Base de dados : MEDLINE
Pesquisa : D08.811.913.696.620.682.700.606 [Categoria DeCS]
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[PMID]:29049346
[Au] Autor:Liu P; Sharrock RA
[Ad] Endereço:Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, Montana, United States of America.
[Ti] Título:Biological activity and dimerization state of modified phytochrome A proteins.
[So] Source:PLoS One;12(10):e0186468, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To assess potential physical interactions of type I phyA with the type II phyB-phyE phytochromes in vivo, transgenes expressing fusion gene forms of phyA were introduced into the Arabidopsis phyA mutant background. When a single c-Myc (myc) epitope is added to either the N- or C-terminus of phyA, the constructs completely complement phyA mutant phenotypes. However, addition of larger tags, such as six consecutive myc epitopes or the yellow fluorescent protein sequence, result in fusion proteins that show reduced activity. All the tagged phyA proteins migrate as dimers on native gels and co-immunoprecipitation reveals no binding interaction of phyA to any of the type II phys in the dark or under continuous far-red light. Dimers of the phyA 1-615 amino acid N-terminal photosensory domain (NphyA), generated in vivo with a yeast GAL4 dimerization domain and attached to a constitutive nuclear localization sequence, are expressed at a low level and, although they cause a cop phenotype in darkness and mediate a very low fluence response to pulses of FR, have no activity under continuous FR. It is concluded that type I phyA in its Pr form is present in plants predominantly or exclusively as a homodimer and does not stably interact with type II phys in a dimer-to-dimer manner. In addition, its activity in mediating response to continuous FR is sensitive to modification of its N- or C-terminus.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Fitocromo A/metabolismo
[Mh] Termos MeSH secundário: Dimerização
Fitocromo A/química
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Phytochrome A)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171020
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186468


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[PMID]:28614407
[Au] Autor:Miao Q; Deng P; Saha S; Jenkins JN; Hsu CY; Abdurakhmonov IY; Buriev ZT; Pepper A; Ma DP
[Ad] Endereço:Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, United States of America.
[Ti] Título:Genome-wide identification and characterization of microRNAs differentially expressed in fibers in a cotton phytochrome A1 RNAi line.
[So] Source:PLoS One;12(6):e0179381, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cotton fiber is an important commodity throughout the world. Fiber property determines fiber quality and commercial values. Previous studies showed that silencing phytochrome A1 gene (PHYA1) by RNA interference in Upland cotton (Gossypium hirsutum L. cv. Coker 312) had generated PHYA1 RNAi lines with simultaneous improvements in fiber quality (longer, stronger and finer fiber) and other key agronomic traits. Characterization of the altered molecular processes in these RNAi genotypes and its wild-type controls is a great interest to better understand the PHYA1 RNAi phenotypes. In this study, a total of 77 conserved miRNAs belonging to 61 families were examined in a PHYA1 RNAi line and its parental Coker 312 genotype by using multiplex sequencing. Of these miRNAs, seven (miR7503, miR7514, miR399c, miR399d, miR160, miR169b, and miR2950) were found to be differentially expressed in PHYA1 RNAi cotton. The target genes of these differentially expressed miRNAs were involved in the metabolism and signaling pathways of phytohormones, which included Gibberellin, Auxin and Abscisic Acid. The expression of several MYB transcription factors was also affected by miRNAs in RNAi cotton. In addition, 35 novel miRNAs (novel miR1-novel miR35) were identified in fibers for the first time in this study. Target genes of vast majority of these novel miRNAs were also predicted. Of these, nine novel miRNAs (novel-miR1, 2, 16, 19, 26, 27, 28, 31 and 32) were targeted to cytochrome P450-like TATA box binding protein (TBP). The qRT-PCR confirmed expression levels of several differentially regulated miRNAs. Expression patterns of four miRNAs-targets pairs were also examined via RNA deep sequencing. Together, the results imply that the regulation of miRNA expression might confer to the phenotype of the PHYA1 RNAi line(s) with improved fiber quality.
[Mh] Termos MeSH primário: Fibra de Algodão
Regulação da Expressão Gênica de Plantas
Gossypium/genética
MicroRNAs/genética
Fitocromo A/genética
Interferência de RNA
[Mh] Termos MeSH secundário: Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Perfilação da Expressão Gênica/métodos
Genoma de Planta/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas
RNA de Plantas/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Transdução de Sinais/genética
Proteína de Ligação a TATA-Box/genética
Proteína de Ligação a TATA-Box/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (Phytochrome A); 0 (Plant Proteins); 0 (RNA, Plant); 0 (TATA-Box Binding Protein); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179381


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[PMID]:28273503
[Au] Autor:Khudyakova AY; Kreslavski VD; Shirshikova GN; Zharmukhamedov SK; Kosobryukhov AA; Allakhverdiev SI
[Ad] Endereço:Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region 142290, Russia; Institute for Biological Instrumentation, Russian Academy of Sciences, Institutskaya Street 7, Pushchino, Moscow Region 142290, Russia; Controlled Photobiosynthesis L
[Ti] Título:Resistance of Arabidopsis thaliana L. photosynthetic apparatus to UV-B is reduced by deficit of phytochromes B and A.
[So] Source:J Photochem Photobiol B;169:41-46, 2017 Apr.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The photosynthetic responses of 25-day-old Arabidopsis phyA phyB double mutant (DM) compared with the wild type (WT) to UV-B radiation (1Wm , 30min) were investigated. UV-B irradiation led to reduction of photosystem 2 (PS-2) activity and the photosynthetic rate. In plants grown under both white and red light (λ - 660nm) the reduction was greater in DM plants compared to the WT. Without UV-B irradiation a decrease in PS-2 activity was observed in DM grown under RL only. It is assumed that the lower content of UV-absorbing pigments and carotenoids observed in DM may be one of the reasons of reduced PS-2 resistance to UV-B. Higher decrease in activities under UV in DM plants grown under RL compared to DM plants grown under white light is likely due to the lack of activity of cryptochromes in plants grown under red light. Rates of post-stress recovery of photosynthetic activity of DM compared with WT plants under white and red light of low intensity were studied. Almost complete recovery of the activity was found which was not observed under dark conditions and in the presence of a protein synthesis inhibitor, chloramphenicol. It is assumed that phytochrome system participates in stress-protective mechanisms of the photosynthetic apparatus to UV-radiation.
[Mh] Termos MeSH primário: Arabidopsis/fisiologia
Complexo de Proteínas do Centro de Reação Fotossintética/efeitos da radiação
Fitocromo A/deficiência
Fitocromo B/deficiência
Raios Ultravioleta
[Mh] Termos MeSH secundário: Criptocromos
Luz
Mutação
Fotossíntese/efeitos da radiação
Complexo de Proteína do Fotossistema II/efeitos da radiação
Fitocromo A/genética
Fitocromo B/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cryptochromes); 0 (Photosynthetic Reaction Center Complex Proteins); 0 (Photosystem II Protein Complex); 0 (Phytochrome A); 136250-22-1 (Phytochrome B)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170426
[Lr] Data última revisão:
170426
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE


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[PMID]:27586644
[Au] Autor:Kreslavski VD; Kosobryukhov AA; Schmitt FJ; Semenova GA; Shirshikova GN; Khudyakova AY; Allakhverdiev SI
[Ad] Endereço:Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region, 142290, Russia. vkreslav@rambler.ru.
[Ti] Título:Photochemical activity and the structure of chloroplasts in Arabidopsis thaliana L. mutants deficient in phytochrome A and B.
[So] Source:Protoplasma;254(3):1283-1293, 2017 May.
[Is] ISSN:1615-6102
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The reduced content of photoreceptors, such as phytochromes, can decrease the efficiency of photosynthesis and activity of the photosystem II (PSII). For the confirmation of this hypothesis, the effect of deficiency in both phytochromes (Phy) A and B (double mutant, DM) in 7-27-day-old Arabidopsis thaliana plants on the photosynthetic activity was studied in absence and presence of UV-A radiation as a stress factor. The DM with reduced content of apoproteins of PhyA and PhyB and wild type (WT) plants with were grown in white and red light (WL and RL, respectively) of high (130 µmol quanta m s ) and low (40 µmol quanta m s ) intensity. For DM and WT grown in WL, no notable difference in the photochemical activity of PSII was observed. However, the resistance of the photosynthetic apparatus (PA) to UV-A and the rate of photosynthesis under light saturation were lower in the DM compared to those in the WT. Growth in RL, when the photoreceptors of blue light-cryptochromes-are inactive, resulted in the significant decrease of the photochemical activity of PSII in DM compared to that in WT including amounts of Q -non-reducing complexes of PSII and noticeable enhancement of thermal dissipation of absorbed light energy. In addition, marked distortion of the thylakoid membrane structure was observed for DM grown in RL. It is suggested that not only PhyA and PhyB but also cryptochromes are necessary for normal functioning of the PA and formation of the mechanisms of its resistance to UV-radiation.
[Mh] Termos MeSH primário: Arabidopsis/metabolismo
Cloroplastos/metabolismo
Fotossíntese/fisiologia
Complexo de Proteína do Fotossistema II/metabolismo
Fitocromo A/deficiência
Fitocromo B/deficiência
[Mh] Termos MeSH secundário: Arabidopsis/genética
Cloroplastos/ultraestrutura
Criptocromos/metabolismo
Luz
Processos Fotoquímicos
Fotorreceptores de Plantas/metabolismo
Fitocromo A/genética
Fitocromo B/genética
Folhas de Planta/metabolismo
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cryptochromes); 0 (Photoreceptors, Plant); 0 (Photosystem II Protein Complex); 0 (Phytochrome A); 136250-22-1 (Phytochrome B)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160903
[St] Status:MEDLINE
[do] DOI:10.1007/s00709-016-1020-9


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[PMID]:27908239
[Au] Autor:Hetmann A; Wujak M; Kowalczyk S
[Ad] Endereço:Nicolaus Copernicus University, Faculty of Biology and Environment Protection, Department of Biochemistry, Torun 87-100, Poland. mag_wuj@umk.pl.
[Ti] Título:Protein Transphosphorylation During the Mutual Interaction between Phytochrome A and a Nuclear Isoform of Nucleoside Diphosphate Kinase Is Regulated by Red Light.
[So] Source:Biochemistry (Mosc);81(10):1153-1162, 2016 Oct.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The nuclear isoform of nucleoside diphosphate kinase isoenzyme NDPK-I undergoes strong catalytic activation upon its interaction with the active form of phytochrome A (Pfr) in red light. The autophosphorylation or intermolecular transphosphorylation of NDPK-I leads to the formation of phosphoester bonds stable in acidic solution. The phosphate residue of the phosphamide bond in the active center of NDPK-I can also be transferred to serine and threonine residues localized in other proteins, including phytochrome A. Phytochrome A, similarly to NDPK-I , undergoes autophosphorylation on serine and threonine residues and can phosphorylate some potential substrate proteins. The physical interaction between phytochrome A in the Pfr form and NDPK-I results in a significant increase in the kinase activity of NDPK-I . The results presented in this work indicate that NDPK-I may function as a protein kinase regulated by light.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/enzimologia
Luz
Núcleosídeo-Difosfato Quinase/metabolismo
Fitocromo A/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Proteínas de Arabidopsis/genética
Núcleosídeo-Difosfato Quinase/genética
Fosforilação/genética
Fosforilação/efeitos da radiação
Fitocromo A/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Phytochrome A); EC 2.7.4.6 (Nucleoside-Diphosphate Kinase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161203
[St] Status:MEDLINE


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[PMID]:27820825
[Au] Autor:Sánchez-Lamas M; Lorenzo CD; Cerdán PD
[Ad] Endereço:Fundación Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina.
[Ti] Título:Bottom-up Assembly of the Phytochrome Network.
[So] Source:PLoS Genet;12(11):e1006413, 2016 Nov.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plants have developed sophisticated systems to monitor and rapidly acclimate to environmental fluctuations. Light is an essential source of environmental information throughout the plant's life cycle. The model plant Arabidopsis thaliana possesses five phytochromes (phyA-phyE) with important roles in germination, seedling establishment, shade avoidance, and flowering. However, our understanding of the phytochrome signaling network is incomplete, and little is known about the individual roles of phytochromes and how they function cooperatively to mediate light responses. Here, we used a bottom-up approach to study the phytochrome network. We added each of the five phytochromes to a phytochrome-less background to study their individual roles and then added the phytochromes by pairs to study their interactions. By analyzing the 16 resulting genotypes, we revealed unique roles for each phytochrome and identified novel phytochrome interactions that regulate germination and the onset of flowering. Furthermore, we found that ambient temperature has both phytochrome-dependent and -independent effects, suggesting that multiple pathways integrate temperature and light signaling. Surprisingly, none of the phytochromes alone conferred a photoperiodic response. Although phyE and phyB were the strongest repressors of flowering, both phyB and phyC were needed to confer a flowering response to photoperiod. Thus, a specific combination of phytochromes is required to detect changes in photoperiod, whereas single phytochromes are sufficient to respond to light quality, indicating how phytochromes signal different light cues.
[Mh] Termos MeSH primário: Apoproteínas/genética
Proteínas de Arabidopsis/genética
Arabidopsis/genética
Fitocromo B/genética
Fitocromo/genética
[Mh] Termos MeSH secundário: Apoproteínas/metabolismo
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/metabolismo
Genótipo
Germinação/genética
Luz
Fitocromo/metabolismo
Fitocromo A
Fitocromo B/metabolismo
Folhas de Planta/genética
Folhas de Planta/crescimento & desenvolvimento
Plântulas/genética
Plântulas/crescimento & desenvolvimento
Transdução de Sinais/genética
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apoproteins); 0 (Arabidopsis Proteins); 0 (PHYA protein, Arabidopsis); 0 (PHYB protein, Arabidopsis); 0 (PHYE protein, Arabidopsis); 0 (Phytochrome A); 0 (phytochrome C, Arabidopsis); 11121-56-5 (Phytochrome); 136250-22-1 (Phytochrome B); 158379-16-9 (PHYD protein, Arabidopsis)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161108
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006413


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[PMID]:27794221
[Au] Autor:Kharshiing E; Sinha SP
[Ad] Endereço:Department of Botany, St. Edmund's College, Meghalaya 793 003, India. Electronic address: eros.kharshiing@gmail.com.
[Ti] Título:Deficiency in phytochrome A alters photosynthetic activity, leaf starch metabolism and shoot biomass production in tomato.
[So] Source:J Photochem Photobiol B;165:157-162, 2016 Dec.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Photosynthesis is a key process that promotes plant growth and development. Light provides photosynthetic organisms with a major source of energy to fix carbon dioxide into organic matter. Of the entire visible light spectrum, red/blue light are known to maximise photosynthetic performance and are thus essential for proper growth and development of plants. Red and blue light stimulate synthesis of chlorophyll and orchestrate the positioning of leaves and chloroplasts for optimal utilisation of light, both of which are critical for photosynthesis. The response of plants to external light cues is accomplished via finely tuned complex photoreceptors and signaling mechanisms which enable them to continually monitor light availability and quality for optimal utilisation of light energy towards enhancing their growth. Higher plants contain a suite of photoreceptor proteins that allow them to perceive red, blue/UV-A and UV-B light. Analyses of the phyA mutant of tomato deficient in the red-light photoreceptor phytochrome A (phyA), showed reduced photosynthetic activity of isolated chloroplasts along with decreased shoot biomass in adult plants. The regulation of leaf transitory starch in the mutant was also altered as compared to the wild type (cv Moneymaker). Our results suggest a possible role for phyA in these processes in tomato.
[Mh] Termos MeSH primário: Biomassa
Lycopersicon esculentum/metabolismo
Fotossíntese
Fitocromo A/metabolismo
Folhas de Planta/metabolismo
Brotos de Planta/metabolismo
Amido/metabolismo
[Mh] Termos MeSH secundário: Perfilação da Expressão Gênica
Lycopersicon esculentum/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phytochrome A); 9005-25-8 (Starch)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE


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[PMID]:27208503
[Au] Autor:Eckstein A; Jagiello-Flasinska D; Lewandowska A; Hermanowicz P; Appenroth KJ; Gabrys H
[Ad] Endereço:Department of Plant Biotechnology, Faculty of Biophysics, Biochemistry and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland. Electronic address: aleksandra.eckstein@uj.edu.pl.
[Ti] Título:Mobilization of storage materials during light-induced germination of tomato (Solanum lycopersicum) seeds.
[So] Source:Plant Physiol Biochem;105:271-281, 2016 Aug.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to analyze the metabolism of storage materials in germinating tomato (Solanum lycopersicum) seeds and to determine whether it is regulated by light via phytochromes. Wild type, single and multiple phytochrome A, B1 and B2 mutants were investigated. Imbibed seeds were briefly irradiated with far-red or far-red followed by red light, and germinated in darkness. Triacylglycerols and starch were quantified using biochemical assays in germinating seeds and seedlings during the first 5 days of growth. To investigate the process of fat-carbohydrate transformation, the activity of the glyoxylate cycle was assessed. Our results confirm the role of phytochrome in the control of tomato seed germination. Phytochromes A and B2 were shown to play specific roles, acting antagonistically in far-red light. While the breakdown of triacylglycerols proceeded independently of light, phytochrome control was visible in the next stages of the lipid-carbohydrate transformation. The key enzymes of the glyoxylate cycle, isocitrate lyase and malate synthase, were regulated by phytochrome(s). This was reflected in a greater increase of starch content during seedling growth in response to additional red light treatment. This study is the first attempt to build a comprehensive image of storage material metabolism regulation by light in germinating dicotyledonous seeds.
[Mh] Termos MeSH primário: Germinação/efeitos da radiação
Luz
Lycopersicon esculentum/efeitos da radiação
Sementes/embriologia
[Mh] Termos MeSH secundário: Transporte Biológico/efeitos da radiação
Glioxilatos/metabolismo
Lipídeos
Lycopersicon esculentum/genética
Mutação/genética
Fitocromo A/metabolismo
Fitocromo B/metabolismo
Proteínas de Plantas/metabolismo
Plântulas/metabolismo
Plântulas/efeitos da radiação
Sementes/metabolismo
Amido/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glyoxylates); 0 (Lipids); 0 (Phytochrome A); 0 (Plant Proteins); 136250-22-1 (Phytochrome B); 9005-25-8 (Starch); JQ39C92HH6 (glyoxylic acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160522
[St] Status:MEDLINE


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[PMID]:27143545
[Au] Autor:Yoo J; Cho MH; Lee SW; Bhoo SH
[Ad] Endereço:Graduate School of Biotechnology.
[Ti] Título:Phytochrome-interacting ankyrin repeat protein 2 modulates phytochrome A-mediated PIF3 phosphorylation in light signal transduction.
[So] Source:J Biochem;160(4):243-249, 2016 Oct.
[Is] ISSN:1756-2651
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Light signals recognized by phytochromes are transduced through interactions between down-stream signaling components. Phytochrome-interacting ankyrin repeat protein 2 (PIA2) was found to interact with phytochrome interacting factor 3 (PIF3), a well-known repressor of plant photomorphogenesis in response to phytochrome-mediated light signalling. Both PIA2 and PIF3 are known to be positive regulators of anthocyanin accumulation in Arabidopsis seedlings under far-red conditions. Thus, we investigated the functional relationship between PIA2 and PIF3 in light signalling. We found that PIA2 suppressed PIF3 phosphorylation by phyA. To elucidate how PIA2 modulates phyA-mediated PIF3 phosphorylation, we generated non-phosphorylation mutants and N-terminal α-helix breaking mutants of PIA2. PIF3 phosphorylation by phyA was not suppressed by α-helix breaking PIA2 mutants. The α-helix breaking mutations also resulted in remarkably decreased interactions between PIA2 and PIF3. However, the non-phosphorylation mutants exhibited no effect on phyA-mediated PIF3 phosphorylation. In addition, decreased anthocyanin accumulation in pia2 knockout plant seedlings was not rescued by overexpression of the α-helix breaking mutant in transgenic plants under far-red conditions. These results suggest that PIA2 modulates phyA-mediated PIF3 phosphorylation by physical interaction with PIF3 and that the secondary structure of the PIA2 N-terminus is important in this modulation.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Luz
Fitocromo A/metabolismo
Transdução de Sinais/fisiologia
[Mh] Termos MeSH secundário: Repetição de Anquirina
Arabidopsis/genética
Proteínas de Arabidopsis/genética
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Fosforilação/fisiologia
Fitocromo A/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Basic Helix-Loop-Helix Transcription Factors); 0 (PIF3 protein, Arabidopsis); 0 (Phytochrome A)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160505
[St] Status:MEDLINE


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[PMID]:27030359
[Au] Autor:Wang H; Wu G; Zhao B; Wang B; Lang Z; Zhang C; Wang H
[Ad] Endereço:Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing, 100081, China.
[Ti] Título:Regulatory modules controlling early shade avoidance response in maize seedlings.
[So] Source:BMC Genomics;17:269, 2016 Mar 31.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Optimization of shade avoidance response (SAR) is crucial for enhancing crop yield in high-density planting conditions in modern agriculture, but a comprehensive study of the regulatory network of SAR is still lacking in monocot crops. RESULTS: In this study, the genome-wide early responses in maize seedlings to the simulated shade (low red/far-red ratio) and also to far-red light treatment were transcriptionally profiled. The two processes were predominantly mediated by phytochrome B and phytochrome A, respectively. Clustering of differentially transcribed genes (DTGs) along with functional enrichment analysis identified important biological processes regulated in response to both treatments. Co-expression network analysis identified two transcription factor modules as potentially pivotal regulators of SAR and de-etiolation, respectively. A comprehensive cross-species comparison of orthologous DTG pairs between maize and Arabidopsis in SAR was also conducted, with emphasis on regulatory circuits controlling accelerated flowering and elongated growth, two physiological hallmarks of SAR. Moreover, it was found that the genome-wide distribution of DTGs in SAR and de-etiolation both biased toward the maize1 subgenome, and this was associated with differential retention of various cis-elements between the two subgenomes. CONCLUSIONS: The results provide the first transcriptional picture for the early dynamics of maize phytochrome signaling. Candidate genes with regulatory functions involved in maize shade avoidance response have been identified, offering a starting point for further functional genomics investigation of maize adaptation to heavily shaded field conditions.
[Mh] Termos MeSH primário: Adaptação Fisiológica/genética
Redes Reguladoras de Genes
Luz
Plântulas/crescimento & desenvolvimento
Zea mays/genética
[Mh] Termos MeSH secundário: Arabidopsis/genética
Análise por Conglomerados
Regulação da Expressão Gênica de Plantas
Genoma de Planta
Fitocromo A/genética
Fitocromo B/genética
RNA de Plantas/genética
Sequências Reguladoras de Ácido Nucleico
Plântulas/genética
Análise de Sequência de RNA
Fatores de Transcrição/genética
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Phytochrome A); 0 (RNA, Plant); 0 (Transcription Factors); 136250-22-1 (Phytochrome B)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160401
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-016-2593-6



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