Cellulomonas sp. and glycerol-3-phosphate oxidase (GPO) from Aerococcus viridanss were prepared by desolvation and glutaraldehyde crosslinking and functionalized by cysteamine. These enzyme nanoparticles (ENPs) were characterized by transmission electron microscopy (TEM) and Fourier transform infra red (FTIR) spectroscopy. The functionalzed ENPs aggregates were co-immobilized covalently onto polycrystalline Au electrode through thiolated bond. An improved amperometric triglyceride (TG) bionanosensor was constructed using this ENPs modified Au electrode as working electrode. Biosensor showed optimum current at 1.2 V within 5s, at pH 6.5 and 35 °C.A linear relationship was obtained between current (mA) and triolein concentration in lower concentration range,10-100 mg/dL and higher concentration range, 100-500 mg/dL. Limit of detection (LOD) of bionanosensor was 1.0 µg/ml. Percent analytical recovery of added trolein (50 and 100 mg/dL) in serum was 95.2 ± 0.5 and 96.0 ± 0.17. Within and between batch coefficients of variation (CV) were 2.33% and 2.15% respectively. A good correlation (R = 0.99) was obtained between TG values in sera measured by present biosensor and standard enzymic colorimetric method with the regression equation: y= (0.993x + 0.967). ENPs/Au electrode was used 180 times over a period of 3 months with 50% loss in its initial activity, when stored dry at 4 °C.
[Mh] Termos MeSH primário: Aerococcus/enzimologia
Proteínas de Bactérias/química
Técnicas Biossensoriais/métodos
Cellulomonas/enzimologia
Glicerol Quinase/química
Glicerolfosfato Desidrogenase/química
Lipase/química
Nanopartículas/química
Triglicerídeos/sangue
[Mh] Termos MeSH secundário: Animais
Feminino
Seres Humanos
Masculino
Nanopartículas/ultraestrutura
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Triglycerides); EC 1.1.- (Glycerolphosphate Dehydrogenase); EC 1.1.3.21 (glycerol-3-phosphate oxidase); EC 2.7.1.30 (Glycerol Kinase); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE


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[PMID]:27769297
[Au] Autor:Shen W; Xue Y; Liu Y; Kong C; Wang X; Huang M; Cai M; Zhou X; Zhang Y; Zhou M
[Ad] Endereço:State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
[Ti] Título:A novel methanol-free Pichia pastoris system for recombinant protein expression.
[So] Source:Microb Cell Fact;15(1):178, 2016 Oct 21.
[Is] ISSN:1475-2859
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: As one of the most popular expression systems, recombinant protein expression in Pichia pastoris relies on the AOX1 promoter (P ) which is strongly induced by methanol. However, the toxic and inflammatory nature of methanol restricts its application, especially in edible and medical products. Therefore, constructing a novel methanol-free system becomes necessary. The kinases involved in P activation or repression by different carbon sources may be promising targets. RESULTS: We identified two kinase mutants: Δgut1 and Δdak, both of which showed strong alcohol oxidase activity under non-methanol carbon sources. Based on these two kinases, we constructed two methanol-free expression systems: Δgut1-HpGCY1-glycerol (P induced by glycerol) and Δdak-DHA (P induced by DHA). By comparing their GFP expression efficiencies, the latter one showed better potential. To further test the Δdak-DHA system, three more recombinant proteins were expressed as examples. We found that the expression ability of our novel methanol-free Δdak-DHA system was generally better than the constitutive GAP promoter, and reached 50-60 % of the traditional methanol induced system. CONCLUSIONS: We successfully constructed a novel methanol-free expression system Δdak-DHA. This modified expression platform preserved the favorable regulatable nature of P , providing a potential alternative to the traditional system.
[Mh] Termos MeSH primário: Pichia/genética
Pichia/metabolismo
Proteínas Recombinantes/biossíntese
[Mh] Termos MeSH secundário: Oxirredutases do Álcool/genética
Oxirredutases do Álcool/metabolismo
Di-Hidroxiacetona/metabolismo
Di-Hidroxiacetona/farmacologia
Técnicas de Inativação de Genes
Glicerol/metabolismo
Glicerol/farmacologia
Glicerol Quinase/genética
Glicerol Quinase/metabolismo
Metanol/farmacologia
Proteínas Recombinantes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.3.13 (alcohol oxidase); EC 2.7.1.30 (Glycerol Kinase); O10DDW6JOO (Dihydroxyacetone); PDC6A3C0OX (Glycerol); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


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[PMID]:27724997
[Au] Autor:Kubota M; Watanabe R; Yamaguchi M; Hosojima M; Saito A; Fujii M; Fujimura S; Kadowaki M
[Ad] Endereço:1Center for Transdisciplinary Research,Niigata University,8050,Ikarashi 2-no-cho,Nishi-ku,Niigata 950-2181,Japan.
[Ti] Título:Rice endosperm protein slows progression of fatty liver and diabetic nephropathy in Zucker diabetic fatty rats.
[So] Source:Br J Nutr;116(8):1326-1335, 2016 Oct.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We previously reported that rice endosperm protein (REP) has renoprotective effects in Goto-Kakizaki rats, a non-obese diabetic model. However, whether these effects occur in obese diabetes remains unclear. This study aimed to clarify the effects of REP on obese diabetes, especially on fatty liver and diabetic nephropathy, using the obese diabetic model Zucker diabetic fatty (ZDF) rats. In total, 7-week-old male ZDF rats were fed diets containing 20 % REP or casein (C) for 8 weeks. Changes in fasting blood glucose levels and urinary markers were monitored during the experimental period. Hepatic lipids and metabolites were measured and renal glomeruli were observed morphologically. HbA1c levels were significantly lower in rats fed REP, compared with C (P<0·05). Compared with C in the liver, REP prevented lipid accumulation (total lipid, TAG and total cholesterol, P<0·01). Liver metabolome analysis indicated that levels of metabolites associated with glycolysis, the pentose phosphate pathway and carnitine metabolism were significantly greater in the REP group than in the C group (P<0·05), suggesting activation of both glucose catabolism and fatty acid oxidation. The metabolite increases promoted by REP may contribute to suppression of liver lipid accumulation. Urinary excretion of albumin and N-acetyl-ß-d-glucosaminidase was significantly reduced in rats fed REP for 8 weeks (P<0·01). In addition, there was a distinct suppression of mesangial matrix expansion and glomerular hypertrophy in response to REP (P<0·01). Thus, REP had preventive effects on obese diabetes, fatty liver and diabetic nephropathy.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 2/dietoterapia
Nefropatias Diabéticas/dietoterapia
Dieta Vegetariana
Endosperma/química
Hepatopatia Gordurosa não Alcoólica/dietoterapia
Oryza/química
Proteínas de Vegetais Comestíveis/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Biomarcadores/sangue
Biomarcadores/metabolismo
Biomarcadores/urina
Erros Inatos do Metabolismo dos Carboidratos/prevenção & controle
Diabetes Mellitus Tipo 2/complicações
Diabetes Mellitus Tipo 2/metabolismo
Diabetes Mellitus Tipo 2/patologia
Nefropatias Diabéticas/complicações
Nefropatias Diabéticas/metabolismo
Nefropatias Diabéticas/fisiopatologia
Dieta Vegetariana/efeitos adversos
Progressão da Doença
Metabolismo Energético
Glicerol Quinase/deficiência
Hiperfosfatemia/etiologia
Hiperfosfatemia/prevenção & controle
Hipoadrenocorticismo Familiar
Glomérulos Renais/patologia
Glomérulos Renais/fisiopatologia
Metabolismo dos Lipídeos
Fígado/metabolismo
Fígado/patologia
Masculino
Hepatopatia Gordurosa não Alcoólica/complicações
Hepatopatia Gordurosa não Alcoólica/metabolismo
Hepatopatia Gordurosa não Alcoólica/fisiopatologia
Obesidade/complicações
Tamanho do Órgão
Ratos Zucker
Insuficiência Renal Crônica/etiologia
Insuficiência Renal Crônica/prevenção & controle
Proteínas de Vegetais Comestíveis/efeitos adversos
Ganho de Peso
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Vegetable Proteins); EC 2.7.1.30 (Glycerol Kinase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161012
[St] Status:MEDLINE


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[PMID]:27713003
[Au] Autor:So V; Jalan D; Lemaire M; Topham MK; Hatch GM; Epand RM
[Ad] Endereço:Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8S 4K1, Canada.
[Ti] Título:Diacylglycerol kinase epsilon suppresses expression of p53 and glycerol kinase in mouse embryo fibroblasts.
[So] Source:Biochim Biophys Acta;1861(12 Pt A):1993-1999, 2016 12.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The incorporation of glycerol into lipid was measured using SV40 transformed mouse embryo fibroblasts (MEFs) from either wild-type (WT) mice or from mice in which the epsilon isoform of diacylglycerol kinase (DGKε) was knocked out (DGKε ). We present an explanation for our finding that DGKε MEFs exhibited greater uptake of H-glycerol into the cell and a greater incorporation into lipids compared with their WT counterparts, with no change in the relative amounts of various lipids between the DGKε and WT MEFs. Glycerol kinase is more highly expressed in the DGKε cells than in their WT counterparts. In addition, the activity of glycerol kinase is greater in the DGKε cells than in their WT counterparts. Other substrates that enter the cell independent of glycerol kinase, such as pyruvate or acetate, are incorporated into lipid to the same extent between DGKε and WT cell lines. We also show that expression of p53, a transcription factor that increases the synthesis of glycerol kinase, is increased in DGKε MEFs in comparison to WT cells. We conclude that the increased incorporation of glycerol into lipids in DGKε cells is a consequence of up-regulation of glycerol kinase and not a result of an increase in the rate of lipid synthesis. Furthermore, increased expression of the pro-survival gene, p53, in cells knocked out for DGKε suggests that cells over-expressing DGKε would have a greater propensity to become tumorigenic.
[Mh] Termos MeSH primário: Diacilglicerol Quinase/metabolismo
Fibroblastos/metabolismo
Glicerol Quinase/metabolismo
Glicerol/metabolismo
Proteína Supressora de Tumor p53/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Lipídeos/fisiologia
Lipogênese/fisiologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Fatores de Transcrição/metabolismo
Regulação para Cima/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lipids); 0 (Transcription Factors); 0 (Tumor Suppressor Protein p53); EC 2.7.1.107 (Diacylglycerol Kinase); EC 2.7.1.30 (Glycerol Kinase); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161008
[St] Status:MEDLINE


  10 / 604 MEDLINE  
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[PMID]:27495223
[Au] Autor:Zhuo S; Yang M; Zhao Y; Chen X; Zhang F; Li N; Yao P; Zhu T; Mei H; Wang S; Li Y; Chen S; Le Y
[Ad] Endereço:Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China.
[Ti] Título:MicroRNA-451 Negatively Regulates Hepatic Glucose Production and Glucose Homeostasis by Targeting Glycerol Kinase-Mediated Gluconeogenesis.
[So] Source:Diabetes;65(11):3276-3288, 2016 Nov.
[Is] ISSN:1939-327X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:MicroRNAs (miRNAs) are a new class of regulatory molecules implicated in type 2 diabetes, which is characterized by insulin resistance and hepatic glucose overproduction. We show that miRNA-451 (miR-451) is elevated in the liver tissues of dietary and genetic mouse models of diabetes. Through an adenovirus-mediated gain- and loss-of-function study, we found that miR-451 negatively regulates hepatic gluconeogenesis and blood glucose levels in normal mice and identified glycerol kinase (Gyk) as a direct target of miR-451. We demonstrate that miR-451 and Gyk regulate hepatic glucose production, the glycerol gluconeogenesis axis, and the AKT-FOXO1-PEPCK/G6Pase pathway in an opposite manner; Gyk could reverse the effect of miR-451 on hepatic gluconeogenesis and AKT-FOXO1-PEPCK/G6Pase pathway. Moreover, overexpression of miR-451 or knockdown of Gyk in diabetic mice significantly inhibited hepatic gluconeogenesis, alleviated hyperglycemia, and improved glucose tolerance. Further studies showed that miR-451 is upregulated by glucose and insulin in hepatocytes; the elevation of hepatic miR-451 in diabetic mice may contribute to inhibiting Gyk expression. This study provides the first evidence that miR-451 and Gyk regulate the AKT-FOXO1-PEPCK/G6Pase pathway and play critical roles in hepatic gluconeogenesis and glucose homeostasis and identifies miR-451 and Gyk as potential therapeutic targets against hyperglycemia in diabetes.
[Mh] Termos MeSH primário: Diabetes Mellitus Experimental/metabolismo
Gluconeogênese/fisiologia
Glucose/metabolismo
Glicerol Quinase/metabolismo
Fígado/metabolismo
MicroRNAs/metabolismo
[Mh] Termos MeSH secundário: Animais
Diabetes Mellitus Experimental/genética
Proteína Forkhead Box O1/genética
Proteína Forkhead Box O1/metabolismo
Gluconeogênese/genética
Glucose-6-Fosfatase/genética
Glucose-6-Fosfatase/metabolismo
Glicerol Quinase/genética
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Proteína Oncogênica v-akt/genética
Proteína Oncogênica v-akt/metabolismo
Fosfoenolpiruvato Carboxiquinase (ATP)/genética
Fosfoenolpiruvato Carboxiquinase (ATP)/metabolismo
Ratos
Transdução de Sinais/genética
Transdução de Sinais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Forkhead Box Protein O1); 0 (MicroRNAs); 0 (Mirn451 microRNA, mouse); EC 2.7.1.30 (Glycerol Kinase); EC 2.7.11.1 (Oncogene Protein v-akt); EC 3.1.3.9 (Glucose-6-Phosphatase); EC 4.1.1.49 (Phosphoenolpyruvate Carboxykinase (ATP)); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160807
[St] Status:MEDLINE



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