Base de dados : MEDLINE
Pesquisa : D08.811.913.696.620.682.700.646.500.984 [Categoria DeCS]
Referências encontradas : 773 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 78 ir para página                         

  1 / 773 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26991553
[Au] Autor:Prasad S; Koch B; Chaube SK
[Ad] Endereço:Department of Zoology, Biochemistry Unit, Cell Physiology Laboratory, Varanasi, 221005, U.P., India.
[Ti] Título:Maturation promoting factor destabilization facilitates postovulatory aging-mediated abortive spontaneous egg activation in rat.
[So] Source:Dev Growth Differ;58(3):293-302, 2016 Apr.
[Is] ISSN:1440-169X
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The present study was designed to investigate whether destabilization of maturation promoting factor (MPF) leads to postovulatory aging-mediated abortive spontaneous egg activation (SEA). If so, we wished to determine whether changes in Wee-1 as well as Emi2 levels are associated with MPF destabilization during postovulatory aging-mediated abortive SEA in rats eggs aged in vivo. For this purpose, sexually immature female rats were given a single injection (20 IU IM) of pregnant mare serum gonadotropin for 48 h followed by single injection of human chorionic gonadotropin (20 IU). Ovulated eggs were collected after 14, 17, 19 and 21 h post-hCG surge to induce postovulatory aging in vivo. The morphological changes, Wee1, phosphorylation status of cyclin dependent kinase 1(Cdk1), early mitotic inhibitor 2 (Emi2), anaphase promoting complex/cyclosome (APC/C), cyclin B1, mitotic arrest deficient protein (MAD2) levels and Cdk1 activity were analyzed. The increased Wee 1 level triggered phosphorylation of Thr-14/Tyr-15 and dephosphorylation of Thr-161 residues of Cdk1. The decrease of Emi2 level was associated with increased APC/C level and decreased cyclin B1 level. Changes in phosphorylation status of Cdk1 and reduced cyclin B1 level resulted in destabilization of MPF. The destabilized MPF finally led to postovulatory aging-mediated abortive SEA in rat eggs. It was concluded that the increase of Wee 1 but decrease of Emi2 level triggers MPF destabilization and thereby postovulatory aging-mediated abortive SEA in rat eggs.
[Mh] Termos MeSH primário: Senescência Celular/fisiologia
Fator Promotor de Maturação/metabolismo
Oócitos/fisiologia
Ovulação/fisiologia
[Mh] Termos MeSH secundário: Ciclossomo-Complexo Promotor de Anáfase
Animais
Proteína Quinase CDC2/metabolismo
Gonadotropina Coriônica/farmacologia
Ciclina B1/metabolismo
Proteínas F-Box/metabolismo
Feminino
Gonadotropinas Equinas/farmacologia
Cavalos
Seres Humanos
Proteínas Mad2/metabolismo
Microscopia de Fluorescência
Oócitos/citologia
Oócitos/metabolismo
Ovulação/efeitos dos fármacos
Fosforilação
Proteínas Tirosina Quinases/metabolismo
Ratos
Treonina/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chorionic Gonadotropin); 0 (Cyclin B1); 0 (Emi2 protein, rat); 0 (F-Box Proteins); 0 (Gonadotropins, Equine); 0 (Mad2 Proteins); 2ZD004190S (Threonine); EC 2.3.2.27 (Anaphase-Promoting Complex-Cyclosome); EC 2.7.10.- (Wee1 protein, rat); EC 2.7.10.1 (Protein-Tyrosine Kinases); EC 2.7.11.22 (CDC2 Protein Kinase); EC 2.7.11.22 (Maturation-Promoting Factor)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160319
[St] Status:MEDLINE
[do] DOI:10.1111/dgd.12272


  2 / 773 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26982431
[Au] Autor:Prasad S; Koch B; Chaube SK
[Ad] Endereço:1 Cell Physiology Laboratory, Biochemistry Unit, Department of Zoology, Institute of Science, Banaras Hindu University , Varanasi-221005, Uttar Pradesh, India .
[Ti] Título:Involvement of Cyclin-Dependent Kinase 1 during Postovulatory Aging-Mediated Abortive Spontaneous Egg Activation in Rat Eggs Cultured In Vitro.
[So] Source:Cell Reprogram;18(2):96-107, 2016 Apr.
[Is] ISSN:2152-4998
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Freshly ovulated rat eggs do not remain arrested at metaphase II (MII) and undergo exit from MII arrest with initiation of extrusion of the second polar body (PBII), a characteristic feature of abortive spontaneous egg activation (SEA). The biochemical and molecular changes during postovulatory aging-mediated abortive SEA remain poorly understood. We investigated the morphological, cellular, and molecular changes during postovulatory aging-mediated abortive SEA in eggs cultured in vitro. Our results suggest that postovulatory egg aging in vitro induced initiation of PBII extrusion in a time-dependent manner. Postovulatory aging increased Wee1 kinase and Thr-14/Tyr-15 phosphorylated cyclin-dependent kinase 1 (Cdk1) levels, whereas Thr-161 phosphorylated Cdk1 and cyclin B1 levels were significantly decreased in eggs cultured in vitro. The early mitotic inhibitor 2 (Emi2) level was significantly reduced, but anaphase promoting complex/cyclosome (APC/C) and mitotic arrest deficient protein (MAD2) levels were increased initially and then reduced during a later period of in vitro culture. These results suggest that an increased Wee1 kinase level modulated the specific phosphorylation status of Cdk1, increased Cdk1 activity, and decreased the cyclin B1 level. Furthermore, the decreased Emi2 level was associated with an increased level of APC/C and decreased level of cyclin B1, which resulted in maturation promoting factor (MPF) destabilization and finally led to postovulatory aging-mediated abortive SEA in rat eggs cultured in vitro.
[Mh] Termos MeSH primário: Pontos de Checagem do Ciclo Celular
Senescência Celular
Quinases Ciclina-Dependentes/metabolismo
Oócitos/enzimologia
[Mh] Termos MeSH secundário: Animais
Proteína Quinase CDC2
Técnicas de Cultura de Células
Feminino
Fator Promotor de Maturação/metabolismo
Oócitos/citologia
Proteínas Tirosina Quinases/metabolismo
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 2.7.10.- (Wee1 protein, rat); EC 2.7.10.1 (Protein-Tyrosine Kinases); EC 2.7.11.22 (CDC2 Protein Kinase); EC 2.7.11.22 (Cdk1 protein, rat); EC 2.7.11.22 (Cyclin-Dependent Kinases); EC 2.7.11.22 (Maturation-Promoting Factor)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160317
[St] Status:MEDLINE
[do] DOI:10.1089/cell.2015.0068


  3 / 773 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26918273
[Au] Autor:Stricker SA; Beckstrom B; Mendoza C; Stanislawski E; Wodajo T
[Ad] Endereço:Department of Biology, MSC03 2020, 1 University of New Mexico, Albuquerque, NM, 87131, USA.
[Ti] Título:Oocyte aging in a marine protostome worm: The roles of maturation-promoting factor and extracellular signal regulated kinase form of mitogen-activated protein kinase.
[So] Source:Dev Growth Differ;58(3):250-9, 2016 Apr.
[Is] ISSN:1440-169X
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The roles of maturation-promoting factor (MPF) and an extracellular signal regulated kinase form of mitogen-activated protein kinase (ERK MAPK) are analyzed during oocyte aging in the marine protostome worm Cerebratulus. About a day after removal from the ovary, unfertilized metaphase-I-arrested oocytes of Cerebratulus begin to flatten and swell before eventually lysing, thereby exhibiting characteristics of a necroptotic mode of regulated cell death. Based on immunoblots probed with phospho-specific antibodies, MPF and ERK are initially active in freshly mature specimens. However, as oocytes age, both kinase activities decline, with ERK deactivation occurring well before MPF downregulation. Experiments using pharmacological modulators indicate that oocyte degradation is promoted by the maturation-initiated activation of ERK as well as by the deactivation of MPF that occurs in extensively aged specimens. The potential significance of these findings is discussed relative to previously published results for apoptotic eggs and oocytes of echinoderm and vertebrate deuterostomes.
[Mh] Termos MeSH primário: Senescência Celular/fisiologia
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Invertebrados/metabolismo
Fator Promotor de Maturação/metabolismo
Oócitos/fisiologia
[Mh] Termos MeSH secundário: Animais
Apoptose/fisiologia
Butadienos/farmacologia
Células Cultivadas
Senescência Celular/efeitos dos fármacos
Colforsina/farmacologia
MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores
Feminino
Immunoblotting
Invertebrados/citologia
Invertebrados/efeitos dos fármacos
Fator Promotor de Maturação/antagonistas & inibidores
Nitrilos/farmacologia
Oócitos/citologia
Oócitos/efeitos dos fármacos
Inibidores de Proteínas Quinases/farmacologia
Purinas/farmacologia
Água do Mar/parasitologia
Fatores de Tempo
Vasodilatadores
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Butadienes); 0 (Nitriles); 0 (Protein Kinase Inhibitors); 0 (Purines); 0 (U 0126); 0 (Vasodilator Agents); 0ES1C2KQ94 (roscovitine); 1F7A44V6OU (Colforsin); EC 2.7.11.22 (Maturation-Promoting Factor); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160227
[St] Status:MEDLINE
[do] DOI:10.1111/dgd.12269


  4 / 773 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26464247
[Au] Autor:Luo YB; Zhang L; Lin ZL; Ma JY; Jia J; Namgoong S; Sun QY
[Ad] Endereço:State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Beijing, China.
[Ti] Título:Distinct subcellular localization and potential role of LINE1-ORF1P in meiotic oocytes.
[So] Source:Histochem Cell Biol;145(1):93-104, 2016 Jan.
[Is] ISSN:1432-119X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:LINE-1 is an autonomous non-LTR retrotransposon in mammalian genomes and encodes ORF1P and ORF2P. ORF2P has been clearly identified as the enzyme supplier needed in LINE-1 retrotransposition. However, the role of ORF1P is not well explored. In this study, we employed loss/gain-of-function approach to investigate the role of LINE1-ORF1P in mouse oocyte meiotic maturation. During mouse oocyte development, ORF1P was observed in cytoplasm as well as in nucleus at germinal vesicle (GV) stage while was localized on the spindle after germinal vesicle breakdown (GVBD). Depletion of ORF1P caused oocyte arrest at the GV stage as well as down-regulation of CDC2 and CYCLIN B1, components of the maturation-promoting factor (MPF). Further analysis demonstrated ORF1P depletion triggered DNA damage response and most of the oocytes presented altered chromatin configuration. In addition, SMAD4 showed nuclear foci signal after Orf1p dsRNA injection. ORF1P overexpression held the oocyte development at MI stage and the chromosome alignment and spindle organization were severely affected. We also found that ORF1P could form DCP1A body-like foci structure in both cytoplasm and nucleus after heat shock. Taken together, accurate regulation of ORF1P plays an essential role in mouse oocyte meiotic maturation.
[Mh] Termos MeSH primário: Elementos Nucleotídeos Longos e Dispersos/genética
Meiose/genética
Oócitos/citologia
Oogênese/fisiologia
Proteínas de Ligação a RNA/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteína Quinase CDC2/metabolismo
Ciclina B1/metabolismo
Reparo do DNA/genética
Endorribonucleases/metabolismo
Feminino
Fator Promotor de Maturação/metabolismo
Camundongos
Camundongos Endogâmicos ICR
RNA Mensageiro/genética
Proteínas de Ligação a RNA/genética
Proteína Smad4/metabolismo
Fuso Acromático/metabolismo
Transativadores/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ccnb1 protein, mouse); 0 (Cyclin B1); 0 (ECAT11 protein, mouse); 0 (RNA, Messenger); 0 (RNA-Binding Proteins); 0 (Smad4 Protein); 0 (Smad4 protein, mouse); 0 (Trans-Activators); EC 2.7.11.22 (CDC2 Protein Kinase); EC 2.7.11.22 (Maturation-Promoting Factor); EC 3.1.- (Endoribonucleases); EC 3.1.- (smad4-interacting protein SMIF, mouse)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151015
[St] Status:MEDLINE
[do] DOI:10.1007/s00418-015-1369-4


  5 / 773 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26006336
[Au] Autor:Arias Torres AJ; Bühler MI; Zelarayán LI
[Ad] Endereço:Instituto de Biología,Facultad de Bioqca,Qca. y Farmacia,INSIBIO-UNT,Chacabuco 461,Tucumán,Argentina.
[Ti] Título:In vitro steroid-induced meiosis in Rhinella arenarum oocytes: role of pre-MPF activation.
[So] Source:Zygote;24(2):252-8, 2016 Apr.
[Is] ISSN:1469-8730
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this work we showed the relationship between seasonal periods and the response of R. arenarum follicles and oocytes to different steroids. Using in vitro germinal vesicle breakdown (GVBD) assays, we demonstrated that P4 is the main steroid capable of inducing maturation in R. arenarum oocytes and follicles. In the second part of this work we showed that androgens can activate pre-maturation promoting factors (pre-MPFs) such as P4, by cytoplasm microinjection experiments. The results indicated that the steroids assayed induced oocyte and follicle maturation in a dose- and time-dependent manner. In oocytes, P4 was the most efficient steroid as a maturation inducer (EC50 of the reproductive period, 6 nM, EC50 of the non-reproductive period ≅ 30 nM). Androgens (DHEA, dehydroepiandrosterone; T, testosterone; and AD, androstenedione) were less efficient maturation inducers than P4 (EC50 reproductive period ≅ 50, 120 and 600 nM respectively). Similar results were obtained with intact follicles in both seasonal periods. Although the response of follicles to the different androgens was variable, in no case was it above the above the response induced by P4. Independently of the season, oocytes and follicles incubated in P4, P5 and T underwent GVBD after 6-10 h while oocytes and follicles incubated in DHEA and AD matured more slowly. Furthermore, we demonstrated that microinjection of mature cytoplasm from androgen-treated oocytes is sufficient to promote GVBD in immature recipient oocytes (DHEA, 57 ± 12%; AD, 60 ± 8%; T, 56 ± 13%). Thus, androgens such as DHEA, T and AD are as competent as P4 to activate pre-MPF.
[Mh] Termos MeSH primário: Hormônios Esteroides Gonadais/farmacologia
Fator Promotor de Maturação/metabolismo
Meiose/efeitos dos fármacos
Oócitos/efeitos dos fármacos
Precursores de Proteínas/metabolismo
[Mh] Termos MeSH secundário: Androgênios/administração & dosagem
Androgênios/farmacologia
Animais
Bufo arenarum
Células Cultivadas
Citoplasma/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ativação Enzimática/efeitos dos fármacos
Feminino
Hormônios Esteroides Gonadais/administração & dosagem
Técnicas de Maturação in Vitro de Oócitos/métodos
Microinjeções
Oócitos/citologia
Oócitos/fisiologia
Oogênese/efeitos dos fármacos
Folículo Ovariano/citologia
Folículo Ovariano/efeitos dos fármacos
Folículo Ovariano/fisiologia
Progesterona/administração & dosagem
Progesterona/farmacologia
Estações do Ano
Testosterona/administração & dosagem
Testosterona/farmacologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Androgens); 0 (Gonadal Steroid Hormones); 0 (Protein Precursors); 0 (maturation-promoting factor precursor); 3XMK78S47O (Testosterone); 4G7DS2Q64Y (Progesterone); EC 2.7.11.22 (Maturation-Promoting Factor)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150527
[St] Status:MEDLINE
[do] DOI:10.1017/S0967199415000143


  6 / 773 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26400307
[Au] Autor:Wang LM; Lv WW; Zuo D; Dong ZJ; Zhao YL
[Ad] Endereço:Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi, China.
[Ti] Título:Characteristics of Cyclin B and its potential role in regulating oogenesis in the red claw crayfish (Cherax quadricarinatus).
[So] Source:Genet Mol Res;14(3):10786-98, 2015 Sep 09.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Cyclin B is a regulatory subunit of maturation-promoting factor (MPF), which has a key role in the induction of meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, the complete cDNA sequence of Cyclin B was cloned from the red claw crayfish, Cherax quadricarinatus, and its spatiotemporal expression profiles were analyzed. Cyclin B cDNA (1779 bp) encoded a 401 amino acid protein with a calculated molecular weight of 45.1 kDa. Quantitative real-time PCR demonstrated that Cyclin B mRNA was expressed mainly in the ovarian tissue and that the expression decreased as the ovaries developed. Immunofluorescence analysis revealed that the Cyclin B protein relocated from the cytoplasm to the nucleus during oogenesis. These findings suggest that Cyclin B plays an important role in gametogenesis and gonad development in C. quadricarinatus.
[Mh] Termos MeSH primário: Astacoidea/genética
Ciclina B/genética
Regulação da Expressão Gênica no Desenvolvimento
Fator Promotor de Maturação/genética
Oócitos/metabolismo
Oogênese/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Astacoidea/citologia
Astacoidea/crescimento & desenvolvimento
Sequência de Bases
Núcleo Celular/metabolismo
Clonagem Molecular
Ciclina B/metabolismo
Citoplasma/metabolismo
DNA Complementar/genética
DNA Complementar/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Feminino
Fator Promotor de Maturação/metabolismo
Meiose
Dados de Sequência Molecular
Peso Molecular
Oócitos/citologia
Oócitos/crescimento & desenvolvimento
Fases de Leitura Aberta
Ovário/citologia
Ovário/crescimento & desenvolvimento
Ovário/metabolismo
Transporte Proteico
Reação em Cadeia da Polimerase em Tempo Real
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cyclin B); 0 (DNA, Complementary); 0 (Recombinant Proteins); EC 2.7.11.22 (Maturation-Promoting Factor)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:150924
[Lr] Data última revisão:
150924
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150925
[St] Status:MEDLINE
[do] DOI:10.4238/2015.September.9.17


  7 / 773 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26090895
[Au] Autor:Alam MJ; Kumar S; Singh V; Singh RK
[Ad] Endereço:School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi-110067, India; College of Applied Medical Sciences, University of Hail, Hail-2440, Kingdom of Saudi Arabia.
[Ti] Título:Bifurcation in Cell Cycle Dynamics Regulated by p53.
[So] Source:PLoS One;10(6):e0129620, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We study the regulating mechanism of p53 on the properties of cell cycle dynamics in the light of the proposed model of interacting p53 and cell cycle networks via p53. Irradiation (IR) introduce to p53 compel p53 dynamics to suffer different phases, namely oscillating and oscillation death (stabilized) phases. The IR induced p53 dynamics undergo collapse of oscillation with collapse time Δt which depends on IR strength. The stress p53 via IR drive cell cycle molecular species MPF and cyclin dynamics to different states, namely, oscillation death, oscillations of periods, chaotic and sustain oscillation in their bifurcation diagram. We predict that there could be a critical Δtc induced by p53 via IRc, where, if Δt〈Δtc the cell cycle may come back to normal state, otherwise it will go to cell cycle arrest (apoptosis).
[Mh] Termos MeSH primário: Ciclo Celular/fisiologia
Proteína Supressora de Tumor p53/metabolismo
[Mh] Termos MeSH secundário: Ciclo Celular/efeitos da radiação
Ciclinas/metabolismo
Fator Promotor de Maturação/metabolismo
Modelos Biológicos
Ligação Proteica
Proteínas Proto-Oncogênicas c-mdm2/metabolismo
Radiação Ionizante
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cyclins); 0 (Tumor Suppressor Protein p53); EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2); EC 2.7.11.22 (Maturation-Promoting Factor)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150620
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0129620


  8 / 773 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25788662
[Au] Autor:German SD; Lee JH; Campbell KH; Sweetman D; Alberio R
[Ad] Endereço:Division of Animal Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom.
[Ti] Título:Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes.
[So] Source:Biol Reprod;92(4):103, 2015 Apr.
[Is] ISSN:1529-7268
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Oocytes treated with the protein synthesis inhibitor cycloheximide (CHX) arrest at the germinal vesicle (GV) stage and undergo accelerated GV breakdown (GVBD) after CHX is removed. However, little is known about the underlying mechanism of accelerated meiotic maturation. Here, we investigated this mechanism and found that oocytes released from CHX arrest have higher amounts of cyclin B1 (CCNB1) and phosphorylated mitogen-activated protein kinase (pMAPK) proteins. Increased levels of these factors were not associated with mRNA polyadenylation or increased transcription rates of CCNB1 and MOS (Moloney murine sarcoma viral oncogene homolog) during CHX arrest. We found that treatment of CHX-arrested oocytes with the actin filament-stabilizing agent Jasplakinolide (Jasp) delayed GVBD following release from CHX arrest and that this was correlated with reduced maturation-promoting factor (MPF) activity. These results suggest that CCNB1 mRNAs released from actin filaments during CHX arrest increase CCNB1 transcripts available for translation after release from CHX arrest, leading to the precocious activation of MPF and accelerated meiotic progression.
[Mh] Termos MeSH primário: Actinas/metabolismo
Cicloeximida/farmacologia
Meiose/efeitos dos fármacos
Oócitos/efeitos dos fármacos
Inibidores da Síntese de Proteínas/farmacologia
[Mh] Termos MeSH secundário: Animais
Ciclo Celular/efeitos dos fármacos
Ciclina B1/metabolismo
Depsipeptídeos/farmacologia
Feminino
Fator Promotor de Maturação/farmacologia
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Vírus do Sarcoma Murino de Moloney/genética
Técnicas de Transferência Nuclear
Polimerização
Gravidez
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins); 0 (Cyclin B1); 0 (Depsipeptides); 0 (Protein Synthesis Inhibitors); 102396-24-7 (jasplakinolide); 98600C0908 (Cycloheximide); EC 2.7.11.22 (Maturation-Promoting Factor); EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150424
[Lr] Data última revisão:
150424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150320
[St] Status:MEDLINE
[do] DOI:10.1095/biolreprod.114.122341


  9 / 773 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25712366
[Au] Autor:Kishimoto T
[Ad] Endereço:Laboratory of Cell and Developmental Biology, Graduate School of Bioscience, Tokyo Institute of Technology, Yokohama, 226-8501, Japan. kishimoto.takeo@ocha.ac.jp.
[Ti] Título:Entry into mitosis: a solution to the decades-long enigma of MPF.
[So] Source:Chromosoma;124(4):417-28, 2015 Dec.
[Is] ISSN:1432-0886
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Maturation or M phase-promoting factor (MPF) is the universal inducer of M phase common to eukaryotic cells. MPF was originally defined as a transferable activity that can induce the G2/M phase transition in recipient cells. Today, however, MPF is assumed to describe an activity that exhibits its effect in donor cells, and furthermore, MPF is consistently equated with the kinase cyclin B-Cdk1. In some conditions, however, MPF, as originally defined, is undetectable even though cyclin B-Cdk1 is fully active. For over three decades, this inconsistency has remained a long-standing puzzle. The enigma is now resolved through the elucidation that MPF, defined as an activity that exhibits its effect in recipient cells, consists of at least two separate kinases, cyclin B-Cdk1 and Greatwall (Gwl). Involvement of Gwl in MPF can be explained by its contribution to the autoregulatory activation of cyclin B-Cdk1 and by its stabilization of phosphorylations on cyclin B-Cdk1 substrates, both of which are essential when MPF induces the G2/M phase transition in recipient cells. To accomplish these tasks, Gwl helps cyclin B-Cdk1 by suppressing protein phosphatase 2A (PP2A)-B55 that counteracts cyclin B-Cdk1. MPF, as originally defined, is thus not synonymous with cyclin B-Cdk1, but is instead a system consisting of both cyclin B-Cdk1 that directs mitotic entry and Gwl that suppresses the anti-cyclin B-Cdk1 phosphatase. The current view that MPF is a synonym for cyclin B-Cdk1 in donor cells is thus imprecise; instead, MPF is best regarded as the entire pathway involved in the autoregulatory activation of cyclin B-Cdk1, with specifics depending on the experimental system.
[Mh] Termos MeSH primário: Pontos de Checagem da Fase G2 do Ciclo Celular/fisiologia
Fator Promotor de Maturação/fisiologia
Mitose/fisiologia
[Mh] Termos MeSH secundário: Animais
Ciclina B
Eucariotos
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Cyclin B); EC 2.7.11.22 (Maturation-Promoting Factor)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150226
[St] Status:MEDLINE
[do] DOI:10.1007/s00412-015-0508-y


  10 / 773 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25604742
[Au] Autor:Prasad S; Tiwari M; Tripathi A; Pandey AN; Chaube SK
[Ad] Endereço:Cell Physiology Laboratory, Biochemistry Unit, Department of Zoology, Banaras Hindu University, Varanasi, 221005, India.
[Ti] Título:Changes in signal molecules and maturation promoting factor levels associate with spontaneous resumption of meiosis in rat oocytes.
[So] Source:Cell Biol Int;39(6):759-69, 2015 Jun.
[Is] ISSN:1095-8355
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study was aimed to find out changes in signal molecules and maturation promoting factor (MPF) levels during meiotic cell cycle progression from diplotene and metaphase-II (M-II) arrest, a period during which oocyte achieves meiotic competency. Data suggest that high levels of adenosine 3'-5'-cyclic monophosphate (cAMP), guanosine 3'-5'-cyclic monophosphate (cGMP), and nitric oxide (NO) are associated with diplotene arrest, while reduction in their levels correlates with reduced MPF level and meiotic resumption from diplotene arrest. On the other hand, increased intracellular NO, calcium (Ca(2+) ) as well as hydrogen peroxide (H2 O2 ) levels correlate with decreased cAMP, Thr-161 phosphorylated cyclin-dependent kinase-1 (Cdk1) as well as cyclin B1 levels. The decreased Thr-161 phosphorylated Cdk1 and cyclin B1 level reduce MPF level leading to exit from M-II arrest in oocytes cultured in vitro. These data suggest that the decrease of cAMP level and increase of cytosolic free Ca(2+) as well as H2 O2 levels associate with the reduced MPF level and meiotic resumption from diplotene arrest. On the other hand, increase of NO, cGMP, Ca(2+) as well as H2 O2 levels are associated with reduced MPF and spontaneous exit from M-II arrest in rat oocytes cultured in vitro.
[Mh] Termos MeSH primário: Fator Promotor de Maturação/metabolismo
Meiose
Oócitos/citologia
Oócitos/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Proteína Quinase CDC2/metabolismo
Cálcio/metabolismo
Forma Celular
Cromossomos de Mamíferos/metabolismo
AMP Cíclico/metabolismo
GMP Cíclico/metabolismo
Ciclina B1/metabolismo
Citosol/metabolismo
Feminino
Fluorescência
Peróxido de Hidrogênio/metabolismo
Óxido Nítrico/metabolismo
Nitritos/metabolismo
Fosforilação
Fosfotreonina/metabolismo
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cyclin B1); 0 (Nitrites); 1114-81-4 (Phosphothreonine); 31C4KY9ESH (Nitric Oxide); BBX060AN9V (Hydrogen Peroxide); E0399OZS9N (Cyclic AMP); EC 2.7.11.22 (CDC2 Protein Kinase); EC 2.7.11.22 (Maturation-Promoting Factor); H2D2X058MU (Cyclic GMP); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150518
[Lr] Data última revisão:
150518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150122
[St] Status:MEDLINE
[do] DOI:10.1002/cbin.10444



página 1 de 78 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde